Microbial culture device, microbial sewage treatment assembly including the same, microbial culture method and microbial sewage treatment assembly manufacturing method including the same

Abstract

The present invention relates to a microbial culture device, a microbial wastewater treatment assembly including the same, a method for culturing microorganisms, and a method for manufacturing a microbial wastewater treatment assembly including the same. Specifically, according to one embodiment of the present invention, a microbial culture step performed by a microbial culture device may be provided, comprising: a batch culture step in which sludge is cultured under predetermined batch culture conditions; and a semi-batch culture step in which sludge is cultured under predetermined semi-batch culture conditions, wherein one of the plurality of semi-batch culture unit steps has a concentration of one or more of acetate and nitric acid (NaNO3) different from another of the plurality of semi-batch culture unit steps, and one of the plurality of semi-batch culture unit steps has an input amount of one or more of acetate and nitric acid different from another of the plurality of semi-batch culture unit steps.

Description

Technology Field

[0001] The present invention relates to a microbial culture device, a microbial wastewater treatment assembly including the same, a microbial culture method, and a method for manufacturing a microbial wastewater treatment assembly including the same. Background Technology

[0002] Sewage treatment plants are facilities that treat the inflow of domestic sewage, industrial wastewater, and rainwater to minimize environmental impact and recover water and resources. Sewage treatment plants remove or treat pollutants through various stages and processes. These stages include a pretreatment phase that removes large solids and suspended solids to reduce the burden on subsequent processes; a primary treatment phase that removes suspended solids and some organic matter through sedimentation; a secondary treatment phase that removes pollutants such as organic matter, nitrogen, and phosphorus using microorganisms; a tertiary treatment phase that removes fine particles, residual organic matter, nitrogen, and phosphorus; and a sludge treatment phase that reduces sludge volume, decomposes organic matter, and recovers energy. Due to climate change and urbanization, the volume of sewage inflow is increasing, raising the need to expand sewage treatment capacity. When sewage is treated in a sewage treatment plant, water pollution is prevented and ecosystems are protected, thereby safeguarding the environment. Furthermore, the removal of pathogenic microorganisms prevents waterborne diseases, which can have a positive impact on public health. In addition, biogas can be produced from the wastewater treatment plant and utilized as energy, the separated solids can be used as fertilizer or soil conditioner, and the separated treated water can be used for industrial and landscaping purposes.

[0003] Aeration energy refers to the energy consumed to supply the oxygen required for microorganisms to decompose organic matter during the wastewater treatment process. In wastewater treatment plants, the aeration process plays a critical role in the biological treatment process and accounts for 50% to 60% of total operating energy consumption. The higher the concentration of organic matter and nitrogen in the wastewater, the greater the oxygen required, and the amount of oxygen supplied needs to be regulated according to the activity rate of microorganisms and the treatment capacity. Since aeration energy accounts for a significant portion of wastewater treatment plant operating costs, it is necessary to reduce it through efficient operation.

[0004] In wastewater treatment plants, microorganisms are the primary agents responsible for decomposing organic matter and nutrients contained in wastewater, enabling the process to operate stably despite fluctuations in wastewater inflow and pollutant concentrations that vary with time and season. To ensure a stable supply of microorganisms to the treatment plant, necessary conditions, such as appropriate nutrients, temperature, and oxygen, must be maintained. A problem arises when the conditions required for microbial culture are not met, as microorganisms may not be properly supplied to the plant. Consequently, when the supply of microorganisms becomes unstable, the efficiency of the wastewater treatment plant may decrease. The problem to be solved

[0005] The embodiments of the present invention were invented against the background described above and aim to provide a microbial wastewater treatment assembly in which nitrogen-removing microorganisms are stably supplied while aeration energy is reduced. means of solving the problem

[0006] According to one aspect of the present invention, a microbial culture step performed by a microbial culture device may be provided, comprising: a batch culture step in which sludge is cultured under predetermined batch culture conditions; and a microbial culture step comprising a plurality of semi-batch culture unit steps, wherein the sludge is cultured under predetermined semi-batch culture conditions, and wherein one of the plurality of semi-batch culture unit steps differs from another of the plurality of semi-batch culture unit steps in one or more concentrations of acetate and nitric acid (NaNO3) and one or more amounts of acetate and nitric acid.

[0007] In addition, the plurality of half-batch culture unit steps may include a microbial culture step comprising a first half-batch culture unit step to a 360th half-batch culture unit step.

[0008] In addition, a microbial culture step in which the C / N ratio is measured at the plurality of half-batch culture unit steps may be provided.

[0009] Additionally, the above-mentioned semi-batch culture unit step may include: an input step in which one or more of acetate and nitric acid are introduced into the microbial culture device; a stirring reaction step in which the acetate and nitric acid are stirred in the microbial culture device and nitrogen-removing microorganisms grow; a sedimentation step in which nitrogen-removing microorganisms settle in the microbial culture device; and an outflow step in which the supernatant of the microbial culture device is discharged outside the microbial culture device, and a microbial culture step may be provided.

[0010] In addition, a microbial culture step may be provided in which the time for performing the stirring reaction step is longer than the time for performing the input step, and the time for performing the sedimentation step is longer than the time for performing the stirring reaction step.

[0011] In addition, a microbial culture step may be provided in which the time for performing the stirring reaction step is longer than three times the time for performing the input step and shorter than four times the time for performing the input step.

[0012] In addition, a microbial culture step may be provided, wherein the time for performing the sedimentation step is longer than the time for performing the stirring reaction step and shorter than twice the time for performing the stirring reaction step.

[0013] In addition, it further includes a batch culture step for culturing sludge under batch culture conditions, wherein in the batch culture step, chemical oxygen demand (COD), ammonium (NH4) + A microbial culture step may be provided, wherein the concentrations of ), nitrogen dioxide (NO2), and ammonia (NH3) are measured, and the input step is performed when the concentration of ammonium measured in the batch culture step is less than or equal to a preset input step starting ammonium concentration and the concentration of nitrogen dioxide measured in the batch culture step is less than or equal to a preset input step starting nitrogen dioxide concentration.

[0014] In addition, a method for manufacturing a microbial wastewater treatment assembly performed by a microbial wastewater treatment assembly manufacturing device may be provided, comprising: a step of providing a microbial culture device in which a microbial culture device for culturing nitrogen-removing microorganisms is provided; a step of culturing nitrogen-removing microorganisms in the microbial culture device; and a step of providing a wastewater treatment device in which a wastewater treatment device for treating wastewater using nitrogen-removing microorganisms is provided, wherein the microbial culture step comprises a semi-batch culture step including a plurality of semi-batch culture unit steps, and any one of the plurality of semi-batch culture unit steps differs from another of the plurality of semi-batch culture unit steps in that at least one of the concentrations of acetate and nitric acid and at least one of the amounts of acetate and nitric acid may differ.

