Genome extraction device including flow cover

Abstract

The present invention relates to a genome extraction device including a flow cover, and by including a base plate, provides a genome extraction device that forms a sealed flow path inside while preventing the problem of the flow path being narrowed due to tight engagement between components.

Description

Technical Field

[0001] The present invention relates to a genomic extraction device including a flow cover.

Background Art

[0002] In modern times, with the progress of biotechnology, it has become possible to interpret the causes of diseases at the gene level. As a result, the requirements for the manipulation of biological samples and biochemical analysis for curing or preventing human diseases have been gradually increasing.

[0003] In addition to disease diagnosis, technologies for extracting and analyzing nucleic acids from samples containing biological samples or cells are required in various fields such as new drug development, pre-examination for the presence or absence of virus or bacterial infections, and forensic medicine.

[0004] Conventional genomic extraction devices require separate devices for each processing step (concentration, purification). After one processing step is completed, it must be transferred to another device, which requires a long time.

[0005] In order to solve the conventional problem of low detection efficiency in such a long processing process, a registered patent No. 10-1989920 by the present applicant has been developed and used.

[0006] In the above literature, the buffer is directly dispensed and stored inside the buffer chamber. However, during long-term storage, fine leakage occurs through various layer structures at the bottom of the buffer chamber, which has a problem of affecting the extraction performance.

[0007] Also, the pad disposed between the upper body and the base plate is formed of a rubber material. However, when the pad is crimped between the upper body and the base plate, the diameter of the hole formed through the pad decreases, resulting in a problem that an extraction liquid with a capacity different from the intention of the product design moves to the amplification module.

[0008] Furthermore, vibrations generated during product production and distribution could cause the sealing member that seals the buffer chamber to be perforated by the protruding member, leading to the leakage and contamination of reagents stored inside the buffer chamber.

[0009] Therefore, the inventors focused on and completed the present invention in order to solve the problems of conventional genome extraction devices. [Prior art documents] [Patent Documents]

[0010] [Patent Document 1] Korean Registered Patent Document No. 10-1989920 [Patent Document 2] Korean Registered Patent Document No. 10-2065649 [Patent Document 3] Korean Registered Patent Document No. 10-2065650 [Patent Document 4] Korean Registered Patent Document No. 10-2076220 [Overview of the Initiative] [Problems that the invention aims to solve]

[0011] The present invention aims to provide a genome extraction apparatus that solves the problem of reagents contained in a single chamber leaking out in conventional genome extraction apparatuses, by providing an inner chamber containing reagents necessary for genome extraction separately from the outer chamber, and by sealing the upper and lower parts of the inner chamber.

[0012] Furthermore, the objective is to provide a genome extraction device that includes safety clips to prevent the sealing members that seal the upper and lower openings of the inner chamber from being perforated by the protruding members formed on the cover and outer chamber, due to the movement of the inner chamber up and down caused by vibrations that occur during the production and distribution process of the product.

[0013] Furthermore, the objective is to provide a genome extraction device that solves the problem of cross-contamination between reagents due to capillary action occurring through the space between the double chambers, through a unique inner chamber design (lower inner chamber).

[0014] Furthermore, the objective is to provide a genome extraction device that solves the problem of reagent leakage to the outside through a unique inner chamber design (upper inner chamber) in a structure that prevents capillary action.

[0015] Furthermore, the configuration of the first protruding member formed on the bottom surface of the outer chamber allows the sealing member to be broken with little force, the perforated portion to expand, and the goal is to provide a genome extraction device that allows reagents contained inside the inner chamber to flow out smoothly to the outside.

[0016] Furthermore, the objective is to provide a genome extraction device in which a sloping portion is formed around the discharge port through which the reagent is discharged, thereby enabling the reagent to be discharged smoothly through the discharge port.

[0017] Furthermore, by arranging a double-layered flow cover-pad between the outer chamber and the base plate, the aim is to provide a genome extraction device that offers improved manufacturing convenience compared to conventional genome extraction devices that only have one pad, and solves the problem of unintentionally narrowing the flow path.

[0018] Furthermore, the objective is to provide a genome extraction device in which a sealed channel is formed without the phenomenon of leakage during the process of reagent movement, by achieving a strong engagement between the base plate, flow cover, pad, and outer chamber.

[0019] Furthermore, the bead chamber, which contains the beads necessary for genome extraction and amplification, has a double-chamber structure consisting of an outer chamber and a bead chamber. This aims to provide a genome extraction device that can maintain the performance of moisture-sensitive beads for extended periods.

[0020] Another object is to provide a genomic extraction device that maintains the performance of beads by a dehumidifying part located above the bead chamber even when the bead chamber is opened.

[0021] Another object is to provide a genomic extraction device to which an amplification module is applied, in which air remaining inside the storage part is easily discharged as a pre-treated extraction liquid is introduced, so that an extraction liquid with a sufficient volume can be introduced.

[0022] Another object is to provide a genomic extraction device in which the amplification module has a plurality of storage parts, primers and probes for different genomic amplifications are stored in each storage part, and several types of disease diagnoses are possible through one genomic extraction.

[0023] Another object is to provide a genomic extraction device that is provided such that the lengths, thicknesses, and patterns of the gas movement passage and the extraction liquid movement passage are different from each other depending on the positions of the connected storage parts, preventing the extraction liquid or amplification products introduced into the storage part from mixing.

[0024] Another object of the present invention is to provide a genomic extraction method using the above-described genomic extraction device.

Means for Solving the Problems

[0025] One embodiment of the present invention for solving the above problems is an outer chamber partitioned internally into a plurality of first spaces by an outer chamber partition wall, with different reagents accommodated in each of the plurality of first spaces, an outer chamber, a base plate engaged with the lower part of the outer chamber, the base plate having a plurality of flow paths formed on the upper surface and communicating with the plurality of first spaces, a flow cover disposed between the outer chamber and the base plate, with flow cover holes penetrating therethrough to connect the plurality of first spaces and the plurality of flow paths, and a pad disposed above the flow cover, with pad holes penetrating therethrough to connect the flow cover holes and the plurality of first spaces, providing an extraction device.

[0026] In one embodiment, the flow cover may be made of a plastic material, and the pad may be made of a silicon material.

[0027] In one embodiment, the plurality of flow paths extend radially outward from a portion of the base plate that is a predetermined distance away from the center of the base plate, one end is disposed on a first circumference that is a first distance away from the center of the base plate, a part of the plurality of flow paths has the other end disposed on a second circumference that is a second distance away from the center of the base plate, and another part of the plurality of flow paths has the other end disposed on a third circumference that is a third distance away from the center of the base plate and is longer than the second distance.

[0028] In one embodiment, the plurality of flow paths may further include an air flow path where one end is disposed at a distance away from the center of the base plate that is longer than the first distance and shorter than the second distance, and the other end is disposed at a distance away from the center of the base plate that is longer than the second distance and shorter than the third distance.

[0029] In one embodiment, the flow cover has a first through hole in which a driving part is installed, and first engaging protrusions protruding upward and downward are formed on the outer periphery of the first through hole. The first engaging protrusions may be inserted into the driving part insertion hole of the base plate and the second through hole of the pad.

[0030] In one embodiment, the flow cover may have a plurality of first flow cover holes formed through a first circumference at a first distance from the first through hole, a plurality of second flow cover holes formed through a second circumference at a second distance from the first through hole, a plurality of third flow cover holes formed through a third circumference at a third distance from the first through hole, and a fourth flow cover hole formed through which communication occurs between one end and the other end of the air passage.

[0031] In one embodiment, molten projections that engage along the edges of the multiple flow channels may be formed on the bottom surface of the flow cover.

[0032] In one embodiment, the pad may have a plurality of first pad holes formed through a first circumference at a first distance from the second through hole, a plurality of second pad holes formed through a second circumference at a second distance from the second through hole, a plurality of third pad holes formed through a third circumference at a third distance from the second through hole, and a fourth pad hole formed through which communication occurs between one end and the other end of the air passage.

[0033] In one embodiment, the diameter of the third pad hole may be larger than the diameters of the first pad hole and the second pad hole.

[0034] In one embodiment, a portion may be included that protrudes from the upper surface of the pad while being connected to the upper part of the third pad hole, and that widens towards the flow cover.

[0035] In one embodiment, the plurality of flow paths may be asymmetrical on a cross-section including the center of the base plate.

[0036] In one embodiment, the flow cover may be fused and fixed onto the base plate.

