A microneedle patch for skin insertion comprising lactobacillus acidophilus, and a method of manufacturing the same.

Abstract

A method for manufacturing a micro-needle patch for skin insertion according to the present invention may include the steps of: preparing a metal mold having an upper surface in which a plurality of protrusions are arranged two-dimensionally; preparing a polymer solution, a curing agent, and an emulsifier; preparing a lactic acid bacteria culture solution; mixing the polymer solution, the curing agent, and the emulsifier to prepare a polymer mold source solution; providing the polymer mold source solution onto the metal mold to manufacture a polymer mold having a plurality of concave portions having a reverse phase of the plurality of protrusions; and providing a micro-needle source solution containing the lactic acid bacteria culture solution onto the polymer mold having a plurality of concave portions to manufacture a micro-needle having an upper surface in which a plurality of needle portions having a reverse phase of the plurality of concave portions are arranged two-dimensionally.

Description

Technology Field

[0001] The present invention relates to a micro-needle patch for skin insertion comprising Lactobacillus-type lactic acid bacteria and a method for manufacturing the same, and more specifically, to a micro-needle patch for skin insertion comprising a plate portion and a plurality of needle portions including Lactobacillus nagelli, Lactobacillus paragazei, and Lactobacillus bukneri, and a method for manufacturing the same. Background Technology

[0002] A skin-implantable microneedle patch refers to a transdermal drug delivery system that delivers drugs to the human body using micrometer-sized fine needles.

[0003] Micro-needle patches for skin insertion serve to deliver active ingredients to the human body using micrometer-sized needles that penetrate the stratum corneum of the skin.

[0004] Accordingly, micro-needle patches for skin insertion are being utilized in various industries, including small molecule compounds, biological drugs, vaccines, and cosmetics.

[0005] As the fields of application increase, various micro-needle patches for skin insertion are being researched.

[0006] For example, Korean Patent Publication No. 10-1966050 describes a micro-needle patch for skin insertion, comprising: a needle portion having an array of micro-needles for skin insertion and a needle base supporting the array of micro-needles; an active substance portion containing an active substance made of a drug or a functional cosmetic; and a patch portion having a protective patch layer that surrounds the active substance portion and protects the skin after the procedure from the outside, wherein the active substance portion is configured on the skin side relative to the protective patch layer and the needle base is configured on the opposite side of the skin relative to the protective patch layer, so that after the skin insertion procedure of the micro-needles, the needle portion can be separated from the patch portion; further comprising a buffer portion interposed between the needle base and the protective patch layer to protect the array of micro-needles in a manner that surrounds the array of micro-needles and is compressed or collapses to lower its height when the needle portion is pressed; further comprising a first adhesive portion that bonds the needle base and the buffer portion, and a second adhesive portion that bonds the buffer portion and the protective patch layer, wherein the first An integrated micro-needle patch is disclosed in which the adhesive force by the adhesive part is made stronger than the adhesive force by the second adhesive part, so that the buffer part is separated together when the needle part is separated. The problem to be solved

[0007] The technical problem that the present invention aims to solve is to provide a method for manufacturing a micro-needle patch for skin insertion with improved skin protection properties.

[0008] Another technical problem that the present invention aims to solve is to provide a method for manufacturing a micro-needle patch for skin insertion with improved skin wrinkle improvement properties.

[0009] Another technical problem that the present invention aims to solve is to provide a method for manufacturing a micro-needle patch for skin insertion with enhanced anti-inflammatory activity.

[0010] Another technical problem that the present invention aims to solve is to provide a method for manufacturing a micro-needle patch for skin insertion with reduced manufacturing process costs.

[0011] Another technical problem that the present invention aims to solve is to provide a method for manufacturing a micro-needle patch for skin insertion with reduced manufacturing time.

[0012] Another technical problem that the present invention aims to solve is to provide a method for manufacturing a micro-needle patch for skin insertion that is easy to mass-produce.

[0013] The technical problems that the present invention aims to solve are not limited to those described above. means of solving the problem

[0014] To solve the above technical problem, the present invention provides a method for manufacturing a micro-needle patch for skin insertion.

[0015] According to one embodiment, the method for manufacturing the micro-needle patch for skin insertion may include the steps of: preparing a metal mold having an upper surface in which a plurality of protrusions are arranged two-dimensionally; preparing a polymer solution, a curing agent, and an emulsifier; preparing a lactic acid bacteria culture solution; mixing the polymer solution, the curing agent, and the emulsifier to prepare a polymer mold source solution; providing the polymer mold source solution onto the metal mold to manufacture a polymer mold having a plurality of concave portions having a reverse phase of the plurality of protrusions; and providing a micro-needle source solution containing the lactic acid bacteria culture solution onto the polymer mold having a plurality of concave portions to manufacture a micro-needle having an upper surface in which a plurality of needle portions having a reverse phase of the plurality of concave portions are arranged two-dimensionally.

[0016] According to one embodiment, in the step of manufacturing the micro needles, a plurality of the needle portions may have a shape in which the width gradually decreases while extending upward from the upper surface of the metal mold.

[0017] According to one embodiment, the step of preparing the lactic acid bacteria culture solution may include the step of preparing a fermentation solution by mixing grain and wood extracts, the step of preparing lactic acid bacteria, and the step of inoculating the fermentation solution with the lactic acid bacteria.

