Composition for reducing wheat allergen activity

Abstract

To provide a composition for reducing the activity of wheat allergen.SOLUTION: The present invention provides a composition for reducing the activity of wheat allergen that contains polyvinylpyrrolidone iodide.SELECTED DRAWING: None

Description

[[Technical Field]]

[0001] The present disclosure relates to compositions for reducing wheat allergen activity and the like. [[Background Art]]

[0002] In recent years, many allergic diseases such as atopic dermatitis, bronchial asthma, and allergic rhinitis have become problematic. Allergic diseases are caused, for example, by ingesting food containing allergens (food allergy), and may also be caused by allergens present in the environment (e.g., in the air or in residences). More specifically, for example, the disease may occur by inhaling an allergen present in the air, or by contacting an allergen present on objects (such as doors and walls) present in a residential space. For this reason, removal of allergens from the environment and reduction of allergen activity are also considered important. To date, in order to reduce allergen activity in the environment, for example, preparations having an activity of reducing the activity of dust mite allergens and pollen allergens have been developed (Patent Documents 1 to 5). However, regarding food allergens, there has been little recognition that they exist in the environment, and there have been almost no reports on components having an effect of reducing the activity of wheat allergens, which is one type of food allergen. Therefore, development of a method for reducing the activity of wheat allergens (particularly wheat allergens present in the environment) has been desired. [[Prior Art Documents]] [[Patent Documents]]

[0003] [[Patent Document 1]] Japanese Patent No. 6533853 [[Patent Document 2]] Japanese Patent No. 5574409 [[Patent Document 3]] Japanese Patent No. 4157692 [[Patent Document 4]] International Publication No. WO 2009 / 044648 [[Patent Document 5]] Japanese Patent No. 6721040 [Overview of the project] [Problems that the invention aims to solve]

[0004] The object of this disclosure is to provide a composition that reduces the activity of wheat allergens (particularly wheat allergens present in the environment). [Means for solving the problem]

[0005] The inventors discovered that polyvinylpyrrolidone iodide can reduce the activity of wheat allergens and have further improved the invention.

[0006] This disclosure includes, for example, the following subjects: Section 1. A composition containing polyvinylpyrrolidone iodide for reducing wheat allergen activity. Section 2. The composition according to item 1, containing 0.01% by mass or more of polyvinylpyrrolidone iodide. Section 3. The composition according to item 1 or 2, wherein the wheat allergen is gluten. Section 4. The composition according to claim 1 or 2, wherein the wheat allergen is a wheat allergen in the environment. [Effects of the Invention]

[0007] It can reduce the activity of wheat allergens. [Modes for carrying out the invention]

[0008] The embodiments included in this disclosure are described in further detail below.

[0009] The compositions included in this disclosure include polyvinylpyrrolidone iodide. In this specification, such compositions may be referred to as "the compositions of this disclosure."

[0010] Polyvinylpyrrolidone iodide (PVP-I) is a complex of 1-vinyl-2-pyrrolidone polymer (polyvinylpyrrolidone) and iodine, and is also known as povidone-iodine. Polyvinylpyrrolidone iodide is a representative disinfectant widely used in mouthwashes, hand sanitizers, and wound disinfectants, and is therefore considered to have excellent safety.

[0011] The content of polyvinylpyrrolidone iodide in the composition of this disclosure is not particularly limited as long as it can reduce the activity of wheat allergens. For example, it can be 0.01% by mass or more. The upper or lower limit of this range may be, for example, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.12, 0.14, 0.16, 0.18, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or about 1% by mass. More specifically, for example, it may be about 0.01 to 1% by mass, and preferably 0.02 to 0.5% by mass.

[0012] The compositions of this disclosure can reduce the activity of wheat allergens, as shown in the examples described later. Examples of wheat allergens include albumin, globulin, gliadin, glutenin, and gluten, with gliadin, glutenin, and gluten being preferred. Gliadin is classified into four types, α, β, γ, and ω, by electrophoresis, and glutenin is classified into high molecular weight (HMW) subunits and low molecular weight (LMW) subunits. The wheat allergens of this disclosure preferably include any of the above. Note that gluten is formed when gliadin and glutenin are hydrated to form a network structure. In other words, gluten contains gliadin and glutenin. The target wheat allergen may be a single type or a combination of two or more types.

[0013] Furthermore, in recent years, wheat allergens have been reported to be present in the environment. For this reason, the compositions of this disclosure can be suitably used to reduce the activity of wheat allergens in the environment.

[0014] The compositions of this disclosure contain polyvinylpyrrolidone iodide and may further contain other components. Examples of such other components include solvents, carriers, dispersants, emulsifiers, buffers, stabilizers, excipients, binders, disintegrants, lubricants, thickeners, surfactants, antioxidants, preservatives, antiseptics, disinfectants, coatings, colorants, fragrances, pH adjusters, and other anti-allergen components. These components may be used individually or in combination of two or more.

[0015] Examples of the solvent include water; alcohols such as methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, tert-butanol and n-hexanol; glycol ethers such as ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, polyoxyethylene glycol 200, polyoxyethylene glycol 400, propylene glycol, dipropylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, ethylene glycol monohexyl ether, ethylene glycol mono-2-ethylhexyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monohexyl ether, diethylene glycol mono-2-ethylhexyl ether, triethylene glycol monobutyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol mono-n-propyl ether, propylene glycol mono-n-butyl ether, dipropylene glycol monomethyl ether, dipropylene glycol monoethyl ether, tripropylene glycol monomethyl ether, 3-methoxybutanol, and 3-methyl-3-methoxybutanol. Among these, water or alcohols are preferred. These solvents may be used alone or in combination of two or more thereof.