[0015] In addition, the step of providing the sewage treatment device includes a step of providing a bioreactor in which organic matter, nitrogen, and phosphorus of sewage are removed using nitrogen-removing microorganisms, and the step of providing the bioreactor includes a predetermined partial nitrification ratio of ammonium to nitrite (NO2 -A method for manufacturing a microbial wastewater treatment assembly may be provided, comprising: a first nitrogen removal step in which the nitrite and ammonium not converted into nitrite are converted into nitrogen (N2); and a second nitrogen removal step in which the nitrite and ammonium not converted into nitrite are converted into nitrogen (N2).

[0016] Additionally, a microbial culture device may be provided, comprising a plurality of semi-batch culture units, wherein one of the plurality of semi-batch culture units differs from another of the plurality of semi-batch culture units in one or more concentrations of acetate and nitric acid and one or more amounts of acetate and nitric acid.

[0017] Additionally, a microbial wastewater treatment assembly may be provided, comprising a microbial culture device for culturing nitrogen-removing microorganisms; and a wastewater treatment device for treating wastewater using nitrogen-removing microorganisms, wherein the microbial culture device comprises a plurality of semi-batch culture units, and one of the plurality of semi-batch culture units differs from another of the plurality of semi-batch culture units in one or more concentrations of acetate and nitric acid and one or more amounts of acetate and nitric acid.

[0018] Additionally, the above sewage treatment device may be provided with a microbial sewage treatment assembly comprising: a screen for filtering debris from the sewage; a grit chamber for separating sand from the sewage; a first sedimentation chamber for separating solids from the sewage; a bioreactor for removing organic matter, nitrogen, and phosphorus (P) from the sewage using nitrogen-removing microorganisms; a second sedimentation chamber for separating sewage sludge and treated water; a disinfection chamber for disinfecting E. coli and viruses; an anaerobic digestion chamber for biogasifying the sewage sludge; a backwash water treatment chamber for removing nitrogen and phosphorus from the sewage; and a dewatering chamber for dewatering the sewage sludge.

[0019] Additionally, the above bioreactor may provide a microbial wastewater treatment assembly comprising: a first nitrogen removal unit that converts ammonium at a preset partial nitrification ratio among ammonium into nitrite; and a second nitrogen removal unit that converts nitrite and ammonium not converted into nitrite into nitrogen.

[0020] Additionally, a microbial sewage treatment assembly may be provided in which the sludge separated from the first sedimentation device and the second sedimentation device flows into the anaerobic digestion device, the solids discharged from the anaerobic digestion device flow into the dewatering device, the return water discharged from the dewatering device flows into the return water treatment device, and the treated return water discharged from the return water treatment device flows into the first sedimentation device. Effects of the invention

[0021] The embodiments of the present invention have the effect of providing a microbial wastewater treatment assembly in which nitrogen-removing microorganisms are stably supplied while aeration energy is reduced. Brief explanation of the drawing

[0022] FIG. 1 is a conceptual diagram of a microbial wastewater treatment assembly according to one embodiment of the present invention. Figure 2 is a microbial culture device of Figure 1. Figure 3 is the sewage treatment device of Figure 1. FIG. 4 is a flowchart of a method for manufacturing a microbial wastewater treatment assembly according to one embodiment of the present invention. Figure 5 is a flowchart of the step of providing the microbial culture device of Figure 4. Figure 6 is a flowchart of the step of providing the input device of Figure 5. Figure 7 is a flowchart of the microbial culture steps of Figure 4. Figure 8 is a flowchart of the semi-batch culture step of Figure 7. Figure 9 is a flowchart of the half-batch culture unit step of Figure 8. Figure 10 is a flowchart of the sewage treatment device providing step of Figure 4. Figure 11 is a flowchart of the bioreactor provision step of Figure 10. Figure 12 is a flowchart of the first nitrogen removal step of Figure 11. Figure 13 is a conceptual diagram of the first nitrogen removal stage and the second nitrogen removal stage. Figure 14 is a photograph of nitrogen-removing microorganisms cultured from sludge sampled from the first region. Figure 15 is a photograph of nitrogen-removing microorganisms cultured from sludge sampled from the second region. Figure 16 is a graph of the C / N ratio and nitrification efficiency according to the experimental period. Specific details for implementing the invention

[0023] Hereinafter, specific embodiments for implementing the technical concept of the present invention will be described in detail with reference to the drawings.

[0024] In addition, in describing the present invention, if it is determined that a detailed description of related known components or functions may obscure the essence of the invention, such detailed description is omitted.

[0025] Furthermore, when it is mentioned that one component is 'supplied' to another component, it should be understood that it may be supplied directly to that other component, or that there may be other components present in between.

[0026] The terms used in this specification are used merely to describe specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

[0027] Furthermore, it should be noted in advance that expressions such as "upper side," "lower side," and "side" in this specification are described based on the drawings, and may be expressed differently if the orientation of the object changes. For the same reason, some components in the attached drawings may be exaggerated, omitted, or schematically depicted, and the size of each component does not entirely reflect its actual size.

[0028] Additionally, terms including ordinal numbers, such as first, second, etc., may be used to describe various components, but such components are not limited by such terms. These terms are used solely for the purpose of distinguishing one component from another.

[0029] The meaning of "comprising" as used in the specification is to specify certain characteristics, regions, integers, steps, actions, elements, and / or components, and does not exclude the existence or addition of other specific characteristics, regions, integers, steps, actions, elements, components, and / or groups.

[0030] Hereinafter, a microbial sewage treatment assembly (1) according to an embodiment of the present invention will be described with reference to FIGS. 1 to 3. The microbial sewage treatment assembly (1) may be a device for treating sewage using nitrogen-removing microorganisms. The microbial sewage treatment assembly (1) may be a device for purifying domestic sewage and industrial wastewater to reduce environmental impact and convert it into reusable water. The microbial sewage treatment assembly (1) may include a microbial culture device (10) and a sewage treatment device (20).

[0031] Referring again to FIG. 2, nitrogen-removing microorganisms can be cultured in the microorganism culture device (10). A semi-batch culture step (S600), which will be described later, can be performed in the microorganism culture device (10). A semi-batch culture step (S600) including a plurality of semi-batch culture unit steps (S610) can be performed in the microorganism culture device (10). The microorganism culture device (10) may include an input device (100), a stirring reaction device (200), and an outflow device (300).

[0032] Raw materials for culturing nitrogen-removing microorganisms may be introduced into the input device (100). The raw materials introduced into the input device (100) may include acetate and nitric acid (NaNO3). The acetate introduced into the input device (100) may provide carbon (C) to the nitrogen-removing microorganisms, and the nitric acid introduced into the input device (100) may provide nitrogen (N) to the nitrogen-removing microorganisms. The input device (100) may include an acetate input device (110) and a nitric acid input device (120).