[0018] According to one embodiment, the lactic acid bacteria may comprise at least one of Lactobacillus gasseri, Leuconostoc kimchii, or Lactobacillus plantarum, and the micro-needle source solution may be prepared by adding at least one of an ascorbic acid derivative, cellulose nanofiber, niacinamide, hyaluronic acid, or collagen to the lactic acid bacteria culture medium.

[0019] According to one embodiment, the step of inoculating the lactic acid bacteria into the fermentation liquid may include multiplying the lactic acid bacteria in the fermentation liquid to produce Lactobacillus nagelli, Lactobacillus paracasei, and Lactobacillus bukneri.

[0020] To solve the above technical problem, the present invention provides a micro-needle patch for skin insertion manufactured according to a method for manufacturing a micro-needle patch for skin insertion.

[0021] According to one embodiment, the micro-needle patch for skin insertion comprises a flat portion, a plurality of needle portions including lactic acid bacteria disposed on the upper surface of the flat portion, and a protective film disposed on the lower surface of the flat portion, wherein the plurality of needle portions are arranged two-dimensionally on the upper surface of the flat portion and have a shape that extends upwardly on the upper surface of the flat portion and has a gradually decreasing width, and the lactic acid bacteria may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus bukneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum. Effects of the invention

[0022] A micro-needle patch for skin insertion according to the present invention may include a micro-needle comprising a flat portion and a plurality of needle portions, and a protective film.

[0023] A plurality of the above needle portions may include Lactobacillus nageli, Lactobacillus paragazei, and Lactobacillus buccneri.

[0024] The above-mentioned Lactobacillus paracasei has the ability to suppress the expression of inflammatory mediators in the skin. As a result, it possesses anticancer and anti-inflammatory functions. Additionally, the above-mentioned Lactobacillus bucceneri has the effect of protecting skin cells from UV exposure, reducing enzymes that cause skin wrinkles, and preventing skin aging.

[0025] Accordingly, when the skin-implantable micro-needle patch comprising a plurality of the above-mentioned needle portions is inserted into the skin, the skin protection properties against UV exposure and the skin wrinkle improvement properties can be enhanced. In addition, the anti-inflammatory activity properties can be enhanced by suppressing the expression of inflammatory mediators in the skin.

[0026] The method for manufacturing the micro-needle patch for skin insertion according to the present invention may include manufacturing a polymer mold to manufacture the micro-needle comprising a plurality of needle portions. When the polymer mold is manufactured using a 3D printer, the manufacturing time of the polymer mold can be shortened compared to when the polymer mold is manufactured using a metal mold. As a result, manufacturing costs are reduced, making it easier to mass-produce the micro-needle patch for skin insertion. Brief explanation of the drawing

[0027] FIG. 1 is a flowchart illustrating a method for manufacturing a micro-needle patch for skin insertion according to an embodiment of the present invention. FIG. 2 is a flowchart illustrating the steps for preparing a lactic acid bacteria culture solution according to an embodiment of the present invention. FIG. 3 is a drawing for explaining a metal mold, a polymer solution, a curing agent, and an emulsifier according to an embodiment of the present invention. FIG. 4 is a drawing for explaining a method for preparing a lactic acid bacteria culture solution according to an embodiment of the present invention. FIG. 5 is a diagram illustrating a method for preparing a micro-needle source solution according to an embodiment of the present invention. FIG. 6 is a diagram illustrating a method for preparing a polymer mold source solution according to an embodiment of the present invention. FIG. 7 is a drawing for explaining a method for manufacturing a polymer mold according to an embodiment of the present invention. FIG. 8 is a drawing for explaining the method of manufacturing a micro-needle patch for skin insertion and the structure of a micro-needle patch for skin insertion according to an embodiment of the present invention. FIG. 9 is a drawing for illustrating a polymer mold according to a first and second modified example of the present invention. FIG. 10 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a first variant of the present invention. FIG. 11 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a first modified example of the present invention. FIG. 12 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a second modified example of the present invention. FIG. 13 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a second modified example of the present invention. FIG. 14 is a drawing for illustrating a polymer mold according to a third and fourth modified example of the present invention. FIG. 15 is a drawing illustrating a method for manufacturing a micro-needle patch for skin insertion according to a third variant of the present invention. FIG. 16 is a drawing illustrating the structure of a micro-needle patch for skin insertion according to a third variant of the present invention. FIG. 17 is a drawing illustrating a method for manufacturing a micro-needle patch for skin insertion according to a fourth variant of the present invention. FIG. 18 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a fourth variant of the present invention. FIG. 19 is a drawing for illustrating a polymer mold according to a fifth modified example of the present invention. FIG. 20 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a fifth variant of the present invention. FIG. 21 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a fifth variant of the present invention. Specific details for implementing the invention

[0028] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the attached drawings. However, the technical concept of the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided to ensure that the disclosed content is thorough and complete and to ensure that the concept of the present invention is sufficiently conveyed to those skilled in the art.

[0029] In this specification, when a component is described as being on another component, it means that it may be formed directly on the other component or that a third component may be interposed between them. Additionally, in the drawings, the thicknesses of the films and regions are exaggerated for the effective description of the technical content.

