Sap flow activator for fruit trees

Abstract

An aspect of the present invention relates to a sap-flow activator for fruit trees which contains humic acid as an active ingredient.

Description

[Technical field]

[0001] The present invention relates to a sap flow activator for fruit trees. [Background technology]

[0002] Sap flow refers to the flow of liquid (sap) within a plant. By measuring sap flow, for example, the water transpiration rate of the plant can be evaluated. Various methods for measuring sap flow have been proposed in the past (Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent No. 6007042 Summary of the Invention [Problem to be solved by the invention]

[0004] Although various sap flow measuring devices and methods have been reported, specific means for activating sap flow have not been known so far. The object of the present invention is to provide a novel sap flow activator for fruit trees. [Means for solving the problem]

[0005] The present invention relates to the following inventions. [1] A sap flow activator for fruit trees that contains humic acid as its active ingredient. [2] The sap flow activator for fruit trees described in [1], having a melanic index of 2.0 or more. [3] The sap flow activator for fruit trees according to [1] or [2], wherein the mass average molecular weight of the humic acid is 100 to 6,000. [4] The sap flow activator for fruit trees described in any of [1] to [3], wherein the active ingredient is a humic acid extract, and the total organic carbon concentration of the humic acid extract is 15,000 mg / L or more. [5] The sap flow activator for fruit trees described in any one of [1] to [4], wherein the humic acid is derived from lignite. [6] A method for activating sap flow in fruit trees, comprising applying humic acid to the fruit trees. Effect of the Invention

[0006] According to the present invention, a novel sap flow activator for fruit trees can be provided. [Brief description of the drawings]

[0007] [Figure 1] 1 is a graph showing the results of measuring the number of fruits per tree to which humic acid was applied. [Diagram 2] Graph (A) shows the results of measuring the total number of fruits harvested from fruit trees to which humic acid was applied, graph (B) shows the results of measuring the total weight (kg) of fruit harvested from fruit trees to which humic acid was applied, graph (C) shows the results of measuring the weight (kg) per fruit harvested from fruit trees to which humic acid was applied, and graph (D) shows the results of measuring the sugar content (Brix) of fruit harvested from fruit trees to which humic acid was applied. [Diagram 3] 1 is a graph showing solar irradiance during a test period. [Figure 4] 1 is a graph showing temperature, humidity, and saturation deficit during a test period. [Diagram 5] 1 is a graph showing the results of sap flow measurements over the test period. [Figure 6] 1 is a graph showing measurements of sap flow per leaf area over the test period. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] Hereinafter, an embodiment of the present invention will be described in detail. However, the present invention is not limited to the following embodiment. In this embodiment, mass may be referred to as weight.

[0009] In this specification, a numerical range indicated using "~" indicates a range including the numerical values ​​described before and after "~" as the minimum and maximum values, respectively. Unless specifically stated otherwise, the units of the numerical values ​​described before and after "~" are the same. In the numerical ranges described in stages in this specification, the upper limit or lower limit of a certain numerical range may be replaced by the upper limit or lower limit of a numerical range of another stage. In the numerical ranges described in this specification, the upper limit or lower limit of the numerical range may be replaced by a value shown in the examples. The upper limit and lower limit values ​​described individually can be combined in any combination.

[0010] The sap flow activator for fruit trees according to this embodiment contains humic acid as an active ingredient. Activating sap flow means stimulating the uptake of nutrients and water within the fruit tree.

[0011] Fruit trees are herbaceous plants and woody plants whose fruits are edible. Fruit trees may be deciduous or evergreen. Fruit trees may be, for example, fruit trees of the Rosaceae, Ebenaceae, Mulberry, Pomegranate, Ericaceae, Rosaceae, Vitaceae, Fagaceae, Actinidiaceae, or Rutaceae. Fruit trees of the Rosaceae family include fruit trees of the Prunus, Prunus, Pyrus, Loquat, or Malus genus. Specifically, fruit trees of the Rosaceae family may be, for example, pears or grapes. Fruit trees of the Rutaceae family include fruit trees of the Citrus, Fork, or Trifoliate orange genus. Specifically, fruit trees of the Rutaceae family may be, for example, mandarin oranges. An example of a fruit tree is Le Lectier, which is a variety of European pear of the Pyrus genus of the Rosaceae family.

