Anti-aging gene expression promoter

Abstract

To provide a material that promotes the expression of LINC00942, particularly in lip-derived fibroblasts.SOLUTION: One or two or more Lamiales plant extracts selected from sage extract, perilla extract, lavender extract, rosemary extract, and common verbena extract, and Lagerstroemia speciosa extract are used as active ingredients of a LINC00942 expression promoter. The LINC00942 expression promoter can be blended in a composition for suppressing skin aging. In addition, one or two or more Lamiales plant extracts selected from sage extract, perilla extract, lavender extract, rosemary extract, and common verbena extract, and Lagerstroemia speciosa extract are contained in a lip cosmetic. The cosmetics are expected to be able to increase myofiber-constituting proteins of lips, and to realize lips that are three-dimensional, thick, and substantial.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an anti-aging gene expression promoter, a skin aging inhibitory composition containing the expression promoter, and a lip cosmetic containing the anti-aging gene expression promoter. [Background technology]

[0002] The cells that make up living organisms age with time. Cells are responsible for biological reactions and form and maintain the tissues and organs that carry out life activities, so cellular aging leads to the aging of the individual. Furthermore, senescent cells release factors that induce senescent-like responses in surrounding cells, accelerating the aging of tissues and organs. For this reason, it has been suggested that removing senescent cells can slow down the aging of individuals and tissues and improve existing aging symptoms (see, for example, Non-Patent Document 1).

[0003] Therefore, senescent cells are expected to be a target for anti-aging (see, for example, Non-Patent Document 2). However, there has been a challenge in that non-invasive removal of senescent cells is difficult to implement.

[0004] On the other hand, in recent years, non-coding RNA (ncRNA), which is RNA that does not code for proteins, has been reported as a biological component that controls cellular senescence (for example, Non-Patent Document 3). (See reference). Among these ncRNAs, long-chain ncRNAs (LncRNAs), which have more than 200 base pairs, are known, and it is estimated that there are about 8200 types of LncRNAs (see, for example, Non-Patent Document 4). Regarding LncRNAs, in recent years, skin samples have been taken from young and elderly donors, and their expression levels have been compared, reporting that the amount of several LncRNAs changes with age (see, for example, Non-Patent Document 5).

[0005] LncRNAs such as NESPAS, FLJ46906, and HOTAIR, whose expression increases with age, and LncRNAs such as SNHG5, LOC100292680 (also known as "LINC00942," which represent the same LncRNA), and IPW, whose expression decreases with age, are particularly powerful indicators for determining the inhibitory effect on cellular senescence. It has been reported that the effect of suppressing skin aging can be determined using this as an indicator (see, for example, Patent Documents 1 and 2). Furthermore, LINC00942 was identified as an anti-aging gene, and components that promote its expression were found. Anti-aging cosmetics containing these ingredients have also been proposed (Patent Document 3). [Prior art documents] [Patent Documents]

[0006] [Patent Document 1] Japanese Patent Publication No. 2015-130857 [Patent Document 2] Japanese Patent Publication No. 2017-112892 [Patent Document 3] Japanese Patent Application No. 2020-012419 [Non-patent literature]

[0007] [Non-Patent Document 1] Baker DJ. et al., Nature. (2011), 479, 232-236 [Non-Patent Document 2] Naylor RM. et al., Clin Pharmacol Ther. (2013) 93, 105-116 [Non-Patent Document 3] Abdelmohsen K. et al., Wiley Interdiscip Rev RNA. (2015) 6, 615-629 [Non-Patent Document 4] Cabili MN. et al., Genes Dev. (2011), 25, 1915-1927

Non-Patent Document 5

Summary of the Invention

Problems to be Solved by the Invention

[0008] To provide a material that promotes the expression of LINC00942, particularly in fibroblasts derived from the lip. This is the problem.

Means for Solving the Problems

[0009] The inventors of the present invention conducted investigations on various materials in search of a new skin aging inhibitory material. As a result, when a extract of a Lamiaceae plant such as sage and a Paeonia suffruticosa extract are combined, the expression of LINC00942, which is a lncRNA, is promoted (or enhanced), and it was found to be effective as a skin aging inhibitory material. Furthermore, such expression promotion was observed in fibroblasts derived from the lip, and based on the finding of its usefulness as a component to be incorporated into lip cosmetics for skin aging inhibition, the present invention was completed. Furthermore, the inventors of the present invention found that the expression of muscle fiber constituent proteins in skeletal muscle cells adjacent to lip-derived fibroblasts is increased, and that lip-derived fibroblasts with enhanced expression of LINC00942 were also found to further increase the muscle fiber constituent proteins in adjacent skeletal muscle cells. Based on such findings, a combination of an extract of a Lamiaceae plant such as sage, which can increase the expression of LINC00942 in lip-derived fibroblasts, and a Paeonia suffruticosa extract led to the idea that it can increase the muscle fiber constituent proteins of the lip, and thus completed the invention of a new lip cosmetic.

[0010] That is, the present invention is as follows. [1] An expression promoter for LINC00942 containing one or more extracts of Lamiales plants selected from sage extract, perilla extract, lavender extract, rosemary extract, and verbena extract, as well as crape myrtle extract. [2] Promote expression of LINC00942 in lip-derived fibroblasts, as described in [1]. Agent. [3] The expression promoter according to [1] or [2], wherein the dry weight ratio of the content of Lamiales plant extract to Lagerstroemia indica extract is 20:1 to 100:1. [4] A composition for inhibiting skin aging, containing one or more extracts of Lamiales plants selected from sage extract, perilla extract, lavender extract, rosemary extract, and verbena extract, and crape myrtle extract. [5] The composition according to [4], wherein the skin aging suppression is one or more selected from prevention of decreased skin elasticity, prevention of wrinkles, prevention of sagging, prevention of blemish formation, prevention of dullness, and prevention of pore enlargement. [6] The composition according to [4] or [5], which is a cosmetic for the lips. [7] A lip cosmetic containing one or more extracts of Lamiales plants selected from sage extract, perilla extract, lavender extract, rosemary extract and verbena extract, and crape myrtle extract. [8] The cosmetic described in [7], used to increase the muscle fiber constituent proteins of the lips. [Effects of the Invention]

