Centrifugal tube

Abstract

To provide a centrifugal separation tube that enables PRP to be easily collected, in which a capacity of the centrifugal separation tube is made variable with a simple configuration, and abrasive wear is hard to occur in a sealant used in that case.SOLUTION: A centrifugal separation tube 1 includes a tube body 10, and a bottomed cylindrical slide adapter 50 to which a lower part of the tube body 10 is inserted. The centrifugal separation tube 1 includes a screw-fitting portion of the tube body 10 and the slide adapter 50, and includes slide-contact regions 16, 52 with which the tube body 10 and the slider adapter 50 are brought into slide contact with a lower part of the screw-fitting portion. An outer diameter D1 of the tube body 10 in the screw-fitting portion is larger than an outer diameter D2 of the tube body of the slide-contact region 16, and a packing 60 is provided in the slide-contact region 16.SELECTED DRAWING: Figure 8B

Description

Technical Field

[0001] The present invention relates to a centrifugal separation tube suitable for separating PRP. Background Art

[0002] PRP (Platelet-Rich Plasma) is a platelet-rich plasma layer obtained by centrifuging blood. PRP contains various growth factors and can be used to repair damaged sites in biological tissues. Therefore, therapy using PRP has been adopted in orthopedics, plastic surgery, dentistry, etc. While further popularization is expected, it is also desired to study and elucidate its mechanism.

[0003] However, conventional safety-approved PRP collection kits are expensive, and the PRP collection operation is also complicated. Therefore, PRP cannot be easily used in both clinical medical practice and research.

[0004] As a centrifugal separation tube that allows easy collection of PRP from blood using a general-purpose centrifuge and suppresses the intrusion of foreign substances such as viruses and bacteria floating in the air into the tube, a centrifugal separation tube in which the inner diameter of the middle part of the tube body is smaller than that of the upper and lower parts has been proposed (Patent Document 1). In this centrifugal separation tube, the position and volume of the middle part in the centrifugal separation tube are adjusted such that the buffy coat layer containing PRP is located in the small-diameter middle part after centrifugation of blood. Further, a cap formed of a flexible resin is fitted to the upper end of the tube body, and blood injection and PRP collection are performed by penetrating the cap with an injection needle.

[0005] As described in Patent Document 1, for a centrifuge tube having a narrow-diameter intermediate section, it has also been proposed to provide a movable lower gasket inside the large-diameter lower section of the centrifuge tube, and to push the lower gasket with a pusher to make the volume of this large-diameter section variable, so that the buffy coat layer can be moved to the narrow-diameter section after centrifugation (Patent Document 2). In this centrifuge tube, blood is injected or PRP is collected by passing a needle through the upper gasket made of soft resin provided on the upper cap. [Prior art documents] [Patent Documents]

[0006] [Patent Document 1] Special Publication No. 2013-514874 [Patent Document 2] US9775942B2 [Overview of the project] [Problems that the invention aims to solve]

[0007] As described in Patent Document 2, if the volume of the lower part of the centrifugal separator is made variable, the buffy coat layer can be moved to the narrow-diameter section in the middle of the centrifugal separator, making it easier to collect the buffy coat layer. However, in the centrifugal separator described in Patent Document 2, the lower gasket that moves inside the large-diameter section at the bottom of the centrifugal separator is formed by a connector that screws onto the inner circumferential surface of the large-diameter section and a sealing member fitted to the connector. As a result, the sealing member may wear down or be damaged due to the movement of the lower gasket. Therefore, there is a concern that liquid leakage may occur from the centrifugal separator.

[0008] Furthermore, in the centrifugal separator described in Patent Document 2, a pusher is required that fits into the lower gasket, separate from the lower gasket that moves inside the large-diameter lower portion of the centrifugal separator. As a result, the structure around the lower gasket of the centrifugal separator becomes complex, and the manufacturing cost of the centrifugal separator increases.