[0012] In the present specification, the term "humic acid" includes humic acid and fulvic acid. The humic acid includes one or more selected from the group consisting of humic acid and humate salts.

[0013] Examples of humic acids include natural humic acids that are naturally produced in peat, weathered coal, etc., artificial humic acids that are artificially produced by nitric acid oxidation of lignite, etc., and humate salts obtained by neutralizing natural or artificial humic acids with alkaline substances such as sodium, potassium, ammonia, calcium, magnesium, etc. Examples of humic acids include fulvic acid, humic acid, nitrohumic acid, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium and magnesium nitrohumate, potassium humate, potassium nitrohumate, etc.

[0014] The active ingredient may be a humic acid extract, which may be an extract obtained by extracting nitric acid oxide from young charcoal with an extraction solvent containing water and, if necessary, an alkali.

[0015] Young coal is defined as coal with a lower carbon content than bituminous coal, etc., and has a carbon content of 83 mass% or less. Examples of young coal include peat, lignite, brown coal, and subbituminous coal. Young coal may be used alone or in combination of two or more types. Humic acid may be derived from brown coal in terms of the sap flow activation effect.

[0016] Nitric acid oxide of young coal is obtained by oxidatively decomposing young coal with nitric acid. Concentrated nitric acid is preferable as nitric acid. In terms of safety and reactivity, it is preferable to use nitric acid with a concentration of 40 to 60 mass%. The amount of nitric acid (HNO3) to be blended during oxidative decomposition may be 10 parts by mass or more, or 20 parts by mass or more, relative to 20 parts by mass of young coal, and may be 300 parts by mass or less, 250 parts by mass or less, 200 parts by mass or less, 150 parts by mass or less, 100 parts by mass or less, 50 parts by mass or less, 36 parts by mass or less, or 20 parts by mass or less. The amount of nitric acid (HNO3) to be blended may be 10 to 20 parts by mass, or 20 to 36 parts by mass, relative to 20 parts by mass of young coal. Here, the amount of nitric acid to be blended is a value converted to 100% nitric acid (100% HNO3).

[0017] The temperature during the oxidative decomposition may be, for example, 70 to 95° C. As a starter for the oxidation reaction, heating to 70 to 95° C. in a water bath or the like tends to facilitate the oxidation reaction to proceed quickly. The reaction time may be, for example, 20 minutes or more, 0.5 hours or more, or 1 hour or more, and may be 6 hours or less, 4 hours or less, or 1 hour or less.

[0018] The humic acid extract is obtained as a liquid by, for example, stirring nitric acid oxide of young coal (hereinafter referred to as crude humic acid) with an extraction solvent containing water and alkali, followed by a solid-liquid separation process.

[0019] Examples of the alkali include hydroxides and ammonia. Examples of the hydroxides include hydroxides of alkali metals and ammonium hydroxide. The hydroxides are preferably hydroxides of alkali metals. Examples of the hydroxides of alkali metals include potassium hydroxide and sodium hydroxide. The hydroxides are preferably one or more of potassium hydroxide, sodium hydroxide, and ammonium hydroxide (aqueous ammonia). The pH of the extraction solvent may be 0.5 to 7.0, 0.5 to 4.0, or 1.0 to 3.0.

[0020] The temperature (extraction temperature) when the humic acid crude product is extracted with the extraction solvent may be, for example, 40 to 90° C., from the viewpoint of further suppressing freezing and deterioration of the extract. The time (extraction time) for extracting the humic acid crude product with the extraction solvent may be, for example, 0.5 hours or more, 24 hours or less, or 1 hour or less.