[0033] Acetate can be introduced into the acetate injection device (110). The acetate injection device (110) can stir the acetate at an acetate stirring speed at a preset acetate temperature condition to achieve a preset acetate concentration. The preset acetate temperature condition may be 20°C or higher and 30°C or lower, and the preset acetate stirring speed may be 90 rpm or higher and 110 rpm or lower. The preset acetate concentration may be 1.4 g / l or higher and 1.6 g / l or lower. For example, the preset acetate temperature condition may be 25°C, the preset acetate stirring speed may be 100 rpm, and the preset acetate concentration may be 1.5 g / l.

[0034] Nitric acid can be injected into the nitric acid injection device (120). The nitric acid injection device (120) can stir the nitric acid at a nitric acid stirring speed at a preset nitric acid temperature condition to achieve a preset nitric acid concentration. The preset nitric acid temperature condition may be 20°C or higher and 30°C or lower, and the preset nitric acid stirring speed may be 90 rpm or higher and 110 rpm or lower. The preset nitric acid concentration may be 45 mg / l or higher and 55 mg / l or lower. For example, the preset nitric acid temperature condition may be 25°C, the preset nitric acid stirring speed may be 100 rpm, and the preset nitric acid concentration may be 50 mg / l.

[0035] In the stirring reaction device (200), raw materials introduced into the input device (100) can be stirred. Acetate stirred in the acetate input device (110) can be introduced into the stirring reaction device (200) in an amount of a preset acetate input, and nitric acid stirred in the nitric acid input device (120) can be introduced in an amount of a preset nitric acid input. The preset acetate input amount may be 45 ml or more and 55 ml or less, and the preset nitric acid input amount may be 450 ml or more and 550 ml or less. For example, the preset acetate input amount may be 50 ml and the preset nitric acid input amount may be 500 ml. When the preset acetate concentration is 1.5 g / l and the preset nitric acid concentration is 50 mg / l, the preset acetate input amount may be 75 mg and the preset nitric acid input amount may be 25 mg. After nitric acid stirred in the nitric acid injection device (120) is injected into the stirring reaction device (200) for a preset nitric acid injection time, acetate stirred in the acetate injection device (110) can be injected for a preset acetate injection time. For example, the preset nitric acid injection time may be 8 minutes or more and 12 minutes or less, and the acetate injection time may be 0.5 minutes or more and 1.5 minutes or less. As a more detailed example, the nitric acid injection time may be 10 minutes and the acetate injection time may be 1 minute. The stirring reaction device (200) may include a sampling unit (210), a batch culture unit (220), and a semi-batch culture unit (230).

[0036] Excess sludge may be sampled in the sampling unit (210). The excess sludge sampled in the sampling unit (210) may contain nitrogen-removing microorganisms. The amount of excess sludge sampled in the sampling unit (210) may be greater than 1 / 6 and less than 1 / 3 of the amount of cultured sludge. In other words, an amount greater than 1 / 6 and less than 1 / 3 of the amount of sludge to be cultured may be sampled in the sampling unit (210).

[0037] In the batch culture section (220), sludge can be cultured under batch culture conditions described later. Batch culture conditions may include batch stirring conditions and batch temperature conditions. After the sludge settles in the batch culture section (220), the supernatant is sampled, and the chemical oxygen demand, ammonium concentration, nitrogen dioxide concentration, and ammonia concentration of the supernatant can be measured.

[0038] In the semi-batch culture section (230), sludge can be cultured under various conditions. In the semi-batch culture section (230), sludge can be cultured under the semi-batch culture conditions described later. The semi-batch culture section (230) may be provided in multiple units. In any one of the multiple semi-batch culture sections (230), the concentration of one or more of the acetate and nitric acid (NaNO3) introduced may differ from that of another of the multiple semi-batch culture sections (230). In any one of the multiple semi-batch culture sections (230), the amount of one or more of the acetate and nitric acid (NaNO3) introduced may differ from that of another of the multiple semi-batch culture sections (230).

[0039] Nitrogen-removing microorganisms can be settled at the bottom of the stirring reaction device (200). The stirring reaction device (200) can be positioned between the acetate injection device (110) and the nitric acid injection device (120). An outflow device (300) can be positioned below the stirring reaction device (200).

[0040] Nitrogen-removing microorganisms settled at the bottom of the stirring reaction device (200) can be discharged to the outside of the microorganism culture device (10) through the discharge device (300). Nitrogen-removing microorganisms settled at the bottom of the stirring reaction device (200) can be discharged through the discharge device (300) as needed at the necessary time. Nitrogen-removing microorganisms discharged to the outside of the microorganism culture device (10) through the discharge device (300) can be introduced into the bioreactor (600) to be described later.

[0041] Referring again to FIG. 3, the sewage treatment device (20) can treat sewage using nitrogen-removing microorganisms. The sewage treatment device (20) may be a device for purifying domestic sewage and industrial wastewater to reduce environmental impact and convert it into reusable water. In the sewage treatment device (20), physical treatment to remove solids and suspended solids, biological treatment to decompose organic matter and pollutants, and chemical treatment to remove micro-pollutants, nitrogen, and phosphorus may be performed. The sewage treatment device (20) may include a screen (400), a grit chamber (500), a first sedimentation chamber (600), a bioreactor (700), a second sedimentation chamber (800), a disinfection device (900), an anaerobic digestion device (1000), a backwash water treatment device (1100), and a dewatering device (1200).

[0042] The screen (400) can filter out impurities from the sewage. The sewage filtered by the screen (400) may be referred to as screen sewage. The screen sewage discharged from the screen (400) may be introduced into the sedimentation device (500).

[0043] The sedimentation device (500) can separate sand from sewage. Screen sewage can be introduced into the sedimentation device (500). Screen sewage from which sand has been separated in the sedimentation device (500) can be named as sedimented sewage.

[0044] The first sedimentation device (600) can separate solids from sewage. Sedimented sewage may flow into the first sedimentation device (600). The first sedimented sewage and raw sludge may be discharged from the first sedimentation device (600). In this specification, sludge may include raw sludge and excess sludge described later. The raw sludge discharged from the first sedimentation device (600) may be a combination of microorganisms and pollutants. Raw sludge may be produced during the process in which microorganisms decompose organic matter in sewage. Raw sludge may be a combination of microorganisms and residues decomposed by microorganisms. The raw sludge discharged from the first sedimentation device (600) may flow into an anaerobic digestion device (1000). The moisture content of the raw sludge may be higher than the moisture content of the excess sludge. The first settling wastewater can be discharged from the first settling device (600) and flow into the bioreactor (700).

