Compositions suitable for use in skincare

Abstract

This invention relates to skincare compositions. In particular, it relates to skincare compositions comprising myrrh resin extract and retinoids. This invention also relates to methods for treating skin using compositions and methods for enhancing the anti-aging effects of retinoids in the skin layer.

Description

TECHNICAL FIELD

[0001] (CROSS-REFERENCE TO RELATED APPLICATIONS) This application claims the benefit of U.S. Provisional Patent Application No. 63 / 588,030 filed on October 5, 2023, the entire disclosure of which is incorporated herein by reference for all purposes.

[0002] (FIELD OF THE INVENTION) The present invention relates generally to skincare compositions. In particular, the present invention relates to a skincare composition comprising a myrrh resin extract and a retinoid. The present invention also relates to a method of treating skin using the above composition.

BACKGROUND ART

[0003] Human skin undergoes certain aging processes, some of which are attributed to intrinsic processes (e.g., chronological aging) and some to extrinsic factors (e.g., photoaging). In addition, temporary or even persistent changes to the skin, such as acne, oily or dry skin, keratosis, rosacea, photosensitivity, inflammatory, erythematous, and allergic or autoimmune reactive reactions, for example, dermatitis and photodermatosis, may occur.

[0004] Consequences of the aforementioned aging processes may include skin thinning, weakening of the interlacement of the epidermis and dermis, as well as a reduction in the number of cells and the blood vessels that supply them. These consequences are often undesirable, and individuals suffering from these issues turn to topical treatments to address them.

[0005] Retinoids have been used to treat skin conditions caused by intrinsic aging, extrinsic factors, acne or skin disorders. However, retinoids can be difficult to formulate, as many are susceptible to photodegradation, and retinoids may be overly irritating to the skin of some individuals.

[0006] Myrrh resin is a natural extract from the Commiphora genus of trees. Its uses in perfumes and medicines, such as as a disinfectant, have been known since ancient times. Topical application of myrrh resin to the skin can cause skin irritation.

[0007] One option to avoid irritation caused by retinoids on the skin is to suggest alternative compounds that activate the same or similar biological pathways as retinoids. However, this is a complex task because identifying such alternative compounds is difficult. Another option is to enhance or increase the beneficial effects of retinoids and thus limit the amount of retinoid applied topically. However, this task is also complex because identifying these compounds is difficult.

[0008] There is a continuing need and interest in identifying compounds or products that can enhance or augment the activation of retinoids to beneficial biological pathways without increasing the undesirable side effects of retinoids, such as skin irritation. Improving the potential of retinoids using known, reliable natural ingredients is particularly desirable. [Overview of the project] [Means for solving the problem]

[0009] One aspect of the present invention relates to a skincare composition comprising myrrh resin extract and a retinoid.

[0010] This embodiment can be combined with various embodiments and in any combination. Therefore, in some embodiments, the myrrh resin extract is produced from the resin of a plant of the genus Commiphora. In one or more embodiments, the myrrh resin extract is obtained by extraction in an organic solvent. In some embodiments, the myrrh resin extract is present in an amount ranging from about 0.000001% to about 10% of the total weight of the composition. In one or more embodiments, the retinoid is selected from the group consisting of retinol, retinal, retinol esters, retinoic acid, their derivatives, and combinations thereof. In one or more embodiments, the retinoid is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition. In one or more embodiments, the ratio of myrrh resin to retinoid is about 100:1 to about 1:10. In some embodiments, the skincare composition further comprises components selected from the group consisting of surfactants, chelating agents, emollients, moisturizers, conditioners, preservatives, opacifiers, fragrances, and combinations of two or more of these. In one or more embodiments, the skincare composition is in the form of a solution, suspension, emulsion, lotion, cream, serum, gel, stick, spray, ointment, liquid cleanser, bar soap, shampoo, hair conditioner, paste, foam, powder, mousse, shaving cream, hydrogel, or film-forming product.

[0011] Another aspect of the present invention relates to a skincare composition comprising about 0.01% to about 2.0% by weight of myrrh resin extract and about 0.01% to about 1.0% by weight of retinol, based on the total weight of the composition.

[0012] Another aspect of the present invention relates to a method for treating skin, comprising topically applying a composition according to any of the above embodiments to the skin.

[0013] Another aspect of the present invention relates to a method for treating signs of skin aging, comprising topically applying a skincare composition according to any of the above embodiments to skin that requires treatment for skin aging.

[0014] Another aspect of the present invention relates to a method for enhancing the anti-aging effect of retinoids in the skin layers of the skin where it is needed, and comprising topically applying a skincare composition comprising myrrh resin extract and retinoids to the skin.

[0015] Another aspect of the present invention relates to a method for increasing CRABP2 or AQP3 expression in skin cells, comprising topically applying a skincare composition comprising myrrh resin extract and a retinoid to the skin.

[0016] This embodiment can be combined with various embodiments and in any combination. Therefore, in some embodiments of the methods disclosed above, the retinoid is selected from the group consisting of retinol, retinal, retinol esters, retinoic acid, their derivatives, and combinations thereof. In one or more embodiments, retinol is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

[0017] In one or more embodiments, the myrrh resin extract is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

[0018] In one or more embodiments, a skincare composition applied topically to the skin comprises about 0.01% to about 2.0% by weight of myrrh resin extract and about 0.01% to about 1.0% by weight of retinol, based on the total weight of the composition.