[0021] The amount of extraction solvent relative to the amount of raw material young coal used to prepare the humic acid crude product is defined as the solid-liquid ratio. For example, when 100 g (100 mL) of extraction solvent (water) is added to a crude product prepared from 20 g of young coal, the solid-liquid ratio (extraction solvent / young coal) is 5. The solid-liquid ratio may be 3 or more, 4 or more, 5 or more, 6 or more, 7 or more, 8 or more, 9 or more, or 10 or more, and may be 15 or less, 13 or less, 11 or less, 9 or less, 7 or less, or 6 or less. The solid-liquid ratio can be adjusted by adding water. The solid-liquid ratio may be adjusted to the desired solid-liquid ratio after adjusting the pH. The method of solid-liquid separation may be centrifugation, filter press, etc.

[0022] The total organic carbon concentration (TOC) of the humic acid extract may be at least 15,000 mg / L, at least 15,300 mg / L, at least 15,500 mg / L, at least 16,000 mg / L, at least 16,500 mg / L, at least 17,000 mg / L, at least 17,500 mg / L, at least 18,000 mg / L, at least 18,500 mg / L, at least 19,000 mg / L, at least 19,500 mg / L, at least 20,000 mg / L, or at least 20,500 mg / L. The TOC of the humic acid extract may be 75,000 mg / L or less, 70,000 mg / L or less, 65,000 mg / L or less, 60,000 mg / L or less, 55,000 mg / L or less, 50,000 mg / L or less, 45,000 mg / L or less, 40,000 mg / L or less, 35,000 mg / L or less, 30,000 mg / L or less, 25,000 mg / L or less, 24,000 mg / L or less, 23,000 mg / L or less, or 22,000 mg / L or less.

[0023] The method for measuring the TOC of humic acid extract is defined as follows: The TOC of the humic acid extract is measured by centrifuging the humic acid extract at 3,000 x g and then measuring the supernatant using a total organic carbon meter (TOC-L, manufactured by Shimadzu Corporation) with a combustion catalytic oxidation method. If it contains non-humic substances such as urea, a fertilizer component, the fractions (humic acid fraction and fulvic acid fraction) are separated according to the International Humic Substances Society method (Fujitake, Humic Substances Research Vol. 3, p. 1-9) and quantified using the above method, and the TOC of the humic acid extract is measured.

[0024] Humic acids can be classified into A-type, B-type, Rp-type and P-type. From the viewpoint of being more effective on fruit trees, the humic acid may be Rp-type or P-type humic acid (Rp·P-type humic acid). Classification of humic acids can be easily performed by the melanic index (MI) described later. Humic acids with an MI of 2.0 or more are classified as Rp·P-type.

[0025] In order to make it more effective on fruit trees, the melanic index (MI) of humic acid may be, for example, 1.5 or more, 2.0 or more, 2.2 or more, 2.5 or more, 3.0 or more, or 3.5 or more, and may be 6.5 or less, 6.0 or less, 5.5 or less, 5.0 or less, 4.5 or less, 4.0 or less, 3.5 or less, or 3.0 or less.

[0026] MI is an index used to classify humic acids, and is the absorbance (A) at 450 nm in the absorption spectrum of sodium hydroxide extract. 450 ) and absorbance at a wavelength of 520 nm (A 520 ) and the ratio (A 450 / A 520 (Kyōichi Kumada, Chemistry of Soil Organic Matter, 2nd Edition, Academic Press Center (1981), Japanese Journal of Soil and Plant Nutrition, Vol. 71, No. 1, p. 82-85 (2000)).

