改良された核酸検出方法
JP7891794B2Inactive Publication Date: 2026-07-17TOYOBO CO LTD
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- TOYOBO CO LTD
- Filing Date
- 2019-01-23
- Publication Date
- 2026-07-17
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Benefits of technology
【0013】 本発明によって、核酸増幅反応に先立って核酸を分離精製したり、試料を加熱処理したりするための時間·コストを削減することができる。さらに、核酸を分離精製や試料の加熱処理を事前に実施する際に生じる可能性のあるリスク、すなわち試料のロスやキャリーオーバーによる他のサンプルへの汚染の危険性を低減することができる。特に糞便を試料とする多数の検体を処理するような検査において、その効果は顕著となる。
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Abstract
To reduce the effect of substances contained in a biological sample that inhibit, over time, the nucleic acid amplification reaction, in particular, to suppress the degradation of RT-PCR efficiency even if the sample is not subjected to nucleic acid isolation and purification treatment or the like when feces are sampled.SOLUTION: The present invention relates to a method of examining the presence or absence of a target RNA in a sample, the method including the following steps: (1) adding a One-enzyme system 1-step RT-PCR reaction solution comprising a divalent cation and a thermostable DNA polymerase that are adjusted for a final concentration of 2.5 mM or more in the reaction solution to a sample that has not been subjected to nucleic acid separation / purification treatment or heat treatment; and (2) examining the presence or absence of the target RNA in the sample by a 1-step RT-PCR reaction after sealing the reaction container.SELECTED DRAWING: None
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