[0011] According to the present invention, a new skin has the effect of promoting the expression of the anti-aging gene LINC00942. We can provide materials that inhibit aging. Furthermore, according to the present invention, by increasing the expression of LINC00942 in lip-derived fibroblasts, Because the muscle fiber constituent proteins in the adjacent skeletal muscle cells increase, it is expected that this product will provide a lip cosmetic that gives lips a three-dimensional, thick, and voluminous appearance, thereby creating youthful or attractive lips. [Brief explanation of the drawing]

[0012] [Figure 1] A graph showing the expression level of LINC00942 in lip-derived fibroblasts cultured with the test sample. [Figure 2] Fluorescent staining images of muscle fiber constituent protein (MYH2) in skeletal muscle cells treated with culture supernatant of lip-derived fibroblasts (green: muscle fiber constituent protein, blue: cell nucleus). [Figure 3] Fluorescence staining image of muscle fiber constituent protein (MYH7) in skeletal muscle cells to which the culture supernatant of lip-derived fibroblasts has been added (green: muscle fiber constituent protein, blue: cell nucleus). [Figure 4] A graph showing the expression levels of the muscle fiber constituent protein (MYH2) gene in skeletal muscle cells to which the culture supernatant of lip-derived fibroblasts has been added. [Figure 5] A graph showing the expression levels of the muscle fiber constituent protein (MYH7) gene in skeletal muscle cells to which the culture supernatant of lip-derived fibroblasts has been added. [Figure 6] A graph showing the expression levels of the muscle fiber constituent protein (MYH2) gene in skeletal muscle cells to which the culture supernatant of lip-derived fibroblasts with upregulated LINC00942 expression was added. [Figure 7] A graph showing the expression levels of the muscle fiber constituent protein (MYH7) gene in skeletal muscle cells to which the culture supernatant of lip-derived fibroblasts with upregulated LINC00942 expression was added. [Figure 8] Photographs of the lips of subjects who continuously used a lip cosmetic containing sage extract and crape myrtle extract. [Modes for carrying out the invention]

[0013] One aspect of the present invention is an expression promoter for LINC00942 containing one or more extracts from Lamiales plants selected from sage extract, perilla extract, lavender extract, rosemary extract, and verbena extract, as well as crape myrtle extract (hereinafter referred to as "expression promoter of the present invention"). It is also written as "advancement agent". The Lamiales plant extract is not particularly limited, but sage extract is more preferred. That is, the more preferred combination of extracts in the present invention is a combination of sage extract and crape myrtle extract. This invention arose when the inventors, seeking a new material to suppress skin aging, investigated various materials and discovered that rosmanol and its analogues, contained in extracts of Lamiales plants, have an effect of promoting the expression of LINC00942. The expression-promoting effect of LINC00942 in fibroblasts is achieved by combining it with Lagerstroemia indica extract. This is based on the finding that it tends to be further enhanced by [the following factor].

[0014] Rosmanol and its analogues are rosmanol and rosmanol-related compounds found in Lamiales plants such as sage, perilla, lavender, rosemary, and verbena. The Lamiales plant extracts used in the present invention typically contain one or more rosmanol and its analogues.

[0015] Examples of rosmanol and its analogues include compounds represented by the following formula (I). It is possible.

[0016] [ka]

[0017] In the formula, R 1 and R 2 Each of these is independently hydrogen, oxygen, hydroxyl, or an alkyloxy with 1 to 6 carbon atoms (preferably 1 to 3 carbon atoms, more preferably 1 to 2 carbon atoms). 1 and R 2 The compound is preferably hydroxyl or alkyloxy. As the alkyloxy having 1 to 6 carbon atoms, for example, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, tert-butoxy, n-pent oxy, n-hexyloxy and the like can be mentioned.

[0018] R 3 is carboxy, or alkoxycarbonyl having 1 to 6 carbon atoms (preferably 1 to 3 carbon atoms, more preferably 1 to 2 carbon atoms), or R 4 or R 5 together with -CO-O-. R 3 is preferably carboxy, or R 4 or R 5 together with -CO-O-. R 3 When R 4 or R 5 together with -CO-O-, R 3 not combined with R 4 or R 5 is preferably other than hydrogen. As the alkoxycarbonyl having 1 to 6 carbon atoms, for example, methoxycarbonyl, ethoxycarbonyl, n-propoxycarbonyl, isopropoxycarbonyl, n-butoxycarbonyl, isobutoxycarbonyl, sec-butoxycarbonyl, tert-butoxycarbonyl, n- pentoxycarbonyl, n-hexyloxycarbonyl and the like can be mentioned.

[0019] R 4 and R 5 are each independently hydrogen, oxygen, hydroxy, or alkyloxy having 1 to 6 carbon atoms (preferably 1 to 3 carbon atoms, more preferably 1 to 2 carbon atoms). Specific examples of the alkyloxy are the same as above. R 4 and R 5 are preferably hydrogen, hydroxy, or alkyloxy.

[0020] Each hydrogen atom bonded to the ring may be independently substituted with oxygen, a linear, branched, or cyclic alkyl group having 1 to 6 carbon atoms, a linear or branched alkenyl or alkynyl group having 2 to 6 carbon atoms, or an alkyloxy group having 1 to 6 carbon atoms. Examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, n-hexyl, cyclopropyl, and syl. Examples include clobutyl, cyclopentyl, and cyclohexyl. Alkenyl is a carbon atom having a carbon-carbon double bond at one or more locations on any of the alkyl groups. These are linear or branched alkenyls with 2 to 6 chain segments. Examples include vinyl, 1-propenyl, allyl, 1-butenyl, 2-butenyl, 3-butenyl, 1-pentenyl, 2-pentenyl, 3-pentenyl, 4-pentenyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 4-hexenyl, and 5-hexenyl. Alkynyl is a carbon atom having a carbon-carbon triple bond at one or more locations in the alkyl group. These are linear or branched alkynyl compounds with 2 to 6 chain segments. Examples include ethynyl, 1-propynyl, propargyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-pentynyl, 2-pentynyl, 3-pentynyl, 4-pentynyl, and 1-hexynyl. Specific examples of alkyloxys are the same as above.

[0021] Rosmanol and its analogues are, but are not limited to, carnosic acid, rosmanol, isorosmanol, 11,12-di-O-methylisorosmanol, 12-O-carnosic acid, rosmanol-9-ethyl ether, epirosmanol, and 7-methylepirosmanol, and are more preferably carnosic acid, rosmanol, or isorosmanol.