[0037] In one embodiment, one end of the plurality of flow paths is aligned with the first flow cover hole and the first pad hole; the other end of some of the flow paths in the plurality of flow paths is aligned with the second flow cover hole and the second pad hole; the other end of some of the other flow paths in the plurality of flow paths is aligned with the third flow cover hole and the third pad hole; and the air flow path is aligned with the fourth flow cover hole and the fourth pad hole.

[0038] In one embodiment, one end of the plurality of flow paths communicates with a fluid storage section inside a piston installed in the outer chamber, and the other end of the plurality of flow paths can communicate with discharge holes or amplification modules of a plurality of first spaces in the outer chamber. [Effects of the Invention]

[0039] The genome extraction apparatus according to the present invention is equipped with an inner chamber containing reagents necessary for genome extraction, separate from the outer chamber, and the upper and lower parts of the inner chamber are sealed, thereby solving the problem in conventional genome extraction apparatuses where reagents contained in a single chamber leak out.

[0040] Furthermore, vibrations generated during the production and distribution process of the product cause the inner chamber to move up and down, preventing the sealing members that seal the upper and lower openings of the inner chamber from being perforated by the protruding members formed on the cover and outer chamber.

[0041] Furthermore, the problem of cross-contamination between reagents is resolved by the capillary action that occurs through the space between the double chambers.

[0042] Furthermore, the structure designed to prevent capillary action prevents reagents from leaking out.

[0043] Furthermore, the configuration of the protruding member formed on the bottom surface of the outer chamber allows the sealing member to be broken with little force, the perforated portion expands, and the reagent contained inside the inner chamber flows out smoothly to the outside.

[0044] Furthermore, an inclined section is formed around the discharge port through which the reagent is discharged, allowing the reagent to be discharged smoothly through the port.

[0045] Furthermore, the placement of a double-layered flow cover-pad between the outer chamber and the base plate improves manufacturing convenience and solves the problem of unintentionally narrowing the flow path compared to conventional genome extraction devices that only have one pad.

[0046] Furthermore, the strong engagement between the base plate, flow cover, pad, and outer chamber creates a sealed channel without any leakage during the reagent's movement.

[0047] Furthermore, the bead chamber, which contains the beads necessary for genome extraction and amplification, has a double-chamber structure consisting of an outer chamber and a bead chamber, making it possible to maintain the performance of the moisture-sensitive beads for extended periods.

[0048] Furthermore, even if the bead chamber is opened, the performance of the bead is maintained by the dehumidifying unit located at the top of the bead chamber.

[0049] Furthermore, by introducing the pre-treated extract, residual air inside the containment section is easily removed, and a sufficient volume of extract is introduced into the amplification module.

[0050] Furthermore, the amplification module has multiple storage compartments, each containing different primers and probes for genome amplification, allowing for the diagnosis of several diseases through a single genome extraction.

[0051] Furthermore, depending on the location of the connected containment sections, the length, thickness, and pattern of the gas transport passage and the extract transport passage are provided to differ from each other, thereby preventing the mixing of the extract or amplification product introduced into the containment section. [Brief explanation of the drawing]

[0052] [Figure 1] This is a perspective view showing the overall configuration of a genome extraction apparatus according to an embodiment of the present invention. [Figure 2] Figure 1 is a perspective view of the genome extraction device from another side. [Figure 3] Figure 1 is an exploded perspective view. [Figure 4] This is a diagram illustrating the engagement relationship between the outer chamber and the inner chamber. [Figure 5] This is a diagram illustrating the engagement relationship between the inner chamber and the safety clip. [Figure 6] This is a plan view of the outer chamber. [Figure 7] This is a cross-sectional view illustrating the engagement relationship between the inner chamber and the outer chamber. [Figure 8] This is an enlarged drawing illustrating the protruding member formed on the bottom surface of the outer chamber. [Figure 9] This is a diagram to explain the inner chamber in more detail. [Figure 10] This is a bottom perspective view to provide a more detailed explanation of the cover. [Figure 11] This is an exploded perspective view to more specifically explain the flow cover and pad located between the base plate and the outer chamber. [Figure 12] This is an exploded perspective view to specifically explain the piston's configuration. [Figure 13] This is a bottom perspective view of the flow cover. [Figure 14] This is a perspective view to provide a more detailed explanation of the base plate. [Figure 15] This is a cross-sectional view illustrating a genome extraction device according to an embodiment of the present invention. [Figure 16] This is another cross-sectional view for specifically illustrating a genome extraction device according to an embodiment of the present invention. [Figure 17] This is a drawing illustrating an amplification module according to a first embodiment of the present invention. [Figure 18] This is a drawing illustrating an amplification module according to a first embodiment of the present invention. [Figure 19] This is a drawing illustrating an amplification module according to a first embodiment of the present invention. [Figure 20] This is a drawing illustrating an amplification module according to a second embodiment of the present invention. [Figure 21] This is a drawing illustrating an amplification module according to a second embodiment of the present invention. [Figure 22] This is a drawing illustrating an amplification module according to a second embodiment of the present invention. [Figure 23] This is a drawing illustrating an amplification module according to a third embodiment of the present invention. [Figure 24] This is a drawing illustrating an amplification module according to a third embodiment of the present invention. [Figure 25] This is a drawing illustrating an amplification module according to a third embodiment of the present invention. [Figure 26] This is a plan view of the bead chamber. [Figure 27] This is a perspective view to explain the bead chamber configuration in more detail. [Figure 28] This is a perspective view to explain the bead chamber configuration in more detail. [Figure 29] Figure 28 is a cross-sectional view of the bead chamber. [Figure 30] Figure 28 is a longitudinal cross-sectional view of the bead chamber, illustrating the structure in which it engages with the outer chamber. [Modes for carrying out the invention]

[0053] In some cases, known structures and devices are omitted or shown in the form of block diagrams focusing on the core function of each structure and device, in order to avoid obscuring the concept of the present invention.

[0054] Throughout the specification, when a part "comprising" or "including" a component, this means, unless otherwise specified, that it further includes other components, not excludes them. Furthermore, terms such as "...part," "...unit," and "module" as used in the specification mean a unit that processes at least one function or operation, which may be embodied in hardware, software, or a combination of hardware and software. Also, "a or an," "one," "the," and similar related terms are used in the context describing the invention (particularly in the context of the following claims) to include both singular and plural meanings unless otherwise specified herein or clearly contradicted by the context.

[0055] In describing embodiments of the present invention, if a specific description of a known function or configuration is deemed to unnecessarily obscure the gist of the invention, such detailed description will be omitted. Furthermore, the terms described later are defined in consideration of the functions in embodiments of the present invention, and these may change depending on the intent or conventions of the user or operator. Therefore, their definitions must be based on the overall content of this specification.

[0056] The present invention will be described in detail below with reference to the attached drawings.

[0057] Referring to Figures 1 and 2, the genome extraction apparatus 1000 according to an embodiment of the present invention includes an outer chamber 100, an inner chamber 200, a cover 300, a base plate 400, a safety clip 500, an amplification module 600, a piston 700, a drive unit 800, and a bead chamber 900.

[0058] The outer chamber 100 is divided into a plurality of first spaces 101, 102, 103, 104, 105, 106, and 107 by the outer chamber partition wall. That is, the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107 may be independent spaces from one another.

[0059] Multiple first spaces 101, 102, 103, 104, 105, 106, and 107 may be provided with an open top and a closed bottom. On the other hand, first discharge holes 121, 122, 123, 124, and 125 are formed through the bottom surfaces of the multiple first spaces 101, 102, 103, 104, and 105, and are formed along the circumference at a distance of one distance from the center of the outer chamber 100. Second discharge holes 126 and 127 are formed through the bottom surfaces of the remaining first spaces 106 and 107, and are formed along the circumference at a distance of two distance from the center of the outer chamber 100. In addition, discharge holes 128 and 129 communicating with the amplification module 600 are formed through the bottom surface of the space between the first spaces 106 and 107. Here, the first distance may be shorter than the second distance, but in other embodiments, the first distance may be even longer than the second distance.

[0060] Multiple first spaces 101, 102, 103, 104, and 105 are filled with reagents stored in the inner chamber 200, which will be described later, while the remaining multiple first spaces 106 and 107 are filled with beads stored in the bead chamber 900.

