Transgenic soybean event GM_CSM63717 and composition and method for detection and use thereof

Abstract

Transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, offspring plants, and agricultural and commercial products containing event Gm_CSM63717 are also provided. A recombinant DNA molecule unique to event Gm_CSM63717, and methods for the use and detection of Gm_CSM63717 are also provided. Soybean plants containing event Gm_CSM63717 exhibit resistance to PPO inhibitors.

Description

Technology Field

[0001] Cross-reference regarding related applications

[0002] This application claims priority to U.S. provisional application No. 63 / 609,784 filed on December 13, 2023, filed on December 20, 2022, the full disclosure of which is incorporated herein by reference.

[0003] Inclusion in sequence list

[0004] The sequence list contained in filename "MONS572WO_ST26.xml" is 293 kilobytes (measured on MS-Windows), was created on October 1, 2024, and is submitted herein by electronic submission, the whole of which is incorporated herein by reference.

[0005] The present disclosure relates to compositions and methods for providing herbicide resistance in transgenic soybean plants. Recombinant DNA molecules present in and / or isolated therefrom soybean event GM_CSM63717 are provided. Additionally, transgenic soybean plants, plant parts, seeds, cells, and agricultural products containing soybean event Gm_CSM63717 are provided, as well as methods for the production and use of transgenic soybean plants, plant parts, seeds, cells, and agricultural products containing soybean event Gm_CSM63717, methods for detecting soybean event Gm_CSM63717, and methods for weed control are provided. Transgenic soybean plants, plant parts, seeds, and cells containing soybean event Gm_CSM63717 exhibit resistance to various PPO herbicides. Background Technology

[0006] Increasing sustainable crop production is crucial for meeting the food demands of a growing global population while utilizing limited natural resources, addressing the rising demand for animal-based diets in developing countries, and expanding the use of crop production for biofuels, fibers, and other agri-based commodities. In agricultural systems, the effective management of weed species on farmland is essential for maintaining favorable crop growing conditions and yields. Weeds compete with crops for space, nutrients, water, and light, and can contaminate harvests, presenting one of the major challenges for sustainable crop production. Over an average of seven years from 2007 to 2013, weed interference in soybeans in the U.S. and Canada resulted in an average yield loss of 52.1%, which corresponds to an annual loss of approximately $17.1 billion if weed management tactics were not employed (Soltani et al., 2017). Furthermore, yield losses caused by weeds are influenced by weather. Under drought conditions, many weed species exhibit increased competitiveness (Patterson, 1995). Selective herbicides have contributed significantly to weed management prior to the deployment of herbicide-resistant crops. Herbicide application provides an important tool for reducing weed pressure on global crop production, improving productivity, and increasing security.

[0007] Soybeans Glycine max ) is an important crop in many parts of the world. The introduction of genetically modified crops containing herbicide resistance traits has successfully provided farmers with an additional tool available to better control weeds. Transgenic herbicide resistance allows for the control of crops without or with minimal crop damage ( for exampleTransgenic soybean traits (with less than 10% damage) enable the use of herbicides in crop growing environments. Transgenic soybean traits have been used to confer resistance to glyphosate, glufosinate, and 2,4-D and are widely used in commercial soybean production for weed control. However, weeds have evolved resistance to herbicides, and weed resistance continues to present challenges in soybean production today. Therefore, additional herbicide resistance trait options are needed to effectively manage weeds and sustain crop productivity. One solution is to use herbicide(s) with novel or different modes of action, and / or to use multiple herbicides with different modes of action.

[0008] Protoporpyrinogen IX oxidase (PPO) catalyzes the oxidation of protoporpyrinogen IX to produce protoporphyrin IX. This enzymatic step is conserved across prokaryotes and eukaryotes for the synthesis of tetrapyrroles such as heme. In plants, chlorophyll production depends on this PPO-catalyzed reaction because protoporphyrin IX is an important precursor for chlorophyll synthesis. Due to the critical role of PPO in plant chlorophyll synthesis, various protoporpyrinogen oxidase (PPO) inhibitor herbicides have been developed and used for weed control in agriculture since the 1960s. The application of PPO inhibitor herbicides to sensitive plants results in the blockade of heme and chlorophyll biosynthetic pathways in plastids, leading to the accumulation of pathway intermediates that leak from plastids and undergo non-specific oxidation to protoporphyrin IX in the cytoplasm. In the presence of oxygen and light, protoporphyrin IX rapidly generates singlet oxygen, leading to uncontrolled membrane lipid peroxidation and plant death. The development of PPO-inhibiting herbicide resistance traits through biotechnology will provide farmers with additional tools and additional herbicide modes of action to diversify weed control systems to control weeds and reduce / prevent the development of herbicide resistance (Larue et al, 2019). These PPO herbicide resistance traits can be deployed alone or stacked with other herbicide resistance traits.

[0009] Combinations of herbicide resistance traits are desirable for providing weed control options that increase grower flexibility and enable the use of multiple herbicide modes of action to control difficult weeds. Combining multiple desired traits within the genome can be achieved by the following approaches: 1) crossing two parents each possessing the desired trait at randomly inserted sites and identifying offspring plants with a combination of the desired traits; 2) retransforming transgenic plants containing one or more desired trait(s) into one or more genes for additional desired traits through random integration or through the targeted integration of one or more genes for additional desired traits; 3) inserting multiple genes as a single DNA molecule into a single location or locus within the genome, which provides a useful tool for effective weed control that is much simpler and less expensive to maintain during subsequent breeding into various grasses of elite germplasm; and 4) targeting one or more desired traits in a new transformation event to a specific genomic location (site-specific integration) that possesses one or more desired traits, and then crossing the new event with another event that possesses one or more desired traits at the specific genomic location to produce offspring plants having a combination of desired traits at one location and isolating them together.

[0010] The expression of a transgene and its resulting effects in transgenic plants, plant parts, seeds, cells, or offspring can be influenced by many factors, such as the regulatory elements used in the transgene expression cassette, the combination and / or interaction of these regulatory elements, the chromosomal location of the transgene insertion site, the chromatin structure of the genome at or near the transgene insertion site, and the presence or proximity of any endogenous cis and / or trans regulatory elements or genes close to the transgene insertion site. Furthermore, the performance of traits in transgenic plants becomes additionally complicated when the transgene insertion comprises multiple expression cassettes containing different transgenes, each conferring distinct traits. These differences or factors can lead to variations in the transgene expression levels or the spatial or temporal patterns of transgene expression between different transgene insertion events within the same expression cassette. Moreover, different transgene events may also differ in terms of the molecular quality of the event. For example, a transformation event may contain two or more copies of a transformation gene insert at one or more chromosomal locations, the transformation insert may be truncated relative to the intended insert or contain a vector backbone sequence, or the transformation gene may be inserted into an endogenous gene or a repetitive region. In the case of site-specific integration of a desired trait, machinery for site-specific integration, such as gRNA or nucleases that must be excised from the commercial event, may not be completely removed. These characteristics may lead to undesirable consequences, such as gene silencing, altered patterns and / or expression of the transformation gene, and / or altered patterns and / or expression of the endogenous gene. Additionally, there may be undesirable phenotypic or agronomic differences between different events.

[0011] Even in the case of targeted sequence insertions, variability in the expression levels of transgenic genes between independent but genetically identical targeted sequence insertion (TSI) events has been observed in a subset of transgenic events (Verkest et al., 2019). This expression variability and silencing may be attributed to DNA methylation that occurred independently of the transgenic gene sequence and is further linked to different DNA methylation mechanisms. Integration of transgenic genes into targeted loci via Cre-lox-mediated recombination has also been reported to generate a large percentage of targeted integration events, exhibiting partial spatial patterns of transgenic gene expression due to differential silencing (Day et al., 2000). The fact that significant variability in transgenic gene expression has been observed demonstrates that even when integration events are targeted, selection remains necessary—similar to the practice for random integration events—to identify targeted insertion events that are stable over generations and correspond to desirable gene expression of interest.

[0012] Commercially useful transformation events require that the transformation gene(s) within the transformation insert be expressed in a manner necessary for the success of the corresponding trait, and that this involves rigorous testing, evaluation, and selection. Such testing involves different regulatory elements (e.g., promoters, introns, leaders, and 3' UTRs) and combinations of different regulatory elements regarding the desired spatial and temporal expression of the transformation gene(s). In a test tube and / or inside plantsIt includes not only testing but also investigating whether the optimal expression cassette(s) are selected by targeting the products of the transformed gene(s) (protein(s)) to subcellular compartments such as chloroplasts. For site-specific integration of the transformed gene, once a targeted insertion strategy / method is selected, target sites are identified, screened, and selected. Then, the selected expression cassette(s) combination, target site, and gRNA are used for transformation to produce transformed plants.

[0013] For this reason, the performance of different transformation events from the same transforming compost can vary widely, and identifying the transformation event that confers the most beneficial trait or characteristic without other potential defects or concerns is necessary to select the superior event for commercial use. Therefore, a large number of individual transformation events must be produced and analyzed to select an event with superior commercial characteristics, which can be an important task involving analysis and selection among many different transformation events.

[0014] To establish a transgenic event for commercial use, rigorous molecular characterization, greenhouse testing, and field trials are required over several years across various regions and under diverse conditions, allowing for the acquisition of extensive agronomic, phenotypic, and molecular data. The generated data is then analyzed to select an event suitable for commercial purposes. Once the commercial event is identified as possessing the desired transgenic gene expression, molecular characteristics, efficacy, and field performance, it can be introgressed into other soybean genetic backgrounds using plant breeding methods. The resulting soybean varieties contain novel traits combined with other desired characteristics, such as native traits, disease resistance traits, pest control traits, high-yield germplasm or traits, and / or one or more other transgenic herbicide resistance traits.

[0015] A recombinant DNA molecule is provided herein. Examples of such recombinant DNA molecules include a nucleotide sequence selected from the group consisting of SEQ ID NO. 10, SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, and SEQ ID NO. 9; a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the entire length of SEQ ID NO. 10 or the entire length of SEQ ID NO. 9; and a recombinant DNA molecule comprising a complete complement of any of the above. In some embodiments, the recombinant DNA molecule is derived from a soybean plant, seed, plant part, plant cell, offspring plant, or commercial product containing soybean event Gm_CSM63717, which is a representative sample of seed containing the event deposited under ATCC accession number PTA-127604. In some embodiments, the recombinant DNA molecule is contained in a soybean plant, seed, plant part, plant cell, or offspring plant, or a commercial product produced therefrom, containing soybean event Gm_CSM63717, which is a representative sample of seed containing the event deposited under ATCC accession number PTA-127604. The recombinant DNA molecule may be formed by inserting a heterologous nucleic acid molecule into the genomic DNA of a soybean plant or soybean cell. The recombinant DNA molecule may include an amplicon diagnosis for the presence of soybean event Gm_CSM63717.

[0016] A DNA molecule that functions as a DNA probe is provided. An example of such a DNA molecule is a DNA molecule containing a polynucleotide segment of sufficient length to function as a DNA probe that specifically hybridizes with the soybean event Gm_CSM63717 DNA in a sample under strict hybridization conditions. Detecting the hybridization of the DNA molecule under strict hybridization conditions is a diagnosis of the presence of the soybean event Gm_CSM63717 in the sample.

[0017] Additionally, a DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe specific for detecting at least one of the following in a sample is provided: a 5' junction sequence between the lateral soybean genomic DNA and the transform insert of the soybean event Gm_CSM63717; a 3' junction sequence between the transform insert of the soybean event Gm_CSM63717 and the lateral soybean genomic DNA; SEQ ID NO. 9; and a fragment of SEQ ID NO. 9 comprising adjacent nucleotides of SEQ ID NO. 9 of sufficient length to identify the sequence as a fragment of the transform insert of Gm_CSM63717.

[0018] The DNA probe may include SEQ ID NO. 178. Alternatively or additionally, the DNA probe may include a nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, and a complement of any of the above. The sample may be derived from a soybean plant, seed, plant part, plant cell, offspring plant, or commercial product.

[0019] A pair of DNA molecules is provided. The pair of DNA molecules comprises a first DNA molecule and a second DNA molecule. The first and second DNA molecules are distinct from each other and each comprises a fragment of SEQ ID NO. 10 or its complement and functions as a DNA primer when used together in an amplification reaction with DNA containing the soybean event Gm_CSM63717 to produce an amplicon diagnosis for the soybean event Gm_CSM63717 in a sample. For example, the first and second DNA molecules may comprise SEQ ID NO. 176 and SEQ ID NO. 177. The amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO. 1; SEQ ID NO. 2; SEQ ID NO. 3; SEQ ID NO. 4; SEQ ID NO. 5; SEQ ID NO. 6; SEQ ID NO. 7; SEQ ID NO. 8; SEQ ID NO. 9; and SEQ ID NO. 10; It may include a fragment of any of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, and SEQ ID NO. 8, wherein the fragment is at least 10 nucleotide long and includes nucleotides 1,000-1,001 or 4,201-4,202 of SEQ ID NO. 10.

[0020] A method for detecting the presence of soybean event Gm_CSM63717 in a sample derived from soybean seeds, plants, plant parts, plant cells, offspring plants, or commercial products is provided. In a first example of such a method, the method comprises: a) contacting a sample with any of the DNA molecules functioning as DNA probes specific to soybean event Gm_CSM63717 as described herein; b) applying the sample and the DNA molecule functioning as a probe to strict hybridization conditions; and c) detecting hybridization of the DNA molecule functioning as a probe to the DNA molecule in the sample. Hybridization of the DNA molecule functioning as a probe to the DNA molecule in the sample is a diagnosis of the presence of soybean event Gm_CSM63717 in the sample.

[0021] Another method is provided for detecting the presence of soybean event Gm_CSM63717 in a sample derived from soybean seeds, plants, plant parts or plant cells, offspring plants or commercial products. The method comprises: a) contacting the sample with any pair of DNA molecules that can be used to produce an amplicon diagnosis for soybean event Gm_CSM63717 as described herein; b) performing an amplification reaction sufficient to produce a DNA amplicon; and c) detecting the presence of a DNA amplicon. The DNA amplicon comprises at least one of the following: a 5' junction sequence between the lateral soybean genomic DNA and a transform insert of soybean event Gm_CSM63717; a 3' junction sequence between the lateral soybean genomic DNA and soybean event Gm_CSM63717, SEQ ID NO. 9, and a fragment of SEQ ID NO. 9 comprising adjacent nucleotides of SEQ ID NO. 9 of a length sufficient to identify the sequence as a fragment of the transform insert of Gm_CSM63717. The presence of a DNA amplicon indicates the presence of the soybean event Gm_CSM63717 in the sample. The DNA amplicon may be at least 10 nucleotide lengths, at least 11 nucleotide lengths, at least 12 nucleotide lengths, at least 13 nucleotide lengths, at least 14 nucleotide lengths, at least 15 nucleotide lengths, at least 16 nucleotide lengths, at least 17 nucleotide lengths, at least 18 nucleotide lengths, at least 19 nucleotide lengths, at least 20 nucleotide lengths, at least 25 nucleotide lengths, at least 30 nucleotide lengths, at least 35 nucleotide lengths, at least 40 nucleotide lengths, at least 45 nucleotide lengths, at least 50 nucleotide lengths, at least 60 nucleotide lengths, at least 70 nucleotide lengths, at least 80 nucleotide lengths, at least 90 nucleotide lengths, or at least 100 nucleotide lengths.The DNA amplicon may include a nucleotide sequence selected from the group consisting of SEQ ID NO 10; SEQ ID NO 9; SEQ ID NO 8; SEQ ID NO 7; SEQ ID NO 6; SEQ ID NO 5; SEQ ID NO 4; SEQ ID NO 3; SEQ ID NO 2; and SEQ ID NO 1; and a fragment of any of SEQ ID NO 10, SEQ ID NO 8, SEQ ID NO 7, SEQ ID NO 6, SEQ ID NO 5, SEQ ID NO 4, SEQ ID NO 3, SEQ ID NO 2, and SEQ ID NO 1, which is at least 10 nucleotide long and contains 1,000-1,001 or 4,201-4,202 nucleotides of SEQ ID NO 10.

[0022] An additional method is provided for detecting the presence of soybean event Gm_CSM63717 in a sample of DNA derived from soybean seeds, plants, plant parts, plant cells, offspring plants, or commercial products. The method comprises: a) contacting the sample with any of the DNA molecules that function as probes specific to the soybean event Gm_CSM63717 described herein; and b) performing a sequencing reaction to produce a target sequence. The target sequence comprises a nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, the complete complement of any of these, and a fragment of any of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, and SEQ ID NO. 10 that is at least 10 nucleotide long and contains 1,000-1,001 or 4,552-4,553 nucleotides of SEQ ID NO. 10.

[0023] Another method is provided for detecting the presence of soybean event Gm_CSM63717 in a sample derived from soybean seeds, plants, plant parts, cells, offspring plants, or commercial products. The method comprises the steps of: (a) contacting the sample with an antibody specific to the PPO (protoporpyrinogen oxidase) protein encoded by soybean event Gm_CSM63717; and (b) detecting the binding of the antibody to the protein in the sample. The binding of the antibody indicates the presence of soybean event Gm_CSM63717 in the sample.

[0024] A DNA detection kit for detecting the presence of soybean event Gm_CSM63717 in a sample is provided. One example of such a DNA detection kit is a kit comprising any of the pairs of DNA primers that can be used as primers to produce an amplicon diagnosis for soybean event Gm_CSM63717 described herein. Another example of a DNA detection kit is the soybean event Gm_CSM63717 described herein

[0025] Additionally, a protein detection kit is provided to detect the presence of soybean event Gm_CSM63717 in a sample. One example of such a kit is one containing an antibody specific to the PPO protein encoded by soybean event Gm_CSM63717. Detecting the binding of the antibody to the protein encoded by soybean event Gm_CSM63717 in a sample is a diagnosis of the presence of soybean event Gm_CSM63717 in the sample.

[0026] A method for determining the zygosity of a soybean plant, plant part, plant seed, or plant cell containing soybean event Gm_CSM63717 is provided. One example of such a method comprises: a) contacting a sample containing DNA derived from a soybean plant, plant part, plant seed, or plant cell with a first set of primers capable of producing a first amplicon diagnosis for the presence of soybean event Gm_CSM63717 and a second set of primers capable of producing a second amplicon diagnosis for wild-type soybean genomic DNA not containing soybean event Gm_CSM63717; b) performing a nucleic acid amplification reaction; and c) detecting the first amplicon and the second amplicon. The presence of both amplicons indicates that the plant, plant part, seed, or cell is heterozygous for soybean event Gm_CSM63717. The presence of only the first amplicon indicates that the plant, plant part, seed, or cell is homozygous for the soybean event Gm_CSM63717. For example, the first primer set may include SEQ ID NO. 176 and SEQ ID NO. 177, and the second primer set may include SEQ ID NO. 208 and SEQ ID NO. 209.

[0027] Another method is provided for determining the zygosity of a soybean plant, plant part, plant seed, or plant cell containing soybean event Gm_CSM63717. The method comprises: a) contacting a sample containing DNA derived from a soybean plant, plant part, plant seed, or plant cell with a probe set comprising at least a first probe that specifically hybridizes to soybean event Gm_CSM63717, and at least a second probe that specifically hybridizes to soybean genomic DNA that is disrupted by heterologous DNA insertion of soybean event Gm_CSM63717 but does not hybridize to soybean event Gm_CSM63717; and b) hybridizing the probe set with the sample under strict hybridization conditions. Detecting hybridization of only the first probe under hybridization conditions is a diagnosis of a soybean plant, plant part, seed, or plant cell that is homozygous to soybean event Gm_CSM63717. Detecting the hybridization of both the first probe and the second probe under hybridization conditions is a diagnosis of a soybean plant, plant part, seed, or plant cell that is heterozygous for the soybean event Gm_CSM63717. For example, the probe set may include SEQ ID NO. 178 and SEQ ID NO. 210.

[0028] A DNA construct is provided. One example of a DNA construct provided herein is a DNA construct comprising an expression cassette, wherein the expression cassette comprises the following via operable connections: i) Medicago truncatula ii) ubiquitin 2 (UBQ) promoter, leader sequence, and intron sequence from Adansonia digitata iii) Codon-optimized chloroplast shift peptide coding sequence of APG6 (albino and pale green 6) from Enterobacter cloacaeiv) a codon-optimized protoporpyrinogen oxidase coding sequence from, and iv) a 3' UTR sequence of the synthetic gene. For example, a DNA construct includes SEQ ID NO. 9. The DNA construct comprises (a) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 11 or SEQ ID NO. 14; and / or (b) may additionally include at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of sequence number 12 or sequence number 15.

[0029] Another DNA construct is provided. The DNA construct comprises a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the total length of SEQ ID NO. 9. The DNA construct comprises SEQ ID NO. 9.The DNA construct comprises (i) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 11 or SEQ ID NO. 14; and / or (ii) comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 12 or SEQ ID NO. 15.

[0030] Any of the DNA constructs may include one or more nucleotide sequences selected from SEQ ID NOs 36-105 at the 5' end of the said construct. Any of the DNA constructs may include one or more nucleotide sequences selected from SEQ ID NOs 106-175 at the 3' end of the said construct.

[0031] A method for controlling or preventing weed growth in a region is provided. One example of such a method includes the step of sowing soybeans containing event Gm_CSM63717 in the region and the step of controlling weeds in the region by applying an effective amount of PPO herbicide without damage to the soybeans or with less than about 10% damage to the soybeans.

[0032] A method for controlling volunteer soybeans containing soybean event Gm_CSM63717 in a region is provided. One example of such a method comprises the step of applying a herbicidally effective amount of at least one herbicide other than a PPO herbicide, wherein the application of the herbicide prevents the growth of soybeans containing soybean event Gm_CSM63717. The herbicide other than a PPO herbicide may be selected from the group consisting of atrazine, topramezone, clopyralide, pyritiobak, fluoromethurone, (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU), triploxisulfurone, paraquat, diquat, glyphosate, glufosinate, and any combination thereof.

[0033] A method for obtaining seeds or soybean plants resistant to PPO herbicides is provided. One example of such a method comprises a) obtaining a population of offspring seeds or plants grown therefrom, at least one of which contains soybean event Gm_CSM63717; and b) identifying at least a first offspring seed or plant grown therefrom which contains soybean event Gm_CSM63717. Identifying offspring seeds or plants grown therefrom which contain soybean event Gm_CSM63717 may include (a) growing offspring seeds or plants to produce offspring plants; (b) treating offspring plants with an effective amount of PPO herbicide; and (c) selecting offspring plants resistant to PPO herbicides. Alternatively or additionally, identifying offspring seeds containing soybean event Gm_CSM63717 or plants grown therefrom may include detecting the presence of soybean event Gm_CSM63717 in samples derived from offspring seeds or plants grown therefrom. Alternatively or additionally, identifying offspring seeds containing soybean event Gm_CSM63717 or plants grown therefrom includes detecting the presence of a PPO protein encoded by soybean event Gm_CSM63717 in samples derived from offspring seeds or plants grown therefrom.

[0034] A method for improving resistance to PPO herbicides in soybean plants is provided. One example of such a method comprises: a) inserting any of the DNA constructs described herein into the genome of a soybean cell; b) generating a soybean plant from the soybean cell; and c) selecting a soybean plant containing the DNA construct. The selecting step may include treating the soybean cell or plant with an effective amount of PPO herbicide.

[0035] A soybean plant, a plant seed, a plant part, and a plant cell comprising a recombinant DNA molecule are provided. The recombinant DNA molecule is a polynucleotide having a sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9; and the whole length of SEQ ID NO. 10, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the whole length of SEQ ID NO. 10 or the whole length of SEQ ID NO. 9; and includes the complete complement of any of the foregoing. A soybean plant, plant seed, plant part, or plant cell expresses a PPO herbicide resistance gene. A soybean plant, plant seed, plant part, or plant cell is resistant to one or more PPO herbicides.

[0036] Additional soybean plants, plant seeds, plant parts, and plant cells are provided. The soybean plants, plant seeds, plant parts, or plant cells are resistant to one or more PPO herbicides and comprise any of the DNA constructs described herein.

[0037] A soybean plant, plant seed, plant part, or plant cell may additionally contain at least one additional transgenic gene for resistance to at least one additional herbicide.

[0038] Any of the soybean plant, plant seed, plant part, or plant cell may contain soybean event Gm_CSM63717, which is a representative sample of a seed containing the event deposited under ATCC accession number PTA-127604.

[0039] Any of the soybean plant, plant seed, plant part, or plant cell may be further defined as a progeny plant of any generation of the soybean plant containing the soybean event Gm_CSM63717, or a soybean plant part, plant seed, or plant cell derived therefrom.

[0040] Additional soybean plants, plant parts, plant seeds, or plant cells are provided. The soybean plants, plant parts, plant seeds, or plant cells include soybean event Gm_CSM63717, which is a representative sample of seeds containing soybean event Gm_CSM63717 deposited under ATCC accession number PTA-127604.

[0041] The parts of the soybean plant may include microspores, pollen, anthers, ovules, ovaries, pods, flowers, embryos, stems, buds, nodes, leaves, roots, or calluses.

[0042] Any of a soybean plant, plant seeds, plant parts, or plant cells may be obtained by any of the methods for obtaining seeds or soybean plants resistant to PPO herbicides, or any of the methods for improving resistance to PPO herbicides in soybean plants described herein.

[0043] Additional soybean plants, plant cells, plant parts, or plant seeds are provided. The soybean plants, plant cells, plant parts, or plant seeds comprise a recombinant DNA construct incorporated into chromosome 13. The recombinant DNA construct confers resistance to at least one PPO herbicide. A recombinant DNA construct is incorporated at the location of the chromosome, and on its side at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 11 or SEQ ID NO. 14; and / or (ii) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 12 or SEQ ID NO. 15. At least 50 adjacent nucleotides of sequence number 11 or sequence number 14 may include one or more nucleotide sequences selected from sequence numbers 36-105.At least 50 adjacent nucleotides of sequence number 12 or sequence number 15 comprise one or more nucleotide sequences selected from sequence numbers 106-175.

[0044] With respect to any of soybean plants, plant cells, plant parts, or plant seeds resistant to one or more PPO herbicides, any of methods for controlling or preventing weed growth in an area including applying an effective amount of PPO herbicide, any of methods for obtaining seeds or soybean plants resistant to PPO herbicides, and any of methods for improving resistance to PPO herbicides in soybean plants, the PPO herbicide may be selected from the group consisting of diphenyl ether, N-phenylphthalimide, oxadiazole, oxazolidindione, phenylpyrazole, pyrimidindinone, thiadiazole, triazolinone, benzoxazinone derivatives, other PPO herbicides, and any combination thereof. Diphenyl ether may be selected from the group consisting of acifluorophene, bifennox, ethoxyphene, fluorodiphene, fluoronitrophene, furyloxyphene, halosafene, clomethoxyphene, chlornitrophene, ethoxyphene-ethyl, fluoroglycopene, lactophene, nitrophene, oxyfluorophene, fomesafene, any salt thereof, and any ester thereof. N-phenylphthalimide may be selected from the group consisting of cinidone-ethyl, flumiclorac, flumiclorac-pentyl, and flumioxazine. Oxadiazole may be selected from the group consisting of oxadiargyl and oxadiazone. Oxazolidinedione may be pentoxazone. Phenylpyrazole may be selected from the group consisting of fluazolate, piraflufene, and piraflufene-ethyl. Pyrimidinediones may be selected from the group consisting of benzphenidizone, butafenacil, epiripencacil (S-3100), flupropacil, flufenoxymasil, saflufenacil, and thiafenacil. Thiadiazoles may be selected from the group consisting of fluthiacet-methyl and thidiazimine. Triazolinones may be selected from the group consisting of azafenidine, bencarbazone, carpentrazone, their salts and esters, and sulfentrazone. Benzoxazinone derivatives are 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3,It may be 4-dihydro-3-oxo-4-prop-2-inyl-2H-1,4-benzoxazine-6-yl)-1,3,5-triazinan-2,4-dion (trifludimoxazine). Other PPO herbicides include chlorphthalim, flufenpyr, flufenpyr-ethyl, flumipropin, pyraclonil, profluazole, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate; cyclopropylmethyl (2-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate; Methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate(fluphenoxymacil), methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} propanoate, methyl 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate methyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid,(2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, ethyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, methyl (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, ethyl (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-propyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]phenyl}-5-ethyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazole-3-yl)phenyl]-1-methyl-6-(trifluoromethyl)pyrimidine-2,4(1H,3H)-dione, ethyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazole-3-yl)phenyl]-1,5-dimethyl-6-sulfanylidene-1,3,5-triazinan-2,4-dione, ethyl 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid,methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, methyl 3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, 2-ethoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate, It may be selected from the group consisting of {[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}acetic acid, and 2-methoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate.

[0045] In any method described herein comprising the use of an effective amount of PPO herbicide, the effective amount of PPO herbicide may be about 0.0009 lb / acre to about 1.5 lb / acre over the growing season.

[0046] A method for producing offspring soybean plants containing soybean event Gm_CSM63717 is provided. The method comprises: a) sexually crossing a first soybean plant containing soybean event Gm_CSM63717 with itself or a second soybean plant; b) collecting one or more seeds produced by the cross; c) growing one or more seeds to produce one or more offspring plants; and d) selecting at least one first offspring plant or seed containing soybean event Gm_CSM63717. An inbred or hybrid soybean plant or seed containing soybean event Gm_CSM63717 produced by the method is also provided herein.

[0047] Inactive or non-renewable soybean plant material is provided. The inactive or non-renewable soybean plant material comprises any of the recombinant DNA molecules provided herein or any of the DNA constructs provided herein.

[0048] Another non-living or non-renewable soybean plant material is provided. The non-living or non-renewable soybean plant material includes soybean event Gm_CSM63717, which is a representative sample of seeds containing soybean event Gm_CSM63717 deposited under ATCC accession number PTA-127604.

[0049] A commercial product is provided. The commercial product comprises any of the recombinant DNA molecules provided herein or any of the DNA constructs provided herein. The commercial product may be produced from a transgenic soybean plant, plant part, plant seed, or plant cell containing the soybean event Gm_CSM63717. The commercial product comprises whole or processed seeds; viable or non-viable seeds; viable plant parts (e.g., roots, nodes, buds, or leaves); viable plant cells; processed plant parts; processed plant tissues; dehydrated plant tissues; dehydrated plant parts; frozen plant tissues; frozen plant parts; foods for human consumption such as soybean oil, soy milk, soybean flour, soybean kernels, soybean protein, soybean protein concentrate, hydrolyzed vegetable protein, textured soy protein, lecithin, curd, tofu, vegetable soybeans (edamame), mung bean sprouts, soybean film (yuba), roasted soybeans, miso, tempeh, soy sauce, or natto; It may include plant parts processed for animal feed, such as soy meal; soy fiber; biodiesel; bio-composite building materials, such as particleboard, laminated plywood, or wood products; soybean oil-based solvents; soybean oil-based commercial lubricants; soybean ink; soybean candles; soybean crayons; soybean-based hydraulic fluids; or soybean-based foam.

[0050] A method for producing a commercial product is provided. The method comprises the steps of: (a) obtaining a transgenic soybean plant, a plant part, or a plant seed containing the soybean event Gm_CSM63717; and (b) producing a commercial product from the transgenic soybean plant, the plant part, or the plant seed.

[0051] A method for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds is provided. The method comprises the step of growing soybean plants in a crop growing environment that contain a transgenic gene providing resistance to (i) a PPO herbicide and (ii) at least three additional modes of herbicide action, wherein the three additional modes of herbicide action are each different from one another.

