Topical skin preparations

Abstract

To provide a novel active ingredient that can be blended in a skin external preparation, and is useful for a skin external preparation for improved rough skin, antipruritic use, analgesic use, or sensitive skin.SOLUTION: A nerve growth factor production inhibitor contains solvent extract of Rosa damascena as an active ingredient.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an active ingredient that has excellent biological safety and is incorporated into external skin preparations (quasi-drugs, cosmetics, etc.).

Background Art

[0002] In recent years, an increasing number of people experience subjective symptoms such as erythema and itching on the skin due to the excessive response of the in-vivo immune system to various environmental factors and stress. For this reason, active ingredients for external skin preparations for antipruritic, analgesic, or anti-stress use are in demand, but no active ingredient that exhibits sufficient effectiveness and has excellent biological safety has been found yet.

Disclosure of the Invention

Problems to be Solved by the Invention

[0003] As a result of intensive studies aimed at solving the above problems, the present inventors found that an extract of Damask rose flowers exerts an effect of significantly suppressing the production of Nerve Growth Factor (NGF), which is a cause of hyperesthesia in the epidermis or dermis. Conventionally, it has been known from the techniques described in, for example, cited documents 1 to 2 that extracts of Damask rose flowers have an antioxidant effect, a whitening effect, or a skin turnover improving effect, but it was not known that extracts of Damask rose flowers exert an effect of suppressing the production of nerve growth factor.

[0004]

Patent Document 1

Patent Document 2

Means for Solving the Problems

[0005] The present invention is a nerve growth factor production inhibitor comprising, as an active ingredient, a solvent extract of Damask rose belonging to the genus Rosa of the family Rosaceae.

Effects of the Invention

[0006] According to the present invention, a topical skin preparation for improving rough skin, relieving itching, relieving pain, or for sensitive skin can be provided based on a component derived from natural products, which has excellent biocompatibility and inhibits the production of nerve growth factor (NGF). Furthermore, a topical skin preparation for improving itchy skin diseases (such as atopic dermatitis) can also be provided. [Brief explanation of the drawing]

[0007] [Figure 1] Figure 1 shows the results of a test evaluating the effect of inhibiting nerve growth factor production. [Modes for carrying out the invention]

[0008] Preferred embodiments of the present invention will be described in detail below. The extract material used in this invention is Rosa damascena, a member of the Rosaceae family, and any variety (including hybrids and subspecies) of Rosa damascena may be used.

[0009] The materials used in this invention may include the whole plant, leaves, flowers, stems, seeds, fruits, roots, etc., of the Damask rose, but the use of the whole plant or flowers is preferred.

[0010] To prepare the extract, first, the Damask rose (e.g., whole plant, flowers, etc.) is washed with water if necessary to remove foreign matter, then either left as is or dried, and finely chopped or pulverized as needed, and then brought into contact with the extraction solvent for extraction. Extraction can be carried out by contacting the extraction solvent according to conventional methods such as immersion, but supercritical fluid extraction can also be used in addition to immersion.

[0011] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform. These can be used individually or in combination of two or more.

[0012] Among these extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention, from the viewpoint of skin irritation and effectiveness, as well as their broad applicability to cosmetics. Preferred examples of using these hydrophilic solvents include, for example, the use of water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or a mixed solvent of water and lower alcohols (especially ethanol), or a mixed solvent of water and polyhydric alcohols (especially 1,3-butylene glycol, glycerin), but among these, water alone or a mixed solvent of water and 1,3-butylene glycol are particularly preferred.

[0013] When using a mixed solvent, the mixing ratio is preferably in the range of 1:10 to 20:1 by volume (hereinafter the same) for a mixed solvent of water and 1,3-butylene glycol, 1:10 to 25:1 for a mixed solvent of water and ethanol, and 1:10 to 20:1 for a mixed solvent of water and glycerin.