[0022] One or more extracts of Lamiales plants selected from sage extract, perilla extract, lavender extract, rosemary extract, and verbena extract used in the present invention can be obtained by extracting selected parts from the whole plant, fruit, leaves, stems, and roots of Lamiales plants with water and / or organic solvents. Furthermore, the crape myrtle extract used in the present invention can be obtained by extracting selected parts from the whole plant, fruit, leaves, stems, and roots of crape myrtle (Lagerstroemia speciosa) with water and / or an organic solvent. Organic solvents that can be used for extraction include aliphatic alcohols such as methanol and ethanol. Examples include aliphatic ketones such as chol and acetone, aliphatic ethers such as dioxane and ethyl ether, halogenated hydrocarbons such as methylene chloride and chloroform, fatty acid esters such as ethyl acetate, butyl acetate and isobutyl acetate, hydrocarbons such as hexane and benzene, and mixtures of two or more of these solvents.

[0023] In the present invention, the weight ratio of the total content of Lamiales plant extracts to the content of Lagerstroemia indica extract is preferably 20:1 to 100:1, and more preferably 45:1 to 100:1, when converted to dry weight.

[0024] Lamiales plant extracts promote the expression of LINC00942 in cells, as previously discovered. However (Patent Document 3), when crape myrtle extract is further combined, the expression-promoting effect tends to be further enhanced, as shown in the examples described later. Therefore, the combination of these extracts becomes the active ingredient of the expression-promoting agent LINC00942. Furthermore, fibroblasts are more preferably the cells in which LINC00942 expression is promoted, and lip-derived Fibroblasts are particularly preferred. Furthermore, "expression promotion" refers to the improvement of LINC00942 compared to when the expression promoting agent of the present invention is not applied. This refers to an increase in the expression level or rate. The degree of such increase is not particularly limited.

[0025] As disclosed in Patent Document 1, between the expression-promoting effect and the skin aging-inhibiting effect of LINC00942 A correlation exists between these two factors. Therefore, the expression-promoting agent of the present invention may exhibit a skin aging inhibitory effect. Another aspect of the present invention based on this is a composition for inhibiting skin aging, containing one or more extracts from Lamiales plants selected from sage extract, perilla extract, lavender extract, rosemary extract, and verbena extract, and crape myrtle extract. Here, "suppression of skin aging" refers to one or more of the following: prevention of decreased skin elasticity, prevention of wrinkles, prevention of sagging, prevention of blemish formation, prevention of dullness, and prevention of enlarged pores.

[0026] The present invention provides a composition for inhibiting skin aging, which includes the skin aging inhibitor of the present invention and may further contain ingredients used in cosmetics, quasi-drugs, foods, pharmaceuticals, etc., as long as they do not interfere with its effect, and can be used for inhibiting skin aging. The skin aging inhibitory composition of the present invention can be in the form of cosmetics, quasi-drugs, foods and beverages, pharmaceuticals, etc. The manner of intake (application) is not particularly limited, but in the case of cosmetics, quasi-drugs, and pharmaceuticals, it can be absorbed transdermally by applying the composition to the skin, and in the case of foods and beverages, it can be taken orally.

[0027] The total content of Lamiales plant extracts in the skin aging inhibitory composition of the present invention is not particularly limited, but is preferably 0.00001% by weight or more, more preferably 0.0001% by weight or more, and even more preferably 0.001% by weight or more, relative to the entire composition. Furthermore, there is no particular upper limit, but is preferably 30% by weight or less, more preferably 20% by weight or less, and even more preferably 10% by weight or less, relative to the entire composition. The total content of Lagerstroemia indicum extract in the skin aging inhibitory composition of the present invention is not particularly limited, but is preferably 0.00001% by weight or more, more preferably 0.0001% by weight or more, and even more preferably 0.001% by weight or more, relative to the entire composition. Furthermore, there is no particular upper limit, but is preferably 30% by weight or less, more preferably 20% by weight or less, and even more preferably 10% by weight or less, relative to the entire composition. In the skin aging inhibitory composition of the present invention, the weight ratio of the total content of Lamiales plant extracts to the content of Lagerstroemia speciosa extract is in accordance with the description of the expression promoter of the present invention described above.

[0028] The skin aging inhibitory composition of the present invention may contain any components as appropriate depending on the form. The skin aging inhibitory composition is prepared by conventional methods using the skin aging inhibitor of the present invention and It can be manufactured by formulating a mixture of any ingredients used in cosmetics, quasi-drugs, foods, pharmaceuticals, etc.

[0029] The present invention's composition for inhibiting skin aging is more preferably in the form of a cosmetic, and particularly preferably in the form of a lip cosmetic. This is because the expression of LINC00942 in lip fibroblasts is This is because it is expected that by increasing this effect, the elasticity of the lip skin will improve and wrinkles on the lips will be suppressed. Furthermore, it is expected that the anti-aging effect on the lips will result in youthful, fuller-looking lips.

[0030] Furthermore, since the expression promoter of the present invention acts on the skin through unidirectional or bidirectional interaction (intercellular communication) between adjacent cells, the expression promoter of the present invention or the skin aging inhibitory composition of the present invention can be suitably used as a lip cosmetic. As shown in the reference example below, when skeletal muscle cells were cultured with the supernatant from cultured lip-derived fibroblasts, muscle fiber constituent proteins (myosin heavy chain proteins; MYH2, MYH7) increased. This suggests that lip-derived fibroblasts release some substance involved in the activity of skeletal muscle cells. In other words, it is thought that intercellular communication exists between lip-derived fibroblasts and skeletal muscle cells. Furthermore, as shown in the examples described later, lip-derived fibroblasts with enhanced LINC00942 expression When the culture supernatant was added to skeletal muscle cells and cultured, the gene expression levels of MYH2 and MYH7 increased. Therefore, when LINC00942 expression is enhanced in lip-derived fibroblasts, skeletal muscle cells... It is hypothesized that this can increase the release of some substance involved in the activity of the muscle, thereby increasing the amount of muscle fiber constituent proteins in adjacent skeletal muscle cells. It is known that muscle fiber constituent proteins decrease with age (T. Gomi, et al., Intr. J. Cosmetic Sci., 2020, 1-10), but LINC00942 restores muscle fiber constituent proteins. These can be considered anti-aging genes from the perspective of promoting aging.