[0052] Another method for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds is provided. The method comprises: (a) growing a soybean plant in a crop growing environment comprising any of the DNA constructs described herein, or event Gm_CSM63717 and at least three additional transgenic genes that provide resistance to herbicides having at least three additional modes of herbicide action, each of which is different from each other; and (b) applying at least one herbicide selected from the group consisting of dicamba, glufosinate, 2,4-D, PPO inhibitors, β-triketone HPPD inhibitors, glyphosate, and any combination thereof, wherein the soybean plant is resistant to at least one herbicide.

[0053] Transgenic genes that provide resistance to herbicides having at least three additional modes of herbicide action in any of the methods for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds may exist at a single genomic location within soybean plants.

[0054] In addition, a method for reducing loci for soybean breeding is provided by site-specific insertion of a transgenic gene providing resistance to PPO herbicides at a genomic location within a soybean plant within about 1-6 cM of a locus within the genome of a soybean plant containing a transgenic gene for resistance to at least three additional herbicide modes of action, wherein the three additional herbicide modes of action are each different from each other.

[0055] Any of the soybean plants, plant seeds, plant parts, or plant cells described herein comprising at least one additional transgenic gene for resistance to at least one additional herbicide, and any of the methods for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds or methods for reducing loci for soybean breeding, the additional transgenic gene may be selected from the group consisting of FT_Tv7, dicamba monooxygenase (DMO), phosphinothricin N-acetyltransferase (PAT), triketone deoxygenase (TDO), 5-enolpyrubilshikimate-3-phosphate synthase (EPSPS), and any combination thereof. For example, the FT_Tv7 transgenic gene may comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 31. The DMO transgenic gene may comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 29. The PAT transforming gene may comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 27. The TDO transforming gene may comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 33. The EPSPS transforming gene may comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 35. Additional transforming genes may provide resistance to herbicides having a mode of action selected from the group consisting of glutamine synthase inhibitors, EPSPS inhibitors, β-triketone HPPD inhibitors, synthetic auxins, and any combination thereof. Synthetic auxins may be selected from the group consisting of dicamba, 2,4-D, dichloroprop, mecoprop, 2,4,5-T(2,4,5-trichlorophenoxyacetic acid), and any combination thereof. Glutamine synthase inhibitors may include glufosinates.β-triketone HPPD inhibitors may be selected from the group consisting of mesotrione, benzobicyclon (BBC), tembotrion, sulfotrione, tefuryltrione, and combinations of any of these. Inhibitors of 5-enolpyrubilshikimate-3-phosphate synthase (EPSPS) may include glyphosate.

[0056] Any of the soybean plants, plant seeds, plant parts, or plant cells described herein comprising at least one additional transgenic gene for resistance to at least one additional herbicide, and any of the methods for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds or reducing loci for soybean breeding, the soybean plants, plant seeds, plant parts, or plant cells may further comprise soybean event MON89788 and / or soybean event Gm_CSM63714.

[0057] Any of the soybean plant, plant seed, plant part, or plant cell described herein comprising at least one additional transgenic gene for resistance to at least one additional herbicide, and any of the method for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds or the method for reducing a locus for soybean breeding, wherein the soybean plant, plant seed, plant part, or plant cell comprises a sequence selected from the group consisting of SEQ ID NO. 182; SEQ ID NO. 183; SEQ ID NO. 184; SEQ ID NO. 185; SEQ ID NO. 186; SEQ ID NO. 187; SEQ ID NO. 188; SEQ ID NO. 189; SEQ ID NO. 190; SEQ ID NO. 191; A polynucleotide having a sequence identical to the full length of SEQ ID NO. 182 or the full length of SEQ ID NO. 183 by at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9%; and a recombinant DNA molecule comprising the complete complement of any of the above.

[0058] Any of the soybean plant, plant seed, plant part, or plant cell described herein comprising at least one additional transgenic gene for resistance to at least one additional herbicide, and any of the method for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds or the method for reducing a locus for soybean breeding, wherein the soybean plant, plant seed, plant part, or plant cell is a sequence selected from the group consisting of SEQ ID NO. 192, SEQ ID NO. 193, SEQ ID NO. 194, SEQ ID NO. 195, SEQ ID NO. 196, and SEQ ID NO. 197; A polynucleotide having a sequence identical to the full length of SEQ ID NO. 192 or the full length of SEQ ID NO. 193 by at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9%; and a recombinant DNA molecule comprising the complete complement of any of the above.

[0059] An additional method for controlling or preventing weed growth in a region is provided. The method comprises the steps of sowing soybeans in the region containing event Gm_CSM63717, event Gm_CSM63714 and / or event MON89788, and controlling weeds in the region with no damage to the soybeans or less than 10% damage by applying one or more herbicides selected from the group consisting of PPO herbicides, dicamba, glufosinate, 2,4-D, β-triketone HPPD inhibitors, glyphosate, and any combination thereof. Brief explanation of the drawing

[0060] Figure 1 illustrates the sequence of the soybean event Gm_CSM63717. Horizontal lines correspond to the positions of SEQ ID NOs 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, and 12 for SEQ ID NO. 10. Horizontal arrows (SEQ ID NOs 176, 177, 208, and 209) indicate the approximate positions of exemplary primer pairs that can be used to detect the soybean event Gm_CSM63717. Horizontal lines labeled SEQ ID NOs 178 and 210 indicate the approximate positions of exemplary DNA probes that can be used to detect the soybean event Gm_CSM63717 or wild-type soybean sequences. "RB" indicates Agrobacterium ( Agrobacterium ) refers to the right boundary of T-DNA; "LB" is Agrobacterium Refers to the left boundary of T-DNA. "Promoter" indicates the promoter element; "Leader" indicates the leader (5' UTR) element; "Intron" indicates the intron element; "CTP" indicates the chloroplast transport peptide element; "3' UTR" indicates the 3' UTR; "PPO" indicates the protoporpyrinogen oxidase coding element; and "Lox" indicates the lox recombination site. The horizontal line labeled SEQ ID NO. 13 indicates the relative location or position of the wild-type soybean genome where the transform gene (SEQ ID NO. 9) is inserted. The dashed line indicates 10 of the soybean genome at the insertion site of the transform gene (SEQ ID NO. 9). _ It indicates a nucleotide deletion. Figure 2 shows the soybean event Gm_CSM63717 used to transform and generate the event before and after T-DNA integration, and after Cre-mediated excision of the marker cassette. Agrobacterium This is a schematic representation of the T-DNA cassette in the Ti plasmid. "RB" is Agrobacterium Refers to the right boundary of T-DNA; "LB" is Agrobacterium It refers to the left boundary of T-DNA. aad A", "CRE", "Cas12a", "PPO", and "gRNA" are, respectively aad A represents the selectable marker cassette, Cre recombinase cassette, Cas12a nuclease cassette, protoporpyrinogen oxidase cassette, and gRNA cassette. "Lox" represents the lox recombination site. "5' flank" and "3' flank" represent the 5' and 3' flank soybean genome sequences at the T-DNA integration site, respectively. A: "T-DNA before integration" represents the T-DNA containing aadA, CRE, Cas12a, gRNA, and PPO cassettes prior to transformation; B: "Inserted T-DNA after integration" represents the T-DNA integrated into the soybean genome after transformation. aad A represents T-DNA containing CRE, Cas12a, gRNA, and PPO cassette; C: "Inserted T-DNA after Cre-resection" is aad It represents an integrated T-DNA cassette that leaves two Lox sites, a PPO cassette, and one of the left and right boundaries after excision of A, CRE, Cas12a, and gRNA cassettes. Figure 3 illustrates the approximate schedule for research, testing, and development leading to the selection of the commercial event soybean event Gm_CSM63717. "POC" stands for Proof of Concept; "TFN" stands for Transformation; "GH" stands for Greenhouse; "SA" stands for South America; and "NA" stands for North America. Figure 4 is a schematic representation of the breeding process for producing marker-free soybean events. The "R0 transformant" is aad It is an initial transformation event generated by transformation into a binary transformation vector that is half-zygotic to T-DNA alleles containing A, Cre, Cas12a, gRNA, and a PPO cassette. By auto-pollinating an R0 transformant, R1 It generated generations, in which many offspring were located on the sides of the two Lox sites due to Cre-recombinase excision. aad Lost A, Cre, Cas12a, and gRNA cassette. Half-zygous T-DNA positive, aad A, Cre, Cas12a, and gRNA-negative (also known as no marker) plants were selected and self-pollinated to generate the R2 generation. aad R2 plants homozygous for the inserted T-DNA allele lacking A, CRE, Cas12a, and gRNA cassettes were selected and self-pollinated to generate the R3 generation. The R3 generation plants were self-pollinated to produce pure lines of R4 Gold Standard seeds. R3 generation plants, which were marker-free and homozygous for the inserted T-DNA, were used for inbreeding efficacy and agronomic field trials. Subsequent "R" generations (R1, R2, and R3) represent successive generations produced through self-pollination of plants derived from the initial R0 transgenic plant that generated the soybean event Gm_CSM63717. Figure 5 shows the yield results of seven targeted events of crops GmHT5-1 and GmHT5-2 in the fourth season of agronomic field trials conducted in South America. Yields are expressed as % yield change compared to wild-type controls. Figure 6 shows the yield results for seven targeted events of crops GmHT5-1 and GmHT5-2 in the fourth season efficacy field test conducted in South America. Yields are expressed as % yield change compared to the untreated control group. Herbicide treatments included 1) 448 g / ha of flumioxazine applied pre-emergence (PRE), followed by stages V3 and R1 (upper panel); and 2) 80 g / ha of epirifenacil (rapidicil) applied pre-emergence, followed by stages V3 and R1 (lower panel). Figure 7 shows the damage grades of seven targeted events for crops GmHT5-1 and GmHT5-2 in the fourth season efficacy field test conducted in South America. Damage grades are expressed as % damage compared to untreated controls. Herbicide treatments included 1) 448 g / ha of flumioxazine applied pre-emergence (PRE), followed by stages V3 and R1 (upper panel); and 2) 80 g / ha of epirifenacil (rapidicil) applied pre-emergence, followed by stages V3 and R1 (lower panel). A brief explanation of the sequence SEQ ID NO. 1 is a 30-nucleotide sequence representing the 5' junction region of soybean genomic DNA and an integrated transgenic gene insert. SEQ ID NO. 1 corresponds to nucleotide positions 986-1015 of SEQ ID NO. 10. SEQ ID NO. 2 is a 30-nucleotide sequence representing the 3' junction region of the integrated transgenic gene insert and soybean genomic DNA. SEQ ID NO. 2 corresponds to nucleotide positions 4,187-4,216 of SEQ ID NO. 10. SEQ ID NO. 3 is a 60-nucleotide sequence representing the 5' junction region of soybean genomic DNA and the integrated transgenic gene insert. SEQ ID NO. 3 corresponds to nucleotide positions 971-1030 of SEQ ID NO. 10. SEQ ID NO. 4 is a 60-nucleotide sequence representing the 3' junction region of the integrated transgenic gene insert and soybean genomic DNA. SEQ ID NO. 4 corresponds to nucleotide positions 4,172-4,231 of SEQ ID NO. 10. SEQ ID NO. 5 is a 100-nucleotide sequence representing the 5' junction region of soybean genomic DNA and the integrated transgenic gene insert. SEQ ID NO. 5 corresponds to nucleotide positions 951-1050 of SEQ ID NO. 10. SEQ ID NO. 6 is a 100-nucleotide sequence representing the 3' junction region of the integrated transgenic gene insert and soybean genomic DNA. SEQ ID NO. 6 corresponds to nucleotide positions 4,152-4,251 of SEQ ID NO. 10. SEQ ID NO. 7 is a 1,050-nucleotide sequence representing 50 bp of the 5' genomic lateral region of soybean genome DNA and the integrated transforming gene insert. SEQ ID NO. 7 corresponds to nucleotide positions 1-1,050 of SEQ ID NO. 10. SEQ ID NO. 8 is a 1,050-nucleotide sequence representing a 50 bp 3' junction region of the integrated transgenic gene insert and a 3' genomic lateral region of soybean genomic DNA. SEQ ID NO. 8 corresponds to nucleotide positions 4,152-5,201 of SEQ ID NO. 10. SEQ ID NO. 9 is a 3,201-nucleotide sequence corresponding to the transforming gene insert of the soybean event Gm_CSM63717. SEQ ID NO. 9 corresponds to nucleotide positions 1,001-4,201 of SEQ ID NO. 10. Sequence No. 10 is a contig nucleotide sequence of the 5' soybean genome DNA sequence (Sequence No. 11), a transforming gene insert of event Gm_CSM63717 (Sequence No. 9), and a 5,201-nucleotide sequence corresponding to the 3' soybean genome DNA sequence (Sequence No. 12). SEQ ID NO. 11 is a 1,000-nucleotide sequence representing 5' flank soybean genomic DNA up to the transgenic gene insert (SEQ ID NO. 9). SEQ ID NO. 11 corresponds to nucleotide positions 1-1,000 of SEQ ID NO. 10. SEQ ID NO. 12 is a 1,000-nucleotide sequence representing the 3' flank soybean genomic DNA after the transgenic gene insert (SEQ ID NO. 9). SEQ ID NO. 12 corresponds to nucleotide positions 4,202-5,201 of SEQ ID NO. 10. SEQ ID NO. 13 is a 2,010-nucleotide sequence representing wild-type soybean genomic DNA at the insertion site of the transformation sequence (SEQ ID NO. 9) in event Gm_CSM63717. The 10-nucleotide fragment (nucleotides 1,001-1,010) of SEQ ID NO. 13 was deleted in event Gm_CSM63717 due to the insertion of T-DNA. SEQ ID NO. 14 is a 5,000-nucleotide sequence representing soybean genomic DNA on the side of the transformed insert at the 5' end of the insert. This sequence was based on the genomic sequence of the soybean Williams 82 germplasm. SEQ ID NO. 15 is a 5,000-nucleotide sequence representing soybean genomic DNA on the side of the transformed insert at the 3' end of the insert. This sequence was based on the genomic sequence of the soybean Williams 82 germplasm. Sequence numbers 16-24 are nucleotide sequences for genetic elements in the transforming insert of the soybean event Gm_CSM63717 and are additionally listed in Table 1 below. Sequence number 25 is Enterobacter cloacae This is the amino acid sequence of protoporpyrinogen oxidase (PPO) from. Sequence numbers 26 and 27 are codon-optimized nucleotide and amino acid sequences of the phosphinothricin N-acetyltransferase gene (PAT), respectively. Sequence numbers 28 and 29 are codon-optimized nucleotide and amino acid sequences of the dicamba monooxygenase (DMO) gene, respectively. Sequence numbers 30 and 31 are the codon-optimized nucleotide and amino acid sequences of the FT_Tv7 gene, respectively. Sequence numbers 32 and 33 are codon-optimized nucleotide and amino acid sequences of the triketone deoxygenase (TDO) gene, respectively. Sequence numbers 34 and 35 are codon-optimized nucleotide and amino acid sequences of the 5-enolpyrubilchimate-3-phosphate synthase (EPSPS) gene, respectively. SEQ ID NOs 36-105 are 5' side genome sequences within the 5' side genome sequence of event Gm_CSM63717. SEQ ID NOs 36-55 are based on the genome sequence of the transformed germplasm; SEQ ID NOs 56-105 are based on the genome sequence of the soybean Williams 82 germplasm. SEQ ID NOs 106-175 are 50-nucleotide sequences within the 3' side genomic sequence of event Gm_CSM63717. SEQ ID NOs 106-125 are based on the genomic sequence of the transformed germplasm; SEQ ID NOs 126-175 are based on the genomic sequence of the soybean Williams 82 germplasm. SEQ ID NO 176 is a 22-nucleotide sequence corresponding to the heat amplification primer referred to as SQ51702 used in event-specific and compatibility assays for detecting soybean event Gm_CSM63717 DNA in a sample, and is identical to the reverse complement of the nucleotide sequence corresponding to positions 1,068-1,089 of SEQ ID NO 10. SEQ ID NO 177 is a 32-nucleotide sequence corresponding to the heat amplification primer referred to as SQ52020 used in event-specific and compatibility assays for detecting soybean event Gm_CSM63717 DNA in a sample, and is identical to the nucleotide sequence corresponding to positions 931-962 of SEQ ID NO 10. SEQ ID NO. 178 is an 18-nucleotide sequence corresponding to the 6FAM-MGB probe referred to as PB50308 used in event-specific and compatibility assays for detecting soybean event Gm_CSM63717 DNA in a sample, and is identical to the reverse complement of the nucleotide sequence corresponding to positions 1,045-1,062 of SEQ ID NO. 10. Sequence No. 179 is a 20-nucleotide sequence corresponding to the heat amplification primer referred to as SQ546, which is used as an internal control for event testing for the soybean event Gm_CSM63717 and hybridizes to the soybean genome region. Sequence No. 180 is a 20-nucleotide sequence corresponding to the heat amplification primer referred to as SQ549, which is used as an internal control for event testing for the soybean event Gm_CSM63717 and hybridizes to the soybean genome region. Sequence No. 181 is an 18-nucleotide sequence corresponding to the VIC-MGB probe referred to as PB50207, which is used as an internal control for event testing for the soybean event Gm_CSM63717 and hybridizes to the soybean genome region. Sequence No. 182 is the nucleotide sequence of the soybean event Gm_CSM63714 corresponding to the contig nucleotide sequences of the 5' flank soybean genome sequence, the transform insert, and the 3' flank soybean genome sequence. Sequence number 183 is the nucleotide sequence of the transform insert of the soybean event Gm_CSM63714. Sequence numbers 184-187 are the 5' junction sequences of the soybean event Gm_CSM63714. Sequence numbers 188-191 are the 3' junction sequences of the soybean event Gm_CSM63714. Sequence No. 192 is the 6466-nucleotide sequence of the soybean event MON89788 corresponding to the contig nucleotide sequences of the 5' flank soybean genome sequence, the transform insert, and the 3' flank soybean genome sequence. Sequence No. 193 is the nucleotide sequence of the transform insert of soybean event MON89788 (nucleotides 1104-5409 of sequence No. 9 of US7632985). Sequence numbers 194-195 are the 5' junction sequences of the soybean event MON89788. Sequence numbers 196-197 are the 3' junction sequences of the soybean event MON89788. Sequence numbers 198 and 199 are, respectively Lachnospiraceae bacteria This is the nucleotide and amino acid sequence of LbCas12a (also known as LbCpf1) of ND2006. Sequence number 200 is the amino acid sequence for LbCas12a_V1(G532R / K595R). Sequence No. 201 is the amino acid sequence for LbCas12a_V2(G532R / K538V / Y542R). Sequence number 202 is Francisela_Novicida( Francisella_novicida This is the amino acid sequence for Cas12a (FnCas12a) of ). Sequence No. 203 is a nucleotide sequence for the gRNA repeat portion of LbCas12a. Sequence No. 204 is a nucleotide sequence for the gRNA repeat portion of FnCas12a. Sequence number 205 is a nucleotide sequence for gRNA gRNA_5F-134. Sequence number 206 is the nucleotide sequence for gRNA gRNA_3F-15. Sequence No. 207 is a 21-nucleotide downstream mature crRNA scaffold sequence. SEQ ID NO 208 is a 30-nucleotide sequence corresponding to the heat-amplifying forward primer referred to as SQ52185 used in a compatibility assay for the detection of wild-type (WT) allele DNA in a sample, and hybridizes to the soybean genome region. This is identical to the inverse complement of the nucleotide sequence corresponding to positions 4,220-4,249 of SEQ ID NO 10. SEQ ID NO 209 is a 27-nucleotide sequence corresponding to the heat amplification primer referred to as SQ52186 used in the fusion assay for the detection of WT allele DNA in a sample, and hybridizes to the soybean genome region. This corresponds to positions 941-967 of SEQ ID NO 10. SEQ ID NO 210 is an 18-nucleotide sequence corresponding to the VIC-MGB probe referred to as PB50725 used in a fusion assay for the detection of WT allele DNA in a sample, and hybridizes to a soybean genome region. This corresponds to positions 4,201-4,218 of SEQ ID NO 10. Sequence numbers 211-222 are nucleotide sequences of gRNA target sites. Sequence No. 223-234 is the nucleotide sequence of the gRNA spacer sequence corresponding to Sequence No. 211-222. Sequence No. 235 is the nucleotide sequence of the nuclear localization signal (NLS) from tomato heat stress transcription factor HSFA1. Sequence No. 236 is the nucleotide sequence of a plant codon-optimized sequence encoding LbCas12a used in a gRNA test. Sequence number 237 is Medicago Trunkatula It is a 3' UTR sequence derived from a gene. Sequence No. 238 is the nucleotide sequence of the enhancer region + promoter of the banana striae virus strain Acuminata ba'vi's and the 5' UTR region of the Daria mosaic virus. Sequence No. 239 is a 20-nucleotide direct repeat or mature crRNA scaffold sequence. Specific details for implementing the invention

[0061] The following definitions, descriptions, and methods are provided to better define the invention and to guide those skilled in the art in practicing the invention. Unless otherwise specified, terms should be understood according to the common usage of those skilled in the relevant art.

[0062] Herbicide resistance is an important agronomic trait for effective weed control to maintain favorable crop growing conditions and crop yields, and is achieved by manipulating herbicide resistance transgenic genes in crop plants using modern plant biotechnology. The soybean event Gm_CSM63717 confers resistance to PPO herbicides and provides another mode of action for weed control and the management of herbicide-resistant weeds.

[0063] Soybean event Gm_CSM63717 is provided. Event Gm_CSM63717 is a soybean seed-derived embryo ex-situ convenience having a DNA construct containing five transgenic gene cassettes. Agrobacterium It is produced by mediated transformation. The first cassette is designed to confer resistance to PPO herbicides. Enterobacter cloacae It encoded protoporpyrinogen oxidase (PPO). The second cassette is a selectable marker for the selection of transformation events. E. coli Tn7 aminoglycoside-3'-adenyltransferase ( aad A) was encoded. The third cassette is for the integration of site designation of the transformed gene. Lachnospiraceae bacterium It encoded a codon-optimized Cas12a nuclease from ND2006 (LbCas12a or LbCpf1). The fourth cassette encoded a gRNA to direct the Cas12a nuclease to its soybean genome target region. The fifth cassette is Esherikia The Cre recombinase of virus P1 was encoded. aad There are two lox sites on the sides of A, Cas12a, Cre, and gRNA cassettes, and they were removed during Cre enzyme expression.

[0064] AgrobacteriumPlant transformation technologies, such as mediation or gene gun transformation, can be used to produce genetically engineered plant cells, also referred to as "transformed" or "recombined" cells, by inserting foreign DNA (also known as transforming DNA) into chromosomes within plant cells either randomly or via site-specific insertions (also known as targeted insertions). Using these transformation technologies, many individual cells can be transformed, each resulting in a unique "transformation event" or "event" by inserting foreign DNA into the genome. Transformed plants can then be regenerated from each individual transformed cell. This results in all cells of the transformed plant containing the uniquely inserted transformation event as a stable part of the genome. The transformed plants can then be used to produce offspring plants, each containing a unique transformation event. The term "transformation" refers to a plant, plant part, plant cell, seed, offspring plant, or DNA molecule, construct, or sequence containing a transforming gene. for example "Transformed cell" refers to a cell containing a transformed gene.

[0065] The soybean event Gm_CSM63717 was produced and identified through a complex research and development process. This process involved (i) the design and selection of a DNA construct containing five transgenic gene cassettes based on the design and testing of individual transgenic gene cassettes with combinations of different expression factors; (ii) the identification and screening of different transgenic gene target sites, followed by the design and testing of different guide RNAs for efficient cleavage at the target sites; (iii) the transfection of thousands of soybean cells into the DNA construct; (iv) the reproduction of a large population of transgenic events; and (v) rigorous multi-year selection of constructs and events involving the molecular characterization of a large number of transgenic events, greenhouse and field trials for herbicide resistance efficacy and agronomic performance in different locations and geographical regions. Thus, the soybean event Gm_CSM63717 was produced and selected as a uniquely superior event useful for agronomic commercial purposes on a wide scale. Figure 3 illustrates a rough schedule for research, testing, and development leading to the selection of the commercial event soybean event Gm_CSM63717.

[0066] Detailed molecular characterization was performed on the transformation event. Event Gm_CSM63717 was selected based on strict molecular criteria as well as other selection criteria such as herbicide resistance efficacy and agronomic performance. The results of these molecular analyses confirmed the following: (1) Event Gm_CSM63717 contains a single T-DNA inserted at a targeted location containing a single copy of the transformation insert containing only the PPO cassette; (2) a single lox site between the left and right boundaries of the PPO expression cassette and the T-DNA, e.g., the transformation insert backbone sequence or aadNo additional elements of the transformation construct other than A, Cas12a, CRE, and gRNA cassettes or fragments were present; (3) the transformation DNA was inserted into an intergene region far removed from any endogenous gene or repeat region; and (4) the transformation event produced transcripts and proteins of the correct size for the PPO transformation gene by northern hybridization and western hybridization analysis, respectively. Furthermore, DNA sequencing and protein expression assays were performed to (1) determine the 5' and 3' transformation insert-plant genome junctions; (2) confirm the organization of elements within the insert; (3) verify the complete nucleotide sequence of the inserted transformation DNA (Sequence No. 9); and (4) determine PPO protein levels in different tissues such as leaves, roots, and seeds, and PPO protein levels in leaves over multiple generations. In addition, primers and probes were designed, and a heat amplification assay was developed and validated to produce a specific amplicon diagnosis for the presence of event Gm_CSM63717 in samples. As used herein, regarding the junction, orientation, and site of the transformation event insertion, the 5' and 3' designations are relative to the right boundary of the inserted T-DNA in the direction of the left boundary, where the 5' junction and genomic sequence are upstream of the transformation gene at the right boundary, and the 3' junction and genomic sequence are downstream of the transformation gene at the left boundary.

[0067] As used herein, "expression cassette" or "cassette" or "transformation gene cassette" is a recombinant DNA molecule or sequence comprising a combination of distinct elements for expressing RNA and / or proteins encoded by the coding sequence of a transformation gene in a transformed plant cell or in a transformed plant containing the transformation gene. As provided herein, "expression cassette" or "cassette" or "transformation gene cassette" comprises one or more regulatory element(s) operably linked to a coding or transcriptional DNA sequence. The regulatory element may include a promoter, a reader, a 5' untranslating region (5' UTR), an intron, and / or a 3' untranslating region (3' UTR). The "expression cassette" or "cassette" or "transformation gene cassette" is recombinant and heterogeneous with respect to the combination of different genetic elements as well as the transformed plant cell genome. For the purposes of the present disclosure, such “expression cassette” or “cassette” or “transformed gene cassette” is a recombinant DNA molecule or sequence encoding a protein for conferring resistance to at least one class of herbicides as described herein. Table 1 provides a list of genetic elements contained in the transformed gene cassette in the transformed insert of the soybean event Gm_CSM63717 (SEQ No. 9).

[0068] The insertion of transformed DNA into the genome of a soybean plant is achieved by a plant transformation method known in the art and generates a new transformed genomic DNA sequence known as a “transformation event,” “event,” or “transformation event locus.” The DNA sequence of the event consists of the inserted foreign DNA (referred to as the “transformation insert”) and genomic DNA adjacent to or “lateral” to the transformation insert on both sides of the insertion site. As used herein with respect to the transformation event, the term “lateral” refers to plant genomic sequence(s) adjacent to the transformed DNA insert in the genome of a transformed plant, plant part, plant tissue, or plant cell containing the transformation event at the 5’ and / or 3’ end(s) of the transformation event insert. Likewise, “lateral DNA” refers to the length of the genomic DNA sequence adjacent to the transformed DNA insert of the transformed event within the genome at the 5’ and / or 3’ end(s) of the insert. Accordingly, the "5' side" refers to a soybean genomic DNA sequence adjacent to and upstream (or at the 5' end) of the transformed DNA insert. For example, the "5' side" may include a soybean genomic DNA sequence immediately adjacent to and upstream (at the 5' end) of the transformed insert, or any soybean genomic DNA sequence that is not immediately adjacent to the transformed insert but is upstream (at the 5' end) of the transformed insert within about 5,000 nucleotides, within about 3,000 nucleotides, or within about 1,000 nucleotides. Likewise, the "3' side" refers to a soybean genomic DNA sequence adjacent to and downstream (or at the 3' end) of the transformed insert.For example, the “3’ side” may include a soybean genome DNA sequence immediately adjacent to and downstream (3’ end) of the transformation insert, or any soybean genome DNA sequence downstream (3’ end) of the transformation insert that is not immediately adjacent to the transformation insert but is downstream of the transformation insert within about 5,000 nucleotides, within about 3,000 nucleotides, or within about 1,000 nucleotides. The DNA sequence of the event is unique and specific to the event and can be easily identified when compared with other DNA sequences, such as other events or untransformed soybean genome DNA. The soybean event Gm_CSM63717 is a novel and unique DNA sequence provided as SEQ ID NO. 10 and includes adjacent sequences including the 5’ soybean genome side sequence provided as SEQ ID NO. 11, the transformation insert sequence provided as SEQ ID NO. 9, and the 3’ soybean genome side sequence provided as SEQ ID NO. 12 (Fig. 1). Therefore, the soybean event Gm_CSM63717 is a DNA molecule that is an essential part of the chromosomes of the transgenic soybean cells and plants containing the event, and thus can be transmitted statically to progeny cells and plants. As further described in the examples below, various gene editing tools exist that allow modification of the transgenic inserts of the soybean event Gm_CSM63717 and / or lateral genomic DNA, such as by deletion, insertion, translocation, or substitution of nucleic acid sequence(s), and the event uniquely characterizes the presence of heterologous DNA at specific locations of the genome occupied by the soybean event Gm_CSM63717 in relation to lateral locations of the native soybean genome.

[0069]

[0070]

[0071]

[0072] The offspring of the original transformed cells and plants containing the soybean event Gm_CSM63717 are provided. These offspring may be produced by selfing of the soybean plant containing the soybean event Gm_CSM63717, or by sexual cross or outcrossing between the soybean plant containing the soybean event Gm_CSM63717 and another plant containing or not containing the event, or by any other method known in the art, including any plant cell or tissue culture method, wherein the offspring contain the soybean event Gm_CSM63717. The other plant may be a transformed plant containing the same and / or different event(s) or a non-transformed plant, and each parent plant of the cross or outcross may be the same or different germplasm or breeding line. The soybean event Gm_CSM63717 is transmitted from the original parents to the offspring through each generation. Accordingly, the "transformed plant" or "plant" may be the original transformant plant regenerated from the transformed plant cell and containing the transform DNA and events, or a descendant plant of the original transformant plant that can be separated from the transformant by one or more generations maintaining the transform DNA and events in the same specific location and sequence context in the plant's genome. The transformant or descendant plant may be homozygous or heterozygous for the event Gm_CSM63717. Furthermore, the "transformed plant" is a plant having a transform gene stably inserted into the genome of at least one cell of the plant ( in other words , at least one cell of the plant may contain the soybean event Gm_CSM63717), and the plant may be chimeric or non-chimeric with respect to the transforming gene and / or event. The transformed plant is chimeric with respect to the transforming gene if not all cells of the plant contain the transforming gene.

[0073] Since the present disclosure describes the introduction of event Gm_CSM63717 into soybeans, the term "soybean event Gm_CSM63717" is used herein to refer to the event. However, those skilled in the art will know that event Gm_CSM63717 Glycine soja You will understand that it can be introduced into other varieties or related soybean species through crosses such as.

