Composition for improving memory or cognitive ability comprising barley extract and Oenothera biennis extract

Abstract

The present invention relates to a composition for improving memory or cognitive ability comprising green barley extract and evening primrose extract as active ingredients. More specifically, since the protective efficacy of the composition against brain nerve cells damaged by oxidative stress has been confirmed, it can be utilized in various ways, such as a composition for improving memory or cognitive ability, a health functional food, or a pharmaceutical composition.

Description

Technology Field

[0001] The present invention relates to a composition for improving memory or cognitive ability comprising green barley extract and evening primrose extract as active ingredients. Background Technology

[0002] With advancements in medical technology and improvements in living standards, average human life expectancy has nearly doubled over the past half-century, leading to a rapid increase in the proportion of the elderly population relative to the total population. Consequently, there is a growing demand for the development of functional substances and foods capable of preventing and treating cognitive impairments, including dementia.

[0003] Therefore, in order to prevent and improve degenerative brain diseases, there is a need to develop new cognitive function-enhancing substances that protect brain cells by delaying their destruction and aging, restore cognitive function, and promote brain cell regeneration. Prior art literature

[0004] 1. Patent Publication No. 10-2019-0117262 (Published Oct. 16, 2019) The problem to be solved

[0005] The objective of the present invention is to provide a composition for improving memory or cognitive ability comprising green barley extract as an active ingredient.

[0006] Another objective of the present invention is to provide a composition for improving memory or cognitive ability comprising green barley extract and evening primrose extract as active ingredients.

[0007] The objectives of the present invention are not limited to those mentioned above. The objectives of the present invention will become more apparent from the following description and will be realized by the means and combinations thereof described in the claims. means of solving the problem

[0008] To achieve the above objective, the present invention provides a composition for improving memory or cognitive ability comprising green barley extract as an active ingredient.

[0009] The above composition may additionally include evening primrose extract.

[0010] The above green barley extract and evening primrose extract may be included in a weight ratio of 1:1 to 1:4, but are not limited thereto.

[0011] The above extract may be a distilled water extract.

[0012] The above composition can protect against neuronal damage caused by oxidative stress.

[0013] In addition, the present invention provides a neuroprotective composition comprising green barley extract as an active ingredient.

[0014] The above composition may additionally include evening primrose extract.

[0015] In addition, the present invention provides a health functional food composition for improving memory or cognitive ability comprising green barley extract as an active ingredient.

[0016] In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of dementia comprising green barley extract as an active ingredient. Effects of the invention

[0017] The present invention relates to a composition for improving memory or cognitive ability comprising green barley extract and evening primrose extract as active ingredients. More specifically, since the protective efficacy of the composition against brain nerve cells damaged by oxidative stress has been confirmed, it can be utilized in various ways, such as a composition for improving memory or cognitive ability, a health functional food, or a pharmaceutical composition.

[0018] The effects of the present invention are not limited to those mentioned above. It should be understood that the effects of the present invention include all effects that can be inferred from the following description. Brief explanation of the drawing

[0019] Figure 1 shows the results of confirming the cytotoxicity of 26 types of extracts at different concentrations in brain neurons. Figure 2 confirms the results of the toxicity evaluation by H2O2 in human brain neurons. Figure 3 shows the results of confirming the cytoprotective effects of 26 types of extracts on brain neurons induced by oxidative stress. Figure 4 confirms the toxicity evaluation and cytoprotective effects of green barley extract and evening primrose extract on brain neurons induced by oxidative stress. Figure 5 shows the toxicity evaluation and cytoprotective effects of oxidative stress-induced brain neurons according to the weight ratio of green barley extract and evening primrose extract. Specific details for implementing the invention

[0020] The above objects, other objects, features, and advantages of the present invention will be easily understood through the following preferred embodiments associated with the accompanying drawings. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided to ensure that the disclosed content is thorough and complete and to ensure that the spirit of the invention is sufficiently conveyed to a person skilled in the art.

[0021] In describing each drawing, similar reference numerals have been used for similar components. In the attached drawings, the dimensions of the structures are depicted enlarged from their actual size for the clarity of the invention.