[0031] Therefore, Lamiales plant extracts that promote LINC00942 expression in lip-derived fibroblasts The combination with Lagerstroemia indica extract is said to have an effect of increasing the muscle fiber constituent proteins of the lips. Therefore, by incorporating this combination into lip cosmetics, it is possible to increase the muscle fiber constituent proteins of the lips, which is expected to result in three-dimensional, thick, and voluminous lips, thus creating youthful or attractive lips. Therefore, the lip cosmetic of the present invention may be used to increase the muscle fiber constituent proteins of the lips. Here, "increase" of muscle fiber constituent proteins includes an increase in the expression level or expression rate of muscle fiber constituent proteins or their genes compared to when the expression promoter of the present invention, the skin aging inhibitory composition of the present invention, or the cosmetic composition of the present invention is not applied, and suppression of a decrease in said expression level or expression rate.

[0032] Traditionally, firm, plump lips have been seen as giving a youthful appearance, and methods to achieve this have been in high demand. For example, there are methods to create a plump appearance by applying cosmetics such as lip gloss. In addition, hyaluronic acid is known as an ingredient that gives firmness and volume to the skin, and there are cosmetics that contain ingredients that increase the amount of hyaluronic acid in the dermis, as well as cosmetic medical procedures that directly give firmness and volume to the lips by injecting hyaluronic acid. In this context, cosmetics that increase the muscle fiber constituent proteins of the lips are considered a novel and groundbreaking method that meets consumer demand, as they can provide firmness and volume to the lips as an alternative to hyaluronic acid, creating a youthful appearance.

[0033] The embodiments of the lip cosmetic of the present invention are not particularly limited to lip cream, lipstick, lip gloss, lip balm, etc.

[0034] This section describes the components that can be included when the present invention's skin aging inhibitory composition is in the form of a cosmetic. In addition to the active ingredients of the present invention, namely the Lamiales plant extract and Lagerstroemia indica extract, the skin aging inhibitory composition of the present invention may further contain active ingredients having the same or different functions. Active ingredients include skin whitening ingredients, wrinkle-improving ingredients, anti-inflammatory ingredients, and extracts derived from plants and animals.

[0035] There are no particular limitations on the whitening ingredients, as long as they are commonly used in cosmetics. For example, 4-n-butylresorcinol, ascorbic acid glucoside, 3-O-ethyl ascorbic acid Examples include benzoyl phosphate, tranexamic acid, arbutin, 1-triphenylmethylpiperidine, 1-triphenylmethylpyrrolidine, 2-(triphenylmethyloxy)ethanol, 2-(triphenylmethylamino)ethanol, 2-(triphenylmethyloxy)ethylamine, triphenylmethylamine, triphenylmethanol, triphenylmethane and aminodiphenylmethane, N-(p-toluyl)cysteic acid, N-(p-methoxybenzoyl)cysteic acid, etc. Furthermore, other whitening ingredients include N-benzoyl-serine, N-(p-methylbenzoyl)serine, N-(p-ethylbenzoyl)serine, N-(p-methoxybenzoyl)serine, N-(p-fluorobenzoyl)serine, N-(p-trifluoromethylbenzoyl)serine, N-(2-naphthoyl)serine, N-(4-phenylbenzoyl)serine, N-(p-methylbenzoyl)serine methyl ester, N-(p-methylbenzoyl)serine ethyl ester, N-(2-naphthoyl)serine methyl ester, N-benzoyl-O-methylserine, N-(p-methylbenzoyl Examples include N-(p-methylbenzoyl)-O-acetylserine, N-(2-naphthoyl)-O-methylserine, niacinamide, and D-pantothenyl alcohol.

[0036] Some of these whitening ingredients are already commercially available, while others can be obtained through synthesis. For example, 3-O-ethyl ascorbic acid is a known substance described in Japanese Patent Publication No. 8-134055. It can be synthesized by law. There are also commercially available products ("VC Ethyl" manufactured by Nippon Seika), so it is possible to obtain and use these. 1-Triphenylmethylpiperidine, 1-Triphenylmethylpyrrolidine, 2-(triphenylmethyloxy)ethanol, 2-(triphenylmethylamino)ethanol, 2-(triphenylmethyloxy)ethylamine, triphenylmethylamine, triphenylmethanol, triphenylmethane, and aminodiphenylmethane are mentioned in Patent Document WO / 2010 / 074052, and N-(o-Toluroyl)cysteic acid, N-(m-Toluroyl)cysteic acid, N-(p-Toluroyl)cysteic acid, N-(p-methoxybenzoyl)cysteic acid, N-(4-phenylbenzoyl)cysteic acid, N-(p-Toluroyl) Homocysteic acid (oil) is listed in WO / 2011 / 058730 pamphlet as N-benzoyl-serine, N-(p-methylbenzoyl)serine, N-(p-ethylbenzoyl)serine, N-(p-methoxybenzoyl)serine, N-(p-fluorobenzoyl)serine, N-(p-trifluoromethylbenzoyl)serine, N-(2-naphthoyl)serine, N-(4-phenylbenzoyl)serine, N-(p-methylbenzoyl)serine methyl ester, N-(p-methylbenzoyl)serine ethyl ester, N-(2-naphthoyl)serine methyl ester, N-benzoyl-O-methylserine, N-(p-methylbenzoyl) Zoyl)-O-methylserine, N-(p-methylbenzoyl)-O-acetylserine, N-(2-naphthoyl)-O-methylserine, etc., have their synthesis methods described in WO / 2011 / 074643. Since it is publicly available, it can be synthesized in accordance with the disclosure.

[0037] There are no particular limitations on the wrinkle-improving ingredients, as long as they are commonly used in cosmetics. For example, vitamin A or its derivatives, isopropyl trifluoride oxopropylaminophosphate Sodium nitrate, nitrate, cyclohexylglycerin, retinol, retinal, retinoic acid, tretinoin, isotretinoin, retinoic acid tocopherol, palmitate Examples include tinol, retinyl acetate, benzyl ursolate, ursolic acid phosphate, benzyl betulinate, benzyl acid phosphate, N-(p-toluyl)cysteic acid, and N-(p-methoxybenzoyl)cysteic acid.