[0074] The soybean event Gm_CSM63717 provides soybean cells, plants, plant parts, seeds, and offspring containing event resistance to PPO herbicides. The terms "PPO herbicide," "PPO inhibitor," and "PPO-inhibitory herbicide" are used interchangeably herein and refer to chemotherapy agents that target and inhibit the enzymatic activity of protoporpyrinogen oxidase (PPO). Soybean Event Gm_CSM63717 is flumioxazine, epirifenacil (also referred to as S-3100 or lapidicil; IUPAC name: ethyl [(3-{2-chloro-5-[3,6-dihydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)pyrimidine-1(2H)-yl]-4-fluorophenoxy}-2-pyridyl)oxy]acetate), lactophen, asifluorophen, pyraflufen, pyraflufen-ethyl, oxadiazone, butafenacil, 피리딘-2-일메틸 [(3-{ 2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-시아노-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 시아노메틸 [(3-{2-브로모-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 사이클로프로필메틸 (2-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}페녹시)아세테이트, It provides resistance to various PPO herbicides including but not limited to methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoxymasil), fomesafene, ciflufenacil, sulfentrazone, tiafenacil, and trifludimoxazine.

[0075] The soybean event Gm_CSM63717 inserts a single copy into a single site-designated locus of the soybean genome, thereby creating two new locus or junction sequences across the inserted DNA and a portion of the soybean genomic DNA not known to naturally appear or exist in the soybean genome or other transformed soybean events ( for example Characterized by generating the sequences presented in Sequence No. 1, Sequence No. 2, Sequence No. 3, Sequence No. 4, Sequence No. 5, Sequence No. 6, Sequence No. 7, and Sequence No. 8, and in other words These are unique to event Gm_CSM63717. Sequence No. 1, Sequence No. 3, Sequence No. 5, and Sequence No. 7 span the 5' junction and transformed DNA insertion of the soybean genome sequence, and Sequence No. 2, Sequence No. 4, Sequence No. 6, and Sequence No. 8 span the 3' junction. These junction sequences are useful for detecting the presence of event Gm_CSM63717 in plant products such as soybean cells, seeds, plants, plant parts, offspring, and soybean commercial products. Polynucleotide or DNA molecular probes and / or primer pairs are described herein for use in identifying the presence of these various junction sequences in biological samples containing, derived from, or suspected of containing, soybean cells, seeds, plants, plant parts, offspring, or commercial products containing event Gm_CSM63717.

[0076] As used herein, with respect to a specific DNA molecule, amplicon, or sequence in connection with a soybean plant, plant part, seed, offspring, cell, and / or soybean plant product, e.g., commercial product, the term “derived from” or “derived therefrom” means that the DNA molecule, amplicon, or sequence is taken directly or indirectly from, purified, isolated, or manufactured from such soybean plant, plant part, seed, offspring, cell, and / or soybean plant product, e.g., commercial product. Alternatively, with respect to a soybean plant, plant part, seed, offspring, or cell, the term “derived from” or “derived therefrom” in connection with a soybean plant, plant part, seed, offspring, or cell, e.g., commercial product, means that the soybean plant product is taken directly or indirectly from, purified, isolated, or manufactured from such soybean plant, plant part, seed, offspring, or cell.

[0077] "Detectable" refers to the ability of a specific DNA molecule, segment, or sequence to be detected in a sample, for example, by amplification by DNA sequencing and determination of its presence, size, or sequence, and / or binding of a probe to a target DNA molecule, segment, or sequence.

[0078] "Sample" is intended to refer to any composition containing or derived directly or indirectly from a biological sample, source, or material. The sample may generally contain soybean DNA and / or soybean DNA that is substantially or completely pure, purified, or isolated. "Biological sample" contains biological material containing, but not limited to, DNA obtained directly or indirectly from or derived from the genomes of soybean cell(s), tissue(s), seed(s), plant(s), plant part(s), and / or soybean plant product(s), such as commercial product(s). Such soybean cell(s), tissue(s), seed(s), plant(s), plant part(s), and / or soybean plant product(s), such as commercial product(s), may contain soybean event Gm_CSM63717, or DNA molecule(s) and / or DNA segment(s) containing soybean event Gm_CSM63717. In some embodiments, the sample or biological sample may comprise soybean cell(s), soybean tissue(s), soybean seed(s), soybean plant(s), soybean plant part(s) and / or soybean plant product(s), and its cell or cell membrane may be crushed to release the contents of the soybean cell(s) containing genomic DNA or protein and / or to make the contents of the soybean cell(s) containing genomic DNA or protein accessible or usable for testing or examination ( for example ..., destruction or opening) was performed. "Directly" refers to directly obtaining DNA from the soybean genome by a person skilled in the art by lysing soybean cells (or obtaining a soybean sample containing lysed soybean cells) and exposing or using the genomic DNA or proteins of the soybean cells for detection purposes. "Indirectly" refers to obtaining target or specific reference DNA ( for example, refers to obtaining the novel and unique splicing segment(s) described herein by a person skilled in the art as a diagnosis of the presence of event Gm_CSM63717. Such indirect means include the amplification of a DNA segment containing a DNA sequence targeted by specific probe(s) and / or primer set(s) designed to bind specifically to or near the target sequence, or the amplification of a DNA segment containing all or part of a target sequence that can be measured or characterized ( for example ...is characterized by being measured by transfer or separation from other segments of DNA and / or identification in an effective matrix, such as agarose or acrylamide gel, direct sequencing of the amplicon(s), or cloning the amplicon(s) into the vector(s) and direct sequencing of the inserted amplicon(s) present in the vector(s).

[0079] As used herein, the term “recombinant” refers to non-naturally occurring DNA, proteins, combinations, or organisms that are not generally found or exist in nature and are produced by human intervention. As used herein, “recombinant DNA molecule” is a DNA molecule comprising a combination of DNA molecules that do not occur together naturally and are the result of human intervention. Two or more elements of such combination of DNA sequences may be operably linked to each other. For example, a recombinant DNA molecule may comprise a combination of at least two DNA molecules that are heterogeneous to each other, such as a coding sequence operably linked to a heterogeneous promoter and / or other regulatory expression element(s), and / or a DNA molecule comprising a transgenic gene and heterogeneous plant genomic DNA adjacent to the transgenic gene, and / or a DNA molecule comprising a polynucleotide sequence that is artificially synthesized and derived from any polynucleotide sequence that is generally found in nature. The recombinant DNA molecule may include all or part of the junction sequence of the event genome and all or part of the transform insert of the event genome, or may include a recombinant or heterologous DNA fragment of the soybean event Gm_CSM63717. Examples of recombinant DNA molecules include at least one polynucleotide sequence selected from the group consisting of SEQ NO 1, SEQ NO 2, SEQ NO 3, SEQ NO 4, SEQ NO 5, SEQ NO 6, SEQ NO 7, SEQ NO 8, SEQ NO 9 and SEQ NO 10, the full length of SEQ NO 10 or the full length of SEQ NO 9 and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.It comprises a DNA molecule comprising a polynucleotide having 9% identical nucleotide sequences and the complete complement of any of the foregoing. Such recombinant DNA molecules may be derived from soybean plants, seeds, plant parts, plant cells, offspring plants, or commercial products containing soybean event Gm_CSM63717. Alternatively, such recombinant DNA molecules may be contained in soybean plants, seeds, plant parts, plant cells, or offspring plants containing soybean event Gm_CSM63717, or commercial products produced therefrom. A representative sample of seeds containing soybean event Gm_CSM63717 was deposited under ATCC accession number PTA-127604. Such recombinant DNA molecules may be formed by inserting a heterologous nucleic acid molecule into the genomic DNA of soybean plants or soybean cells. Such recombinant DNA molecules may be an amplicon diagnostic for the presence of soybean event Gm_CSM63717.

[0080] As used herein, with respect to a plant, plant part, seed, plant cell, or offspring, “recombination” is a plant, plant part, seed, plant cell, or offspring containing a transformed DNA molecule that is not generally found in nature, is the result of human intervention, and is stably integrated into the genome of the plant, plant part, seed, plant cell, or offspring. As a result of such genomic insertion, the recombinant or transformed plant, plant part, seed, plant cell, or offspring is distinctly different and novel from any associated wild-type or naturally occurring plant, plant part, seed, plant cell, or offspring. An example of a recombinant plant is a soybean plant containing the soybean event Gm_CSM63717.

[0081] As used herein, the term “transformed gene” refers to a DNA molecule artificially incorporated into the genome of an organism as a result of human intervention, such as by plant transformation methods. The transformed gene may be heterologous to the organism. As used herein, the term “transformed insert” refers to foreign or heterologous DNA inserted into the soybean genome by plant transformation technology to produce the soybean event Gm_CSM63717. The sequence for the transformed insert of the soybean event Gm_CSM63717 is provided at SEQ ID NO. 9.

[0082] As used herein, with respect to a combination of two or more DNA sequences or elements, the term “heterogeneous” means that two or more DNA sequences or elements generally do not exist together in such combination in nature without human intervention. For example, a DNA molecule may be derived from a first species or recombinant DNA molecule and inserted into the genome of a second species. Thus, the DNA molecule would be heterogeneous with respect to the genome and the organism. As used herein, with respect to a DNA molecule, composition, sequence, or protein in relation to a plant, microorganism, plant cell, or plant genome, the term “heterogeneous” means that the DNA molecule, composition, sequence, or protein does not exist in nature as part of such plant, microorganism, plant cell, or plant genome, or / or does not exist in nature as part of such plant, microorganism, plant cell, or plant genome in the same physical or genomic location, context, or orientation without human intervention.

[0083] As used herein, the term “chimera” refers to a single DNA molecule produced by fusing a first DNA molecule with a second DNA molecule, wherein neither the first DNA molecule nor the second DNA molecule will generally be found in the corresponding configuration in which they are fused together. Thus, a chimeric DNA molecule is a novel DNA molecule not generally found in nature. An example of a chimeric DNA molecule is a DNA molecule comprising at least one sequence selected from SEQ ID NOs 1-10.

[0084] As used herein, the term “isolated” with respect to a molecule means that the molecule is at least partially isolated from other molecules that are generally associated in the native or natural state. In some embodiments, the term “isolated” refers to a DNA molecule that is at least partially isolated from nucleic acids or polynucleotides or DNA sequence(s) that are generally adjacent to and covalently linked to the sequence of the DNA molecule in the native or natural state. A “isolated” DNA molecule may have a DNA sequence corresponding to a part of the plant cell genome without other genomic DNA sequence(s) that are generally adjacent to and covalently linked to the DNA sequence in nature. Such a “isolated” DNA molecule may include all or part of the soybean event Gm_CSM63717 or all or part of a transforming gene and / or transforming event that may include the transforming gene or expression cassette described herein. Nucleic acid sequences or elements, such as coding sequences, intron sequences, 5’ UTRs, promoter sequences, 3’ UTRs, etc., that are naturally found within the DNA of an organism’s genome are not considered “isolated” insofar as the element is located within the organism’s genome and within the location of the genome where it is naturally found. However, each of these elements and sub-parts of these elements will be “isolated” within the scope of this disclosure unless the element or sub-part is located within the genome of an organism and within a location within the genome of an organism found naturally. The “isolated” DNA molecule may be any recombinant DNA molecule, amplification product, or amplicon, and / or may comprise any DNA sequence removed from a natural or biological state and covalently fused to another DNA molecule or sequence not associated in nature. Such isolated DNA molecules may be produced using biotechnological techniques, such as manufacturing recombinant DNA or incorporating foreign or heterologous DNA molecules into the chromosomes of cells, plants, or seeds.Accordingly, any DNA molecule containing a transform, recombinant, chimeric, or artificial nucleotide sequence, transform gene, or expression cassette will be considered a “isolated” DNA molecule because such sequences do not occur naturally, regardless of whether the sequence, transform gene, or expression cassette is present in a plasmid, vector, or construct used to transform plant cells, or in the genome of a plant, plant part, plant tissue, plant cell, or offspring, or in detectable amounts in tissues, offspring, biological samples, or commercial products derived from a plant, plant part, plant tissue, offspring, or plant cell. Accordingly, any recombinant DNA molecule or sequence containing all or part of the transform gene or junction sequence of the soybean event Gm_CSM63717, or any fragment derived therefrom, will also be considered “isolated.” The “isolated” DNA molecule may be extracted or purified from transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or may be present in a homogenate, extract, or lysate from any of these transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or may be produced as an amplicon or amplification product from plant genomic DNA and / or DNA extracted or purified from transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or as a homogenate, extract, or lysate from plant(s), plant part(s), plant cell(s) and / or tissue(s). For the purposes of this disclosure, any transgenic polynucleotide or DNA sequence, in other wordsNucleotide sequences of DNA that are inserted into the genome of a plant or bacterium, or that exist in an extrachromosomal vector, will be considered "isolated" nucleotides or DNA sequences, whether they exist within a plasmid or similar structure used to transform cells, within the genome of a plant or bacterium, or in detectable amounts in tissues, offspring, biological samples, or commercial products derived from plants or bacteria. "Isolated" DNA molecules are chemical or biochemical molecules, regardless of whether the molecule is referred to as nucleic acid, nucleic acid sequence, polynucleotide sequence, DNA sequence, nucleic acid molecule, polynucleotide molecule, DNA molecule, etc. "Isolated" molecules may provide commercial applicability whether they exist in plant cells or the plant genome, or outside of plant cells; thus, they provide and demonstrate (and are intended to provide and demonstrate) utility regardless of where the molecule is located.

[0085] As used herein, the terms "corresponding" or "corresponding," etc. refer to the reference polynucleotide sequence ( for example Any given polynucleotide associated with , SEQ ID NO 10 ( for example When used in the context of nucleotide positions, mutations, insertions, and / or substitutions in SEQ ID NO. 9), if a given polynucleotide is aligned to a reference polynucleotide sequence using a global or local sequence alignment algorithm, it refers to the position(s) of polynucleotide residue(s) within a given sequence that have identity with respect to residue(s) in the reference nucleotide sequence.

[0086] DNA molecules, fragments, and their corresponding DNA sequences, as well as detection methods, are provided. As used herein, the terms “DNA,” “DNA molecule,” and “nucleic acid molecule” refer to deoxyribonucleic acid (DNA) molecules. DNA molecules may be of genomic or synthetic origin and / or include recombinant or heterogeneous DNA molecules or sequences. DNA molecules may, by convention, be described from the 5’ (upstream) end to the 3’ (downstream) end. As used herein, the term “DNA sequence” refers to a polynucleotide sequence of a DNA molecule, in other words It refers to a continuous sequence of nucleotides of a DNA molecule. As used herein with respect to polynucleotides or nucleotides of a DNA sequence or molecule, the terms “continuous” and “contiguous” are interchangeable and synonyms and refer to the 5’ to 3’ order of nucleotides in a polynucleotide or DNA sequence, strand, or molecule without any gaps or interruptions. The nomenclature used is required by Part 37 of § 1.822 of the United States Code of Federal Regulations and is set forth in Tables 1 and 3 of Appendix 2 of WIPO Standard ST.25 (1998). By convention, DNA sequences and fragments thereof are disclosed by reference in the 5’ to 3’ direction of only one of the two complementary DNA sequence strands of a DNA molecule. By implication and intent, the sequences of complementary strands provided herein (sequences of complementary strands) are also referred to in the art as inverse complementary or inverse complement sequences, and are expressly intended to be within the scope of this disclosure and within the scope of the claimed subject matter. As used herein, references to SEQ ID NOs. 1-10 and fragments thereof include and refer to the sequences of complementary strands and fragments thereof.

[0087] In addition, a nucleic acid molecule comprising a polynucleotide having a sequence identical to any one of SEQ ID NOs 1-10 in terms of full length and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% is provided.

[0088] For example, a nucleic acid molecule comprising a polynucleotide having a sequence identical to the full length of SEQ ID NO. 10 or the full length of SEQ ID NO. 9 by at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% is provided.

[0089] DNA molecules, or fragments derived therefrom, may also be produced as amplicons of DNA extracted, purified, or isolated from plants, plant parts, seeds, offspring, or plant cells, homogenates, extracts, or lysates of plants, plant parts, plant cells, or seeds or offspring, or from plant parts, plant cells and / or tissues, offspring, or homogenates, extracts, or lysates of plants, plant parts, plant cells, offspring, and / or seeds, which may further comprise the soybean event Gm_CSM63717.

[0090] As used herein, the term “percent sequence identity” or “% sequence identity” refers to the percentage of identical nucleotides or amino acids in the linear polynucleotide or polypeptide sequence of the reference (“query”) sequence (or its complementary strand) compared to the test (“target”) sequence (or its complementary strand) when two sequences are optimally aligned (where appropriate nucleotide or amino acid insertions, deletions, or gaps are less than 20% of the total of the reference sequence for the comparison window). Optimal alignment of sequences for aligning comparison windows is well known to those skilled in the art and is supported by tools such as Smith and Waterman’s local homology algorithm, Needleman and Wunsch’s homology alignment algorithm, Pearson and Lipman’s similarity search method, and, for example, GCG® Wisconsin Package® (Accelrys Inc., San Diego, California), MEGAlign (DNAStar Inc., 1228 S. Park Street, Madison, Wisconsin 53715), and MUSCLE (version 3.6) (Edgar, "MUSCLE: multiple sequence alignment with high accuracy and high throughput" Nucleic Acids ResearchThis can be performed by computerized implementations of such algorithms, such as GAP, BESTFIT, FASTA, and TFASTA, which are available as part of the sequencing software package of 32(5):1792-7(2004)). The “fraction of identity” for the aligned segments of the test sequence and the reference sequence is calculated by dividing the number of identical components shared by the two aligned sequences by the total number of components in the part of the aligned reference sequence segment, i.e., the entire reference sequence or that small defined part of the reference sequence. Percent sequence identity is expressed as the fraction of identity multiplied by 100. Comparisons of one or more sequences may be performed on the full-length sequence, a part thereof, or a longer sequence. Soybean plants, offspring, seeds, cells, plant parts, and commercial products comprising a detectable amount of a polynucleotide having a nucleotide sequence identical to the whole length of SEQ ID NO. 10 or the whole length of SEQ ID NO. 9 by at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% are within the scope of the present disclosure.

[0091] As used herein, the term “fragment” refers to a smaller piece or sequence of a longer or whole DNA molecule or sequence. For example, any one fragment of SEQ ID NOs 1-12 and SEQ ID NOs 14-15 is longer, or at least about 10 consecutive nucleotides, at least about 11 consecutive nucleotides, at least about 12 consecutive nucleotides, at least about 13 consecutive nucleotides, at least about 14 consecutive nucleotides, at least about 15 consecutive nucleotides, at least about 16 consecutive nucleotides, at least about 17 consecutive nucleotides, at least about 18 consecutive nucleotides, at least about 19 consecutive nucleotides, at least about 20 consecutive nucleotides, at least about 21 consecutive nucleotides, at least about 22 consecutive nucleotides, at least about 23 consecutive nucleotides, at least about 24 consecutive nucleotides, at least about 25 consecutive nucleotides, at least about 30 consecutive nucleotides, at least about 35 consecutive nucleotides, at least about 40 consecutive nucleotides, at least It may include a sequence of about 45 consecutive nucleotides, at least about 50 consecutive nucleotides, at least about 60 consecutive nucleotides, at least about 70 consecutive nucleotides, at least about 80 consecutive nucleotides, at least about 90 consecutive nucleotides, at least about 100 consecutive nucleotides, at least about 150 consecutive nucleotides, at least about 200 consecutive nucleotides, at least about 250 consecutive nucleotides, at least about 300 consecutive nucleotides, at least about 400 consecutive nucleotides, or at least about 500 consecutive nucleotides.

[0092] For example, the "fragments" of the transform insert sequence (SEQ No. 9) of the soybean event Gm_CSM63717 are at least about 10, at least about 11, at least about 12, at least about 13, at least about 14, at least about 15, at least about 16, at least about 17, at least about 18, at least about 19, at least about 20, at least about 21, at least about 22, at least about 23, at least about 24, at least about 25, at least about 30, at least about 35, at least about 40, at least about 45, at least about 50, at least about 60, at least about 70, at least about 80, at least about 90, at least about 100, at least about 150, at least about 200, at least about It may include 250, at least about 300, at least about 400, or at least about 500 consecutive nucleotides. In addition, the present disclosure comprises a nucleotide sequence or any fragment thereof that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to SEQ ID NO. 9.

[0093] Similarly, the fragment of the 5' side (SEQN 11 or SEQN 14) or 3' side (SEQN 12 or SEQN 15) of the soybean event Gm_CSM63717 is SEQN 11 or SEQN 14; or at least about 10, at least about 11, at least about 12, at least about 13, at least about 14, at least about 15, at least about 16, at least about 17, at least about 18, at least about 19, at least about 20, at least about 21, at least about 22, at least about 23, at least about 24, at least about 25, at least about 30, at least about 35, at least about 40, at least about 45, at least about 50, at least about 60, at least about 70, at least about 80, at least about 90, at least about 100, at least about 150, at least about 200, at least about 250, at least about 300, at least about 400, or at least about 500 consecutive of SEQ ID NO. 12 or SEQ ID NO. 15 It may include nucleotides. In addition, the present disclosure includes a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to SEQ ID NO. 11 or 12, or SEQ ID NO. 14 or 15, or any fragment thereof.

[0094] As used herein, the term “about” indicates a value or range of values ​​understood to be equivalent to a specified value, and may be greater or smaller than the specified value or range of values. Each value or range of values ​​preceded by the term “about” is also intended to include an embodiment of the specified absolute value or range of values.

[0095] The term "or" is used herein to mean "and / or" and, unless explicitly stated otherwise, refers only to an alternative or indicates that the alternatives are mutually exclusive. Accordingly, the term "and / or" as used herein in phrases such as "X and / or Y" is intended to include "X and Y," "X or Y," "X (alone)," and "Y (alone)." Likewise, the term "and / or" as used herein in phrases such as "X, Y, and / or Z" is intended to include each of the following embodiments: X (alone); Y (alone); Z (alone); X and Y; X and Z; Y and Z; X, Y, and Z; X, Y, or Z; X or Z; Y or Z; Y or Z.

[0096] When used with the word "comprising" or other open language, the singular form indicates "one or more" unless specifically stated otherwise. The terms "comprising," "having," and "comprising" are open linking verbs. Any form or tense of one or more of these verbs, such as "comprising," "comprising," "having," "comprising," and "including," is also open. For example, any method of "comprising," "having," or "comprising" one or more steps is not limited to having only one or more steps but also encompasses other unlisted steps.

[0097] The soybean event Gm_CSM63717 is characterized by transformative insertion into a locus of the soybean genome to create two new splices (or linkage or connection points). One end of the transformative insert is connected to the lateral soybean genomic DNA by a phosphodiester bond, and the DNA sequence across the linkage is referred to herein as the “sponge.” In other words, the splice is a connection point or covalent link between one end of the transformative insert and the lateral genomic DNA as a single adjacent molecule, formed by inserting a heterologous nucleic acid molecule into the soybean genomic DNA. One splice is found at the 5’ end of the transformative insert and the other splice is found at the 3’ end of the transformative insert, and are referred to herein as the 5’ and 3’ splices, respectively. The “sponge sequence” refers to a DNA sequence of any length of continuous nucleotides across the 5’ or 3’ splice of the transformative event in the plant genome. If the "junction sequence" is specific to the junction between the transformation event and the lateral genome sequence, the junction sequence will generally contain a sufficient number of consecutive nucleotides at one end of the insert and a sufficient number of consecutive nucleotides of the lateral genome sequence.According to some embodiments, although it is understood that the “junction sequence” may be a continuous sequence of nucleotides of any length across the junction of a transformation event in a plant genome, (i) at least 5 (5) continuous nucleotides, at least 10 (10) continuous nucleotides, at least 15 (15) continuous nucleotides, at least 20 (20) continuous nucleotides, at least 25 (25) continuous nucleotides, at least 30 (30) continuous nucleotides, at least 35 (35) continuous nucleotides, at least 40 (40) continuous nucleotides, at least 45 (45) continuous nucleotides, or at least 50 (50) continuous nucleotides at one end of the insert and (ii) at least 5 (5) continuous nucleotides, at least 10 (10) continuous nucleotides, at least 15 (15) continuous nucleotides, at least 20 (20) continuous nucleotides of the side genomic DNA sequence It may include nucleotides, at least 25 (25) consecutive nucleotides, at least 30 (30) consecutive nucleotides, at least 35 (35) consecutive nucleotides, at least 40 (40) consecutive nucleotides, at least 45 (45) consecutive nucleotides, or at least 50 (50) consecutive nucleotides. The junction sequences of soybean event Gm_CSM63717 are not known to appear or exist naturally in the soybean genome or other transgenic soybean events, and these are specific to event Gm_CSM63717 and evident thereto, and various junction sequences of soybean event Gm_CSM63717 can be determined by a person skilled in the art using SEQ ID NO. 10. In SEQ ID NO. 10, the 5' junction is at nucleotides 1,000-1,001 and the 3' junction is at nucleotides 4,201-4,202. Exemplary junction sequences of the soybean event Gm_CSM63717 are provided as sequence numbers 1-8.FIG. 1 illustrates the physical arrangement and location of an exemplary junction sequence arranged from 5' to 3' (left to right) for SEQ ID NO. 10. The DNA sequence for the transforming insert of soybean event Gm_CSM63717 is provided as SEQ ID NO. 9. The DNA sequences of the transforming insert and the soybean genomic DNA on both sides of the transforming insert are provided as SEQ ID NO. 10. The 5' junction sequence is provided as SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7. The 3' junction sequence is provided as SEQ ID NO. 2, SEQ ID NO. 4, SEQ ID NO. 6, and SEQ ID NO. 8. The junction sequence of soybean event Gm_CSM63717 may exist as part of the genome of a plant, seed, plant part, offspring, or plant cell containing soybean event Gm_CSM63717, which is a DNA molecule containing all or part of event Gm_CSM63717. The identification of any one or more junction sequences in a sample of a DNA molecule or plant, plant part, seed, offspring, cell, or product indicates that the DNA molecule or plant, plant part, seed, offspring, cell, or product contains or includes event Gm_CSM63717, was obtained from a soybean plant, plant part, seed, offspring, cell, or product containing or including event Gm_CSM63717, or is a diagnosis of the presence of soybean event Gm_CSM63717.

[0098] The junction sequences described herein are a diagnosis of the presence of all or part of the soybean event Gm_CSM63717. Accordingly, directly or indirectly identifying or detecting one or more of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, and SEQ ID NO. 10 in a sample or DNA molecule derived from a soybean plant, plant part, seed, offspring, cell, or commercial product is a diagnosis that the soybean plant, plant part, seed, offspring, cell, or commercial product has or contains all or part of the soybean event Gm_CSM63717. Directly or indirectly identifying or detecting a 5' junction sequence and / or a 3' junction sequence (each provided or described herein) in a sample or DNA molecule derived from a soybean plant, plant part, seed, offspring, cell, or commercial product is a diagnosis that the soybean plant, plant part, seed, offspring, cell, or commercial product has or contains the soybean event Gm_CSM63717. Accordingly, the present disclosure provides a DNA molecule comprising at least one of the nucleotide sequences provided as SEQ NO. 1, SEQ NO. 2, SEQ NO. 3, SEQ NO. 4, SEQ NO. 5, SEQ NO. 6, SEQ NO. 7, SEQ NO. 8, SEQ NO. 9, and SEQ NO. 10. Any segment of DNA derived from the transgenic soybean event Gm_CSM63717 sufficient to include at least one of the sequences provided in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10 is within the scope of the present disclosure. Furthermore, any DNA or polynucleotide molecule or sequence comprising a sequence complementary to any of the sequences described herein is also within the scope of the present disclosure.

[0099] Polynucleotide molecules are provided that can be used as primers or probes to detect the presence of DNA containing all or part of event Gm_CSM63717 in samples derived from soybean plants, plant parts, seeds, offspring, cells, or commercial products, and these may be single-stranded or double-stranded. Such primers or probes are specific to target polynucleotide sequences and are thus useful for identifying soybean event Gm_CSM63717 polynucleotides by the described method. The primers or probes may hybridize to the target polynucleotide sequence to specifically detect or amplify polynucleotide molecules containing the target polynucleotide sequence or covalently linked and associated with it. Primers and / or probes may be selected to identify and distinguish the detection of specific transformation events as well as the presence of transformation genes in the plant genome. The target polynucleotide sequence may contain all or part of soybean event Gm_CSM63717, junction sequences, and / or side genomic DNA. Probes and primers according to the present disclosure may have (i) complete or 100% sequence complementarity to the target polynucleotide sequence (i.e., 100% complementary) or (ii) incomplete sequence complementarity to the target polynucleotide sequence, such as at least 60% complementary, at least 65% complementary, at least 70% complementary, at least 75% complementary, at least 80% complementary, at least 85% complementary, at least 90% complementary, at least 95% complementary, or at least 99% complementary to the target polynucleotide sequence, provided that the probe or primer has sufficient complementarity to the target polynucleotide sequence to hybridize to the target polynucleotide sequence under strict hybridization conditions required for use of the probe or primer in a related amplification or detection assay, reaction or method.As is understood in the art, longer primers or probes may result in lower percentages of complementarity, depending on the rigor and application. An exemplary polynucleotide molecule is provided that can be used as a primer or probe to detect the presence of soybean event Gm_CSM63717 in a sample. Detecting the presence of soybean event Gm_CSM63717 can be performed using methods known in the art, such as thermal or isothermal amplification of nucleic acids or nucleic acid hybridization techniques (e.g., Northern assay and Southern assay).

[0100] A "probe" is a nucleic acid molecule that is complementary to a target nucleic acid strand and is useful in hybridization detection methods. The probe comprises deoxyribonucleic acid or ribonucleic acid, as well as polyamides and other probe materials that specifically bind to a target DNA sequence, and the detection of such binding may be useful for detecting the presence or absence of the target DNA sequence. The probe may be attached to a conventionally detectable label or reporter molecule, such as a radioisotope, ligand, chemiluminescent agent, or enzyme. Such probes are complementary to the target nucleic acid strand, and in the case of this disclosure, are complementary to the DNA strand of event Gm_CSM63717, whether it is a plant containing event Gm_CSM63717 or a sample containing event Gm_CSM63717 DNA.

[0101] A DNA molecule is provided comprising a polynucleotide segment of sufficient length to function as a DNA probe specific to detecting at least one of the following in a sample: (i) a 5' junction sequence between the lateral soybean genomic DNA and the transform insert of the soybean event Gm_CSM63717; (ii) a 3' junction sequence between the transform insert of the soybean event Gm_CSM63717 and the lateral soybean genomic DNA; (iii) SEQ ID NO. 9; and (iv) a fragment of SEQ ID NO. 9 comprising an adjacent nucleotide of SEQ ID NO. 9 of sufficient length to identify the sequence as a fragment of the transform insert of Gm_CSM63717. An exemplary DNA sequence useful as a probe for detecting the soybean event Gm_CSM63717 is provided as SEQ ID NO. 178. Other DNA sequences useful as probes for detecting the soybean event Gm_CSM63717 are SEQ ID NO. 1; SEQ ID NO. 2; SEQ ID NO. 3; SEQ ID NO. 4; SEQ ID NO. 5; It includes SEQ ID NO. 6; SEQ ID NO. 7; SEQ ID NO. 8; SEQ ID NO. 9; SEQ ID NO. 10; and any of the above complements.