[0019] These and other features and advantages of the present invention will be readily apparent from the following "Modes for Carrying Out the Invention". [Modes for carrying out the invention]

[0020] As used herein, a skincare product or skincare composition is a product that can be applied to the user's skin or hair and provides benefits to that user. These benefits may include, for example, hydration of the skin or hair, or delivery of one or more therapeutic or beneficial agents to the skin or hair, as described in more detail below.

[0021] To provide a more accurate explanation, some of the quantitative expressions presented herein are not qualified with the term “approximately”. Whether the term “approximately” is explicitly used or not, all quantities given herein are meant to refer to actual given values, and also to approximate values ​​of such given values ​​that are reasonably estimated on the basis of ordinary skill in the art, including approximations of such given values ​​by experimental and / or measurement conditions. General conventions in scientific and technical literature apply: the last decimal place of a number indicates its precision. Where no other margin of error is given, the maximum margin is confirmed by applying the convention of rounding to the last decimal place; for example, for a measurement of 3.5%, the margin of error is 3.45 to 3.54.

[0022] All percentages (%) described herein are by weight unless otherwise specified.

[0023] One aspect of the present invention is, Myrrh resin extract, This invention relates to a skincare composition containing retinoids.

[0024] Surprisingly, this combination of myrrh resin extract and retinoids has been found to provide increased CRABP2 or AQP3 expression in skin cells without the drawbacks, such as skin irritation, conventionally associated with natural extracts such as retinoids. This increase in CRABP2 or AQP3 expression is thought to have beneficial effects on the skin. For example, it can be used to reduce signs of skin aging or to increase skin hydration.

[0025] "Extract" means a substance or a mixture of substances that is removed from myrrh resin by a chemical process. The chemical process can be, for example, the treatment of myrrh resin with a solvent to remove the soluble substance or mixture of soluble substances from the myrrh resin. Accordingly, "myrrh resin extract" is a subset of myrrh resin.

[0026] In one or more embodiments, the skin care composition may comprise a myrrh resin extract that is produced from the resin of a plant of the genus Commiphora.

[0027] Preferably, it is obtained from the resin of plants such as Commiphora Myrrha and Commiphora molmol.

[0028] In this embodiment, "produced" means that the myrrh resin is a secretion obtained from the plant bark.

[0029] The natural origin of the myrrh resin extract is a further advantage, as most consumers are looking for active ingredients of natural origin.

[0030] In one or more embodiments, the myrrh resin extract is obtained by extraction with an organic solvent.

[0031] Myrrh resin is a complex mixture of chemical compounds, and it has been discovered that a fraction of myrrh resin isolated by extraction with an organic solvent such as a polar organic solvent provides the beneficial effects according to the present invention.

[0032] Preferably, the organic extraction of myrrh resin is carried out using an alcohol solvent such as methanol, ethanol, propanol, isopropanol, butanol, preferably ethanol.

[0033] The resin obtained from the bark of a Commiphora tree is treated with an organic solvent such as ethanol to obtain a fraction of the resin, referred to as the "myrrh resin extract" according to the present invention.

[0034] Myrrh resin extract may also be blended with triethylacetyl citrate; for example, the myrrh resin extract may contain 10% to 20%, preferably 12% to 15%, of triethylacetyl citrate.

[0035] In one or more embodiments, the myrrh resin extract is present in an amount ranging from about 0.000001% to about 10% of the total weight of the composition.

[0036] Preferably, the myrrh resin extract is present in an amount ranging from about 0.000001% to about 1% of the total weight of the composition, or about 0.000001% to about 0.5% of the total weight of the composition, or about 0.000001% to about 0.2% of the total weight of the composition, or about 0.000001% to about 0.1% of the total weight of the composition, or about 0.000001% to about 0.01% of the total weight of the composition, or about 0.00001% to about 2% of the total weight of the composition, or about 0.0001% to about 2% of the total weight of the composition, or about 0.001% to about 2% of the total weight of the composition, or about 0.01% to about 1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount exceeding 0.001% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount greater than 0.005% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount greater than 0.01% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.5% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.2% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.05% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.02% of the total weight of the composition.

[0037] In one or more embodiments, the myrrh resin extract is in a free, unencapsulated form. Alternatively, the myrrh resin extract may be in an encapsulated form.

[0038] Preferably, the myrrh resin extract is not encapsulated.

[0039] In one or more embodiments, the retinoid is selected from the group consisting of retinol, retinal, retinol esters, retinoic acid, derivatives thereof, and combinations thereof.

[0040] In a preferred embodiment, the retinoid is retinol.

[0041] In one or more embodiments, the retinoid is in a free, unencapsulated form. Alternatively, the retinoid may be in an encapsulated form.

[0042] In preferred embodiments, the retinoid is not encapsulated.

