Time-delay mite trap and integrated pest control method using the same

Abstract

To provide beekeeping technique to collect pure honey with minimal impurities and chemical contamination through effective integrated eradication and control of varroa mites in the beekeeping industry by a simple method avoiding complexity without requiring labor and without requiring cost / power such as installation of a mechanical device and minimizing use of chemical substances such as pesticides.SOLUTION: A time-delayed mite trap control method uses a honeycomb board, a honeycomb frame, or a honeycomb foundation frame whose depth dimension is two-thirds to one-third of the depth dimension of the inside of a honeybee rearing box, as a mite trap. A time-delayed mite trap is characterized in that honeybees are reared in order of a step 1 to a step 5 so as to be able to eradicate varroa mites. There is also provided an integrated pest control method using the time-delayed mite trap.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] The present invention relates to a technical invention in the apiculture industry for breeding honeybees and collecting honey, and particularly relates to a method for controlling Varroa destructor parasitizing honeybees and a beehive structure for control.

Background Art

[0002] In the apiculture industry, honeybees are bred using artificial hives such as beehives (breeding hives), and honey produced in the artificial hives is collected. These beehives are provided with a closed internal space except for the hive entrance, which serves as the access for honeybees. A plurality of combs, hive frames, foundation sheets, and hive frames fitted with foundation sheets (foundation frames), such as 8 to 10 in number, are arranged side by side therein, and the structure allows honeybees to form brood cells, which are nests, on these combs and hive frames. Of course, there are also beehives where 10 to 20 or more combs, hive frames, foundation sheets, and foundation frames are arranged.

[0003] The following detailed description of the invention will be made with reference to the accompanying drawings. In the description of the present invention, names of special structures in beehives for apiculture and special names related to honeybees are set forth, and similarly, only an outline of necessary matters regarding the habits of honeybees and the habits of mites in apiculture is described.

[0004] A foundation sheet is a thin sheet made of beeswax (officially it is called "bee wax", but it is referred to by the common name "beeswax" in the present application) with a honeycomb structure engraved thereon, and it is also available in plastic. The use of foundation sheets allows honeybees to build brood cells more quickly. There are foundation sheets for female bees with small-sized hexagonal honeycomb structures and foundation sheets for male bees with large-sized hexagonal honeycomb structures. Combs and hive frames refer to wooden frames for fitting and fixing foundation sheets and base plates for nests, and a frame with a foundation sheet fitted therein is called a foundation frame. (After brood cells (honeycombs) are formed, the entire hive frame including the brood cells may be referred to as a comb.) Brood cells are honeycomb-shaped nest holes for honeybee larvae, and they are also places for storing honey and pollen. The brood cells of male bees are larger than those of worker bees, and the brood cells of queen bees are even larger, which are also called queen cells.

[0005] In Japan and around the world, Western honeybees are the most commonly used species for beekeeping. Western honeybees, used in beekeeping, have characteristics such as being able to produce large quantities of honey and being easy to maintain large colonies. Japanese honeybees, on the other hand, produce less honey compared to Western honeybees and are more prone to swarming (swarming is the behavior in which the queen bee and about half of the worker bees leave their nest to find a new nesting site) and absconding. For these reasons, they are rarely used in beekeeping. However, many people prefer the taste and quality of Japanese honeybee honey, so small-scale beekeeping of Japanese honeybees is also practiced.

[0006] Although this application primarily describes the invention in relation to Western honeybees, it is hereby stated that the invention is also valid for Japanese honeybees.

[0007] Generally, honeybee colonies consist of one queen bee and many worker bees. The queen bee continues to lay eggs in the nest, and these eggs mainly become worker bees. Worker bees are divided into brood bees, which secrete beeswax to build honeycomb (honeybee nests with formed cells), and which specialize in maintaining the nest and caring for the larvae, and honey collectors, which specialize in collecting pollen and nectar, processing it into honey, and storing it. However, in this application, honey collectors are also referred to as worker bees to distinguish them from brood bees. Furthermore, brood bees build large honeycomb cells called queen cells in the honeycomb, feed the larvae royal jelly, and raise the next generation of queen bees.

[0008] Beeswax is a wax secreted from the glands of worker bees, used to build honeycomb cells and to make honeycomb caps and cell lids.

[0009] Among the mites that harm honeybees, the most dangerous is the Varroa mite, and this application primarily focuses on the Varroa mite, which will be described below. However, it should be noted that this application does not exclude other mite species that have similar habits to those of the Varroa mite used in this application.

[0010] When honeybees are infested with the Varroa mite, the mite eats the fatty tissue in the bee's abdomen, which is essential for insects. This leads to malnutrition, impaired energy metabolism, and weakened immunity, ultimately resulting in fatal consequences.

[0011] Furthermore, the impact of mites that parasitize honeybees is largely due to mite-borne viral infections. Nearly 20 types of mite-borne viral diseases are known. In particular, five types of viruses have been proven to be transmitted by the Varroa mite: cashmere virus, sacbrood virus, acute paralysis virus, Israeli acute paralysis virus, and wing deformity virus.

[0012] Western honeybees are particularly vulnerable to mites that transmit these viruses, especially the Varroa mite, and male bees are especially susceptible. This is because male Western honeybees have a long pupation period, allowing mites that infiltrate the honeycomb cells to reproduce and grow. Japanese honeybees have a shorter pupation period, so they are relatively less affected by Varroa mites. Western honeybees are currently the dominant species in the beekeeping industry in Japan, and the damage caused by Varroa mite infestations is extensive. In particular, there have been reports of an increase in highly virulent viruses in recent years.

[0013] Although there are several scientifically classified types of the Varroa mite mentioned above, in this application, all of them will be referred to collectively as Varroa mites. Furthermore, in this application, Varroa mites will also be referred to simply as mites.

[0014] Currently, three types of mite control agents are registered in Japan. Apistan has been on the market for 27 years, and it is said that mites have already developed strong resistance to it. Apivar is said to be less effective in hot and humid regions like Japan, and it is also said that mite resistance is increasing. Timovar is said to have similar drawbacks. In addition, some types of control agents can reduce the quality of beeswax collected from honeybees. Due to these problems, the beekeeping industry has not yet been able to develop effective countermeasures against mite infestation and reproduction. In recent years, there has been an increase in cases where honeybees that beekeepers have painstakingly raised are wiped out due to mite infestation.

[0015] In beekeeping, mite infestations are devastating, and various countermeasures have been developed over the years. While insecticides have been used, as mentioned above, they are by no means foolproof. Beekeepers are eager for technologies or methods that can completely eradicate mites without the need for insecticides, which can lead to mite resistance development and a decline in beeswax quality.

[0016] Traditionally, various proposals have been made in response to such requests, based on several prior documents listed below.

[0017] For example, Patent Document 1 (Japanese Patent Publication No. 2021-40535) contains the following technical disclosure.

[0018] In other words, there is a technical disclosure regarding a pest control device comprising: a housing having a first opening and a second opening formed therein; an intake unit detachably attached to the housing and forcibly drawing outside air into the housing through the first opening; a retention unit provided inside the housing in communication with the first opening and for temporarily retaining the outside air taken in by the intake unit; and a pest control component generating unit provided inside the housing in communication with the retention unit and the second opening and for generating pest control components.

[0019] However, the pest control device described in Patent Document 1 is a mechanical device that forcibly takes in outside air and temporarily retains that outside air inside, requiring a complex device system and the power (electricity) needed to drive it. Furthermore, it requires a unit that generates pest control components inside, which also requires power. In short, it is an expensive and precise mechanical device, and in the beekeeping industry, hundreds of beehives (breeding boxes) are sometimes handled, so considering the cost and workability of the above device, it is not practical.

[0020] In other words, the technical disclosure in the above-mentioned document 1 does not come close to solving the problem of the present invention, which aims to develop an effective mite control method that is simple, inexpensive, and not complicated.

[0021] Furthermore, Patent Document 2 (Japanese Unexamined Patent Publication No. 2006-124377) discloses a parasite control agent composition for honeybee colonies containing fenpyroximate as an active ingredient, and a method of using the control agent composition by bringing the active ingredient into contact with honeybees.

[0022] The aforementioned Patent Document 2 discloses a parasite control composition, i.e., a pesticide, and differs in technical concept from the present invention, which aims to effectively control mites in beekeeping by minimizing the amount of mite control agent used. In beekeeping, there is great value in collecting and providing honey that is as free as possible from impurities and drugs such as pesticides, and Patent Document 2, which presupposes the use of pesticides from the outset for mite control, is incompatible with this. Furthermore, as already described, pesticides have the major disadvantage of causing mites to develop resistance, creating mites that have developed resistance to the pesticide after many years of use.

[0023] Utility Model Document 3 (Japanese Utility Model Registration No. 3196081 Publication) relates to a honeybee parasitic mite extermination tool, and particularly relates to a honeybee parasitic mite extermination tool containing a mite extermination component such as an acaricide or a mite-proofing agent. The present disclosure relates to the technology of a honeybee parasitic mite extermination tool characterized by comprising a sheet body including a sheet member containing one or more materials selected from the group consisting of a paper sheet having a mite extermination component, a woven fabric sheet, and a non-woven fabric sheet, and a mesh member covering a surface of the sheet member.

[0024] However, the above content of Patent Document 3 is also a technical disclosure of a parasite control composition, that is, an extermination agent, and the technical idea is to perform extermination by impregnating a sheet such as paper or non-woven fabric with the extermination agent and bringing the sheet into contact with honeybee parasitic mites. Similar to Document 2, the technical idea is different from that of the present invention, which aims to effectively control mites by minimizing the usage amount of the acaricide. To reiterate, in beekeeping, it is of great value to collect and provide pure honey that does not contain impurities, extermination agents, or the like as much as possible. Both Patent Document 2 and Patent Document 3 mentioned above, which are premised on the use of extermination agents from the beginning for mite control, are ideologically incompatible with the present invention. It should be emphasized that the heavy use of extermination agents goes hand in hand with the great risk of inducing resistance in mites and creating acaricide-resistant mites.