[0102] "Primer" is In a test tube It is a DNA molecule or oligonucleotide designed for use in a specific annealing or hybridization method involving an amplification reaction. A primer pair may be used with template DNA (e.g., a sample of soybean event Gm_CSM63717 genomic DNA) in a thermal amplification reaction (e.g., polymerase chain reaction (PCR)) or any other suitable amplification method known in the art to produce an amplification product or amplicon, wherein the amplicon produced by such reaction will have a DNA sequence corresponding to the sequence of the template DNA located between the two sites where the primer hybridizes to the template DNA.

[0103] DNA amplification reactions, methods, and techniques are known to those skilled in the art. DNA amplification can be achieved by any of the various nucleic acid amplification methods known in the art, including thermal and isothermal amplification methods such as polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). Amplification methods are known in the art and inter alia , U.S. Patent Nos. 4,683,195 and 4,683,202 and PCR Protocols: A Guide to Methods and Applications , ed. Innis etc. It is described in , Academic Press, San Diego, 1990. The PCR amplification method was developed to amplify genomic DNA up to 22 kb (kilobases) and bacteriophage DNA up to 42 kb (Cheng etc. (1994). In addition to these methods, other methods known in the art for DNA amplification may be used in the practice of this disclosure. Examples of DNA amplification methods include PCR, recombinant enzyme polymerase amplification (RPA) ( for example See U.S. Patent No. 7,485,428), strand displacement amplification (SDA)( for example , see U.S. Patent Nos. 5,455,166 and 5,470,723), Transcription-mediated amplification (TMA)( for example , Guatelli etc. (See , 1990), Rolling Circle Amplification (RCA) ( for example , Fire and Xu, 1995; Liu, etc. , 1996; Lizardi, etc. , 1998; see U.S. Patent Nos. 5,714,320 and 6,235,502), helicase-dependent amplification (HDA)( for example Vincent etc. , 2004; see U.S. Patent No. 7,282,328), Multi-Displacement Amplification (MDA)( for example Dean etc. (See , 2002) and loop-mediated isothermal amplifier (LAMP)( for example Notomi etc.Includes (see , 2000). The sequence of a heterologous DNA insert of soybean event Gm_CSM63717 and / or a side genomic DNA sequence can be verified or tested by PCR amplicon or standard DNA sequencing of the cloned DNA fragment thereof after amplifying such DNA molecules in soybean seeds containing event Gm_CSM63717 DNA or in soybean plants grown from soybean seeds containing event Gm_CSM63717 DNA using primers derived from the sequence provided herein.

[0104] As used herein, “amplification product,” “amplified DNA,” or “amplicon” refers to a nucleic acid or DNA molecule or segment produced by a nucleic acid amplification reaction or method as further described herein, which is indicated as a target nucleic acid or DNA molecule that is part of a template nucleic acid molecule. Amplification or amplifying refers to making multiple copies of a target DNA molecule or segment from template DNA. For example, to determine whether a soybean plant, plant part, seed, offspring, or plant cell contains soybean event Gm_CSM63717 due to self-fertilization or crossbreeding of a parent containing soybean event Gm_CSM63717, DNA may be extracted from a soybean plant tissue sample and applied to an amplification reaction or method using a primer pair specific to a target sequence that is part of or uniquely associated with soybean event Gm_CSM63717, such as a first primer derived from a genomic DNA sequence in a region on the side of the heterologous inserted DNA of soybean event Gm_CSM63717 extended by polymerase from 5' to 3' in the direction of the inserted DNA, and a second primer derived from a heterologous inserted DNA molecule extended by polymerase from 5' to 3' in the direction of the side of the genomic DNA from which the first primer was derived. The amplicon may be within a length range that depends on the length of the intervening polynucleotide or DNA sequence between the two primer target sequences in the template DNA molecule. Alternatively, the primer pair may be derived from genomic sequences on both sides of the inserted heterogeneous DNA, so the entire inserted polynucleotide sequence that amplifies a DNA molecule containing the inserted DNA sequence (SEQ No. 9) identified herein in the soybean event Gm_CSM63717 genome ( for example , the 5' end of sequence number 10 ( in other words Forward primer targeted at the genomic region on the upstream of SEQ ID NO. 9 and the 3' end of SEQ ID NO. 10 ( in other wordsAn amplicon containing a reverse primer targeted to a genomic region (downstream of SEQ ID NO. 9) can be produced. The use of the term "amplicon" specifically excludes primer dimers that may be formed in a DNA amplification reaction.

[0105] A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule is provided herein, wherein the first and second DNA molecules are different from each other and comprise a fragment of SEQ ID NO. 10 or its complement, and for producing an amplicon diagnosis for the soybean event Gm_CSM63717 in a sample, for example, the first and second DNA molecules may comprise SEQ ID NO. 176 and SEQ ID NO. 177. The amplicon described herein may comprise a DNA sequence comprising one or more of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, or a fragment of any of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, or SEQ ID NO. 10, wherein the fragment is at least 10 nucleotide long and comprises 1,000-1,001 or 4,201-4,202 nucleotides of SEQ ID NO. 10. According to the present embodiment, the sequence of the amplicon comprises at least one junction sequence or two junction sequences, e.g., a 5' junction sequence and / or a 3' junction sequence for the soybean event Gm_CSM63717. The amplification and detection of such amplicons is an indicator or diagnosis of the soybean event Gm_CSM63717.

[0106] For the purpose of implementation, primers must be designed to produce amplicons within a limited size range, e.g., 100 to 1,000 bases. Smaller (shorter polynucleotide length) amplicons are generally produced more reliably in thermal amplification reactions, allow for shorter cycle times, can be easily separated and visualized on an agarose gel, or can be adopted for use in endpoint TaqMan®-like assays. Smaller amplicons can be produced and detected by methods known in the field of DNA amplicon detection. Furthermore, amplicons produced using the primer pairs can be cloned into a vector, propagated, isolated, and sequenced, or can be sequenced directly by methods well established in the art. Any pair of forward and reverse primers that may be identical to or complementary to parts of SEQ ID NO. 10, such as SEQ ID NO. 176 and SEQ ID NO. 177, which are useful in a DNA amplification method for producing an amplicon diagnosis for soybean event Gm_CSM63717 or its offspring, is an aspect of the present disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 adjacent nucleotides of SEQ ID NO. 10, or its complement which is useful in a DNA amplification method for producing an amplicon diagnosis for soybean event Gm_CSM63717 or its offspring, is an aspect of the present disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 adjacent nucleotides of SEQ ID NO. 11 or SEQ ID NO. 12, or its complement which is useful in a DNA amplification method for producing an amplicon diagnosis for a plant comprising soybean event Gm_CSM63717 or its offspring, is an aspect of the present disclosure.Any single isolated DNA polynucleotide primer molecule comprising at least 15 adjacent nucleotides of SEQ ID NO. 9, or its complement useful for a DNA amplification method for producing an amplicon diagnosis for soybean event Gm_CSM63717 or its offspring, is an aspect of the present disclosure.

[0107] Primers are typically designed to specifically hybridize to a complementary target DNA strand to form a hybrid between the primer and the target DNA strand. Hybridization or binding of the primer to the complementary target DNA strand is a recognition site by a polymerase that initiates the expansion of the primer using the target DNA strand as a template ( in other words It is the polymerization of additional nucleotides and elongated nucleotide molecules. A primer pair typically refers to the use of two primers that combine opposite strands of a double-stranded nucleotide segment for the purpose of amplifying a polynucleotide segment between targeted sites for binding by individual members of the primer pair in a heat amplification reaction or other conventional nucleic acid amplification reaction. Primer pairs are typically designed to hybridize to different nearby target sites of the template DNA molecule on opposite strands of the template DNA molecule, allowing the intervening region or sequence between the two primers to be specifically amplified for use or detection through multiple amplification rounds.

[0108] To detect the presence or absence of soybean event Gm_CSM63717, the target site and / or intervening region or sequence of the template DNA molecule may include at least one junction sequence and / or at least a portion of the insertion of soybean event Gm_CSM63717. To detect the absence of soybean event Gm_CSM63717, the target site and / or intervening region or sequence of the template DNA molecule may include soybean genomic DNA that does not include any portion of the junction sequence or the insertion of soybean event Gm_CSM63717. Thus, the presence or absence of an amplicon with a primer pair can diagnose the presence or absence of soybean event Gm_CSM63717 in the DNA molecule or sample, respectively, or vice versa. This may also be possible with more than one primer pair. For example, the first primer pair can produce a first amplicon when the soybean event Gm_CSM63717 is present, and the second primer pair can produce a second amplicon when the soybean event Gm_CSM63717 is absent or not present. Alternatively, the size of the amplicon produced in the amplification reaction may also be a diagnosis of the presence or absence of the soybean event Gm_CSM63717 in the DNA molecule or sample, and - for example, the primer pair can produce a first amplicon of the first size when the soybean event Gm_CSM63717 is present or a second amplicon of the second size when the soybean event Gm_CSM63717 is absent or not present; the first primer pair can produce a first amplicon of the first size when the soybean event Gm_CSM63717 is present, and the second primer pair can produce a second amplicon of the second size when the soybean event Gm_CSM63717 is absent or not present. According to some of these embodiments, at least two primer pairs may be used, wherein at least one of the primer pairs is used as an internal control and is not associated with the soybean event Gm_CSM63717.

[0109] According to the present embodiment, a primer pair for detecting the presence or absence of all or part of the soybean event Gm_CSM63717 in a DNA molecule or sample comprises a first primer and a second primer, wherein the first primer is complementary to the 5' side genomic DNA sequence and the second primer is complementary to the sequence within the transform insert; wherein the first primer is complementary to the 5' side genomic DNA sequence and the second primer is complementary to the 3' side genomic DNA sequence; wherein the first primer is complementary to the sequence within the transform insert and the second primer is complementary to the 3' side genomic DNA sequence. In this paragraph, each reference to a primer complementary to a sequence within the 5' side genomic DNA sequence, the 3' side genomic DNA sequence, or the sequence within the transformation insert of soybean event Gm_CSM63717 is also intended to potentially include a primer complementary to the reverse complement or opposite strand of the sequence within the 5' side genomic DNA sequence, the 3' side genomic DNA sequence, or the sequence within the transformation insert of soybean event Gm_CSM63717, respectively.

[0110] Exemplary DNA molecules useful as primers for detecting soybean event Gm_CSM63717 are provided as SEQ ID NO. 176 and SEQ ID NO. 177. The primer pair SEQ ID NO. 176 and SEQ ID NO. 177 may be useful as a first primer (complementary to the sequence in the transform insert) and a second primer (corresponding to the 5' side genomic DNA sequence), wherein each primer has a sequence of SEQ ID NO. 10 of sufficient length or a sequence complementary to SEQ ID NO. 10, so that when used with template DNA derived from soybean event Gm_CSM63717 in an amplification reaction, it hybridizes to the opposite strand of the template DNA and functions as a DNA primer that produces an amplicon diagnosis for soybean event Gm_CSM63717 DNA in the sample. Primer pair SEQ ID NO. 208 (complementary to the 3' lateral genomic DNA sequence) and SEQ ID NO. 209 (corresponding to the 5' lateral genomic DNA sequence) are useful as first and second primers, respectively, wherein each primer has a continuous nucleotide of sufficient length for a locus within the soybean genome and, when used with template DNA in a heat amplification reaction, functions as a DNA primer that produces an amplicon indicator or diagnosis for wild-type DNA regarding the conjugation of Gm_CSM63717 event DNA in the sample. The amplicon diagnosis for event Gm_CSM63717 includes a sequence not naturally found in the soybean genome.

[0111] The primers are Kompetitive Allele-Specific PCR (KASP) TMIt may additionally include oligo-tail sequences similar to those used in the method. Each allele-specific primer possesses a unique tail sequence corresponding to a universal FRET (Fluorescence Resonance Energy Transfer) cassette; one is labeled with a FAM dye and the other with a HEX dye. During thermal cycling, the relevant allele-specific primer binds to the template and extends, so that the tail sequence is attached to the newly synthesized strand. Then, the complement of the allele-specific tail sequence is generated during subsequent PCR rounds, allowing the FRET cassette to bind to DNA. The FRET cassette is no longer quenched and emits fluorescence.

[0112] Methods for designing and using primers and probes are well known in the art. DNA molecules containing fragments of SEQ ID NOs. 1-10 are useful as primers and probes for detecting the soybean event Gm_CSM63717 and can be easily designed by those skilled in the art using the sequences provided herein. These probes and primers are selected to have sufficient length and sequence complementarity to the target sequence to specifically hybridize to the target sequence under strict hybridization conditions. While probes and primers may have complete sequence complementarity or identity with the target sequence, probes and primers that differ from the target sequence in terms of identity or complementarity but maintain the ability to form a stable double-stranded structure and hybridize to the target sequence under specific hybridization or reaction conditions can be designed by conventional methods.

[0113] The presence of target DNA from a transgenic plant, such as the soybean event Gm_CSM63717, can be identified or detected in a sample using any conventional nucleic acid hybridization or amplification method. Thus, a polynucleotide molecule or DNA molecule, also referred to as a "polynucleotide segment or fragment of sufficient length" or "adjacent or consecutive nucleotides of sufficient length," can specifically hybridize to a target DNA sequence under specific hybridization or reaction conditions. As used herein, the term "sufficient length" refers to any length sufficient to be useful in a selected detection method. Probes and primers are generally at least about 8 nucleotides, at least about 10 nucleotides, at least about 12 nucleotides, at least about 14 nucleotides, at least about 16 nucleotides, at least about 18 nucleotides, at least about 20 nucleotides, at least about 22 nucleotides, at least about 24 nucleotides, at least about 26 nucleotides, at least about 28 nucleotides, or at least about 30 nucleotides or longer. These probes and primers specifically hybridize to the target DNA sequence under strict hybridization conditions.

[0114] As used herein, two nucleic acid molecules can specifically hybridize with each other if the two molecules can form an antiparallel, double-stranded nucleic acid structure. A nucleic acid molecule is the "complement" of another nucleic acid molecule if it exhibits complete complementarity. As used herein, two nucleic acid molecules are "completely complementary" if they exhibit "complete complementarity" and are complementary to all nucleotides of the second nucleic acid molecule when all nucleotides of the first nucleic acid molecule are aligned. Two molecules are "minimally complementary" if they can hybridize with each other with sufficient stability to allow them to maintain annealing with each other under at least conventional "low severity" conditions. Similarly, molecules are "complementary" if they can hybridize with each other with sufficient stability to allow them to maintain annealing with each other under conventional "high severity" conditions. Conventional severity conditions are Haymes etc. , In: Nucleic Acid Hybridization, A Practical Approach , IRL Press, Washington, DC (1985), and MR Green and J Sambrook, Molecular cloning: a laboratory manual , 4 th This is described in Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2012). Therefore, deviation from complete complementarity is acceptable as long as such deviation does not completely exclude the molecule's ability to form a double-stranded structure. For a nucleic acid molecule to serve as a primer or probe, it only needs to be sufficiently complementary in sequence to form a stable double-stranded structure under specific solvent and salt concentrations and other conditions used.

[0115] As used herein, a sequence substantially homologous or complementary with respect to a reference nucleic acid sequence is a nucleic acid sequence that specifically hybridizes to the reference nucleic acid sequence or its complement being compared under high-strictness conditions. As used herein, "strict hybridization conditions" refers to conditions in which a polynucleotide will typically hybridize to a target sequence in a complex mixture of nucleic acids, but not to a sequence that is essentially different. When referring to a polynucleotide probe, "strict conditions" or "strict hybridization conditions" refer to a degree in which the probe is detectably greater than other sequences ( for example It refers to conditions for hybridization to a target sequence at least 2-fold relative to the background. Strict conditions are sequence-dependent and will vary under different circumstances. Longer sequences hybridize specifically at high temperatures. Generally, strict conditions are defined as the thermal melting point (T) for a specific sequence at a defined ionic strength and pH. m About 5-10 times more than ) It is selected to be lower. T m is the temperature at which 50% of probes complementary to the target hybridize to the target sequence at equilibrium (since the target sequence is excessively present, T m (At equilibrium, it accounts for 50% of the probe). Strict conditions are a salt concentration of less than about 1.0 M sodium ions at pH 7.0 to 8.3, typically about 0.01 to 1.0 M sodium ion concentration (or other salts), and a probe with a short temperature ( for example In the case of , 10 to 50 nucleotides), at least about 30 This long probe ( for example In the case of more than 50 nucleotides, at least about 60 It is likely. Strict conditions can also be achieved by adding a destabilizing agent such as formamide. By controlling the strictness of hybridization and / or washing conditions, a target sequence that is 100% complementary to the probe can be identified (homology probing). Alternatively, strictness conditions can be adjusted to allow some discrepancies in the sequence, thereby enabling the detection of a lower degree of identity (heterogeneous probing).

[0116] Appropriate rigor conditions that promote DNA hybridization, e.g., about 45 From 6x sodium chloride / sodium citrate (SSC), followed by 50 Cleaning from 2xSSC is known to those skilled in the art or Current Protocols in Molecular Biology This can be found in John Wiley & Sons, NY (1989), 6.3.1–6.3.6. For example, in the washing step, the salt concentration is 50 At low strictness of about 2.0 x SSC, 50 It can be selected from high strictness of approximately 0.2 x SSC. In addition, during the washing step, the temperature is room temperature, approximately 22 At low strictness conditions, about 65 Conditions of high severity can be increased from [indicated] to [indicated]. Both temperature and salt may vary, or either temperature or salt concentration may be kept constant while the other variable changes. In relation to amplifying a target polynucleotide using a specific amplification primer pair (e.g., by PCR), "strict conditions" or "strict hybridization conditions" are conditions that cause the primer pair to hybridize to the target polynucleotide to which the primer having the corresponding sequence (or its complement) will bind, and to produce an identifiable amplification product (amplicon) having the soybean Gm_CSM63717 event-specific region in the DNA thermal amplification reaction. The term "specific" to the target sequence indicates that the probe or primer hybridizes only to the target sequence in a sample containing the target sequence under strict hybridization conditions.

[0117] A polynucleotide molecule or DNA molecule of the present disclosure, such as a primer or probe, is formed under strict hybridization conditions, or under moderately strict hybridization conditions where the sequence of the polynucleotide molecule is not identical to at least one of the nucleic acid molecules, at least one of the nucleic acid molecule sequences selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, Or it will specifically hybridize to a polynucleotide having at least 99.9% identical nucleotide sequence, or to the complete complement or fragment of any of the above. Hybridization of a nucleic acid molecule, such as a primer or probe, to a target DNA molecule can be detected by any number of methods known to those skilled in the art, which may include, but are not limited to, fluorescent tags, radioactive tags, antibody-based tags, and chemiluminescent tags.

[0118] An exemplary DNA molecule or polynucleotide useful as a probe for detecting the soybean event Gm_CSM63717 is provided as SEQ ID NO. 178. In some embodiments, the DNA molecule functioning as a probe comprises a nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, a complement of any of the foregoing, or a fragment of any of the foregoing. In other embodiments, the DNA molecule comprises a polynucleotide segment of sufficient length to function as a DNA probe specific to at least one of the following: a) a 5' junction sequence between the lateral soybean genomic DNA and the transforming insert of the soybean event Gm_CSM63717; b) a 3' junction sequence between the transforming insert of the soybean event Gm_CSM63717 and the lateral soybean genomic DNA; c) SEQ ID NO. 9; or d) a fragment of SEQ ID NO. 9 comprising adjacent nucleotides of SEQ ID NO. 9 of sufficient length to identify the sequence as a fragment of a transform insert of Gm_CSM63717 in a DNA sample.

[0119] Diagnostic amplicons produced by the methods described herein may be detected by a plurality of techniques known in the art, such as sequencing, restriction mapping, Southern analysis, or any other suitable polynucleotide or DNA hybridization, blotting, polymerization and / or amplification-based approaches or techniques. One method is Genetic Bit Analysis (Nikiforov etc. , 1994) and here adjacent lateral genomic DNA sequences and inserted DNA sequences - in other wordsDNA oligonucleotides are designed to overlap both the insertion sequence and the junction sequence. The oligonucleotides are immobilized in wells of a microtiter plate. After PCR of the region of interest (e.g., using one primer for the insertion sequence and one primer for the adjacent lateral genomic sequence), the single-stranded PCR product is hybridized to the immobilized oligonucleotides to serve as a template for a single-base expansion reaction using DNA polymerase and labeled dideoxynucleotide triphosphate (ddNTP) specific to the expected next base. Readouts can be fluorescence- or ELISA-based. The signal indicates the presence of the transformed gene / genome junction sequence resulting from successful amplification, hybridization, and single-base expansion.

[0120] Another method is the pyrosequencing technique described by Winge (2000). In this method, an oligonucleotide is designed to overlap adjacent genomic DNA and the insertion DNA junction. The oligonucleotide is hybridized to a single-stranded PCR product of the region of interest (one primer for the insertion sequence and one primer for the adjacent genomic sequence) and incubated in the presence of DNA polymerase, ATP, sulfurylase, luciferase, apyrase, adenosine 5' phosphosulfate, and luciferin. The addition of DNTPs individually and incorporation generates a light signal to be measured. The light signal indicates the presence of the transformed gene / genomic sequence resulting from successful amplification, hybridization, and single or multiple base extension.

[0121] Chen etc.Fluorescence polarization as described by (1999) is a method that can be used to detect the amplicon of the present invention. Using this method, an oligonucleotide is designed to overlap the genomic side and the inserted DNA junction. The oligonucleotide is hybridized to a single-stranded PCR product of the region of interest (one primer for the inserted DNA and one primer for the lateral genomic DNA sequence) and incubated in the presence of DNA polymerase and fluorescently labeled ddNTPs. Single base expansion results in the incorporation of ddNTPs. Incorporation can be measured as a change in polarization using a fluorescence meter. A change in polarization indicates the presence of the transformed gene / genomic sequence resulting from successful amplification, hybridization, and single base expansion.

[0122] Real-time polymerase chain reaction (PCR) occurs when ( in other words It has the ability to monitor the progress of PCR in real-time. Data is collected throughout the PCR process, rather than at the end of the PCR. In real-time PCR, the reaction is characterized by the point in time during the cycle when target amplification is first detected, rather than the amount of target accumulated after a fixed number of cycles. In real-time PCR assays, a positive reaction is detected by the accumulation of the fluorescence signal. The higher the starting copy number of the nucleic acid target, the faster a significant increase in fluorescence is observed. The cycle threshold (Ct value) is the amount required for the fluorescence signal to cross the threshold ( in other words , exceeding background level) is defined by the number of cycles. The Ct level is inversely proportional to the amount of target nucleic acid in the sample ( in other words , the lower the Ct value, the greater the amount of target nucleic acid in the sample).

[0123] Taqman® (PE Applied Biosystems, Foster City, California) is a method for detecting and quantifying the presence of DNA sequences using real-time PCR and is fully described by the instructions provided by the manufacturer. Briefly, the FRET oligonucleotide probe is designed so that the genomic lateral and insertion DNA junctions overlap. The FRET probe and PCR primers (one primer for the insertion DNA sequence and one primer for the lateral genomic sequence) are cycled in the presence of heat-stable polymerase and dNTPs. Hybridization of the FRET probe cleaves the fluorescent moiety from the quenching moiety of the FRET probe and releases the fluorescence signal. The fluorescent signal indicates the presence of the transformed gene / genomic sequence resulting from successful amplification and hybridization.

[0124] Molecular beacons are Tyangi etc. It was described for use in sequence detection as described in (1996). Briefly, a FRET oligonucleotide probe is designed to overlap the lateral genome and the insertion DNA junction. The intrinsic structure of the FRET probe contains a secondary structure that maintains the fluorescent and quenching moiety in close proximity. The FRET probe and PCR primers (one primer for the insertion DNA sequence and one primer for the lateral genome sequence) are cycled in the presence of a heat-stable polymerase and dNTPs. After successful PCR amplification, hybridization of the FRET probe to the target sequence removes the probe's secondary structure and spatially separates the fluorescent and quenching moiety. A fluorescent signal is generated, indicating the presence of the lateral / transformation gene insertion sequence resulting from successful amplification and hybridization.

[0125] Other detection methods known in the art may be used. For example, microfluidic ( for example(See U.S. Patent Publication No. 2006 / 068398; U.S. Patent No. 6,544,734) provides a method and apparatus that can be used to isolate and amplify DNA samples or molecules. Specific DNA molecules can be detected and measured using optical dyes ( for example , see WO / 05017181). Then, a nanotube device including an electronic sensor for detecting nanobeads that bind to a DNA molecule or a specific DNA molecule ( for example , refer to WO / 06024023) may be detected. Wang etc. (2021), Tyler etc. (2018), or Pearson etc. Nanopore sequencing technology, such as that described in (2019), can also be used for event detection.

[0126] Accordingly, the DNA molecule and corresponding nucleotide sequence provided herein are particularly useful for identifying soybean event Gm_CSM63717, detecting the presence of DNA derived from transgenic soybean event Gm_CSM63717 in a sample, and monitoring the sample for the presence or absence of soybean event Gm_CSM63717 or plant parts derived from soybean plants containing event Gm_CSM63717.

[0127] Proteins are provided that can be used to produce antibodies for detecting the presence of soybean event Gm_CSM63717 in a sample. These antibodies are specific to the PPO protein encoded by soybean event Gm_CSM63717. Methods for producing polyclonal or monoclonal antibodies are well known to those skilled in the art and can be used to produce antibodies specific to the PPO protein encoded by soybean event Gm_CSM63717. For example, Lermontova et al. (1997) describe antibodies against the PPO protein. A DNA sequence encoding the PPO protein is provided at SEQ ID NO. 10, and the start and end positions of the coding sequence are indicated in Table 1. The DNA sequence encoding the protein and the protein encoded by the sequence are useful for producing antibodies for detecting the presence of soybean event Gm_CSM63717 by the method described herein. Detection of the presence of soybean event Gm_CSM63717 may be performed using any protein detection technique known in the art, such as Western blot analysis, immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), antibody attachment to detectable labels or reporter molecules (e.g., radioisotopes, ligands, chemiluminescent agents, or enzymes), or enzymatic action on reporter molecules. One method involves contacting a sample with an antibody that binds to the PPO protein encoded by soybean event Gm_CSM63717 and then detecting the presence or absence of antibody binding. The binding of such antibodies is a diagnosis of the presence of one or more proteins encoded by soybean event Gm_CSM63717.

[0128] An alternative to antibodies for protein detection is an aptamer-based detection method for detecting proteins or molecules of interest in a sample. As used herein, the terms “aptamer(s)” or “aptamer sequence(s)” refer to short synthetic single-stranded oligonucleotide molecules having high affinity and specificity that bind to target molecules, such as proteins, polypeptides, lipids, glycoproteins, glycolipids, glycopeptides, sugars, or polysaccharides, by forming distinct tertiary structures (Ellington and Szostak, 1990; Robertson and Joyce, 1990; Tuerk and Gold, 1990; Wang et al., 2019). The single-stranded nucleic acid may be ssDNA, RNA, or a derivative thereof. An aptamer comprises a three-dimensional structure maintained in a specific form(s) that provides intermolecular contacts that specifically bind to a given target. Aptamers are nucleic acid-based molecules, but their binding to target molecules does not depend entirely on linear base sequences, but rather on specific secondary, tertiary, or quaternary structures. The term aptamer also encompasses next-generation aptamers, such as X aptamers, which cannot typically be amplified by PCR but can be adopted by adding link primers. These aptamers can bind specifically to proteins of interest but can also be easily amplified and sequenced in downstream processes. The term aptamer also encompasses aptamers containing modified bases. Aptamers can include traditional aptamers ranging from 15 to 120 bases in length, as well as longer aptamers of approximately 200 bases (e.g., Ultramers® by Integrated DNA Technologies, Inc. of Coralville, Iowa). To detect a protein of interest in a sample, an aptamer specific to the protein of interest is obtained and then incubated with the sample. If the protein of interest is present in the sample, a protein-aptamer conjugate is formed.Methods for detecting aptamer / protein complexes are known in the art, such as aptablotting or South-Western blot (Li et al., 2017; Sekhon et al., 2017), aptamer-based Western blot (Wang et al., 2020), and aptamer sandwich assay (Svobodova et al., 2021). Chemical modifications, additional functional groups, and / or linkers may be added to nucleic acid aptamers to provide increased binding affinity for target proteins and to provide convenient means for detecting target molecules. Such tags and / or labels may include fluorescence, luminescence, absorbance, or radioactive-based chemical groups, or may include enzymes or substrates that provide detectable reactions, such as precipitation, either alone or in the presence of other factors.

[0129] A nucleic acid or protein detection kit for detecting the presence of soybean event Gm_CSM63717 is provided. Variations in such kits may also be developed using the compositions and methods disclosed herein and methods well known in the art for proteins and nucleic acids for the identification of soybean event Gm_CSM63717. Protein and nucleic acid detection kits may be applied to methods of breeding plants containing soybean event Gm_CSM63717. Such kits contain primers and / or probes or antibodies or aptamers specific to soybean event Gm_CSM63717. Such DNA primers and / or probes may include one or more fragments of SEQ ID NOs 1-10, or antibodies specific to proteins encoded by soybean event Gm_CSM63717. The kit may also contain instructions for using the primers, probes, or antibodies or aptamers for detecting the presence of soybean event Gm_CSM63717. The kit may optionally also include reagents for performing the detection or diagnostic reactions described herein.

[0130] One example of a detection kit comprises at least one DNA molecule of adjacent nucleotides of SEQ ID NO. 10 of sufficient length to function as a DNA probe useful for detecting the presence or absence of soybean event Gm_CSM63717 in a sample. DNA derived from a transgenic soybean plant containing event Gm_CSM63717 will comprise a DNA molecule having at least one sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, a complement of any of the foregoing, or a fragment of any of the foregoing. An exemplary DNA molecule sufficient for use as a probe is one comprising the sequence provided in SEQ ID NO. 178. Other probes may be readily designed by those skilled in the art. The probe may include a junction sequence spanning the 5' or 3' junction between the soybean genomic DNA and the transform insert of the soybean event Gm_CSM63717.

[0131] Another example of a detection kit comprises at least one pair of primers that specifically hybridize to target DNA and amplify a diagnostic amplicon under appropriate reaction conditions useful for detecting the presence or absence of the soybean event Gm_CSM63717 in a sample. It is the object of the present disclosure to have a kit containing DNA primers homologous to or complementary to any portion of the soybean genome region as presented in SEQ ID NO. 11 or 12 and any portion of the inserted transformed DNA as presented in SEQ ID NO. 9. The kit may provide an agarose gel-based detection method or any number of amplicon detection methods known in the art. Such methods may also include sequencing the amplicon or a fragment thereof. An exemplary DNA molecule sufficient for use as a primer pair comprises the sequences provided as SEQ ID NO. 176 and SEQ ID NO. 1177 and SEQ ID NO. 208 and SEQ ID NO. 209, respectively, wherein the primer pair SEQ ID NO. 176 and SEQ ID NO. 177 will produce an amplicon diagnosis for the presence of event Gm_CSM63717 in a sample; and the primer pair SEQ ID NO. 208 and SEQ ID NO. 209 will produce an amplicon representing wild-type DNA, thus a diagnosis for the absence of event Gm_CSM63717 in a sample. Other primer pairs may be readily designed by those skilled in the art.

[0132] Another example of a detection kit includes an antibody specific to the PPO protein encoded by the soybean event Gm_CSM63717. For example, such a kit may utilize a side-flow strip containing a reagent that is activated when the end of the strip comes into contact with an aqueous solution. An exemplary protein sufficient for use in antibody production is the PPO protein encoded by the sequence provided as SEQ ID NO. 10, or any fragment thereof. The detection of binding of the antibody to the PPO protein encoded by the soybean event Gm_CSM63717 in a sample is a diagnosis of the presence of the soybean event Gm_CSM63717 in the sample.