[0045] The solids separated in the first sedimentation device (600) may include dissolved solids, suspended solids, volatile solids, and fixed solids.

[0046] The soluble solids separated in the first sedimentation device (600) may be solids dissolved in water that are invisible to the naked eye by combining with water molecules. For example, the soluble solids may include salts and minerals. The suspended solids separated in the first sedimentation device (600) may be solids in the form of particles floating in water that can be separated through physical filtration. For example, the suspended solids may include soil particles and organic matter. The volatile solids separated in the first sedimentation device (600) may be solids that are biologically decomposable and evaporate or decompose upon heating. For example, the volatile solids may include organic matter. The non-volatile solids separated in the first sedimentation device (600) may be inorganic matter that remains after heating. For example, the non-volatile solids may include sand and metal oxides.

[0047] In the bioreactor (700), organic matter, nitrogen, and phosphorus of primary sedimentation wastewater can be removed using nitrogen-removing microorganisms. The first sedimentation wastewater can be introduced into the bioreactor (700). The nitrogen-removing microorganisms placed in the bioreactor (700) may include Azoarcus olearius, Thauera aminoaromatica, and Thauera phenylacetica. The primary sedimentation wastewater from which organic matter, nitrogen, and phosphorus have been removed in the bioreactor (700) may be referred to as bioreactor wastewater. The bioreactor (700) may include a first nitrogen removal unit (710) and a second nitrogen removal unit (720). However, the concept of the present invention is not necessarily limited thereto, and the first nitrogen removal unit (710) and the second nitrogen removal unit (720) may be provided as a single container.

[0048] In the first nitrogen removal unit (710), the first nitrogen removal step (S1010) may be performed. In the first nitrogen removal unit (710), the preset partial nitrification ratio of ammonium may be converted into nitrite. The first nitrogen removal unit (710) may include an ammonia oxidation unit (711), a nitrite oxidation unit (712), and a denitrification unit (713). However, the concept of the present invention is not necessarily limited thereto, and the ammonia oxidation unit (711), the nitrite oxidation unit (712), and the denitrification unit (713) may be provided in a single container.

[0049] In the ammonia oxidation section (711), ammonium and oxygen can be converted into nitrite. The amount of oxygen provided to the ammonia oxidation section (711) may be greater than the amount of oxygen provided to the nitrite oxidation section (712).

[0050] In the nitrite oxidation section (712), the nitrite and oxygen produced in the ammonia oxidation section (711) can be converted into nitrate. In the nitrite oxidation section (712), 0.5 moles of nitrite produced in the ammonia oxidation section (711) can react with oxygen to be converted into 0.5 moles of nitrate.

[0051] In the denitrification section (713), nitrate and carbon generated in the nitrous oxide oxidation section (712) can be converted into nitrite. In the denitrification section (713), 0.5 moles of nitrate generated in the nitrous oxide oxidation section (712) can react with 20% of carbon to be converted into 0.5 moles of nitrite.

[0052] In the second nitrogen removal unit (720), the second nitrogen removal step (S1020) may be performed. In the second nitrogen removal unit (720), nitrite and ammonium that has not been converted into nitrite may be converted into nitrogen.

[0053] In the second sedimentation device (800), sewage sludge and treated water can be separated. Bioreactant sewage can be introduced into the second sedimentation device (800). After the sewage sludge and treated water are separated in the second sedimentation device (800), excess sludge can be introduced into the anaerobic digestion device (1000) together with the raw sludge settled in the first sedimentation device (600). The treated water separated in the second sedimentation device (800) can be introduced into the disinfection device (900). The treated water separated in the second sedimentation device (800) and introduced into the disinfection device (900) can be named as the second sedimented sewage.

[0054] In the disinfection device (900), microorganisms such as E. coli and viruses can be disinfected using ultraviolet rays, ozone, chlorine, etc. The second settling wastewater flowing into the disinfection device (900) may be wastewater from which phosphorus, which was not removed in the bioreactor (700), has been removed in a separate phosphorus removal device (not shown). The second settling wastewater discharged after phosphorus is removed in the disinfection device (900) may be named disinfected wastewater. Disinfected wastewater may be discharged. Disinfected wastewater may be discharged into rivers, lakes, reservoirs, and the sea. Disinfected wastewater may satisfy legal standards for biological oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), and pathogenic microorganisms.

[0055] In the anaerobic digestion device (1000), sewage sludge can be converted into biogas. The anaerobic digestion device (1000) decomposes sludge using anaerobic microorganisms and can recover and utilize the biogas generated during the decomposition process. The biogas generated in the anaerobic digestion device (1000) may contain methane (CH4) gas. The anaerobic digestion device (1000) has the advantage of being able to recover biogas and utilize it as energy. The anaerobic digestion device (1000) may include a closed tank-type digester where anaerobic decomposition takes place, a biogas recovery system for collecting biogas generated within the digester, a sludge discharge system for discharging stabilized sludge or allowing it to be utilized as agricultural fertilizer after undergoing an additional treatment process, and a temperature control system for maintaining a constant temperature. The anaerobic digestion temperature of the anaerobic digestion device (1000) can be maintained through the temperature control system of the anaerobic digestion device (1000). The anaerobic digestion temperature may be between 35°C and 40°C.

[0056] Sludge separated from the first sedimentation device (600) and the second sedimentation device (800) can be introduced into the anaerobic digestion device (1000). Solids discharged from the anaerobic digestion device (1000) can be introduced into the dewatering device (1200).

[0057] Nitrogen and phosphorus in sewage can be removed in the backflow water treatment device (1100). Backflow water containing a relatively high concentration of nitrogen can be introduced into the backflow water treatment device (1100). In the backflow water treatment device (1100), nitrification, nitrification, and denitrification can occur to remove nitrogen. Nitrification is the process in which ammonium nitrate (NO3 - It may be a process of conversion into ). Nitrification is the conversion of ammonium into nitrite (NO2 -It can be carried out by ammonia oxidizing bacteria (AOB) that oxidize into nitrite and nitrite oxidizing bacteria (NOB) that oxidize nitrite into nitrate. Nitrification can be performed under aerobic conditions. Denitrification can be a process in which nitrate is reduced to nitrogen (N2). Denitrification can occur under anaerobic conditions. Nitrification can be a process in which nitrite generated during the nitrification process is further oxidized to become nitrate. The treated backflow water discharged from the backflow water treatment device (1100) can be introduced into the first sedimentation device (600).

[0058] The backflow water treatment device (1100) can be designed to alternately generate aerobic and anaerobic environments. Nitrification can occur in the aerobic environment, and denitrification can occur in the anaerobic environment. The backflow water treatment device (1100) may include an oxygen supply system. When the backflow water treatment device (1100) includes an oxygen supply system, nitrification can be performed in the backflow water treatment device (1100). The backflow water treatment device (1100) may include additives or special microorganisms. The additives or special microorganisms included in the backflow water treatment device (1100) can enable nitrogen to be removed more efficiently.