[0030] Additionally, although terms such as first, second, third, etc., have been used to describe various components in the various embodiments of this specification, these components should not be limited by such terms. These terms are used merely to distinguish one component from another. Accordingly, what is referred to as the first component in one embodiment may be referred to as the second component in another embodiment. Each embodiment described and illustrated herein also includes its complementary embodiment. Furthermore, in this specification, "and / or" is used to mean including at least one of the components listed before and after it.

[0031] In the specification, singular expressions include plural expressions unless the context clearly indicates otherwise. Furthermore, terms such as "include" or "have" are intended to specify the existence of the features, numbers, steps, components, or combinations thereof described in the specification, and should not be understood as excluding the existence or addition of one or more other features, numbers, steps, components, or combinations thereof. Additionally, in this specification, "connection" is used to include both indirectly connecting multiple components and directly connecting them.

[0032] In addition, in describing the present invention below, if it is determined that a detailed description of related known functions or configurations could unnecessarily obscure the essence of the invention, such detailed description will be omitted.

[0034] FIG. 1 is a flowchart for explaining a method for manufacturing a micro-needle patch for skin insertion according to an embodiment of the present invention, FIG. 2 is a flowchart for explaining a step of preparing a lactic acid bacteria culture solution according to an embodiment of the present invention, FIG. 3 is a diagram for explaining a metal mold, a polymer solution, a curing agent, and an emulsifier according to an embodiment of the present invention, FIG. 4 is a diagram for explaining a method for manufacturing a lactic acid bacteria culture solution according to an embodiment of the present invention, FIG. 5 is a diagram for explaining a method for manufacturing a micro-needle source solution according to an embodiment of the present invention, FIG. 6 is a diagram for explaining a method for manufacturing a polymer mold source solution according to an embodiment of the present invention, FIG. 7 is a diagram for explaining a method for manufacturing a polymer mold according to an embodiment of the present invention, and FIG. 8 is a diagram for explaining a method for manufacturing a micro-needle patch for skin insertion and a structure of a micro-needle patch for skin insertion according to an embodiment of the present invention.

[0035] Referring to FIGS. 1 and 3, a metal mold (100), a polymer solution (230), a curing agent (240), and an emulsifier (250) are prepared (S110, S120).

[0036] For example, the polymer solution (230) may be a silicone polymer solution. For example, the curing agent (240) may be a silicone curing agent. For example, the emulsifier (250) may be silicone oil. As described below, a polymer mold source solution (220) may be prepared by mixing the polymer solution (230), the curing agent (240), and the emulsifier (250).

[0037] The metal mold (100) may have an upper surface in which a plurality of protrusions (110) are arranged in a two-dimensional manner. Accordingly, as described below, when the polymer mold source solution (220) is provided on the metal mold (100) and dried to manufacture a polymer mold (200), the polymer mold (200) may have a plurality of concave portions (210) having the reverse phase of the plurality of protrusions (110) of the metal mold (100).

[0038] Referring to FIGS. 1 to 2 and FIG. 4, a lactic acid bacteria culture solution (320) is prepared by mixing a fermentation solution (330) and lactic acid bacteria (340) (S130).

[0039] The step of preparing the above lactic acid bacteria culture solution (320) may include the step of preparing the above fermentation solution (330) by mixing grain and wood extracts (S132), the step of preparing the above lactic acid bacteria (340) (S134), and the step of inoculating the above lactic acid bacteria (340) into the above fermentation solution (330) (S136).

[0040] According to one embodiment, in the step of preparing the fermentation liquid (330), the grain may be in the form of a powder that has been washed with water, roasted at a reference temperature, and ground with a grinder. For example, the grain may be brown rice. For example, the reference temperature may be 220°C.

[0041] In addition, the tree extract may be a diluted extract obtained by providing water equal to 10 times the weight of the tree branch to the tree branch, extracting and filtering at a reference temperature for a reference time, and providing purified water. For example, the tree may be a Rhus verniciflua tree. For example, the reference temperature may be 80°C. For example, the reference time may be 10 hours.

[0042] The above grain and the above wood extract can be mixed and sugar added to produce the above fermented liquid (330). The above fermented liquid (330) serves as a nutrient source for multiplying the above lactic acid bacteria (340).

[0043] In the step of preparing the above lactic acid bacteria (340), for example, the above lactic acid bacteria (340) may be at least one of Lactobacillus gasseri (342), Leuconostoc kimchiai (344), or Lactobacillus plantarum (346).

[0044] According to one embodiment, the Lactobacillus gasseri (342) can be prepared by inoculating refined milk with the Lactobacillus gasseri (342) and culturing it at a reference temperature for a reference time so that the Lactobacillus gasseri (342) has a reference density. For example, the reference temperature may be 34°C. For example, the reference time may be 96 hours. For example, the reference density is 1 x 10⁻⁶ 7 It can be cfu / mL.

[0045] And, the Leuconostoc kimchiaii (344) can be prepared by inoculating the Leuconostoc kimchiaii (344) into the fermentation liquid (300) and culturing it at a reference temperature for a reference time so that the Leuconostoc kimchiaii (344) has a reference density. For example, the reference temperature may be 34℃. For example, the reference time may be 96 hours. For example, the reference density is 1 x 10⁻⁶ 7 It can be cfu / mL.