[0014] Furthermore, the weight ratio of the dried part of the Damask rose (e.g., the whole plant or flowers) to the extraction solvent is preferably in the range of 1:1 to 1:50, and more preferably in the range of 1:5 to 1:35.

[0015] While there are no particular limitations on the pH of the extract during preparation, it is generally preferable to have a pH in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be adjusted to the desired pH by adding an alkaline adjusting agent such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid.

[0016] Extraction conditions such as extraction temperature and extraction time vary depending on the type of solvent and pH used. For example, when using water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 0°C to 20°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0017] Furthermore, prior to or in parallel with the extraction process of the present invention, the Damask rose may be subjected to hydrolysis as needed. This may improve the storage stability of the Damask rose extract and allow for more effective use of the extract as a cosmetic ingredient.

[0018] When the extract is subjected to enzymatic hydrolysis, one or more enzymes selected from any of the following groups may be used: proteolytic enzymes such as actinase, papain, and pepsin; starch-degrading enzymes such as glucoamylase, α-amylase, and β-amylase; fibrinolytic enzymes such as cellulase, hemicellulase, and pectinase; and lipases. However, it is more preferable to use a combination of one or more enzymes selected from each of these groups.

[0019] The amount of enzyme added is preferably in the range of 0.01 to 10% by weight in total, and more preferably in the range of 0.1 to 2.0% by weight, relative to the solid content, for example, the whole plant or flower part of the Damask rose.

[0020] The extract prepared as described above may generally be adjusted to a pH of 3 to 8, and used as it is as a cosmetic compounding ingredient, or may be used after being concentrated to a desired concentration by vacuum concentration or the like. Further, the extract may be made into a dried product by a conventional method such as a spray drying method.

[0021] Further, the extract prepared as described above may be refrigerated for a certain period of time to enhance storage stability and the like, and then the supernatant may be used as a cosmetic compounding ingredient.

[0022] The extract of the present invention obtained as described above contains various active ingredients. For example, it was confirmed by measurement using liquid chromatography (manufactured by Hitachi High-Technologies Corporation; LaChrom ELITE) that the extract contains gallic acid, chlorogenic acid, and caffeic acid as organic acids.

[0023] Examples of uses of the external skin preparation containing the Damask rose extract of the present invention include cosmetics (including quasi-drugs), for example, basic cosmetics such as milky lotions, creams, lotions, essences, and packs; makeup cosmetics such as lipsticks, foundations, liquid foundations, makeup pressed powders, blushes, and face powders; cleansing cosmetics such as facial cleansers, body shampoos, hair shampoos, and soaps; hair restorers, and further bath additives and the like, but are of course not limited thereto.

[0024] When the Damask rose extract of the present invention is used in cosmetics, the blending amount of the Damask rose extract, as the solid content of the extract, is generally in the range of 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight for basic cosmetics; generally in the range of 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight for makeup cosmetics; and generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight for cleansing cosmetics.

[0025] When using the Damask rose extract of the present invention in cosmetics, in addition to the essential ingredient, the Damask rose extract, other ingredients commonly used in cosmetics, such as oily components, surfactants (synthetic and natural), humectants, thickeners, emulsifiers or emulsifying aids, preservatives and bactericides, powder components, UV absorbers, antioxidants, pigments, fragrances, and other physiologically active ingredients, may be used in combination as needed. Furthermore, there is no problem in combining the Damask rose extract of the present invention with other physiologically active ingredients, as long as the effectiveness and characteristics of the Damask rose extract are not impaired.

[0026] Here, oily components include, for example, lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cocoa oil, meadowfoam oil, bergamot oil, rosehip oil, coffee arabic seed oil, lavender oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, vanilla oil, plant-derived oils such as squalane; animal-derived oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; and liquid paraffin. Examples include hydrocarbons such as petrolatum, paraffin wax, and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, cetyl isooctanoate, 2-ethylhexylglyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.).

