Auxin degron system kit and use thereof

The auxin degron system kit addresses the permeability issues of auxin analogs in non-plant eukaryotic cells by using a mutant TIR1 family protein and auxin analogue complex, achieving efficient and controlled protein degradation.

JP7760117B2Active Publication Date: 2025-10-27INTER UNIV RES INST RES ORG OF INFORMATION & SYST +1
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Patent Information

Application Number
JP2021135637
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Filing Date
2021-08-23
Publication Date
2025-10-27
Estimated Expiration
2041-08-23

AI Technical Summary

Technical Problem

The existing auxin-inducible degron systems face challenges with impaired membrane permeability of auxin or auxin analogs due to the barrier function of the eggshell and blood-brain barrier, limiting precise control and efficiency of protein degradation in non-plant eukaryotic cells.

Method used

A kit comprising a mutant TIR1 family protein with a mutation in the auxin-binding site, an auxin analogue with affinity for the mutant TIR1 family protein, and a degradation tag that forms a complex with the mutant TIR1 family protein-auxin analogue, enhancing membrane permeability and enabling efficient protein degradation in non-plant eukaryotic cells.

Benefits of technology

The system allows for precise and efficient control of protein degradation in non-plant eukaryotic cells, overcoming the limitations of membrane permeability barriers and reducing the toxic effects of high auxin concentrations.

✦ Generated by Eureka AI based on patent content.

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Abstract

To provide auxin-degron system kits capable of efficiently controlling the degradation of a target protein.SOLUTION: The kit of the present invention is an auxin-degron system kit that controls the degradation of a target protein in a non-plant-derived eukaryotic cell, comprising: a first nucleic acid encoding a mutant-type TIR1 family protein having a mutation in the auxin binding site; an auxin analog having affinity for the mutant-type TIR1 family protein; and a second nucleic acid comprising at least a portion of an Aux / IAA family protein and encoding a degradation tag having affinity for the mutant-type TIR1 family protein-the auxin analog complex.SELECTED DRAWING: None
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Citation Information

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