[0046] Additionally, the Lactobacillus plantanum (346) can be prepared by inoculating the Lactobacillus plantanum (346) into the culture medium (300) and culturing it at a reference temperature for a reference time so that the Lactobacillus plantanum (346) has a reference density. For example, the reference temperature may be 34°C. For example, the reference time may be 96 hours. For example, the reference density is 1 x 10⁶ 7 It can be cfu / mL.

[0047] In the step of inoculating the lactic acid bacteria (340) into the fermentation liquid (330), the lactic acid bacteria (340) inoculated into the fermentation liquid (330) may be at least one of the Lactobacillus gasseri (342), Leuconostoc kimchiai (344), or Lactobacillus plantarum (346), as described above.

[0048] According to one embodiment, the fermented liquid (320) may be inoculated with Lactobacillus gasseri (342), Leuconostoc kimchihai (344), and Lactobacillus plantarum (346).

[0049] After inoculating the above fermentation liquid (330) with the above lactic acid bacteria (340), the above lactic acid bacteria (340) can be multiplied and purified at a standard temperature for a standard time to produce the above lactic acid bacteria culture liquid (320). For example, the standard temperature may be 34℃. For example, the standard time may be 2,160 hours.

[0050] Accordingly, Lactobacillus nagelli, Lactobacillus paracasei, and Lactobacillus bukneri can be produced in the above lactic acid bacteria culture solution (320) by the proliferation of the above lactic acid bacteria (340).

[0051] The above-mentioned Lactobacillus paracasei has the ability to suppress the expression of inflammatory mediators in the skin. As a result, it possesses anticancer and anti-inflammatory functions. Additionally, the above-mentioned Lactobacillus bucceneri has the effect of protecting skin cells from UV exposure, reducing enzymes that cause skin wrinkles, and preventing skin aging.

[0052] Referring to FIGS. 1 and FIGS. 5, a micro needle source solution (310) is prepared by adding an additive to the lactic acid bacteria culture solution (320).

[0053] For example, the additive may be at least one of ascorbic acid derivative (352), cellulose nanofiber (354), niacinamide, hyaluronic acid, or collagen (356).

[0054] The ascorbic acid derivative (352) and the cellulose nanofiber (354) can form hydrogen bonds between the ascorbic acid derivative (352) and the cellulose nanofiber (354). Accordingly, the ascorbic acid derivative (352) and the cellulose nanofiber (354) can form a network structure. As a result, the strength of the micro needle (300) described later can be improved.

[0055] The above niacinamide, the above hyaluronic acid, and the above collagen (356) can improve skin elasticity and skin wrinkles. Also, the above collagen (356) can improve the adhesion between the flat portion (300a) of the micro needle (300) described later and the protective film (400) described later.

[0056] Accordingly, at least one of the ascorbic acid derivative (352), the cellulose nanofiber (354), the niacinamide, the hyaluronic acid, or the collagen (356) may be added to the above lactic acid bacteria culture solution.

[0057] According to one embodiment, the ascorbic acid derivative (352), the cellulose nanofiber (354), and the collagen (356) are added to the lactic acid bacteria culture solution (320) to produce the micro needle source solution (310).

[0058] Referring to FIGS. 1 and 6, the polymer mold source solution (220) is prepared by mixing the polymer solution (230), the curing agent (240), and the emulsifier (250) (S140).

[0059] According to one embodiment, the polymer mold source solution (220) can be prepared by controlling and mixing the weight ratio of the polymer solution (230), the curing agent (240), and the emulsifier (250). For example, the weight ratio of the polymer solution (230), the curing agent (240), and the emulsifier (250) may be 9:1:3.

[0060] Referring to FIGS. 1 and 7, the polymer mold source solution (220) is provided onto the metal mold (100) to manufacture a polymer mold (200) (S150).

[0061] The polymer mold source solution (220) can be provided on the metal mold (100) and dried to produce the polymer mold (200). As described above, the metal mold (100) may have a plurality of protrusions (210). Accordingly, the polymer mold (200) may have a plurality of concave portions (210) having the reverse phase of the plurality of protrusions (110) of the metal mold (100).

[0062] Referring to FIGS. 1 and FIGS. 8, the micro needle source solution (310) is provided to the polymer mold (200) to manufacture the micro needle (300) (S160).

[0063] The micro needle source solution (310) can be provided on the polymer mold (200) and dried to produce the micro needle (300). As described above, the polymer mold (200) may have a plurality of the concave portions (210). Accordingly, a plurality of needle portions (300b) having the reverse phase of the plurality of concave portions (210) of the polymer mold (200) may be produced on the micro needle (300). Additionally, the flat portion (300a) may be formed on the lower surface of the plurality of needle portions (300b).

[0064] A micro needle patch (500) for skin insertion according to the present invention can be manufactured by attaching the protective film (400) to the lower surface of the flat portion (300a) of the micro needle (300).

[0065] Accordingly, the micro needle patch (500) for skin insertion may include the micro needle (300) comprising the flat portion (300a) and a plurality of the needle portions (300b), and the protective film (400).

[0066] Specifically, the plurality of needle portions (300b) may be arranged two-dimensionally on the upper surface of the flat plate portion (300a) and may have a shape that extends upward on the upper surface of the flat plate portion (300a) and has a gradually decreasing width.