[0133] The detection kit provided herein is particularly useful for identifying soybean event Gm_CSM63717, selecting plant varieties or hybrids containing soybean event Gm_CSM63717, detecting the presence of DNA derived from transgenic soybean plants containing event Gm_CSM63717 in a sample, and monitoring the sample for the presence and / or absence of soybean plants containing event Gm_CSM63717, or plant parts derived from soybean plants containing event Gm_CSM63717.

[0134] Soybean plants, offspring, seeds, cells, and plant parts containing the soybean event Gm_CSM63717, as well as commercial products produced using these are provided. As used herein, the terms "soybean" or "bean" are Glycine Max Plant species within and Glycine Including wild soybean species such as Glycine Max Genus that can be bred with plants GlycineIt refers to all plant varieties belonging to ). The term “soybean” is intended to include soybean plants, plant parts, plant cells, plant tissues, seeds, offspring plants, and / or soybean product products. These soybean plants, plant parts, plant cells, plant tissues, seeds, offspring plants, and product products are derived from transgenic soybean plants, plant parts, plant cells, plant tissues, seeds, offspring plants, or product products containing or including soybean event Gm_CSM63717. These soybean plants, plant parts, plant cells, plant tissues, seeds, offspring plants, and commercial products comprise a detectable amount of polynucleotide or DNA molecule comprising at least one junction sequence and / or heterologous transformation insert sequence of the soybean event Gm_CSM63717, e.g., a polynucleotide or nucleic acid or DNA molecule having or comprising at least one of the sequences provided as SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10; a polynucleotide comprising at least 16 consecutive nucleotides of SEQ ID NO. 1, at least 16 consecutive nucleotides of SEQ ID NO. 2, at least 33 consecutive nucleotides of SEQ ID NO. 3, at least 32 consecutive nucleotides of SEQ ID NO. 4, at least 53 consecutive nucleotides of SEQ ID NO. 5, or at least 52 consecutive nucleotides of SEQ ID NO. 6; the entire length of SEQ ID NO. 10 or the entire length of SEQ ID NO. 9 and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% polynucleotides containing identical sequences, and the complete complement of any of the foregoing. In some embodiments, a soybean plant, plant part, plant cell, plant tissue, or seed is further defined as a soybean plant containing the soybean event Gm_CSM63717, or a soybean plant part, plant seed, or offspring plant of any generation derived therefrom.

[0135] As used herein, "soy event Gm_CSM63717" or "soy event Gm_CSM63717 locus" refers to a genomic locus of soy event Gm_CSM63717 or a modified soy event Gm_CSM63717 comprising complete or partial aspect, junction, and insertion sequences of soy event Gm_CSM63717 or a modified soy event Gm_CSM63717. A modified soy event Gm_CSM63717 comprises one or more mutations, edits, and / or genetic modifications, such as deletions, insertions, inversions, or translocations at the soy event Gm_CSM63717 locus compared to soy event Gm_CSM63717.

[0136] A modified soybean event Gm_CSM63717 and a method for preparing the modified soybean event Gm_CSM63717 are provided. As further described herein, various mutagenic or targeted genome editing techniques and related tools may be prepared or manipulated to allow genetic modification or mutation of the transforming inserts, junctions, and / or flanking genomic DNA of the soybean event Gm_CSM63717 by, for example, deletion, insertion, translocation, inversion, and / or substitution of nucleic acid sequences, or by insertion or introduction of guide RNA target sites or cognate target sites or CgRRS, and the modified transforming event may still uniquely characterize the presence of one or more sequences of the heterogeneous DNA and / or inserts, junctions, and / or flanking sequences described herein at specific locations within the genome previously occupied by the unmodified soybean event Gm_CSM63717 relative to the flanking portion or sequence of the natural soybean genome. According to the present embodiment, a modified transformation event derived from soybean event Gm_CSM63717 may comprise the insertion sequence of soybean event Gm_CSM63717 described herein and / or all or part of the transformation cassette and / or one or more complete or partial side sequences. As used herein, "modified soybean event Gm_CSM63717" refers to the genomic DNA or sequence of the soybean event Gm_CSM63717 locus comprising one or more mutations, edits, or genetic modifications to the genomic DNA or sequence of soybean event Gm_CSM63717, wherein such mutations, edits, or genetic modifications are introduced or made by mutagenic or targeted genome editing techniques on soybean plants, plant parts, tissues, or cells containing soybean event Gm_CSM63717."Modified soybean event Gm_CSM63717" comprises "additionally modified soybean event Gm_CSM63717" prepared by first inserting a target site or a cognate target site or CgRRS into the soybean event Gm_CSM63717 locus and then further modifying the soybean event Gm_CSM63717 locus as described herein. Methods and techniques for mutagenesis are known in the art, for example, chemical mutagenesis. (in other words, Azide, hydroxylamine, nitrous acid, acridine, nucleotide base analogs, or alkylating agents - for example , treatment with chemical mutagens such as EMS (ethylmethanesulfonate), MNU (N-methyl-N-nitrosourea), etc.), physical mutagenesis (for example, gamma rays, X-rays, UV, ion beams, other forms of radiation, etc.), and insertable mutagens (for example, It includes a transposon or T-DNA insert. The soybean plants, plant parts, plant seeds, plant tissues, and plant cells described herein include soybean plants, parts, seeds, tissues, and cells containing modified soybean event Gm_CSM63717 or additional modified soybean event Gm_CSM63717.

[0137] Soybean plants, plant parts, plant cells, plant tissues, seeds, offspring plants, and commercial products express or contain PPO herbicide resistance genes, e.g., flumioxazine, epirifenacil, lactofen, asifluorofen, pyraflufen, pyraflufen-ethyl, oxadiazone, butapenacil, 피리딘-2-일메틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-시아노-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 시아노메틸 [(3-{2-브로모-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 사이클로프로필메틸 (2-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}페녹시)아세테이트, It is resistant to one or more PPO herbicides including but not limited to methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoxysimil), fomesafene, saflufenacil, sulfentrazone, tiafenacil, trifludimoxazine, and any combination thereof.

[0138] The present disclosure provides soybean plants, offspring, seeds, plant cells, and plant parts such as microspores, pollen, anthers, ovules, ovaries, flowers, pods, embryos, stems, buds, nodes, leaves, roots, and callus tissues derived from transgenic soybean plants containing soybean event Gm_CSM63717. Representative samples of seeds containing soybean event Gm_CSM63717 were deposited pursuant to the Budapest Treaty for the purpose of enabling the present disclosure. The ATCC repository assigned accession number PTA-127604 to the seeds containing soybean event Gm_CSM63717.

[0139] A soybean plant, plant seed, plant part, or plant cell may additionally contain at least one additional transgenic gene for resistance to at least one additional herbicide. For example, the additional transgenic gene may be selected from the group consisting of phosphinothricin N-acetyltransferase (PAT), dicamba monooxygenase (DMO), FT_T, triketone dioxygenase (TDO), 5-enolpyrubilchimate-3-phosphate synthase (EPSPS), and any combination thereof. Streptomyces viridochromogenes An exemplary PAT codon-optimized coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO. 26 and SEQ ID NO. 27, respectively. Stenotrophomonas maltophilia (also known as Pseudomonas maltophilia) An exemplary DMO codon-optimized coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO. 28 and SEQ ID NO. 29, respectively. Sphingobium herbicidoborans An exemplary FT_Tv7 coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO. 30 and SEQ ID NO. 31, respectively. Oriza Sativa An exemplary TDO codon-optimized coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO. 32 and SEQ ID NO. 33, respectively. AgrobacteriumAn exemplary EPSPS codon-optimized coding sequence of a CP4 strain and its corresponding amino acid sequence are provided as SEQ ID NO. 34 and SEQ ID NO. 35, respectively. For example, a soybean plant, plant seed, plant part, or plant cell may further contain soybean event Gm_CSM63714 and / or MON89788. Genetic elements in the transform inserts of soybean event Gm_CSM63714 and soybean event MON89788 are described below.

[0140] Additional transgenic genes are glutamine synthetase inhibitors ( for example , glufosinate), 4-hydroxyphenylpyruvate deoxygenase inhibitor ( for example , mesotrione), synthetic auxin, e.g. benzoate auxin ( for example , dicamba) and phenoxyauxin ( for example , 2,4-D), inhibitors of EPSPS ( for example It can provide resistance to herbicides having different modes of action selected from the group consisting of glyphosate, and any combination thereof.

[0141] A microorganism is provided. The microorganism comprises a polynucleotide molecule having a nucleotide sequence identical to the nucleotide sequence of SEQ ID NO. 9, or the entire length of SEQ ID NO. 9 by at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9%. Examples of such microorganisms are Agrobacterium It is a cell. Another example of such a microorganism is E. coli ( E. coli It is a cell.

[0142] A plant cell comprising a polynucleotide molecule as described herein is provided. For example, a plant cell having a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10 present in the genome, and a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO. 10 or the full length of SEQ ID NO. 9 is provided.

[0143] The plant cells and microorganisms of the present disclosure are useful for many commercial applications, including but not limited to (i) use as research tools for scientific investigation or industrial research; (ii) use in cultures to produce endogenous or recombinant carbohydrate, lipid, nucleic acid, enzyme, or protein products, or small molecules that can be used for subsequent scientific research or industrial products; and (iii) in the case of the plant cells of the present disclosure, use in conjunction with modern plant tissue culture techniques to produce transgenic plants or plant tissue cultures that can be used for agricultural research or production. The production and use of such transgenic plant cells utilize modern microbiological techniques and human intervention to produce artificial, unique plant cells. In this process, recombinant DNA is inserted into the genome of the plant cell to separate it from naturally occurring plant cells and generate unique transgenic plant cells. These transgenic plant cells can then be cultured using modern microbiological techniques to closely resemble bacterial and yeast cells and may exist in an undifferentiated single-cell state. The genetic composition and phenotype of the new plant cells are technical effects created by integrating heterologous DNA into the cell's genome.

[0144] Methods are provided for using plant cells such as transformed plant cells. These include: (i) a method for producing transformed cells by incorporating recombinant DNA into the genome of a cell and then using this cell to induce additional cells possessing the same heterologous DNA; (ii) a method for culturing cells containing recombinant DNA using modern microbiological techniques; (iii) a method for producing and purifying endogenous or recombinant carbohydrates, lipids, nucleic acids, enzymes, or protein products from cultured cells; and (iv) a method for producing transformed plants or transformed plant tissue cultures using modern plant tissue culture techniques with transformed plant cells.

[0145] Plants, offspring, seeds, cells, and plant parts may contain one or more additional desired trait(s). These desired traits may be transformative traits, native traits, or traits produced by other methods such as genome editing, base editing, prime editing, or other conventional mutagenesis methods. The desired trait may be combined with the soybean event Gm_CSM63717, for example, by crossing a soybean plant containing the soybean event Gm_CSM63717 with another soybean plant containing the additional trait(s) or the transformative event. These traits or transformative events include, but are not limited to, increased insect tolerance, increased water use efficiency, increased yield performance, increased drought tolerance, increased disease tolerance, increased seed quality, improved nutrient quality, production of hybrid seeds, and / or increased herbicide tolerance, wherein the trait is measured in relation to a soybean plant lacking these transformative traits. For example, the Gm_CSM63717 event may be stacked with other events or combinations of events known in the art, including but not limited to the following, by breeding, by retransformation, or by site-specific integration / gene transfer:

[0146] · A2704-12 (also known as Liberty Link® soybeans for EE-GM1, LL27, and glufosinate herbicide resistance, deposited as NCIMB41658, PCT publication no. WO2006 / 108674, U.S. patents no. 8,012,689 and 9,322,069, and USDA-APHIS petition 96-068-0 lp, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0147] · A2704-21 (also known as Liberty Link® soybeans for glufosinate herbicide resistance, and the determination of the uncontrolled status of transgenic glufosinate-resistant soybean (GRS) lines W62, W98, A2704-12, A2704-21, and A5547-35 is described in USDA-APHIS Petition 96-068-0 lp, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0148] · A5547-127 (also known as Liberty Link® soybeans for EE_GM2, LL55, and glufosinate herbicide resistance, deposited as NCIMB 41660, PCT publication no. WO2006 / 108675, U.S. patents no. 8,017,756 and 8,952,142, and USDA-APHIS petition 98-014-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0149] · EE-GM4 (for nematode resistance, deposited as ATCC PTA-123624, described in PCT Publication No. WO 2018 / 119361 and U.S. Patent No. 11,242,539, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0150] · A5547-35 (also known as Liberty Link® for glufosinate herbicide resistance, and regarding the determination of the uncontrolled status of the transgenic glufosinate-resistant soybean (GRS) lines W62, W98, A2704-12, A2704-21, and A5547-35, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0151] · CV127 (also known as 127, culture for sulfonylurea herbicide resistance, deposited as NCIMB 41603, PCT publication no. WO2010 / 080829, U.S. patents no. 9,024,114 and 8,952,142 and 9,961848, and USDA-APHIS petition 09-015-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0152] · DAS44406-6 (also known as Enlist E3 for glufosinate, glyphosate, and 2,4-D herbicide resistance, deposited as ATCC PTA-11336, PCT Publication No. WO2012 / 075426, U.S. Patents No. 9,540,655, 10,400,250, and 11,425,906, and USDA-APHIS Petition 11-234-0lp, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0153] · DAS68416-4 (also known as Enlisted Soybean for Glufosinate and 2,4-D Herbicide Resistance, deposited as ATCC PTA-10442, and described in PCT Publication No. WO2011 / 066384, WO2011 / 066360, U.S. Patent No. 9,944,944, and USDA-APHIS Petition 09-349-0lp, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0154] · DAS81419 (for glufosinate herbicide resistance and lepidopteran insect resistance, deposited as ATCC PTA-12006, PCT Publication No. WO2013 / 016520, and U.S. Patent No. 8,632,978, USDA-APHIS Petition 12-272-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0155] · DAS14536-7 (for glyphosate, aryloxyalkanoate, and glufosinate herbicide resistance, deposited as ATCC PTA-11335, described in PCT Publication No. WO2012 / 075429 and U.S. Patent No. 9,540,656, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0156] · DAS21606-3 (also known as Event 1606 for phenoxyoxinic acid and / or aryloxyalkanoate herbicide resistance, deposited as ATCC PTA-11028, described in PCT Publication No. WO2012 / 033794 and U.S. Patent No. 8,785,728, the entire contents and disclosures of which are incorporated herein by reference in whole),

[0157] · DP356043 (also known as Optimum GAT for glyphosate and sulfonylurea herbicide resistance, deposited as ATCC PTA-8287, PCT Publication No. WO2008 / 002872, U.S. Patent No. 7,951,995, USDA-APHIS Petition 06-271-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0158] · DP305423 (also known as Treus, Plenish for quality characteristics, as described in PCT Publication No. WO2008 / 054747, U.S. Patents No. 8,609,953, 9,816,098, 10,745,711, and 11,390,875, USDA-APHIS Petition 06-354-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0159] · DBN9004 (for glyphosate and glufosinate herbicide resistance, deposited with CGMCC No. 11171 (Center for General Microbiology, Chinese Microbial Culture Collection and Management Committee) and described in PCT Publication No. WO2017 / 215328, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0160] · FG72 (also known as EE-GM3 for glyphosate and isooxaflutol herbicide resistance, deposited in NCIMB 41659, PCT publication no. WO2011 / 063411, U.S. patents no. 8,592,650, 3,642,748, 9631202 and 10,494,681, and USDA-APHIS petition 09-328-0lp, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0161] · GMB151 (also known as EE-GM5 for HPPD inhibitory herbicides and nematode resistance, deposited as ATCC PTA-123625, described in PCT Publication No. WO2018 / 119364, U.S. Patent No. 11,555,202, and USDA-APHIS Petition 19-317-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0162] · GTS 40-3-2 (40-3-2, known as Roundup Ready for glyphosate herbicide resistance and described in USDA-APHIS Petition 93-258-01 for the determination of the uncontrolled status of glyphosate-resistant soybean line 40-3-2, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0163] · GU262 (also known as Liberty Link for glufosinate herbicide resistance and described in USDA-APHIS Petition 98-238-01p, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0164] · HB4 (also known as IND-00410-5, Verdeca HB4 soybean for abiotic stress tolerance, deposited as ATCC PTA-125535, PCT Publication No. WO2020 / 197558, U.S. Patent Publication No. 2022 / 0090114, and USDA-APHIS Petition 17-223-01p and U.S. FDA BNF No. 000155, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0165] · MON87701 (for insect resistance to lepidopteran, deposited as ATCC PTA-8194, PCT Publication No. WO2009 / 064652, U.S. Patents No. 8,049,071 and 8,455,198, and USDA-APHIS Petition 09-082-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0166] · MON87705 (also known as Vistive Gold for modified product quality, deposited as ATCC PTA-9241, PCT publication no. WO2010 / 037016, U.S. patents no. 8,692,080, 8,329,989, 9,572,311, and 10,344,292, and USDA-APHIS petition 09-201-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0167] · MON87708 (for dicamba herbicide resistance in Genuity® Roundup Ready® 2 Xtend®, deposited as ATCC PTA-9670, PCT Publication No. WO2011 / 034704, U.S. Patent Nos. 9,447,428, RE46292E1, and 11,130,961, and USDA-APHIS Petition 10-188-01r, the entire contents and disclosures of which are incorporated herein by reference in their entirety),

[0168] · MON87712 (for increased yield, deposited as ATCC PTA-10296, PCT Publication No. WO2012051199, U.S. Patent Nos. 9,493,786, 10,053,704, 10,604,765, and 10,696,976, and USDA-APHIS Petition 11-202-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0169] · MON87751 (for insect resistance to lepidopteran, deposited as ATCC PTA-120166, PCT Publication No. WO2014 / 201235, U.S. Patents No. 9,719,145, 10,584,391 and 11,236,399, and USDA-APHIS Petition 13-337-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0170] · MON87769 (for quality characteristics, deposited as ATCC PTA-8911, PCT Publication No. WO2009 / 102873, U.S. Patent Nos. 8,692,076 and 8,999,411, and USDA-APHIS Petition 09-183-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0171] · MON87754 (for quality characteristics, deposited as ATCC PTA-9385, PCT Patent Publication No. WO2010 / 024976, and U.S. Patent No. 9,078,406, the entire contents and disclosures of each of which are incorporated herein by reference in their entirety),

[0172] · MON89788 (also known as Genuity® Roundup Ready 2 Yield for glyphosate herbicide resistance, deposited as ATCC PTA-6708, PCT Publication No. WO2006 / 130436, U.S. Patent Nos. 7,608,761, 7,632,985, 8,053,184, 8,754,289, 9,017,947, 9,605,272, 9,944,945, 10,273,498, 11,390,881, and USDA-APHIS Petition 06-178-01p, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0173] · Gm_CSM63714 (for resistance to dicamba, glufosinate, 2,4-D, and mesotrione herbicides, deposited as ATCC PTA-127099, described in U.S. Publication No. WO2012 / 075429 and U.S. Patent Application No. 2023 / 0348927, the entire contents and disclosures of which are incorporated herein by reference in whole),

[0174] · SYHT0H2 (also known as the herbicide-resistant soybean line for 0H2, glufosinate, and mesotrione herbicide resistance, deposited as ATCC PTA-11226, and described in PCT Publication No. WO2012 / 082548, U.S. Patents No. 10,184,134 and 11,053,514, and USDA-APHIS Petition 12-215-01r, the entire contents and disclosures of each of these are incorporated herein by reference in their entirety),

[0175] · W62 (also known as Liberty Link for glufosinate herbicide resistance, and regarding the determination of the uncontrolled status of transgenic glufosinate-resistant soybean (GRS) lines W62, W98, A2704-12, A2704-21, and A5547-35, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0176] · W98 (also known as Liberty Link for glufosinate herbicide resistance, and regarding the determination of the uncontrolled status of transgenic glufosinate-resistant soybean (GRS) lines W62, W98, A2704-12, A2704-21, and A5547-35, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0177] · 260-05 (also known as G94-1, G94-19, and G168 for modified product quality, and set forth in USDA-APHIS Petition 97-008-01p, the entire contents and disclosures thereof are incorporated herein by reference in their entirety),

[0178] · DAS81419 x DAS44406 (also known as Conkesta Enlist E3 for glufosinate, glyphosate, and 2,4-D herbicide resistance, and lepidopteran insect resistance),

[0179] · DAS68416-4 x MON89788 (also known as Enlisted RoundUp Ready® 2 soybeans for glufosinate, glyphosate, and 2,4-D herbicide resistance),

[0180] · FG72 x A5547-127 (also known as Liberty Link® GT27 for EE-GM3 x EE-GM2, glufosinate, glyphosate, and isosaflutol herbicide resistance, deposited as ATCC PTA-11042 and described in PCT Patent Publication No. WO2011 / 063413, the entire contents and disclosures of each of which are incorporated herein by reference in their entirety),

[0181] · MON87701 x MON89788 (also known as Intacta® Roundup Ready® 2 Pro for glyphosate herbicide resistance and Nadiadoptera insect resistance),

[0182] · MON87751 x MON87701 x MON87708 x MON89788 (for glyphosate and dicamba herbicide resistance and lepidopteran insect resistance),

[0183] · MON87705 x MON87708 (for dicamba herbicide resistance and modified product quality),

[0184] · MON87705 x MON89788 (also known as Visive Gold for glyphosate herbicide resistance and modified product quality),

[0185] · MON87708 x MON89788 (also known as Roundup Ready 2 Xtend soybeans for glyphosate and dicamba herbicide resistance),

[0186] · MON87705 x MON87708 x MON89788 (for glyphosate and dicamba herbicide resistance, and modified product quality),

[0187] · MON87708 x MON89788 x A5547-127 (for glufosinate, glyphosate, and dicamba herbicide resistance),

[0188] · MON87769 x MON89788 (known as Omega-3 X Genuity Roundup Ready 2 yield soybeans for glyphosate herbicide resistance and modified product quality),

[0189] · HB4 x GTS 40-3-2 (for glyphosate herbicide resistance and abiotic stress resistance),

[0190] · DP305423 x GTS 40-3-2 (for glyphosate sulfonylurea herbicide resistance, and modified product quality),

[0191] · DP305423 x MON87708 (for dicamba herbicide resistance and modified product quality),

[0192] · P305423 x MON87708 x MON89788 (for glyphosate and dicamba herbicide resistance, and modified product quality),

[0193] · DP305423 x MON89788 (for glyphosate herbicide resistance, and modified product quality),

[0194] As used herein, "Gm_CSM63714" refers to the soybean event Gm_CSM63714. Soybean seeds containing the event Gm_CSM63714 are deposited under ATCC accession number PTA-127099 and are fully described and characterized in U.S. Patent Application Publication No. 2023 / 0348927, the entire contents and disclosures of which are incorporated herein by reference in whole. Transgenic soybean plants containing the soybean event Gm_CSM63714 comprise SEQ ID NO. 182 (5' soybean genome lateral sequence + transgenic insert + 3' soybean genome lateral sequence), SEQ ID NO. 183 (transgenic insert), SEQ ID NOs 184-187 (5' junction sequence), and SEQ ID NOs 188-191 (3' junction sequence). The transgenic insert of a soybean plant containing event Gm_CSM63714 comprises four expression cassettes as shown in Table 2. The first expression cassette is in an operable connection (i) Arabidopsis thaliana The promoter, leader, and intron sequences of the ubiquitin (UB3) gene, (ii) Arabidopsis thaliana (iii) the APG6 (Albino and Pale Green 6) chloroplast transfer peptide sequence, (iii) the codon-optimized sequence encoding the dicamba monooxygenase (DMO) variant of Stenotrophomonas maltophilia, and (iv) Medicago Trunkatula The second expression cassette comprises the 3' UTR sequence of the aluminum-derived Sali3-2; and in an operable linkage, i) a plurality of Arabidopsis thaliana (ii) a promoter sequence derived from a promoter sequence, (ii) a plurality of Arabidopsis thaliana Intron sequence derived from an intron sequence, (iii) Streptomyces viridochromogenesThe codon-optimized coding sequence of phosphinothricin N-acetyltransferase (PAT), and (iv) Medicago Trooncatula It contains the 3' UTR sequence of a small heat shock protein (Hsp20); and the third expression cassette in an operable linkage (i) Arabidopsis thaliana The promoter, leader, and intron sequences of the polyubiquitin gene (UBQ10), (ii) Sphingobium herbicidoborans A codon-optimized sequence encoding an alpha-ketoglutarate-dependent non-heme iron dioxygenase variant of, and (iii) Medicago Trunkatula It includes the 3' UTR sequence of the putative protein of; and the fourth expression cassette in an operable linkage (i) a plurality of Arabidopsis thaliana (ii) a promoter sequence derived from a promoter sequence, (ii) a plurality of Arabidopsis thaliana 5' UTR leader sequence derived from a 5' UTR sequence, (iii) a plurality of Arabidopsis thaliana Intron sequence derived from an intron sequence, (iv) Oriza Sativa A codon-optimized sequence encoding triketone dioxygenase in, (v) a plurality of Zea Mays It includes a 3' UTR sequence derived from a 3' UTR sequence.

[0195]

[0196] Soybean plants containing event Gm_CSM63714 exhibit resistance to benzoate auxins such as dicamba, glutamine synthase inhibitors such as glufosinate; phenoxyauxins such as 2,4-D, and β-triketone herbicides such as mesotrione (4-hydroxyphenylpyruvate dioxygenase or HPPD inhibitors).

[0197] As used herein, "MON89788" refers to soybean event MON89788. Soybean seeds containing event MON89788 have been deposited under ATCC accession number PTA-6708 and are fully described and characterized in PCT publication number WO2006 / 130436 and U.S. patents number 7,608,761, 7,632,985, 8,053,184, 8,754,289, 9,017,947, 9,605,272, 9,944,945, 10,273,498, 11,390,881, the entire contents and disclosures of which are incorporated herein by reference in their entirety. Transgenic soybean plants containing soybean event MON89788 comprise SEQ ID NO. 192 (5' soybean genome lateral sequence + transgenic insert + 3' soybean genome lateral sequence), SEQ ID NO. 193 (transgenic insert), SEQ ID NOs 194-195 (5' junction sequence), and SEQ ID NOs 196-197 (3' junction sequence). The transgenic insert in soybean plants containing event MON89788 comprises operable linkages of (i) the chimeric promoter FMV / Tsfl and the associated linked element (referred to as FMV / ElFlα), and (ii) Arabidopsis EPSPS chloroplast transfer peptide coding sequence (referred to as CTP2 or TS-AtEPSPS CTP2), (iii) glyphosate-resistant EPSPS coding sequence (modified for enhanced expression in plant cells Agrobacterium thumefaciens (iv) a CP4 EPSPS expression cassette containing a 3' UTR from pea ribulose 1,5-bisphosphate carboxylase (referred to as E9 3' or T-Ps.RbcS:E9) from the CP4 codon of the strain, referred to as CP4 EPSPS or aroA:CP4. Soybean plants containing event MON89788 exhibit resistance to glyphosate herbicides.

[0198] Any of the soybean plants, plant parts, seeds, cells, offspring, or commercial products described herein containing soybean event Gm_CSM63717 may additionally contain soybean event Gm_CSM63714 and / or MON89788.

[0199] Any of the soybean plants, plant parts, seeds, cells, offspring or commercial products described herein are SEQ ID NO. 182; SEQ ID NO. 183; SEQ ID NO. 184; SEQ ID NO. 185; SEQ ID NO. 186; SEQ ID NO. 187; SEQ ID NO. 188; SEQ ID NO. 189; SEQ ID NO. 190; SEQ ID NO. 191; SEQ ID NO. 192; SEQ ID NO. 193; SEQ ID NO. 194; SEQ ID NO. 195; SEQ ID NO. 196; It may further comprise a polynucleotide having a sequence selected from the group consisting of SEQ ID NO. 197, the entire length of SEQ ID NO. 182 or the entire length of SEQ ID NO. 183 or the entire length of SEQ ID NO. 192 or the entire length of SEQ ID NO. 193 that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the sequence, and a complete complement of any of the above.

[0200] Plants containing soybean event Gm_CSM63717 and soybean event Gm_CSM63714 and / or soybean event MON89788 can be produced by any method known to the art. For example, plants containing soybean event Gm_CSM63717 and soybean event Gm_CSM63714 or soybean event MON89788 can be produced by crossing a soybean plant containing soybean event Gm_CSM63717 with a soybean plant containing soybean event Gm_CSM63714 or soybean event MON89788 and selecting offspring plants containing both Gm_CSM63717 and Gm_CSM63714 or offspring plants containing both Gm_CSM63717 and MON89788. A plant containing soybean events Gm_CSM63717, Gm_CSM63714, and MON89788 can be produced, for example, by crossing a soybean plant containing event Gm_CSM63717 with a soybean plant containing both Gm_CSM63714 and MON89788, or by first crossing a soybean plant containing event Gm_CSM63714 with a soybean plant containing event MON89788 and selecting a offspring plant containing both Gm_CSM63714 and MON89788; and then crossing the offspring plant containing both Gm_CSM63714 and MON89788 with a plant containing event Gm_CSM63717 and selecting an offspring plant containing Gm_CSM63714, MON89788, and Gm_CSM63717.Alternatively, a soybean plant containing soybean event Gm_CSM63717 is first crossed with a soybean plant containing event Gm_CSM63714 (or event MON89788), and a plant of offspring containing both soybean events Gm_CSM63717 and Gm_CSM63714 (or MON89788) is selected, and then the plant of offspring containing both Gm_CSM63717 and Gm_CSM63714 (or MON89788) is crossed with a plant containing event MON89788 (or Gm_CSM63714), and a plant of offspring containing all three events can be selected.

[0201] The term "site-specific nuclease" refers to any enzyme capable of cleaving nucleotide sequences in a site-specific manner. Site-specific nucleases enable precise and / or targeted editing at specific locations in the plant genome. Site-specific nucleases include, for example, RNA-guided nucleases, zinc finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs).

[0202] Some site-specific nucleases, such as zinc finger nucleases (ZFNs) and TALENs, are not RNA-guided but instead rely on their protein structure to determine their target site for causing DSBs (double-strand breaks) or nicks, or are fused, tethered, or attached to DNA-binding protein domains or motifs. The protein structure of the site-specific nuclease (or the fused / attached / tethered DNA-binding domain) targets the site-specific nuclease to a target site. ZFNs and TALENs can be designed, manipulated, and constructed according to known methods for targeting and binding to target sites.

[0203] RNA-guided nucleases form a complex with guide RNA ( for exampleIt is a nuclease that forms a complex (a ribonucleoprotein) and then guides the complex to a target site within a target sequence. One non-limiting example of a guided nuclease is the CRISPR nuclease. CRISPR (clustered, regularly interspersed short palindromic repeats) nucleases are proteins found in bacteria that are guided to target nucleic acid molecules by guide RNA ("gRNA"), where the endonuclease can cleave the target nucleic acid molecule into one or two strands. Although the source of CRISPR nucleases is bacteria, many CRISPR nucleases have been shown to function in eukaryotic cells. A CRISPR editing system comprising a CRISPR-associated protein (nuclease) and a homologous guide RNA (which can be transcribed from a guide DNA polynucleotide) can be used for targeted DNA cleavage or modification. CRISPR-associated proteins are type I CRISPR-associated proteins, type II CRISPR-associated proteins, type III CRISPR-associated proteins, type IV CRISPR-associated proteins, type V CRISPR-associated proteins, or type VI CRISPR-associated proteins, e.g., Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Cas12a (also known as Cpf1), Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, It can be selected from Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, CasX, CasY, and Mad7.

