Manipulated RNA polymerase variant

Abstract

This disclosure relates to engineered RNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding engineered RNA polymerase polypeptides. The disclosure also provides methods for using engineered RNA polymerase polypeptides or compositions thereof to produce RNA. In further embodiments, the disclosure provides an expression vector comprising at least one recombinant polynucleotide encoding an engineered RNA polymerase described herein. In some embodiments, the recombinant polynucleotide in the expression vector is operably ligated to a control sequence. In some embodiments, the control sequence comprises a promoter, particularly a heterologous promoter.

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