[0043] In one or more embodiments, the retinoid is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

[0044] Preferably, retinoids are present in an amount ranging from about 0.001% to about 5%, or from about 0.001% to about 1%, or from about 0.001% to about 0.5%. Preferably, retinoids are present in an amount exceeding 0.005% of the total weight of the composition. Preferably, retinoids are present in an amount exceeding 0.01% of the total weight of the composition. Preferably, retinoids are present in an amount exceeding 0.05% of the total weight of the composition. Preferably, retinoids are present in an amount exceeding 0.1% of the total weight of the composition. Preferably, retinoids are present in an amount less than 5% of the total weight of the composition. Preferably, retinoids are present in an amount less than 1% of the total weight of the composition. Preferably, retinoids are present in an amount less than 0.5% of the total weight of the composition.

[0045] In one or more embodiments, the ratio of myrrh resin extract to retinoid is approximately 100:1 to approximately 1:10.

[0046] In certain preferred embodiments, the ratio of myrrh resin extract to retinoid is about 10:1 to about 1:5, preferably about 10:1 to about 1:2, preferably about 5:1 to about 1:1, preferably about 2.5:1 to about 1:1; preferably about 1.25:1.

[0047] In one or more embodiments, the skincare composition according to the present invention may further include components selected from the group consisting of surfactants, chelating agents, emollients, moisturizers, conditioners, preservatives, pearlescent or opacifying agents, fragrances, and two or more combinations thereof.

[0048] The skincare composition contains a surfactant, which may include nonionic, anionic, cationic, or amphoteric surfactants, or a combination of surfactants.

[0049] Suitable nonionic surfactants include, but are not limited to, fatty alcoholic acids or amide ethoxylates, monoglyceride ethoxylates, sorbitan ester ethoxylate alkyl polyglycosides, and mixtures thereof.

[0050] Examples of anionic surfactants include alkyl phosphates, glyceryl alkyl citrates, anionic fatty acids, alkyl sulfates, alkyl sarcosinates, olefin sulfonates, alkyl taurates, or salts of alkyl isethionates.

[0051] Examples of cationic surfactants suitable for use in the present invention include alkyl quaternary ammonium salts (mono, di, tri), benzyl quaternary ammonium salts, ester quaternary ammonium salts, ethoxylated quaternary ammonium salts, alkylamines, and mixtures thereof. In this case, the alkyl group has about 6 to about 30 carbon atoms, but is preferably about 8 to about 22 carbon atoms.

[0052] Examples of amphoteric surfactants suitable for use in the present invention include, but are not limited to, amphocarboxylates such as alkyl amphoacetate (mono or di), alkyl betaine, alkylamide alkyl betaine (other than CAPB), alkylamide alkyl sultaine, alkyl amphophosphate, phosphorylated imidazolines such as phosphobetaine and pyrophosphobetaine, carboxyalkyl alkyl polyamines, alkylimino-dipropionate, alkyl amphoglycinate (mono or di), alkyl amphopropionate (mono or di), N-alkyl β-aminopropionic acid, alkyl polyaminocarboxylate, and mixtures thereof.

[0053] Surfactants, or combinations of surfactants, may be present in a total amount of approximately 5% to 30% by weight, or approximately 10% to 20% by weight, relative to the weight of the skincare composition.

[0054] Skincare compositions may contain emollients, and emollients or emollient blends are useful for cleansing the skin. As used herein, “emollient” means a material used for cleansing the skin, hair, and eyelashes, for preventing or alleviating dryness, or for protecting the skin. Examples of emollients useful herein include, but are not limited to, linear or branched hydrocarbon emollients, e.g., decane, isohexadecane, dodecane, tridecane, tetradecane, squalene, hexadecane, or isoparaffin; hydrophobic compounds, e.g., vegetable oils; mineral oils (e.g., petrolatum); fatty acid esters of glycerol, fatty acid esters including linear or branched alkyl esters (e.g., isopropyl palmitate, C12-C15 alkyl benzoate); stearic acid esters, e.g., isopropyl isostearate, decyl isostearate (including simple isostearate and highly monobranched isostearate decyl, which is an ester formed from isostearate in which the proportion of monobranched molecules is increased compared to simple isostearate), and isostearates including hexyl isostearate; and combinations thereof.

[0055] The skincare composition may contain one emollient or two or more emollients. The emollient(s) may be present in an amount of about 3% to about 15% by weight of the skincare composition, or about 5% to about 10% by weight of the skincare composition.

[0056] The skincare composition may not contain, or substantially does not contain, antimicrobial preservatives. The skincare composition may contain antioxidants, such as tocopheryl acetate. If used, the antioxidant may be present in an amount of about 0.05% to about 0.5% by weight, or about 0.1% to about 0.25% by weight, relative to the weight of the skincare composition.

[0057] The skincare composition may further contain one or more humectants, such as panthenol or glycerin. If used, the humectants may be present in amounts of less than about 1% by weight, less than about 0.5% by weight, or less than about 0.25% by weight.