[0204] As further described in Example 2 below, the soybean event Gm_CSM63717 was incorporated into the genome at a site close to the location of event Gm_CSM63714. To achieve this, bioinformatics analysis was first used to identify genomic target sites for site-specific integration (SDI) of the transformed gene.

[0205] As used herein, the terms “target site,” “genome target site,” “target genomic nucleic acid,” or “target soybean genome nucleic acid” refer to a polynucleotide sequence sufficiently unique in the soybean genome that allows targeted genomic modification by site-specific nucleases. In one aspect, the sequence of the target site is altered from the wild-type sequence, that is, the target site is edited. In another aspect, the target site is an insertion site of the DNA sequence of interest.

[0206] The target site may include one or more criteria selected from the group consisting of (i) the selected target site is greater than 2 kb from the gene, (ii) the selected target site is greater than 1,000 nucleotides (nt) from the small RNA hotspot, and (iii) the target site contains 700 nucleotides of a unique sequence on both sides, and the criteria for selecting the target site are further described in U.S. Patent Application Publication No. 2020 / 0024610 and PCT Publication No. WO 2024 / 129512, the entire contents and disclosures of which are incorporated herein by reference in their entirety.

[0207] The target site comprises a sequence recognized by a site-specific nuclease. In some embodiments, the target site comprises a sequence recognized by a site-specific nuclease that results in precise or targeted cleavage within the target site. For example, the site-specific nuclease may be selected from the group consisting of RNA-guided nucleases, zinc finger nucleases, and TALENs. In some embodiments, the target site comprises a PAM (protospacer adjacent motif) sequence recognized by an RNA-guided nuclease (e.g., a CRISPR nuclease system). For example, the target site may comprise a PAM motif recognized by a Cas12a / Cpf1 CRISPR nuclease system. The target site may further comprise a sequence recognized and hybridized by a CRISPR guide RNA. In some embodiments, the target site comprises a sequence recognized and hybridized by a Cas12a / Cpf1 CRISPR guide RNA.

[0208] The DNA sequence of interest may be inserted into a target site using a site-specific nuclease. As used herein, the terms “DNA sequence of interest,” “donor sequence,” or “donor DNA” refer to a nucleic acid / DNA sequence selected for targeted insertion into a soybean genome sequence. In one aspect, the soybean genome sequence is the genomic target site described above. The DNA sequence of interest may be of any length, for example, 2 to 50,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 1,000 to 5,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 5,000 to 10,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 10,000 to 15,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 15,000 to 20,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 20,000 to 25,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 25,000 to 30,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 30,000 to 35,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 35,000 to 40,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 40,000 to 45,000 nucleotide lengths (or any integer value in between). In some embodiments, the DNA sequence is about 45,000 to 50,000 nucleotide long (or any integer value in between).The DNA sequence may comprise one or more gene expression cassettes that further comprise actively transcribed and / or translated gene sequences. For example, the DNA sequence of interest may comprise a gene expression cassette comprising a herbicide resistance gene, an insecticide resistance gene, a nitrogen use efficiency gene, a water use efficiency gene, a nutrient quality gene, a DNA binding gene, a selectable marker gene, a target site for a site-specific nuclease, and any combination thereof. Alternatively, the DNA sequence of interest may comprise a polynucleotide sequence that does not contain a functional gene expression cassette or the entire gene (e.g., may include regulatory sequences such as promoters, enhancers, etc.), or may not contain any identifiable gene expression elements or any actively transcribed gene sequences. In some embodiments, the DNA of interest will have at least one homologous arm DNA sequence. The term "homologous arm DNA sequence" refers to a polynucleotide sequence having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with respect to a target sequence of a plant or plant cell. Additionally, the DNA sequence may be linear or circular, and may be single-stranded or double-stranded. It may be delivered to the cell as a naked nucleic acid, as a complex with one or more delivery agents (e.g., liposomes, poloxamers, protein-encapsulated T-strands, etc.), or, for example, each. Agrobacterium thumefaciens Alternatively, it can be contained in bacterial or viral delivery vehicles such as Gemini viruses or nanoviruses.

[0209] Once a specific target site is identified, a site-specific nuclease targeting the selected target site can be designed and introduced into a plant, seed, or plant cell. For example, a CRISPR-associated nuclease (e.g., Cas12a / Cpf1) and at least one RNA guide molecule capable of hybridizing to the target site can be designed, cloned into a plant expression vector, and delivered to a plant, seed, or plant cell. If the genomic modification is designed to induce double-strand breaks (DSBs) with non-homologous end-junction (NHEJ) repair for the introduction of insertions and deletions (indels) (i.e., to induce cleavage), only the engineered CRISPR nuclease and at least one RNA guide molecule are delivered to the plant, seed, or cell. If the DNA sequence of interest is to be incorporated into the target site, the engineered CRISPR nuclease, at least one RNA guide molecule, and the DNA of interest are co-delivered to the plant, seed, or cell. The DNA of interest can be incorporated into the target site by NHEJ (non-homologous end binding) or homology-dependent repair (HR). In the latter case, the DNA of interest will have at least one homologous subarm DNA sequence. An alternative to delivering engineered CRISPR nucleases as DNA expression constructs is the delivery of ribonucleoprotein (RNP) complexes of CRISPR-associated nuclease proteins as complexes with guide RNA.

[0210] After delivering site-specific nucleases to plant cells, the cells or plants regenerated from the cells are sampled to confirm the presence of intended site-specific genomic modifications, including the insertion of DNA sequences of interest at or near the target site. Methods for detecting genomic modifications are known to those skilled in the art and include PCR, TaqMan® PCR, droplet digital PCR (ddPCR, Bio-Rad Laboratories, Hercules, California), sequencing, Sanger sequencing, ABI 3730 DNA fragment analysis (Applied Biosystems, Grand Island, New York), Southern analysis, Northern analysis, phenotypic analysis, or any other technique known to those skilled in the art for detecting genomic modifications.

[0211] As further described in Example 2 below, 12 target sites were identified within 5 centimorgans (cM) upstream and downstream of event Gm_CSM63714. The sequences of these target sites are provided herein as SEQ ID NOs 211-222. Guide RNA spacer sequences corresponding to each target site sequence are provided as SEQ ID NOs 223-234.

[0212] A soybean plant, a plant seed, a plant part, a plant cell, and a progeny plant are provided. The plant, seed, plant part, plant cell, or progeny plant comprises a recombinant nucleic acid molecule. The recombinant nucleic acid molecule comprises a target soybean genome nucleic acid sequence having at least 85% sequence identity, at least 90% sequence identity, or at least 95% sequence identity with respect to a nucleic acid molecule selected from the group consisting of SEQ ID NOs 211–222. The recombinant nucleic acid molecule further comprises a DNA sequence of interest. The DNA sequence of interest is inserted into the target soybean genome nucleic acid sequence. In some embodiments, the plant, seed, plant part, or progeny plant comprises a recombinant nucleic acid molecule comprising a target soybean genome nucleic acid sequence having a sequence selected from the group consisting of SEQ ID NOs 211–222.

[0213] The DNA sequence of interest may include agronomic genes of interest. For example, agronomic genes of interest can confer herbicide resistance in plants, such as the PPO gene that confers resistance to PPO herbicides.

[0214] In some embodiments, the target soybean genome nucleic acid sequence is at least 1 kb from the Gm_CSM63714 insertion site. In some embodiments, the target soybean genome nucleic acid sequence is mapped within 3.8 cM of the Gm_CSM63714 insertion site. In some embodiments, the target soybean genome nucleic acid sequence is more than 2 kb from the gene, more than 1,000 nucleotides from the small RNA hotspot, and includes 700 nucleotides of a sequence unique to both sides.

[0215] A method for generating recombinant soybean plant cells is provided. The method comprises: a) obtaining a soybean plant, seed, or cell, wherein the plant, seed, or cell comprises a target soybean genome nucleic acid molecule having at least 85% sequence identity, at least 90% sequence identity, or at least 95% sequence identity with respect to a nucleic acid molecule selected from the group consisting of SEQ ID NOs 211-222; b) introducing a site-specific nuclease capable of specifically binding to and cleaving the target soybean genome nucleic acid molecule into the soybean plant, seed, or cell; c) introducing a DNA sequence of interest into the soybean plant, seed, or cell; and d) selecting a recombinant soybean plant, seed, or cell comprising the DNA sequence of interest inserted into the target soybean genome nucleic acid molecule. The site-specific nuclease may be selected from the group consisting of RNA-guided nucleases, zinc finger nucleases, and TALENs. For example, the RNA-guided nuclease may be Cas12a. The method may further include the step of introducing a guide polynucleotide comprising a nucleic acid sequence substantially complementary to the target soybean genome nucleic acid into a soybean plant, seed, or cell, wherein the guide polynucleotide and the RNA-guided nuclease form a complex capable of binding to and cleaving the soybean genome nucleic acid molecule. The guide polynucleotide may comprise a nucleotide sequence having at least 85% sequence identity, at least 90% sequence identity, or at least 95% sequence identity with respect to a nucleic acid molecule selected from the group consisting of SEQ ID NOs 223-234. The guide polynucleotide may further comprise SEQ ID NO. 207. In some embodiments, the target soybean genome nucleic acid sequence is at least 1 kb from the Gm_CSM63714 insertion site. In some embodiments, the target soybean genome nucleic acid sequence is mapped within 3.8 cM of the Gm_CSM63714 insertion site.In some embodiments, the target soybean genome nucleic acid sequence is greater than 2 kb from the gene, greater than 1,000 nucleotides from the small RNA hotspot, and includes 700 nucleotides of a unique sequence on both sides.

[0216] A recombinant DNA molecule is provided. The recombinant nucleic acid molecule comprises a DNA sequence having at least 85% sequence identity, at least 90% sequence identity, or at least 95% sequence identity with respect to a nucleic acid molecule selected from the group consisting of SEQ ID NOs 211-222. For example, the nucleic acid molecule may be selected from the group consisting of SEQ ID NOs 211-222. The DNA sequence may be operably linked to a heterogeneous promoter sequence. The recombinant DNA molecule may further comprise SEQ ID NO 207.

[0217] A recombinant RNA molecule is provided. The recombinant RNA molecule comprises an RNA sequence that is at least 85% complementary, at least 90% complementary, or at least 95% complementary to a nucleic acid molecule selected from the group consisting of SEQ ID NOs 211-222. In some embodiments, the RNA sequence is 100% complementary to a nucleic acid molecule selected from the group consisting of SEQ ID NOs 211-222.

[0218] The plants described herein may be used to produce offspring or descendants containing the soybean event Gm_CSM63717. These offspring may comprise any plant, seed, and cell and / or regenerable plant part containing soybean event Gm_CSM63717 derived from or inherited from an ancestral or parental soybean plant(s), at least one of which is at least one polynucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, a polynucleotide comprising at least 16 consecutive nucleotides of SEQ ID NO. 1, at least 16 consecutive nucleotides of SEQ ID NO. 2, at least 33 consecutive nucleotides of SEQ ID NO. 3, at least 32 consecutive nucleotides of SEQ ID NO. 4, at least 53 consecutive nucleotides of SEQ ID NO. 5, or at least 52 consecutive nucleotides of SEQ ID NO. 6, or the entire length of SEQ ID NO. 10 or the entire length of SEQ ID NO. 9 and at least 90%, at least 91%, at least It comprises a DNA molecule having or containing a polynucleotide having the same nucleotide sequence as 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9%.

[0219] Soybean plants, offspring, and seeds may be homozygous or heterozygous for event Gm_CSM63717 and the transforming gene of event Gm_CSM63717. Offspring are seeds produced by soybean plants containing or including event Gm_CSM63717 and / or fertilized with pollen of soybean plants containing or including event Gm_CSM63717 (i.e., event Gm_CSM63717It can be grown from seeds produced by plants (modified with pollen containing or including). Plants or offspring can also be obtained by tissue culture and regeneration methods from protoplasts, cells, embryos, or reproductive or somatic tissues derived from soybean plants containing or including soybean event Gm_CSM63717.

[0220] Progeny plants can be self-pollinated (also known as "self-fertilization") to produce a true breeding line of plants, namely plants that are homozygous for the soybean event Gm_CSM63717 DNA. Alternatively, progeny plants can be crossbred, that is, bred with another plant to produce variety or hybrid seeds or plants. The other plant may be transgenic or non-transgenic. Thus, the variety or hybrid seeds or plants of this disclosure may be derived by crossing a first parent lacking the specific and unique DNA of event Gm_CSM63717 with a second parent containing event Gm_CSM63717 to produce a hybrid containing the specific and unique DNA of event Gm_CSM63717. Each parent is crossbred or bred with the plant or seed of the present disclosure, i.e., the specific and unique DNA of event Gm_CSM63717 and / or at least one allele comprising at least 16 consecutive nucleotides of SEQ ID NO. 1, at least 16 consecutive nucleotides of SEQ ID NO. 2, at least 33 consecutive nucleotides of SEQ ID NO. 3, at least 32 consecutive nucleotides of SEQ ID NO. 4, at least 53 consecutive nucleotides of SEQ ID NO. 5, or at least 52 consecutive nucleotides of SEQ ID NO. 6, or the whole length of SEQ ID NO. 10 or the whole length of SEQ ID NO. 9 and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least Insofar as it produces seeds having polynucleotides with 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical sequences, it may be a hybrid or an inbreed / variety.

[0221] Sexual cross-pollination of one plant with another, that is, cross-pollinating, can be achieved or facilitated by human intervention, for example: collecting pollen from one plant by human hands and bringing this pollen into contact with the style or stigma of a second plant; removing, destroying, or covering the stamens or anthers of the plant by human hands and / or actions (e.g., manual intervention or application of chemical gametes) to prevent natural self-pollination and for cross-pollination to occur; placing pollinating insects in a location for "directed pollination" (e.g., placing beehives in orchards or fields, or confining plants with pollinating insects); opening or removing parts of a flower to place or bring foreign pollen into contact with the style or stigma; selectively placing plants (e.g., intentionally sowing plants near pollinators); or applying chemicals to trigger flowering or create receptivity (of the stigma to pollen).

[0222] Therefore, by crossing two different transgenic plants of the same or different genetic backgrounds, it is possible to produce inbred or hybrid offspring plants, plant parts, and / or seeds containing transgenic genes or events that separate independently into two, wherein at least one of these transgenic genes or events is contained or included within the soybean event Gm_CSM63717. For example, a transgenic plant containing the soybean event Gm_CSM63717 can be crossed with another transgenic soybean plant to produce a plant having the characteristics of both transgenic parents.

[0223] Backcrossing to parent plants and crossbreeding with non-transformed plants are also considered, as is vegetative propagation. Descriptions of other breeding methods commonly used for different traits and crops are known in the art and in one of several references, for example , Fehr, in Breeding Methods for Cultivar Development It can be found in Wilcox J. ed., American Society of Agronomy, Madison WI (1987).

[0224] A plant part is provided. As used herein, “plant part” refers to any part of a plant composed of material directly from or derived from a plant containing the soybean event Gm_CSM63717. The plant part includes, but is not limited to, microspores, pollen, anthers, ovules, ovaries, flowers, pods, embryos, buds, nodes, stems, leaves, roots, and callus tissue, in whole or in part. The plant part may be viable or non-viable. The plant part may be regenerable or non-regenerable.

[0225] Inactive or non-renewable soybean plant material is provided herein. The inactive or non-renewable soybean plant material may comprise any of the characteristic recombinant DNA molecules of soybean event Gm_CSM63717 described herein, or any of the DNA constructs described herein. The inactive or non-renewable soybean plant material may comprise soybean event Gm_CSM63717, which is a representative sample of seeds containing soybean event Gm_CSM63717 deposited under ATCC accession number PTA-127604.

[0226] A commercial product is provided comprising any of the characteristic DNA molecules of the soybean event Gm_CSM63717 or any of the DNA constructs described herein. Such commercial product may be produced from a plant containing the soybean event Gm_CSM63717. The product contains a detectable amount of DNA comprising a DNA sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, or a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the entire length of SEQ ID NO. 10 or the entire length of SEQ ID NO. 9. As used herein, “product” refers to any composition or product consisting of material of a plant, seed, cell, or plant part containing the soybean event Gm_CSM63717. The product may be viable or non-living plant material, i.e., material derived from a non-living plant, seed, cell, or plant part containing the soybean event Gm_CSM63717.Non-viable product products include, but are not limited to, non-viable seeds, whole or processed seeds, processed plant tissue or plant parts, dehydrated plant tissue or parts, frozen plant tissue or parts, foods for human consumption such as soybean oil, soy milk, soybean flour and soybean kernels, soybean protein, soybean protein concentrate, hydrolyzed vegetable protein, meat substitutes made from soybean protein, lecithin, curd, tofu, vegetable soybeans (green soybeans), bean sprouts, soybean film (yuba), roasted soybeans, miso, tempeh, soy sauce or natto, plant parts processed into animal feed such as soybean meal, soybean fiber, biodiesel, bio-composite building materials (e.g., particleboard, laminated plywood and wood products), soybean oil-based solvents, soybean oil-based commercial lubricants, soybean ink, soybean candles, soybean crayons, soybean-based hydraulic fluids, or soybean-based foams. Viable product products include, but are not limited to, viable seeds, viable plant parts (e.g., nodes, buds, roots, and leaves), and viable plant cells. Accordingly, plants containing event Gm_CSM63717 can be used to manufacture any product product typically obtained from soybean plants.Any of these product products derived from a plant containing event Gm_CSM63717 may contain at least a detectable amount of specific and unique DNA corresponding to event Gm_CSM63717, specifically a detectable amount of a polynucleotide having a nucleotide sequence of at least 16 consecutive nucleotides of SEQ ID NO. 1, at least 16 consecutive nucleotides of SEQ ID NO. 2, at least 33 consecutive nucleotides of SEQ ID NO. 3, at least 32 consecutive nucleotides of SEQ ID NO. 4, at least 53 consecutive nucleotides of SEQ ID NO. 5, or at least 52 consecutive nucleotides of SEQ ID NO. 6, or the whole length of SEQ ID NO. 10 or the whole length of SEQ ID NO. 9 and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, or at least 99.2%, It may contain polynucleotides having at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical sequences. Any standard detection method for polynucleotide molecules, including the detection method disclosed herein, may be used.

[0227] A method for producing such a product is also provided. The method comprises: (a) obtaining a transgenic soybean plant, plant part, or plant seed containing the soybean event Gm_CSM63717; and (b) producing a product from the transgenic soybean plant, plant part, or plant seed.

[0228] Plants resistant to herbicides can be produced by sexually crossing a plant containing event Gm_CSM63717 with another plant to produce seeds, and then culturing them into offspring plants. For example, a method for producing offspring soybean plants containing event Gm_CSM63717 is provided herein, the method comprising: (a) sexually crossing a first soybean plant containing soybean event Gm_CSM63717 with itself or a second soybean plant; (b) collecting one or more seeds produced by the cross; (c) culturing one or more seeds to produce one or more offspring plants; and d) selecting at least one first offspring plant or seed containing soybean event Gm_CSM63717. Inbreed and hybrid soybean plants containing soybean event Gm_CSM63717 produced by this method are also provided.

[0229] Progeny plants were analyzed using diagnostic methods to identify progeny plants containing event Gm_CSM63717 DNA, or flumioxazine, epirifenacil (also referred to as S-3100 or rapidicil; IUPAC name: ethyl [(3-{2-chloro-5-[3,6-dihydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)pyrimidine-1(2H)-yl]-4-fluorophenoxy}-2-pyridyl)oxy]acetate), lactophen, asifluorophene, pyraflufen, pyraflufen-ethyl, oxadiazone, butafenacil, 피리딘-2-일메틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-시아노-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 시아노메틸 [(3-{2-브로모-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 사이클로프로필메틸(2-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}페녹시)아세테이트,Progeny plants resistant to PPO herbicides such as methyl(2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoxymasil), fomesafene, ciflufenacil, sulfentrazone, thiafenasil, and trifludimoxazine, and any combination thereof may be selected. Other plants used may or may not be transformed. The produced progeny plants and / or seeds may be variant or hybrid seeds.

[0230] Plants resistant to PPO herbicides are a polynucleotide having the nucleotide sequences of SEQ NOs. 1-10, at least 16 consecutive nucleotides of SEQ NO. 1, at least 16 consecutive nucleotides of SEQ NO. 2, at least 33 consecutive nucleotides of SEQ NO. 3, at least 32 consecutive nucleotides of SEQ NO. 4, at least 53 consecutive nucleotides of SEQ NO. 5, or at least 52 consecutive nucleotides of SEQ NO. 6, or the whole length of SEQ NO. 10 or the whole length of SEQ NO. 9 and at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least It can be produced by self-pollinating a plant containing event Gm_CSM63717 containing a polynucleotide having 99.7%, at least 99.8%, or at least 99.9% identical sequence, thereby producing seeds, and then culturing them into offspring plants.Then, these progeny plants were analyzed using diagnostic methods to identify progeny plants containing event Gm_CSM63717 DNA, or flumioxazine, epirifenacil (also referred to as S-3100 or lapidicil; IUPAC name: ethyl [(3-{2-chloro-5-[3,6-dihydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)pyrimidine-1(2H)-yl]-4-fluorophenoxy}-2-pyridyl)oxy]acetate), lactophen, asifluorophen, pyraflufen, pyraflufen-ethyl, oxadiazone, butafenacil, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyclopropylmethyl(2-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate, methyl(2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate(. 플루페녹시마실) , fomesafen, ciflufenacil, sulfentrazone, thiafenacil, trifludimoxazine, and any combination thereof may be selected for offspring plants resistant to PPO herbicides.

[0231] Soybean Event Gm_CSM63717 is flumioxazine, epirifenacil (also referred to as S-3100 or lapidicil; IUPAC name: ethyl [(3-{2-chloro-5-[3,6-dihydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)pyrimidine-1(2H)-yl]-4-fluorophenoxy}-2-pyridyl)oxy]acetate), lactophen, asifluorophen, pyraflufen, pyraflufen-ethyl, oxadiazone, butafenacil, Pyridine-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyclopropylmethyl(2-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate, methyl It contains a PPO expression cassette that provides resistance to PPO herbicides such as (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoxymasil), fomesafene, ciflufenacil, sulfentrazone, tiafenacil, and trifludimoxazine, and any combination thereof. The soybean plants, offspring, and seeds of event Gm_CSM63717 may contain one or more additional desirable traits, such as resistance to glutamine synthase inhibitors such as glufosinate, benzoate auxin such as dicamba, phenoxyauxin such as 2,4-D, 4-hydroxyphenylpyruvate dioxygenase or HPPD inhibitors such as mesotrione, EPSPS inhibitors such as glyphosate, or any combination thereof.

[0232] PPO herbicides include diphenyl ethers, N-phenylphthalimide, oxadiazole, oxazolidinedione, phenylpyrazole, pyrimidineedione, thiadiazole, triazolinone, benzoxazinone derivatives, other PPO herbicides, and any combination thereof. Examples of diphenyl ethers include, but are not limited to, asifluorophene, bifennox, ethoxyphene, fluorodiphene, fluoronitrophene, furyloxyphene, halosafene, clomethoxyphene, chlornitrophene, ethoxyphene-ethyl, fluoroglycopene, lactophene, nitrophene, oxyfluorophene, fomesafene, any salts thereof, and any esters thereof. Examples of N-phenylphthalimide include, but are not limited to, sinidone-ethyl, flumiclorac, flumiclorac-pentyl, and flumioxazine. Examples of oxadiazoles include, but are not limited to, oxadiagils and oxadiazones. Examples of oxazolidinediones include, but are not limited to, pentoxazones. Examples of phenylpyrazoles include, but are not limited to, fluazolates, piraflufen, and piraflufen-ethyl. Examples of pyrimidindiones or phenyluracils include, but are not limited to, benzfenidizone, butafenacil, epirifenacil, flupropacil, flufenoximilm, saflufenacil, and thiafenacil. Examples of thiadiazoles include, but are not limited to, fluthiacet-methyl and thidiazimin. Examples of triazolinones include, but are not limited to, azafenidine, bencarbazone, carpentrazone, their salts and esters, and sulfentrazone. Examples of benzoxazinone derivatives include, but are not limited to, 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3,4-dihydro-3-oxo-4-prop-2-inyl-2H-1,4-benzoxazine-6-yl)-1,3,5-triazinan-2,4-dione (trifludimoxazine)). Examples of other PPO herbicides include, but are not limited to, chlorphthalim, flufenpyr, flufenpyr-ethyl, flumipropin, pyraclonil, and profluazole. Additional examples of other PPO herbicides include the following:

[0233] 1) A herbicidally active compound of general formula (I) or an agrochemically acceptable salt thereof

[0234]

[0235] (during the meal,

[0236] R 1 It is hydrogen;

[0237] R 2 is hydrogen, fluorine, chlorine, bromine, trifluoromethyl, methoxy, ethoxy, prop-1-yloxy, or but-1-yloxy, and

[0238] R 3 It is hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1-yloxy, and

[0239] R 4 is fluorine, chlorine, bromine, cyano, NO2, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethinyl, propin-1-yl, 1-butyne-1-yl, pentyne-1-yl, or hexine-1-yl, and

[0240] R 5 , R 6 and R 7is independently hydrogen, fluorine, chlorine, bromine, iodine, cyano, methyl, ethyl, prop-1-yl, 1-methylethyl, but-1-yl, 1-methylpropyl, 2-methylpropyl, 1,1-dimethylethyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl, 1-ethyl-2-methylpropyl, trifluoromethyl, difluoromethyl, pentafluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, 1,1-dimethyls-1-yloxy, difluoromethoxy, trifluoromethoxy, pentafluoroethoxy, 2,2-difluoroethoxy, or 2,2,2-trifluoroethoxy, and

[0241] G is methylene, (methyl)methylene, (ethyl)methylene, (prop-1-yl)methylene, (prop-2-yl)methylene, (but-1-yl)methylene, (but-2-yl)methylene, (pent-1-yl)methylene, (pent-2-yl)methylene, (pent-3-yl)methylene, (dimethyl)methylene, (diethyl)methylene, ethylene, n-propylene, (1-methyl)ethyl-1-en, (2-methyl)ethyl-1-en, n-butylene, 1-methylpropyl-1-en, 2-methylpropyl-1-en, 3-methylpropyl-1-en, 1,1-dimethylethyl-1-en, 2,2-dimethylethyl-1-en, 1-ethylethyl-1-en, 2-ethylethyl-1-en, 1-(prop-1-yl)ethyl-1-en, 2-(prop-1-yl)ethyl-1-en, 1-(prop-2-yl)ethyl-1-en, 2-(prop-2-yl)ethyl-1-en, 1,1,2-trimethylethyl-1-en, 1,2,2-trimethylethyl-1-en, 1,1,2,2-tetramethylethyl-1-en, n-pentylene, 1-methylbutyl-1-en, 2-methylbutyl-1-en, 3-methylbutyl-1-en, 4-methylbutyl-1-en, 1,1-dimethylpropyl-1-en, 2,2-dimethylpropyl-1-en, 3,3-dimethylpropyl-1-en, 1,2-dimethylpropyl-1-en, 1,3-dimethylpropyl-1-en, 1-ethylpropyl-1-en, n-hexylene, 1-methylpentyl-1-en, 2-methylpentyl-1-en, 3-methylpentyl-1-en, 4-methylpentyl-1-en, 1,1-dimethylbutyl-1-en, 1,2-dimethylbutyl-1-en, 1,3-di-methylbutyl-1-en, 2,2-dimethylbutyl-1-en, 2,3-dimethylbutyl-1-en, 3,3-dimethylbutyl-1-en, 1-ethylbutyl-1-en, 2-ethylbutyl-1-en, 1,1,2-trimethylpropyl-1-en, 1,2,2-trimethylpropyl-1-en, 1-ethyl-1-methylpropyl-1-en, or 1-ethyl-2-methylpropyl-1-en, and

[0242] X and Y are independently O (oxygen) or S (sulfur)

[0243] and

[0244] Q is one of the following moiety Q-1 to Q-54, Q-56 to Q-57, Q-60 to Q-89, Q-91 to Q-129, Q-131 to Q-139, Q-141 to Q-144, Q-146 to Q-180, Q-182 to Q-185, Q-193 to Q-195, Q-200 to Q-208, Q-210 to Q-370, Q-395 to Q-440:

[0245]

[0246]

[0247]

[0248]

[0249]

[0250]

[0251]

[0252]

[0253]

[0254]

[0255]

[0256]

[0257] Examples of these herbicidally active compounds within the range of chemical formula (I) include the following:

[0258] (a)

[0259] Pyridine-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate;

[0260] (b)

[0261] 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate;

[0262] (c)

[0263] 2-methoxyethyl[(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate; and

[0264] (d)

[0265] Cyanomethyl[(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate.

[0266] 2) A herbicidally active compound of general formula (II) or an agrochemically acceptable salt thereof

[0267]

[0268] (during the meal,

[0269] W represents W-1 to W-3.

[0270]

[0271] R 1 It represents hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1-yloxy, and

[0272] R 2 represents fluorine, chlorine, bromine, cyano, nitro, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethinyl, propin-1-yl, 1-butyne-1-yl, pentyne-1-yl, or hexine-1-yl, and

[0273] R 3 and R 4 are independently hydrogen, (C1-C8)-alkyl, R 13 Representing O-(C1-C8)-alkyl, (C3-C8)-cycloalkyl, (C2-C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, or heterocyclyl-(C1-C8)-alkyl,

[0274] or

[0275] R 3 and R 4 They form fully saturated or partially saturated 3- to 10-membered carbocyclic rings having optional additional substitutions with the carbon atoms to which they are bonded, and

[0276] R 5 is hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, R 13 Representing O-(C1-C8)-alkyl, (C2-C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclyl,

[0277] R 6 represents hydrogen, fluorine, chlorine, bromine, trifluoromethyl, defluoromethyl, methoxy, ethoxy, prop-1-yloxy, or but-1-yloxy, and

[0278] R 7 It represents hydrogen or methyl, and

[0279] Q represents a hydroxyl group or group Q-1, Q-2

[0280]

[0281] R 8 Silver hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R 13 , C(O)OR 13, or (C1-C8)-alkoxy-(C1-C8)-alkyl, representing,

[0282] R 9 represents hydrogen or (C1-C8)-alkyl, and

[0283] R 10 Silver, hydrogen, halogen, cyano, nitro, (C1C8)-alkyl, (C1C8)-haloalkyl, (C3C8)-cycloalkyl, (C3C8)-cycloalkyl-(C1C8)-alkyl, (C3C8)-halocycloalkyl, (C3C8)-halocycloalkyl-(C1C8)-alkyl, (C2C8)-alkenyl, (C2C8)-alkynyl, aryl, aryl-(C1C8)-alkyl, heteroaryl, heteroaryl-(C1C8)-alkyl, heterocyclil, heterocyclil-(C1C8)-alkyl, R 11 R 12 N-(C1C8)-alkyl, R 13 O-(C1C8)-alkyl, cyano-(C1C8)-alkyl, (C1C8)-alkylcarbonyloxy-(C1C8)-alkyl, (C3C8)-cycloalkylcarbonyloxy-(C1C8)-alkyl, arylcarbonyloxy-(C1C8)-alkyl, heteroarylcarbonyloxy-(C1C8)-alkyl, heterocyclylcarbonyloxy-(C1C8)-alkyl, OR 13 , NR 11 R 12 , SR 14 , S(O)R 14 , SO2R 14 , R 14 S-(C1C8)-alkyl, R 14 (O)S-(C1C8)-alkyl, R 14O2S-(C1C8)-alkyl, tris-[(C1C8)-alkyl]silyl-(C1C8)-alkyl, bis-[(C1C8)-alkyl](aryl)silyl(C1C8)-alkyl, [(C1C8)-alkyl]-bis-(aryl)silyl-(C1C8)-alkyl, tris-[(C1C8)-alkyl]silyl, bis-hydroxyboryl-(C1C8)-alkyl, bis-[(C1C8)-alkoxy]boryl-(C1C8)-alkyl, tetramethyl-1,3,2-dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1C8)-alkyl, nitro-(C1C8)-alkyl, C(O)OR 13 , C(O)R 13 , C(O)NR 11 R 12 , R 13 O(O)C-(C1C8)-alkyl, R 11 R 12 Representing N(O)C-(C1C8)-alkyl, or bis-(C1C8)-alkoxy-(C1C8)-alkyl,

[0284] R 8 and R 10 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0285] R 11 and R 12 are independently hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, C(O)R 13 , SO2R 14 , representing heterocyclil, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)-alkoxycarbonyl, (C2-C8)-alkenyloxycarbonyl, (C2-C8)-alkenyloxycarbonyl, or heterocyclil-(C1-C8)-alkyl,

[0286] R 11 and R 12 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0287] R 13 Silver hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl-amino-(C2-C6)-alkyl, aryl-(C1-C8)-alkyl-amino-(C2-C6)-alkyl, R 14 S-(C1-C8)-alkyl, R 14 (O)S-(C1-C8)-alkyl, R 14 O2S-(C1-C8)-alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, Representing heterocyclylcarbonyloxy-(C1-C8)-alkyl, aryloxy-(C1-C8)-alkyl, heteroaryloxy-(C1-C8)-alkyl, or (C1-C8)-alkoxycarbonyl,

[0288] R 14 is hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10 )-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 Representing )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino, (C1-C8)-alkylamino, aryl-(C1-C8)-amino, aryl-(C1-C6)-alkylamino, aryl-[(C1-C8)-alkyl]amino; (C3-C8)-cycloalkylamino, (C3-C8)-cycloalkyl-[(C1-C8)-alkyl]amino, N-azethidinyl, N-pyrrolidinyl, N-piperidinyl, or N-morpholinyl,

[0289] R 15 and R 16 They independently represent (C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclil.