[0027] Furthermore, examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyethylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, and α-sulfonated fatty acid alkyl ethers. Anionic surfactants such as telates and polyoxyethylene alkylphenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammonium betaine, N,N,N-trialkyl-N-alkyleneammonium carboxybetaine, and N-acylamidopropyl-N′,N′-dimethyl-N′-β-hydroxypropylammonium sulfobetaine can be used.

[0028] Furthermore, emulsifiers or emulsifying aids may include stevia derivatives such as enzyme-treated stevia, saponins or their derivatives, casein or its salts (such as sodium), sugar-protein complexes, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, complexes of soybean-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (such as enzyme-treated stevia), silicates (such as aluminum and magnesium), carbonates (such as calcium and sodium), saponins and their derivatives, lecithin and its derivatives (such as hydrogenated lecithin), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (such as wheat, beans, and other grains).

[0029] Furthermore, examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, 2-methacryloyloxyethyl phosphorylcholine / butyl methacrylate copolymer solution, and sugars such as trehalose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), tuberose polysaccharide, elastin and its derivatives, collagen and its derivatives, NMF-related substances, hydrolyzed conchiolin, hydrolyzed silk, Sphingomonas culture, sphingoglycolipids, ceramides (human-type ceramides, yuzu ceramides, etc.), lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, Bletilla striata root extract, various amino acids and their derivatives.

[0030] Furthermore, examples of thickening agents include components derived from brown algae, green algae, or red algae such as alginic acid, agar, carrageenan, and fucoidan; Bletilla striata root extract; polysaccharides such as pectin, locust bean gum, aloe polysaccharide, and Alcaligenes-producing polysaccharide; gums such as xanthan gum, tragacanth gum, roasted bean gum, and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, mastic resin, and acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; and polyglutamic acid and its derivatives.

[0031] Furthermore, preservatives and disinfectants include, for example, urea; parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodinylurea), polyphosphate, propanediol, 1,2-pentanediol, various essential oils, bark distillates, radish ferment filtrate, and plant-derived ethanol or 1,3-butylene glycol from sugarcane, etc.

[0032] In addition, powder components include, for example, sericite, titanium dioxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powders, grain powders (rice, wheat, corn, millet, etc.), and legume powders (soybeans, adzuki beans, etc.).

[0033] Examples of UV absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0034] Examples of defoaming agents include ethanol, isopropanol, disiloxane, dimethylpolycyclosane, dimethicone silica, trisiloxane, silylated silica, dimethicone, trimethylsiloxysilicate, and DPG isobornyl ether.

[0035] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, astaxanthin, extract of Callicarpa japonica, extract of Bletilla striata root, extract of Paeonia lactiflora, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate, etc.).

[0036] Examples of chelating agents include trisodium ethylenediamine hydroxyethyl triacetate, EDTA or its salts, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, and tetrasodium hydroxyethanediphosphonate.

[0037] Examples of pH adjusting agents include citric acid or its salts, lactic acid or its salts, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide, and the like.

[0038] Furthermore, examples of whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, potassium 4-methoxysalicylate, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, and AMP (adenosine monophosphate, adenosine monophosphate). These may be used individually or in combination.