[0027] More specifically, the MI is calculated by the following method. The sample is crushed into 250 μm sieve using a mortar and a 250 μm sieve. Approximately 10 g of the crushed sample is weighed in a weighing bottle with a known mass. The weighing bottle is left in a dryer maintained at 105°C for approximately 12 hours, and then returned to room temperature of 20°C in a desiccator and weighed again. The weight loss is regarded as moisture, and the moisture content of the sample is calculated. Next, 0.10 g of the 250 μm sieve, equivalent to the dry mass, and 45 ml of 0.5 mol / L sodium hydroxide solution are placed in a 50 ml centrifuge tube, and the tube is shaken at 250 rpm for approximately 1 hour at room temperature of 20°C, and then centrifuged at 3,000×g for approximately 10 minutes, and the supernatant is filtered through Advantec No. 5C filter paper. The absorbance of the filtrate at 450 nm and 520 nm is measured using distilled water as a blank. In this case, if the absorbance at 450 nm is 1.0 or more, add 0.1 mol / L sodium hydroxide aqueous solution to adjust the absorbance to 0.8 or more but less than 1.0, and then measure the absorbance at 520 nm. The ratio of the absorbance at 450 nm to the absorbance at 520 nm (absorbance at 450 nm / absorbance at 520 nm) is calculated and used as the MI.

[0028] The mass average molecular weight of the humic acid may be 100 to 6,000. The lower limit of the mass average molecular weight of the humic acid may be, for example, 200 or more, 300 or more, 400 or more, 500 or more, 600 or more, 700 or more, 800 or more, 900 or more, or 1,000 or more. The upper limit of the mass average molecular weight of the humic acid may be, for example, 5,500 or less, 5,000 or less, 4,500 or less, 4,000 or less, 3,500 or less, 3,000 or less, 2,500 or less, 2,000 or less, 1,500 or less, 1,200 or less, or 1,000 or less.

[0029] The mass average molecular weight of humic acid is measured by HPSEC method (GPC method) using Alliance HPLC System manufactured by Waters. The column is SB-803HQ manufactured by Showa Denko K.K., the standard sample is sodium polystyrene sulfonate, and the detection wavelength is 260 nm. The mobile phase is 10 mmol / L sodium phosphate buffer containing 25 mass% acetonitrile, the flow rate is 0.8 ml / min, and the column temperature is 40°C (column oven setting value).

[0030] ΔlogK(log(A 400 / A 600 From the viewpoint of being more effective on fruit trees, ΔlogK(log(A)) may be, for example, 0.7 or more, or 0.8 or more, and 2.0 or less, or 1.8 or less. 400 / A 600 The higher the A is, the fewer carbon-carbon double bonds there are, and the less carbonization there tends to be. 400 means the absorbance at a wavelength of 400 nm, and A 600 means absorbance at a wavelength of 600 nm. ΔlogK can be measured by the method described in the Humic Substances Analysis Handbook, 2nd Edition, p.86-88 and Environmental Humic Substances: Their Characteristics and Research Methods (Sankyo Publishing), p.19. Specifically, ΔlogK of humic acid can be measured using a UV-1850 ultraviolet-visible spectrophotometer manufactured by Shimadzu Corporation.

[0031] Humic acids with an MI of 2.0 or more and a ΔlogK of 0.7 or more tend to be more effective in plants.

[0032] The formulation of the sap flow activator for fruit trees may be, for example, a liquid or a powder. The powder can be obtained as a re-dissolvable powder by, for example, drying up the liquid sap flow activator for fruit trees by freeze-drying or the like.

[0033] The sap flow activator for fruit trees may consist of only humic acid, or may contain other components other than humic acid. The content of humic acid in the sap flow activator for fruit trees may be, for example, 5% by mass or more, 10% by mass or more, 20% by mass or more, 30% by mass or more, 40% by mass or more, 50% by mass or more, 60% by mass or more, 70% by mass or more, 80% by mass or more, 90% by mass or more, or 95% by mass or more, based on the total mass of the sap flow activator for fruit trees, and may be 100% by mass or less, 99% by mass or less, or 95% by mass or less.

[0034] Examples of other components include spreading agents, fertilizers, and plant activators. Examples of fertilizers include ammonium sulfate, potassium nitrate, and ammonium phosphate. Examples of plant activators include seaweed extracts and amino acids. Examples of amino acids include glycine, proline, and glutamic acid. The total content of other components may be, for example, 80% by mass or less, 60% by mass or less, 40% by mass or less, 30% by mass or less, 20% by mass or less, 10% by mass or less, 5% by mass or less, or 1% by mass or less, based on the total mass of the fruit tree sap flow activator, and may be more than 0% by mass, 1% by mass or more, or 5% by mass or more.

