Optimized CRISPR-Cas dual nickase system, methods and compositions for sequence engineering

The CRISPR complex with optimized Cas9 enzymes and enhanced guide sequences addresses the challenges of genome editing by achieving precise and efficient modifications in eukaryotic cells with reduced off-target effects.

JP7682975B2Active Publication Date: 2025-05-26THE BROAD INST INC +2
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Patent Information

Application Number
JP2023189768
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Priority Date
2013-12-12
Filing Date
2023-11-07
Publication Date
2025-05-26
Estimated Expiration
2034-06-10

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Abstract

To provide delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and / or activities of target sequences.SOLUTION: Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.SELECTED DRAWING: None
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Citation Information

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