Methods for saccharification of cellulose

Abstract

To provide a method for saccharifying cellulose, which prevents an enzyme reaction from being inhibited by impurities in the raw material, which are oil components such as lignin or pitch, and also allows the inside of a saccharification tank to be easily kept clean.SOLUTION: A method for saccharifying cellulose includes the steps for introducing a cellulose-containing raw material and a reaction solution into a saccharification tank, introducing an enzyme into the reaction solution in the saccharification tank, disposing an adsorption sheet on the liquid surface of the reaction solution, and decomposing the cellulose with the enzyme by stirring the reaction solution to produce saccharides. The adsorption sheet adsorbs an oil component released from the raw material to the liquid surface of the reaction solution during the production step of the saccharides.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a method for saccharifying cellulose.

Background Art

[0002] Obtaining glucose by saccharifying cellulose has attracted attention from the viewpoints of so-called carbon minus, effective utilization of biomass, application to bioethanol, etc. For example, Patent Document 1 discloses a method for producing sugar by saccharification using paper as a raw material. In the technique described in Patent Document 1, a method for obtaining sugar through pretreatment and enzymatic saccharification using waste paper as a cellulose raw material is disclosed.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] When saccharifying cellulose, there is a method in which a saccharification reaction solution containing an enzyme and a raw material are charged into a saccharification tank, and stirring is performed to advance the enzyme reaction to saccharify cellulose. However, when using paper as a cellulose raw material, the paper contains not only cellulose but also pitch (adhesive substances), and in the case of used waste paper, oily components of ink, etc., components insoluble in the saccharification reaction solution. These components are not saccharified, so they not only adhere to cellulose and inhibit the enzyme reaction, thereby inhibiting saccharification, but may also remain in the reaction solution and remain as unnecessary components such as sludge on the inner wall of the saccharification tank.

[0005] Therefore, there is a need for a method for saccharifying cellulose in which the inhibition of the enzyme reaction due to impurities such as lignin and pitch in the raw material is unlikely to occur, and it is easy to keep the inside of the saccharification tank clean. [Means for solving the problem]

[0006] One aspect of the cellulose saccharification method according to the present invention is: The process involves introducing a raw material containing cellulose and a reaction solution into a saccharification tank. The steps include introducing an enzyme into the reaction solution in the saccharification tank, The steps include: placing an adsorbent sheet on the surface of the reaction solution; The process involves stirring the reaction solution and generating sugar by decomposing the cellulose with the enzyme, Includes, The adsorption sheet adsorbs oily components released from the raw materials to the surface of the reaction solution during the process of producing the sugar, in a method for saccharifying cellulose. [Brief explanation of the drawing]

[0007] [Figure 1] A schematic diagram showing an overview of the saccharification treatment according to the example. [Figure 2] A schematic diagram of the adsorption sheet according to the example, viewed in plan view. [Figure 3] A schematic diagram of the suction sheet according to the modified embodiment, viewed in plan view. [Modes for carrying out the invention]

[0008] Embodiments of the present invention are described below. The embodiments described below illustrate examples of the present invention. The present invention is not limited in any way to the embodiments described below and includes various modifications that are implemented without changing the gist of the present invention. Not all of the configurations described below are necessarily essential to the present invention.

[0009] The cellulose saccharification method according to this embodiment includes the steps of introducing a raw material containing cellulose and a reaction solution into a saccharification tank, introducing an enzyme into the reaction solution in the saccharification tank, placing an adsorption sheet on the surface of the reaction solution, and stirring the reaction solution to generate sugar in the reaction solution.

[0010] 1. Process of introducing raw materials and reaction solution into the saccharification tank. In this process, the raw material containing cellulose and the reaction solution are introduced into the saccharification tank.

[0011] 1.1. Saccharification tank The saccharification tank is not particularly limited as long as it can introduce raw materials and reaction solutions and stir the contents. The size of the saccharification tank is also not limited; it may be laboratory-scale using beakers and flasks, pilot plant-scale, or even commercial plant-scale.

[0012] The saccharification tank may include a container and a lid. The saccharification tank may also be equipped with an inlet for raw materials and reaction liquid, an outlet for the product, a mechanism for stirring the inside, a window for internal observation, a heater for heating and cooling, refrigerant piping, a jacket, and other piping as appropriate. Furthermore, the saccharification tank may be equipped with a liquid level gauge, a thermometer, etc., and may have openings for the installation of these.