[0058] The skincare composition may further comprise one or more fragrances or fragrance blends, including essential oils or other fragrance components. The myrrh resin extract according to the present invention is not considered a fragrance. When used, the fragrance is present in a total weight of about 0.001% to about 0.5% by weight of the composition, or about 0.01% to about 0.2% by weight of the composition. The skincare composition may preferably be free from or substantially free from added colorants or dyes. The skincare composition may preferably be opaque and white, avoiding a grayish color.

[0059] Examples of suitable pearlescent or opalescent agents include, but are not limited to, (a) fatty acids having about 16 to about 22 carbon atoms, and (b) mono or diesters of either ethylene or propylene glycol; (a) fatty acids having about 16 to about 22 carbon atoms; (b) mono or diesters of polyalkylene glycols of the formula: HO-(JO)aH (wherein J is an alkylene group having about 2 to about 3 carbon atoms, and a is 2 or 3); aliphatic alcohols containing about 16 to about 22 carbon atoms; aliphatic esters of the formula: KCOOCH2L (wherein K and L independently contain about 15 to about 21 carbon atoms); inorganic solids insoluble in the composition, and mixtures thereof.

[0060] In one or more embodiments, the skincare composition according to the present invention may be in the form of a solution, suspension, emulsion, lotion, cream, serum, gel, stick, spray, ointment, liquid cleanser, bar soap, shampoo, hair conditioner, paste, foam, powder, mousse, shaving cream, hydrogel, or film-forming product.

[0061] Skincare compositions may not substantially contain processed oat ingredients such as oat oil.

[0062] As used herein, “essentially free” or “substantially free” of an ingredient means that the ingredient is present in less than 0.1 weight percent or less than 0.01 weight percent, or is not present at all.

[0063] Another aspect of the present invention is, a. Approximately 0.01% to 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The present invention relates to a skincare composition comprising approximately 0.01% to approximately 1.0% by weight of retinol, based on the total weight of the composition.

[0064] Skincare composition, a. Approximately 0.01% to 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The composition may contain approximately 0.01% to approximately 1.0% by weight of retinol, based on the total weight of the composition. Myrrh resin extract is obtained by extracting the resin of plants of the genus Commiphora with an organic solvent.

[0065] Skincare composition, a. Approximately 0.01% to 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The composition may contain approximately 0.01% to approximately 1.0% by weight of retinol, based on the total weight of the composition. Myrrh resin extract is obtained by extracting the resin of plants of the genus Commiphora with an organic solvent. The ratio of myrrh resin extract to retinol is approximately 10:1 to 1:5, preferably approximately 10:1 to 1:2, preferably approximately 5:1 to 1:1, preferably approximately 2.5:1 to 1:1, and preferably approximately 1.25:1.

[0066] Another aspect of the present invention relates to a method of skin treatment, comprising topically applying a skin care composition according to the present invention to the skin.

[0067] Skin treatment methods can include cosmetic skin treatments.

[0068] Another aspect of the present invention relates to a method for treating signs of skin aging, comprising topically applying a skincare composition according to the present invention to skin that is in need of treatment for skin aging.

[0069] The present invention is suitable for treating signs of skin aging. When used in the present invention, "signs of skin aging" include the presence of fine lines and wrinkles, loss of elasticity, uneven skin, and age spots. In a particularly preferred embodiment, the signs of aging are the presence of fine lines and wrinkles, and / or loss of elasticity.

[0070] As used herein, “wrinkles” include fine wrinkles, fine lines, and coarse lines. Examples of wrinkles include, but are not limited to, fine lines around the eyes (e.g., “crow’s feet”), wrinkles on the forehead and cheeks, frown lines, and nasolabial folds around the mouth.

[0071] As used herein, “loss of elasticity” includes, but is not limited to, sagging, loosening, and flabby tissue, as well as loss of elasticity or structural integrity of skin or tissue. Loss of elasticity or structural integrity of tissue may result from a number of factors, including, but not limited to, disease, aging, hormonal changes, mechanical trauma, environmental damage, or the application of products such as cosmetics or pharmaceuticals to the tissue.

[0072] As used herein, “uneven skin” means a skin condition associated with diffuse or mottled pigmentation, which may be classified as hyperpigmentation, such as post-inflammatory hyperpigmentation.

[0073] As used herein, "blemish" means a skin condition associated with redness or erythema.

[0074] In another embodiment, the present invention may relate to a method for treating acne, comprising topically applying a skincare composition according to the present invention to skin requiring treatment for acne. In a preferred embodiment, the method for treating acne is a cosmetic method for treating acne.

[0075] The present invention is also suitable for the treatment of acne. As used herein, “acne” refers to a disorder resulting from the action of hormones and other substances in the sebaceous glands and hair follicles, typically resulting in clogged pores and the formation of inflammatory or non-inflammatory lesions on the skin. In particular, this relates to wounds, lesions, or pimples, pre-emergent pimples, blackheads, and / or whiteheads. As used herein, “pre-emergent pimples” refers to inflammatory vesicles on the surface of the skin that are not visually apparent to the naked eye (e.g., as lesions).

[0076] The present invention is also suitable for the treatment of rosacea. As used herein, “rosacea” means skin having persistent erythema with or without papules, pustules, or nodules.