[0039] Furthermore, the above-mentioned kojic acid derivatives include, for example, kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Ascorbic acid derivatives include, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid-2-sulfate magnesium. Ascorbic acid sugar derivatives such as ester salts, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups include hexanoyl, octanoyl, and decanoyl groups), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc. Examples of hydroquinone derivatives include tetra-rubic acid fatty acid esters, 3-O-ethyl ascorbic acid, sodium L-ascorbic acid-2-phosphate-6-O-palmitate, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc., and as hydroquinone derivatives, arbutin (hydroquinone-β-D-glucopyrano Examples of tranexamic acid derivatives include α-arbutin (hydroquinone-α-D-glucopyranoside), tranexamic acid esters (e.g., lauryl tranexamic acid ester, hexadecyl tranexamic acid ester, cetyl tranexamic acid ester or their salts), amide forms of tranexamic acid (e.g., methyl tranexamic acid amide), resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, and 2,5-dihydroxybenzoic acid derivatives include, for example, 2,Examples of nicotinic acid derivatives include 5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate. Examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0040] Furthermore, the physiologically active ingredients include, for example, placenta extract, mulberry bark extract, saxifrage extract, perilla extract, mustard extract or its hydrolysate, fermented mustard extract, peony extract or its hydrolysate, lactic acid bacteria extract, bifidobacteria extract, yeast extract, beautyberry extract, lotus seed extract or its hydrolysate, lotus seed ferment, Job's tears hydrolysate, Job's tears seed ferment, royal jelly ferment, sake lees extract or its contents Ceramide, sake lees ferment, Pandanus amaryllis extract, Arcangelicia flava extract, Calendula extract, Chamomile extract, Coral grass extract, Rice leaf extract or hydrolysate thereof, Eggplant (Lotus, Long Eggplant, Kamo Eggplant, American Eggplant, etc.) extract or hydrolysate thereof, Apricot fruit extract, Seaweed extract such as Eggplant rotundifolia, Marine flowering plant extract such as Eelgrass, Soy milk ferment, Jellyfish water, Rice ferment extract, Rice extract or so Hydrolyzed product of, rice bran extract or its hydrolyzed product, germinated rice extract or its hydrolyzed product, germinated rice fermented product, black bean extract or its hydrolyzed product, brown sugar extract or its fermented product, ylang-ylang flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (e.g., liposomal linoleic acid), collagen and its derivatives derived from animals or fish, elastin and its derivatives, glycyrrhizic acid and its derivatives (e.g., dipotassium salt), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or its fermented product, camellia extract, red ginseng extract, kelp extract, loofah extract, sea lettuce extract, peach extract, plum extract, peach kernel extract, kiwi extract, sunflower extract, juazeiro (Zizyphus)Examples include joazeiro extract, pau d'arco bark extract, daylily extract or ferment, hibiscus flower extract or ferment, rhododendron extract, cherimoya extract, mango extract, mangosteen extract, sea lettuce extract, oolong tea extract, red bell pepper extract, orchid extract, sweet potato extract, sansho pepper peel or seed coat extract or hydrolysate, safflower extract, Casablanca lily extract, sweet potato extract or ferment, guava leaf extract, olive leaf extract, horse chestnut extract, ginger extract, vanilla extract, houttuynia cordata extract, pomelo extract, aloe extract, aloe vera extract, fig flower extract, apple extract, white asparagus extract, kudzu extract, codonopsis extract or hydrolysate thereof, etc.

[0041] Next, the present invention will be described in more detail with reference to manufacturing examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts refer to parts by weight, and all percentages refer to weight percent.

[0042] Preparation Example 1: Preparation of Damask rose flower extract solution The petals of the Damask rose (Rosa genus, Rosa genus, Rosaceae) were dried. 15 g of the dried petals were added to 225 g of purified water and steeped at 4°C. Then, 225 g of 1,3-butylene glycol was added. This was extracted at 4°C and then filtered to obtain 390 g of a brown, transparent Damask rose flower extract solution (solid content concentration 1.03%).

[0043] Preparation Example 2: Preparation of an extract solution from the whole plant of the Damask rose. The whole plant of the Damask rose (Rosa genus, Rosa genus, Rosaceae) was dried. 15 g of the dried plant was immersed in 225 g of purified water at 4°C, and then 225 g of 1,3-butylene glycol was added. This was extracted at 4°C and then filtered to obtain 342 g of a brown, transparent Damask rose whole plant extract solution (solid content concentration 0.62%).

[0044] Example prescription 1. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) cetyl alcohol 5.0 Methylparaben 0.1 Extract from Production Example 1 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Purified water, in an amount that makes the total volume 100 parts

[0045] Prescription example 2. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 2.0 parts of the extract from Production Example 2 were used instead of the extract from Production Example 1 contained in Formulation Example 1.

