Method for producing purine nucleoside phosphorylase mutants and 2'-fluoronucleosides
Purine nucleoside phosphorylase mutants with enhanced activity address the low yield issue, enabling efficient 2'-fluoronucleoside production suitable for industrial applications.
JP2026524179APending Publication Date: 2026-07-21JINLIN ASYMCHEM PHARM CO LTD +1
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Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- JINLIN ASYMCHEM PHARM CO LTD
- Filing Date
- 2023-10-31
- Publication Date
- 2026-07-21
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Figure 2026524179000001_ABST
Abstract
The present invention provides a purine nucleoside phosphorylase mutant and a method for producing 2'-fluoronucleosides. Here, the purine nucleoside phosphorylase mutant includes (a) a protein in which one or more mutations selected from L148, P19, Q41, etc. have occurred based on the wild-type purine nucleoside phosphorylase enzyme GsPNP represented by SEQ ID NO: 3; or (b) a protein having 70% or more homology to the amino acid sequence limited in (a) and possessing purine nucleoside phosphorylase activity. This invention can solve the problem of low synthesis yield of 2'-fluoronucleosides by enzyme catalysts in the prior art and is suitable for the field of enzyme catalysis.
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