Growth inhibitor for fungi of the genus Malassezia

Abstract

The objective is to provide an inhibitor of growth against fungi of the genus Malassezia. [Solution] Hibiscus flower extract, ivy leaf / stem extract, royal jelly extract, tench Scutellaria baicalensis extract, Scutellaria baicalensis extract, N-[2-hydroxy-3-(trimethylammonium) chloride The active ingredient is at least one selected from the group consisting of propyl hydrolyzed keratin solution. It is a growth inhibitor against Malassezia fungi. Applicable to hair.

Description

TECHNICAL FIELD

[0001] The present invention relates to a growth inhibitor against fungi of the genus Malassezia.

BACKGROUND ART

[0002] Fungi of the genus Malassezia are yeast-like fungi that normally reside on human skin, and their growth requires li pids, making them so-called lipophilic fungi. Depending on the condition of the host, they are also known as causative or exacerbating bacteria for skin diseases such as tinea versicolor, seborrheic der matitis, Malassezia folliculitis, atopic dermatitis, psoriasis, and acne. Currently, fungi of the genus Malassezia are classified into 18 species, and M alassezia globosa and Malassezia restricta are species that are inevitably detected on the skin of patients with skin diseases or healthy individuals (Non-Patent Documents 1 and 2). Among the above diseases, for example, as therapeutic agents for seborrheic dermatitis, topical steroids, non-ste roidal anti-inflammatory topical agents, antifungal agents such as ketoconazole, and the like are known (Non-Patent Document 3 ).

PRIOR ART DOCUMENTS

NON-PATENT DOCUMENTS

[0003]

NON-PATENT DOCUMENT 1

NON-PATENT DOCUMENT 2

NON-PATENT DOCUMENT 3

SUMMARY OF THE INVENTION

PROBLEMS TO BE SOLVED BY THE INVENTION

[0004] ​​ However, Malassezia fungi, especially Malassezia, which are detected on human skin For sezia globosa and Malassezia restricta, directly Not many drugs have been reported that effectively inhibit proliferation. Therefore, to treat, improve, and prevent diseases and symptoms associated with fungi of the genus Malassezia There is a need for an active ingredient that can achieve this. To solve the above-mentioned problems of the conventional method, the present invention addresses fungi of the genus Malassezia. The objective is to provide a growth inhibitor. [Means for solving the problem]

[0005] The inventors of this invention have conducted extensive research to solve the above problems and have found that hibiscus flower extract, Hedera helix (Ivy) leaf / stem extract, royal jelly extract, Tencha extract, Scutellaria baicalensis extract N-[2-hydroxy-3-(trimethylammonio)propyl chloride] hydrolyzed keratin At least one species selected from the group consisting of liquids is a fungus of the genus Malassezia, particularly Ma It has been found to be suitable for a novel application: the ability to suppress the proliferation of Lassezia globosa. And thus, the present invention was completed.

[0006] In other words, the present invention is (1) Hibiscus flower extract, Hedera helix leaf / stem extract, Royal jelly extract, Te Tea extract, Scutellaria baicalensis extract, N-[2-hydroxy-3-(trimethylammonium chloride) The active ingredient is selected from the group consisting of (O)propyl hydrolyzed keratin solution and A growth inhibitor for fungi of the genus Malassezia, characterized by containing the following: (2) For use on skin and / or hair against the Malassezia fungi described in (1) A growth inhibitor, (3) The fungus of the genus Malassezia is Malassezia globosa, as described in (1 ) or (2), the growth inhibitor according to any one of the preceding claims, is provided.

Effects of the Invention

[0007] According to the present invention, it has become possible to provide a growth inhibitor for fungi of the genus Malassezia .

Modes for Carrying Out the Invention

[0008] The growth inhibitor for fungi of the genus Malassezia of the present invention contains at least one selected from the group consisting of Hibiscus flower extract, English ivy leaf / stem extract, royal jelly extract, tencha extract, Scutellaria root extract, and hydrolyzed keratin solution of N-[2-hydroxy-3-(trimethylammonio)propyl] chloride, and is intended for use on the skin and / or hair. The above-mentioned respective agents can be appropriately used with quality that is ordinarily employed in pharmaceuticals, quasi-pharmaceutical products, or cosmetics. The Hibiscus flower extract of the present invention is an extract extracted from the flowers of Roselle (scientific name: Hibiscus sabdariffa), a plant belonging to the Malvaceae family. The extraction method for the above extract is not particularly limited, and can be carried out according to a conventional method. As the extraction solvent used for extraction, any of polar solvents and non-polar solvents can be used, and for example, water, ethanol, propylene glycol, 1,3-butylene glycol, and mixed solutions thereof can be used. The above extract may be used as the extracted solution as it is, and if necessary, may be used after being subjected to treatments such as concentration, dilution, filtration, decolorization with activated carbon or the like, deodorization, and ethanol precipitation.