[0038] There are no particular limitations on the use of plant and animal-derived extracts, as long as they are commonly used in pharmaceuticals, cosmetics, foods, etc. For example, Akebia extract, Thujopsis dolabrata extract, Asparagus extract, Avocado extract, Hydrangea macrophylla extract, Almond extract, Arnica extract, Aloe extract, Aronia extract, Apricot extract, Rosa rugosa extract, Ginkgo biloba extract, Indian kinosum extract, Fennel extract, Aralia cordata extract, Rosa multiflora extract, Eleutherococcus senticosus extract, Scutellaria baicalensis extract, Phellodendron amurense extract, Coptis japonica extract, Panax ginseng extract, Hypericum perforatum extract, Lamium album extract, Orange extract, Citrus aurantium umbellata extract, Hydrolyzed conchiolin liquid Hydrolyzed silk, kudzu extract, chamomile extract, carrot extract, Artemisia capillaris extract, licorice extract, kiwi extract, cucumber extract, guava extract, Sophora flavescens extract, gardenia extract, Sasa veitchii extract, Sophora flavescens extract, walnut extract, grapefruit extract, black rice extract, clove extract, chlorella extract, mulberry extract, Alpinia speciosa extract, Gentiana extract, Geranium thunbergii extract, black tea extract, burdock extract, rice extract, rice ferment extract, rice bran ferment extract, rice germ oil, Lingonberry extract, Salvia extract, Soapwort extract, Bamboo extract, Hawthorn extract, Sunflower extract, Japanese pepper extract, Shiitake mushroom extract, Rehmannia extract, Lithospermum extract, Linden extract, Spiraea extract, Peony extract, Ginger extract, Calamus root extract, Birch bark extract, Horsetail extract, Star fruit extract, Stevia extract, Stevia ferment, Hedera helix extract, Hawthorn extract, Elderberry extract, Yarrow extract, Peppermint extract, Malva extract Succulent extract, Cnidium officinale extract, Swertia japonica extract, Morus alba bark extract, Rhubarb extract, Soybean extract, Jujube extract, Thyme extract, Dandelion extract, Tea extract, Clove extract, Citrus unshiu peel extract, Sweet tea extract, Capsicum extract, Angelica acutiloba extract, Calendula officinalis extract, Prunus persica extract, Prunus serrulata extract, Prunus serrulata extract, Houttuynia cordata extract, Tomato extract, Natto extract, Carrot extract, Garlic extract, Rosehip extract, Hibiscus extract, Ophiopogon japonicus extract, Lotus extract, Parsley extract, Birch extract,Preferred extracts include Job's tears seed extract, witch hazel extract, Isodon japonicus extract, cypress extract, loquat extract, coltsfoot extract, butterbur extract, Poria cocos extract, butcher's broom extract, grape extract, grape seed extract, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, marjoram extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, lemon balm extract, seaweed extract, peach extract, peach leaf extract, cornflower extract, eucalyptus extract, saxifrage extract, yuzu extract, lily extract, coix seed extract, mugwort extract, green tea extract, lime extract, apple extract, rooibos tea extract, reishi mushroom extract, lettuce extract, lemon extract, forsythia extract, astragalus extract, rose extract, Roman chamomile extract, royal jelly extract, and Sanguisorba officinalis extract.

[0039] Examples of anti-inflammatory components include clarinon, glabridin, glycyrrhizic acid, glycyrrhetinic acid, pantothenyl alcohol, tranexamic acid, and niacinamide, with glycyrrhizic acid and its salts being preferred, alkyl glycyrrhetinate and its salts, and glycyrrhetinic acid and its salts.

[0040] Other ingredients that may be included in cosmetics are listed below as examples. Examples of oily components include polar oils and volatile hydrocarbon oils. As polar oils, synthetic ester oils include isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, decyl oleate, and dimethyloctanoic acid. Hexyldecyl, cetyl lactate, myristyl lactate, lanolin acetate, isocetyl stearate, isocetyl isostearate, cholesteryl 12-hydroxystearylate, ethylene glycol di-2-ethylhexylate, dipentaerythritol fatty acid ester, N-alkyl glycol monoisostearate, neopentyl glycol dicaprate, diisostearyl malate, glycerin di-2-heptylundecanoate, trimethylolpropane tri-2-ethylhexylate, trimethylolpropane triisostearate, pentaneerythritol tetra-2-ethylhexylate, glycerin tri-2-ethylhexylate, triisos Trimethylolpropane thearate can be cited as an example. Furthermore, it contains cetyl 2-ethylhexanoate, 2-ethylhexyl palmitate, glyceryl trimyristate, glyceride tri-2-heptyl undecanoate, and methyl castor oil fatty acid. Esters, oleic acid oil, cetostearyl alcohol, acetoglyceride, 2-heptyl undecyl palmitate, diisobutyl adipate, N-lauroyl-L-glutamine Acid-2-octyldodecyl ester, di-2-heptylundecyl adipate, ethyl laurate, di-2-ethylhexyl sebatate, 2-hexyldecyl myristate, palmitate 2 Other examples include hexyldecyl, 2-hexyldecyl adipic acid, diisopropyl sebatate, 2-ethylhexyl succinate, ethyl acetate, butyl acetate, amyl acetate, triethyl citrate, and octyl methoxycinnamate. Other examples of natural oils include avocado oil, camellia oil, turtle oil, macadamia nut oil, corn oil, mink oil, olive oil, rapeseed oil, egg yolk oil, sesame oil, peach kernel oil, wheat germ oil, sasanqua oil, castor oil, linseed oil, safflower oil, cottonseed oil, elm oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, cinnamon oil, Japanese tung oil, jojoba oil, wheat germ oil, triglycerin, glyceryl trioctanoate, and glyceryl triisopalmitate.

[0041] Examples of volatile hydrocarbon oils include isododecane and isohexadecane.