[0061] In the center of each of the multiple first spaces 101, 102, 103, 104, 105, 106, and 107, a piston insertion section 108 is formed vertically through which a piston 700 is inserted. When the piston 700 is inserted into the piston insertion section 108, and the drive unit of the diagnostic device (not shown) engages with the piston 700 to raise and lower the piston 700, the reagent (fluid) in the first spaces 101, 102, 103, 104, 105, 106, and 107 can enter and exit the fluid storage section 701 inside the piston 700. More specific details will be described later.

[0062] Referring to Figure 4, the upper outer surface 100a of the outer chamber 100 is recessed toward the center while being connected to the upper part of the lower outer surface 100b. The safety clip 500 engages with the upper outer surface 100a of the outer chamber 100, and the boundary between the upper outer surface 100a and the lower outer surface 100b acts as a short jaw of the safety clip 500, so that the engagement position of the safety clip 500 is maintained after it engages with the upper outer surface 100a. The safety clip 500 has a length that encloses at least a portion of the perimeter of the upper outer surface 100a of the outer chamber 100 and includes an extending outer chamber engaging portion 510 and a handle 520 formed on one side of the outer chamber engaging portion 510.

[0063] The safety clip 500 engages with the outer chamber 100, which can prevent the cover 300 from pressurizing the inner chamber 200 engaged with the outer chamber 100 and opening the upper and lower openings of the inner chamber 200. The user can remove the safety clip 500 from the outer chamber 100 by grasping the handle 520 and then start the extraction process. In other words, when the safety clip 500 is engaged with the outer chamber 100, the reagent in the inner chamber 200 cannot be introduced into the outer chamber 100, and only after the safety clip 500 is removed from the outer chamber 100 can the reagent in the inner chamber 200 be introduced into the outer chamber 100.

[0064] Refer to Figures 3-5 for a more detailed explanation of the 500 safety clip configuration.

[0065] The safety clip 500 includes an outer chamber engaging portion 510, a handle 520, an upper extension portion 530, and a side extension portion 540.

[0066] The outer chamber engaging portion 510 engages with the outer chamber 100, enclosing at least a portion of the outer surface of the outer chamber 100 (specifically, the upper outer surface 100a). More specifically, the outer chamber engaging portion 510 engages with the outer chamber 100 so as to enclose four outer surfaces of the outer chamber 100, although the extended ends of the outer chamber engaging portion 510 may be configured to be separated from each other. As shown in Figure 1, when the safety clip 500 engages with the outer chamber 100, the extended ends of the outer chamber engaging portion 510 grip one of the outer surfaces of the outer chamber 100, and the safety clip 500 is separated from the outer chamber 100 only when the user grasps the safety clip 500 and applies an external force in one direction.

[0067] The handle 520 is the portion that extends outward from the outer chamber engagement portion 510 and is the portion that is grasped by the user in order to separate the safety clip 500 from the outer chamber 100.

[0068] The upper extension 530 extends upward from one side of the outer chamber engagement portion 510, and the side extension 540 extends from the upper extension 530 toward the center of the outer chamber 100.

[0069] The safety clip 500 according to an embodiment of the present invention is characterized in that a cover support member 541 is formed to protrude from the upper surface of the side extension 540, and an inner chamber engaging portion 542 is formed to protrude from the extended end of the side extension 540.

[0070] The cover support member 541 plays a role in preventing the protruding members 311, 312, 313, 314, 315, 316, and 317 formed on the bottom surface of the cover 300 from damaging (perforating) the first sealing member S1 that seals the upper openings of the multiple second spaces 201, 202, 203, 204, and 205 of the inner chamber 200 and the third sealing member S3 that seals the upper opening of the bead chamber 900 when the safety clip 500 engages with the outer chamber 100.

[0071] As shown in Figure 15, when the safety clip 500 engages with the outer chamber 100 and the inner chamber 200, the protruding members 311, 312, 313, 314, 315, 316, and 317 are blocked from contacting the first sealing member S1 and the third sealing member S3. Therefore, when the safety clip 500 is engaged with the outer chamber 100 and the inner chamber 200, puncture of the inner chamber 200 and the bead chamber 900 is prevented, thereby preventing the reagents contained in the inner chamber 200 and the beads contained in the bead chamber 900 from flowing out into the outer chamber 100.

[0072] The inner chamber engaging portion 542 is the portion that engages with the fixing portion 230 of the inner chamber 200 when the safety clip 500 engages with the outer chamber 100. When the inner chamber engaging portion 542 engages with the fixing portion 230, the bottom surface of the inner chamber 200 is positioned at a predetermined distance from the bottom surface of the outer chamber 100, and therefore the second sealing member S2 that seals the lower openings of the multiple second spaces 201, 202, 203, 204, 205 is prevented from being torn by the protruding members 111, 112, 113, 114, 115 formed on the bottom surface of the outer chamber 100 (see Figure 15).

[0073] In the attached drawings, the inner chamber engaging portion 542 is shown in the form of an engaging projection and the fixing portion 230 is shown in the form of an engaging groove that engages with the engaging projection. However, in other embodiments, the inner chamber engaging portion 542 may be provided in the form of an engaging groove and the fixing portion 230 may be provided in the form of an engaging projection that engages with the engaging groove.

[0074] The outer chamber 100 (more specifically, the outer chamber partition wall) has a recessed attachment portion 109 that provides a space for the fixing portion 230 of the inner chamber 200 to be attached. The inner chamber 200 has a safety clip 500The outer chamber 100 is fixed at a predetermined distance from the bottom surface through an engagement structure, but the fixing force can be further improved by the fixing portion 230 of the inner chamber 200 being securely supported by the attachment portion 109.

[0075] Referring to Figure 7, an insertion space 130 is formed as a recess on the upper side of the inner wall of the outer chamber 100, and the engaging hook 240 of the inner chamber 200 can engage with the insertion space 130. Above the insertion space 130, a stopper 131 is formed to protrude toward the inside of the outer chamber 100. Therefore, when the inner chamber 200 is not pressurized by the cover 300, the engaging hook 240 of the inner chamber 200 is located on the stopper 131, but when the inner chamber 200 is pressurized by the cover 300, the engaging hook 240 may pass through the stopper 131 and be inserted into the insertion space 130.

[0076] Referring to Figure 15, the engagement relationship between the outer chamber and the inner chamber according to another embodiment of the present invention will be described. In Figure 7, instead of the engagement hook 240 formed on the inner chamber 200, a locking projection 250 is provided that protrudes outward from the outer wall of the inner chamber 200, and the locking projection 250 engages with a stopper 131 formed on the inner wall of the outer chamber 100, partially restricting the downward movement of the inner chamber 200. When the safety clip 500 is removed from the outer chamber 100 and the inner chamber 200 is pressurized by the cover 300, the locking projection 250 passes through the stopper 131 and is inserted into the insertion space 130, thereby perforating a second sealing member S2 in the inner chamber 200 that seals multiple second spaces with a protruding member formed on the outer chamber 100.

[0077] The inner chamber 200 is divided into multiple second spaces 201, 202, 203, 204, and 205 by the inner chamber partition wall. That is, the multiple second spaces 201, 202, 203, 204, and 205 may be independent spaces from one another.

[0078] The upper and lower ends of the multiple second spaces 201, 202, 203, 204, and 205 are open (i.e., the multiple second spaces have an upper opening and a lower opening), the upper end is sealed by the first sealing member S1 and the lower end is sealed by the second sealing member S2. The first sealing member S1 and the second sealing member S2 may be, for example, a film, but are not limited to this, and a film made of any material that does not allow fluid to pass through may be used.

[0079] Different reagents are placed in each of the multiple second spaces 201, 202, 203, 204, and 205. First, the second sealing member S2 seals the lower parts of the multiple second spaces, then the reagents are placed in, and finally the first sealing member S1 seals the upper parts of the multiple second spaces, thereby completing the placement of reagents into the inner chamber 200.

[0080] Referring to Figure 4, the inner chamber 200 includes an upper inner chamber 210 and a lower inner chamber 220.

[0081] The upper inner chamber 210 is integrally formed and is configured to be in close contact with the inner wall of the outer chamber 100 when engaged with it.

[0082] The lower inner chamber 220 is connected to the upper inner chamber 210 and includes a portion that is bent (radially inward) so as to separate from the inner wall of the outer chamber 100 when engaged with the outer chamber 100.

[0083] In this invention, a double-chamber structure consisting of an inner chamber and an outer chamber is used, which may pose a risk of cross-contamination between reagents in the inner chamber 200 during operation. Cross-contamination occurs due to capillary action through the microspace between the inner and outer chambers. In this invention, to prevent the aforementioned problem of cross-contamination, a structure is adopted in which the inner chamber 200 is bent so as to be sufficiently separated from the inner wall of the outer chamber 100, thereby preventing capillary action.