[0013] Examples of stirring mechanisms include magnetic stirrs and stirring bars, stirring motors, shafts, and stirring blades, which can be appropriately selected depending on the scale and the stirring efficiency of the contents.

[0014] 1.2. Raw materials The raw materials introduced into the saccharification tank contain cellulose. The raw materials may also contain components other than cellulose. Examples of components other than cellulose include lignin and hemicellulose as wood-derived components, and fillers, pigments, resin components, clays, binders, toners, oils, and water as components of processed wood.

[0015] The raw materials may include paper, pulp, etc. Using paper as a raw material makes it easy to procure. The paper may also include recycled printed paper. Examples of recycled printed paper include copy paper, newspapers, and magazines. Using recycled printed paper is preferable because it saves environmental resources and landfill resources, and reduces waste.

[0016] The raw material may be introduced into the saccharification tank in a crushed state. The crushing may be carried out, for example, by cutting with a shredder or the like, or may be carried out by pulverization with a dry fiber disintegrator or the like. Further, the crushing may be carried out, for example, by wet dissociation. When the raw material is in a crushed state, it becomes easier to release components other than cellulose contained in the raw material from cellulose, and it becomes easier for cellulose to come into contact with the reaction solution, so that the efficiency of the saccharification process can be improved.

[0017] It is more preferable that the raw material is used in a sterilized state. Examples of the sterilization method include heating, ultraviolet irradiation, and the like. By doing so, glucose and the like generated by the saccharification reaction are less likely to be consumed by microorganisms derived from the raw material, etc., and the sugar yield may increase.

[0018] 1.3. Reaction Solution The reaction solution is mainly composed of water. In addition to water, the reaction solution may contain, for example, substances useful for enzyme reactions, pH adjusters, surfactants, and the like.

[0019] As the water, it is preferably pure water or ultrapure water such as ion-exchanged water, ultrafiltration water, reverse osmosis permeated water, distilled water, etc., with ionic impurities removed as much as possible. Also, when using water sterilized by ultraviolet irradiation or hydrogen peroxide addition, etc., it is preferable because it can suppress the generation of mold and bacteria during and after the enzyme reaction when storing the reaction solution for a long time.

[0020] Examples of the pH adjuster include one or more selected from organic acids such as acetic acid, citric acid, and phosphoric acid, inorganic acids, organic alkalis, inorganic alkalis, and salts such as their sodium salts, substances constituting buffer solutions, and the like.

[0021] Surfactants can be used without particular restrictions as long as they do not inhibit the enzymatic reaction. Examples of surfactants include TritonX-100 (manufactured by Nacalai Tesque Co., Ltd.), Antiform SE15 (manufactured by Sigma-Aldrich Japan LLC), Adekanol LG-126 (manufactured by ADEKA Corporation), DK Ester F-10 (manufactured by Daiichi Kogyo Seiyaku Co., Ltd.), and KM-72 (manufactured by Shin-Etsu Chemical Co., Ltd.). When saccharification raw materials such as dried recycled paper are mixed with the saccharification reaction solution, the saccharification reaction may be inhibited by the adhesion of air bubbles or other substances to the surface of the saccharification raw materials, but this inhibition of the saccharification reaction can be suppressed by adding a surfactant.

[0022] 1.4. Method of implementation There are no particular restrictions on the method of introducing the raw materials and reaction solution into the saccharification tank. Furthermore, the order in which they are introduced into the saccharification tank is also flexible; either the raw materials or the reaction solution may be introduced first, or they may be introduced simultaneously.

[0023] 2. Step of introducing the enzyme into the reaction solution. In this step, an enzyme is introduced into the reaction mixture. This step is performed at least before the saccharification reaction.

[0024] 2.1 Enzymes Any enzyme that breaks down cellulose into sugar by cleaving β-1,4-glucosidic bonds can be used. Examples of cellulose-degrading enzymes include endoglucanase, cellobiohydrolase, and cellobiase (β-glucosidase). More specific examples of cellulose-degrading enzymes include cellulase SS (manufactured by Nagase ChemteX Corporation), Accellerase Duet (manufactured by Genencor), Cellic Ctec2 (manufactured by Novozymes), Cellic Ctec3 (manufactured by Novozymes), and Meicerase (manufactured by Meiji Seika Pharma Co., Ltd.), and multiple types of these enzymes may be used in combination. In addition, xylanase may be included to simultaneously decompose xylan present on the cellulose surface and increase the efficiency of saccharification.