[0067] And, the plurality of needle portions (300a) may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus bukneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum derived from the lactic acid bacteria culture (320) in the micro needle source solution (310).

[0068] As described above, the above Lactobacillus paracasei has the ability to suppress the expression of inflammatory mediators in the skin. As a result, it has anticancer and anti-inflammatory functions. In addition, the above Lactobacillus bucceneri has the effect of protecting skin cells against ultraviolet exposure, reducing enzymes that cause skin wrinkles, and preventing skin aging.

[0069] Accordingly, when the skin-implantable micro-needle patch (500) comprising a plurality of the needle portions (300a) is inserted into the skin, the Lactobacillus nageli, Lactobacillus paracasei, and Lactobacillus boucnerie of the plurality of the needle portions (300a) can be easily delivered to the skin. As a result, the skin protection properties against UV exposure and the skin wrinkle improvement properties can be enhanced. In addition, by suppressing the expression of inflammatory mediators in the skin, the active properties for anticancer and anti-inflammatory effects can be enhanced.

[0071] Unlike the embodiments of the present invention described above, according to the first modified embodiment of the present invention, the shape of the polymer mold can be modified so that the micro-needles have a protrusion shape that facilitates the delivery of lactic acid bacteria to the skin. A micro-needle patch for skin insertion according to the first modified embodiment of the present invention is described below with reference to FIGS. 9 to 11.

[0072] FIG. 9 is a drawing for explaining a polymer mold according to a first modified example of the present invention, FIG. 10 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a first modified example of the present invention, and FIG. 11 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a first modified example of the present invention.

[0073] Referring to FIGS. 1 to 6, a polymer mold source solution (220), a lactic acid bacteria culture solution (320), and a micro needle source solution (310) are provided.

[0074] Referring to FIG. 9, the polymer mold source solution (220) can be provided to a 3D printer and 3D printing can be performed to manufacture a polymer mold (202).

[0075] When manufacturing the polymer mold (202) using the above 3D printer, the polymer material can be stacked upward in one pattern at a time within the polymer mold source solution (220).

[0076] Accordingly, the polymer mold (202) may have a first pattern (212), a second pattern (214) having a width narrower than the first pattern (212), a third pattern (216) having a width narrower than the second pattern (214), and a fourth pattern (218) having a width narrower than the third pattern (216).

[0077] Accordingly, a hole (211) may be formed between the edge portions of the first pattern (212) and the second pattern (214), the second pattern (214) and the third pattern (216), and the third pattern (216) and the fourth pattern (218) that are adjacent to each other. Specifically, the cross-section of the edge portions of the first pattern (212), the second pattern (214), the third pattern (216), and the fourth pattern (216) may have a semicircular shape so that the hole (211) may be formed.

[0078] The above hole (211) may gradually decrease in width toward the center at the edges of the first pattern (212), the second pattern (214), the third pattern (216), and the fourth pattern (216).

[0079] Accordingly, the polymer mold source solution (220) is provided to the 3D printer and 3D printing is performed to manufacture the polymer mold (202) having a plurality of holes (211).

[0080] Referring to FIG. 10, the micro needle source solution (310) can be provided on the polymer mold (202) to produce a micro needle (301).

[0081] The micro needle source solution (310) can be provided on the polymer mold (202) having a plurality of the above holes (211) and dried to produce the micro needle (301).

[0082] Accordingly, the micro needle (300) may include a plurality of needle portions (301b) having a plurality of protrusions having a reverse phase of a plurality of holes (211) of the polymer mold (202), and a flat plate portion (301a) on the lower surface of the plurality of needle portions (301b).

[0083] Referring to FIGS. 10 and 11, a micro needle patch (510) for skin insertion can be manufactured by attaching a protective film (400) to the lower surface of the flat portion (301a) of the micro needle (301).

[0084] Accordingly, the micro needle patch (510) for skin insertion may include the micro needle (301) comprising the flat portion (301a) and the plurality of needle portions (301b) having the plurality of protrusions, and the protective film (400).

[0085] Specifically, the plurality of needle portions (301b) having the plurality of protrusions are arranged two-dimensionally on the upper surface of the flat portion (301a) and may have a shape that extends upward on the upper surface of the flat portion (301a) and has a gradually decreasing width.

[0086] Additionally, the plurality of needle portions (301b) having the plurality of the above protrusions may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus bukneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum derived from the lactic acid bacteria culture solution (320) in the micro needle source solution (310) as shown in below.