[0084] Furthermore, in order to prevent capillary action, the outer chamber 100 and the inner chamber 200 are separated, and to prevent reagents from flowing out through the separated portion, the upper inner chamber 210 is configured to be in close contact with the inner wall of the outer chamber 100.

[0085] On the other hand, second protruding members 111, 112, 113, 114, and 115 are formed on the bottom surfaces of the multiple first spaces 101, 102, 103, 104, and 105, which allow the reagent contained in the inner chamber 200 to flow out into the multiple first spaces 101, 102, 103, 104, and 105 by breaking the second sealing member S2 of the inner chamber 200.

[0086] Each of the second protruding members 111, 112, 113, 114, and 115 may be arranged in a one-to-one correspondence with a plurality of first spaces 101, 102, 103, 104, and 105. For example, the second protruding member corresponding to reference numeral 111 will break through the second sealing member S2 that seals the lower part of the second space corresponding to reference numeral 201, and the second protruding member corresponding to reference numeral 115 will break through the second sealing member S2 that seals the lower part of the second space corresponding to reference numeral 205.

[0087] The second protruding members 111, 112, 113, 114, 115 include protruding portions 111a, 112a, 113a, 114a, 115a that protrude by a first height (h1) from the bottom surface of a plurality of first spaces 101, 102, 103, 104, 105, and wing portions 111b, 112b, 113b, 114b, 115b that extend from the protruding portions 111a, 112a, 113a, 114a, 115a and protrude by a second height h2 lower than the first height h1 from the bottom surface. Here, the wing portions 111b, 112b, 113b, 114b, 115b may have a structure that extends from the protruding portions 111a, 112a, 113a, 114a, 115a in both left and right directions.

[0088] The protruding portion plays the role of perforating the second sealing member S2, and the wing portion plays the role of expanding the perforated portion of the second sealing member S2. In this invention, since the height of the protruding portion is higher than that of the wing portion, point contact occurs between the second sealing member S2 that seals the lower part of the inner chamber 200 and the protruding portion, and the pressure is minimized when the second sealing member S2 is torn through point contact. Therefore, the second sealing member S2 can be broken with less force.

[0089] When the second sealing member S2 is ruptured by the protruding members 111, 112, 113, 114, and 115, the reagents stored in the multiple second spaces 201, 202, 203, 204, and 205 of the inner chamber 200 flow out into the multiple first spaces 101, 102, 103, 104, and 105 of the outer chamber 100. The flowed-out reagents are then discharged through the first discharge holes 121, 122, 123, 124, and 125 formed on the bottom surface of the first spaces 101, 102, 103, 104, and 105. To facilitate the outflow of reagents to the first discharge holes 121, 122, 123, 124, and 125, there are inclined portions around the first discharge holes 121, 122, 123, 124, and 125 that slope downward toward the first discharge holes 121, 122, 123, 124, and 125. These inclined portions can have an angle of 3 to 10 degrees, which facilitates the process by which reagents that have flowed into the first spaces 101, 102, 103, 104, and 105 escape to the first discharge holes 121, 122, 123, 124, and 125.

[0090] The cover 300 engages with the top of the outer chamber 100 and is configured to cover the tops of the outer chamber 100 and the inner chamber 200.

[0091] Referring to Figure 10, the cover 300 includes a cover body 301 and a lid 302.

[0092] The cover body 301 has a first insertion hole 307 aligned with the piston insertion portion 108 and a first sample insertion hole 309 through which a sample is inserted (in the attached drawing, the first sample insertion hole 309 is aligned with the first space 105, and the sample is inserted into the first space 105 through the first sample insertion hole 309). The bottom surface of the cover body 301 has protruding first protruding members 311, 312, 313, 314, and 315 that break the first sealing member S1 and third protruding members 316, and 317 that break the third sealing member S3.

[0093] The first protruding members 311, 312, 313, 314, and 315 may be arranged in a one-to-one correspondence with a plurality of first spaces 101, 102, 103, 104, 105, 106, and 107, and the third protruding members 316 and 317 may be arranged in a one-to-one correspondence with a plurality of third spaces 910 and 920. For example, the first protruding member corresponding to reference numeral 311 will break through the first sealing member S1 that seals the upper part of the second space corresponding to reference numeral 201, and the first protruding member corresponding to reference numeral 315 will break through the first sealing member S1 that seals the upper part of the second space corresponding to reference numeral 205.

[0094] A separation member 320 is formed on the bottom surface of the cover body 301 along the periphery of the first insertion hole 307. The separation member 320 is the part that separates the first protruding member and the first sealing member from each other when the safety clip 500 is engaged with the outer chamber 100. That is, the cover 300 is separated from the inner chamber 100 by a predetermined distance as the separation member 320 is supported by the cover support member 541.

[0095] The lid 302 is hinged and rotatably connected to one side of the cover body 301. A second insertion hole 308 is formed through the center of the lid 302, aligned with the first insertion hole 307.

[0096] With the cover 300 engaged with the outer chamber 100, after separating the safety clip 500 from the outer chamber 100, pressing the cover 300 downward causes the inner chamber 200, which is engaged with the outer chamber 100, to descend along the inner wall of the outer chamber 100. Second protruding members 111, 112, 113, 114, 115, 116, and 117 are formed on the bottom surface of the outer chamber 100, and first protruding members 311, 312, 313, 314, and 315 and third protruding members 316 and 317 are formed on the bottom surface of the cover 300. The protruding members cause the first sealing member S1 and second sealing member S2, which seal the upper and lower openings of the inner chamber 200, and the third sealing member S3, which seals the upper opening of the bead chamber 900, to tear. Therefore, the reagent contained in the inner chamber 200 flows out into the multiple first spaces 101, 102, 103, 104, and 105 of the outer chamber 100, and the second sealing member S2 that seals the upper opening of the inner chamber 200 ruptures, acting as an air vent so that the reagent can be sufficiently discharged into the first spaces.

[0097] The base plate 400 engages with the lower part of the outer chamber 100 and includes multiple flow channels that guide the reagent's path between the first spaces 101, 102, 103, 104, 105, 106, 107 of the outer chamber 100 and the fluid reservoir of the piston 700.

[0098] According to one embodiment of the present invention, the base plate 400 has liquid channels 401 to 408 through which liquid can move and an air channel 409 through which air can move. Between the outer chamber 100 and the base plate 400, a flow cover 410 and a pad 420 can be further included, which are placed on the upper surface of the base plate 400 to prevent liquid leakage when the outer chamber 100 is engaged. When the base plate 400, flow cover 410, and pad 420 are engaged, the upper surfaces of the liquid and air channels of the base plate 400 are closed by the flow cover 410 and pad 420, forming a space and completing a flow path.

[0099] The liquid flow paths 401-408 are connected to the flow cover 410, pad 420, and outer chamber 100, providing a space in which samples and reagents can move and mix.

[0100] The air passage 409 connects the vacuum control section of the amplification module 600 and the piston 700, controlling the vacuum that may be generated when the genome extracted from the amplification module 600 moves, and preventing contamination of the amplification product that may be generated during genome amplification.

[0101] In one embodiment, one end of the air passage 409 is in communication with the fluid storage section 701 of the piston 700, and the other end is in communication with the amplification module 600, and the air discharged from the amplification module 600 may pass through the air passage 409 and be discharged into the fluid storage section 701.

[0102] Multiple channels 401, 402, 403, 404, 405, 406, 407, 408, and 409 are formed on the upper part of the base plate 400. Each channel does not intersect with the others. Base plate It is formed to extend from the center of 400 to the outer casing. Here, the liquid flow path corresponds to the configuration shown in reference numerals 401 to 408, and the air flow path corresponds to the configuration shown in reference numeral 409.

[0103] Referring to Figure 14, some of the multiple flow channels may have one end positioned on the same circumference, and the other ends may also be positioned on the same circumference as each other. Among the multiple flow channels, one end of the air flow channel 409 is located on a different circumference than one end of the other liquid flow channels 401-408, and the other end is also located on a different circumference than the other ends of the other liquid flow channels 401-408, making it possible to control the vacuum.

[0104] A piston drive unit insertion hole 400a is formed through the center of the base plate 400 so that the piston drive unit 800, which rotates the piston 700, engages with it.