[0016] The composition of the present disclosure can be prepared by a conventional method by combining iodinated polyvinylpyrrolidone with other optional components.

[0017] The shape of the composition of the present disclosure is not particularly limited. For example, the composition may be in the form of liquid (e.g., solution, suspension, etc.), paste, gel, cream, or solid (e.g., powder, granule, etc.). Among these, a liquid form is preferred.

[0018] In recent years, it has been reported that wheat allergens exist in the environment. Therefore, the composition of the present disclosure can be used by, for example, attaching it to an object that may contain wheat allergens by spraying, coating, dipping, or the like.

[0019] Objects that may contain wheat allergens are not particularly limited, and examples include clothing, interior items such as carpets, sofas, wallpaper, and curtains, bedding such as futon base fabrics, futon covers, futon batting, sheets, pillowcases, and mats, automotive parts such as car seats, car mats, ceiling materials, and floor materials, and fibers and fiber products such as stuffed toys, wet cleaning wipes, masks, filter materials, and dust collection bags for vacuum cleaners.

[0020] In addition, the composition of the present disclosure can also be added to detergents, fabric softeners and the like used during laundry.

[0021] The activity reducing effect of the composition of the present disclosure on wheat allergens (more specifically, wheat allergens in the environment) was confirmed for the first time by the evaluation method described in the Examples below (more specifically, a method of reacting wheat allergens with anti-wheat allergen IgE antibodies in serum that react with wheat allergens collected from the blood of allergy patients).

[0022] In this specification, the term "comprising" also includes "consisting essentially of" and "consisting of". In addition, the present disclosure includes all arbitrary combinations of the constituent features described in the present specification.

[0023] In addition, various characteristics (properties, numerical values, functions, etc.) described for each embodiment of the present disclosure described above may be combined in any way when specifying the subject matter encompassed by the present disclosure. That is, the present disclosure includes all subject matter consisting of any combination of each combinable characteristic described in the present specification.

Examples

[0024] The contents of this disclosure will be specifically explained using the following examples. However, this disclosure is not limited in any way to these examples. Unless otherwise specified below, the experiments were conducted under atmospheric pressure and room temperature (approximately 20-30°C). Unless otherwise specified, "%" means "mass%". Also, unless otherwise specified, the blending amounts of each component listed in each table are also in "mass%".

[0025] The inactivation (activity reduction) rate of wheat allergens was measured using a competitive ELISA method. Wheat allergen (gluten) was prepared to a concentration of 1 μg / mL, added to an ELISA plate, and allowed to stand overnight at 4°C to solidify (gluten-solid-phase plate). Gluten and the drug were mixed in a 9:1 ratio to an allergen concentration of 10 μg / mL and reacted overnight at 4°C (allergen reaction solution). A control was used where purified water was used instead of the drug. The obtained allergen reaction solution and anti-gluten IgE antibodies in serum that react with gluten, recovered from the blood of allergic patients, were added to the gluten-solid-phase plate, and a competitive reaction was performed. Anti-gluten IgE antibodies in serum bound to gluten solidified on the plate were bound to enzyme-labeled anti-IgE antibodies, and the luminescence of the substrate was detected.

[0026] Since anti-gluten IgE antibodies in serum do not bind to inactivated gluten, we determined that the higher the amount of serum antibodies that bind to the gluten immobilized on the plate (higher luminescence intensity), the greater the inactivation effect.

[0027] The following chemicals were used: 0.01% aqueous solution of polyvinylpyrrolidone iodide (PVP-I), 0.02% aqueous solution of PVP-I, 0.05% aqueous solution of PVP-I, 0.1% aqueous solution of PVP-I, 0.5% aqueous solution of PVP-I, 55% aqueous solution of ethanol (55% EtOH), 100% ethanol (100% EtOH), and 0.01% aqueous solution of tannic acid. Since browning of the chemicals occurs when the amount of tannic acid is increased, a concentration of 0.01% of tannic acid was selected to avoid browning.

[0028] The gluten concentration that shows 50% of the fluorescence intensity of the control is set to a 50% inhibitory concentration (IC). 50 ) and informed consent for medication 50 The relative IgE binding titer and allergen inactivation rate were calculated using the following formula.

[0029] Inhibition (%) = (Control fluorescence intensity - Drug fluorescence intensity) / (Control fluorescence intensity - Blank fluorescence intensity) × 100 IgE binding relative titer = control IC 50 / Medical IC 50 Inactivation rate (%) = (1-IgE binding relative titer) × 100 The results are shown in Table 1.

[0030] [Table 1]

[0031] ▲ indicates a negative value. As shown in Table 1, polyvinylpyrrolidone iodide was confirmed to have a high activity-reducing effect on gluten. On the other hand, tannic acid, which is known to reduce allergen activity against dust mites and cedar pollen, did not show any activity-reducing effect on gluten. Furthermore, ethanol, which is generally known to have protein-denaturing effects, also did not show any activity-reducing effect on gluten.

Claims

1. A composition containing polyvinylpyrrolidone iodide for reducing wheat allergen activity.

2. The composition according to claim 1, comprising 0.01% by mass or more of polyvinylpyrrolidone iodide.

3. The composition according to claim 1 or 2, wherein the wheat allergen is gluten.

4. The composition according to claim 1 or 2, wherein the wheat allergen is a wheat allergen in the environment.

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