[0042] Examples of surfactants include fatty acid soaps (sodium laurate, sodium palmitate, etc.), anionic surfactants such as potassium lauryl sulfate and alkyl sulfate triethanolamine ether, cationic surfactants such as stearyltrimethylammonium chloride, benzalkonium chloride, and laurylamine oxide, betaine-based surfactants (alkyl betaine, amide betaine, sulfobetaine, etc.), imidazoline-based amphoteric surfactants (2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy disodium salt, etc.), and Amphoteric surfactants such as silmethyl taurine, sorbitan fatty acid esters (sorbitan monostearate, sorbitan sesquioleate, etc.), glycerin fatty acids (glyceryl monostearate, etc.), propylene glycol fatty acid esters (propylene glycol monostearate, etc.), hydrogenated castor oil derivatives, glycerin alkyl ethers, POE sorbita POE fatty acid esters (POE sorbitan monooleate, polyoxyethylene monostearate) (e.g., Rensorbitan), POE sorbitan fatty acid esters (POE-sorbitan monolaurate) (etc.), POE glycerin fatty acid esters (POE-glycerin monoisostearate, etc.), POE fatty acid esters (polyethylene glycol monooleate, POE distearate, etc.), POE alkyl ethers (POE 2-octyldodecyl ether, etc.), POE alkylphenyl ethers (POE nonylphenyl ether, etc.), Pluronic® type, POE·POPal Kill ethers (POE·POP2-decyltetradecyl ether, etc.), tetronics, POE hyphens Examples include castor oil and hydrogenated castor oil derivatives (such as POE castor oil and POE hydrogenated castor oil), sucrose fatty acid esters, and nonionic surfactants such as alkyl glucosides.

[0043] Polyhydric alcohols include polyethylene glycol, glycerin, 1,3-butylene glycol, erythritol, sorbitol, xylitol, maltitol, propylene glycol, dipropylene glycol, diglycerin, isoprene glycol, 1,2-pentanediol, 2,4-hexylene glycol, 1,2-hexanediol, 1,2-octanediol, etc. It can be listed.

[0044] Examples of thickening agents include guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, curdlan, methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, methylhydroxypropylcellulose, chondroitin sulfate, dermatan sulfate, glycogen, heparan sulfate, hyaluronic acid, sodium hyaluronate, tragacanth gum, keratan sulfate, chondroitin, mucoitin sulfate, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, kerato sulfate, locust bean gum, succinoglycan, carotenoid acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate, polyethylene glycol, and bentonite.

[0045] Powders may include: powders such as mica, talc, kaolin, synthetic mica, calcium carbonate, magnesium carbonate, anhydrous silicic acid (silica), aluminum oxide, and barium sulfate, which may have surface treatments; inorganic pigments such as red iron oxide, yellow iron oxide, black iron oxide, cobalt oxide, ultramarine, Prussian blue, titanium dioxide, and zinc oxide, which may have surface treatments; pearlescent agents such as titanium mica, fish scale foil, and bismuth oxychloride, which may have surface treatments; and Red No. 202, Red No. 228, Red No. 226, Yellow No. 4, Blue No. 404, Yellow No. 5, Red No. 505, which may be lake-formed. Examples include organic dyes such as Color No. 230, Red No. 223, Orange No. 201, Red No. 213, Yellow No. 204, Yellow No. 203, Blue No. 1, Green No. 201, Violet No. 201, and Red No. 204, as well as organic powders such as polyethylene powder, polymethyl methacrylate, nylon powder, and organopolysiloxane elastomers.

[0046] Examples of UV absorbers include para-aminobenzoic acid-based UV absorbers, anthranilic acid-based UV absorbers, salicylic acid-based UV absorbers, cinnamic acid-based UV absorbers, benzophenone-based UV absorbers, sugar-based UV absorbers, and 2-(2'-hydroxy-5'-t-octylphenyl)benzotriaz Examples include UV absorbers such as 4-methoxy-4'-t-butyldibenzoylmethane.

[0047] Cosmetics may also contain other ingredients such as water, ethanol, fragrance, preservatives, pH adjusters, and colorants.

[0048] Furthermore, when applied as a cosmetic, the dosage form can be any of the commonly known forms, such as lotion, emulsion, essence, cream, or powder-containing forms, in addition to the forms mentioned above for lip cosmetics. [Examples]

[0049] The present invention will be specifically described below with reference to examples and reference examples, but these are illustrative examples of the present invention and the scope of the present invention is not limited thereto.

[0050] <Material> The plant extracts used in the evaluation were commercially available plant extracts. Specifically, the following were used: Perilla extract (Amino Up Co.), Lavender extract (Maruzen Pharmaceutical Co.), Sage extract (Ichimaru Falcos Co.), Rosemary extract (Koei Kogyo Co.), Verbena extract (Seti Co.), Morus alba bark extract (Maruzen Pharmaceutical Co.), Clove extract (Ichimaru Falcos Co.), Carrot extract (Maruzen Pharmaceutical Co.), Poria cocos extract (Ichimaru Falcos Co.), Lime extract (Maruzen Pharmaceutical Co.), Lagerstroemia indica extract (Mikimoto Pharmaceutical Co.), Eucalyptus extract (Koei Kogyo), Catechu extract (Koei Kogyo), Linden extract (Koei Kogyo), Kouki extract (Maruzen Pharmaceutical Co.), and Sen'nin oak seed extract (Koei Kogyo). The compounds used for evaluation were commercially available compounds, namely rosmanol. (PhytoLab), isosmanol (Sigma-Aldrich), and carnosic acid (Tokyo Chemical Industries, Ltd.) were used.

[0051] <Reference Example 1> Confirmation test of LINC00942 expression promotion effect by Lamiales plant extracts Dermal fibroblasts derived from a 68-year-old Caucasian were used as aged cells. Fibroblasts, subcultured in 10% FBS-containing DMEM medium, were seeded into 96-well plates at a density of 7000 cells / well and cultured for 24 hours at 37°C under 5% CO2 conditions. Subsequently, the medium was replaced with the respective concentrations (v / v%) of each plant extract shown in Table 1, and cultured for another 24 hours. As a solvent control, each plant extract was cultured in the same manner in a medium containing the same concentration (v / v%) as the evaluated extract (3 wells per group). After culturing, cells were harvested, and the expression level of LINC00942 was analyzed by quantitative real-time PCR using the FastLane Cell SYBR Green Kit (Qiagen, 216213) and LINC00942-specific primers (Qiagen, Hs_LINC00942_1_SG). The relative value was calculated with the expression level in the solvent control set to 1. The results were determined. Based on the results, Dunnett's test was performed on the solvent control group and compared with the solvent control group. We evaluated whether the value was significantly large.