[0046] Prescription example 3. Emulsion [Ingredients] Part Squalane 5.0 Hexaran 3.0 Jojoba oil 1.0 Camellia oil 1.5 Polyoxyethylene (20) sorbitan monostearate 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Extract from Production Example 1 3.0 L-ascorbic acid-2-glucoside 2.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Sodium hyaluronate 0.01 Purified water, in an amount that makes the total volume 100 parts

[0047] Prescription example 4. Emulsion An emulsion was prepared in the same manner as in Formula Example 3, except that 3.0 parts of arbutin were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 3.

[0048] Prescription example 5. Emulsion An emulsion was obtained in the same manner as in Formula Example 3, except that 5.0 parts of nicotinamide were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 3.

[0049] Prescription example 6. Emulsion An emulsion was obtained in the same manner as in Formula Example 3, except that 2.0 parts of tranexamic acid were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 3.

[0050] Prescription example 7. Emulsion An emulsion was prepared in the same manner as in Formula Example 3, except that 2.0 parts of tranexamic acid cetyl hydrochloride were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 3.

[0051] Prescription example 8. Emulsion [Ingredients] Part Squalane 3.0 Cetyl alcohol 1.0 Polyethylene glycol monostearate 0.2 Dipropylene glycol 1.0 Cetyl isooctanoate 1.0 Dimethylpolycycloxane 1.0 Extract from Production Example 1 2.0 Lactic acid fermented rice 2.0 Coral grass extract 1.0 yeast extract 2.0 Raffinose 2.0 1,3-Butylene glycol 2.0 L-ascorbic acid-2-glucoside 2.0 Potassium hydroxide 0.5 Acetylated sodium hyaluronate 0.2 Guar gum 3.0 Ceramide 1.0 Purified water, in an amount that makes the total volume 100 parts

[0052] Prescription example 9. Emulsion An emulsion was prepared in the same manner as in Formula Example 8, except that 3.0 parts of arbutin were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 8.

[0053] Prescription example 10. Emulsion An emulsion was obtained in the same manner as in Formula Example 8, except that 5.0 parts of nicotinamide were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 8.

[0054] Prescription example 11. Emulsion An emulsion was prepared in the same manner as in Formula Example 8, except that 2.0 parts of tranexamic acid were used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the components of Formula Example 8.

[0055] Prescription example 12. Cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Behenyl alcohol 2.0 Palmitic acid 2.5 Extract from Production Example 1 2.0 Lactic acid fermented rice 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Seaweed extract 2.0 Methylparaben 0.15 Ethylparaben 0.03 Purified water, in an amount that makes the total volume 100 parts

[0056] Prescription example 13. Cream [Ingredients] Part Polyoxyethylene sorbitan monostearate 1.5 Cetyl alcohol 3.0 Glyceryl monostearate 1.0 Squalane 5.0 Olive oil 5.0 Dipropylene glycol 3.0 Extract from Production Example 2 2.0 Gentian extract 1.0 Licorice extract 2.0 Citric acid 0.2 Methylparaben 0.2 Purified water, in an amount that makes the total volume 100 parts

[0057] Example 14. Liquid Foundation [Ingredients] Part Stearic acid 2.4 Propylene glycol monostearate 2.0 Cetostearyl alcohol 0.2 Liquid lanolin 2.0 Liquid paraffin 3.0 Isopropyl myristate 8.5 Propylparaben 0.05 Extract from Production Example 1: 5.0 Sodium carboxymethylcellulose 0.2 Bentonite 0.5 Propylene glycol 4.0 Triethanolamine 1.1 Methylparaben 0.1 Titanium dioxide 8.0 Talc 4.0 Appropriate amount of coloring pigment Purified water, in an amount that makes the total volume 100 parts

[0058] Prescription Example 15: Body Shampoo [Ingredients] Part N-Lauroyl Methylalanine Sodium 25.0 Potassium coconut oil fatty acid solution (40%) 26.0 Coconut oil fatty acid diethanolamide 3.0 Methylparaben 0.1 Extract from manufacturing example 2: 5.0 1,3-Butylene glycol 2.0 Purified water, in an amount that makes the total volume 100 parts