[0290] Examples of such herbicidally active compounds within the range of chemical formula (II) are methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate(methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} propanoate or also known as flufenoxymacil), methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} propanoate, methyl 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} butanoate, methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} butanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butyric acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, ethyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, methyl (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, and (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid.

[0291] 3) A herbicidally active compound of general formula (III) or an agrochemically acceptable salt thereof

[0292]

[0293] Here:

[0294] W represents W-1 to W-3.

[0295]

[0296] R 1 It represents hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1-yloxy, and

[0297] R 2represents fluorine, chlorine, bromine, cyano, nitro, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethinyl, propin-1-yl, 1-butyne-1-yl, pentyne-1-yl, or hexine-1-yl, and

[0298] R 3 and R 4 represents hydrogen, (C1-C8)-alkyl independently of each other, and

[0299] R 5 is hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, R 13 Representing O-(C1-C8)-alkyl, (C2-C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, or heterocyclyl-(C1-C8)-alkyl,

[0300] R 3 and R 5 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0301] R 6 represents hydrogen, fluorine, chlorine, bromine, trifluoromethyl, defluoromethyl, methoxy, ethoxy, prop-1-yloxy, or but-1-yloxy, and

[0302] R 7 It represents hydrogen, methyl, and

[0303] Q represents a hydroxyl group or group Q-1, Q-2

[0304]

[0305] R 8 Silver hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R 13 , C(O)OR 13, or (C1-C8)-alkoxy-(C1-C8)-alkyl, representing,

[0306] R 9 represents hydrogen, (C1-C8)-alkyl, and

[0307] R 10 Silver, hydrogen, halogen, cyano, nitro, (C1-C8)-alkyl, (C1-C8)-haloalkyl, (C3-C8)-cycloalkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C3-C8)-halocycloalkyl, (C3-C8)-halocycloalkyl-(C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclil, heterocyclil-(C1-C8)-alkyl, R 11 R 12 N-(C1-C8)-alkyl, R 13 O-(C1-C8)-alkyl, cyano-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, heterocyclylcarbonyloxy-(C1-C8)-alkyl, OR 13 , NR 11 R 12 , SR 14 , S(O)R 14 , SO2R 14 , R 14 S-(C1-C8)-alkyl, R 14 (O)S-(C1-C8)-alkyl, R 14O2S-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl, bis-hydroxyboryl-(C1-C8)-alkyl, bis-[(C1-C8)-alkoxy]boryl-(C1-C8)-alkyl, tetramethyl-1,3,2-dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1-C8)-alkyl, nitro-(C1-C8)-alkyl, C(O)OR 13 , C(O)R 13 , C(O)NR 11 R 12 , R 13 O(O)C-(C1-C8)-alkyl, R 11 R 12 Representing N(O)C-(C1-C8)-alkyl, or bis-(C1-C8)-alkoxy-(C1-C8)-alkyl,

[0308] R 8 and R 10 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0309] R 11 and R 12 are independently hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, C(O)R 13 , SO2R 14 , representing heterocyclil, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)-alkoxycarbonyl, (C2-C8)-alkenyloxycarbonyl, (C2-C8)-alkenyloxycarbonyl, or heterocyclil-(C1-C8)-alkyl,

[0310] R 11 and R 12 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0311] R 13 Silver hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl-amino-(C2-C6)-alkyl, aryl-(C1-C8)-alkyl-amino-(C2-C6)-alkyl, R 14 S-(C1-C8)-alkyl, R 14 (O)S-(C1-C8)-alkyl, R 14 O2S-(C1-C8)-alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, Representing heterocyclylcarbonyloxy-(C1-C8)-alkyl, aryloxy-(C1-C8)-alkyl, heteroaryloxy-(C1-C8)-alkyl, or (C1-C8)-alkoxycarbonyl,

[0312] R 14 is hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10 )-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 Representing )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino, (C1-C8)-alkylamino, aryl-(C1-C8)-amino, aryl-(C1-C6)-alkylamino, aryl-[(C1-C8)-alkyl]amino; (C3-C8)-cycloalkylamino, (C3-C8)-cycloalkyl-[(C1-C8)-alkyl]amino, N-azethidinyl, N-pyrrolidinyl, N-piperidinyl, or N-morpholinyl,

[0313] R 15 and R 16 They independently represent (C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclil.

[0314] Examples of herbicidally active compounds within the range of chemical formula (III) are ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, ethyl (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-propyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-ethyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazole-3-yl)phenyl]-1-methyl-6-(trifluoromethyl)pyrimidine-2,4(1H,3H)-dione, ethyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazole-3-yl)phenyl]-1,5-dimethyl-6-sulfanylidene-1,3,5-triazinan e-2,4-dione, ethyl 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, and methyl It contains 3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate.

[0315] 4) A herbicidally active compound corresponding to a compound selected from the group consisting of A1, A2 and A3, or an agrochemically acceptable salt thereof, where:

[0316] A1 corresponds to the following:

[0317]

[0318] 2-ethoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate;

[0319] A2 corresponds to the following:

[0320]

[0321] {[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}acetic acid; and

[0322] A3 corresponds to the following:

[0323]

[0324] 2-methoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate.

[0325] 5) A herbicidally active compound of general formula (IV) or an agrochemically acceptable salt thereof

[0326]

[0327] Here

[0328] R 1 Silver, hydrogen, fluorine, chlorine, bromine, iodo, cyano, methyl, ethyl, prop-1-yl, 1-methylethyl, but-1-yl, 1-methylpropyl, 2-methylpropyl, 1,1-dimethylethyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-di-methylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl, 1-ethyl-2-methylpropyl, trifluoromethyl, difluoromethyl, pentafluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, 1,1-dimethyleth-1-yloxy, difluoromethoxy, trifluoromethoxy, pentafluoroethoxy, 2,2-difluoroethoxy, or 2,2,2-trifluoroethoxy, and

[0329] R 2 is hydrogen, fluorine, chlorine, bromine, methyl, trifluoromethyl, methoxy, ethoxy, prop-1-yloxy, or but-1-yloxy, and

[0330] R 3It is hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1-yloxy, and

[0331] R 4 is fluorine, chlorine, bromine, cyano, NO2, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethinyl, propin-1-yl, 1-butyne-1-yl, pentyne-1-yl, or

[0332] Hexine-1-yl,

[0333] R 5 , R 6 and R 7 are independently hydrogen, fluorine, chlorine, bromine, iodo, cyano, methyl, ethyl, prop-1-yl, 1-methylethyl, but-1-yl, 1-methylpropyl, 2-methylpropyl, 1,1-dimethylethyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-di-methylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl, 1-ethyl-2-methylpropyl, trifluoromethyl, difluoromethyl, pentafluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, 1,1-dimethyleth-1-yloxy, difluoromethoxy, trifluoromethoxy, pentafluoroethoxy, 2,2-difluoroethoxy, or 2,2,2-trifluoroethoxy, and

[0334] G is methylene, (methyl)methylene, (ethyl)methylene, (prop-1-yl)methylene, (prop-2-yl)methylene, (but-1-yl)methylene, (but-2-yl)methylene, (pent-1-yl)methylene, (pent-2-yl)methylene, (pent-3-yl)methylene, (dimethyl)methylene, (diethyl)methylene, ethylene, n-propylene, (1-methyl)ethyl-1-en, (2-methyl)ethyl-1-en, n-butylene, 1-methylpropyl-1-en, 2-methylpropyl-1-en, 3-methylpropyl-1-en, 1,1-dimethylethyl-1-en, 2,2-dimethylethyl-1-en, 1-ethylethyl-1-en, 2-ethylethyl-1-en, 1-(prop-1-yl)ethyl-1-en, 2-(prop-1-yl)ethyl-1-en, 1-(prop-2-yl)ethyl-1-en, 2-(prop-2-yl)ethyl-1-en, 1,1,2-trimethylethyl-1-en, 1,2,2-trimethylethyl-1-en, 1,1,2,2-tetramethylethyl-1-en, n-pentylene, 1-methylbutyl-1-en, 2-methylbutyl-1-en, 3-methylbutyl-1-en, 4-methylbutyl-1-en, 1,1-dimethylpropyl-1-en, 2,2-dimethylpropyl-1-en, 3,3-dimethylpropyl-1-en, 1,2-dimethylpropyl-1-en, 1,3-dimethylpropyl-1-en, 1-ethylpropyl-1-en, n-hexylene, 1-methylpentyl-1-en, 2-methylpentyl-1-en, 3-methylpentyl-1-en, 4-methylpentyl-1-en, 1,1-dimethylbutyl-1-en, 1,2-dimethylbutyl-1-en, 1,3-di-methylbutyl-1-en, 2,2-dimethylbutyl-1-en, 2,3-dimethylbutyl-1-en, 3,3-dimethylbutyl-1-en, 1-ethylbutyl-1-en, 2-ethylbutyl-1-en, 1,1,2-trimethylpropyl-1-en, 1,2,2-trimethylpropyl-1-en, 1-ethyl-1-methylpropyl-1-en, or 1-ethyl-2-methylpropyl-1-en, and

[0335] X and Y are independently O (oxygen) or S (sulfur)

[0336] and

[0337] Q is one of groups Q-1 to Q-25, where the arrows in the chemical formulas in the table below indicate the bonding of each group Q to the carbonyl group in general chemical formula (I):

[0338]

[0339] An example of a herbicidally active compound within the range of chemical formula (IV) is cyclopropylmethyl-(2-{2-chlor-4-fluor-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate having the following structure:

[0340]

[0341] 6) A herbicidally active compound of general formula (V) or an agrochemically acceptable salt thereof

[0342]

[0343] Here:

[0344] W represents W-1 to W-2.

[0345]

[0346] A represents nitrogen or CH;

[0347] R 1 It represents hydrogen or methyl, and

[0348] R 2 represents hydrogen or fluorine;

[0349] R 3 represents hydrogen, halogen, or (C1-C8)-alkoxy;

[0350] R 4 represents a halogen, cyano, NO2, C(O)NH2, C(S)NH2, (C1-C8)-haloalkyl, or (C2-C8)-alkynyl;

[0351] R 5 , R 6 and R 7represents hydrogen, halogen, cyano, (C1-C8)-alkyl, (C1-C8)-haloalkyl, (C1-C8)-alkoxy, or (C1-C8)-haloalkoxy independently of each other;

[0352] G represents a branched or unbranched alkylene group optionally substituted with a (C1-C3)-alkoxy;

[0353] Q represents a hydroxyl group or group Q-1, Q-2;

[0354]

[0355] R 8 Silver hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R 13 , C(O)OR 13 , or (C1-C8)-alkoxy-(C1-C8)-alkyl, representing,

[0356] R 9 represents hydrogen or (C1-C8)-alkyl, and

[0357] R 10 Silver hydrogen, halogen, cyano, NO2, (C1-C8)-alkyl, (C1-C8)-haloalkyl, (C3-C8)-cycloalkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C3-C8)-halocycloalkyl, (C3-C8)-halocycloalkyl-(C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclil, heterocyclil-(C1-C8)-alkyl, R 11 R 12 N-(C 1 -C8)-alkyl, R 13 O-(C 1-C8)-alkyl, cyano-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, heterocyclylcarbonyloxy-(C1-C8)-alkyl, OR 13 , NR 11 R 12 , SR 14 , S(O)R 14 , SO 2 R 14 , R 14 S-(C 1 -C8)-alkyl, R 14 (O)S-(C 1 -C8)-alkyl, R 14 O 2 S-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl, bis-hydroxyboryl-(C1-C8)-alkyl, bis-[(C1-C8)-alkoxy]boryl-(C1-C8)-alkyl, tetramethyl-1,3,2-dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1-C8)-alkyl, nitro-(C1-C8)-alkyl, C(O)OR 13 , C(O)R 13 , C(O)NR 11 R 12 , R 13 O(O)C-(C 1 -C8)-alkyl, R 11 R 12 N(O)C-(C 1 Representing -C8)-alkyl, or bis-(C1-C8)-alkoxy-(C1-C8)-alkyl,

[0358] R 8 and R 10They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are attached, and

[0359] R 11 and R 12 is identical or different and independently hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10 )-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, COR 13 , SO2R 14 , representing heterocyclil, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)-alkoxycarbonyl, (C2-C8)-alkenyloxycarbonyl, (C2-C8)-alkenyloxycarbonyl, or heterocyclil-(C1-C8)-alkyl,

[0360] R 11 and R 12They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are attached, and

[0361] R 13 Silver hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10 )-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl-amino-(C2-C6)-alkyl, aryl-(C1-C8)-alkyl-amino-(C2-C6)-alkyl, R 14 S-(C1-C8)-alkyl, R 14 (O)S-(C1-C8)-alkyl, R 14O2S-(C1-C8)-alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, Representing heterocyclylcarbonyloxy-(C1-C8)-alkyl, aryloxy-(C1-C8)-alkyl, heteroaryloxy-(C1-C8)-alkyl, or (C1-C8)-alkoxycarbonyl,

[0362] R 14 is hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10 )-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 Representing )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino, (C1-C8)-alkylamino, aryl-(C1-C8)-amino, aryl-(C1-C6)-alkylamino, aryl-[(C1-C8)-alkyl]amino; (C3-C8)-cycloalkylamino, (C3-C8)-cycloalkyl-[(C1-C8)-alkyl]amino, N-azethidinyl, N-pyrrolidinyl, N-piperidinyl, or N-morpholinyl,

[0363] R 15 and R 16 They independently represent (C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclil groups, or

[0364] R 15 and R 16 They form unsubstituted (C3-C7)-cycloalkyl groups together with the carbon atoms to which they are attached;

[0365] X and Y independently represent oxygen or sulfur;

[0366] Z represents nitrogen or CH.

[0367] Examples of herbicidal active compounds within the range of chemical formula (V) are ethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetic acid; Ethyl (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}phenoxy)acetate; (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}acetic acid; ethyl (2-{2-chloro-4-fluoro-5-[4-(1-fluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate; 2-methoxyethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; tetrahydrofuran-2-ylmethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; cyanomethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; methyl (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}phenoxy)(methoxy) acetate; methyl (2-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}phenoxy)(methoxy)acetate;[(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetic acid; ethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; 2-methoxyethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; tetrahydrofuran-2-ylmethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidine-1(2H)-yl]-4-fluorophenoxy}pyridine-2-yl)oxy]acetate; and includes ethyl 2-[[3-[5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-pyrimidine-1-yl]-4-fluoro-2-nitro-phenoxy]-2-pyridyl]oxy]acetate.;

[0368] 7) A herbicidally active compound corresponding to a compound selected from the group consisting of B1, B2, B3, B4, B5, and B6, or an agrochemically acceptable salt thereof, where:

[0369] B1 corresponds to the following:

[0370]

[0371] 1-ethoxy-1-oxopropane-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate;

[0372] B2 corresponds to the following:

[0373]

[0374] 2-{[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid;

[0375] B3 corresponds to the following:

[0376]

[0377] 1-methoxy-1-oxopropane-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate;

[0378] B4 corresponds to the following:

[0379]

[0380] 1-ethoxy-2-methyl-1-oxopropane-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate;

[0381] B5 corresponds to the following:

[0382]

[0383] 1-ethoxy-1-oxobutane-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate;

[0384] B6 corresponds to the following:

[0385]

[0386] 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate.

[0387] 8) A herbicidally active compound corresponding to a compound selected from the group consisting of C1, C2, C3, C4, C5, C6, C7, C8, C9, C10, C11, and C12, or an agrochemically acceptable salt thereof, where:

[0388] C1 corresponds to the following:

[0389]

[0390] 2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0391] C2 corresponds to the following:

[0392]

[0393] [({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid

[0394] C3 corresponds to the following:

[0395]

[0396] 1-ethoxy-1-oxopropane-2-yl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0397] C4 corresponds to the following:

[0398]

[0399] 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid

[0400] C5 corresponds to the following:

[0401]

[0402] Allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0403] C6 corresponds to the following:

[0404]

[0405] 1-ethoxy-2-methyl-1-oxopropane-2-yl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0406] C7 corresponds to the following:

[0407]

[0408] 2-Methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0409] C8 corresponds to the following:

[0410]

[0411] 2-(dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0412] C9 corresponds to the following:

[0413]

[0414] 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid

[0415] C10 corresponds to the following:

[0416]

[0417] Methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate

[0418] C11 corresponds to the following:

[0419]

[0420] 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide

[0421] C12 corresponds to the following:

[0422]

[0423] Ethyl 1-({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate.

[0424] 9) A herbicidally active compound of general formula (VI) or an agronomically acceptable salt thereof

[0425]

[0426] During the meal,

[0427] A is N (nitrogen) or CR 5 Representing energy,

[0428] G 1 and G 2 are independently O (oxygen) or S (sulfur), and

[0429] R 1 Silver represents fluorine, hydrogen, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1-yloxy, and

[0430] R 2represents chlorine, fluorine, bromine, iodine, cyano, nitro, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethinyl, propin-1-yl, 1-butyne-1-yl, pentyne-1-yl, or hexine-1-yl, and

[0431] R 3 and R 4 are independently hydrogen, (C1-C8)-alkyl, R 13 Representing O-(C1-C8)-alkyl, (C3-C8)-cycloalkyl, (C2-C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, or heterocyclyl-(C1-C8)-alkyl,

[0432] R 3 and R 4 They form fully saturated or partially saturated 3- to 10-membered carbocyclic rings having optional additional substitutions with the carbon atoms to which they are bonded, and

[0433] R 5 is hydrogen, halogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, or OR 13 Represents,

[0434] R 6 Silver represents fluorine, hydrogen, chlorine, bromine, trifluoromethyl, defluoromethyl, methoxy, ethoxy, prop-1-yloxy, but-1-yloxy, or methyl,

[0435] R 7 It represents hydrogen or methyl, and

[0436] Q represents a hydroxyl group or group Q-1, Q-2

[0437]

[0438] R 8 Silver hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R 13 , C(O)OR 13, or (C1-C8)-alkoxy-(C1-C8)-alkyl, representing,

[0439] R 9 represents hydrogen or (C1-C8)-alkyl, and

[0440] R 10 Silver, hydrogen, halogen, cyano, nitro, (C1C8)-alkyl, (C1C8)-haloalkyl, (C3C8)-cycloalkyl, (C3C8)-cycloalkyl-(C1C8)-alkyl, (C3C8)-halocycloalkyl, (C3C8)-halocycloalkyl-(C1C8)-alkyl, (C2C8)-alkenyl, (C2C8)-alkynyl, aryl, aryl-(C1C8)-alkyl, heteroaryl, heteroaryl-(C1C8)-alkyl, heterocyclil, heterocyclil-(C1C8)-alkyl, R 11 R 12 N-(C1C8)-alkyl, R 13 O-(C1C8)-alkyl, cyano-(C1C8)-alkyl, (C1C8)-alkylcarbonyloxy-(C1C8)-alkyl, (C3C8)-cycloalkylcarbonyloxy-(C1C8)-alkyl, arylcarbonyloxy-(C1C8)-alkyl, heteroarylcarbonyloxy-(C1C8)-alkyl, heterocyclylcarbonyloxy-(C1C8)-alkyl, OR 13 , NR 11 R 12 , SR 14 , S(O)R 14 , SO2R 14 , R 14 S-(C1C8)-alkyl, R 14 (O)S-(C1C8)-alkyl, R 14O2S-(C1C8)-alkyl, tris-[(C1C8)-alkyl]silyl-(C1C8)-alkyl, bis-[(C1C8)-alkyl](aryl)silyl(C1C8)-alkyl, [(C1C8)-alkyl]-bis-(aryl)silyl-(C1C8)-alkyl, tris-[(C1C8)-alkyl]silyl, bis-hydroxyboryl-(C1C8)-alkyl, bis-[(C1C8)-alkoxy]boryl-(C1C8)-alkyl, tetramethyl-1,3,2-dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1C8)-alkyl, nitro-(C1C8)-alkyl, C(O)OR 13 , C(O)R 13 , C(O)NR 11 R 12 , R 13 O(O)C-(C1C8)-alkyl, R 11 R 12 Representing N(O)C-(C1C8)-alkyl, or bis-(C1C8)-alkoxy-(C1C8)-alkyl,

[0441] R 8 and R 10 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0442] R 11 and R 12 are independently hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, C(O)R 13 , SO2R 14 , representing heterocyclil, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)-alkoxycarbonyl, (C2-C8)-alkenyloxycarbonyl, (C2-C8)-alkenyloxycarbonyl, or heterocyclil-(C1-C8)-alkyl,

[0443] R 11 and R 12 They form fully saturated or partially saturated 3- to 10-membered monocyclic or acyclic rings with optionally interposed heteroatoms and optionally additional substitutions together with the carbon atoms to which they are bonded, and

[0444] R 13 Silver hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl-amino-(C2-C6)-alkyl, aryl-(C1-C8)-alkyl-amino-(C2-C6)-alkyl, R 14 S-(C1-C8)-alkyl, R 14 (O)S-(C1-C8)-alkyl, R 14 O2S-(C1-C8)-alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, Representing heterocyclylcarbonyloxy-(C1-C8)-alkyl, aryloxy-(C1-C8)-alkyl, heteroaryloxy-(C1-C8)-alkyl, or (C1-C8)-alkoxycarbonyl,

[0445] R 14 is hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C 10 )-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkinyl, (C3-C 10 )-cycloalkyl, (C3-C 10 )-halocycloalkyl, (C4-C 10 )-cycloalkenyl, ( C4-C 10 )-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C 10 Representing )-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino, (C1-C8)-alkylamino, aryl-(C1-C8)-amino, aryl-(C1-C6)-alkylamino, aryl-[(C1-C8)-alkyl]amino; (C3-C8)-cycloalkylamino, (C3-C8)-cycloalkyl-[(C1-C8)-alkyl]amino, N-azethidinyl, N-pyrrolidinyl, N-piperidinyl, or N-morpholinyl,

[0446] R 15 and R 16 They independently represent (C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclil.

[0447] Examples of herbicidally active compounds within the range of chemical formula (VI) include the following:

[0448]

[0449]

[0450]

[0451]

[0452] Combinations of PPO herbicides are included within the scope of the present disclosure. For example, the PPO expression cassette of soybean event Gm_CSM63717 provides resistance to a combination of flumioxazine and piraflufen-ethyl.

[0453] As used herein, synthetic auxins include, but are not limited to, benzoate auxins or herbicides, phenoxy acid herbicides, aryl picolinate herbicides, and pyridinyl oxy acid herbicides. Examples of benzoate herbicides include, but are not limited to, dicamba (3,6-dichloro-2-methoxybenzoic acid), dicamba salts, dicamba-butothyl, dicamba-diglycolamine salt, dicamba-dimethylammonium, dicamba-diethanolammonium, dicamba-isopropylammonium, dicamba-potassium, dicamba-sodium, and dicamba-trolamine. Examples of phenoxy acid herbicides include 2,4-D(2,4-dichlorophenoxyacetic acid) , 2,4-D-Butotyl, 2,4-D-Butyl, 2,4-D-Choline, 2,4-D-Dimethylammonium, 2,4-D-Diolamine, 2,4-D-Ethyl, 2,4-D-2-Ethylhexyl, 2,4-D-Isobutyl, 2,4-D-Isoctyl, 2,4-D-Isopropyl, 2,4-D-Isopropylammonium, 2,4-D-Potassium, 2,4-D-Sodium, 2,4-D-Triisopropanolammonium, 2,4-D-Trolamine, Clomeprop, Dichlorprop, Fenoprop, MCPA(2-Methyl-4-Chlorophenoacetic Acid), MCPA-Butotyl, MCPA-Dimethylammonium, MCPA-2-Ethylhexyl, MCPA-Isopropylammonium, MCPA-Potassium, MCPA-sodium, MCPA-thioethyl, 2,4-DB, MCPB (4-(4-chloro-2-methylphenoxy)butanoic acid), MCPB-methyl, MCPB-ethyl-sodium, and mecoprop are included, but not limited thereto. Examples of aryl picolinate herbicides include, but are not limited thereto, halauxifen, halauxifen-methyl, and florpyroxifen-benzyl. Examples of pyridinyloxyacid herbicides include, but are not limited to, triclopyr, fluroxipyr, aminopyralide, and picloram.

[0454] As used herein, inhibitors of glutamine synthase include, but are not limited to, phosphinothricin, glufosinate, glufosinate salts, glufosinate-ammonium, glufosinate-sodium, glufosinate-P, L-glufosinate-ammonium, L-glufosinate-sodium, and any combination thereof.

[0455] As used herein, β-triketone HPPD inhibitors may be selected from the group consisting of mesotrione, benzobicyclon (BBC), tembotrion, sulfotrione, tefuryltrione, and combinations of any of these.

[0456] As used herein, inhibitors of 5-enolpyrubilchimate-3-phosphate synthase (EPSPS) include, but are not limited to, glyphosate, glyphosate salts, glyphosate-isopropylammonium, glyphosate-ammonium, glyphosate-dimethylammonium, glyphosate-trimethium (= sulfosate), glyphosate-diammonium, glyphosate-potassium, and glyphosate-sodium and any combination thereof.

[0457] As used herein, “herbicide resistance” or “herbicide resistance” or “resistance” means the ability to resist the toxic effects of a herbicide when applied, for example, that is not affected wholly or partially by the presence or application of one or more herbicide(s). A cell, seed, or plant has “herbicide resistance” or “improved resistance” if it can maintain at least some normal growth or phenotype in the presence of one or more herbicide(s). A trait is a herbicide resistance trait if its presence can confer improved herbicide resistance to a cell, plant, or seed compared to wild-type or control cells, plants, or seeds. Crops containing herbicide resistance traits can continue to grow in the presence of herbicides and can be minimally affected by the presence of herbicides. A protein confers “herbicide resistance” if the expression of the protein can confer improved herbicide resistance to a cell, plant, or seed compared to wild-type or control cells, plants, or seeds. Examples of herbicide resistance proteins are protoporpyrinogen oxidase, dicamba monooxygenase, phosphinothricin N-acetyltransferase, alpha-ketoglutarate-dependent non-heme iron deoxygenase, triketone dioxygenase, and 5-etholpyrubilchimate-3-phosphate synthase. Herbicide resistance can be complete or partial insensitivity to a specific herbicide and can be expressed as percentage (%) resistance or insensitivity to a specific herbicide.

[0458] As used herein, “herbicide damage” or “damage” refers to damage to a plant resulting from the application of one or more herbicides. “Damage rate” or “damage percentage” refers to the percentage of plant leaf area showing damage caused by the application of herbicides, such as necrosis (brown or dead tissue), chlorosis (yellow tissue or yellow spots), and malformation (malformed leaves or plant structures, epinasty or twisting of stems, leaf indentation), based on visual assessment. It is measured on a scale from 0 to 100, where “0” indicates no crop damage and “100” indicates complete crop damage (death).

[0459] For soybean plants containing or including soybean event Gm_CSM63717, the plants will have reduced damage after the application of one or more PPO inhibitors. For example, soybean plants containing or including soybean event Gm_CSM63717, compared to identical soybean plants that do not contain soybean event Gm_CSM63717, flumioxazine, epirifenacil, lactophen, asifluorophene, piraflufen, piraflufen-ethyl, oxadiazone, butafenacil, 피리딘-2-일메틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 2-메톡시에틸 [(3-{2-시아노-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 시아노메틸 [(3-{2-브로모-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}피리딘-2-일)옥시]아세테이트, 사이클로프로필메틸 (2-{2-클로로-4-플루오로-5-[3-메틸-2,6-디옥소-4-(트리플루오로메틸)-3,6-디하이드로피리미딘-1(2H)-일]페녹시}페녹시)아세테이트, After application of a PPO herbicide such as methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoxymasil), fomesafen, safluphenacil, sulfentrazone, tiafenacil, and trifludimoxazine, or any combination thereof, there will be less than 5% damage, less than 10% damage, less than 15% damage, or less than 20% damage.

[0460] As used herein, "weed" refers to any unwanted plant. Plants that may generally be considered unwanted for agricultural or horticultural purposes (e.g., amaranthus ( Amaranthus) Species) may be considered unwanted in certain situations (e.g., a single species of crop plant in a field of different species, also known as a native plant). Weeds are commonly known in the art and vary by geographical region, season, growing environment, and time. Lists of weed species are available from agricultural and scientific societies and activities (e.g., American Society of Weed Science, Canadian Weed Science Society, Brazilian Weed Science Society, International Weed Science Society, and the International Survey of Herbicide-Resistant Weeds), government agencies (e.g., U.S. Department of Agriculture and the Australian Department of Environment and Energy), and industry and farmers' associations. A major weed problem in soybean production is the water gun ( Amaranthus tuberculatus ), giant ragweed( Ambrosia tripida Ragweed like ) Ambrosia species ), and common ragweed ( Ambrosia artemisifolia ), common goosefoot ( Chenopodium album ), morning glory species( Ipomoea species ), horsehair ( Coniza canadensis ), Marestale( Erigaron Canadensis ), Palmer Amaranth( Amaranthus armadil ), pigweed( Amaranthus species ), Velvetleaf( Abutilon teophrasti Medique), general coclebourg ( Xantium strumarium ), Foxtail( Cetaria species ), crab pudding ( Digitalia species ), Bunnyyard Pool( Echinocloa Crusgali ), Jonsongras( Sorgum Halepens ), and thistle( Sircium species Includes )(Heap 2021; Shoup et al , 2016).