[0025] As described in the above Documents 1, 2, and 3, none of them is far from achieving the object and solving the problem of the present invention, nor do they even mention the problem of the present application.

[0026] An object of the present invention is to provide a method and a beekeeping system, which can effectively perform comprehensive extermination and control of Varroa destructor mites by a method as simple as possible in the beekeeping industry, without requiring labor from beekeepers, without incurring costs for large-scale equipment and the like, and additionally minimizing the use of chemical substances such as extermination agents, so as to collect pure honey with minimal contamination by impurities and chemical substances, and the object of the present invention is to develop the method and the beekeeping system. [Prior Art Documents] [Patent Documents]

[0027] [Patent Document 1] Japanese Unexamined Patent Application Publication No. 2021-40535 Patent Document 2 Japanese Unexamined Patent Application Publication No. 2006-124377 Patent Document 3 Utility Model Registration Publication No. 3196081 Summary of the Invention Problems to be Solved by the Invention

[0028] The present invention is intended to solve the above-mentioned problems and issues in the prior art. Specifically, the object of the present invention is to develop a beekeeping technique that can effectively perform comprehensive extermination and control of Varroa destructor mites in the beekeeping industry through a less complicated and as simple a method as possible, which does not require extra labor from beekeepers, does not incur costs such as installation of mechanical devices, additionally minimizes the use of chemical substances such as pesticides, and allows collection of pure honey with minimal contamination of impurities and chemical substances; and the object of the present invention is to develop and provide such a method and beekeeping system. In addition, the method for solving the above problems derived from the concept of Integrated Pest Management (IPM), which is the basic idea of the present invention for minimizing the use of extermination agents, is also described as one aspect of the present invention ( Claim 6 described herein). Furthermore, what is important in the present invention is that the invention can be conceived as a solution to the problem that makes maximum use of the natural ecology and natural habits of honeybees and Varroa destructor mites.

[0029] Herein, only necessary information regarding the habits of Varroa destructor mites, the target of extermination and control, is described below. (Refer to biological publications for details.)

[0030] Varroa mites (hereafter, mites including Varroa mites will be collectively referred to as "mites") parasitize both male and female honeybees. The scent (pheromones) of male honeybee larvae seems to be very attractive to mites that are about to lay eggs, and they readily burrow into any honeycomb cells containing male larvae. They enter just before the honeycomb cells containing male honeybee larvae are capped (the period just before the larvae pupate), and this timing is thought to be in order to recognize and identify changes in chemical substances (pheromones) emitted from the body surface of male honeybee larvae that are about to pupate. These chemical substances are said to be mainly composed of fatty acid esters, as well as carboxylic acids and hydrocarbon odorants.

[0031] Female mites that enter the cells of male honeybee larvae lay eggs, hatch, and develop into adult mites during the approximately 14 to 15 days between the time the cell is capped for the male bee larvae to pupate and the time they emerge as adults. There are 5 to 6 mites in a single cell, and as soon as the adult male honeybees emerge and leave the cell, they leave and spread to other honeybees (worker bees) by parasitizing them. Considering the number of male bee cells, this results in an enormous number of mites appearing.

[0032] Next, we will briefly describe only what is necessary for this invention regarding the habits of honeybees. (For details, please refer to biological textbooks.) Honeybees are classified into male bees, female bees (worker bees that raise the young, and worker bees that collect honey), and queen bees, and the duration of each stage from egg hatching to larval, pupal, and adult emergence differs. The period from when male wasp larvae cover their nest cells to when they pupate and emerge is approximately 14 to 15 days, with the pupal period lasting about 13 days. The period from when a female wasp larva covers its cell to when it pupates and emerges is approximately 12 to 13 days, with the pupal stage lasting about 10 days. Incidentally, the period from when the queen bee larva is capped to when it pupates and emerges is about 6 to 7 days, and the interval between pupal stages is about 6 days.

[0033] As mentioned above, female mites that enter honeybee larval cells lay one egg daily for about three days after intrusion, during the period from when the honeybee larvae are capped for pupation until they pupate and emerge. These eggs develop into adult mites in about six days. One female mite in a male honeybee cell, which has the longest pupal period, can produce about six adult mites. As soon as the honeybee pupae emerge, the mites also leave the cell and parasitize other honeybees. Unhatched mite eggs in the honeybee cells die after the honeybees emerge.

[0034] In other words, considering the difference in the time from cell capping to emergence between male and female honeybee larvae as described above, it is most advantageous for mites to enter the cells of male bee larvae, which have a longer pupal period, and lay their eggs there. Therefore, it is thought that in nature, mites have developed the habit of sensing the chemical substances emitted when male bees pupate and preferentially entering the cells in which those male bees are pupating.

[0035] The key aspect of this invention lies in the fact that it devised a means of solving the problem in accordance with natural ecology by making maximum use of the ecology and habits of honeybees and mites described above to control and eliminate mites. Furthermore, many other aspects also make maximum use of the habits of honeybees and mites, and these will be described and explained as they arise. [Means for solving the problem]

[0036] To solve the aforementioned problems, the present invention provides a time-delay type mite trap and a comprehensive pest control method using the same, which is installed inside a beehive that serves as the mite trap. honeycomb, frame, or foundation frame The invention and its honeycomb, frame, or foundation frame This invention consists of two parts: a product and a method, which are an invention of a comprehensive pest control method for mites using a time-delay mite trap.

[0037] (Claim 1) The time-delay mite trap and the integrated pest control method using the same according to claim 1 of the present invention are inserted inside a beehive in beekeeping. honeycomb, frame, or foundation frame And, as mentioned above honeycomb, frame, or foundation frameThe depth dimension is two-thirds to one-third of the depth dimension inside the beehive, and the foundation frame is a foundation for female bees, honeycomb, frame, or foundation frame The lower part makes it easier for worker bees to build male bee cells. In addition, a container containing a mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation capping, is provided near the connection point between the aforementioned honeycomb board or frame or foundation frame and the aforementioned frame of any size or beeswax-based male bee foundation. A time-delayed mite trap with distinctive features, and used in an integrated pest control method employing it. honeycomb, frame, or foundation frame This is an invention.

[0038] Beehives used in beekeeping honeycomb, frame, or foundation frame This will be explained in detail in Figure 1.

[0039] Used in a standard beehive (hereinafter also referred to as a beehive) honeycomb, frame, or foundation frame The dimensions are made according to the internal dimensions of the breeding box; the depth is made to match the depth of the breeding box, and the width is made to match the width of the breeding box. honeycomb, frame, or foundation frame The width of the comb is arranged in the hive to a desirable size (approximately 8mm to 9mm between combs) considering the necessary space width, in order to maintain a bee density that allows for easy bee activity, the thickness of the honeycomb cells produced, and the overall efficient density within the hive. In a standard hive, within the internal length dimension... honeycomb, frame, or foundation frame The structure is designed to hold 10 sheets, but as long as the necessary spacing between them is maintained as described above, the number of sheets arranged may be 10 or more, or 10 or less. Therefore, the important thing is the conventional use of beehives. honeycomb, frame, or foundation frame The depth dimension is approximately the same as the internal depth dimension of the breeding box.

[0040] However, the depth dimension of the comb, frame, or foundation frame used in the time-delay mite trap and the integrated pest control method using the same according to claim 1 is two-thirds to one-third of the depth dimension inside the beehive, and is characterized by having a space below it. An important technical feature of this is that it makes it easier for worker bees to habitually build slightly larger male bee cells.

[0041] Worker bees have a habit of energetically creating honeycomb cells to raise worker bee (female bee) larvae in order to increase the number of bees in the hive (to strengthen the honeybee colony), and a certain number of worker bee cells will be lost to other honeycomb or hive frame Once secured, they have the habit of creating slightly larger comb cells to raise male bee larvae for the purpose of mating with the queen bee. Therefore, the comb board, frame, or foundation frame used in the time-delay mite trap and the integrated pest control method using the same according to the present invention has a special structure according to the present invention, which is made to be two-thirds to one-third of the depth of the hive, and is intended to allow sufficient worker bee (female bee) comb cells to be created in advance in that part, and then allow the bee to create comb cells to raise male bee larvae for use as a mite trap in the space of one-third to two-thirds of the depth of the hive below.

[0042] In other words, in the comb, frame, or foundation frame used in the time-delay mite trap and the integrated pest control method using the same according to claim 1, when foundation is used in the comb, frame, or foundation frame portion, it is preferable to use foundation for worker bees (female bees). Similarly, other materials not used in the time-delay mite trap of the same breeding box honeycomb, frame, or foundation frame It is preferable to pre-install all worker bee (female bee) foundations, as this creates a situation where there is a shortage of male bee cells. This is because, when a comb, frame, or foundation frame used in the time-delay mite trap and the comprehensive pest control method using the same according to the present invention as described in claim 1 is inserted, the honeybees are more likely to actively create only male bee cells that will serve as mite traps in the space below.

[0043] In summary, in other combs, frames, or foundation frames not used in the time-delay mite trap and integrated pest control method using the present invention, worker bees actively create a large number of worker bee (female bee) cells. Therefore, the lower space of the comb, frame, or foundation frame used in the time-delay mite trap and integrated pest control method using the present invention cleverly utilizes the bees' habit of making it easier for male bee cells to be created.