[0059] The backflow water treatment device (1100) can efficiently remove nitrogen components. When nitrogen components are efficiently removed in the backflow water treatment device (1100), the water quality of the water discharged from the sewage treatment device (20) can be improved, the operating costs of the sewage treatment device (20) can be reduced, and it can contribute to protecting the aquatic ecosystem.

[0060] In the dewatering device (1200), sewage sludge can be dewatered. The backflow water discharged from the dewatering device (1200) can be introduced into the backflow water treatment device (1100). When sewage sludge is dewatered in the dewatering device (1200), the volume and weight of the sludge can be reduced. When the volume and weight of the sludge are reduced, the sludge can be treated more economically. The sludge dewatered in the dewatering device (1200) can be named dewatered sludge. The dewatered sludge can have a moisture content of 75% or more and 85% or less.

[0061] Below, the structure of a sewage treatment device (20) having the configuration described above will be explained.

[0062] Sewage flowing into the sewage treatment device (20) can be fed into the grit chamber (500) after impurities are filtered out at the screen (400). The screened sewage flowing into the grit chamber (500) can be fed into the first sedimentation device (600) after sand is separated. The grit chamber sewage flowing into the first sedimentation device (600) can be separated into raw sludge and first sedimentation sewage. The raw sludge discharged from the first sedimentation device (600) can be fed into the anaerobic digestion device (1000). The first sedimentation sewage discharged from the first sedimentation device (600) can be fed into the bioreactor (700). The first sedimentation sewage from which organic matter, nitrogen, and phosphorus have been removed in the bioreactor (700) can be fed into the second sedimentation device (800). The first settling wastewater flowing into the second settling device (800) can be separated into excess sludge and second settling wastewater. The excess sludge discharged from the second settling device (800) can be introduced into the anaerobic digestion device (1000) together with the raw sludge discharged from the first settling device (600). The second settling wastewater discharged from the second settling device (800) can be introduced into the disinfection device (900). The second settling wastewater can be discharged after phosphorus is chemically removed in the disinfection device (900). The excess sludge discharged from the second settling device (800) and the raw sludge discharged from the first settling device (600) can be introduced into the anaerobic digestion device (1000). In the anaerobic digestion device (1000), raw sludge and excess sludge can be biogasified and the solid matter can be fed into the dewatering device (1200). The backwater discharged from the dewatering device (1200) can be fed into the backwater treatment device (1100) to be treated and then fed into the first sedimentation device (600).

[0063] A method for manufacturing a microbial wastewater treatment assembly (S1) will be described below with reference to FIGS. 4 to 13. The method for manufacturing a microbial wastewater treatment assembly (S1) may be a method for manufacturing a microbial wastewater treatment assembly (1) so that the microbial wastewater treatment assembly (1) can treat wastewater. The method for manufacturing a microbial wastewater treatment assembly (S1) may include a step of providing a microbial culture device (S10), a microbial culture step (S20), and a step of providing a wastewater treatment device (S30).

[0064] Referring again to FIGS. 4 and FIGS. 5, in the step of providing a microbial culture device (S10), a microbial culture device (10) may be provided. In the step of providing a microbial culture device (S10), a microbial culture device (10) in which nitrogen-removing microorganisms provided to a bioreactor (700) are cultured may be provided. The step of providing a microbial culture device (S10) may include a step of providing an input device (S100), a step of providing a stirring reaction device (S200), and a step of providing an outflow device (S300).

[0065] Referring again to FIG. 6, in the step of providing an input device (S100), an input device (100) may be provided. In the step of providing an input device (S100), the input device (100) may be provided in multiple numbers. The step of providing an input device (S100) may include an acetate input device providing step (S110) and a nitric acid input device providing step (S120).

[0066] In the acetate injection device provision step (S110), an acetate injection device (110) may be provided. Acetate may be supplied to the stirring reaction device (200) through the acetate injection device (110) provided in the acetate injection device provision step (S110). The acetate supplied to the stirring reaction device (200) may supply carbon to nitrogen-removing microorganisms.

[0067] In the nitric acid injection device provision step (S120), a nitric acid injection device (120) may be provided. Nitric acid may be supplied to the stirring reaction device (200) through the nitric acid injection device (120) provided in the nitric acid injection device provision step (S120). The nitric acid supplied to the stirring reaction device (200) may supply nitrogen to nitrogen-removing microorganisms.

[0068] Referring again to FIG. 7, nitrogen-removing microorganisms can be cultured in the microbial culture step (S20). The microbial culture step (S20) may include a sampling step (S400), a batch culture step (S500), and a semi-batch culture step (S600).

[0069] In the sampling step (S400), excess sludge may be sampled. The excess sludge sampled in the sampling step (S400) may contain nitrogen-removing microorganisms. The amount of excess sludge sampled in the sampling step (S400) may be greater than 1 / 6 and less than 1 / 3 of the amount of cultured sludge. In other words, in the sampling step (S400), an amount greater than 1 / 6 and less than 1 / 3 of the amount of sludge to be cultured may be sampled.

[0070] In the batch culture step (S500), sludge can be cultured under batch culture conditions. Batch culture conditions may include batch stirring conditions and batch temperature conditions. Batch stirring conditions may be 100 rpm or more and 150 rpm or less, and batch temperature conditions may be 20°C or more and 30°C or less. In the batch culture step (S500), chemical oxygen demand (COD), ammonium concentration, nitrogen dioxide (NO2) concentration, and ammonia (NH3) concentration may be measured. In the batch culture step (S500), after the sludge settles, the supernatant may be sampled, and the chemical oxygen demand, ammonium concentration, nitrogen dioxide concentration, and ammonia concentration of the supernatant may be measured.

[0071] Referring again to FIGS. 8 and 9, in the semi-batch culture step (S600), sludge can be cultured under various conditions. In the semi-batch culture step (S600), sludge can be cultured under preset semi-batch culture conditions. The semi-batch culture step (S600) can be performed at a preset semi-batch culture temperature and a preset semi-batch culture acidity. For example, the preset semi-batch culture temperature may be 25°C or higher and 28°C or lower, and the semi-batch culture acidity may be pH 6.7 or higher and pH 7.2 or lower. In the semi-batch culture step (S600), sludge can be cultured through multiple stages. The semi-batch culture step (S600) may include a plurality of semi-batch culture unit stages (S610).

