Copper dehydrogenases derived from multi-copper oxidases and their bioelectrochemical applications
By modifying copper dehydrogenases of multi-copper oxidases to reduce oxidative activity and enhance dehydrogenative activity, the problems of poor stability and oxygen competition for electron transfer of tyrosinase and horseradish peroxidase are solved, achieving efficient and selective detection of L-DOPA, which is suitable for the treatment and management of Parkinson's disease patients.
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- THE UNIV OF NORTH CAROLINA AT CHAPEL HILL
- Filing Date
- 2024-05-30
- Publication Date
- 2026-07-07
AI Technical Summary
Existing tyrosinases and horseradish peroxidases exhibit poor stability and are easily inhibited by reaction products when monitoring phenolic compounds, and require hydrogen peroxide for catalysis, limiting their practical applications; in the case of native multi-copper oxidases, oxygen competes for electrons during electron transfer, affecting electrode reaction efficiency.
To develop a copper dehydrogenase that reduces oxidative activity and enhances dehydrogenation activity by modifying a multi-copper oxidase, particularly by modifying the amino acid residues of the T2 and T3 copper ligands to improve selectivity and sustained detection of substrates such as L-DOPA.
It enables efficient and selective detection of L-DOPA, which is suitable for the treatment and management of Parkinson's disease patients, improves the accuracy and safety of drug management, and reduces the risk of drug toxicity.
Smart Images
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