[0077] In another embodiment, the present invention may relate to a method for smoothing the texture of skin, comprising topically applying a skincare composition according to the present invention to skin that requires a smoother texture treatment. In a preferred embodiment, the method for smoothing the texture of skin is a cosmetic method of skin texture treatment.

[0078] In another embodiment, the present invention may relate to a method for brightening skin, which includes topically applying a skincare composition according to the present invention to the skin. In a preferred embodiment, the method for brightening skin is a cosmetic method of skin treatment.

[0079] In another embodiment, the present invention may relate to a method for enhancing the anti-aging effect of retinoids in the skin layers of the skin where it is needed, comprising topically applying a skincare composition comprising myrrh resin extract and retinoids to the skin.

[0080] In a preferred embodiment, the method according to the present invention for enhancing the anti-aging effect of retinoids in the skin layer of the skin where it is needed is a cosmetic treatment method.

[0081] "Enhancing anti-aging effects" means that signs of skin aging are reduced and become less visible.

[0082] Another aspect of the present invention relates to a method for increasing CRABP2 or AQP3 expression in skin cells, comprising topically applying a skincare composition comprising myrrh resin extract and a retinoid to the skin.

[0083] In a preferred embodiment, the method for increasing CRABP2 or AQP3 expression in skin cells according to the present invention is a cosmetic treatment method.

[0084] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing CRABP2 or AQP3 expression in skin cells.

[0085] In one or more embodiments, a method for increasing CRABP2 or AQP3 expression in skin cells further comprises a retinoid selected from the group consisting of retinol, retinal, retinol esters, retinoic acid, derivatives thereof, and combinations thereof.

[0086] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing CRABP2 or AQP3 expression in skin cells, and therefore for treating signs of aging, treating acne, smoothing skin texture, or brightening the skin.

[0087] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method to increase CRABP2 or AQP3 expression in skin cells and thus increase skin hydration.

[0088] Another aspect of the present invention relates to a method for increasing CRABP2 expression in skin cells, comprising topically applying a skincare composition comprising myrrh resin extract and a retinoid to the skin.

[0089] In a preferred embodiment, the method for increasing CRABP2 expression in skin cells according to the present invention is a cosmetic treatment method.

[0090] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing CRABP2 expression in skin cells.

[0091] In one or more embodiments, a method for increasing CRABP2 expression in skin cells further comprises a retinoid selected from the group consisting of retinol, retinal, retinol esters, retinoic acid, derivatives thereof, and combinations thereof.

[0092] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing CRABP2 expression in skin cells and therefore treating signs of aging, treating acne, smoothing skin texture, or brightening the skin.

[0093] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing CRABP2 expression in skin cells and thus increasing skin hydration.

[0094] Another aspect of the present invention relates to a method for increasing AQP3 expression in skin cells, comprising topically applying a skincare composition comprising myrrh resin extract and a retinoid to the skin.

[0095] In a preferred embodiment, the method for increasing AQP3 expression in skin cells according to the present invention is a cosmetic treatment method.

[0096] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing AQP3 expression in skin cells and therefore treating signs of aging, treating acne, smoothing skin texture, or brightening the skin.

[0097] In another embodiment, the present invention may relate to a skincare composition comprising myrrh resin extract and a retinoid for use in a cosmetic treatment method for increasing AQP3 expression in skin cells and thus increasing skin hydration.

[0098] In one or more embodiments, a method for increasing AQP3 expression in skin cells comprises a retinoid selected from the group consisting of retinol, retinal, retinol esters, retinoic acid, derivatives thereof, and combinations thereof.

[0099] In one or more embodiments, a method for increasing CRABP2 or AQP3 expression in skin cells comprises retinol present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

[0100] Preferably, retinol is present in an amount ranging from about 0.001% to about 5%, or from about 0.001% to about 1%, or from about 0.001% to about 0.5%. Preferably, retinol is present in an amount greater than 0.005% of the total weight of the composition. Preferably, retinol is present in an amount greater than 0.01% of the total weight of the composition. Preferably, retinol is present in an amount greater than 0.05% of the total weight of the composition. Preferably, retinol is present in an amount greater than 0.1% of the total weight of the composition. Preferably, retinol is present in an amount less than 5% of the total weight of the composition. Preferably, retinol is present in an amount less than 1% of the total weight of the composition. Preferably, retinol is present in an amount less than 0.5% of the total weight of the composition.

[0101] In one or more embodiments, a method for increasing CRABP2 or AQP3 expression in skin cells comprises a myrrh resin extract present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

[0102] Preferably, the myrrh resin extract is present in an amount ranging from about 0.001% to about 1% of the total weight of the composition, or about 0.001% to about 0.5% of the total weight of the composition, or about 0.001% to about 0.2% of the total weight of the composition, or about 0.001% to about 0.1% of the total weight of the composition, or about 0.001% to about 0.01% of the total weight of the composition, or about 0.00001% to about 2% of the total weight of the composition, or about 0.0001% to about 2% of the total weight of the composition, or about 0.001% to about 2% of the total weight of the composition, or about 0.01% to about 1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount exceeding 0.001% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount greater than 0.005% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount greater than 0.01% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.5% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.2% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.05% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.02% of the total weight of the composition.