[0072] In the semi-batch culture unit stage (S610), sludge can be cultured under various conditions. Multiple semi-batch culture unit stages (S610) may be provided. The conversion rate of nitrate (NO3) to nitrogen dioxide and the C / N ratio may be measured in multiple semi-batch culture unit stages (S610). Here, the C / N ratio refers to the ratio of carbon atoms to nitrogen atoms. The C / N ratio may be adjusted differently in multiple semi-batch culture unit stages (S610). For example, in multiple semi-batch culture unit stages (S610), the C / N ratio may be adjusted to increase by 0.1 in the range of 2.3 or more and 3.4 or less. In multiple semi-batch culture unit stages (S610), the C / N ratio at which the conversion rate of nitrate to nitrogen dioxide is highest may be measured. One of the multiple half-batch culture unit stages (S610) may have a different concentration of one or more of acetate and nitric acid compared to another of the multiple half-batch culture unit stages (S610). One of the multiple half-batch culture unit stages (S610) may have a different input amount of one or more of acetate and nitric acid compared to another of the multiple half-batch culture unit stages (S610). When the concentration and input amount of one or more of acetate and nitric acid differ between one of the multiple half-batch culture unit stages (S610) and another of the multiple half-batch culture unit stages (S610), the C / N ratio may be adjusted differently in the multiple half-batch culture unit stages (S610). The half-batch culture unit step (S610) may include a first half-batch culture unit step (S610a), a second half-batch culture unit step (S610b) performed after the first half-batch culture unit step (S610a), and a third half-batch culture unit step (S610c) performed after the second half-batch culture unit step (S610b). However, the concept of the present invention is not limited thereto, and the half-batch culture unit step (S610) may include the first half-batch culture unit step (S610a) to the 360th half-batch culture unit step.The semi-batch culture unit stage (S610) may include an input stage (S611), a stirring reaction stage (S612), a sedimentation stage (S613), and an outflow stage (S614).

[0073] In the input step (S611), one or more of acetate and nitric acid may be input into the microbial culture device (10). The input step (S611) may be performed when the concentration of ammonium measured in the batch culture step (S500) is less than or equal to the preset starting ammonium concentration in the input step, and the concentration of nitrogen dioxide measured in the batch culture step (S500) is less than or equal to the preset starting nitrogen dioxide concentration in the input step. For example, the input step (S611) may be performed for 11 minutes. In the input step (S611), stirred acetate may be input in an amount equal to the preset acetate input amount, and stirred nitric acid may be input in an amount equal to the preset nitric acid input amount. For example, the preset starting nitrogen dioxide concentration in the input step may be 1 mg / l or less.

[0074] In the stirring reaction step (S612), acetate and nitric acid are stirred in the microbial culture device (10), and nitrogen-removing microorganisms can grow. In the stirring reaction step (S612), acetate and nitric acid can be stirred at a speed of 100 rpm or more and 150 rpm or less. The time during which the stirring reaction step (S612) is performed may be longer than the time during which the input step (S611) is performed. For example, the time during which the stirring reaction step (S512) is performed may be longer than three times the time during which the input step (S511) is performed and shorter than four times the time during which the input step (S511) is performed. As a more detailed example, the stirring reaction step (S612) may be performed for 38 minutes.

[0075] In the sedimentation step (S613), nitrogen-removing microorganisms may be settled at the bottom of the microorganism culture device (10). The time during which the sedimentation step (S513) is performed may be longer than the time during which the stirring reaction step (S512) is performed. For example, the time during which the sedimentation step (S513) is performed may be longer than the time during which the stirring reaction step (S512) is performed, and may be shorter than twice the time during which the stirring reaction step (S512) is performed. As a more detailed example, the sedimentation step (S613) may be performed for 60 minutes.

[0076] In the discharge step (S614), the supernatant, which is a substrate excluding acetate and nitric acid used for the growth of nitrogen-removing microorganisms, may be discharged outside the microorganism culture device (10). The supernatant discharged in the discharge step (S614) may be a liquid portion that is not nitrogen-removing microorganisms precipitated inside the microorganism culture device (10).

[0077] Referring again to FIG. 10, in the sewage treatment device providing step (S30), a sewage treatment device (20) may be provided. The method for manufacturing a sewage treatment device (S1) may include a screen providing step (S700), a grit chamber providing step (S800), a first sedimentation device providing step (S900), a biological reactor providing step (S1000), a second sedimentation device providing step (S1100), a disinfection device providing step (S1200), an anaerobic digestion device providing step (S1300), a backwash water treatment device providing step (S1400), and a dehydration device providing step (S1500).

[0078] In the screen provision step (S700), a screen (400) may be provided. The screen (400) provided in the screen provision step (S700) can filter out impurities in the sewage. The impurities filtered by the screen (400) may be larger in size than the substances separated in the sedimentation device (500), the first sedimentation device (600), and the second sedimentation device (800).

[0079] In the sedimentation device provision step (S800), a sedimentation device (500) may be provided. The sedimentation device (500) provided in the sedimentation device provision step (S800) can separate sand from sewage. The sand separated by the sedimentation device (500) may be smaller in size than the debris filtered by the screen (400).

[0080] In the first sedimentation device providing step (S900), a first sedimentation device (600) may be provided. Solids in the sewage may be separated by the first sedimentation device (600) provided in the first sedimentation device providing step (S900).

[0081] Referring again to FIGS. 11 to 13, a bioreactor (700) may be provided in the bioreactor providing step (S1000). Organic matter, nitrogen, and phosphorus of primary sedimentation wastewater may be removed by the bioreactor (700) provided in the bioreactor providing step (S1000). The bioreactor providing step (S1000) may include a first nitrogen removal step (S1010) and a second nitrogen removal step (S1020).

[0082] In the first nitrogen removal step (S1010), a preset partial nitrification ratio of ammonium can be converted into nitrite. For example, the preset partial nitrification ratio may be 40% or more and 60% or less. As a more detailed example, the preset partial nitrification ratio may be 50%. The first nitrogen removal step (S1010) may include an ammonia oxidation step (S1011), a nitrite oxidation step (S1012), and a denitrification step (S1013).

[0083] In the ammonia oxidation step (S1011), ammonium and oxygen can be converted into nitrite. The amount of oxygen provided in the ammonia oxidation step (S1011) may be greater than the amount of oxygen provided in the nitrite oxidation step (S1012). For example, the amount of oxygen provided in the ammonia oxidation step (S1011) may be more than 2 times and less than 4 times the amount of oxygen provided in the nitrite oxidation step (S1012). As a more detailed example, the amount of oxygen provided in the ammonia oxidation step (S1011) may be 3 times the amount of oxygen provided in the nitrite oxidation step (S1012). In the ammonia oxidation step (S1011), 1 mole of ammonium can react with 37.5% oxygen to be converted into 0.5 moles of nitrite. In this specification, % may be the amount when the amount of oxygen and carbon used in each step corresponding to each step of the present invention in the previously disclosed nitrogen removal process, the A2O (anaerobic-anoxic-oxic) process, is assumed to be 100%.