[0088] 유산균 종류 함유량 % Lactobacillus nagelii group 29.6388 Lactobacillus paracasei group 27.5286 Acetobacter peroxydans group 9.8712 Bacteroides_uc 3.48 Terrimicrobium_uc 2.4785 Unclassified in higher taxonomic rank 1.7346 Lactobacillus buchneri group 1.6059 Micropruina glycogenica 1.5558 Acetobacter lovaniensis group 1.284 AF234694_g_uc 0.7439 Lactobacillus_uc 0.726 PAC002401_s group 0.583 Lactobacillus murinus group 0.5365 PAC000656_s 0.4828 HQ183924_s 0.4399 EF097676_s 0.4256 AY914074_s 0.4185 Bacillus pseudofirmus group 0.3755 Nakamurella multipartita 0.3577 Lactobacillus intestinalis 0.304 PAC001190_s 0.279 Parabacteroides goldsteinii 0.2468 KE159628_s 0.2361 Lactobacillus gasseri group 0.2289 PAC001081_s group 0.2253 HM124124_s 0.2253 AF507695_s 0.2039 PAC001558_s 0.1574 PAC001090_s 0.1574 Oscillibacter_uc 0.1538 Saccharimonas_uc 0.1431 PAC001092_g_uc 0.1395 AF234716_s 0.1395 Mycobacterium abscessus group 0.1359 HQ003485_g_uc 0.1359 Paracoccus denitrificans group 0.1359 Lactobacillus reuteri group 0.1323 Mucispirillum schaedleri 0.1323 Clostridium_uc 0.1323 Hyphomicrobium_uc 0.1323 Micropruina_uc 0.1323 PAC001082_s 0.1288 PAC001126_s 0.1288 PAC001745_s 0.1288 FJ542837_s 0.1288 Bacteroides caccae 0.1252 KE159538_s 0.1216 Saccharimonas aalborgensis 0.1216 PAC001124_s 0.118 EF602869_s 0.1109 PAC001387_s 0.1073

[0089] Accordingly, when the skin-insertable micro-needle patch (510) comprising a plurality of needle portions (301b) having a plurality of the above-mentioned protrusions is inserted into the skin, the Lactobacillus bucceneryi can be easily delivered to the skin by the plurality of the above-mentioned protrusions of the plurality of needle portions (301b). As a result, the skin protection properties against UV exposure, skin wrinkle improvement properties, and active properties for anticancer / anti-inflammatory effects can be improved.

[0090] In addition, the method for manufacturing the micro-needle patch (510) for skin insertion according to the first variant of the present invention may include the step of manufacturing the polymer mold (202) using the 3D printer. Accordingly, the manufacturing time of the polymer mold (202) may be shortened compared to the method of manufacturing the polymer mold (202) using a metal mold. As a result, manufacturing costs are reduced, making it easier to mass-produce the micro-needle patch (510) for skin insertion.

[0092] Unlike the embodiments of the present invention described above, according to a second modified embodiment of the present invention, the shape of the polymer mold may be modified so that the micro-needles have a protrusion shape that facilitates the delivery of lactic acid bacteria to the skin. In addition, a first micro-needle source solution and a second micro-needle source solution with different collagen contents may be prepared so as to improve adhesion between the micro-needles and the protective film. A micro-needle patch for skin insertion according to a second modified embodiment of the present invention is described below with reference to FIG. 9 and FIG. 12 to 13.

[0093] FIG. 9 is a drawing for explaining a polymer mold according to a second modified example of the present invention, FIG. 12 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a second modified example of the present invention, and FIG. 13 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a second modified example of the present invention.

[0094] Referring to FIGS. 1 to 4 and FIG. 6, a polymer mold source solution (220) and a lactic acid bacteria culture solution (320) are provided.

[0095] Referring to FIG. 9, the polymer mold source solution (220) can be provided to a 3D printer and 3D printing can be performed to manufacture a polymer mold (202).

[0096] When manufacturing the polymer mold (202) using the above 3D printer, the polymer material can be stacked upward in one pattern at a time within the polymer mold source solution (220).

[0097] Accordingly, the polymer mold (202) may have a first pattern (212), a second pattern (214) having a width narrower than the first pattern (212), a third pattern (216) having a width narrower than the second pattern (214), and a fourth pattern (218) having a width narrower than the third pattern (216).

[0098] Accordingly, a hole (211) may be formed between the edge portions of the first pattern (212) and the second pattern (214), the second pattern (214) and the third pattern (216), and the third pattern (216) and the fourth pattern (218) that are adjacent to each other. Specifically, the cross-section of the edge portions of the first pattern (212), the second pattern (214), the third pattern (216), and the fourth pattern (216) may have a semicircular shape so that the hole (211) may be formed.

[0099] The above hole (211) may gradually decrease in width toward the center at the edges of the first pattern (212), the second pattern (214), the third pattern (216), and the fourth pattern (216).

[0100] Accordingly, the polymer mold source solution (220) is provided to the 3D printer and 3D printing is performed to manufacture the polymer mold (202) having a plurality of holes (211).

[0101] Referring to FIG. 12, a first micro-needle source solution (310a) is prepared by adding an additive to the lactic acid bacteria culture solution (320), and a second micro-needle source solution (310b) can be prepared in which the ratio of collagen among the additives is lower than that of the first micro-needle source solution (310a). For example, the additive may be at least one of an ascorbic acid derivative, cellulose nanofiber, niacinamide, hyaluronic acid, or collagen.

[0102] A plurality of needle portions (302b) can be manufactured by providing the first micro-needle source solution (310a) on the polymer mold (202) having a plurality of holes (211) and drying it. Then, a flat portion (302a) can be manufactured by providing the second micro-needle source solution (310b) on the plurality of needle portions (302b) and drying it.

[0103] Accordingly, a plurality of needle portions (302b) having a plurality of protrusions having a reverse phase of a plurality of holes (211) of the polymer mold (202) and a second micro needle (302) having a flat plate portion (302a) on the lower surface of the plurality of needle portions (302b) can be manufactured.