[0009] In contrast to such conventional technologies, the present invention aims to provide a centrifuge tube that enables easy collection of PRP by centrifugation of blood, by making the volume of the centrifuge tube easily adjustable and reducing wear and damage to the sealing material used in that case. [Means for solving the problem]

[0010] The inventors have discovered that, in order to make the volume of the lower part of the centrifugal separator variable, a bottomed cylindrical member (hereinafter referred to as a slide adapter) is provided that is screwed onto the lower part of the centrifugal separator to allow the volume of the centrifugal separator to be expanded or contracted. By forming the sliding contact region that maintains sealing performance with the centrifugal separator and the screwed portion with the centrifugal separator separately, and by providing a packing in the sliding contact region, the volume of the lower part of the centrifugal separator can be made variable with a simple configuration, and wear and damage to the packing can also be prevented, thus completing the present invention.

[0011] That is, the present invention relates to a centrifugal separator having a tube body and a bottomed cylindrical slide adapter into which the lower part of the tube body is inserted, The tube body and the slide adapter have a threaded portion, and below the threaded portion there is a sliding contact area where they slide against each other. The outer diameter of the tube body in the threaded portion is larger than the outer diameter of the tube body in the sliding contact area. The present invention provides a centrifugal separator tube having a packing in the sliding contact area. [Effects of the Invention]

[0012] According to the present invention, a bottomed cylindrical slide adapter is screwed onto the lower part of the centrifuge tube body, so the volume at the bottom of the centrifuge tube can be adjusted with a simple configuration without the need for a separate pusher.

[0013] Furthermore, when the slide adapter is screwed to the lower part of the tube body of the centrifugal separation tube, and the slide adapter is moved in the axial direction of the tube body, a region where the tube body and the slide adapter are in sliding contact is formed separately from the screwed portion between the tube body and the slide adapter, and a packing is provided therein, so the risk of wear and damage to the packing can be reduced. [BRIEF DESCRIPTION OF THE DRAWINGS]

[0014] [Figure 1A] Figure 1A is a perspective view of the centrifugal separation tube according to the example, in a state where an injection port and a sampling port are each covered with a plug. [Figure 1B] Figure 1B is a cross-sectional view of the centrifugal separation tube according to the example, in a state where an injection port and a sampling port are each covered with a plug. [Figure 2A] Figure 2A is a perspective view of the top surface side of a cap with an open plug. [Figure 2B] Figure 2B is a perspective view of the back side of a cap with an open plug. [Figure 3A] Figure 3A is a top view of a cap with an open plug. [Figure 3B] Figure 3B is a cross-sectional view of the cap with an open plug taken along line K-K. [Figure 3C] Figure 3C is a cross-sectional view of the cap with an open plug taken along line N-N. [Figure 4] Figure 4 is a cross-sectional view of a cap with a closed plug. [Figure 5A] Figure 5A is a side view of the tube body. [Figure 5B] Figure 5B is a cross-sectional view of the tube body. [Figure 5C] Figure 5C is a cross-sectional view of the tube body showing the sliding contact region of the tube body. [Figure 6A] Figure 6A is a perspective view of the packing. [Figure 6B] Figure 6B is a cross-sectional view of the packing. [Figure 7A] Figure 7A is a perspective view of the slide adapter. [Figure 7B]FIG. 7B is a cross-sectional view of a slide adapter. [Figure 8A] FIG. 8A is a cross-sectional view illustrating a method of using a centrifuge tube. [Figure 8B] FIG. 8B is a cross-sectional view illustrating a method of using a centrifuge tube. [Figure 8C] FIG. 8C is a cross-sectional view illustrating a method of using a centrifuge tube. [Figure 8D] FIG. 8D is a cross-sectional view illustrating a method of using a centrifuge tube. [Figure 8E] FIG. 8E is a cross-sectional view illustrating a method of using a centrifuge tube. [Figure 8F] FIG. 8F is a cross-sectional view illustrating a method of using a centrifuge tube. [Figure 8G] FIG. 8G is a cross-sectional view illustrating a method of using a centrifuge tube. MODE FOR CARRYING OUT THE INVENTION

[0015] Hereinafter, the present invention will be described in detail with reference to the drawings. In each drawing, the same reference numeral represents the same or equivalent constituent element.