[0009] The extracted solution may further be concentrated ​​​​​​may be subjected to treatments such as concentration to dryness, spray drying, freeze drying and the like, and used as a dried product. Fur thermore, a commercially available product can also be used.

[0010] The blending amount of the hibiscus flower extract in the Malassezia fungus growth inhibitor of the present invention is preferably 0.0001 mass% to 20 mass%, more preferably 0.001 mass% relative to the total composition when provided as a pharmaceutical, quasi-drug, cosmetic, or reagent, respectively, relative to the total composition respectively, preferably 0.0001 mass% to 20 mass%, more preferably 0.001 mass % to 10 mass%.

[0011] The English ivy leaf / stem extract of the present invention is an extract extracted from the leaves and stems of the plant named English ivy (scientific name Hedera helix) belonging to the Araliaceae family. The extraction method for the above extract is not particularly lim ited, and can be performed according to a conventional method. As the extraction solvent used for extraction, any of a polar sol vent and a non-polar solvent can be used, and examples thereof include water, ethanol, propylene gly col, 1,3-butylene glycol, and mixed solutions thereof. The above extra ct may be used as the extracted solution as it is, and if necessary, may be used after being subjected to treatments such as concentration, dilution, filtration, decolorization with activated carbon or the like, deodorization, ethanol precipitation and the like. Furthermore, the extracted solution may be subjected to treatments such as concentration to dryness, spray drying, freeze drying and the like, and used as a dried product. The extracted solution is subjected to treatments such as concentration to dryness, spray drying, freeze drying and the like, and used as a dried product A commercially available product can also be used.

[0012] The blending amount of the English ivy leaf / stem ex tract in the Malassezia fungus growth inhibitor of the present invention, when provided as a pharmaceutical, quasi-drug, cosmetic, or reagent, is based on the total composition based on the total composition, preferably 0.0001 mass% to 20 mass% relative to the total composition , more preferably 0.001 mass% to 10 mass%.

[0013] The royal jelly extract of the present invention is derived from European honeybees (Apis mellifera L.) or It is produced in vivo from pollen and nectar of the honeybee (Apis indika Radoszkowski), and in the pharynx. The extract is obtained from secretions secreted from glands or mandibular glands. The extraction method of the above extract is There are no particular limitations, and it can be carried out according to conventional methods. The extraction solvent used for extraction is: Both polar and nonpolar solvents can be used, for example, water, ethanol, and propylene glycol. Glycol, 1,3-butylene glycol, and mixtures thereof can be used. The above extract may be used as is, or it may be concentrated, diluted, filtered, and activated as needed. You may also use materials that have been treated with decolorization, deodorization, or ethanol precipitation using carbon or similar methods. Furthermore, The extracted solution is subjected to treatments such as concentration to dryness, spray drying, and freeze-drying to obtain a dried product. You may use it. You can also use commercially available products.

[0014] The present invention relates to a growth inhibitor of Malassezia fungi, and the royal jelly extract The amount of each ingredient, when provided as a pharmaceutical, quasi-drug, cosmetic, or reagent, is calculated for the entire composition. In contrast, it is preferably 0.0001% by mass to 20% by mass, and more preferably 0.001% by mass. It is between % by quantity and 10% by mass.

[0015] The Tencha extract of this invention is derived from the plant known as sweet tea (scientific name Rubus suavissimus), which belongs to the Rosaceae family. This is an extract obtained from the leaves. The method of extracting the above extract is not particularly limited and can be done by any conventional method. This can be done. The extraction solvent used can be either a polar solvent or a nonpolar solvent. It can also be used, for example, water, ethanol, propylene glycol, 1,3-butylene glycol Glycols and mixtures thereof can be used. The above extract is the extracted solution. It can be used as is, and if necessary, it can be concentrated, diluted, filtered, decolorized with activated carbon, deodorized, or ethanol-free. You may also use a solution that has been treated with a solution such as Nol precipitation. Furthermore, the extracted solution may be concentrated to dryness and sprayed. It may also be used as a dried product after processing such as drying or freeze-drying. Alternatively, commercially available products may be used. It is possible to stay there.