[0059] Prescription Example 16: Hair Shampoo [Ingredients] Part N-Coconut oil fatty acid methyl taurate sodium 10.0 Sodium polyoxyethylene(3) alkyl ether sulfate 20.0 Lauryldimethylaminoacetic acid betaine 10.0 Coconut oil fatty acid diethanolamide 4.0 Methylparaben 0.1 Citric acid 0.1 Extract from Production Example 1 2.0 1,3-Butylene glycol 2.0 Purified water, in an amount that makes the total volume 100 parts

[0060] Example 17. Hair Conditioner [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Methylparaben 0.1 Extract from Production Example 2 2.0 1,3-Butylene glycol 5.0 Purified water, in an amount that makes the total volume 100 parts

[0061] Test Example 1. Evaluation of nerve growth factor (NGF) gene expression suppression. Normal human epidermal cells were grown in 6 x 10⁶ units using HuMediaKG2 [Kurabo Corporation] containing a growth additive. 4The cells / mL were prepared, and 1 mL was seeded into a 24-well plate and cultured at 37°C under 5% CO2 and saturated steam. After 1 day of culture, the extract from Production Example 1 and 100 μM epinephrine (LKT lab) were added to the culture medium to establish a test group, and epidermal cells were cultured in this culture medium. Here, the extract from Production Example 1 was added so that the final concentration as a solution was 2.0% of the total volume of the culture medium. In addition, as a comparative group, a control group was established in which only 50% 1,3-butylene glycol solution was added to the culture medium (adjusted to a final concentration of 1,3-butylene glycol of 2% of the total volume of the culture medium) instead of the extract from Production Example 1, and a control group was established in which 50% 1,3-butylene glycol solution and 100 μM epinephrine (LKT lab) were added to the culture medium. Cells from the test group were collected with 0.5 mL of Trizol reagent (Invitrogen) 3 hours after addition. 100 μL of chloroform (Wako Pure Chemical Industries, Ltd.) was added to the collected cells and mixed by stirring. The mixture was then centrifuged at 12,500 rpm at 4°C for 15 minutes using a centrifuge (TOMY MX-160), and 200 μL of the aqueous layer was collected. 500 μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the collected aqueous layer and mixed by stirring. The mixture was then centrifuged at 12,500 rpm at 4°C for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA and mixed by stirring. The precipitate was then collected by centrifugation at 12,500 rpm at 4°C for 15 minutes. The recovered total RNA was reverse transcribed using a designated kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, the expression of various genes and the expression of the internal standard G3PDH gene were detected using Thermal Cycler Dice® Real Time System Single [Takara Bio Inc.] and SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) [Takara Bio Inc.].Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is one of the housekeeping genes (genes that are commonly expressed in a constant amount in many tissues and cells, and are always expressed and essential for cell maintenance and proliferation), and because its expression level is always constant, it is used as an internal standard in PCR experiments. The test results compare the expression levels of each gene in each test group when the expression level of the G3PDH gene is kept constant. In this test system, the relative expression levels of each gene in the test group and the control group were calculated when the gene expression level in the control group was set to 100.

[0062] The results of Test Example 1 are shown in Figure 1. First, as shown in Figure 1, it was confirmed that the expression level of the NGF gene was significantly increased in the control group to which epinephrine was added as a stressor substance. On the other hand, it was confirmed that the expression of the NGF gene was significantly suppressed in the test group to which the extract according to the present invention was added compared to the control group.

Claims

1. A nerve growth factor production inhibitor containing a mixed solvent extract of water and 1,3-butylene glycol from the petals of the Damask rose (Rosa damascena) as the active ingredient (excluding steam distillate or essential oil).

2. A topical skin preparation for antipruritic or analgesic purposes, comprising the nerve growth factor production inhibitor described in claim 1.

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