[0022] In this specification, terms such as "comprising" or "having" are intended to specify the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof. Furthermore, when a part such as a layer, film, region, or plate is described as being "on" another part, this includes not only the case where it is "immediately above" the other part, but also the case where there is another part in between. Conversely, when a part such as a layer, film, region, or plate is described as being "below" another part, this includes not only the case where it is "immediately below" the other part, but also the case where there is another part in between.

[0023] Unless otherwise specified, all numbers, values, and / or expressions used herein to represent amounts of ingredients, reaction conditions, polymer compositions, and formulations should be understood to be modified by the term “approximately” in all cases, as these numbers are essentially approximations reflecting the various uncertainties of measurement that occur in obtaining these values ​​among other things. Furthermore, where numerical ranges are disclosed herein, such ranges are continuous and, unless otherwise indicated, include all values ​​from the minimum value of such range to the maximum value including said maximum value. Moreover, where such ranges refer to integers, they include all integers from the minimum value to said maximum value including said maximum value, unless otherwise indicated.

[0025] The present invention provides a composition for improving memory or cognitive ability comprising green barley extract as an active ingredient.

[0026] Green barley Hordeum vulgareBarley (L.) is one of the world's four major crops belonging to the Poaceae / Gramineae family. Unlike ordinary barley, which has a yellowish tint, it refers to barley that is still unripe and has a greenish tint. The term "unripe" above may refer to the state where the barley fruit is immature, and in the case of Korea, it may refer to barley during the period from March to May before harvest. In particular, barley leaves are known to be rich in various physiologically active substances, such as the antioxidant superoxide dismutase (SOD), polyphenol compounds, vitamin C, vitamin E, catechin, and β-carotene.

[0027] The above composition may additionally include evening primrose extract.

[0028] The above green barley extract and evening primrose extract may be included in a weight ratio of 1:1 to 1:4, preferably 1:1. When the weight ratio falls within the above numerical range, the protective effect against neuronal damage caused by oxidative stress is excellent.

[0029] The above nerve cell may be a brain nerve cell.

[0030] The above composition can protect against neuronal damage caused by oxidative stress.

[0031] The above oxidative stress may be induced by H2O2.

[0032] In addition, the present invention provides a neuroprotective composition comprising green barley extract as an active ingredient.

[0033] The above composition may additionally include evening primrose extract.

[0034] In addition, the present invention provides a health functional food composition for improving memory or cognitive ability comprising green barley extract as an active ingredient.

[0035] The above health functional food may contain various nutritional supplements, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc.

[0036] In addition, it may contain fruit pulp for the production of natural fruit juice, synthetic fruit juice, and vegetable drinks. These ingredients may be used independently or in combination. Furthermore, the health functional food composition may be in the form of any one of meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, chewing gum, ice cream, soup, beverage, tea, functional water, drinkables, alcohol, and vitamin complexes.

[0037] In addition, the above-mentioned health functional food may further include food additives, and unless otherwise stipulated, suitability as a "food additive" shall be determined in accordance with the specifications and standards for the relevant item, based on the general provisions and general test methods of the Food Additives Codex approved by the Ministry of Food and Drug Safety.

[0038] Examples of items listed in the above "Food Additives Codex" include chemically synthesized products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamon acid; natural additives such as persimmon dye, licorice extract, crystalline cellulose, kohlrabi dye, and guar gum; L-sodium glutamate preparations; alkaline agents added to noodles; preservative preparations; and mixed preparations such as tar dye preparations.

[0039] At this time, the content of the active ingredient added to the food during the process of manufacturing the health functional food can be appropriately increased or decreased as needed, and preferably, it can be added in an amount of 1 to 90 parts by weight per 100 parts by weight of the food.

[0040] In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of dementia comprising green barley extract as an active ingredient.

[0041] The dosage of the active ingredient according to the present invention may vary depending on the subject's condition and body weight, the type and severity of the disease, the form of the drug, the route of administration, and the duration, and may be appropriately selected by a person skilled in the art, and the daily dosage may be 0.01 mg / kg to 200 mg / kg, preferably 0.1 mg / kg to 200 mg / kg, more preferably 0.1 mg / kg to 100 mg / kg. The administration may be performed once a day or divided into several doses, and the scope of the present invention is not limited by this.