[0044] In the time-delay mite trap and integrated pest control method using the same, the queen bee lays eggs in the male bee cells made in the comb, frame, or foundation frame used in the present invention. When the larvae hatch from the queen bee and grow from the first to the fifth instar, they pupate and emit a substance mainly composed of fatty acid esters (scent components of fatty acid esters, carboxylic acids, and hydrocarbons) from her body, instructing worker bees to cap the cells. This substance is a mite attractant, and mites, especially Varroa mites, are attracted and enter the male bee cells in large numbers, after which the cells are capped. Of course, during the male bee pupa period after the cells are capped, the male pupae are parasitized by mites, and the mites actively lay eggs, hatch, and reproduce within the cells.

[0045] In other words, as described above, the mite trap invention of the time-delay mite trap and the integrated pest control method using the same according to the present invention involves a mechanism in which, using a comb, frame, or foundation frame, male bee larvae emit mite-attracting substances in male bee cells concentrated in a specific part of the beehive, attracting a large number of mites and trapping them within the male bee cells.

[0046] Claim 1 The time-delay mite trap and the integrated pest control method using the same described herein are used in the integrated pest control method using the time-delay mite trap. honeycomb, frame, or foundation frame However, it is inserted inside the beehive in beekeeping. honeycomb, frame, or foundation frame And, as mentioned above honeycomb, frame, or foundation frame The depth dimension of the foundation frame is two-thirds to one-third of the depth dimension of the inside of the beehive, and the foundation frame is a foundation for female bees, and further, honeycomb, frame, or foundation frame At the bottom, a frame of any size or made of beeswax to facilitate the construction of male bee cells. Male honeycomb foundation or beeswax-made male honeycomb foundation frame This invention relates to a time-delay type mite trap characterized by having one or more connected components, and to a comb, frame, and foundation frame used in an integrated pest control method using the same.

[0047] In other words, it is used in a comprehensive pest control method using a time-delay mite trap as described in claim 1. honeycomb, frame, or foundation frameThe following is the claim described in claim 1. honeycomb, frame, or foundation frame In the space below, one or more frames, beeswax drone beehives, or beeswax drone beehives frames of any size are connected together. For example, as shown in sections b and c of Figure 2 below, additional frames, beeswax drone beehives, or frames are installed in a continuous manner. There are no limitations on the size of the surfaces of the connected frames, beeswax drone beehives, or frames, nor on the number. Needless to say, they must be small enough to fit inside the hive. Also, if the connected frames, beeswax drone beehives, or frames are small, the area at the bottom that is not connected may remain empty.

[0048] Honeybees will actively build male bee cells in newly added frames or beeswax male bee foundations or foundation frames. If beeswax male foundations are present, honeybees will build male bee cells more actively and quickly. In addition, honeybees will also build male bee cells, albeit slightly later, in the spaces where no additional frames, beeswax male bee foundations, or beeswax male bee foundation frames have been added.

[0049] Claim 1 Used in the comprehensive pest control method using the time-delay mite trap described above. honeycomb, frame, or foundation frame If there are two additional frames or beeswax male beehive foundations or beeswax male beehive foundation frames attached to the bottom, as shown in parts b and c of Figure 2, each of them will be described later. Claim 4 This is a time-delay type mite trap as described. Claim 1 Used in the comprehensive pest control method using the time-delay mite trap described above. honeycomb, frame, or foundation frame If there is one additional frame, beeswax male beehive foundation, or beeswax male beehive foundation frame attached to the lower part, that one frame and the empty space without additional installation will be as described below. Claim 5 This is a time-delay type mite trap as described.

[0050] Claim 1 The time-delay mite trap and the integrated pest control method using the same described herein are used in the integrated pest control method using the time-delay mite trap. honeycomb, frame, or foundation frame However, it is inserted inside the beehive in beekeeping. honeycomb, frame, or foundation frame And, as mentioned above honeycomb, frame, or foundation frame The depth dimension of the foundation is two-thirds to one-third of the depth dimension of the inside of the beehive, and the foundation is for female bees, and further, honeycomb, frame, or foundation frame At the bottom, a frame of any size or to facilitate the construction of male bee cells. Beeswax male honeycomb base or beeswax male honeycomb base frame One or more of these are connected and provided, in addition, the above honeycomb, frame, or foundation frame and the aforementioned hive frame of any size Alternatively, a beeswax male honeycomb base or a beeswax male honeycomb base frame. A time-delay mite trap and an integrated pest control method using it are characterized by having a container containing a mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation and capping, located near the connection point. honeycomb, frame, or foundation frame This is an invention.

[0051] In other words, Claim 1 Used in the comprehensive pest control method using the time-delay mite trap described above. honeycomb, frame, or foundation frame The following is the claim described in claim 1. honeycomb, frame, or foundation frame And, attached to the lower part, a piece of any size Hive frames or beeswax-made male beehive foundations or beeswax-made male beehive foundation frames Near the connection point, a container is provided that contains a mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation.

[0052] As already mentioned, the mite-attracting compound is a chemical substance produced from the body surface of male bee larvae just before the honeycomb cells are capped, and is mainly composed of fatty acid esters and odor substances such as carboxylic acids and hydrocarbons. Many mites in the rearing box are attracted to this attractant compound, and the results described in claim 1 honeycomb, frame, or foundation frame They will actively invade the hive frame of any size, or the beeswax male beehive foundation or beeswax male beehive foundation frame, which are attached to the lower part of the hive. Then they will enter the male beehive cells.

[0053] The attractive effect of chemical substances emitted from a container containing mite-attracting compounds is, Claim 1In addition to the mite-attracting effect of attractant compounds emitted by male bee larvae in the cells built on the connected frames, beeswax-based male bee foundations, or foundation frames described above, the effect can be doubled as a synergistic effect to powerfully attract mites.

[0054] ( Claim 2 ) Claim 2 The time-delay mite trap and the comprehensive pest control method using the same according to the present invention as described below , claim 2 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame This invention relates to a time-delay mite trap control method that uses a mite trap, and is characterized by the fact that mites, particularly Varroa mites, can be eradicated by raising honeybees in the following order from Step 1 to Step 5. Step 1. Other inside the beehive (honeybee keeping box) honeycomb, frame, or foundation frame On one side of the array, Claim 2 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame Insert one or more of these as the first trap. Step 2. At the bottom of the first trap in Step 1, a male bee cell is created, and male bee eggs are laid by the queen bee, and when the larvae reach the 2nd to 4th instar stage, Other inside the aforementioned nest box honeycomb, frame, or foundation frame On the other side of the array, Claim 2 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame Insert one or more of these as a second trap. Step 3. Step 1: During the pupal stage of the male bee larvae in the male bee cells formed at the bottom of the first trap, the first trap is removed, the male bee cells formed at the bottom of the first trap are cut off and disposed of, and then the first trap is inserted back into its original position. Step 4. Step 2: During the pupal stage of the male bee larvae in the male bee cells formed at the bottom of the second trap, the second trap is removed, the male bee cells formed at the bottom of the second trap are cut off and disposed of, and then the second trap is inserted back into its original position. Step 5. Steps 3 and 4 described above are repeated.

[0055] Claim 2 The time-delayed mite trap and integrated pest control method using the same, as described in the present invention, is an invention of a mite extermination method using a first mite trap and a second mite trap in a time-delayed manner, and the method is shown in steps 1 to 5.

[0056] In other words, assuming that honeybees are constantly being kept inside the hive, that a large number of female bee cells are being produced, and that a large number of mites are also present, Claim 2 This is a method of attracting and eliminating mites. It is a trap that attracts and eliminates mites. Claim 2 The plan is to use the comb, frame, or foundation frame described above as the first and second mite traps, and to create a time difference by staggering the periods during which the mite collection and extermination functions of both mite traps are activated, thereby continuously attracting and eliminating mites within the hive.

[0057] In Step 1, for example, at one end of the hive where 10 comb frames or foundation frames can be inserted, the first trap is placed as Claim 2 Insert the comb, frame, or foundation frame as described above. The bees will then actively begin building male bee cells in the space below the comb, frame, or foundation frame, in the space that extends one-third to two-thirds of the way from the bottom of the hive, and the queen bee will begin laying male bee eggs, hatching them, and developing the larvae.

[0058] Here, we define one cycle of using a comb, frame, or foundation frame as the period from the moment the comb, frame, or foundation frame is inserted into the hive, that is, from the insertion of the first trap, through the formation of male bee cells, queen bee egg-laying, male bee larvae hatching, growth from first instar to fifth instar larvae, capping of the cells, pupation, and the pupal period. Then, for about half of this cycle, the male bee larvae are growing, and most of the larvae are in the second to fourth instar stage.

[0059] Insects, including honeybees, grow larger after hatching by repeatedly molting during their larval stage before pupating. Including hatching, they molt five times before pupation, and in entomology, the number of molts is used to express the stage of larval development (instar). After hatching, they are called 1st instar larvae, then they molt to become 2nd instar larvae, and so on, growing into 3rd, 4th, and 5th instar larvae (final instar larvae). After that, they pupate, and after the pupal period, they emerge as adults.

[0060] Of course, the numerous male bee larvae growing in each of the many male bee cells do not necessarily grow at the same rate simultaneously. Therefore, the period when most larvae are between the second and fourth instar stages is considered to be approximately half the duration of the aforementioned cycle. The present invention utilizes this half-cycle period, or one-half of the cycle duration, as the time difference for mite traps.

[0061] In other words, one cycle begins when the first trap is inserted, and the latter half of that cycle is the period during which the first trap functions as a mite trap to attract mites. The period from when the male bee larvae are in their 5th instar until the cells are capped is the period during which the mite trap functions as a mite trap. The next trap, the second trap, is inserted with a time delay as soon as the first half of the first trap's cycle is completed, and the second trap's cycle begins. Then, as soon as the first trap's cycle ends, the latter half of the second trap's cycle begins, and the second trap's mite-attracting period begins. By repeating this, the mite-attracting function of the mite traps is maintained almost constantly inside the hive. Claim 2 So, here it is.