[0103] In one or more embodiments, a method for increasing CRABP2 or AQP3 expression in skin cells comprises a myrrh resin extract, which is obtained from Commiphora myrrh resin by extraction using an organic solvent.

[0104] In one or more embodiments, a method for increasing CRABP2 or AQP3 expression in skin cells includes topical application of a skincare composition, the skincare composition is a. Approximately 0.01% to 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The composition contains approximately 0.01% to 1.0% by weight of retinol, based on the total weight of the composition.

[0105] Preferably, a method for increasing CRABP2 or AQP3 expression in skin cells includes topical application of a skincare composition, the skincare composition is a. Approximately 0.01% to 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The composition contains approximately 0.01% to approximately 1.0% by weight of retinol, based on the total weight of the composition. Myrrh resin extract is obtained by extracting the resin of plants of the genus Commiphora with an organic solvent.

[0106] Preferably, a method for increasing CRABP2 or AQP3 expression in skin cells includes topical application of a skincare composition, the skincare composition is a. Approximately 0.01% to 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The composition contains approximately 0.01% to approximately 1.0% by weight of retinol, based on the total weight of the composition. Myrrh resin extract is obtained by extracting the resin of plants of the genus Commiphora with an organic solvent. The ratio of myrrh resin extract to retinol is approximately 10:1 to approximately 1:5, preferably approximately 10:1 to approximately 1:2, preferably approximately 5:1 to approximately 1:1, preferably approximately 2.5:1 to approximately 1:1; preferably approximately 1.25:1.

[0107] In another embodiment, the present invention may relate to a method for enhancing the effects of retinol in a skincare composition, comprising the addition of myrrh resin extract to the composition, wherein the amount of myrrh resin extract is about 0.001% to about 10% of the total weight of the composition.

[0108] Preferably, the myrrh resin extract is present in an amount ranging from about 0.001% to about 1% of the total weight of the composition, or from about 0.001% to about 0.5% of the total weight of the composition, or from about 0.001% to about 0.2% of the total weight of the composition, or from about 0.001% to about 0.1% of the total weight of the composition, or from about 0.001% to about 0.01% of the total weight of the composition, or from about 0.00001% to about 2% of the total weight of the composition, or from about 0.0001% to about 2% of the total weight of the composition, or from about 0.001% to about 2% of the total weight of the composition, or from about 0.01% to about 1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount exceeding 0.005% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount greater than 0.01% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.5% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.2% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.1% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.05% of the total weight of the composition. Preferably, the myrrh resin extract is present in an amount less than 0.02% of the total weight of the composition.

[0109] Preferably, retinol is present in an amount ranging from about 0.001% to about 5%, or from about 0.001% to about 1%, or from about 0.001% to about 0.5%. Preferably, retinol is present in an amount greater than 0.005% of the total weight of the composition. Preferably, retinol is present in an amount greater than 0.01% of the total weight of the composition. Preferably, retinol is present in an amount greater than 0.05% of the total weight of the composition. Preferably, retinol is present in an amount greater than 0.1% of the total weight of the composition. Preferably, retinol is present in an amount less than 5% of the total weight of the composition. Preferably, retinol is present in an amount less than 1% of the total weight of the composition. Preferably, retinol is present in an amount less than 0.5% of the total weight of the composition.

[0110] Preferably, the ratio of myrrh resin extract to retinol is about 10:1 to about 1:5. Preferably, about 10:1 to about 1:2, preferably about 5:1 to about 1:1, preferably about 2.5:1 to about 1:1; preferably about 1.25:1.

[0111] The present invention will be better understood through the following embodiments, which are essentially illustrative and not intended to limit to any specific combination of elements. [Examples]

[0112] method EpiDerm Full Thickness Skin 3D Equivalents (EFT-400) were purchased from MatTek Corporation (Ashland, MA 01721, lot number 34272, kit N and kit P). Upon receipt, the skin equivalents were transferred to new 6-well plates (MatTek Corporation, Ashland, MA 01721) containing 2.5 mL of EFT-400 medium (MatTek Corporation, Ashland, MA) and equilibrated overnight.

[0113] The following day, skin equivalents were topically treated with 4 μL of the following: vehicle, 0.04% retinol, 0.04% retinol + 0.05% myrrh resin extract, or 0.04% retinol + 0.1% myrrh resin extract.

[0114] Propylene glycol / ethanol (PgETOH) (3:7) (w / w) was used as the vehicle. Retinol (BASF 50C) was diluted in PgETOH to a 0.4% stock concentration. The 0.4% retinol was further diluted to prepare 0.08% retinol. Since retinol is 50% active, the percentage of active retinol in the final treatment solution was 0.04% retinol. Myrrh resin extract (Firmenich) was weighed to 16.1 mg and diluted in 1.6 mL of PgETOH for a 1% myrrh resin extract stock solution.

[0115] To prepare a 0.04% retinol + 0.1% myrrh resin extract treatment solution, combine 100 μL of 0.4% retinol stock solution, 50 μL of 1% myrrh resin extract stock solution, and 350 μL of PgETOH. To prepare a 0.04% retinol + 0.05% myrrh resin extract treatment solution, combine 100 μL of 0.4% retinol stock solution, 25 μL of 1% myrrh resin extract stock solution, and 375 μL of PgETOH.