[0025] 2.2. Method of implementation The enzyme may be introduced into the reaction mixture as a solution or dispersion, or as a powder. The enzyme may be introduced after the raw materials and reaction mixture have been introduced into the saccharification tank, or it may be mixed with the reaction mixture or raw materials beforehand and introduced together with the reaction mixture or raw materials.

[0026] 3. Step of placing an adsorption sheet on the liquid surface in the saccharification tank. In this process, an adsorption sheet is placed on the liquid surface in the saccharification tank into which at least the raw materials and reaction solution have been introduced. The adsorption sheet may be fixed to the saccharification tank, or it may be moved within the saccharification tank by stirring as described later. Alternatively, the adsorption sheet may be placed on the liquid surface within the saccharification tank by buoyancy from the reaction solution, or it may be installed on the liquid surface using structural materials or the like.

[0027] 3.1. Adhesive Sheet The adsorption sheet adsorbs oily impurities such as lignin and pitch in the raw materials. The adsorption sheet may adsorb not only oily impurities such as lignin and pitch in the raw materials, but also oily impurities released from the raw materials by enzymatic reactions. The adsorption sheet can physically adsorb and collect oily impurities. The adsorption sheet does not need to cover the entire surface of the liquid in the saccharification tank; it is sufficient to place it on at least a portion of the liquid surface. Furthermore, one or more adsorption sheets may be placed on the liquid surface.

[0028] (Material of the adhesive sheet) The material of the adsorption sheet is not particularly limited, as long as it does not degrade during the enzymatic reaction and has an affinity for oil. In other words, the material of the adsorption sheet should be one that does not easily corrode at a pH near the optimal pH of the enzyme used in the enzymatic reaction and can adsorb or absorb oily components on its surface or internally. Examples of such materials include metals, carbon, inorganic materials, and resins that do not easily corrode at a pH near the optimal pH of the enzyme, as well as composite materials thereof. More specific materials include stainless steel, polyethylene, polypropylene, polytetrafluoroethylene, polyurethane, polyester, polybutadiene, styrene-butadiene rubber, silicone rubber, natural rubber, carbon fiber, activated carbon, etc., as well as composite materials of multiple materials thereof.

[0029] (Structure of the adhesive sheet) Adsorbent sheets adsorb oily components, and a larger surface area is preferable because it increases the efficiency of adsorption. From this viewpoint, it is preferable that the adsorbent sheet has a structure with a large specific surface area, such as a mesh structure, woven fabric structure, nonwoven fabric structure, sponge structure, or porous structure. Furthermore, if the adsorbent sheet has a mesh structure, the mesh opening may be, for example, 0.1 mm to 10 mm, preferably 0.5 mm to 5 mm, and more preferably 1 mm to 3 mm. In this way, liquid permeability of the adsorbent sheet can be obtained while improving the adsorption efficiency. As a result, even if the entire liquid surface is covered with the adsorbent sheet, for example, an enzyme solution can be introduced or added to the reaction solution through the mesh.

[0030] (The macroscopic shape of the adhesive sheet) The overall shape of the adsorption sheet is not particularly limited. The thickness of the adsorption sheet is, for example, 10 μm or more and 5 cm or less, preferably 100 μm or more and 3 cm or less, and more preferably 1 mm or more and 1 cm or less. The thickness of the adsorption sheet can be set to a degree that maintains its shape or stiffness, depending on the material and structure of the adsorption sheet, and in this way the handling of the adsorption sheet can be made easier.

[0031] The shape of the adsorption sheet when viewed from above is not particularly limited. The adsorption sheet may have holes, notches, or cutouts when viewed from above. The shape of the adsorption sheet when viewed from above can be appropriately designed depending on the shape of the saccharification tank, the supply and addition of raw materials and enzymes, the configuration of the stirring mechanism, the handling of the adsorption sheet, the cleaning of the saccharification tank, and other factors.

[0032] If the saccharification tank has a cylindrical shape and the liquid surface inside the tank is circular in plan view, it is more preferable that the adsorption sheet has an annular shape in plan view. In this case, the shaft of the stirring blade can be passed through the hole in the center of the annular shape of the adsorption sheet, and additional components can be introduced into the saccharification tank, thereby further increasing the saccharification efficiency.

[0033] 3.2. Variations in arrangement It is more preferable to position the adsorption sheet close to the inner wall of the saccharification tank. This allows the adsorption sheet to be placed near the inner wall of the saccharification tank, where oily impurities such as lignin and pitch in the raw materials tend to accumulate due to stirring. This makes the collection of impurities by the adsorption sheet more efficient.