[0062] Specifically, in Step 1, the first trap is inserted into the hive, and halfway through the cycle, in Step 2, the second trap is inserted on the opposite side of the hive. From this point onward, in the first trap, the male bee larvae begin to release mite-attracting compounds, attracting mites that enter the male bee cells, and the cells are sealed just before the larvae pupate. Meanwhile, in the newly inserted second trap, male bee cells begin to form, and then the queen bee lays eggs, the male bee larvae hatch, and the larval development begins.

[0063] Step 3 is the end of one cycle for the first trap mentioned above. The male bee pupae in the first trap are removed while they are still pupae, and the male bee cells, including any mites inside, are cut off and disposed of. Claim 2 The male bee cells (true trap portion) created in the space at the bottom of the comb, frame, or foundation frame, which occupies one-third to two-thirds of the depth of the hive, are made of beeswax by the bees and are therefore soft. They can be easily cut into any shape with a cutter or similar tool, then returned to their original position in the hive, and a new cycle begins as the first trap. Simultaneously, in the second trap, the male bee larvae begin to release mite-attracting compounds, attracting mites, which then begin to enter the male bee cells. Just before the larvae pupate, the brood bees seal the cells.

[0064] After Step 3 is completed, and the second trap's cycle is finished, with the male bee cells in the pupal stage, Step 4 involves removing the second trap, cutting out and disposing of all male bee cells, including any mites inside, and returning it to its original position in the hive. Simultaneously, in the first trap, the male bee larvae begin to release mite-attracting compounds, attracting mites that begin to invade the male bee cells. The cells are then sealed just before the larvae pupate. After that, Steps 3 and 4 are repeated every half of the cycle period described above. Claim 2 This invention relates to a time-delay type mite trap and a comprehensive pest control method using the same, as described in [reference].

[0065] Here, we will discuss the ecology and habits of honeybees. Regarding To add to this information: Male bee larvae are known to attract more Varroa mites than worker bee larvae. Inserting empty frames (wooden frames) or male bee foundations into a beehive will yield a large number of male bee larvae in a relatively short time. Mites are attracted to the substances these male bee larvae release before they cap their cells and prefer to invade the cells of the male bee larvae. When the male bee larvae pupate, they cap their cells. Once the entire male bee larva is capped, removing and discarding the capped cells will attract and eliminate the mites.

[0066] Honeybee larvae have their cells capped by brood bees (worker bees that specialize in childcare) shortly before pupating inside the honeycomb. Male bee larvae reach the peak of capping pheromones released from their body surface around 10 days after hatching, that is, when they are in the 5th instar, just before pupation. This pheromone is said to be the scent of fatty acid esters, carboxylic acids, and hydrocarbons secreted from the honeybee's body surface. This pheromone causes brood bees to cap the cells of male bee larvae. Similarly, this pheromone is also an attractant for mites (in this case, the bees that produce the attractant pheromone are harmed, so it is technically called a kairomone), and mites enter the cells of male bee larvae just before capping. be When comparing the larvae of female worker bees and male bees, male bee larvae secrete more of this attractant substance over a longer period, which is why mites prefer to invade the cells of male bee larvae (Troullier et al., 1992). In the honeybee population as a whole, mites reproduce most abundantly in males, followed by worker bees and then queen bees. One study (Fuchs S. 1992) reported that mites are 8 to 12 times more attracted to the cells of male bee larvae than to worker bee larvae. Furthermore, there is very little room for mites to enter and develop in the cells of queen bee larvae. This is due not only to the amount and duration of the mite-attracting pheromone, but also to the short pupal period of worker bees and queen bees after invading the cells. The above describes the mite-attracting method using male bee larvae (male larval attraction method).

[0067] Furthermore, the time spent covering the honeycomb cells of bee larvae differs significantly: 15-20 hours for worker bee cells, compared to 40-50 hours for male bee larvae cells. This means that mites have a longer period to invade the hive and a longer period for mites attached to worker bees to invade the honeycomb cells, resulting in a higher probability of invasion.

[0068] Mites that invade the honeycomb cells of bee larvae lay eggs, which hatch during the pupal stage when the honeycomb cell is sealed. The mites then parasitize the pupa and grow. Therefore, the amount of mite reproduction varies depending on the length of the bee's pupal stage. The number of mites increases within the bee's pupal cell.

[0069] The above is Claim 2 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame This is a time-delay mite trap control method that uses a mite trap. Claim 2 This invention cleverly utilizes the ecology and habits of honeybees and mites to continuously attract mites to the honeybee hive using two drone cell traps. This allows mites to be efficiently lured into the drone cells, and then removed and eradicated after the cells are capped.

[0070] (Claim 3) Claim 3 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 3 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame This invention relates to a time-delay mite trap control method that uses a mite trap, and is characterized by the fact that mites, particularly Varroa mites, can be eradicated by raising honeybees in the following order from Step 1 to Step 5. Step 1. Other inside the beehive (honeybee keeping box) honeycomb and / or frames or foundation frames On one side of the array, Claim 3 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame Insert one or more of these as the first trap. Step 2. Seven days after Step 1, Other inside the aforementioned nest box honeycomb, frame, or foundation frame On the other side of the array, Claim 3 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame Insert one or more of these as a second trap. Step 3. Seven days after Step 2, The first trap is removed, the male bee cells formed at the bottom of the first trap are cut off and disposed of, and then the first trap is inserted back into its original position. Step 4. Seven days after Step 3, Remove the second trap, cut off and remove the male bee cells formed at the bottom of the second trap, and then insert the second trap back into its original position. Step 5. Seven days after step 4, Steps 3 and 4 described above are repeated every 7 days.

[0071] Claim 3 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 2 Similarly, this invention describes a method of mite extermination using a first mite trap and a second mite trap in a time-delayed manner, and the method is shown in steps 1 to 5. However, Claim 3 The time-delay type mite trap and the comprehensive pest control method using the same, as described in Claim 4, have a noteworthy invention in addition to Claim 4. This is described below.

[0072] Honeybees, for example, begin their activity in early spring and soon after enter their breeding season, engaging in activities that dramatically increase their colony size over several months. Worker bees create honeycomb cells within the hive, the queen bee lays eggs, brood bees (worker bees) raise the larvae, and worker bees (honey harvesters) collect nectar and beeswax, all of which become extremely active. At the same time, mite infestations also thrive during this period, along with the development of honeybee larvae. This is also a very busy and complex time for beekeepers, involving tasks such as managing the hives and harvesting honey.

[0073] During this honeybee breeding season, worker bees quickly create cells and queen bees lay eggs. Because the cell creation period and queen bee egg-laying period are short, the entire cycle, from inserting a comb, frame, or foundation frame as a trap to the formation of male bee cells, queen bee egg-laying, male bee larvae hatching, growth from first-instar to fifth-instar larvae, cell capping, pupation, and pupal period, is shortened to approximately 14 days (about 2 weeks). Therefore, this cycle period is shortened by half, conveniently becoming about 1 week (about 7 days).

[0074] In other words, Claim 3 In the time-delayed mite trap and integrated pest control method using the same according to the present invention, the time difference between the first trap and the second trap is one week (7 days), and the mite trap insertion and mite removal / extermination activities from step 1 to step 4, and the repetition thereof, are performed exactly every 7 days. This is very welcome for beekeepers during the busy season, that is, Step If step 1 is started on a Monday, then all subsequent steps can be performed on the same Monday. For beekeepers who handle dozens or hundreds of beehives (breeding boxes), the time-delayed mite trap and the comprehensive pest control method using the present invention can be performed on the same day of the week determined for each beehive, resulting in a particularly remarkable effect of simplifying the work and greatly reducing complexity. This effect can continue until around August, when the bee colony strength stabilizes, provided that the beekeeping situation is going well, which is a very favorable effect for beekeepers. Claim 3 This is a particularly noteworthy invention.

[0075] (Claim 4) Claim 4 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 4 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frameThis invention relates to a time-delay mite trap control method that uses a mite trap, and is characterized by the fact that mites, particularly Varroa mites, can be eradicated by raising honeybees in the following order from Step 1 to Step 5. Step 1. Claim 4 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame And, The aforementioned honeycomb, frame, or foundation frame Two frames of any size, or beeswax-made male beehive foundations or beeswax-made male beehive foundation frames, are attached to the bottom of the hive and one of them is designated as the first trap, and the other is designated as the second trap, and other inside the hive (beekeeping box) honeycomb, frame, or foundation frame Insert it at any position within the array. Step 2. In Step 1, male bee cells are created in the first and second traps, male bee eggs are laid by the queen bee, and when the larvae reach the second to fourth instar stage, The aforementioned honeycomb, frame, or foundation frame Remove the second trap, cut off and remove the male bee cells that were made in the second trap, and then the honeycomb, frame, or foundation frame It is then reinserted into its original position within the nest box. Step 3. During the pupal stage of male bee larvae in the male bee cell created in the first trap, The aforementioned honeycomb, frame, or foundation frame Remove the first trap, cut off and remove the male bee cells that were made in the first trap, and then the honeycomb, frame, or foundation frame It is then reinserted into its original position within the nest box. Step 4. During the pupal stage of male bee larvae in the male bee cells created in the second trap, The aforementioned honeycomb, frame, or foundation frame Remove the second trap, cut off and remove the male bee cells that were made in the second trap, and then the honeycomb, frame, or foundation frame It is then reinserted into its original position within the nest box. Step 5. Steps 3 and 4 described above are repeated.

