Fully automated dPCR system and method of use thereof

Abstract

A fully automated digital polymerase chain reaction (dPCR) system is provided. The system includes a sample preparation unit configured to receive a biological sample and extract nucleic acids therefrom, an analysis vessel, a partitioner configured to generate a plurality of sample partitions containing the extracted nucleic acids and distribute the generated sample partitions among the analysis vessels, a thermocycler configured to adjust the temperature of the generated sample partitions to amplify the nucleic acids of interest in the generated sample partitions, and a sample reader configured to detect whether the amplified nucleic acids are present in each of the generated sample partitions in the analysis vessel. The system also includes an anti-adhesion coating configured to at least reduce binding of the extracted nucleic acids to the dPCR system. Methods and kits for carrying out the invention are also provided.

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