[0016] Amount of Tencha extract in the growth inhibitor of Malassezia fungi of the present invention When provided as a pharmaceutical, quasi-drug, cosmetic, or reagent, the following applies to the entire composition: Preferably 0.0001% by mass to 20% by mass, and more preferably 0.001% by mass to It is 10% by mass.

[0017] The scutellaria baicalensis extract of this invention is derived from the plant *Scutellaria baicalensis*, which belongs to the Lamiaceae family. This is an extract from the root of *ensis*. The extraction method for the above extract is not particularly limited and is always It can be carried out in accordance with the law. The extraction solvent used may be a polar solvent or a non-polar solvent. Any of the following can be used as a medium, for example, water, ethanol, propylene glycol, 1, 3-butylene glycol and mixtures thereof can be used. The above extract is extracted The solution can be used as is, or, if necessary, it can be concentrated, diluted, filtered, decolorized with activated carbon, etc. You may also use a solution that has been treated with deodorization, ethanol precipitation, etc. Furthermore, the extracted solution can be concentrated. It may also be used as a dried product after being subjected to treatments such as drying, spray drying, or freeze-drying. Commercially available products can also be used.

[0018] Amount of Scutellaria baicalensis extract in the growth inhibitor of Malassezia fungi of the present invention When provided as a pharmaceutical, quasi-drug, cosmetic, or reagent, the following applies to the entire composition: Preferably 0.0001% by mass to 20% by mass, and more preferably 0.001% by mass to It is 10% by mass.

[0019] The present invention provides N-[2-hydroxy-3-(trimethylammonio)propyl]hydrolyzed chloride. The keratin solution is extracted while maintaining the amino acid balance of the keratin protein derived from wool. • This is purified α-keratose that has been cationized. Use the extracted and purified solution as is. It is also fine to concentrate, dilute, filter, decolorize with activated carbon, deodorize, and precipitate with ethanol as needed. Processed materials such as those mentioned above may also be used. Furthermore, the extracted solution may be concentrated to dryness, spray-dried, or frozen. It may also be used as a dried product after processing such as drying. Alternatively, commercially available products can be used. can.

[0020] The present invention relates to a growth inhibitor for Malassezia fungi, specifically N-[2-hydroxychlorohydrate The amount of C-3-(trimethylammonio)propyl hydrolyzed keratin solution included is for pharmaceuticals. When provided as a quasi-drug, cosmetic, or reagent, preferably 0% of the total composition. The mass is between 0.0001% and 20%, and more preferably between 0.001% and 10%. be.

[0021] The Malassezia genus fungus of the present invention is Malassezia globos a, Malassezia restricta, Malassezia furfur , Malassezia sympodialis, Malassezia pachy Examples include dermatis, but preferably Malassezia globo It is sa.

[0022] The present invention provides a growth inhibitor for fungi of the genus Malassezia, preferably for use on human skin. It is used for (the scalp, including) or for hair. [Examples]

[0023] The present invention will be further described below with reference to examples and test examples, but the present invention is These examples are not limiting in any way.