[0035] When the sap flow activator for fruit trees according to the present embodiment is applied, the sap flow of the fruit trees is activated compared to when the sap flow activator for fruit trees is not applied. For example, when the sap flow activator for fruit trees is applied, the average sap flow rate per leaf area can be increased by 1% or more or 3 to 25% compared to when the sap flow activator for fruit trees is not applied. The sap flow rate can be measured by the method described in the examples below.

[0036] By applying a sap flow activator for fruit trees, for example, it is possible to increase yield while maintaining quality, and to promote healthy growth of fruit trees and fruits.

[0037] The method for activating sap flow in fruit trees according to the present embodiment includes applying humic acid to the fruit trees. Methods for applying humic acid to fruit trees include soil drenching or soil incorporation, and foliar application.

[0038] The amount and period of application of humic acid are not particularly limited. In the case of soil application, 7,000 to 20,000 mg of total organic carbon can be applied once per fruit tree, or 0.1 to 1,000 mg / L of total organic carbon concentration can be applied 1 to 30 times per month, or about 0.1 to 100 mg / L of total organic carbon concentration can be applied daily. In the case of foliar application, 0.1 to 1,000 mg / L of total organic carbon concentration can be applied 1 to 12 times per month.

[0039] The present invention described above can also be understood as the use of humic acid to activate sap flow in fruit trees. As a specific embodiment in this aspect, the specific embodiment described in the above-mentioned sap flow activator for fruit trees can be applied.

[0040] The present invention described above can also be understood as a humic acid for use in activating sap flow in fruit trees. As a specific embodiment of this aspect, the specific embodiment described in the above-mentioned sap flow activator for fruit trees can be applied.

[0041] The present invention described above can also be understood as the use (application) of humic acid for the manufacture of a sap flow activator for fruit trees. As a specific embodiment in this aspect, the specific embodiment described in the above-mentioned sap flow activator for fruit trees can be applied. EXAMPLES

[0042] The present invention will be described in more detail below with reference to examples, although the present invention is not limited to the following examples.

[0043] <Preparation of humic acid materials> The humic acid material used was a material containing a humic acid extract prepared by the method described below.

[0044] In a draft, 500 g of lignite with a carbon content of 77% by mass was placed in a 1,000 ml beaker, and 625 g of nitric acid with a concentration of 48% by mass (60 parts by mass of 100% nitric acid per 100 parts by mass of young coal) was added. The oxidation reaction was carried out for 3 hours in a water bath at 80°C. The crude product containing humic acid obtained by this operation was subjected to the following extraction operation.

[0045] About 900 mL of 0.5 mol / L potassium hydroxide aqueous solution was added to 100 g of this crude product, and while monitoring with a pH meter, 1.0 mol / L potassium hydroxide aqueous solution was appropriately added to adjust the pH to 6.5. Water was added so that the solid-liquid ratio was 1:10 (solid-liquid ratio 10), and extraction was performed for 1 hour at 80°C. This extract was centrifuged at 3,000 x g, and the obtained supernatant was appropriately diluted, and the mass average molecular weight, total organic carbon concentration (TOC), melanic index (MI), and ΔlogK were measured.

[0046] The MI of the humic acid in the humic acid extract was 2.2. The total organic carbon concentration (TOC) of the humic acid extract was 34,000 mg / L. The mass average molecular weight of the humic acid in the humic acid extract was 4,300. The ΔlogK of the humic acid was 0.9.

[0047] [Mass average molecular weight] The mass average molecular weight of humic acid was measured by HPSEC method (GPC method) using Alliance HPLC System manufactured by Waters. The column was SB-803HQ manufactured by Showa Denko K.K., the standard sample was sodium polystyrene sulfonate, and the detection wavelength was 260 nm. The mobile phase was 10 mmol / L sodium phosphate buffer containing 25 mass% acetonitrile, the flow rate was 0.8 ml / min, and the column temperature was 40°C (column oven setting value).