[0016] (Overall Structure) FIG. 1A is a perspective view of a centrifuge tube 1 according to an embodiment of the present invention, and FIG. 1B is a cross-sectional view thereof. This centrifuge tube 1 is usable in a general centrifugal separator, and includes a tube body 10, a cap 20 covering an upper end opening of the tube body 10, and a bottomed cylindrical slide adapter 50 screwed to a lower portion 12 of the tube body 10. Further, below the threaded engagement portion between the tube body 10 and the slide adapter 50 (that is, the portion actually engaged by threads), there is a sliding contact region where the tube body can be in sliding contact with the slide adapter, and a packing 60 is fitted into a portion near the lower end of the sliding contact region.

[0017] The slide adapter 50 can move axially with respect to the tube body 1, thereby changing the axial position of a predetermined component after centrifugation. The packing 60 maintains liquid-tightness between the tube body 10 and the slide adapter 50 even while the slide adapter 50 is being moved.

[0018] The cap 20 has a cap body 21 that covers the upper end opening of the tube body 10. The cap body 21 has an injection port 30 for injecting a sample into the tube body 10 and a collection port 40 for collecting a predetermined component with a needle after centrifugation of the sample. The cap 20 also has an injection port stopper 31 that covers the injection port 30 and a collection port stopper 41 that covers the collection port 40. The injection port stopper 31 and the collection port stopper 41 are connected to the cap body 21 by strip-shaped connecting parts 32 and 42, respectively. Figures 1A and 1B show the state in which the injection port stopper 31 is fitted into the injection port 30 and the collection port stopper 41 is fitted into the collection port 40.

[0019] In this embodiment, the centrifuge tube 1 is suitably used for collecting PRP by centrifugation of blood, but the sample to be centrifuged in the present invention is not limited to this.

[0020] (Tube body) The tube body 10 is made of a transparent resin such as polycarbonate. It is preferable that it has passed biological safety tests. The tube body 10 has a funnel-shaped upper part 11, a cylindrical lower part 12, and an intermediate part 13 with a narrower inner diameter than the upper part 11 and lower part 12. A bottomed cylindrical slide adapter 50 is screwed onto the cylindrical lower part 12.

[0021] The combined container capacity of the centrifuge tube 1, comprising the upper section 11, middle section 13, lower section 12, and slide adapter 50, can be appropriately set according to the sample to be centrifuged. For applications involving the collection of PRP from blood, a capacity of approximately 15 mL is preferred. The volumes of the upper section 11, middle section 13, and lower section 12, as well as the diameter of the middle section 13, are appropriately set so that the buffy coat layer is located in the middle section 13 after centrifugation, facilitating the collection of PRP.

[0022] The upper part 11 is funnel-shaped. As shown in Figure 8A, when the injection needle N is brought into contact with this slope 11a, or the continuous portion between the upper part 11 and the middle part 12, blood S can be injected into the tube body 10 without blood pooling. It is preferable to mark the upper part 11 with lines for women 18a and men 18b, respectively, to indicate the amount of blood to be filled (Figure 1A). Although the proportion of red blood cells differs between men and women, by filling with female blood and male blood according to these lines 18a and 18b, it becomes easy to position the buffy coat layer in the middle part 13 after centrifugation, regardless of whether the blood is male or female.

[0023] Fins 14 are formed radially around the intermediate section 13. The fins 14 are provided to supplement the mechanical strength of the narrow-diameter intermediate section 13, and to stabilize the installation of the centrifugal separator 1 in a centrifugal separator that is compatible with a general centrifugal separator tube in which the intermediate section 13 is not formed to be narrow in diameter. In this embodiment, these fins 14 are formed not only on the intermediate section 13, but also from the portion of the upper section 11 closer to the intermediate section 13 to the portion of the lower section 12 closer to the intermediate section 13. This makes it possible to obtain sufficient mechanical strength even at the boundary between the intermediate section 13 and the lower section 12 where the diameter changes abruptly.