[0034] 4. Process for producing sugar In this step, the reaction mixture is stirred, and the enzyme breaks down the cellulose, generating cellulose-derived sugars in the reaction mixture. This step takes place for a period of 2 hours to 1 week, depending on the enzyme's capacity and the overall scale of the process. Typically, this step takes between 10 hours and 5 days, more preferably between 1 and 4 days.

[0035] 4.1. Stirring Agitation is performed using the appropriate agitation mechanism described above. The motor rotation speed and other parameters during agitation can be appropriately set according to the size and configuration of the saccharification tank, the shape of the stirring bar or stirring blades, and the type of sugar.

[0036] 4.2. pH The pH of the reaction solution in this process is adjusted according to the optimal pH of the enzyme being introduced. For example, if the enzyme used is Cellic Ctec2 (manufactured by Novozymes), the pH of the liquid in the saccharification tank should be between 4.5 and 6, preferably between 5.0 and 5.7. It is more preferable to adjust the pH before introducing the enzyme into the saccharification tank.

[0037] The pH can be adjusted by adding sodium acetate, acetic acid, sulfuric acid, or an aqueous solution of sodium hydroxide to the reaction solution. Alternatively, if the reaction solution's pH can be monitored, the pH may be adjusted during stirring. For example, if the pH becomes higher than a predetermined level during stirring, acetic acid or sulfuric acid can be added; if the pH becomes lower than a predetermined level, an aqueous solution of sodium hydroxide can be added to adjust it.

[0038] Furthermore, if the adsorption sheet described above has holes, acids, alkalis, etc., for pH adjustment can be more easily introduced into the liquid through these holes.

[0039] 4.3.Temperature In this process, the temperature of the liquid in the saccharification tank is preferably adjusted to the optimal temperature of the enzyme used. For example, if the enzyme used is Cellic Ctec2 (manufactured by Novozymes), the temperature of the liquid in the saccharification tank is 47°C to 52°C, preferably 48°C to 51°C, and more preferably 49°C to 50°C.

[0040] The temperature of the liquid in the saccharification tank is controlled using appropriate heating devices, cooling devices, control devices, etc.

[0041] 5. Other processes The saccharification method of this embodiment may include, in addition to the above steps, a pretreatment step, a recovery step, a washing step, and so on.

[0042] 5.1. Pretreatment process The saccharification method may include a pretreatment step. Examples of pretreatment steps include sterilizing the raw materials and reaction solution, grinding the raw materials, and classifying the raw materials.

[0043] 5.2. Recovery Process The saccharification method may include a step of recovering the liquid in the saccharification tank after the step of generating the sugar described above. The recovery step may be carried out through piping or the like. The recovered liquid containing the sugar may be filtered. Used adsorbent sheets may also be recovered during the recovery step.

[0044] 5.3. Cleaning of the saccharification tank The saccharification method may include a step of cleaning the saccharification tank after the step of producing the sugar described above. The step of cleaning the saccharification tank may include, for example, a step of removing the precipitate remaining in the saccharification tank, a step of cleaning any oil remaining in the saccharification tank, a step of cleaning the stirring mechanism, etc., before and / or after the recovery step.

[0045] 6. Effects According to the saccharification method of this embodiment, oily impurities such as lignin and pitch in the raw materials can be captured by the adsorption sheet, thus reducing the inhibition of the enzymatic reaction and making it easier to maintain cleanliness inside the saccharification tank. As a result, cellulose can be saccharified efficiently, and sugar can be produced efficiently. Furthermore, because the inside of the saccharification tank is cleaner, cleaning the saccharification tank after use is easier.

[0046] 7. Examples of experiments, etc. The present invention will be described in more detail below with reference to experimental examples, but the present invention is not limited to these examples.

[0047] 7.1. Specific Examples of Saccharification Reservoirs Figure 1 is a schematic diagram showing an overview of the saccharification process according to the example. Figure 1 shows the process of producing sugar derived from cellulose. Liquid L, which is a mixture of reaction solution, raw materials and enzymes, is introduced into the saccharification tank 100, and an adsorption sheet 30 is placed on the surface of liquid L. The stirring shaft 20 and stirring blades 10 are rotated by a motor (not shown) to stir the liquid L (raw materials, reaction solution, enzymes, etc.).