[0048] [Total organic carbon concentration (TOC)] The TOC of the humic acid extract was measured using a total organic carbon meter (Shimadzu TOC-L) by the combustion catalytic oxidation method.

[0049] [Melanic Index (MI)] The sample was crushed into a 250 μm sieve using a mortar and a 250 μm sieve. Approximately 10 g of the crushed sample was weighed in a weighing bottle with a known mass. The weighing bottle was left in a dryer maintained at 105°C for approximately 12 hours, and then returned to room temperature in a desiccator and weighed again. The weight loss was considered to be moisture, and the moisture content of the sample was calculated. Next, 0.10 g of the 250 μm sieve in terms of dry mass equivalent and 45 ml of 0.5 mol / L sodium hydroxide solution were placed in a 50 ml centrifuge tube, and the tube was shaken at 250 rpm for approximately 1 hour at room temperature of 20°C, and then centrifuged at 3,000×g for approximately 10 minutes, and the supernatant was filtered through Advantec No. 5C filter paper. The absorbance of the filtrate at 450 nm and 520 nm was measured using distilled water as a blank. In this case, if the absorbance at 450 nm was 1.0 or more, 0.1 mol / L sodium hydroxide solution was added to adjust the absorbance to 0.8 or more and less than 1.0, and then the absorbance at 520 nm was measured. The ratio of the absorbance at 450 nm to the absorbance at 520 nm (absorbance at 450 nm / absorbance at 520 nm) was calculated and used as the MI.

[0050] [ΔlogK] The sample was crushed into a 250 μm sieve using a mortar and a 250 μm sieve. Approximately 10 g of the crushed sample was weighed in a weighing bottle with a known mass. The weighing bottle was left in a dryer maintained at 105° C. for approximately 12 hours, and then returned to room temperature in a desiccator and weighed again. The weight loss was regarded as moisture and the moisture content of the sample was calculated. Next, 0.10 g of the 250 μm sieve in terms of dry mass equivalent and 45 ml of 0.5 mol / L sodium hydroxide solution were placed in a 50 ml centrifuge tube, and the tube was shaken at 250 rpm for approximately 1 hour at room temperature of 20° C., and then centrifuged at 3,000×g for approximately 10 minutes, and the supernatant was filtered through Advantec No. 5C filter paper. The absorbance of the filtrate at 400 nm and 600 nm was measured using distilled water as a blank. In this case, if the absorbance at 400 nm was 1.0 or more, 0.1 mol / L sodium hydroxide solution was added to adjust the absorbance to 0.8 or more and less than 1.0, and then the absorbance at 600 nm was measured. The ratio of log (absorbance at 400 nm / absorbance at 600 nm) was calculated to obtain ΔlogK.

[0051] [Test Example 1: Cultivation test of Le Lectier] The cultivation test of Le Lectier was carried out under the following conditions. ·2-row cultivation Number of Le Lectier trees per acre: 9 -Distance between rows: approx. 3.5m ·Distance between trees: approx. 2m

[0052] The ridge where humic acid was applied was designated as the humic acid area. The second ridge from the humic acid area was designated as the control area where humic acid was not applied, so that the soil would not be strongly affected by the humic acid material due to seepage. In the humic acid area, 100 L of agricultural material diluted 200 times was applied per tree on June 30, 2020 (the start date of the test).

[0053] Humic acid was applied as follows. The test was carried out in an orchard in Niigata Prefecture, and the humic acid was applied by preparing a diluted humic acid solution in a tank and spraying a specified amount around the outside of the trees.

[0054] The trees already had fruits measuring about 5 cm in length. The trees will continue to bear new fruits. Tests were conducted on nine trees each in the humic acid and control areas.

[0055] Sap flow in the humic acid and control areas was measured using a sap flow sensor. Branches that grew directly upward from the main branch of each fruit tree and were roughly the same thickness were selected from each plant, and a sap flow sensor was attached to the selected branch. Sap flow was measured using the stem heat balance method. The sensor was covered with urethane to reduce the effects of rain, wind, etc. on the values.