[0105] A flow cover 410 is placed in the anchoring space on top of the base plate 400. The flow cover 410 may be made of plastic, for example, and may be ultrasonically fused to the top of the base plate 400 so as to be integral with the base plate 400.

[0106] The flow cover 410 has a first through hole 410a aligned with the insertion hole 400a of the piston drive unit, and a plurality of first flow cover holes 411a, 412a, 413a, 414a, 415a, 416a, 417a, 418a are formed through the first flow cover hole 411a, 412a, 413a, 414b, 415b are formed through the second flow cover hole 411b, 412b, 413b third flow cover hole 416b, 417b, 418b are formed through the third flow cover hole 416b, 417b, 418b are formed through the third flow cover hole 416b, 417b, 418b are formed through the third flow cover hole 419a, 419b are formed through the flow cover hole 410a, and communicate with one end and the other end of the air passage 409. Here, the first flow cover hole is Base plate The second and third flow cover holes are aligned with the inner end of the flow channel formed in 400, the other end of the flow channel is aligned with the outer end, and the fourth flow cover hole communicates with one end and the other end of the air flow channel. The second distance may be longer than the first distance and shorter than the third distance.

[0107] Referring to Figure 11, a first engaging projection 410b may be further formed on the outer circumference of the first through-hole 410a, projecting upward and downward.

[0108] Furthermore, molten projections 410c may be formed on the bottom surface of the flow cover 410 to engage along multiple flow channel edges of the base plate 400 (see Figure 12). When ultrasonic welding is performed after the flow cover 410 is installed on the upper surface of the base plate 400, the molten projections 410c melt and become integrated with the base plate 400. Through this, a tight engagement between the base plate 400 and the flow cover 410 is possible.

[0109] A pad 420 is placed on top of the flow cover 410. The pad 420 may be made of, for example, silicone, but is not limited to this material as long as it has a certain elastic force.

[0110] Multiple second engaging projections 410d are formed protruding from the upper surface of the flow cover 410, and a strong engagement between the flow cover 410 and the pad 420 is achieved by the second engaging projections 410d engaging with the engagement grooves 420c of the pad 420. In addition, a strong engagement between the two components can be achieved by inserting and coupling the first engaging projection 410b of the flow cover 410 into the second through hole 420a of the pad 420.

[0111] The pad 420 has a second through-hole 420a aligned with the first through-hole 410a, and a plurality of first pad holes 421a, 422a, 423a, 424a, 425a, 426a, 427a, and 428a are formed through the pad on a first circumference at a first distance from the second through-hole 420a, a plurality of second pad holes 421b, 422b, 423b, 424b, and 425b are formed through the pad on a second circumference at a second distance from the second through-hole 420a, a plurality of third pad holes 426b, 427b, and 428b are formed through the pad on a third circumference at a third distance from the second through-hole 420a, and fourth pad holes 429a and 429b are formed through the pad, communicating with one end and the other end of the air passage 409. Here, the first pad hole aligns with the first flow cover hole, the second pad hole aligns with the second flow cover hole, the third pad hole aligns with the third flow cover hole, and the fourth pad hole aligns with the fourth flow cover hole.

[0112] On the upper surface of the pad 420, a protruding portion is formed that extends from the area where multiple second pad holes 421b, 422b, 423b, 424b, 425b, multiple third pad holes 426b, 427b, 428b, and a fourth pad hole 429b communicating with the other end of the air passage are formed, and which narrows towards the top. The formation of this protruding portion solves the problem that the diameter of the pad holes decreases in a way that is different from what was intended, even when the pad 420 is in close contact with the outer chamber 100 and the base plate 400.

[0113] The amplification module 600 is engaged with the outer chamber 100 and configured to contain a pre-treated sample. Pre-treated sample means that the genome, such as DNA or RNA, contained in the sample has been eluted (lysis) into the reagent. When the genome extraction device 1000 according to the present invention is engaged with a diagnostic instrument (not shown), an amplification process (such as PCR) is performed on the genome contained in the amplification module 600.

[0114] Referring to Figures 1 and 2, the amplification module 600 engages with the outer chamber 100 in the vertical direction. In other words, the upper part 631 of the housing 630 of the amplification module 600 engages with the outer chamber 100 such that it is higher than the lower part 632 of the housing 630.

[0115] Referring to Figures 17-25, the amplification module 600 includes a body 610, inlets 621, 622, a housing 630, a gas transport passage 640, and an extractant transport passage 650.

[0116] The body 610 is the outer shape of the amplification module 600, and on one side of the body 610, inlets 621 and 622 are formed that engage with the discharge holes 128 and 129 of the outer chamber 100.

[0117] The inlet ports 621 and 622 engage with the discharge holes 128 and 129, and serve as inlets for the extract discharged from the discharge holes 128 and 129 to be introduced into the containment section 630.

[0118] An amplification module 600 according to an embodiment of the present invention may have two injection ports 621, 622, but is not particularly limited thereto, and embodiments having more than two injection ports may also be included in the scope of the present invention.

[0119] In the following description, we will specifically explain the amplification module 600 according to an embodiment of the present invention, assuming that it has two injection ports 621 and 622.

[0120] One of the two inlets 621, 622, inlet 621, is connected to the air passage 409, and the other inlet 622 is connected to the liquid passage 408. That is, the extract containing the pre-treated sample flows in through the other inlet 622, and in the process, the air in the containment section 630 is discharged into the air passage 409 through one of the inlets 621.

[0121] On the other side of the body 610, a receiving section 630 is formed, which is a space for receiving the extract that flows in through the inlet 621.

[0122] In one example, the housing section 630 can be manufactured in a form that penetrates both sides of the body 610, but in another example, it can be manufactured in a form that penetrates only one side and not the other. Both embodiments are identical in that the open portion is sealed by a sealing member. Therefore, the extract and air are introduced into or discharged from the housing section 630 only through the gas transport passage 640 and the extract transport passage 650.

[0123] The housing section 630 according to the embodiment of the present invention may be provided in one or more units within a single amplification module 600. Figure 17 shows an amplification module with one housing section, Figure 20 shows an amplification module with two housing sections, and Figure 23 shows an amplification module with four housing sections.

[0124] The storage section 630 may have a substantially trapezoidal shape, and more specifically, it is preferable that it has a trapezoidal shape with rounded edges.

[0125] Here, "trapezoidal" refers to a shape in which the width narrows as it moves away from the gas transport passage 640 and the extract transport passage 650. The shape of the containment section 630 solves the problem of bubbles forming even when the extract is injected through the extract transport passage 650. If bubbles remain in the containment section 630, a detection failure may occur during the fluorescence detection process after the amplification process; therefore, this problem can be solved by changing the shape of the containment section 630.

[0126] The containment section 630 is equipped with primers and probes necessary for genome amplification. The amplification module 600 according to the embodiment of the present invention is equipped with one or more containment sections 630, and each containment section 630 can be equipped with different types of primers and probes. Therefore, it has the advantage of being able to perform multiple detection processes simultaneously on a genome extracted from a single sample. For example, one containment section 630a is equipped with primers and probes for coronavirus amplification, and another containment section 630b is equipped with primers and probes for influenza virus amplification, making it possible to perform various detection processes simultaneously with a single amplification module 600.

[0127] The gas transport passage 640 is formed on one surface 611 of the body 610 and is configured to connect the inlet 621 and the upper part 631 of the housing section 630. Conversely, the extractant transport passage 650 is formed on the opposite surface 612, opposite to the aforementioned surface 611, and is configured to connect the inlet 622 and the lower part 632 of the housing section 630.

[0128] The gas transport passage 640 serves as a passage for the gas to move within the containment section 630. The flow path of the amplification module 600 is for the genome extraction device. 1000 It communicates with and simultaneously has the characteristics of a closed flow path. Since the containment section 630 is filled with air before the extract is injected, when the extract is injected, an appropriate volume of air must be discharged to the outside. In this invention, the air inside the containment section 630 is discharged through the gas transfer passage 640 to the air passage 409 via the inlet 621, thereby reducing the pressure inside the containment section 630 and solving the problem of bubbles generated by residual air. Similar to the containment section 630, the gas transfer passage 640 also has curved connecting sections without any angular parts to minimize the generation of bubbles.

[0129] Since gas is lighter than liquids such as extractants, the gas transport passage 640 is connected to the end of the upper part 631 of the containment section 630.

[0130] If multiple storage compartments 630 are provided, it is preferable that the lengths of the gas transfer passages 640 connected to each storage compartment 630 are different.