[0116] After 24 hours of treatment, the EFT-400 skin equivalent was excised from the insert and dissolved in 600 μL of lysis buffer consisting of 100 parts of RLT buffer (RNA purification kit, trademark RNEASY Mini kit, Qiagen, Valencia, CA) and 1 part of 2-mercaptoethanol in a reinforced tube with a screw cap and O-ring seal. Tissue grinding beads (trademark PRECELLYS CKMix50, Bertin Corp, Rockville, MD) were also added to the tube. The tube was shaken for 4 cycles at 7200 RPM for 30 seconds each, with a 40-second pause between cycles. RNA was extracted from the solution using the RNEASY Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions, and the RNA was eluted in 30 μL of RNase-free water. Reverse transcription (RT) was performed using the Invitrogen SuperScript IV ViLo kit (ThermoFisher Scientific, Bridgewater, NJ).

[0117] Gene expression was performed using gene expression assays sold under the brand name TAQMAN for cellular retinoic acid-binding protein 2 (CRABP2), aquaporin-3 (AQP3), interleukin-8 (IL8), and polymerase (RNA) II polypeptide A (POLR2A), as well as using the Fast Advanced Master Mix (ThermoFisher Scientific, Bridgewater, NJ), also sold under the brand name TAQMAN. qPCR analysis was performed using the TAQMAN Fast Advanced Master Mix on a real-time PCR system sold under the brand name QUANTSTUDIO 7 Flex System (ThermoFisher Scientific, Bridgewater, NJ).

[0118] The expression of these genes was normalized against the expression of the human POLR2A "housekeeping" gene. Because this "housekeeping" gene is invariant in expression across tissues, cells, and experimental procedures, it is widely used as a control for normalizing specific gene expression levels. The normalization method involves measuring the expression of an internal reference gene or the "housekeeping" gene, taking into account potential errors in RNA / cDNA loading and variations in reverse transcription efficiency.

[0119] The magnification change was calculated by comparing it with the vehicle control, and a two-sided, two-sample Student's t-test (Microsoft Office Excel 2007, Microsoft, Redmond, WA, USA) was performed.

[0120] The results are shown in Tables 1-3 as the average magnification change relative to the vehicle.

[0121] "Untreated" means that the gene expression assay was performed using a vehicle:propylene glycol / ethanol (PgETOH) that does not contain retinol or myrrh resin extract.

[0122] result:

[0123] [Table 1]

[0124] [Table 2]

[0125] [Table 3]

[0126] Conclusion: CRABP2 is well-established in the literature as a highly sensitive marker for the biological activity and potency of retinoids (Elder JT, Cromie MA, Griffiths CEM, Chambon P, Voorhees JJ. Stimulus-selective induction of CRABP-II mRNA: A marker for retinoic acid action in human skin. J Invest Dermatol. 1993;100(4)), and is associated with the conferral of anti-aging benefits for the skin (Bielli A, Scioli MG, D'Amico F, Tarquini C, Agostinelli S, Costanza G, Doldo E, Campione E, Passeri D, Coniglione F, Orlandi A. Cellular retinoic acid binding protein-II expression and its potential role in skin aging. Aging (Albany NY). 2019 Mar 18;11(6):1619-1632).

[0127] Surprisingly, the combination of 0.04% retinol and 0.05% myrrh resin extract showed significantly higher CRABP2 induction than retinol alone.

[0128] This data indicates that the combination of retinol and myrrh resin extract results in increased retinol-like properties and therefore anti-aging benefits. Each biomarker is associated with anti-aging benefits, and such additional benefits from the combination are highly advantageous.

[0129] Alongside the increase in CRABP2 induction, no increase in IL8 was observed in most combinations. Retinoids are known to cause irritation and stinging upon application, which is considered IL8 induction in vitro. Our results show that the combinations resulted in increased retinol-like activity without the associated inflammatory side effects.

[0130] Aquaporin 3 (AQP3) is a passive water transporter involved in skin hydration and important for maintaining water homeostasis in the skin. AQP3 is indirectly induced by retinol. The above results demonstrate that the combination of retinol and myrrh resin extract shows a significantly increased induction of AQP3 compared to retinol alone. Our results show that this combination exhibits an increased benefit in skin hydration.

[0131] It has been observed that the active dose of compounds that provide in vitro benefits, such as myrrh resin extract or retinol, may generally require a 10-fold increase in vivo to exhibit similar benefits.

[0132] [Implementation Method] (1) A skincare composition, a. Myrrh resin extract, b. A skincare composition containing retinoids. (2) The skincare composition according to Embodiment 1, wherein the myrrh resin extract is produced from the resin of a plant of the genus Commiphora. (3) The skincare composition according to Embodiment 2, wherein the myrrh resin extract is obtained by extraction with an organic solvent. (4) The skincare composition according to Embodiment 1, wherein the myrrh resin extract is present in an amount ranging from about 0.000001% to about 10% of the total weight of the composition. (5) The skincare composition according to Embodiment 1, wherein the retinoid is selected from the group consisting of retinol, retinal, retinol ester, retinoic acid, derivatives thereof, and combinations thereof.