[0056] Figure 1 shows the measurement results of the number of fruits per tree as of October 12, 2020, one week before harvest. As shown in Figure 1, the number of fruits per tree in the humic acid area increased by 31% compared to the control area.

[0057] Figures 2(A) to (D) show the total number of fruits harvested, total weight (kg), weight per fruit (kg), and Brix as of October 20, 2020. Brix measurement: N=3

[0058] As shown in Figures 2 (A) and (B), the total number and weight of fruit harvested increased in the humic acid area compared to the control area. As shown in Figures 2 (C) and (D), no significant changes were observed in the weight and sugar content per fruit. The total number of fruit harvested as of October 20, 2020, was lower than that as of October 12. This is thought to be due to poor quality fruit or fallen fruit.

[0059] The test results are summarized in Table 1. The lowest molecular height diameter (cm) refers to the diameter of the trunk just below the first branching point (1 cm). The lowest molecular height cross-sectional area (cm 2 ) refers to the area of ​​the trunk just below (1 cm) the first branching point. Number of branches (trees) refers to the number of branches per tree one week before the final harvest. Number of fruits (pieces / tree) refers to the number of fruits per tree out of nine trees in each plot one week before the final harvest. Fruit yield per unit area (pieces / cm 2 ) means the amount of fruit per molecular cross-sectional area on the tree one week before the final harvest. Number of leaves / branch means the number of leaves on the branch where sap flow was measured. Leaf area (cm 2 ) was measured by taking 10 or more pieces from each tree (100 pieces in the control area, 90 pieces in the humic acid area). The sugar content Brix (%) is shown as a result of N=3. The sugar content was measured using a sugar-acidity meter PAL-BX|ACID F5 manufactured by Atago Co., Ltd.

[0060] [Table 1]

[0061] It was confirmed that there was no significant difference between the individual fruit trees in the control and humic acid plots. As shown in Table 1, it was shown that the application of humic acid increased the number of fruits.

[0062] Figure 3 shows the solar irradiance from 00:00 on October 1, 2020 to 00:00 on October 5, 2020. Figure 4 shows the temperature, humidity, and saturation deficit from October 1, 2020 to October 5, 2020.

[0063] Figures 5 (A) and (B) show the results of sap flow measurements taken from 00:00 on October 1, 2020 to 00:00 on October 5, 2020. Figures 6 (A) and (B) show the results of sap flow measurements per leaf area taken from 00:00 on October 1, 2020 to 00:00 on October 5, 2020. Sap flow rate per leaf area is the sap flow rate per total leaf area of ​​the measured branch. The total leaf area of ​​the measured branches is calculated by "number of leaves / branch (pieces)" x "leaf area". The total leaf area of ​​the measured branches was 1,515 cm in the control area and 1,515 cm in the control area. 2 and 1480 cm in the humic acid area. 2 It was.

[0064] As shown in Figures 5(A) and (B) and Figures 6(A) and (B), the humic acid plots tended to have higher sap flow rates than the control plots.

Claims

1. A method for treating a soil irritation comprising the steps of: A sap flow activator for fruit trees having a melanic index of 2.0 or more.

2. A method for treating a soil ulcer comprising the steps of: The active ingredient is a humic acid extract, A sap flow activator for fruit trees, wherein the total organic carbon concentration of the humic acid extract is 15,000 mg / L or more.

3. The sap flow activator for fruit trees according to claim 1 or 2, wherein the mass average molecular weight of the humic acid is 100 to 6,000.

4. The sap flow activator for fruit trees according to claim 1 or 2, wherein the humic acid is derived from lignite.

5. 1. A method for stimulating sap flow in fruit trees, comprising: applying humic acid to said fruit tree; The method according to claim 1, wherein the humic acid has a melanic index of 2.0 or greater.

6. A method for activating sap flow in fruit trees, comprising the steps of: applying a humic acid extract to said fruit tree; The method, wherein the total organic carbon concentration of the humic acid extract is 15,000 mg / L or more.

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