[0131] In embodiments where multiple containment sections 630 are provided, the extract is injected from the lower containment section, and the higher the containment section, the more delayed the injection of the extract. Therefore, the time it takes for air to be discharged from a containment section 630 also differs depending on its position. In other words, the lower the containment section, the faster the air is discharged through the gas transfer passage 640.

[0132] Furthermore, not only the air in the containment section 630 but also the extractant introduced into the containment section 630 can be discharged through the gas transfer passage 640. Since multiple gas transfer passages 640 are connected to each other, extractant discharged through one gas transfer passage 640 may be introduced into other containment sections along the other gas transfer passages 640, potentially leading to a problem of mixing of extractant or amplification products. To solve this problem, the present invention makes the length of the gas transfer passage 640 longer the further down the containment section 630 it is connected to, thereby solving the problem of mixing of extractant or amplification products.

[0133] The gas transfer passages 640 can be configured to have different lengths, as shown in Figure 21 or as shown in Figure 24.

[0134] Referring to Figure 24, the gas transport passage 640 is formed on one surface 611 of the body 610 and communicates with the gas discharge passage 633 which is connected to the upper part 631 of the containment section 630. It includes a communication hole 641 that penetrates the body 610, a transport passage 642, a storage passage 643, and a passage pattern forming section 644.

[0135] The passage pattern forming section 644 is configured to form a predetermined passage pattern in the moving passage 642. Taking Figure 24 as an example, the passage pattern forming section 644 can have a semicircular shape, and by combining the semicircular passage pattern forming section 644 with the linear moving passage 642, a passage pattern as shown in Figure 24 may be manufactured. The passage pattern forming section 644 can form the passage pattern shown in Figure 24 by alternately combining it with the moving passage 642 on the left and right sides of the linear moving passage 642. Here, "combination" means that the empty space in the moving passage 642 is filled with the shape of the passage pattern forming section 644, and the filled space cannot be passed through by fluid.

[0136] In other words, the portion of the gas transport passage 640 that is combined with the passage pattern forming section 644 corresponds to the transport passage 642, and the portion of the gas transport passage 640 that is not combined corresponds to the storage passage 643.

[0137] The length of the gas transfer passage 640 increases proportionally to the number of passage pattern forming sections 644 and storage passages 643, and the lower the containment section, the more passage pattern forming sections 644 and storage passages 643 it has. Through this, mixing of the extract or amplification product contained in the containment section 630 can be prevented.

[0138] The extractant transport passage 650 is formed on one side 611 of the body 610 and the opposite side 612, and is configured to connect the inlet 622 and the lower part 632 of the housing section 630. The extractant transport passage 650 serves as a passage through which the extractant pretreated by the genome extraction device 1000 moves.

[0139] In order to prevent mixing of the extract or amplification product contained in the containment section 630, and to ensure that the same amount of extract is introduced into each containment section 630, if there are multiple containment sections 630, the length of each extract transfer passage 650 may be the same, or if the lengths differ, the thickness of each extract transfer passage 650 may differ.

[0140] Furthermore, in order to prevent the generation of bubbles as the extract moves through the extract transfer passage 650, the extract transfer passage 650 has no sharp edges and the connecting parts of the passage are curved to minimize the generation of bubbles.

[0141] Referring to Figure 25, the extractant transport passage 650 extends from the inlet 622 and branches at one point. From the branching point, the passage is thicker towards the lower containment section and thinner towards the upper containment section. Since the thinner the passage, the faster the extractant passes through, the same amount of extractant may be introduced into both the upper and lower containment sections.

[0142] The piston 700 is inserted into the piston insertion section 108 of the outer chamber 100 and is configured to draw in reagents contained in the outer chamber 100 or discharge reagents drawn into the outer chamber 100 or the amplification module 600 by moving up and down.

[0143] Referring to Figures 3 and 12, the piston 700 includes an upper piston 710 and a lower piston 720.

[0144] The upper piston 710 is open at the top, and a fluid reservoir 701 is formed inside to contain the inhaled fluid. A sealing portion 711 is installed inside the upper piston 710. The outer surface of the sealing portion 711 is in close contact with the inner surface of the upper piston 710, making it impossible for fluid to enter or exit through the space between the outer surface of the sealing portion 711 and the inner surface of the upper piston 710. A recessed drive unit mounting portion 711a is formed in the center of the sealing portion 711, into which the drive unit (not shown) of the diagnostic device engages. The drive unit (not shown) of the diagnostic device engages with the drive unit mounting portion 711a and raises and lowers the sealing portion 711 inside the upper piston 710, thereby drawing fluid into the fluid reservoir 701 or discharging the fluid contained in the fluid reservoir 701 to the outside.

[0145] An engagement structure that meshes with the lower piston 720 may be formed on the bottom surface of the upper piston 710, and a first hole 712 connected to the liquid port of the lower piston 720 and a second hole 713 connected to the filter port of the lower piston 720 are formed through it. The second hole 713 is formed to have a diameter smaller than the filter mounting space of the filter port in order to prevent the support structure and the filter from becoming detached.

[0146] The lower piston 720 is fixed by engaging with a coupling structure formed on the bottom surface of the upper piston 710.

[0147] The lower piston 720 may include a disc-shaped body 721, a shaft 722 formed to protrude outward from the center of the body 721, and liquid ports 723 and filter ports 724 located at the same distance from the center of the body 721.

[0148] The liquid port 723 is used to draw in, mix, and discharge samples and reagents into the piston 700, while the filter port 724 is used to wash the genome collection filter and to separate genomes from the genome collection filter.

[0149] Furthermore, a groove recessed toward the center may be formed on the outer circumference of the body 721 of the lower piston 720. This groove serves to remove the vacuum that may be generated inside the extraction device during liquid movement.

[0150] The liquid port 723 and the filter port 724 are positioned on the same circumference, separated from each other at a certain angle. For example, the two ports, the filter port 724 and the liquid port 723, may be positioned 18 to 36 degrees apart from each other, or more specifically, they may be positioned at a distance of 22.5 degrees from each other. When using a stepper motor that rotates in 16 steps, the positions of the liquid port 723 and the filter port 724 can be changed with a single drive.

[0151] The filter port 724 of the lower piston 720 may include a filter mounting space 725, in which a filter and a support structure may be placed. The filter for genome collection may be a glass fiber filter having various particle sizes, and the support structure serves to fix the filter for genome collection.

[0152] The support structure may be made of a porous plastic material with a certain particle size to prevent the filter from detaching during fluid discharge and to maintain a constant pressure.

[0153] The drive unit 800 is connected to the drive unit (not shown) of the diagnostic equipment and acts as a mediator, rotating the piston 700 at a constant angle.

[0154] The drive unit 800 may include an engagement groove formed in the center of one surface to engage with the shaft 722, and a drive groove formed on the other surface to engage with the drive unit (not shown) of a diagnostic device.

[0155] The drive unit 800 engages with the piston 700 to position the liquid port 723 and the filter port 724 at the appropriate location of the first discharge port of the outer chamber 100, so that the various chemical reactions required in the genome extraction stage can be carried out inside a single device.

[0156] The liquid port 723 and the filter port 724 are spaced at a certain angle apart, and the drive unit 800 rotates the ports to positions suitable for each stage during genome extraction.

[0157] The bead chamber 900 includes a first bead chamber 910, a second bead chamber 920, and a dehumidifying chamber 930, which are the first bead chamber partition 901 and the second bead chamber bulkhead The chambers are partitioned by 902. The first bead chamber 910 is inserted into the first space 106 of the outer chamber 100, and the second bead chamber 920 is inserted into the first space 107 of the outer chamber 100.

[0158] Similar to the inner chamber 200, the upper opening of the bead chamber 900 is also sealed by a third sealing member S3, which is perforated by third protruding members 316, 317 formed on the bottom surface of the cover 300 when the cover 300 engages with the outer chamber 100. The opening of the upper opening of the bead chamber 900 by the third protruding members 316, 317 allows a corresponding amount of air to be discharged through the perforated portion even if fluid is subsequently introduced into the first bead chamber 910 and the second bead chamber 920.

[0159] The lower opening of the bead chamber 900 is provided in an open form without being sealed by a separate sealing member. Dry beads, more specifically freeze-dried beads, are stored in the bead chamber 900, but dry beads are susceptible to moisture. In the genome extraction apparatus according to the present invention, the lower opening of the bead chamber 900, the first space of the outer chamber 100, the flow cover 410, the pad 420, the flow path of the base plate 400, and the flow path of the amplification module 600 are in communication with each other, but form a closed flow path that is not exposed to the outside air, thereby minimizing the inflow of moisture into the bead chamber 900.

