ポリヌクレオチド断片の分離

Abstract

Methods are provided that include extending a polynucleotide onto a substrate comprising a plurality of equally spaced cleavage regions comprising a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments within the plurality of polynucleotide fragments. Methods are also provided that include extending a polynucleotide onto a substrate comprising a plurality of equally spaced cleavage regions comprising a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments within the plurality of polynucleotide fragments.

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