[0104] Referring to FIGS. 12 and 13, a micro needle patch (520) for skin insertion can be manufactured by attaching a protective film (400) to the lower surface of the flat portion (302a) of the micro needle (302).

[0105] Accordingly, the micro needle patch (520) for skin insertion may include the micro needle (302) comprising the flat portion (302a) and the plurality of needle portions (302b) having the plurality of protrusions, and the protective film (400).

[0106] Specifically, the plurality of needle portions (302b) having the plurality of protrusions are arranged two-dimensionally on the upper surface of the flat portion (302a) and may have a shape that extends upward on the upper surface of the flat portion (302a) and has a gradually decreasing width.

[0107] Additionally, the plurality of needle portions (302b) having the plurality of the above protrusions may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus bukneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum derived from the lactic acid bacteria culture solution (320) in the first micro needle source solution (310a).

[0108] Accordingly, when the skin-insertable micro-needle patch (520) comprising a plurality of needle portions (302b) having a plurality of the above-mentioned protrusions is inserted into the skin, the Lactobacillus nageli, the Lactobacillus paracasei, and the Lactobacillus bucceneri can be easily delivered to the skin by the plurality of the above-mentioned protrusions of the plurality of needle portions (302b). As a result, the skin protection properties against UV exposure, skin wrinkle improvement properties, and active properties for anticancer / anti-inflammatory effects can be improved.

[0109] In addition, the collagen ratio of the plurality of needle portions (302b) and the plate portion (302a) having the plurality of the above protrusions may be different.

[0110] As described above, a plurality of needle portions (302b) having a plurality of protrusions can be manufactured by the first micro needle source solution (310a), and a flat portion (302a) can be manufactured by the second micro needle source solution (310b).

[0111] As described above, the collagen ratio of the first micro needle source solution (310a) may be lower than that of the second micro needle source solution (310b).

[0112] Accordingly, the collagen content of the above-mentioned flat portion (302a) may be higher than that of the plurality of above-mentioned needle portions (302b).

[0113] As a result, the adhesion between the flat plate (302a) and the protective film (400) is improved, and the structural stability of the micro needle patch (520) for skin insertion can be improved.

[0114] In addition, the method for manufacturing the micro-needle patch (520) for skin insertion according to the second variant of the present invention may include the step of manufacturing the polymer mold (202) using the 3D printer. Accordingly, the manufacturing time of the polymer mold (202) can be shortened compared to the method of manufacturing the polymer mold (202) using a metal mold. As a result, manufacturing costs are reduced, making it easier to mass-produce the micro-needle patch (520) for skin insertion.

[0116] Unlike the embodiments of the present invention described above, according to a third variant of the present invention, the shape of the polymer mold can be modified so that the micro-needles have a protrusion shape that facilitates the delivery of lactic acid bacteria to the skin. A micro-needle patch for skin insertion according to a third variant of the present invention is described below with reference to FIGS. 14 to 16.

[0117] FIG. 14 is a drawing for explaining a polymer mold according to a third modified example of the present invention, FIG. 15 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a third modified example of the present invention, and FIG. 16 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a third modified example of the present invention.

[0118] Referring to FIGS. 1 to 6, a polymer mold source solution (220), a lactic acid bacteria culture solution (320), and a micro needle source solution (310) are provided.

[0119] Referring to FIG. 14, the polymer mold source solution (220) can be provided to a 3D printer and 3D printing can be performed to manufacture a polymer mold (204).

[0120] When the polymer mold (204) is manufactured using the 3D printer, the polymer material of the polymer mold source solution (220) can be stacked upward in a pattern in a first direction.

[0121] Accordingly, the polymer mold (204) may have a first pattern (222), a second pattern (224) having a width narrower than the first pattern (222), a third pattern (226) having a width narrower than the second pattern (224), and a fourth pattern (228) having a width narrower than the third pattern (226).

[0122] Accordingly, a hole (221) may be formed between the edge portions of the first pattern (222) and the second pattern (224), the second pattern (224) and the third pattern (226), and the third pattern (226) and the fourth pattern (228) that are adjacent to each other. Specifically, the cross-section of the edge portions of the first pattern (222), the second pattern (224), the third pattern (226), and the fourth pattern (226) may have a semicircular shape so that the hole (221) may be formed.

[0123] The above hole (221) may gradually decrease in width toward the center from the edges of the first pattern (222), the second pattern (224), the third pattern (226), and the fourth pattern (226).

[0124] Accordingly, the polymer mold source solution (220) is provided to the 3D printer and 3D printing is performed to manufacture the polymer mold (204) having a plurality of holes (221). Then, the polymer mold (204) can be prepared as shown in FIG. 14 by rotating 90 degrees to the right relative to the first direction.

[0125] Referring to FIG. 15, the micro needle source solution (310) can be provided on the polymer mold (204) to produce a micro needle (303).

[0126] The micro needle source solution (310) can be provided on the polymer mold (204) having a plurality of the above holes (221) and dried to produce the micro needle (303).

[0127] Accordingly, the micro needle (303) may include a plurality of needle portions (303b) having a plurality of protrusions having a reverse phase of a plurality of holes (221) of the polymer mold (204), and a flat plate portion (303a) on the lower surface of the plurality of needle portions (303b).