[0052] The results are shown in Table 1. In Table 1, the plant names are as follows: Perilla extract is abbreviated as Perilla, Lavender extract as Lavender, Sage extract as Sage, Rosemary extract as Rosemary, Verbena extract as Verbena, Mulberry bark extract as Mulberry, Clove extract as Clove, Carrot extract as Carrot, Poria extract as Poria, Lime extract as Lime, Ethanol as EtOH, and 1,3-Butylene glycol as BG. * indicates p<0.05, ** indicates p<0.01, and NS indicates no significant difference. Perilla extract, lavender extract, sage extract, rosemary extract, and verbena extract, all extracts from plants in the Lamiales order, were found to have excellent LINC00942 expression-promoting effects.

[0053] [Table 1]

[0054] <Reference Example 2> Confirmation test of LINC00942 expression promotion effect by rosmanol and its analogues The expression-promoting effects of various compounds found in Lamiales plants were evaluated for LINC00942. Dermal fibroblasts derived from a 68-year-old Caucasian were used as aged cells. Fibroblasts, subcultured in 10% FBS-containing DMEM medium, were seeded into 96-well plates at a density of 7000 cells / well and cultured for 24 hours at 37°C under 5% CO2 conditions. Subsequently, they were soluble in DMSO and evaluated. The culture medium was replaced with one containing the compound (Table 2) at a final concentration of 0.0005% (w / v), and the cells were incubated for a further 24 hours. The cells were similarly cultured in a medium containing 0.1% (v / v%) DMSO as a solvent control. At the end of the culture period, the expression level of LINC00942 was analyzed by quantitative real-time PCR using the FastLane Cell SYBR Green Kit (Qiagen, 216213) and LINC00942-specific primers (Qiagen, Hs_LINC00942_1_SG). The relative value was calculated with the expression level of the solvent control set to 1. This was performed with 3 wells per group, n=3. Based on the results, Dunnett's test was performed against the solvent control group to evaluate whether the values ​​were significantly higher than those of the solvent control group.

[0055] The results are shown in Table 2. DMSO in Table 2 refers to dimethyl sulfoxide. * indicates p<0.05, and ** indicates p<0.01. Rosmanol and its analogues, rosmanol, isorosmanol, and carnosic acid, found in plants of the Lamiales order, were found to have excellent LINC00942 expression-promoting effects.

[0056] [Table 2]

[0057] <Example 1> LINC00942 expression promotion effect by combining Lamiales plant extract with other plant extracts Confirmation test 1000 μL of normal human lip-derived fibroblasts NF125 (Chinese, Female, Cell Research Corporation), suspended in 10% FBS-containing D-MEM medium, were seeded into 24-well plates (3.0 × 10⁶). 4 (cells / mL). After culturing at 37°C in a 5% CO2 environment for 24 hours, the medium is removed, and 450 μL of sage extract-containing medium (final extract concentration 0.5 v / v%) and 50 μL of another extract-containing medium (final extract concentration 0.025 v / v%) are added. ) was added to each well (4 wells for each group). Other extracts included crape myrtle. Extracts of eucalyptus, catechu extract, linden extract, kouki extract, or sen'nin okina seed extract were used. After culturing for a further 24 hours at 37°C in a 5% CO2 environment, cells were harvested and processed using Fast SYBR Green Master Mix (Applied: 4385617), RNeasy Mini Kit-8 (250) (Qiagen: 74106), and Superscript VILO cDNA Synthesis kit 250T (Invitrogen). Using LINC00942 expression levels (11754-250), Hs_LINC00942_2_SG QuantiTect Primer Assay (QIAGEN:QT02305933), and Hs_ACTB_2_SG QuantiTect Primer Assay (QIAGEN:QT01680476), quantitative real-time PCR was used to analyze LINC00942 expression levels, with the expression level in the solvent control set to 1. The relative values ​​were calculated. Based on the results, Dunnett's test was performed against the solvent control group.

[0058] The results are shown in Figure 1. The expression of the LINC00942 gene was higher in lip-derived fibroblasts treated with both sage extract and crape myrtle extract compared to those treated with sage extract alone. An increasing trend was observed.

[0059] <Reference Example 3> Effects of lip-derived fibroblast culture supernatant on muscle fiber constituent proteins in skeletal muscle cells 1000 μL of normal human lip-derived fibroblasts NF125 (Chinese, Female, Cell Research Corporation), suspended in 10% FBS-containing D-MEM medium, were seeded into 24-well plates (3.0 × 10⁶). 4 Cells / mL). After culturing at 37°C in a 5% CO2 environment for 24 hours, the supernatant was collected at a rate of 300 μL / well. 500 μL of normal human skeletal muscle myoblasts (HSMM) (Caucasian, Female, CC-2580, Lonza) suspended in growth medium (SkGM-2 BulletKit (Lonza: CC-3245)) were seeded into a 24-well plate (9.0 ×10 4 (cells / mL). After culturing at 37°C in a 5% CO2 environment for 24 hours, the medium was removed and washed with 1000 μL / well of PBS. Then, 250 μL / well of differentiation medium (SkMC Differentiation medium (TaKaRa:D12045)) and 250 μL / well of the previously collected lip-derived fibroblast culture supernatant diluted in D-MEM medium to 100%, 50%, or 25 (v / v)% were added. A solvent control was also used. Differentiation medium 250 μL / well and D-MEM medium 250 μL / well were added. The mixture was then incubated at 37°C in a 5% CO2 environment for 4 minutes. After culturing for 8 hours, skeletal muscle cells were harvested, and the expression levels of muscle fiber constituent protein (MYH2, MYH7) genes were measured by quantitative real-time PCR using Superscript VILO cDNA Synthesis kit 250T (Invitrogen: 11754-250), RNeasy Mini Kit-8 (250) (QIAGEN: 74106), Fast SYBR Green Master Mix (Applied: 4385617), Hs_MYH2_1_SG QuantiTect Primer Assay (QIAGEN: QT00082495), Hs_MYH7_1_SG QuantiTect Primer Assay (QIAGEN: QT01680476), and Hs_ACTB_2_SG QuantiTect Primer Assay (QIAGEN: QT01680476). The results were analyzed, and relative values ​​were determined when the expression level in the solvent control was set to 1. Based on the results, the solvent-to-solution ratio was calculated. A Dunnett test was performed on the illuminated group. Furthermore, skeletal muscle cells cultured in a final concentration of 50(v / v)% lip-derived fibroblast culture supernatant as described above were cultured at 37°C in a 5% CO2 environment for 72 hours. These cells were then immunofluorescently stained with anti-MYH2 antibody or anti-MYH7 antibody and observed under a fluorescence microscope.