[0048] Through stirring, cellulose in liquid L is saccharified by an enzymatic reaction. Furthermore, oily impurities such as lignin and pitch introduced from the raw materials are collected on the adsorption sheet 30 through stirring. Additionally, oily impurities such as lignin and pitch released from the cellulose as the enzymatic reaction progresses are adsorbed onto the adsorption sheet 30.

[0049] 7.2. Examples of adhesive sheet shapes Figure 2 is a schematic diagram of the adsorption sheet 30 according to the embodiment, viewed in plan. As shown in the figure, the adsorption sheet 30 has a circular outer shape in plan view and an annular shape with a circular hole 31 in the center. Although not shown in the figure, the outer shape in plan view is not limited to a circle and can be freely set by the shape of the inner wall surface of the saccharification tank 100, etc. Furthermore, the hole 31 in plan view is not limited to being formed in the center, and the shape of the hole 31 is arbitrary. Moreover, multiple holes 31 may be formed. The adsorption sheet 30 has holes 31 formed therein, which allows each main component to be supplied to the liquid L through the holes 31. In addition, the holes 31 allow stirring operations to be performed without interference between the stirring shaft 20 and the adsorption sheet 30.

[0050] Figure 3 is a schematic diagram of the adsorption sheet 32 ​​according to a modified embodiment, viewed in plan. As shown in the figure, the adsorption sheet 32 ​​has a circular outer shape in plan view, an annular shape with a circular hole 31 in the center, and a notch 33 is formed connecting the outer circumference and the hole 31. The outer shape and hole 31 of the adsorption sheet 32 ​​are the same as those of the adsorption sheet 30 described above. When a notch 33 is formed as in the adsorption sheet 32, it becomes easy to place the adsorption sheet 32 ​​on the surface of the liquid L, for example, even after the stirring shaft 20 has been installed in a predetermined position.

[0051] The inventors have found that impurities tend to float near the inner wall surface during stirring. Therefore, as shown in the examples in Figures 1 to 3, by placing adsorption sheets 30 and 32 near the inner wall surface of the saccharification tank 100, it becomes easier to capture oily impurities such as lignin and pitch.

[0052] 7.3. Experimental Examples The effect of an adsorption sheet on the efficiency of cellulose saccharification was investigated through the following experiment. Two sets of a 2L beaker were prepared, each containing 50g of recycled paper, 1L of sodium acetate buffer, and the enzyme Cellic Ctec2 (Novozymes). Acetic acid was added to adjust the pH to 5.2. One set contained a magnetic stirrer rotor and an adsorption sheet (material: silicone rubber, thickness: 5mm, shape: cut to fit the inner wall of the beaker). The other set contained only the magnetic stirrer rotor. Both sets were then stirred for two days at a temperature of 49°C.

[0053] The liquids obtained after the reaction were collected, and the difference in sugar concentration with and without the adsorption sheet was compared. The experimental method was as follows: (1) Prepare multiple reference sugar solutions with known sugar concentrations. (2) To the reference sugar solution prepared in (1) and 0.02 mL of the saccharification reaction solution diluted to a sugar solution concentration of 50 mg / dLL to 500 mg / dL or less, add 3 mL of the sugar solution concentration measuring reagent (product name "Glucose CII-Test Wako", manufactured by Fujifilm Wako Pure Chemical Corporation) by pipetting and stirring. (3) Let the solution from (2) stand at 37°C for 5 minutes. (4) The wavelength of the liquid color is measured by absorbance measurement. (5) Draw a calibration curve from the reference to the absorbance obtained at a wavelength of 505 nm and calculate the sugar solution concentration of the saccharification reaction solution.

[0054] The results of the above experiment showed that when the adsorbent sheet was placed, a sugar concentration of 30 g / L was obtained, and the sugar production rate (saccharification rate) relative to the total amount of cellulose was 60%. In contrast, when the adsorbent sheet was not placed, a sugar concentration of 23 g / L was obtained, and the sugar production rate (saccharification rate) relative to the total amount of cellulose was 45%. Thus, a remarkable effect was confirmed in that the saccharification rate improved by 15% when the adsorbent sheet was placed.

[0055] The embodiments and variations described above are merely examples and are not limited thereto. For example, each embodiment and each variation can be combined as appropriate.

[0056] The present invention includes configurations substantially identical to those described in the embodiments, for example, configurations with the same function, method, and results, or configurations with the same purpose and effect. Furthermore, the present invention includes configurations in which non-essential parts of the configurations described in the embodiments are replaced. Furthermore, the present invention includes configurations that produce the same effects or achieve the same purpose as those described in the embodiments. Finally, the present invention includes configurations that add known technology to the configurations described in the embodiments.