[0133] (6) The skin care composition according to Embodiment 1, wherein the retinoid is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition. (7) The skincare composition according to Embodiment 1, wherein the ratio of the myrrh resin to the retinoid is about 100:1 to about 1:10. (8) The skincare composition according to Embodiment 1, further comprising a component selected from the group consisting of surfactants, chelating agents, emollients, moisturizers, conditioners, preservatives, emulsions, fragrances, and combinations of two or more thereof. (9) The skincare composition according to Embodiment 1, wherein the composition is in the form of a solution, suspension, emulsion, lotion, cream, serum, gel, stick, spray, ointment, liquid cleanser, bar soap, shampoo, hair conditioner, paste, foam, powder, mousse, shaving cream, hydrogel, or film-forming product. (10) a. Myrrh resin extract in an amount of about 0.01% to about 2.0% by weight relative to the total weight of the composition, b. The skincare composition according to Embodiment 1, comprising about 0.01% to about 1.0% by weight of retinol based on the total weight of the composition.

[0134] (11) A method of skin treatment comprising applying the composition described in Embodiment 1 topically to the skin. (12) A method for treating signs of skin aging, comprising topically applying the skincare composition described in Embodiment 1 to skin that is in need of treatment for skin aging. (13) A method for enhancing the anti-aging effect of retinoids in the skin layer of skin where it is needed, comprising topically applying the skincare composition described in Embodiment 1 to the skin. (14) A method for increasing CRABP2 or AQP3 expression in skin cells, comprising topically applying the skincare composition described in Embodiment 1 to the skin. (15) The method according to Embodiment 14, wherein the retinoid is selected from the group consisting of retinol, retinal, retinol ester, retinoic acid, derivatives thereof, and combinations thereof.

[0135] (16) The method according to Embodiment 14, wherein the retinol is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition. (17) The method according to Embodiment 14, wherein the myrrh resin extract is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition. (18) The skincare composition which is applied topically to the skin, a. Approximately 0.01% to approximately 2.0% by weight of myrrh resin extract relative to the total weight of the composition, b. The method according to Embodiment 14, comprising about 0.01% to about 1.0% by weight of retinol based on the total weight of the composition.

Claims

1. A skincare composition, a. Myrrh resin extract, b. A skincare composition comprising a retinoid.

2. The skincare composition according to claim 1, wherein the myrrh resin extract is produced from the resin of a plant of the genus Commiphora.

3. The skincare composition according to claim 2, wherein the myrrh resin extract is obtained by extraction with an organic solvent.

4. The skincare composition according to claim 1, wherein the myrrh resin extract is present in an amount ranging from about 0.000001% to about 10% of the total weight of the composition.

5. The skincare composition according to claim 1, wherein the retinoid is selected from the group consisting of retinol, retinal, retinol ester, retinoic acid, derivatives thereof, and combinations thereof.

6. The skincare composition according to claim 1, wherein the retinoid is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

7. The skincare composition according to claim 1, wherein the ratio of the myrrh resin to the retinoid is about 100:1 to about 1:

10.

8. The skincare composition according to claim 1, further comprising a component selected from the group consisting of surfactants, chelating agents, emollients, moisturizers, conditioners, preservatives, emulsions, fragrances, and combinations of two or more thereof.

9. The skincare composition according to claim 1, wherein the composition is in the form of a solution, suspension, emulsion, lotion, cream, serum, gel, stick, spray, ointment, liquid cleanser, bar soap, shampoo, hair conditioner, paste, foam, powder, mousse, shaving cream, hydrogel, or film-forming product.

10. a. Myrrh resin extract in an amount of about 0.01% to about 2.0% by weight relative to the total weight of the composition, b. The skincare composition according to claim 1, comprising about 0.01% to about 1.0% by weight of retinol based on the total weight of the composition.

11. A method of skin treatment comprising applying the composition described in claim 1 topically to the skin.

12. A method for treating signs of skin aging, comprising applying the skincare composition described in claim 1 topically to skin requiring treatment for skin aging.

13. A method for enhancing the anti-aging effect of retinoids in the skin layer of skin where it is needed, comprising topically applying the skincare composition according to claim 1 to the skin.

14. A method for increasing CRABP2 or AQP3 expression in skin cells, comprising applying the skincare composition described in claim 1 topically to the skin.

15. The method according to claim 14, wherein the retinoid is selected from the group consisting of retinol, retinal, retinol ester, retinoic acid, derivatives thereof, and combinations thereof.

16. The method according to claim 14, wherein the retinol is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

17. The method according to claim 14, wherein the myrrh resin extract is present in an amount ranging from about 0.001% to about 10% of the total weight of the composition.

18. The skincare composition, which is applied locally to the skin, a. Myrrh resin extract in an amount of about 0.01% to about 2.0% by weight relative to the total weight of the composition, b. The method according to claim 14, comprising about 0.01% to about 1.0% by weight of retinol based on the total weight of the composition.

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