[0160] The first bead chamber 910 may store several dried beads (b1) necessary for genome extraction, and the second bead chamber 920 may store several dried beads (b2) necessary for genome amplification.

[0161] A first bead holder 911 is installed at the upper opening of the first bead chamber 910, configured to keep the dried bead (b1) inside without being discharged to the outside, and a first dehumidification unit 912 is installed in the dehumidification chamber 930 for dehumidifying the internal space of the first bead chamber 910. Here, the dried bead necessary for genome amplification is provided in the form of a capsule, for example, but is not limited to this.

[0162] A second bead holder 921 is installed at the upper opening of the second bead chamber 920, configured to keep the dry bead (b2) inside without being discharged to the outside. A second dehumidifying unit 922 is installed above the second bead holder 921 to dehumidify the inside of the second bead chamber 920. The third sealing member S3 seals the second bead chamber 920 so that it does not communicate with the dehumidifying chamber 930 and the first bead chamber 910, but seals the first bead chamber 910 and the dehumidifying chamber 930 so that they communicate with each other. This will be explained in detail with reference to Figures 26 and 27.

[0163] The aforementioned effect is achieved through the configuration of a height difference between the first bead chamber partition 901 and the second bead chamber partition 902. Referring to Figures 26 and 27, the second bead chamber partition 902, which separates the second bead chamber 920 and the dehumidification chamber 930, has a higher height than the first bead chamber partition 901, which separates the first bead chamber 910 and the dehumidification chamber 930.

[0164] In other words, the upper part of the second bead chamber partition 902 extends to the same height as the upper part of the outer partition forming the second bead chamber 920, while the upper part of the first bead chamber partition 901 extends to a height lower than the upper part of the outer partition forming the first bead chamber 910.

[0165] Therefore, even if the upper opening of the bead chamber 900 is sealed by the third sealing member S3, the first bead chamber 910 and the dehumidification chamber 930 can communicate with each other through the space between the first bead chamber partition wall 901 and the third sealing member S3. Consequently, the first bead chamber 910 is dehumidified by the second dehumidification unit 912 installed inside the dehumidification chamber 930.

[0166] Lower opening of the first bead chamber 910 914 (That is, the outlet of the first bead chamber) and the lower opening of the second bead chamber 920 924(That is, the discharge port of the second bead chamber) is a discharge passage that narrows as it moves from the bead chamber 900 towards the base plate 400. 913,923 It is formed at the end of the structure.

[0167] discharge passage 913,923 A dry bead may be contained inside the discharge passage. 913,923 A bead holder is installed at the top, and a discharge passage 913,923 This prevents the bead contained within from leaking out.

[0168] discharge passage 913,923 It can have a so-called tapered shape, which narrows towards the base plate 400. And the discharge passage 913,923 Lower opening located at the end 914,924 The diameter is smaller than the diameter of the dry bead, and the lower opening 914,924 The bead is not discharged to the outside through this opening. 914,924 Through the discharge passage 913,923 The fluid flows into the interior, melts the dry bead, and only through the form of the fluid does it open at the bottom. 914,924 The fluid may be discharged to the outside (to the piston's fluid reservoir or amplification module) through this.

[0169] Here, the discharge passage of the first bead chamber 910 where the dried beads necessary for genome amplification are stored. 913 This is the discharge passage of the second bead chamber 920. 923 It has a wider diameter and can narrow towards the base plate 400.

[0170] The first bead chamber 910 is configured to receive the final fluid before the pre-treated extract is fed into the amplification module 600. Accurate detection results can only be obtained if the fluid fed into the first bead chamber 910 is fed into the housing section 630 of the amplification module 600 without maximizing residual fluid within the chamber. Therefore, in this invention, the discharge passage of the first bead chamber 910... 913 This is the discharge passage of the second bead chamber 920923 By having a wider diameter and shaping it to become narrower, the amount of fluid remaining in the first bead chamber 910 was minimized.

[0171] Furthermore, the bead chamber 900 according to the present invention has first locking projections 903 and 904 extending from the bottom surface of the outer partition wall of the first bead chamber 910 and the second bead chamber 920. As shown in Figures 28 and 30, the first locking projections 903 and 904 may be formed to extend toward the base plate 400 and protrude outward.

[0172] The outer chamber 100, which engages with the bead chamber 900, has a second locking projection on one side of the outer chamber partition wall that divides a plurality of first spaces. 119 When a force is applied to the bead chamber 900 toward the base plate 400, the first locking projections 903 and 904 move toward the second locking projection 119 Through this process, the two components engage with each other, resulting in a strong engagement between them. The first locking projections 903 and 904 engage with the second locking projection 119 When engaged, the relative position of the bead chamber 900 with respect to the outer chamber 100 is fixed.

[0173] The extraction method according to the embodiment of the present invention will be described in detail below.

[0174] First, (a) the inner chamber engages with the outer chamber through the upper openings of a plurality of first spaces in the outer chamber. Here, it is preferable that the fixing portion of the inner chamber engages with the outer chamber while engaged with the inner chamber engaging portion of the safety clip.

[0175] Next, (b) the cover engages with the outer chamber, and (c) the safety clip is removed from the outer chamber.

[0176] Next, (d) the cover is pressurized, and the first sealing member that seals the upper opening of the inner chamber is broken by the first protruding member formed on the bottom surface of the cover, and the second sealing member that seals the lower opening of the inner chamber is broken by the second protruding members formed on the bottom surfaces of the plurality of first spaces of the outer chamber, and the reagent contained in the inner chamber flows out into the plurality of first spaces, and (e) the drive unit drives the reagent that has flowed out into the plurality of first spaces, and after being drawn into the fluid containment part inside the upper piston and mixed, the mixed reagent is discharged to the amplification module.

[0177] The aforementioned step (e) is carried out in multiple stages. Step (e) will be described in more detail below. First, the sample to be analyzed is introduced into one of the multiple first spaces of the outer chamber through the sample input hole of the cover (e1).

[0178] Next, (e2) the piston installed in the piston housing of the outer chamber rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of one of the first spaces into which the sample to be analyzed is introduced.

[0179] Next, (e3) the sealing portion installed in the internal space of the piston rises, and the analyte sample contained in one of the first spaces is drawn into the fluid containment portion inside the outer chamber.

[0180] Next, (e4) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom of the other first space.

[0181] Next, (e5) the contact portion rises, and the first reagent contained in the other first space is drawn into the fluid containment portion inside the outer chamber, thereby mixing the analyte and the first reagent in the fluid containment portion.

[0182] Next, (e6) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of another first space.

[0183] Next, (e7) the contact portion rises, and the second reagent contained in the other first space is drawn into the fluid containment portion inside the outer chamber, thereby mixing the analyte with the first and second reagents.

[0184] Next, (e8) the piston rotates, and the filter port of the piston communicates with the first discharge hole formed in the bottom surface of the other first space.

[0185] Next, (e9) the sealing portion descends, and the mixed liquid contained in the fluid containment portion passes through the genome collection filter installed in the filter port and is discharged into the other first space.

[0186] Next, (e10) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom of the first space containing the first reagent, the second reagent, and other reagents.

[0187] Next, (e11) the sealing portion rises, and other reagents are drawn into and mixed in the fluid containment portion.

[0188] Next, (e12) the piston rotates, and the filter port of the piston communicates with the first discharge hole formed at the bottom of the first space containing the other reagents.

[0189] Next, (e13) the sealing section descends, and the mixed solution contained in the fluid containment section passes through the genome collection filter and is discharged into the first space containing the other reagents.

[0190] Next, (e14) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom of the first space containing the eluent.

[0191] Next, (e15) the contact area rises, and the eluent is drawn into the fluid containment section.

[0192] Next, (e16) the piston rotates, and the filter port of the piston communicates with the second discharge port formed at the bottom of the first space containing the beads necessary for genome amplification.

[0193] Next, (e17) the contact section descends, and the eluent contained in the fluid containment section passes through the genome collection filter and is discharged into the first space containing the beads necessary for genome amplification. In this step, the genome collected in the genome collection filter is separated from the genome collection filter and discharged together into the first space.

[0194] Next, the (e18) piston rotates, and the liquid port of the piston communicates with the second discharge port formed at the bottom of the first space containing the genome.