[0024] As shown in Figures 5A and 5B, the lower part 12 of the tube body 10 is generally cylindrical, but a protruding portion with a slightly larger outer diameter is formed in the center of its outer surface in the vertical direction, and a threaded portion 15 that screws into the slide adapter 50 is formed in this protruding portion. The dashed line in Figure 5C shows the position of the slide adapter when it has moved as far upward as possible with the threaded portion of the slide adapter screwed into the threaded portion 15 of the tube body 10, and the dashed line in the same figure shows the position of the slide adapter when it has moved as far downward as possible with the slide adapter 50 in the same screwed state. As can be seen from Figure 5C, the threaded portion 15 of the tube body 10 is shorter than the threaded portion 51 of the slide adapter (Figure 7B) in terms of the axial length of the tube body. The tube body 10 also has a sliding contact region 16 below the threaded portion 15 that slides against the slide adapter 50. The outer diameter D1 of the threaded portion 15 is larger than the outer diameter D2 of the sliding contact region 16 (Figure 5B). On the other hand, as will be described later, the inner diameter D3 of the threaded portion 51 of the slide adapter 50 that screws onto the threaded portion 15 of the tube body 10 is larger than the inner diameter D4 of the sliding contact area 52 below it (Figure 7B). Also, a groove 17 is formed on the outer surface of the portion of the sliding contact area 16 of the lower part 12 of the tube body 10 that is below the lower end, and a packing 60 is fitted into the groove 17 (Figures 5A and 5B). Therefore, when the threaded portion 51 of the slide adapter 50 is screwed onto the threaded portion 15 of the tube body 10, the sliding contact area 16 is less likely to be rubbed by the threaded portion 51 of the slide adapter 50, and wear and damage to the packing 60 due to rubbing against the threaded portion 51 of the slide adapter 50 can be suppressed.

[0025] The packing 60 is a sealing material suitable for movable parts. As the packing, a ring-shaped material made of a resin such as silicone can be used. In particular, from the viewpoint of improving sealing performance, a packing 60 as shown in Figures 6A and 6B is preferred, which has two connected rounded ring-shaped protrusions 61 on the outer surface and two rounded ring-shaped protrusions 62 on the inner surface.

[0026] (cap) In the present invention, a cap is provided as needed, but it is preferable to provide a cap in order to keep the centrifugal tube clean. The cap 20 of the centrifugal tube 1 in this embodiment has a cap body 21 in which an inlet 30 and a collection port 40 are formed separately. The inlet 30 and collection port 40 each have a duckbill valve structure in which slits 33 and 43 are formed at the bottom of a mortar-shaped recess, with the opening of the recess facing upward.

[0027] By providing separate injection port 30 and collection port 40, contamination of the centrifuge tube can be prevented. Furthermore, by equipping both the injection port 30 and collection port 40 with a duckbill valve structure, the injection needle can be easily guided along the recess to the slits 33 and 43. By inserting the injection needle into the slits 33 and 43, the sample can be injected or collected without introducing cap residue into the centrifuge tube, and air can be vented through the gap between the slit and the injection needle during sample injection. Moreover, when the injection needle is withdrawn after being inserted into the slits 33 and 43, the slits 33 and 43 close, so the contents will not leak out even if the centrifuge tube is accidentally tipped over. In addition, the base of the injection needle does not reach the slits 33 and 43 but remains in the mortar-shaped recess, so the base of the needle does not contaminate the inside of the centrifuge tube, and the sample inside the centrifuge tube does not adhere to the base of the needle.

[0028] While the duckbill valve structure is generally used as a backflow prevention valve, the inventors' knowledge has shown that the slit in the duckbill valve structure is suitable for the insertion port of an injection needle.

