Highly efficient method for producing genetically modified cells
JP7632868B2Active Publication Date: 2025-02-19SHINSHU UNIVERSITY
Patent Information
- Application Number
- JP2020552621
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2018-10-26
- Filing Date
- 2019-10-25
- Publication Date
- 2025-02-19
- Estimated Expiration
- 2039-10-25
AI Technical Summary
Benefits of technology
【0012】 本発明の遺伝子改変細胞の作製方法は、従来の作製方法と比較して、極めて簡便な工程で、CAR発現細胞の増大を可能とし、さらに、本方法で作製されたCAR-T細胞は従来の作製方法と比較して同等以上の有効性を示す。
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Abstract
We aim to improve the efficiency of methods for producing chimeric antigen receptor (CAR)-expressing cells. The present invention provides a method for producing genetically modified mammalian cells, comprising the steps of: a) introducing a polynucleotide encoding a chimeric antigen receptor (CAR) protein into a cell population containing T cells derived from a mammal by transposon method to obtain a genetically modified cell population; b) preparing an endogenous cell population derived from the mammal that expresses a protein that binds to the CAR; and c) co-culturing the genetically modified cell population of step a) and the endogenous cell population of step b).
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Citation Information
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