[0043] The present invention will be explained in detail below through examples and comparative examples. However, the following examples and comparative examples are merely illustrative of the present invention, and the scope of the present invention is not limited to the following examples.

[0045] <Example 1> Extraction, concentration, and freeze-drying of green barley and 25 other candidate substances

[0046] For the preparation of freeze-dried products, the raw material was extracted with 10 to 20 times the amount of distilled water at 80°C for 2 hours, and then filtered using filter paper. The filtrate was concentrated under reduced pressure and freeze-dried for 48 hours, and the final extract was used as the sample for the experimental example. The types of extracts obtained with purified water are indicated in Table 1 below.

[0047] No. Sample name No. Sample name 1 persimmon tree 14 Island aster 2 Artemisia annua 15 Thistle 3 master 16 Acanthopanax 4 Evening primrose 17 Ore tree 5 quince tree 18 bay tree 6 water parsley 19 clove tree 7 basil 20 Jipshinnamul 8 mint 21 green barley 9 Korean ash (mountain ash) 22 kudzu 10 Korean black raspberry 23 Chamomile 11 Bisuri 24 time 12 Artemisia annua 25 Hyunggae 13 Western peppermint 26 fennel

[0049] <Example 2> Evaluation of Cytotoxicity of 26 Extracts at Different Concentrations on SH-SY5Y Cells

[0050] To confirm the protective efficacy in brain neurons induced by oxidative stress, a toxicity evaluation was conducted using SH-SY5Y cells.

[0051] SH-SY5Y cells (neuroblastoma) were purchased from the Korean Cell Line Bank, and the SH-SY5Y cells were added to Dulbecco's Modified Eagle Medium: Nutrient Mixture F12 (DMEM F12) medium with 10% heat-inactivated FBS and 1% antibiotics, and then cultured at 37°C in a 5% CO2 incubator.

[0052] The cytotoxicity of the extract in human brain neurons was evaluated using the MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2Htetrazolium bromide) reagent. Samples were treated in 96-well plates at concentrations of 250 μg / mL and 500 μg / mL for 24 hours. After incubating in an incubator at 37°C for 24 hours, the supernatant was removed, MTT solution (0.5 mg / ml) was added, and after incubation for 1 hour, 100 μL of 100% DMSO was added to dissolve the formed formazan, and the absorbance was measured at 595 nm using an ELISA microplate reader.

[0053] As a result, according to Figure 1, it was confirmed that most extracts were toxic at 500 μg / mL, and for subsequent neuroprotective effect experiments, 250 μg / mL was selected as the final extract concentration.

[0055] <Example 3> Measurement of protective effect in brain neurons induced by oxidative stress

[0056] 1. Experimental Method

[0057] H2O2 induction in human brain neurons (SH-SY5Y) to confirm the protective effect on brain neurons induced by oxidative stress was carried out as follows.

[0058] First, H2O2 was treated at concentrations ranging from 10 to 1000 μM to find the concentration that damages 40-60% of brain neurons, and as a result, according to Figure 2, oxidative stress was induced by treating at the optimal concentration of 50 μM.

[0059] The H2O2-induced cytoprotective effects of the 26 types of extracts were confirmed by performing an MTT.

[0060] Samples of the above-prepared extracts were treated at a concentration of 250 μg / mL and cultured for 24 hours. Afterward, 250 μM of H2O was irradiated and cultured for 2 hours. As with the MTT assay described above, after adding an MTT solution (1 mg / ml) and cultured for 2 hours, the formazan crystals were dissolved in DMSO, and the absorbance was measured at 595 nm using an ELISA microplate reader.

[0062] 2. Experimental Results

[0063] As a result, according to Figure 3, human brain neurons treated with H2O2 showed a cell survival rate of 59.1%. When treated with each extract, excellent cell protective effects of 72.0%, 94.1%, and 70.1% were confirmed for evening primrose extract, persimmon extract, and water parsley extract, respectively.

