Method for improving the γ-aminobutyric acid synthesis ability of Lactobacillus brevis and its use

Abstract

The glnR gene is knocked out by homologous recombination to obtain a Lactobacillus brevis glnR knockout strain, which improves the ability of Lactobacillus brevis to synthesize gamma-aminobutyric acid and tolerate acidic environments, and its use is provided.The Lactobacillus brevis glnR knockout strain is fermented using acid control and fed-batch fermentation, resulting in a cumulative GABA concentration of 301.5 g / L with a production efficiency of up to 5.76 g / L / h, making it suitable for large-scale GABA production, reducing GABA production costs, and being used in the fermentation of acidic foods, etc.

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