[0076] Claim 4 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 4 This invention relates to a time-delay mite trap and an integrated pest control method using the same, wherein one of two frames of any size, beeswax-made male beehive foundations, or beeswax-made male beehive foundation frames, which are attached to the lower part of the time-delay mite trap, frame, or foundation frame, is designated as the first trap and the other as the second trap. (See Figure 2: described later) In (I will explain the details.)

[0077] In other words, the depth dimension of the upper comb, frame, or foundation frame is two-thirds to one-third of the depth dimension of the inside of the beehive, and two frames, beeswax-made male bee foundations, or beeswax-made male bee foundation frames are connected below it. Specifically, this is a time-delay mite trap and an integrated pest control method using it, in which one of the two connected frames, beeswax-made male bee foundations, or beeswax-made male bee foundation frames at the bottom is the first trap and the other is the second trap.

[0078] Claim 4 As described in Step 1, the comb, frame, or foundation frame used in the time-delay mite trap and the integrated pest control method using the same according to the present invention consists of only one piece in total, so it can be inserted at any position in the beehive. Preferably, it can be inserted in the central part where mites are easily collected. If foundation is provided, the upper foundation (part a in Figure 2) must be for female bees, and the two trap foundations connected at the bottom (parts b and c in Figure 2) must be for male bees. The size of the honeycomb hexagon of the female bee foundation is smaller than the size of the honeycomb hexagon of the two male bee foundations at the bottom. Claim 1 A container containing a mite-attracting compound (part d in Figure 2) is preferable because it can strongly attract and collect mites.

[0079] After Step 1, male bee cells are built in the first and second traps connected to the bottom, and the queen bee lays male bee eggs, raising the larvae. When the male bee larvae reach the second to fourth instar stage, in Step 2, the comb, frame, or foundation frame is removed, and all male bee cells built only in the second trap are cut out and removed. The first trap is left as is and reinserted into its original position in the hive. After Step 2, the male larvae in the first trap enter the capping stage and begin to attract mites. In the second trap, male bee cells are built again from the beginning, marking the start of one cycle for the second trap as described earlier, and this is where the half-cycle time difference between the first and second traps arises.

[0080] In step 3, during the pupal stage of the male bee larvae in the male bee cells created in the first trap, the comb, frame, or foundation frame is removed, the male bee cells created in the first trap are cut off and disposed of, and then the comb, frame, or foundation frame is reinserted into its original position in the hive. At the same time, the second trap enters the stage of capping the male larvae and begins to function as a mite attractant.

[0081] In step 4, during the pupal stage of the male bee larvae created in the second trap, remove the comb, frame, or foundation frame, cut out and dispose of the male bee cells created in the second trap, and then reinsert the comb, frame, or foundation frame back into its original position in the hive. Then, repeat steps 3 and 4 to successively attract, remove, and eliminate mites in the hive.

[0082] In other words, Claim 4 The time-delayed mite trap and the integrated pest control method using the same according to the present invention, as described herein, have the great effect of being able to create both a first trap and a second trap at the bottom of just one honeycomb board, frame, or foundation frame, and attracting and removing mites by providing a time difference between them. Claim 1 If you have the mite-attracting compound container described above, the mite-attracting effect will be outstanding.

[0083] (Claim 5) Claim 5 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 5 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame This invention relates to a time-delay mite trap control method that uses a mite trap, and is characterized by the fact that mites, particularly Varroa mites, can be eradicated by raising honeybees in the following order from Step 1 to Step 5. Step 1. Claim 5 The time-delay mite trap and the integrated pest control method using it described herein honeycomb, frame, or foundation frame And, The aforementioned honeycomb, frame, or foundation frame A single frame of any size, or a beeswax male beehive foundation or a beeswax male beehive foundation frame, attached to the lower part thereof, is designated as the first trap. honeycomb, frame, or foundation frame The space below the first trap, excluding the first trap, is designated as the second trap, and the other parts of the beehive (honeybee rearing box) honeycomb, frame, or foundation frame Insert it at any position within the array. Step 2. In the first trap mentioned in Step 1, a male bee cell is created, male bee eggs are laid by the queen bee, and when the larvae reach the second to fourth instar stage, The aforementioned honeycomb and / or frames or foundation frames Remove the second trap, cut off and remove the male bee cells that were made in the second trap, and then the honeycomb, frame, or foundation frame It is then reinserted into its original position within the nest box. Step 3. During the pupal stage of male bee larvae in the male bee cell created in the first trap, The aforementioned honeycomb, frame, or foundation frame Remove the first trap, cut off and remove the male bee cells that were made in the first trap, and then the honeycomb, frame, or foundation frame It is then reinserted into its original position within the nest box. Step 4. During the pupal stage of male bee larvae in the male bee cells created in the second trap, The aforementioned honeycomb, frame, or foundation frameRemove the second trap, cut off and remove the male bee cells that were made in the second trap, and then the honeycomb, frame, or foundation frame It is then reinserted into its original position within the nest box. Step 5. Steps 3 and 4 described above are repeated.

[0084] Claim 5 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 5 The time-delay mite trap and the integrated pest control method using the same described herein use a honeycomb, frame, or foundation frame of any size, which is attached to the lower part of the honeycomb, frame, or foundation frame. This time-delay mite trap and the integrated pest control method using the same use a honeycomb, frame, or foundation frame of any size, which is attached to the lower part of the honeycomb, frame, or foundation frame. This attached frame or beeswax foundation or beeswax foundation frame becomes the first trap, and the remaining space becomes the second trap.

[0085] In other words, the depth of the upper comb, frame, or foundation frame is two-thirds to one-third of the depth of the inside of the beehive, and a single frame, beeswax-based male bee foundation, or beeswax-based male bee foundation frame is attached to the lower part. Claim 4 Similarly, this is a time-delay mite trap that uses a comb, frame, or foundation frame as a single mite trap comb, frame, or foundation frame, and an integrated pest control method using it.

[0086] Claim 5 As described in Step 1, the time-delay mite trap according to the present invention and the comb, frame, or foundation frame used in the integrated pest control method using the same are Claim 4 Since there is only one piece, it can be inserted at any position in the hive. Preferably, it can be inserted in the central part where mites are easily attracted. Similarly, when providing foundations, the upper foundation should be for female bees, and the single trap foundation at the bottom should be for male bees. Claim 4 Similarly, Claim 1A container containing a mite-attracting compound is preferable because it can strongly attract and collect mites.

[0087] After Step 1, male bee cells are created in the first trap, which is connected to the bottom. Similarly, male bee cells are also created in the empty space of the second trap, although this tends to be delayed compared to the first trap. The queen bee lays male bee eggs, and the larvae are raised. When the male bee larvae in the first trap reach the second to fourth instar, in Step 2, the comb, frame, or foundation frame is removed, and all the male bee cells created only in the empty space of the second trap are cut out and removed. The first trap is left as is and reinserted into its original position in the hive. After Step 2, the male larvae in the first trap enter the capping stage and begin to attract mites. In the empty space of the second trap, male bee cells begin to be created again from the beginning, marking the start of one cycle in the second trap, and this is where the half-cycle time difference between the first and second traps arises.

[0088] In step 3, during the pupal stage of the male bee larvae in the male bee cells created in the first trap, the comb, frame, or foundation frame is removed, the male bee cells created in the first trap are cut out and disposed of, and then the comb, frame, or foundation frame is reinserted into its original position in the hive. Subsequently, the male larval cells created in the empty space of the second trap enter the lidding stage and begin to function as a mite attractant.

[0089] In step 4, during the pupal stage of the male bee larvae created in the second trap, remove the comb, frame, or foundation frame, cut out and dispose of the male bee cells created in the second trap, and then reinsert the comb, frame, or foundation frame back into its original position in the hive. Then, repeat steps 3 and 4 to successively attract, remove, and eliminate mites in the hive.

[0090] In other words, Claim 5The time-delay mite trap and integrated pest control method using the same according to the present invention, as described above, creates a first trap and a second trap within a single comb frame or foundation frame, but the second trap utilizes a space that does not require connecting comb frames or beeswax drone bee foundation frames. In other words, it has the effect of reducing the cost and connection work of one comb frame or beeswax drone bee foundation frame. For beekeepers, the reduction in work is a very welcome effect. Claim 5 The invention described can exert its remarkable effects.

[0091] (Claim 6) Claim 6 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 2, or Claim 3, or Claim 4, or Claim 5 This invention relates to a time-delay mite trap and a comprehensive pest control method using it, which involves repeating the steps of each time-delay mite trap comprehensive pest control method described above, in addition to the steps 1 to 3 below. Step 1. The queen bee in the beehive (honeybee rearing box) is isolated in a queen excluder or queen cage to stop egg-laying in the cells for approximately two to three weeks, or the entire beehive is moved to a cool environment to stop the queen bee from laying eggs in the cells for approximately two to three weeks. Step 2. When the remaining mite traps develop into capped larvae (male bee pupae with caps on their cells), the capped larvae are cut off and removed along with the mites. Step 3. Approximately two to three weeks after Step 1, a mite control agent (registered trademark: Apistan, Apivar, or Timovar, etc.) is used for a short period of time according to the instructions to eliminate any mites that remain and parasitize worker bees.

[0092] Claim 6 The time-delay type mite trap and the comprehensive pest control method using the same, as described in the present invention, Claim 2, or Claim 3, or Claim 4, or Claim 5This method is characterized by being implemented after repeating the steps of the comprehensive pest control method using each time-delay mite trap described above. Even if the comprehensive pest control method using each time-delay mite trap is repeated for several weeks to several months, it is difficult to completely eradicate mites that parasitize worker bees (female bees). Furthermore, as long as mites parasitizing female bees remain, there is an inherent risk that mite reproduction will increase again in the hive after the completion of the comprehensive pest control method using time-delay mite traps.