[0066] <Example 4> Evaluation of Toxicity of Extract Complex on Oxidative Stress-Induced Neuronal Cells and Measurement of Cytoprotective Effect

[0067] 1. Experimental Method

[0068] Experiments were conducted on extract complexes to confirm the protective effect on brain neurons induced by oxidative stress. In the above Example 3, a complex was prepared using three excellent materials (evening primrose, persimmon tree, and water parsley) and green barley, and the experiment was conducted. The cytotoxicity and protective effects were measured using the same method as in the above Examples 2 and 3.

[0070] 2. Experimental Results

[0071] As a result, according to Figure 4, the three complexes (green barley persimmon complex, green barley water parsley complex, and green barley evening primrose complex) did not show cytotoxicity at any of the analyzed concentrations (125, 250, 500 ug / ml).

[0072] Furthermore, regarding the results of cytoprotective effects, human brain neurons treated with H2O2 showed a cell viability of 53% compared to the control group; however, the green barley persimmon complex demonstrated protective effects of 69.3%, 51.8%, and 48.3% at different concentrations, the green barley water parsley complex showed 48.1%, 52.9%, and 60.9%, and the green barley evening primrose complex showed 71.7%, 55.5%, and 40.7%. Among the extract complexes, the green barley evening primrose complex was identified as the most superior material in terms of neuronal protection against oxidative stress.

[0074] <Example 5> Evaluation of Toxicity and Measurement of Cytoprotective Effects on Oxidative Stress-Induced Neuronal Cells by Ratio of Blue Barley Evening Primrose Complex

[0075] 1. Experimental Method

[0076] Experiments were conducted on extract complexes to confirm the protective effect on brain neurons induced by oxidative stress. The excellent barley evening primrose complex from Example 4 was prepared according to different ratios and the experiment was conducted. The cytotoxicity and protective effects were measured using the same method as in Examples 2 and 3.

[0078] 2. Experimental Results

[0079] As a result, according to Figure 5, the ratios of the evening primrose complex at 1:1, 1:2, and 1:4 (weight ratio) did not show cytotoxicity at any of the analyzed concentrations (125, 250, 500 ug / ml).

[0080] In addition, regarding the results of the cell protective effects, when treated with H2O2 in human brain neurons compared to the control group, the green barley extract showed a cell protective effect of 98.5%, and the evening primrose extract showed 121.8%. In the green barley and evening primrose complex, at a weight ratio of 1:1, protective effects of 156.5%, 111.2%, and 109.0% were observed compared to H2O2 treatment at different concentrations; at 1:2, protective effects of 94.0%, 79.2%, and 80.5% were observed; and at 1:4, protective effects of 134.5%, 115.5%, and 91.4% were observed. Among the protective effects of the extract complex against oxidative stress, the green barley and evening primrose complex at a weight ratio of 1:1 was confirmed to be the most superior material, rather than the single extracts of green barley and evening primrose.

[0082] The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art will understand that other specific forms can be easily modified without altering the technical spirit or essential features of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive.

[0083] The scope of the present invention is defined by the claims set forth below, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts thereof should be interpreted as being included within the scope of the present invention.

Claims

Claim 1 A composition for improving memory or cognitive ability containing green barley extract as an active ingredient. Claim 2 A composition for improving memory or cognitive ability according to claim 1, characterized in that the composition further comprises evening primrose extract. Claim 3 A composition for improving memory or cognitive ability according to claim 2, characterized in that the green barley extract and evening primrose extract are included in a weight ratio of 1:1 to 1:

4. Claim 4 A composition for improving memory or cognitive ability according to claim 1, wherein the composition protects against neuronal damage caused by oxidative stress. Claim 5 A neuroprotective composition containing green barley extract as an active ingredient. Claim 6 A neuroprotective composition according to claim 5, characterized in that the composition further comprises evening primrose extract. Claim 7 A health functional food composition for improving memory or cognitive ability containing green barley extract as an active ingredient. Claim 8 A pharmaceutical composition for the prevention or treatment of dementia comprising green barley extract as an active ingredient.

Images