[0195] Next, (e19) the contact section rises, and the extract containing the genome is drawn into the fluid containment section.

[0196] Next, the (e20) piston rotates, and the piston's liquid port communicates with the amplification module.

[0197] Next, (e21) the contact section descends, and the extract containing the genome contained in the fluid containment section is discharged to the amplification module.

[0198] Next, (e22) the extract is introduced into the housing of the amplification module through the extract transport passage of the amplification module.

[0199] Next, (e23) the remaining air in the containment section is discharged to the outside of the amplification module through the gas transport passage of the amplification module.

[0200] Next, (e24) the amplification device applies heat above a predetermined temperature to the housing section, and the genome is amplified.

[0201] Next, (e25) the presence or absence of disease infection in the sample to be analyzed is determined based on the fluorescence intensity of the amplified genome product.

[0202] Although the embodiments shown in the drawings have been described in this specification with reference to those skilled in the art so that the present invention can be easily understood and reproduced, these are merely illustrative examples, and those skilled in the art will understand that a variety of modifications and equivalent other embodiments are possible from the embodiments of the present invention. Therefore, the scope of protection of the present invention must be defined by the claims. [Explanation of symbols]

[0203] S1: First sealing member S2: Second sealing member S3: Third sealing member S4, S5: Sealing components 100: Outer chamber 100a: Upper outer surface 100b: Lower outer surface 101,102,103,104,105,106,107: 1st space 108: Piston insertion section 109: Safe Placement Department 111,112,113,114,115: Second protruding member 111a, 112a, 113a, 114a, 115a: Protrusion 111b, 112b, 113b, 114b, 115b: Wing section 119:Second locking protrusion 121,122,123,124,125: 1st discharge hole 126,127,129:Second discharge hole 128: Air vent 130: Insertion space 131: Stopper 200: Inner Chamber 201,202,203,204,205:Second space 210: Upper inner chamber 220: Lower inner chamber 230: Fixed part 300: Cover 301: Cover body 302: Lid 307: First insertion hole 308: Second insertion hole 311, 312, 313, 314, 315: First protruding member 316,317: Third protruding member 320: Separation member 400: Base plate 400a: Piston drive unit insertion hole 401, 402, 403, 404, 405, 406, 407, 408: Liquid flow path 409: Airflow channel 410: Flow Cover 410a: 1st through hole 410b: 1st engagement protrusion 410c: Melting protrusion 410d: Second engagement protrusion 411a, 412a, 413a, 414a, 415a, 416a, 417a, 418a: First flow cover hole 411b, 412b, 413b, 414b, 415b: Second flow cover hole 416b, 417b, 418b: Third flow cover hole 419a, 419b: Fourth flow cover hole 420: Pad 420a: 2nd through hole 421a, 422a, 423a, 424a, 425a, 426a, 427a, 428a: First pad hole 421b, 422b, 423b, 424b, 425b: Second pad hole 426b, 427b, 428b: Third pad hole 429a, 429b: Fourth pad hole 420c: Engagement groove 500: Safety clip 510: Outer chamber engagement part 520: Handle 530: Upper extension 540: Side extension 541: Cover support member 542: Inner chamber engagement part 600: Amplifier Module 610: Body 611: One side 612: Opposite side 621,622: Inlet 630: Containment Unit 631: Top 632: Lower part 633: Gas discharge passage 640: Gas transport passage 641:Communication hole 642: Access Passage 643: Storage passage 644: Passage pattern forming section 650:Extract liquid transfer passage 700: Piston 701: Fluid containment section 710: Upper Snapshot 711: Close-up section 711a: Drive unit installation section 712: Hall 1 713: Second Hall 720: Lower piston 721: Torso 722: Shaft 723: Liquid port 724: Filter port 800: Drive unit 900: Bead Chamber 901: First bead chamber bulkhead 902: Second bead chamber bulkhead 903,904: 1st locking protrusion 910: First bead chamber 911: First bead holder 912: 1st dehumidification section 913: Discharge passage 914: Lower opening 920: Second bead chamber 921: Second bead holder 922:Second dehumidification section 923: Discharge passage 924: Lower opening 930: Dehumidifying Chamber 1000: Genome extraction device

Claims

1. A chamber in which the interior is divided into multiple first spaces by a chamber partition, a sample is contained in one of the multiple first spaces, different reagents are contained in the other first spaces, and an outlet hole is formed at the bottom; A base plate that engages with the lower part of the chamber, wherein a plurality of flow channels communicating with the plurality of first spaces are formed on its upper surface; A flow cover disposed between the chamber and the base plate, with flow cover holes formed through it that connect the plurality of first spaces and the plurality of flow paths; and A pad made of an elastic material, disposed on the upper part of the flow cover, with pad holes formed through it that connect the flow cover hole and the plurality of first spaces; The plurality of flow channels include a flow channel that extends radially outward from a portion a predetermined distance from the center of the base plate, with one end positioned on a first circumference at a first distance from the center of the base plate and the other end positioned on a second circumference at a second distance from the center of the base plate. The flow cover hole includes a first flow cover hole located on the first circumference and a second flow cover hole located on the second circumference. The pad hole includes a first pad hole located on the first circumference and a second pad hole located on the second circumference. One end of the plurality of flow paths, the first flow cover hole, and the first pad hole are aligned, The other end of some of the flow channels in the plurality of flow channels is aligned with the second flow cover hole and the second pad hole, The pad has a protrusion that narrows as it extends upward from the portion where the second pad hole is formed. Genome extraction device.

2. The aforementioned flow cover is made of plastic material. The genome extraction apparatus according to claim 1, wherein the pad is made of a silicone material.

3. The genome extraction apparatus according to claim 2, wherein the plurality of channels further include channels whose other ends are located on a third circumference at a third distance greater than a second distance from the center of the base plate.

4. The aforementioned plurality of channels are The genome extraction apparatus according to claim 3, further comprising an air channel, one end of which is located at a distance from the center of the base plate that is longer than the first distance and shorter than the second distance, and the other end of which is located at a distance from the center of the base plate that is longer than the second distance and shorter than the third distance.

5. The aforementioned flow cover has a first through-hole formed in which the drive unit is installed. First engaging protrusions are formed on the outer circumference of the first through hole, projecting upwards and downwards, respectively. The genome extraction apparatus according to claim 4, wherein the first engaging projection is inserted into the drive unit insertion hole of the base plate and the second through hole of the pad.

6. The genome extraction apparatus according to claim 5, wherein the flow cover has a plurality of first flow cover holes formed through it on a first circumference at a first distance from the first through hole, a plurality of second flow cover holes formed through it on a second circumference at a second distance from the first through hole, a plurality of third flow cover holes formed through it on a third circumference at a third distance from the first through hole, and a fourth flow cover hole formed through it that communicates with one end and the other end of the air passage.

7. The bottom surface of the aforementioned flow cover is The genome extraction apparatus according to claim 6, wherein molten projections are formed to engage along the edges of the plurality of flow channels.

8. The genome extraction apparatus according to claim 7, wherein the pad has a plurality of first pad holes formed through it on a first circumference at a first distance from the second through hole, a plurality of second pad holes formed through it on a second circumference at a second distance from the second through hole, a plurality of third pad holes formed through it on a third circumference at a third distance from the second through hole, and a fourth pad hole formed through it that communicates with one end and the other end of the air passage.

9. The genome extraction apparatus according to claim 8, wherein the diameter of the third pad hole is larger than the diameters of the first pad hole and the second pad hole.

10. The genome extraction apparatus according to claim 8, comprising a portion that protrudes from the upper surface of the pad while being connected to the upper part of the third pad hole, and which widens towards the flow cover.

11. The genome extraction apparatus according to claim 1, wherein the plurality of channels are asymmetrical on a cross-section including the center of the base plate.

12. The genome extraction apparatus according to claim 2, wherein the flow cover is fused and fixed onto the base plate.

13. The other end of one of the other flow paths in the plurality of flow paths is aligned with the plurality of third flow cover holes and the plurality of third pad holes. The genome extraction apparatus according to claim 8, wherein the air channel, the fourth flow cover hole, and the fourth pad hole are aligned.

14. One end of the plurality of flow paths communicates with a fluid storage section inside a piston installed in the chamber. The genome extraction apparatus according to claim 13, wherein the other end of the plurality of channels communicates with the discharge holes of the plurality of first spaces of the chamber or with an amplification module that contains the genome to be amplified.

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