[0029] As shown in Figures 3A to 3C, the sampling port 40 is located on the central axis A0 of the tube body 10, and the center of the slit 43 and the center of the opening surface of the sampling port 40 coincide with this central axis A0. This makes it easy to collect the components located in the intermediate section 13 after centrifugation of the sample.

[0030] On the other hand, the injection port 30 is formed at a position off-center from the central axis A0 of the tube body 10, and the slit 33 of the injection port 30 faces the central axis A0 of the tube body 10 (i.e., the perpendicular bisector of the slit 33 intersects the central axis A0). The injection port 30 also has a pair of sloped surfaces 34a and 34b with the slit 33 as one side. The central axis A1 of the slit 33 is closer to the central axis A0 of the tube body 10 than the central axis A2 of the opening surface of the injection port 30, and the recessed shape of the injection port 30 is eccentric. Therefore, when injecting blood into the tube body 10, by inserting the injection needle into the slit 33 along the sloped surface 34b that is further away from the central axis A0 of the tube body 10, the injection needle N can be tilted with respect to the central axis A0 of the tube body 10, as shown in Figure 8A, and the tip of the injection needle N can reach the middle part 13 of the tube body 10. Therefore, blood can be filled into the tube body 10 without the formation of blood pools.

[0031] The cap 20 has an inlet plug 31 and a collection plug 41 in addition to the cap body 21. The inlet plug 31 and the collection plug 41 are connected to the cap body 21 by strip-shaped connecting parts 32 and 42, respectively. As shown in Figure 4, the inlet plug 31 is fitted into the inlet 30 and the collection plug 41 is fitted into the collection 40.

[0032] When providing the inlet stopper 31 and collection stopper 41 on the cap 20, it is preferable to position the inlet stopper 31 near the inlet 30 and the collection stopper 41 near the collection stopper 40 so that the inlet stopper 31 is automatically selected when plugging the inlet 30 and the collection stopper 41 is automatically selected when plugging the collection stopper 40. In this embodiment, the inlet stopper 31, inlet 30, collection stopper 40 and collection stopper 41 are arranged in a line. This prevents the wrong stopper from being fitted and improves ease of use.

[0033] Furthermore, in this embodiment, the cap body 21, the inlet plug 31, the connecting part 32, the collection port plug 41, and the connecting part 42 are integrally molded from flexible resin. By integrally molding these, the number of parts is reduced compared to when the inlet plug 31 and the collection port plug 41 are formed separately from the cap body 21, thereby reducing manufacturing costs.

[0034] When integrally molding the cap body 21, the inlet plug 31, the connecting part 32, the collection port plug 41, and the connecting part 42 with a flexible resin, suitable resins such as silicone, polyvinyl chloride, polyethylene, and polypropylene can be used, with silicone resin being preferable. Furthermore, the wall thickness d of the slits 33 and 43 is preferably 1 to 2 mm.

[0035] (Slide adapter) The slide adapter 50 is a bottomed cylindrical shape that screws onto the lower part 12 of the tube body 10, and can move vertically (in the direction of axis A) by advancing or retracting the screw. In the centrifugal separator tube 1 of this embodiment, the tube body 10 is inserted into the slide adapter 50 when screwing. That is, as shown in Figures 7A and 7B, a threaded portion 51 is formed on the inner surface of the slide adapter 50, and this threaded portion 51 screws onto a threaded portion 15 on the outer surface of the lower part 12 of the tube body 10. Below the threaded portion 51 of the slide adapter 50 is a smooth sliding contact area 52. In addition, the outer surface of the slide adapter 50 is provided with an anti-slip surface consisting of numerous vertical ribs 53.

[0036] The inner diameter D4 of the sliding contact area 52 is smaller than the inner diameter D3 of the threaded portion of the screw portion 51. When the screw portion 51 of the slide adapter 50 is screwed into the screw portion 15 of the tube body 10, the sliding contact area 52 slides against the sliding contact area 16 below the screw portion 15 of the tube body 10. Liquid tightness between the slide adapter 50 and the tube body 10 is ensured by these sliding contact areas 52 and 16 and a packing 60 fitted into a groove 17 near the lower end of the tube body 10.