[0024] (Test Example 1: Growth inhibition test of Malassezia globosa) To evaluate the inhibitory effect on growth of Malassezia globosa, the following method was used. It was tested according to the law. (Preparation of test media) The culture medium was prepared so that the final concentration would be as follows: Artificial sebum mimicking human scalp sebum 2 %(Triolein 58.6%, Glyceryl Distearate 15.6%, Palmitic Acid 6%) 1%, Myristic acid 2.2%, Pentadecanoic acid 1.5%, Oleic acid 4.7%, Squalane (11.3% mixture), glucose 1%, bile salt 0.8%, yeast Extract 0.2%, Glycerin Monostelate 0.05%, Glycerin 1%, Mix 1% of self-emulsifying glyceryl monostearate and sterilize, then chromoagar candidiasis (Kanto region) A predetermined amount of chemical was added and used. (Preparation of inoculum solution) Malassezia globosa was pre-cultured in modified LNA medium (32 The samples were left standing for 3 days at °C, and the resulting test bacteria were collected using a sterile loop, suspended in PBS, and washed. The bacterial suspension after washing was measured for a bacterial load of approximately 10 7Inoculate with PBS diluted to a CFU / mL concentration. It was used as a bacterial solution. (Preparation of test substance) Hibiscus flower extract, ivy leaf / stem extract, royal jelly extract, tencha Extract, Scutellaria baicalensis extract, N-[2-hydroxy-3-(trimethylammonium) chloride [Ropil] Hydrolyzed keratin solution and 1,3-butylene glycol are diluted four times with purified water. It was filtered and sterilized. Also, the hibiscus flower extract used was organic hibiscus. (Manufactured by Maruzen Pharmaceutical Co., Ltd.), Hedera helix leaf / stem extract is Hedera helix extract BG-J (Maruzen Pharmaceutical Co., Ltd.) (Manufactured by), Royal Jelly Extract is Royal Jelly Extract B (manufactured by Ichimaru Falcos), Tencha The extract is Sweet Tea Extract BGW (manufactured by Maruzen Pharmaceutical Co., Ltd.), and the Scutellaria Baicalensis Extract is Scutellaria Baicalensis Extract BG-JC (manufactured by Maruzen Co., Ltd.). (Pharmaceutical product), N-[2-hydroxy-3-(trimethylammonio)propyl chloride] hydrolyzed The keratin solution is Proticute C Alpha (manufactured by Ichimaru Falcos). (Antibacterial testing) The test substance, 1,3-butylene glycol, and After adding 200 μL each of purified water as a positive control, the prepared artificial sebum was added. The culture medium was added in 300 μL increments and quickly mixed. Note that 1,3-butylene glycol was dissolved. A control medium was used. After the culture medium solidified, 10 μL of the inoculum was added to the center of each medium. The cells were cultured at 32°C for 3 days. Growth was facilitated by the chromogenic enzyme substrate contained in Chromoagar Candida. The bacteria that were exposed to the solution turned red, allowing for comparison of growth in the positive control and solvent control with the bacteria in each pore. The growth was visually compared, and the survival rates of the bacteria were measured at 0% and 25% when the positive control was set to 100%. The results were rated on a 5-point scale: 50%, 75%, and 100%.

[0025] (Test results) Table 1 shows the average survival rates of bacteria tested with n=2 for each case.

[0026] [Table 1]

[0027] As is clear from Table 1, hibiscus flower extract, ivy leaf / stem extract, rhododendron Jelly extract, Tencha extract, Scutellaria baicalensis extract, N-[2-hydroxy-3- (trimethylammonio)propyl hydrolyzed keratin solution is all solvent-controlled. It has been confirmed that it has a higher growth inhibitory effect than 1,3-butylene glycol, and it is found in hibiscus flowers. Extracts, Hedera helix leaf / stem extract, royal jelly extract, Tencha extract, Ougo Extract, N-[2-hydroxy-3-(trimethylammonio)propyl]hydrochloride At least one species selected from the group consisting of keratin solutions is Malassezia glob It was suggested that it has an inhibitory effect on the proliferation of OSA. [Industrial applicability]

[0028] The growth inhibitor of Malassezia fungi of the present invention suppresses the abnormal proliferation of Malassezia fungi. It is expected to suppress and normalize the imbalance of commensal bacteria on the skin, scalp, or hair. The present invention provides a growth inhibitor for fungi of the genus Malassezia. Fungal diseases, such as tinea versicolor, seborrheic dermatitis, Malassezia folliculitis, and atopic dermatitis. Cosmetics and quasi-drugs for the treatment, improvement, and prevention of skin diseases and symptoms such as psoriasis and acne. It can be used in the pharmaceutical field.

Claims

1. Hibiscus flower extract, ivy leaf / stem extract, royal jelly extract, tench Scutellaria baicalensis extract, Scutellaria baicalensis extract, N-[2-hydroxy-3-(trimethylammonium) chloride] At least one selected from the group consisting of propyl hydrolyzed keratin solution is used as an active ingredient. A growth inhibitor for fungi of the genus Malassezia, characterized by containing this agent.

2. For use on skin and / or hair, the product for use against Malassezia fungi according to claim 1. A growth inhibitor.

3. Claim that the aforementioned fungus of the genus Malassezia is Malassezia globesa. A growth inhibitor according to 1 or 2.

Images