[0093] Furthermore, from the end of July to the beginning of August, as the queen bee's egg-laying peaks, the production of male bees also decreases. This reduces the number of male bee larva cells, and mites, seeking a place to live, rapidly invade worker bee larva cells. If left unchecked, this surge in mites can lead to the collapse of the bee colony.

[0094] Claim 6 The time-delay mite trap and integrated pest control method using the same, as described in this invention, is a method of controlling remaining mites after the number of mites in the beehive has decreased to a minimum by using a control method with a time-delay mite trap, and is highly effective in minimizing the number of mites in the beehive. Furthermore, because it is a method of controlling a small amount of remaining mites, the amount of mite control agents (registered trademarks: Apistan, Apivar, or Timovar, etc.) used can be kept to a minimum. Therefore, it has the advantage of reducing the cost and number of times mite control agents are used, while also having a very high mite control effect. In addition, it also leads to an improvement in the quality of the honey collected.

[0095] In Step 1, the queen bee is isolated or placed in a cool, air-conditioned environment for two to three weeks to stop egg-laying and eliminate bee larvae from the cells, thereby eliminating all larvae and parasitic mites. Mites remain in the covered cells within the trap along with male bee pupae, so these are also completely removed and eradicated, leaving only adult worker bees that may still have mites, before applying the mite control agent. In other words, the mite control agent is targeted at worker bees, and because worker bees have high resistance to mite control agents, the adverse effects of the mite control agent on the bee colony and the collected honey can be minimized.

[0096] Incidentally, the method described above of isolating the queen bee to stop egg-laying is called the queen bee isolation method. It is also called the queen excluder method, and a queen excluder is a container with a gap that only worker bees can pass through, preventing the larger queen bee from entering.

[0097] Claim 6 The time-delay mite trap and integrated pest management method using the same, as described in the present invention, embody the concept of Integrated Pest Management (IPM), represent the ultimate act of invention as a method for solving the problems of the present invention, and aim for the ultimate eradication of mites. Claims 2 to 5 After controlling mites using one of the methods described above, Claim 6 The present invention, which aims to further eliminate residual mites by the method described herein, and the integrated pest control method using the same, can be said to be an invention that encompasses all methods for eliminating the mite habitat.

[0098] After the queen bee is released from the queen excluder, she immediately begins laying eggs within the hive colony. Egg-laying and brood rearing during this period lead to the production of a colony for overwintering (winter bees: bees whose bodies are prepared for overwintering pupation, also known as winter bees). Therefore, controlling and eliminating mites greatly contributes to securing healthy bees suitable for overwintering.

[0099] It goes without saying that the timing of implementing the integrated pest management (IPM) method, which combines the above-described time-delay mite trapping method with queen bee isolation in a queen excluder and the use of mite control agents, must be adjusted according to the seasonal environment of the region where beekeeping is conducted. It is clear that the present invention can be maximized by coordinating the time-delay mite trapping method, queen bee isolation in a queen excluder, and the use of mite control agents as an integrated mite pest management (IPM) method, timing everything to coincide with the ecological behavior of honeybees, such as the start of activity, peak season, resting period, winter bee production period, and overwintering period.

[0100] The above describes the time-delay type mite trap and the comprehensive pest control method using the same according to the present invention, as a means to solve the above problems, from claim 1 to Claim 6 Although the above has been explained, the present invention is not limited to the embodiments described above, and can be implemented with various modifications without departing from the spirit of the invention. All suitable forms for different applications are included in the technical concept of the present invention. Furthermore, we assert that all equivalent methods using prior art are also included in the technical concept of the present invention. [Effects of the Invention]

[0101] In other words, according to the present invention, it is possible to develop a beekeeping technique that effectively controls and eradicates Varroa mites in the beekeeping industry in a simple and easy-to-use manner, without requiring the beekeeper's labor or the cost of installing mechanical equipment, and minimizing the use of chemical substances such as pesticides, thereby producing pure honey with minimal impurities and chemical contamination. The present invention is a mite control method that makes maximum use of the natural ecology and habits of bees and mites, and can minimize adverse impacts on the environment. In addition, it can also provide a solution to the above problems from the perspective of Integrated Pest Management (IPM), which minimizes the use of pesticides. [Brief explanation of the drawing]

[0102] [Figure 1] This is a perspective conceptual diagram showing the overall image of a typical beehive (beekeeping box). [Figure 2] This is a conceptual diagram showing an overall image of a honeycomb, honeycomb frame, or honeycomb foundation frame according to one embodiment of the invention described in claim 1 of the present invention. [Figure 3] This is a conceptual diagram showing each step according to one embodiment of the invention described in claim 3 of the present invention. [Figure 4] This is a conceptual diagram showing the overall timeline of each step according to one embodiment of the invention described in claim 3 of the present invention. [Modes for carrying out the invention]

[0103] Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings. In the following, the scope necessary for explaining how to achieve the objectives of the present invention will be schematically shown, and the scope necessary for explaining the relevant parts of the present invention will be mainly explained, with any parts that are omitted from the explanation being based on prior art.

[0104] Figure 1 is a perspective conceptual diagram showing the overall image of a typical beehive (beekeeping box). As shown in the figure, the comb, frame, or foundation frame inserted into the beehive 1 generally consists of comb 2, frame 3, and foundation 4. Comb 2 is sometimes referred to as comb 2 including the frame 3, foundation 4, and the bee cells that have been created. Frame 3 is a frame of boards that covers the outer perimeter. Foundation 4 is a thin beeswax plate with a honeycomb-like (hexagonal) pattern imprinted on it to make it easier for bees to build cells; it can also be made of plastic. The honeycomb size of foundation 4 ranges from small ones for female bee larvae to slightly larger ones for male bee larvae. Frame 3 on which foundation 4 is installed is called a foundation frame.

[0105] As shown in Figure 1, generally, the comb 2, comb frame 3, or comb foundation frame is designed so that approximately 10 pieces fit within the internal depth dimension 5 of the hive 1, the internal width dimension 6 of the hive 1, and the internal length dimension 7 of the hive 1. The entrance 9 is the entry and exit point for the bees, and the inside of the hive is sealed except for the entrance 9.

[0106] In one embodiment of the invention described in claim 1 of the present invention, the comb 2, comb frame 3, or comb foundation frame shown in Figure 1 has a depth dimension that is approximately two-thirds to one-third of the internal depth dimension of the hive. Furthermore, when a comb foundation 3 is used, it is a comb foundation for worker bees (female bees). This comb foundation for female bees may be made of beeswax or plastic. The purpose is to encourage female bees to build female bee cells in the comb foundation, creating a state where male bee cells are scarce, and thereby encouraging the bees to actively build male bee cells in the space below.

[0107] Normally, bee colonies can build male cells anywhere on the comb, frame, or foundation within the hive. However, if a comb, frame, or foundation is provided that is designed to induce the bees to build male cells in a specific location, they tend to prefer building them there and refrain from building them elsewhere. The mite trap according to the present invention utilizes this behavior.

[0108] Figure 2 shows the present invention. Claim 1 This is a conceptual diagram showing the overall image of a honeycomb, frame, or foundation frame according to one embodiment of the invention described in claim 2. As shown in the figure, the honeycomb, frame, or foundation frame according to one embodiment of the invention described in claim 2 of the present invention has an upper part a that serves as a foundation for female bees, allowing female bees to build honeycomb cells. The present invention also includes cases where there is no foundation, but basically female bees are allowed to build honeycomb cells in the upper part a. The foundation for female bees in part a may be made of beeswax or plastic.

[0109] As shown in Figure 2, parts b and c are Claim 1 The frame is of any size as described above, or a beeswax male bee foundation or beeswax male bee foundation frame. In the case of Figure 2, two frames, b and c, are connected to a. However, b and c do not necessarily have to be connected. Although it is difficult to see in Figure 2, since a is a foundation for female worker bees, the honeycomb hexagon size is smaller than the honeycomb hexagon size of the male bee foundations in b and c. In the case of Figure 2, the time-delay mite trap and the integrated pest control method using the same according to the present invention are shown. Claim 4 It is used in the method described below.

[0110] Sections b and c in Figure 2 depict beeswax-based male beehive foundations, but of course, the present invention also includes cases where only the hive frame 3 is used, without beeswax-based male beehive foundations.

[0111] The present invention relates to a time-delay type mite trap and a comprehensive pest control method using the same. Claim 5The method of the invention described above shows only parts a and b in Figure 2, with part c being empty. Furthermore, the invention described in claim 1 of the time-delay type mite trap and the comprehensive pest control method using the same shows the case where part d in Figure 2 is absent and the case where part d in Figure 2 is added.

[0112] Section d in Figure 2 shows the time-delay mite trap and the integrated pest control method using the same according to the present invention. Claim 1 As an example, a container containing a mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation capping, is shown. The container d containing the mite-attracting compound only needs to have a structure that allows the mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters, to be released to the outside of the container.

[0113] Figure 3 shows the present invention. Claim 3 This is a conceptual diagram showing each step according to one embodiment of the invention of the method described above. It is a conceptual diagram showing a side view of the honeycomb inside the beehive. As shown in Figure 3, ten honeycomb frames 2, frames 3, or foundation frames are inserted inside the beehive 1, and the honeycomb frames, frames, or foundation frames having trap section A and trap section B are the honeycomb frames, frames, or foundation frames described in claim 1, respectively, with a depth dimension of two-thirds to one-third of the depth dimension inside the beehive. Sections A and B are the parts where worker bees create male bee cells and serve as mite traps.