[0057] The following can be derived from the embodiments and modifications described above.

[0058] Methods for saccharifying cellulose are: The process involves introducing a raw material containing cellulose and a reaction solution into a saccharification tank. The steps include introducing an enzyme into the reaction solution in the saccharification tank, The steps include: placing an adsorbent sheet on the surface of the reaction solution; The process involves stirring the reaction solution and generating sugar by decomposing the cellulose with the enzyme, Includes, The adsorption sheet adsorbs oily components that are released from the raw materials to the surface of the reaction solution during the sugar production process.

[0059] This cellulose saccharification method allows impurities such as lignin and pitch, which are oily components in the raw material, to be captured by an adsorption sheet. This reduces the likelihood of enzyme reactions being inhibited and makes it easier to maintain a clean saccharification tank. As a result, cellulose can be saccharified efficiently, leading to efficient sugar production. Furthermore, because the saccharification tank remains cleaner, cleaning the tank after use is easier.

[0060] In the above method for saccharifying cellulose, The aforementioned raw material may be paper.

[0061] This method of saccharifying cellulose allows for easy procurement of raw materials.

[0062] In the above method for saccharifying cellulose, The aforementioned paper may include recycled paper that has already been printed.

[0063] This cellulose saccharification method allows for the efficient extraction of sugar from waste paper that has previously been treated as waste, thus effectively addressing the conservation of environmental and landfill resources. Furthermore, since printed waste paper contains oils such as ink, these oils can be effectively removed using an adsorbent sheet, resulting in a more significant improvement in saccharification efficiency and a more significant simplification of cleaning the saccharification tank.

[0064] In the above method for saccharifying cellulose, The raw materials may be introduced into the saccharification tank in a coarsely crushed state.

[0065] This cellulose saccharification method makes it easier to release unwanted components contained in the raw material and allows the cellulose to come into contact with the reaction solution, thereby further improving the saccharification efficiency.

[0066] In the above method for saccharifying cellulose, The adsorption sheet may have a mesh structure.

[0067] This cellulose saccharification method allows for easier capture of oily impurities such as lignin and pitch in the raw material using an adsorption sheet, thereby further increasing the saccharification efficiency.

[0068] In the above method for saccharifying cellulose, The suction sheet may have an annular shape in a plan view.

[0069] This cellulose saccharification method allows the shaft of the stirring blade to pass through the central hole in the ring of the adsorption sheet, and additional components to be introduced into the saccharification tank, thereby further increasing the saccharification efficiency.

[0070] In the above method for saccharifying cellulose, The adsorption sheet may be positioned close to the inner wall of the saccharification tank.

[0071] According to this cellulose saccharification method, the adsorption sheet is placed near the inner wall of the saccharification tank where oily impurities such as lignin and pitch in the raw material tend to accumulate, thus making the collection of impurities by the adsorption sheet more efficient. [Explanation of Symbols]

[0072] 10…Agitation blade, 20…Agitation shaft, 30,32…Adsorption sheet, 31…Hole, 33…Notch, 100…Saccharification tank, L…Liquid

Claims

1. The process involves introducing a raw material containing cellulose and a reaction solution into a saccharification tank. The steps include introducing an enzyme into the reaction solution in the saccharification tank, The steps include: placing an adsorbent sheet on the surface of the reaction solution; The process involves stirring the reaction solution with a stirring blade and generating sugar by decomposing the cellulose with the enzyme, Includes, The adsorption sheet is provided separately from the stirring blade and is fixed to the saccharification tank, and in the process of producing the sugar, it is released from the raw materials to the surface of the reaction solution. A method for saccharifying cellulose to adsorb oily components.

2. In claim 1, The aforementioned raw material is paper, and the method is for saccharifying cellulose.

3. In claim 2, The aforementioned paper includes printed waste paper, and the method is for saccharifying cellulose.

4. In any one of claims 1 to 3, A method for saccharifying cellulose, wherein the raw material is introduced into the saccharification tank in a coarsely crushed state.

5. In any one of claims 1 to 4, The adsorption sheet has a network structure, and this is a method for saccharifying cellulose.

6. In any one of claims 1 to 5, The adsorption sheet has an annular shape in plan view, and this is a method for saccharifying cellulose.

7. In any one of claims 1 to 6, A method for saccharifying cellulose, wherein the adsorption sheet is placed in close proximity to the inner wall of the saccharification tank.

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