[0461] A method for controlling or preventing weed growth in a region is provided. One example of such a method comprises the step of controlling weeds in a region without damage to the soybeans or with less than about 10% damage to the soybeans by applying an effective amount of a soybean and PPO herbicide containing event Gm_CSM63717 in the region. The method comprises applying one or more PPO herbicides, wherein soybean seeds or plants containing event Gm_CSM63717 are sown in the region before, at, or after the application of the herbicide, and the application of the herbicide prevents or inhibits weed growth and does not damage the soybean plants containing event Gm_CSM63717 or with less than about 5-20% damage. The plant growing region may or may not contain weed seeds or plants at the time of herbicide application. The herbicide(s) used in the method described herein may be applied alone, sequentially, or in combination with one or more herbicide(s) during the growing season. The herbicide(s) used in the method described herein may be applied temporally (e.g., as a tank mixture or sequential application), spatially (e.g., at different points during the growing season, including before and after sowing soybean seeds), or both, in combination with one or more herbicide(s). For example, a method for controlling weeds is provided, comprising the steps of sowing seeds containing soybean event Gm_CSM63717 in a region without damage to plants containing soybean event Gm_CSM63717 or with damage of about 5-20% or less, for the purpose of controlling weeds in a region, and applying a herbicidally effective amount of one or more PPO herbicides alone or in any combination with another herbicide over the growing season.Such application of the herbicide(s) may be before sowing (any point in time before sowing seeds containing soybean event Gm_CSM63717, including for exhaustion purposes, i.e., applied to weeds that are germinating or present before sowing plants), before germination (any point in time after sowing seeds containing soybean event Gm_CSM63717 and before germination of plants containing soybean event Gm_CSM63717), or after germination (any point in time after germination of plants containing soybean event Gm_CSM63717). Multiple applications of one or more herbicides over the growing season, or herbicides applied in combination or individually, for example, two applications (e.g., application before sowing and application after germination, or application before germination and application after germination) or three or more applications (e.g., application before sowing and two applications after germination).

[0462] In carrying out the methods described herein, the application of herbicides may be at the recommended commercial rate or any fraction or multiple thereof, e.g., twice the recommended commercial rate. Depending on the herbicide and formulation, the herbicide rate may be expressed as pound of acid equivalent per acre (lb ae / acre), pound of active ingredient per acre (lb ai / acre), or pound of active ingredient per hectare (lb ai / ha). 1 gram per hectare is equal to 0.000892179 pounds per acre. The use of acres or hectares in the herbicide application rates provided herein is merely directional; herbicide application rates of equivalent dosages for any rate provided herein may be used in areas larger or smaller than acres.The herbicides applied are flumioxazine, epirifenacil, lactofen, asifluorofen, piraflufen, piraflufen-ethyl, oxadiazone, butafenacil, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyclopropylmethyl(2-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate, methyl It may include at least one PPO herbicide comprising, but not limited to, (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoxymasil), fomesafene, ciflufenacil, sulfentrazone, tiafenacil, and trifludimoxazine, and any combination thereof. The plant growth area may or may not contain weed plants at the time of herbicide application.

[0463] The effective amount of PPO herbicide can be from about 0.0009 lb / acre to about 1.5 lb / acre over the growing season. Table 3 provides examples of various PPO herbicides and application rates that can be used to control weeds in soybean crop growing areas sown with Gm_CSM63717.

[0464]

[0465]

[0466] A method for controlling or preventing weed growth in a region is provided. Examples of such a method include the steps of sowing soybeans in the region containing event Gm_CSM63717, event Gm_CSM63714 and / or event MON89788, and applying one or more herbicides selected from the group consisting of PPO herbicides, dicamba, glufosinate, 2,4-D, β-triketone HPPD inhibitors, glyphosate, and any combination thereof, which have no damage or less than 10% damage. The method comprises the step of applying one or more herbicides selected from the group consisting of PPO her...

Claims

Claim 1 A recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO. 10, SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, and SEQ ID NO. 9, a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the entire length of SEQ ID NO. 10 or the entire length of SEQ ID NO. 9, and a complete complement of any of the above. Claim 2 In claim 1, the recombinant DNA molecule is derived from a soybean plant, seed, plant part, plant cell, offspring plant, or commercial product containing soybean event Gm_CSM63717, which is a representative sample of a seed containing the event deposited under ATCC accession number PTA-127604. Claim 3 The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule comprises a soybean plant, seed, plant part, plant cell, or offspring plant, or a product produced therefrom, comprising soybean event Gm_CSM63717, which is a representative sample of a seed comprising the event deposited under ATCC accession number PTA-127604. Claim 4 In claim 1, the recombinant DNA molecule is formed by inserting a heterologous nucleic acid molecule into the genomic DNA of a soybean plant or soybean cell. Claim 5 A recombinant DNA molecule according to claim 1, wherein the recombinant DNA molecule comprises an amplicon diagnosis for the presence of soybean event Gm_CSM63717. Claim 6 A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that specifically hybridizes with the soybean event Gm_CSM63717 DNA in a sample under strict hybridization conditions, wherein detecting the hybridization of the DNA molecule under said strict hybridization conditions is a diagnosis of the presence of the soybean event Gm_CSM63717 in the sample. Claim 7 A DNA molecule comprising a polynucleotide segment of sufficient length to function as a specific DNA probe for detecting at least one of the following in a sample: a 5' junction sequence between the lateral soybean genomic DNA and the transform insert of soybean event Gm_CSM63717; a 3' junction sequence between the transform insert of soybean event Gm_CSM63717 and the lateral soybean genomic DNA; SEQ ID NO. 9; and a fragment of SEQ ID NO. 9 comprising adjacent nucleotides of SEQ ID NO. 9 of sufficient length to identify the sequence as a fragment of the transform insert of Gm_CSM63717. Claim 8 A DNA molecule according to claim 6 or 7, wherein the DNA probe comprises SEQ ID NO.

178. Claim 9 A DNA molecule according to claim 6 or 7, wherein the DNA molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, and a complement of any of the above. Claim 10 In any one of paragraphs 6 to 9, the sample is a DNA molecule derived from a soybean plant, seed, plant part, plant cell, offspring plant, or commercial product. Claim 11 A DNA molecule pair comprising a first DNA molecule and a second DNA molecule, wherein the first and second DNA molecules are different from each other and each comprises a fragment of SEQ ID NO. 10 or its complement and functions as a DNA primer when used together in an amplification reaction with DNA comprising soybean event Gm_CSM63717 to produce an amplicon diagnosis for soybean event Gm_CSM63717 in a sample. Claim 12 In claim 11, a pair of DNA molecules wherein the first and second DNA molecules comprise SEQ ID NO. 176 and SEQ ID NO.

177. Claim 13 A pair of DNA molecules according to claim 11, wherein the amplicon comprises a nucleotide sequence selected from the group consisting of: SEQ ID NO. 1; SEQ ID NO. 2; SEQ ID NO. 3; SEQ ID NO. 4; SEQ ID NO. 5; SEQ ID NO. 6; SEQ ID NO. 7; SEQ ID NO. 8; SEQ ID NO. 9; SEQ ID NO. 10; and a fragment of any of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, and SEQ ID NO. 8, wherein the fragment is at least 10 nucleotide long and comprises nucleotides 1,000-1,001 or 4,201-4,202 of SEQ ID NO.

10. Claim 14 A method for detecting the presence of soybean event Gm_CSM63717 in a sample derived from soybean seeds, plants, plant parts, plant cells, offspring plants, or commercial products, the method comprising: a) contacting the sample with a DNA molecule functioning as a DNA probe of any one of claims 6 to 10; b) applying the sample and the DNA molecule functioning as a probe to strict hybridization conditions; and c) detecting the hybridization of the DNA molecule functioning as a probe for the DNA molecule in the sample, wherein the hybridization of the DNA molecule functioning as a probe for the DNA molecule in the sample is a diagnosis of the presence of soybean event Gm_CSM63717 in the sample. Claim 15 A method for detecting the presence of soybean event Gm_CSM63717 in a sample derived from soybean seeds, plants, plant parts or plant cells, offspring plants or commercial products, the method comprising: a) contacting the sample with a DNA molecule pair of any one of claims 11 to 13; b) performing an amplification reaction sufficient to produce a DNA amplicon; and c) detecting the presence of the DNA amplicon; wherein the DNA amplicon comprises at least one of the following: a 5' junction sequence between lateral soybean genomic DNA and a transform insert of soybean event Gm_CSM63717, a 3' junction sequence between lateral soybean genomic DNA and a transform insert of soybean event Gm_CSM63717, SEQ ID NO. 9, and a fragment of SEQ ID NO. 9 comprising adjacent nucleotides of SEQ ID NO. 9 of a length sufficient to identify the sequence as a fragment of the transform insert of Gm_CSM63717; and the presence of the above DNA amplicon indicates the presence of the soybean event Gm_CSM63717 in the sample, method. Claim 16 The method of claim 15, wherein the DNA amplicon is at least 10 nucleotide lengths, at least 11 nucleotide lengths, at least 12 nucleotide lengths, at least 13 nucleotide lengths, at least 14 nucleotide lengths, at least 15 nucleotide lengths, at least 16 nucleotide lengths, at least 17 nucleotide lengths, at least 18 nucleotide lengths, at least 19 nucleotide lengths, at least 20 nucleotide lengths, at least 25 nucleotide lengths, at least 30 nucleotide lengths, at least 35 nucleotide lengths, at least 40 nucleotide lengths, at least 45 nucleotide lengths, at least 50 nucleotide lengths, at least 60 nucleotide lengths, at least 70 nucleotide lengths, at least 80 nucleotide lengths, at least 90 nucleotide lengths, or at least 100 nucleotide lengths. Claim 17 A method according to claim 15 or 16, wherein the DNA amplicon comprises: a nucleotide sequence selected from the group consisting of SEQ ID NO. 10; SEQ ID NO. 9; SEQ ID NO. 8; SEQ ID NO. 7; SEQ ID NO. 6; SEQ ID NO. 5; SEQ ID NO. 4; SEQ ID NO. 3; SEQ ID NO. 2; and a fragment of any of SEQ ID NO. 10, SEQ ID NO. 8, SEQ ID NO. 7, SEQ ID NO. 6, SEQ ID NO. 5, SEQ ID NO. 4, SEQ ID NO. 3, SEQ ID NO. 2, and SEQ ID NO. 1, which is at least 10 nucleotide lengths and comprises 1,000-1,001 or 4,201-4,202 nucleotides of SEQ ID NO.

10. Claim 18 A method for detecting the presence of the soybean event Gm_CSM63717 in a sample of DNA derived from soybean seeds, plants, plant parts, plant cells, offspring plants, or commercial products, the method comprising: a) contacting the sample with a DNA molecule functioning as a probe according to any one of claims 6 to 10; and b) performing a sequencing reaction to produce a target sequence, wherein the target sequence is a nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10; and a complete complement of any of these. A method comprising a fragment of any of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, and SEQ ID NO. 10, which is at least 10 nucleotide long and comprises nucleotides 1,000-1,001 or 4,201-4,202 of SEQ ID NO.

10. Claim 19 A method for detecting the presence of soybean event Gm_CSM63717 in a sample derived from soybean seeds, plants, plant parts, cells, offspring plants, or commercial products, the method comprising: a) contacting the sample with an antibody specific to a PPO (protoporpyrinogen oxidase) protein encoded by soybean event Gm_CSM63717; and b) detecting the binding of the antibody to the protein in the sample, wherein the binding of the antibody indicates the presence of soybean event Gm_CSM63717 in the sample. Claim 20 A DNA detection kit for detecting the presence of soybean event Gm_CSM63717 in a sample, wherein the kit comprises a) a DNA primer pair of any one of claims 11 to 13; and / or b) a DNA molecule functioning as a probe of any one of claims 6 to 10. Claim 21 A protein detection kit for detecting the presence of soybean event Gm_CSM63717 in a sample, wherein the kit comprises an antibody specific to a PPO protein encoded by soybean event Gm_CSM63717; and detecting the binding of the antibody to the protein encoded by soybean event Gm_CSM63717 in the sample is a diagnosis of the presence of soybean event Gm_CSM63717 in the sample. Claim 22 A method for determining the compatibility of a soybean plant, plant part, plant seed, or plant cell containing soybean event Gm_CSM63717, comprising: a) contacting a sample containing DNA derived from a soybean plant, plant part, plant seed, or plant cell with a first primer set capable of producing a first amplicon diagnosis for the presence of soybean event Gm_CSM63717 and a second primer set capable of producing a second amplicon diagnosis for wild-type soybean genomic DNA not containing soybean event Gm_CSM63717; b) performing a nucleic acid amplification reaction; and c) a step of detecting a first amplicon and a second amplicon, wherein the presence of the two amplicons indicates that the plant, plant part, seed, or cell is heterozygous for the soybean event Gm_CSM63717, and the presence of only the first amplicon indicates that the plant, plant part, seed, or cell is homozygous for the soybean event Gm_CSM63717, comprising a method. Claim 23 A method according to claim 22, wherein the first primer set comprises SEQ ID NO. 176 and SEQ ID NO. 177, and the second primer set comprises SEQ ID NO. 208 and SEQ ID NO.

209. Claim 24 A method for determining the compatibility of a soybean plant, plant part, plant seed, or plant cell containing soybean event Gm_CSM63717, comprising: a) contacting a sample containing DNA derived from a soybean plant, plant part, plant seed, or plant cell with a set of probes comprising at least a first probe that specifically hybridizes to soybean event Gm_CSM63717, and a second probe that specifically hybridizes to soybean genomic DNA disrupted by the insertion of heterologous DNA of soybean event Gm_CSM63717 but does not hybridize to soybean event Gm_CSM63717; and b) a step of hybridizing a set of probes with a sample under strict hybridization conditions, wherein detecting hybridization of only the first probe under said hybridization conditions is a diagnosis for a soybean plant, plant part, seed, or plant cell that is homozygous to the soybean event Gm_CSM63717, and detecting hybridization of both the first probe and the second probe under said hybridization conditions is a diagnosis for a soybean plant, plant part, seed, or plant cell that is heterozygous to the soybean event Gm_CSM63717. Claim 25 In claim 24, the method wherein the probe set comprises SEQ ID NO. 178 and SEQ ID NO.

210. Claim 26 A DNA construct comprising an expression cassette, wherein the expression cassette is in an operable link i) Medicago Trunkatula ii) the promoter, leader sequence, and intron sequence of ubiquitin 2 (UBQ), Adansonia Digitata iii) Codon-optimized chloroplast transfer peptide coding sequence of APG6 (Albino and Pale Green 6), Enterobacter cloaca iv) a DNA construct comprising a codon-optimized protoporpyrinogen oxidase coding sequence, and iv) a synthetic 3' UTR sequence. Claim 27 In claim 26, the DNA construct comprises SEQ ID NO.

9. Claim 28 A DNA construct according to claim 26 or 27, wherein the 5' or 3' end of the said construct further comprises: a) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 11 or SEQ ID NO. 14; and / or b) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 12 or SEQ ID NO.

15. Claim 29 A DNA construct comprising a polynucleotide having a sequence identical to at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% of the total length of SEQ ID NO. 9, wherein the DNA construct comprises, at the 5' or 3' end of the construct, (i) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19 of SEQ ID NO. 11 or SEQ ID NO. 14, At least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides; and / or (ii) a DNA construct comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 12 or SEQ ID NO.

15. Claim 30 A DNA construct according to any one of claims 26 to 29, wherein the construct comprises one or more nucleotide sequences selected from SEQ ID NOs 36-105 at the 5' end of the construct. Claim 31 A DNA construct according to any one of claims 26 to 30, wherein the construct comprises one or more nucleotide sequences selected from SEQ ID NOs 106-175 at the 3' end of the construct. Claim 32 A method for controlling or preventing weed growth in a region, comprising the steps of sowing soybeans containing event Gm_CSM63717 in the region and applying an effective amount of PPO herbicide to control weeds in the region without damage to the soybeans or with less than about 10% damage to the soybeans. Claim 33 A method for controlling native soybeans containing soybean event Gm_CSM63717 in a region, comprising the step of applying a herbicidally effective amount of at least one herbicide other than a PPO herbicide, wherein the application of the herbicide prevents the growth of soybeans containing soybean event Gm_CSM63717. Claim 34 In paragraph 33, the herbicide other than the PPO herbicide is selected from the group consisting of atrazine, topramezone, clopyralide, pyritiobak, fluoromethurone, (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU), triploxisulfuron, paraquat, diquat, glyphosate, glufosinate, and any combination thereof. Claim 35 A method for obtaining seeds or soybean plants resistant to PPO herbicides, comprising: a) obtaining at least one offspring seed containing soybean event Gm_CSM63717 or a population of plants grown therefrom; and b) identifying at least one first offspring seed containing soybean event Gm_CSM63717 or a plant grown therefrom. Claim 36 In claim 35, the step of identifying offspring seeds containing the soybean event Gm_CSM63717 or plants grown therefrom comprises: a) culturing offspring seeds or plants to produce offspring plants; b) treating offspring plants with an effective amount of PPO herbicide; and c) selecting offspring plants resistant to PPO herbicide. Claim 37 A method according to claim 35 or 36, wherein identifying a offspring seed containing the soybean event Gm_CSM63717 or a plant grown therefrom comprises the step of detecting the presence of the soybean event Gm_CSM63717 in a sample derived from the offspring seed or the plant grown therefrom. Claim 38 A method according to any one of claims 35 to 37, wherein identifying a offspring seed containing the soybean event Gm_CSM63717 or a plant grown therefrom comprises the step of detecting the presence of a PPO protein encoded by the soybean event Gm_CSM63717 in a sample derived from the offspring seed or the plant grown therefrom. Claim 39 A method for improving resistance to PPO herbicides in soybean plants, comprising: a) inserting a DNA construct of any one of claims 26 to 31 into the genome of a soybean cell; b) generating a soybean plant from a soybean cell; and c) selecting a soybean plant containing the DNA construct. Claim 40 A method according to claim 39, wherein the selecting step comprises treating soybean cells or plants with an effective amount of PPO herbicide. Claim 41 A soybean plant, plant seed, plant part, or plant cell comprising a polynucleotide having a sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, and SEQ ID NO. 10, a polynucleotide having a sequence identical to the entire length of SEQ ID NO. 10 or the entire length of SEQ ID NO. 9 by at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9%, and a complete complement of any of the above. Claim 42 In paragraph 41, the soybean plant, plant seed, plant part, or plant cell in which the plant, plant seed, plant part, or plant cell expresses a PPO herbicide resistance gene. Claim 43 In paragraph 41 or 42, the soybean plant, plant seed, plant part, or plant cell is resistant to one or more PPO herbicides. Claim 44 A soybean plant, plant seed, plant part, or plant cell resistant to one or more PPO herbicides, wherein said soybean plant, plant seed, plant part, or plant cell comprises a DNA construct of any one of claims 26 to 31. Claim 45 A soybean plant, plant seed, plant part, or plant cell according to any one of claims 41 to 44, wherein the plant, plant seed, plant part, or plant cell further comprises at least one additional transgenic gene for resistance to at least one additional herbicide. Claim 46 A soybean plant, plant seed, plant part, or plant cell comprising, in any one of claims 41 to 45, the plant, plant seed, plant part, or plant cell comprising the soybean event Gm_CSM63717, which is a representative sample of a seed comprising the event deposited under ATCC accession number PTA-127604. Claim 47 In any one of paragraphs 41 to 46, the soybean plant, plant seed, plant part, or plant cell is further defined as a soybean plant, plant seed, plant part, or plant cell of any generation of a soybean plant containing the soybean event Gm_CSM63717, or a soybean plant part, plant seed, or plant cell derived therefrom. Claim 48 A soybean plant, plant part, plant seed, or plant cell containing soybean event Gm_CSM63717, which is a representative sample of a seed containing soybean event Gm_CSM63717 deposited under ATCC accession number PTA-127604. Claim 49 A soybean plant part according to any one of claims 41 to 48, wherein the plant part comprises a microspore, pollen, anther, ovule, ovary, pod, flower, embryo, stem, bud, node, leaf, root, or callus. Claim 50 A soybean plant, plant seed, plant part, or plant cell, wherein, in any one of claims 41 to 49, the soybean seed, plant, plant part, or cell is obtained by the method of any one of claims 35 to 40. Claim 51 A soybean plant, plant cell, plant part, or plant seed comprising a recombinant DNA construct incorporated on chromosome 13, wherein the recombinant DNA construct confers resistance to at least one PPO herbicide, the recombinant DNA construct is incorporated at a location on the chromosome, and on its side at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides; and / or (ii) a soybean plant, plant cell, plant part or plant seed having at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 adjacent nucleotides of SEQ ID NO. 12 or SEQ ID NO.

15. Claim 52 In claim 51, a soybean plant, plant cell, plant part, or plant seed, wherein at least 50 adjacent nucleotides of SEQ ID NO. 11 or SEQ ID NO. 14 comprise one or more nucleotide sequences selected from SEQ ID NOs. 36-105. Claim 53 A soybean plant, plant cell, plant part, or plant seed according to claim 51 or 52, wherein at least 50 adjacent nucleotides of SEQ ID NO. 12 or SEQ ID NO. 15 comprise one or more nucleotide sequences selected from SEQ ID NOs. 106-175. Claim 54 A soybean plant, plant cell, plant part, or plant seed, or a method, wherein the PPO herbicide is selected from the group consisting of diphenyl ether, N-phenylphthalimide, oxadiazole, oxazolidinedione, phenylpyrazole, pyrimidinedione, thiadiazole, triazolinone, benzoxazinone derivatives, other PPO herbicides, and any combination thereof. Claim 55 In claim 54, the diphenyl ether is selected from the group consisting of acifluorophene, biphenox, ethoxyphene, fluorodiphene, fluoronitrophene, furyloxyphene, halosafen, clomethoxyphene, chlornitrophene, ethoxyphene-ethyl, fluoroglycopene, lactophene, nitrophene, oxyfluorophene, fomesafen, any salt thereof, and any ester thereof; N-phenylphthalimide is selected from the group consisting of sinidone-ethyl, flumiclorac, flumiclorac-pentyl, and flumioxazine; oxadiazole is selected from the group consisting of oxadiargyl and oxadiazone; oxazolidinedione is pentoxazone; and phenylpyrazole is selected from the group consisting of fluazolate, piraflufene, and piraflufene-ethyl; The pyrimidinedione is selected from the group consisting of benzphenidizone, butafenacil, epiripencacil (S-3100), flupropacil, flufenoxymasil, saflufenacil, and thiafenacil; the thiadiazole is selected from the group consisting of fluthiacet-methyl and thidiazimine; the triazolinone is selected from the group consisting of azafenidine, bencarbazone, carpentrazone, their salts and esters, and sulfentrazone; and the benzoxazinone derivative is 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3,4-dihydro-3-oxo-4-prop-2-inyl-2H-1,4-benzoxazine-6-yl)-1,3,5-triazinan-2,4-dione (trifludimoxazine)). Other PPO herbicides include chlorphthalim, flufenpyr, flufenpyr-ethyl, flumipropin, pyraclonil, profluazole, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}pyridine-2-yl)oxy]acetate; cyclopropylmethyl (2-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}phenoxy)acetate; Methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate(fluphenoxymacil), methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} propanoate, methyl 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy} butanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-Dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate methyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, ethyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoate, methyl (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoate, 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy}propanoic acid, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, ethyl (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-propyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-5-ethyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazole-3-yl)phenyl]-1-methyl-6-(trifluoromethyl)pyrimidine-2,4(1H,3H)-dione, ethyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,[5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanilidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazole-3-yl)phenyl]-1,5-dimethyl-6-sulfanylidene-1,3,5-triazinan-2,4-dione, ethyl 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, methyl 3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2]oxazole-6a-carboxylate, 2-ethoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,A soybean plant, plant cell, plant part, or plant seed, or a method selected from the group consisting of 6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate, {[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}acetic acid, and 2-methoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidine-1(2H)-yl]phenoxy}cyclopropanecarboxylate. Claim 56 A method according to any one of claims 32, 36 through 38, 40, 54, and 55, wherein the effective amount of the PPO herbicide is about 0.0009 lb / acre to about 1.5 lb / acre over the growing season. Claim 57 A method for producing offspring soybean plants containing soybean event Gm_CSM63717, comprising: a) sexually cross-breeding a first soybean plant containing soybean event Gm_CSM63717 with itself or a second soybean plant; b) collecting one or more seeds produced by cross-breeding; c) growing one or more seeds to produce one or more offspring plants; and d) selecting at least one first offspring plant or seed containing soybean event Gm_CSM63717. Claim 58 Inbreed or hybrid soybean plants or seeds containing soybean event Gm_CSM63717 produced by the method of paragraph 57. Claim 59 A non-living or non-renewable soybean plant material comprising a recombinant DNA molecule of any one of claims 1 to 4 or a DNA construct of any one of claims 26 to 31. Claim 60 Non-living or non-renewable soybean plant material containing soybean event Gm_CSM63717, which is a representative sample of seeds containing soybean event Gm_CSM63717 deposited under ATCC accession number PTA-127604. Claim 61 A product comprising a recombinant DNA molecule of any one of claims 1 to 4 or a DNA construct of any one of claims 26 to 31. Claim 62 In paragraph 61, the product is produced from a transgenic soybean plant, plant part, plant seed, or plant cell containing the soybean event Gm_CSM63717. Claim 63 In paragraph 61 or 62, the product comprises whole or processed seeds; viable or non-viable seeds; viable plant parts (e.g., roots, nodes, buds, or leaves); viable plant cells; processed plant parts; processed plant tissues; dehydrated plant tissues; dehydrated plant parts; frozen plant tissues; frozen plant parts; foods for human consumption such as soybean oil, soy milk, soybean flour, soybean kernels, soybean protein, soybean protein concentrate, hydrolyzed vegetable protein, meat substitutes made from soybean protein, lecithin, curd, tofu, vegetable soybeans (green soybeans), mung bean sprouts, soybean film (yuba), roasted soybeans, miso, tempeh, soy sauce, or natto; plant parts processed into animal feed such as soybean meal; soybean fibers; biodiesel; bio-composite building materials such as particleboard, laminated plywood, or wood products; soybean oil-based solvents; soybean oil-based commercial lubricants; soybean ink; soybean candles; soybean crayons; soybean-based hydraulic fluids; Or a product containing a soy-based foam. Claim 64 A method for producing a commercial product comprising: a) obtaining a transgenic soybean plant, plant part, or plant seed containing the soybean event Gm_CSM63717; and b) producing a commercial product from the transgenic soybean plant, plant part, or plant seed. Claim 65 A method for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds, comprising the step of growing soybean plants in a crop cultivation environment that include (i) a PPO herbicide and (ii) a transgenic gene providing resistance to a herbicide having at least three additional modes of herbicide action, wherein the three additional modes of herbicide action are each different from one another. Claim 66 A method for controlling, preventing, or reducing the occurrence of herbicide-resistant weeds, comprising: a) growing a soybean plant in a crop growing environment comprising the DNA construct of any one of claims 26 to 31, or the event Gm_CSM63717 and at least three additional transgenic genes for providing resistance to a herbicide having at least three additional modes of herbicide action, wherein the three additional modes of herbicide action are each different from each other; and b) applying at least one herbicide selected from the group consisting of dicamba, glufosinate, 2,4-D, PPO inhibitor, β-triketone HPPD inhibitor, glyphosate, and any combination thereof to a crop growing environment, wherein the soybean plant is resistant to at least one herbicide. Claim 67 A method according to claim 65 or 66, wherein a transgenic gene providing resistance to a herbicide having at least three additional modes of herbicide action is present at a single genomic location in a soybean plant. Claim 68 A method for reducing loci for soybean breeding by site-specific insertion of a transgenic gene providing resistance to PPO herbicides at a genomic location within a soybean plant containing a transgenic gene for resistance to at least three additional herbicide modes of action, wherein the three additional herbicide modes of action are each different from each other. Claim 69 A soybean plant, plant seed, plant part, or plant cell, or a method, wherein the additional transgenic gene is selected from the group consisting of FT_Tv7, dicamba monooxygenase (DMO), phosphinothricin N-acetyltransferase (PAT), triketone dioxygenase (TDO), 5-enolpyrubilshikimate-3-phosphate synthesizer (EPSPS), and any combination thereof. Claim 70 In claim 69, the FT_Tv7 transforming gene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 31; the DMO transforming gene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 29; the PAT transforming gene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 27; the TDO transforming gene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 33; and the EPSPS transforming gene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO. 35, a soybean plant, plant seed, plant part, plant cell, or method. Claim 71 A soybean plant, plant seed, plant part, or plant cell, or a method, wherein the additional transgenic gene provides resistance to a herbicide having a mode of action selected from the group consisting of glutamine synthase inhibitors, EPSPS inhibitors, β-triketone HPPD inhibitors, synthetic auxins, and any combination thereof. Claim 72 In claim 71, the synthetic auxin is selected from the group consisting of dicamba, 2,4-D, dichloroprop, mecoprop, 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), and any combination thereof; the glutamine synthase inhibitor comprises glufosinate; the β-triketone HPPD inhibitor is selected from the group consisting of mesotrione, benzobicyclon (BBC), tembotrone, sulfotrione, tefuryltrione, and any combination thereof; and the 5-enolpyrubilchimate-3-phosphate synthase (EPSPS) inhibitor comprises glyphosate, a soybean plant, plant seed, plant part, plant cell, or method. Claim 73 Any one of claims 45 and 69 to 72, or any one of claims 65 to 72, wherein the soybean plant, plant seed, plant part, or plant cell further comprises soybean event MON89788 and / or soybean event Gm_CSM63714, soybean plant, plant seed, plant part, plant cell, or method. Claim 74 In any one of claims 45 and 69 to 72, or any one of claims 65 to 73, the soybean plant, plant seed, plant part, or plant cell is a sequence selected from the group consisting of SEQ ID NO. 182; SEQ ID NO. 183; SEQ ID NO. 184; SEQ ID NO. 185; SEQ ID NO. 186; SEQ ID NO. 187; SEQ ID NO. 188; SEQ ID NO. 189; SEQ ID NO. 190; and SEQ ID NO. 191; A polynucleotide having a sequence identical to at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% of the whole length of SEQ ID NO. 182 or the whole length of SEQ ID NO. 183; and a recombinant DNA molecule further comprising a complete complement of any of the above, a soybean plant, plant seed, plant part, plant cell, or method. Claim 75 Any one of claims 45 and 69 to 74, or any one of claims 65 to 74, wherein the soybean plant, plant seed, plant part, or plant cell is a sequence selected from the group consisting of SEQ ID NO. 192, SEQ ID NO. 193, SEQ ID NO. 194, SEQ ID NO. 195, SEQ ID NO. 196, and SEQ ID NO. 197; A polynucleotide having a sequence identical to at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% of the whole length of SEQ ID NO. 192 or the whole length of SEQ ID NO. 193; and a recombinant DNA molecule further comprising a complete complement of any of the above, a soybean plant, plant seed, plant part, plant cell, or method. Claim 76 A method for controlling or preventing weed growth in a region, comprising the steps of: sowing soybeans in the region containing event Gm_CSM63717, event Gm_CSM63714 and / or event MON89788; and controlling weeds in the region that have no damage to the soybeans or less than 10% damage by applying one or more herbicides selected from the group consisting of PPO herbicides, dicamba, glufosinate, 2,4-D, β-triketone HPPD inhibitors, glyphosate, and any combination thereof.

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