[0114] In other words, Figure 3 is a diagram showing one embodiment of a time-delay mite trap control method in which a comb, frame, or foundation frame used in the time-delay mite trap and integrated pest control method using the same as described in claim 1 is used as a mite trap. Claim 3 This describes a time-delay mite trap and an integrated pest control method using it. As shown in Figure 3, the present invention Claim 3 Each step of an embodiment of the invention described below will be explained.

[0115] Step 1, As shown in Step 1 of Figure 3, the first trap comb A, which is a comb, frame, or foundation frame with the lower portion cut off, is inserted into the left outside of the beehive. (Hereafter, the comb, frame, or foundation frame having section A which becomes the first trap will be referred to as "first trap comb A," and similarly, the comb, frame, or foundation frame having section B which becomes the second trap will be referred to as "second trap comb B.") If the honeybee colony is at its peak, male cells will soon be formed in the cut-out space A, and at the same time, the queen bee will lay eggs that will develop into male bees. The queen bee can choose to lay male or female (worker) eggs depending on the size of the cells, etc. Step 2, One week later (week 2), insert another similarly prepared second trap honeycomb board B onto the opposite side of the outside. Step 3, Two weeks later (week 3), when the first trap comb A is removed, the male bee cells in section A have grown and the cells have been capped. These male bee cell sections are cut off and disposed of. In other words, the male bee cells that attracted and invaded in large numbers of mites are eliminated. Then, the first trap comb A, from which the male bee cells have been cut off, is reinserted in the same place. Step 4, Three weeks later (in the fourth week), in the second trap comb B, similarly, the capped male bee cells (cells containing male bee pupae with caps) that have formed at the bottom of the comb are cut off. In other words, the male bee cells that have attracted and invaded in large numbers of mites are eliminated. Then, in the same way, the second trap comb B is returned to its original location. Step 5. Repeat steps 3 and 4 above.

[0116] As a result, the mites are attracted to the male bee cells, which are constantly cut off, thus enabling continuous mite control. In other words, the first trap comb A and the second trap comb B described above are mite-attracting traps for mite control. This method makes it possible to keep mite traps constantly installed in the hive, effectively minimizing mite damage.

[0117] As shown above with reference to Figure 3, the time-delay type mite trap extermination method according to the present invention Claim 3 The invention of the method described above involves a one-week period between inserting the first trap comb and inserting the next, followed by another one-week period for cutting off and removing the trap portion of the first trap comb and reinserting it, and then another one-week period for cutting off and removing the trap portion of the next trap comb and reinserting it, and so on, repeating the work in one-week increments. This was devised because the period for creating male cells and the period for raising male bee larvae can be conveniently adjusted in one-week increments from the start of spring activity to the peak of the bee colony's activity. However, Claim 3 It should be noted that in other time-delay mite trapping methods according to the present invention, this one-week unit is not limited. The length of this work unit can be adjusted according to the growth stage of the honeybees, as is the case with this invention.

[0118] The unit period for the above repetitive work will vary depending on various factors such as the number and activity level of the bee colony, seasonal changes, whether it is a warm or cold region, and the activity level of the queen bee. In other words, the period for the above repetitive work in the time-delayed mite trap extermination method according to the present invention is Claim 3 Except for the exceptions mentioned above, this is not limited to one week, and can be adjusted depending on the bee colony and beekeeping conditions at the time. Conversely, it should be noted that this period should be adjusted considering various factors.

[0119] Incidentally, when using plastic male bee foundations for mite trap combs, the post-trap processing is not only difficult to cut off, but also requires cleaning with a high-pressure washer, making it a very time-consuming process. For example, inserting empty foundations (e.g., wire frames) to allow male bees to build comb cells is a simpler process, as it only requires cutting off the comb made of beeswax. In addition, the present invention Claim 1 If you use the foundation comb described above, it is made from beeswax and can be similarly cut off, and since it is provided for male bees, it has the advantage that worker bees can quickly and reliably build male cells that serve as mite traps.

[0120] In August, after the queen bee's peak activity period, the bee colony may decrease its rate of building male cells on the trap comb and may even begin building worker bee cells at the bottom of the trap comb. In such cases, the number of trap combs may be reduced from two (A and B) to one. Conversely, during the queen bee's peak activity period and the peak of the bee colony's activity, the number of trap combs may be increased, for example, to two of each (A and B).

[0121] Figure 4 shows the present invention. Claim 3 This is a conceptual diagram showing the overall schedule of each step according to one embodiment of the invention of the method described above. In other words, it simulates a method of regularly performing a time-delayed mite extermination method using mite traps. As shown in the figure, the upper section of the figure shows the schedule and days of the week, for example, from April, which is the peak season for honeybees. The middle section shows the present invention. Claim 3 The diagram below shows the growth cycle from trap insertion to egg-laying, hatching, and pupation of male honeybees in the first trap used in the time-delay mite trap extermination method described above. Similarly, the diagram below shows the present invention Claim 3 This shows the growth cycle from trap insertion to egg-laying, hatching, and pupation of male honeybees in the second trap used in the time-delay mite trap extermination method described above.

[0122] As shown in Figure 4, the egg-laying, hatching, and pupation cycle of male bees in the first trap and the egg-laying, hatching, and pupation cycle of male bees in the second trap are repeated with a time difference of 7 days.

[0123] The section labeled "Trap" in Figure 4 indicates the period when male bee larvae in male bee cells, which are set up as mite traps, are about to pupate. During this time, they release a large amount of capping pheromones, attracting mites (mainly female mites) into the male bee larval cells. Because the first and second traps repeat the growth cycle of the male honeybee larvae in a time-delayed manner, the period when the larval capping pheromones that attract mites are released is repeated almost continuously within the hive. Therefore, it is possible to continuously attract and eliminate mites within the hive.

[0124] To reiterate, the "male bee larva capping pheromone" mentioned above refers to the present invention. Claim 1 This refers to the mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation.

[0125] As shown in Figure 4, performing mite trap removal using a time-delay system allows mite trap insertion and mite removal to be carried out regularly and continuously on the same day of the week without being forgotten, making it a very simple and convenient operation for beekeepers. Furthermore, if a simple comb, frame, or foundation (foundation frame) such as the lower cut comb described in claim 1 is used as a mite trap, it becomes an inexpensive mite trap, which is very economically beneficial. In addition, the process of cutting out the male bee larvae cells that have formed in the lower space of the cut comb for mite traps involves cutting out the honeycomb including the beeswax cells, so it can be easily and simply cut and removed with a utility knife or similar tool, making the work simple and very convenient.

[0126] During the period in which this time-delayed mite trapping method is continued, mite infestations are minor, eliminating the need for pesticides and resulting in significant cost savings. For large-scale beekeepers operating with dozens to hundreds of beehives, this is a simple, highly effective, and worthwhile mite trapping method.

[0127] During the peak of spring, some bee colonies may complete the entire comb frame with cells overnight and lay male eggs. In this case, the male bee larvae in the cells grow and pupate all at once, so the effective period as a mite trap—the time until all the cells are capped—is shortened to 2-3 days. However, while this can happen quickly, conversely, the period for creating male cells may be delayed, resulting in a wider range of development for the male larvae, and a longer capping period. In other words, the trapping period may be extended. In such cases, the timing of inserting the mite trap comb frame and the period of cutting off capped male bee brood cells, as described in the previously mentioned time-delayed mite trap control method, can be adjusted by anticipating such variations in the number of days or duration. Regardless of whether the above repetition period is short or long, the purpose of the time-delayed mite trap and the comprehensive pest management method using it according to the present invention remains the same, and these responses are encompassed within the technical concept of the present invention.

[0128] Furthermore, in beekeeping in warm regions, queen bees may not stop laying eggs even in December, for example. In this case, bee larvae are also present, and mite reproduction continues. Therefore, if the queen bee is isolated during such a period to prevent egg-laying from stopping, the time-delay mite trapping method according to the present invention is extremely effective for mite control, taking into account the above-mentioned adjustment of the repetition period.

[0129] The present invention is not limited to the embodiments described above, and can be implemented with various modifications without departing from the spirit of the invention. However, all such embodiments are included in the technical concept of the present invention.

[0130] All suitable forms for various applications similar to the present invention are included within the technical concept of this invention. Furthermore, we assert that all equivalent methods using prior art are also encompassed within the technical concept of this invention. [Industrial applicability]

[0131] As described above, the invention of this application provides and can be implemented in the beekeeping industry a beekeeping technique that effectively eradicates and controls Varroa mites comprehensively in a simple method that avoids complexity, requires little labor, does not incur costs or power for installing mechanical equipment, and minimizes the use of chemical substances such as pesticides, thereby enabling the collection of pure honey with minimal impurities and chemical contamination.

[0132] Furthermore, this invention is a mite control method that makes maximum use of the natural ecology and habits of honeybees and mites, and can minimize adverse environmental impacts. In addition, it can provide society with the concept of Integrated Pest Management (IPM), which minimizes the use of pesticides.