[0037] By adjusting the position of the slide adapter 50 in the A0 axis direction after centrifugation of the blood, it becomes possible to position the buffy coat layer in the intermediate section 13, making it easier to collect the buffy coat layer.

[0038] In this position adjustment, as shown in Figure 8F, if the position of the slide adapter 50 is lowered too much, the threaded portion 51 of the slide adapter 50 will be positioned below the threaded portion 15 of the tube body 10, and there is a risk that the two will not screw together and will spin freely. However, even in this case, the liquid-tight seal between the slide adapter 50 and the tube body 10 is maintained by the packing 60, and unless the slide adapter 50 is pulled strongly downwards, the slide adapter 50 will remain attached to the tube body 10, thereby preventing liquid leakage from the tube body 10 and preventing the slide adapter 50 from unintentionally detaching from the tube body 10.

[0039] The slide adapter 50, like the tube body 10, is made of a transparent resin such as polycarbonate.

[0040] (How to use) The method for collecting the buffy coat layer from blood using the centrifuge tube 1 of this embodiment can be carried out as follows.

[0041] As shown in Figures 1A and 1B, when the centrifuge tube 1 is not in use, the inlet plug 31 is fitted to the inlet port 30 and the collection port plug 41 is fitted to the collection port 40.

[0042] First, 15 mL of the subject's venous blood is collected in a syringe containing a blood coagulant (ACD-A). Then, the inlet stopper 31 is removed from the inlet port 30 of the centrifuge tube 1, and as shown in Figure 8A, the injection needle N is guided along the slope 34b of the inlet port 30 toward the slit 33, and inserted into the tube body 10 through the slit 33. The collected blood, 13.5 to 14.0 mL, is then injected into either the male-female line 18a or line 18b shown in Figure 1A. By moving the injection needle N along the slope 34b of the mortar-shaped recess toward the slit 33, it is easy to tilt the injection needle N relative to the central axis A0 and insert it into the slit 33. Furthermore, by positioning the tip of the injection needle N in the middle section 13, the tube body 10 can be filled with blood without clogging the middle section 13.

[0043] Next, as shown in Figure 8B, after filling with blood S, the inlet stopper 31 is fitted onto the inlet port 30, and the collection port 40 is left with the collection port stopper 41 in place, and the mixture is mixed by inverting the container.

[0044] Next, the inlet port 30 is closed with the inlet port stopper 31, and the collection port 40 is closed with the collection port stopper 41, and the mixture is centrifuged. For example, the centrifugation conditions are 1800G for about 4 minutes per cycle. This separates the blood in the tube into a plasma layer P, a buffy coat layer B containing platelets, and a red blood cell layer R. As shown in Figure 8C, if the buffy coat layer B is located in the middle section 13 and can be collected, the collection port stopper 41 is removed from the collection port 40 as shown in Figure 8G, and an injection needle (non-bevel needle) N2 is inserted into the slit 43 of the collection port 40 to collect the buffy coat layer B. Centrifugation may be repeated as needed.

[0045] If the buffy coat layer B is not located in the middle section 13 and is difficult to collect, the slide adapter 50 is raised (Figure 8D) or lowered (Figure 8E) relative to the tube body 10 to move the buffy coat layer B to the center of the middle section 13, and then the buffy coat layer B is collected by inserting the needle N2 into the collection port 40 as shown in Figure 8G.

[0046] (Transformation patterns) The present invention can take on various modified forms, and the modified parts can be combined as appropriate.

[0047] When a cap is provided on the tube body 10, instead of providing slits 33 and 43 at the bottom of the mortar-shaped recess, a round hole with a diameter larger than the needle diameter may be provided. In that case, it is preferable that the inlet 30 is sealed with the inlet stopper 31 and the collection stopper 40 is sealed with the collection stopper 41.