[0084] In the nitrite oxidation step (S1012), the nitrite and oxygen generated in the ammonia oxidation step (S1011) can be converted into nitrate. In the nitrite oxidation step (S1012), 0.5 moles of nitrite generated in the ammonia oxidation step (S1011) can react with 12.5% ​​of oxygen to be converted into 0.5 moles of nitrate.

[0085] In the denitrification step (S1013), nitrate and carbon generated in the nitrous oxide oxidation step (S1012) can be converted into nitrite. In the denitrification step (S1013), 0.5 moles of nitrate generated in the nitrous oxide oxidation step (S1012) can react with 20% of carbon to be converted into 0.5 moles of nitrite.

[0086] In the second nitrogen removal step (S1020), nitrite converted in the first nitrogen removal step (S1010) and ammonium that was not converted into nitrite in the first nitrogen removal step (S1010) can be converted into nitrogen. In the second nitrogen removal step (S1020), anaerobic ammonia oxidation (ANNAMMOX) may occur. In the second nitrogen removal step (S1020), microorganisms such as Candidatus jetenia (Ca.Jettenia), Candidatus brocadia (Ca.Brocadia), Candidatus kuenia (Ca.Kuenenia), Candidatus scalendua (Ca.Scalindua), and Candidatus anammoxosome (Ca.Anammoxosome) may be used. In the second nitrogen removal step (S1020), 0.5 moles of nitrite generated in the denitrification step (S1013) and 0.5 moles of ammonium that was not converted into nitrite in the ammonia oxidation step (S1011) can be converted into nitrogen.

[0087] In the second sedimentation device provision step (S1100), a second sedimentation device (800) may be provided. In the second sedimentation device provision step (S1100), the first sedimented wastewater may be separated into sludge and treated water by the second sedimentation device (800) provided.

[0088] In the disinfection device provision step (S1200), a disinfection device (900) may be provided. Microorganisms such as E. coli and viruses may be disinfected by ultraviolet rays, ozone, chlorine, etc. by the disinfection device (900) provided in the disinfection device provision step (S1200). The second sedimentation wastewater flowing into the disinfection device (900) may be wastewater from which phosphorus has been removed that was not removed in the bioreactor (700) in a separate phosphorus removal device.

[0089] In the anaerobic digestion device provision step (S1300), an anaerobic digestion device (1000) may be provided. Sludge may be biogasified by the anaerobic digestion device (1000) provided in the anaerobic digestion device provision step (S1300).

[0090] In the step of providing a backflow water treatment device (S1400), a backflow water treatment device (1100) may be provided. Nitrogen and phosphorus in sewage may be removed by the backflow water treatment device (1100) provided in the step of providing a backflow water treatment device (S1400).

[0091] In the dehydration device provision step (S1500), a dehydration device (1200) may be provided. The solid material discharged from the anaerobic digestion device (1000) may be dehydrated by the dehydration device (1200) provided in the dehydration device provision step (S1500).

[0092] Hereinafter, with reference to FIGS. 14 to 16, an experiment on a microbial culture step (S20) having the configuration described above will be explained.

[0093] In the sampling step (S400), sludge from the first region and the second region may be sampled and used in the experiment. For example, the first region may be Anseong and the second region may be Tancheon.

[0094] Referring again to FIG. 16, the nitrification efficiency (NTR, Nitrate-to-Nitrite transformation ratio) can be calculated in the microbial culture step (S20). In other words, the ratio of nitrate to nitrite conversion in the microbial culture step (S20) can be calculated. The nitrification efficiency can be calculated by measuring the amounts of nitrogen dioxide and nitrate before and after the microbial culture step (S20). The nitrification efficiency may be the value obtained by dividing the difference between the amount of nitrogen dioxide discharged from the microbial culture device (10) after the microbial culture step (S20) and the amount of nitrogen dioxide supplied to the microbial culture device (10) before the microbial culture step (S20) by the difference between the amount of nitrate discharged from the microbial culture device (10) after the microbial culture step (S20) and the amount of nitrate supplied to the microbial culture device (10) before the microbial culture step (S20). When the C / N ratio is maintained at 2.5 during the microbial culture step (S20), the nitrification efficiency can be measured to be highest.

[0095] When sampling sludge in the first region, nitrogen-removing microorganisms may include one or more of Azoarchus olearius, Tauera aminoaromatica, and Tauera phenylacetica. When sampling sludge in the second region, nitrogen-removing microorganisms may also include one or more of Azoarchus olearius, Tauera aminoaromatica, and Tauera phenylacetica. When sampling sludge in the first region, Azoarchus olearius and Tauera aminoaromatica are cultured in greater quantities than Tauera phenylacetica, but when sampling sludge in the second region, Tauera aminoaromatica may be cultured in greater quantities than Azoarchus olearius and Tauera phenylacetica.

[0096] The effects of the microbial wastewater treatment assembly (1) and the method for manufacturing the microbial wastewater treatment assembly (S1) are described below.

[0097] The microbial wastewater treatment assembly (1) can reduce the aeration energy required for wastewater treatment compared to the existing one. Aeration energy may refer to the energy consumed during the process of supplying oxygen. The existing process may be the well-known A20 (anaerobic-anoxic-oxic) process. The microbial wastewater treatment assembly (1) can reduce the required aeration energy by 50% compared to the existing process. In addition, the microbial wastewater treatment assembly (1) can stably supply nitrogen-removing microorganisms to the bioreactor (700).

[0098] Although the embodiments of the present invention have been described above as specific embodiments, they are merely examples and the present invention is not limited thereto, but should be interpreted as having the broadest scope in accordance with the technical concept disclosed in this specification. Those skilled in the art may implement patterns of shapes not specified by combining or substituting the disclosed embodiments, and this also does not deviate from the scope of the present invention. Furthermore, those skilled in the art may easily modify or alter the disclosed embodiments based on this specification, and it is evident that such modifications or alterations also fall within the scope of the rights of the present invention. Explanation of the symbols