[0133] Furthermore, this invention is applicable not only to Western honeybees but also to beekeeping of all types of honeybees. Therefore, this invention will bring great benefits not only to the beekeeping industry but also to various industries that use honey as a raw material. [Explanation of Symbols]

[0134] 1. Beehive (honeybee keeping box) 2 nest board 3 Nest frame 4 Nest foundation 5 Depth dimensions 6. Width 7 Width dimensions 8 Length dimensions 9 Nest Gate 10. Honeycomb, honeycomb frame, honeycomb foundation (honeycomb foundation frame) according to the present invention 11. First trap nest A 12. Second trap board B 13. Mites (Varroa mites)

Claims

1. In beekeeping, comb frames, or foundation frames are inserted into the inside of a beehive. The aforementioned comb, frame, or foundation frame is characterized in that its depth is two-thirds to one-third of the depth of the inside of the beehive, the foundation is for female bees, and the lower part of the comb, frame, or foundation frame is designed to facilitate the construction of male bee cells by worker bees. and A comb, comb frame, or comb foundation frame characterized by having one or more beeswax male bee foundations or beeswax male bee foundation frames of any size connected to the lower part of the aforementioned comb, comb, comb frame, or comb foundation frame, in order to facilitate the creation of male bee cells. And, Furthermore, a container containing a mite-attracting compound, which is the odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation capping, is provided near the connection point between the honeycomb board or frame or foundation frame and the beeswax male bee foundation or beeswax male bee foundation frame of any size. A time-delay mite trap with the following characteristics, and a comb, frame, or foundation frame used in an integrated pest control method using the same.

2. In beekeeping, comb frames, or foundation frames are inserted into the inside of a beehive. The aforementioned comb, frame, or foundation frame is characterized in that its depth is two-thirds to one-third of the depth of the inside of the beehive, the foundation is for female bees, and the lower part of the comb, frame, or foundation frame is designed to facilitate the construction of male bee cells by worker bees. and A time-delay mite trap characterized by having one or more beeswax male beehive foundations or beeswax male beehive foundation frames of any size connected to the lower part of the aforementioned comb, comb frame, or foundation frame, in order to facilitate the construction of male bee cells, and a comb, comb frame, or foundation frame used in an integrated pest control method using the same, This is a time-delay mite trap control method that uses a mite trap, and by raising honeybees in the following order from Step 1 to Step 5, mites, especially Varroa mites, can be eradicated. A time-delay type mite trap with the following characteristics, and an integrated pest control method using the same. Step 1. One or more combs, frames, or foundation frames used in the time-delay mite trap and the integrated pest control method using it are inserted as the first trap on one side of the arrangement of other combs, frames, or foundation frames inside the beehive (honeybee rearing box). Step 2. In step 1, a male bee cell is created at the bottom of the first trap, male bee eggs are laid by the queen bee, and when the larvae reach the second to fourth instar stage, One or more combs, frames, or foundation frames used in the time-delay mite trap and the integrated pest control method using it are inserted as a second trap on the other side of the arrangement of other combs, frames, or foundation frames within the hive. Step 3. Step 1: During the pupal stage of the male bee larvae in the male bee cells formed at the bottom of the first trap, the first trap is removed, the male bee cells formed at the bottom of the first trap are cut off and disposed of, and then the first trap is inserted back into its original position. Step 4. Step 2: During the pupal stage of the male bee larvae in the male bee cells formed at the bottom of the second trap, the second trap is removed, the male bee cells formed at the bottom of the second trap are cut off and disposed of, and then the second trap is inserted back into its original position. Step 5. Steps 3 and 4 described above are repeated.

3. In beekeeping, comb frames, or foundation frames are inserted into the inside of a beehive. The aforementioned comb, frame, or foundation frame is characterized in that its depth is two-thirds to one-third of the depth of the inside of the beehive, the foundation is for female bees, and the lower part of the comb, frame, or foundation frame is designed to facilitate the construction of male bee cells by worker bees. and A time-delay mite trap characterized by having one or more beeswax male bee foundations or beeswax male bee foundation frames of any size connected to the lower part of the aforementioned comb, frame, or foundation frame to facilitate the construction of male bee cells, and a time-delay mite trap control method using a comb, frame, or foundation frame as a mite trap for integrated pest management, wherein mites, particularly Varroa mites, can be eradicated by raising honeybees in the following order from Step 1 to Step 5. A time-delay type mite trap with the following characteristics, and an integrated pest control method using the same. Step 1. One or more combs, frames, or foundation frames used in the time-delay mite trap and the integrated pest control method using it are inserted as the first trap on one side of the arrangement of other combs, frames, or foundation frames inside the beehive (honeybee rearing box). Step 2. Seven days after Step 1, One or more combs, frames, or foundation frames used in the time-delay mite trap and the integrated pest control method using it are inserted as a second trap on the other side of the arrangement of other combs, frames, or foundation frames within the hive. Step 3. Seven days after Step 2, The first trap is removed, the male bee cells formed at the bottom of the first trap are cut off and disposed of, and then the first trap is inserted back into its original position. Step 4. Seven days after step 3, The second trap is removed, the male bee cells formed at the bottom of the second trap are cut off and disposed of, and then the second trap is inserted back into its original position. Step 5. Seven days after step 4, Steps 3 and 4 described above are repeated every 7 days.

4. In beekeeping, comb frames, or foundation frames are inserted into the inside of a beehive. The aforementioned comb, frame, or foundation frame is characterized in that its depth is two-thirds to one-third of the depth of the inside of the beehive, the foundation is for female bees, and the lower part of the comb, frame, or foundation frame is designed to facilitate the construction of male bee cells by worker bees. and A time-delay mite trap and a comb, comb, or comb frame used in an integrated pest control method using the same, characterized in that two or more beeswax male bee comb foundations or beeswax male bee comb foundation frames of any size are connected to the lower part of the aforementioned comb, comb, or comb foundation frame to facilitate the creation of male bee cells. This is a time-delay mite trap control method that uses a mite trap, and by raising honeybees in the following order from Step 1 to Step 5, mites, especially Varroa mites, can be eradicated. A time-delay type mite trap with the following characteristics, and an integrated pest control method using the same. Step 1. The aforementioned time-delay mite trap and the comb, frame, or foundation frame used in the integrated pest control method using the same, One of two beeswax drone bee foundations or beeswax drone bee foundation frames of any size, which are attached to the lower part of the aforementioned comb, frame, or foundation frame, is designated as the first trap, and the other as the second trap, and is inserted at any position in the arrangement of other combs, frames, or foundation frames within the beehive (honeybee rearing box). Step 2. In Step 1, male bee cells are created in the first and second traps, male bee eggs are laid by the queen bee, and when the larvae reach the second to fourth instar stage, The comb, frame, or foundation frame is removed, the male bee cells created in the second trap are cut off and removed, and then the comb, frame, or foundation frame is reinserted into its original position inside the hive. Step 3. During the pupal stage of male bee larvae in the male beehive cells created in the first trap, The comb, frame, or foundation frame is removed, the male bee cells created in the first trap are cut off and removed, and then the comb, frame, or foundation frame is reinserted into its original position inside the hive. Step 4. During the pupal stage of male bee larvae in the male beehive cells created in the second trap, The comb, frame, or foundation frame is removed, the male bee cells created in the second trap are cut off and removed, and then the comb, frame, or foundation frame is reinserted into its original position inside the hive. Step 5. Steps 3 and 4 described above are repeated.

5. In beekeeping, comb frames, or foundation frames are inserted into the inside of a beehive. The aforementioned comb, frame, or foundation frame is characterized in that its depth is two-thirds to one-third of the depth of the inside of the beehive, the foundation is for female bees, and the lower part of the comb, frame, or foundation frame is designed to facilitate the construction of male bee cells by worker bees. and A time-delay mite trap characterized by having one or more beeswax male bee foundations or beeswax male bee foundation frames of any size connected to the lower part of the aforementioned comb, frame, or foundation frame to facilitate the construction of male bee cells, and a time-delay mite trap control method using a comb, frame, or foundation frame as a mite trap for integrated pest management, wherein mites, particularly Varroa mites, can be eradicated by raising honeybees in the following order from Step 1 to Step 5. A time-delay type mite trap with the following characteristics, and an integrated pest control method using the same. Step 1. The aforementioned time-delay mite trap and the comb, frame, or foundation frame used in the integrated pest control method using the same, A single beeswax drone bee foundation or beeswax drone bee foundation frame of any size, attached to the lower part of the aforementioned comb, frame, or foundation frame, is designated as the first trap, and the space below the aforementioned comb, frame, or foundation frame, excluding the first trap, is designated as the second trap, which is inserted at any position in the arrangement of other combs, frames, or foundation frames within the beehive (honeybee rearing box). Step 2. In the first trap mentioned in Step 1, male bee cells are created, male bee eggs are laid by the queen bee, and when the larvae reach the second to fourth instar stage, The comb, frame, or foundation frame is removed, the male bee cells created in the second trap are cut off and removed, and then the comb, frame, or foundation frame is reinserted into its original position inside the hive. Step 3. During the pupal stage of male bee larvae in the male beehive cells created in the first trap, The comb, frame, or foundation frame is removed, the male bee cells created in the first trap are cut off and removed, and then the comb, frame, or foundation frame is reinserted into its original position inside the hive. Step 4. During the pupal stage of male bee larvae in the male beehive cells created in the second trap, The comb, frame, or foundation frame is removed, the male bee cells created in the second trap are cut off and removed, and then the comb, frame, or foundation frame is reinserted into its original position inside the hive. Step 5. Steps 3 and 4 described above are repeated.

6. A time-delay mite trap and a comprehensive pest control method using the same, in which a time-delay mite trap is used in any of Claims 2 to 5, wherein the time-delay mite trap is used in the following steps A to C. Step A. The queen bee in the beehive (honeybee rearing box) is isolated in a queen excluder or queen cage to stop egg-laying in the comb for approximately two to three weeks, or the entire beehive is moved to a cool environment to stop the queen bee from laying eggs in the comb for approximately two to three weeks. Step B. When the remaining mite traps develop into capped larvae (male bee pupae with caps on their cells), the capped larvae are cut off and removed along with the mites. Step C. Approximately two to three weeks after Step 1, a mite control agent (registered trademark: Apistan, Apivar, or Timovar, etc.) is used for a short period of time according to the instructions to eliminate any mites that remain and parasitize worker bees.

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