[0048] The cap may be provided with a check valve for releasing pressurized air from the tube body 10. Examples of check valves for releasing air include a duckbill valve formed in the cap body 21 such that its mortar-shaped recess faces inward into the tube body 10, or a slit-cut valve.

[0049] If an air venting check valve is provided, there is a risk that blood may leak out of the air venting check valve when blood in the tube body flows towards the air venting check valve. Therefore, it is preferable to cover the air venting check valve with a lid or stopper. [Industrial applicability]

[0050] When PRP is collected from blood using the centrifuge tube of the present invention, highly concentrated PRP with a platelet concentration effective for treatment can be obtained stably in a clean state. Furthermore, since the centrifuge tube of the present invention can be manufactured at low cost, it will be widely accepted in research and clinical applications.

[0051] The centrifuge tube of the present invention can be used not only for collecting PRP from blood, but also for centrifugation of any sample. [Explanation of Symbols]

[0052] 1, 1B Centrifugal tube 10 Tube body 11 Top 11a Upper inner slope 12 Lower part 13. Middle section 14 fins 15 Threaded part 16 Sliding contact area 17 Groove 18a Women's line 18b Men's Line 20 caps 21 Cap body 30 Inlet 31 Inlet plug 32 Connecting part 33 slits 34a, 34b slope 40 Sampling port 41 Collection port plug 42 Connecting part 43 slits 50 Slide Adapters 51 Threaded part 52 Sliding contact area 53 Anti-slip 60 packing 61 Rounded ring-shaped protrusion 62 Rounded ring-shaped protrusion A0 Tube body axis, central axis A1 Central axis of the slit in the injection port A2 Central axis of the opening surface of the inlet d. Thickness of the slit portion B. Buffy coat layer D1 Outer diameter of the threaded portion of the tube body D2 Outer diameter of the sliding contact area of ​​the tube body Inner diameter of the threaded portion of the D3 slide adapter Inner diameter of the sliding contact area of ​​the D4 slide adapter N Syringe needle N2 Non-bevel needle P plasma layer R red blood cell layer S blood

Claims

1. A centrifugal separator having a tube body and a bottomed cylindrical slide adapter into which the lower part of the tube body is inserted, The tube body and the slide adapter have a threaded portion, and below the threaded portion there is a sliding contact area where they slide against each other. The tube body has a groove near the lower end of the sliding contact area and a packing fitted into the groove. In a slide adapter, the inner diameter of the threads in the screw portion is larger than the inner diameter of the sliding contact area. Centrifugal separator.

2. The centrifugal separator tube according to claim 1, wherein the length of the threaded portion of the tube body forming the screw portion is shorter than the length of the threaded portion of the slide adapter forming the screw portion, with respect to the axial length of the tube body.

3. The centrifugal separator tube according to claim 1 or 2, wherein the tube body has a narrower inner diameter in the middle section compared to the upper and lower sections in the longitudinal direction, and fins are formed radially around the middle section.

4. A centrifuge tube equipped with a flexible resin cap covering the upper end opening of the tube body, wherein the cap has a cap body formed with an injection port for injecting a sample and an extraction port for collecting a predetermined component with a needle after centrifugation of the sample, an injection port stopper covering the injection port, and an extraction port stopper covering the extraction port, wherein the injection port and the extraction port each have a slit at the bottom of a mortar-shaped recess, and the central axis of the slit of the injection port is closer to the central axis of the tube body than the central axis of the opening surface of the recess of the injection port.

5. The centrifugal separator tube according to claim 4, wherein the cap body, the inlet plug, and the collection port plug are integrally molded.

6. The centrifugal separator tube according to claim 4, wherein the cap has a collection port on the central axis of the tube body and an injection port at a position off-center from the central axis.

7. The centrifugal separator tube according to claim 4, wherein the cap has a check valve for releasing pressurized air from the tube body.

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