[0099] 1: Microbial wastewater treatment assembly 10: Microbial culture device 100: Input device 110: Acetate injection device 120: Nitric acid injection device 200: Stirring reaction device 210: Sewage treatment device 220: Batch culture section 230: Semi-batch culture section 300: Discharge device 20: Sewage treatment plant 400: Screen 500: Sedimentation device 600: 1st sedimentation device 700: Bioreactor 710: 1st Nitrogen Removal Unit 711: Ammonia Oxidation Unit 712: Nitrous Acid Oxidation Unit 713: Denitrification unit 720: 2nd Nitrogen Removal Unit 800: Second sedimentation device 900: Disinfection device 1000: Anaerobic digestion device 1100: Recirculating water treatment device 1200: Dehydrator S1: Method for manufacturing a microbial wastewater treatment assembly S10: Microbial culture device provision step S100: Input device provision step S110: Acetate injection device provision step S120: Nitric acid injection device provision step S200: Step of providing a stirring reaction device S300: Leakage device provision stage S20: Microbial culture stage S400: Sampling stage S500: Batch culture stage S600: Semi-batch culture stage S610: Semi-batch culture unit stage S610a: 1st semi-batch culture unit stage S610b: Second half-batch culture unit stage S610c: 3rd half-batch culture unit stage S611: Input phase S612: Stirring reaction step S613: Sedimentation stage S614: Leakage stage S30: Sewage treatment device provision stage S700: Screen provision stage S800: Sedimentation device provision stage S900: First sedimentation device provision step S1000: Bioreactor provision step S1010: 1st nitrogen removal stage S1011: Ammonia oxidation step S1012: Nitrite oxidation step S1013: Denitrification stage S1020: 2nd nitrogen removal stage S1100: Second sedimentation device provision step S1200: Disinfection device provision step S1300: Anaerobic digestion device provision step S1400: Step of providing a backflow water treatment device S1500: Dehydration device provision step

Claims

Claim 1 A microbial culture step performed by a microbial culture device, comprising: a batch culture step in which sludge is cultured under predetermined batch culture conditions; and a microbial culture step comprising a plurality of semi-batch culture unit steps, wherein one of the plurality of semi-batch culture unit steps differs from another of the plurality of semi-batch culture unit steps in one or more concentrations of acetate and nitric acid (NaNO3) and one or more amounts of acetate and nitric acid. Claim 2 In claim 1, the plurality of half-batch culture unit steps comprises a microbial culture step including a first half-batch culture unit step to a 360th half-batch culture unit step. Claim 3 In claim 2, a microbial culture step in which the C / N ratio is measured in the plurality of semi-batch culture unit steps. Claim 4 In claim 1, the semi-batch culture unit step comprises: an input step in which one or more of acetate and nitric acid are introduced into the microbial culture device; a stirring reaction step in which the acetate and nitric acid are stirred in the microbial culture device and nitrogen-removing microorganisms grow; a sedimentation step in which nitrogen-removing microorganisms are sedimented in the microbial culture device; and an outflow step in which the supernatant of the microbial culture device is discharged outside the microbial culture device, and the microbial culture step. Claim 5 In claim 4, the time during which the stirring reaction step is performed is longer than the time during which the input step is performed, and the time during which the sedimentation step is performed is longer than the time during which the stirring reaction step is performed, in a microbial culture step. Claim 6 In claim 5, the time during which the stirring reaction step is performed is longer than three times the time during which the input step is performed and shorter than four times the time during which the input step is performed, in a microbial culture step. Claim 7 In claim 5, the time for performing the sedimentation step is longer than the time for performing the stirring reaction step and shorter than twice the time for performing the stirring reaction step, in the microbial culture step. Claim 8 In claim 4, it further includes a batch culture step of culturing sludge under batch culture conditions, wherein in the batch culture step, chemical oxygen demand (COD), ammonium (NH4 + A microbial culture step in which the concentration of ), the concentration of nitrogen dioxide (NO2), and the concentration of ammonia (NH3) are measured, and the input step is performed when the concentration of ammonium measured in the batch culture step is less than or equal to a preset input step starting ammonium concentration and the concentration of nitrogen dioxide measured in the batch culture step is less than or equal to a preset input step starting nitrogen dioxide concentration. Claim 9 A method for manufacturing a microbial wastewater treatment assembly performed by a microbial wastewater treatment assembly manufacturing device, comprising: a step of providing a microbial culture device in which a microbial culture device for culturing nitrogen-removing microorganisms is provided; a step of culturing nitrogen-removing microorganisms in the microbial culture device; and a step of providing a wastewater treatment device in which a wastewater treatment device for treating wastewater using nitrogen-removing microorganisms is provided, wherein the microbial culture step comprises a semi-batch culture step comprising a plurality of semi-batch culture unit steps, wherein one of the plurality of semi-batch culture unit steps differs from another of the plurality of semi-batch culture unit steps in one or more concentrations of acetate and nitric acid and one or more amounts of acetate and nitric acid. Claim 10 In claim 9, the step of providing the sewage treatment device comprises a step of providing a bioreactor in which organic matter, nitrogen, and phosphorus of sewage are removed using nitrogen-removing microorganisms, and the step of providing the bioreactor comprises a preset partial nitrification ratio of ammonium to nitrite (NO2 - A method for manufacturing a microbial wastewater treatment assembly comprising: a first nitrogen removal step in which nitrite and ammonium not converted into nitrite are converted into nitrogen (N2); and a second nitrogen removal step in which nitrite and ammonium not converted into nitrite are converted into nitrogen (N2). Claim 11 A microbial culture device comprising a plurality of semi-batch culture units, wherein one of the plurality of semi-batch culture units differs from another of the plurality of semi-batch culture units in one or more concentrations of acetate and nitric acid and one or more amounts of acetate and nitric acid. Claim 12 A microbial wastewater treatment assembly comprising: a microbial culture device for culturing nitrogen-removing microorganisms; and a wastewater treatment device for treating wastewater using nitrogen-removing microorganisms, wherein the microbial culture device comprises a plurality of semi-batch culture units, and one of the plurality of semi-batch culture units differs from another of the plurality of semi-batch culture units in one or more concentrations of acetate and nitric acid and one or more amounts of acetate and nitric acid. Claim 13 In claim 12, the sewage treatment device comprises: a screen for filtering debris from the sewage; a grit chamber for separating sand from the sewage; a first sedimentation chamber for separating solids from the sewage; a bioreactor for removing organic matter, nitrogen, and phosphorus (P) from the sewage using nitrogen-removing microorganisms; a second sedimentation chamber for separating sewage sludge and treated water; a disinfection device for disinfecting E. coli and viruses; an anaerobic digestion device for biogasifying the sewage sludge; a backwash water treatment device for removing nitrogen and phosphorus from the sewage; and a dewatering device for dewatering the sewage sludge, forming a microbial sewage treatment assembly. Claim 14 In claim 13, the bioreactor comprises: a first nitrogen removal unit that converts ammonium at a preset partial nitrification ratio into nitrite; and a second nitrogen removal unit that converts nitrite and ammonium not converted into nitrite into nitrogen, forming a microbial wastewater treatment assembly. Claim 15 A microbial sewage treatment assembly according to claim 13, wherein the sludge separated from the first sedimentation device and the second sedimentation device flows into the anaerobic digestion device, the solids discharged from the anaerobic digestion device flows into the dewatering device, the return water discharged from the dewatering device flows into the return water treatment device, and the treated return water discharged from the return water treatment device flows into the first sedimentation device.

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