[0060] Figures 2 and 3 show fluorescent staining images of skeletal muscle cells. It can be seen that skeletal muscle cells cultured with supernatant from lip-derived fibroblasts showed increased levels of both MYH2 and MYH7 compared to the control. Figures 4 and 5 show the relative expression levels of the MYH2 and MYH7 genes in skeletal muscle cells. It can be seen that the expression levels of both the MYH2 and MYH7 genes are elevated in skeletal muscle cells cultured with supernatant from lip-derived fibroblasts compared to the control.

[0061] <Example 2> Skeletal muscle cells from the supernatant of lip-derived fibroblast cultures with enhanced LINC00942 expression Effects on fiber constituent proteins 1000 μL of normal human lip-derived fibroblasts NF125 (Chinese, Female, Cell Research Corporation), suspended in 10% FBS-containing D-MEM medium, were seeded into 24-well plates (3.0 × 10⁶). 4 Cells / mL). The cells were incubated at 37°C in a 5% CO2 environment for 24 hours. Plasmids were transfected into cultured fibroblasts. Specifically, Lipofectamine 3000 Reagent (Thermofisher Scientific) was diluted in Opti-MEM medium, vortexed for 2-3 seconds, and gently centrifuged. Equal volumes of each were then mixed with the plasmid (control plasmid: pcDNA-DEST47 (Thermofisher Scientific), or LINC00942 expression plasmid: pcDNA-DEST47-3E) and P3000, diluted in Opti-MEM medium, pipettered, and thoroughly mixed. These mixtures were then incubated for 5 minutes. The plasmid solution was incubated at room temperature. 50 μL of the incubated plasmid solution and 500 μL of D-MEM medium were used. μL was added to fibroblasts and cultured for 72 hours at 37°C in a 5% CO2 environment. After culturing, the culture medium was used. After removing the fibroblasts and washing them with 1000 μL / well of PBS, 500 μL / well of D-MEM medium containing 10% FBS was added, and the cells were cultured for 24 hours at 37°C under 5% CO2 conditions. 300 μL / well of the supernatant was collected after culturing. 500 μL of normal human skeletal muscle myoblasts (HSMM) (Caucasian, Female, CC-2580, Lonza) suspended in growth medium (SkGM-2 BulletKit (Lonza: CC-3245)) were seeded into a 24-well plate (9.0 × 10⁶). 4 (cells / mL). After culturing at 37°C in a 5% CO2 environment for 24 hours, remove the culture medium and add PBS 1000. The cells were washed at a rate of μL / well. Then, 250 μL / well of differentiation medium (SkMC Differentiation medium (TaKaRa:D12045)) and 250 μL / well of the previously collected lip-derived fibroblast culture supernatant diluted to 25 (v / v)% in D-MEM medium were added. The cells were incubated at 37°C in a 5% CO2 environment for 48 hours. Afterward, skeletal muscle cells were collected and, as in Reference Example 3, were analyzed using quantitative real-time PCR. The expression levels of fibroblast constituent protein (MYH2, MYH7) genes were analyzed, and their relative values ​​were determined, with the expression level when fibroblast culture supernatant containing a control plasmid was added set to 1. Meta.

[0062] Figures 6 and 7 show the relative expression levels of the MYH2 and MYH7 genes in skeletal muscle cells. In cultures of lip-derived fibroblasts in which LINC00942 expression was enhanced by introducing an expression plasmid, In skeletal muscle cells cultured with purified water, the expression levels of the MYH2 and MYH7 genes were elevated compared to the control group. As shown in Reference Example 3, lip-derived fibroblasts can release substances involved in the activity of skeletal muscle cells and increase muscle fiber constituent proteins in skeletal muscle cells, and this effect is further enhanced when LINC00942 expression is increased in lip-derived fibroblasts, as shown in Example 2. This can be understood from that.

[0063] <Example 3> Investigation of the effects on the lips of continuous use of lip cosmetics containing sage extract and crape myrtle extract. All of the formulation ingredients shown in Table 3 were mixed and stirred until uniformly dispersed to prepare a liquid lip cosmetic containing sage extract and crape myrtle extract.

[0064] [Table 3]

[0065] Twenty-five healthy Japanese adult women (ages 30-54, average age 43.32) voluntarily participated in a continuous use test of the aforementioned cosmetic product. The subjects were those who had concerns about their lips, such as a lack of volume. Each subject applied an appropriate amount of sunscreen (SPF50, PA+++) to their entire face every morning, and applied an appropriate amount of the aforementioned lip cosmetic product to their lips every morning, noon, and evening. The subjects' lips were evaluated at 0, 4, and 12 weeks after the start of continuous use. Specifically, after removing makeup with cleansing and face wash, the skin was acclimatized for 10 minutes in a constant temperature and humidity chamber (around 23°C, humidity 50±5%). Then, using a digital SLR camera, with a color chart for image correction attached to the brow, the face was photographed from the front, at 45 degrees to the left and right, and at 90 degrees to the left and right with the eyes open. Figure 8 shows photographs of two subjects (Subjects A and B) whose lip volume and vertical wrinkles significantly improved at 4 weeks and 12 weeks, respectively.

Claims

1. Expression of LINC00942 containing one or more extracts of Lamiales plants selected from sage extract, lavender extract, and verbena extract, as well as crape myrtle extract. Accelerator.

2. An expression promoter according to claim 1, which promotes the expression of LINC00942 in lip-derived fibroblasts. 。

3. The expression promoter according to claim 1 or 2, wherein the dry weight ratio of the content of Lamiales plant extract to Lagerstroemia indica extract is 20:1 to 100:

1.

4. A composition for inhibiting skin aging, containing one or more extracts from Lamiales plants selected from sage extract, lavender extract, and verbena extract, and crape myrtle extract.

5. A lip cosmetic containing one or more extracts from Lamiales plants selected from sage extract, lavender extract, and verbena extract, and crape myrtle extract.

6. The cosmetic composition according to claim 5, used to increase the muscle fiber constituent proteins of the lips.

Images