[0128] Referring to FIGS. 15 and 16, a micro needle patch (530) for skin insertion can be manufactured by attaching a protective film (400) to the lower surface of the flat portion (303a) of the micro needle (303).

[0129] Accordingly, the micro needle patch (530) for skin insertion may include the micro needle (303) comprising the flat portion (303a) and the plurality of needle portions (303b) having the plurality of protrusions, and the protective film (400).

[0130] Specifically, the plurality of needle portions (303b) having the plurality of protrusions may be arranged two-dimensionally on the upper surface of the flat portion (303a) and may have a shape in which the width gradually decreases as they extend upwardly on the upper surface of the flat portion (303a). Additionally, the plurality of protrusions of the plurality of needle portions (303b) may gradually narrow in width in the direction in which the micro-needle patch (530) for skin insertion is inserted into the skin.

[0131] And, the plurality of needle portions (303b) having the plurality of the above protrusions may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus bukneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum derived from the lactic acid bacteria culture solution (320) in the micro needle source solution (310).

[0132] Accordingly, when the skin-insertable micro-needle patch (530) comprising a plurality of needle portions (303b) having a plurality of the above-mentioned protrusions is inserted into the skin, the Lactobacillus nageli, the Lactobacillus paracasei, and the Lactobacillus bucceneri can be easily delivered to the skin by the plurality of the above-mentioned protrusions of the plurality of needle portions (303b). As a result, the skin protection properties against UV exposure, skin wrinkle improvement properties, and active properties for anticancer / anti-inflammatory effects can be improved.

[0133] In addition, the method for manufacturing the micro-needle patch (530) for skin insertion according to the third variant of the present invention may include the step of manufacturing the polymer mold (204) using the 3D printer. Accordingly, the manufacturing time of the polymer mold (204) can be shortened compared to the method of manufacturing the polymer mold (204) using a metal mold. As a result, manufacturing costs are reduced, making it easier to mass-produce the micro-needle patch (530) for skin insertion.

[0135] Unlike the embodiments of the present invention described above, according to the fourth variant of the present invention, the shape of the polymer mold may be modified so that the micro-needles have a protrusion shape that facilitates the delivery of lactic acid bacteria to the skin. In addition, a first micro-needle source solution and a second micro-needle source solution with different collagen contents may be prepared so as to improve adhesion between the micro-needles and the protective film. A micro-needle patch for skin insertion according to the fourth variant of the present invention is described below with reference to FIG. 14 and FIG. 17 to 18.

[0136] FIG. 14 is a drawing for explaining a polymer mold according to a fourth modified example of the present invention, FIG. 17 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a fourth modified example of the present invention, and FIG. 18 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a fourth modified example of the present invention.

[0137] Referring to FIGS. 1 to 6, a polymer mold source solution (220), a lactic acid bacteria culture solution (320), and a micro needle source solution (310) are provided.

[0138] Referring to FIG. 14, the polymer mold source solution (220) can be provided to a 3D printer and 3D printing can be performed to manufacture a polymer mold (204).

[0139] When the polymer mold (204) is manufactured using the 3D printer, the polymer material of the polymer mold source solution (220) can be stacked upward in a pattern in a first direction.

[0140] Accordingly, the polymer mold (204) may have a first pattern (222), a second pattern (224) having a width narrower than the first pattern (222), a third pattern (226) having a width narrower than the second pattern (224), and a fourth pattern (228) having a width narrower than the third pattern (226).

[0141] Accordingly, a hole (221) may be formed between the edge portions of the first pattern (222) and the second pattern (224), the second pattern (224) and the third pattern (226), and the third pattern (226) and the fourth pattern (228) that are adjacent to each other. Specifically, the cross-section of the edge portions of the first pattern (222), the second pattern (224), the third pattern (226), and the fourth pattern (226) may have a semicircular shape so that the hole (221) may be formed.

[0142] The above hole (221) may gradually decrease in width toward the center from the edges of the first pattern (222), the second pattern (224), the third pattern (226), and the fourth pattern (226).

[0143] Accordingly, the polymer mold source solution (220) is provided to the 3D printer and 3D printing is performed to manufacture the polymer mold (204) having a plurality of holes (221). Then, the polymer mold (204) can be prepared as shown in FIG. 14 by rotating 90 degrees to the right relative to the first direction.

[0144] Referring to FIG. 17, a first micro-needle source solution (310a) is prepared by adding an additive to the lactic acid bacteria culture solution (320), and a second micro-needle source solution (310b) can be prepared in which the ratio of collagen among the additives is lower than that of the first micro-needle source solution (310a). For example, the additive may be at least one of an ascorbic acid derivative, cellulose nanofiber, niacinamide, hyaluronic acid, or collagen.

[0145] A plurality of needle portions (304b) can be manufactured by providing the first micro-needle source solution (310a) on the polymer mold (204) having a plurality of holes (221) and drying it. Then, a flat portion (304a) can be manufactured by providing the second micro-needle source solution (310b) on the plurality of needle portions (304b) and drying it.

[0146] Accordingly, a micro needle (304) may be manufactured including a plurality of needle portions (304b) having a plurality of protrusions having a reverse phase of a plurality of holes (221) of the polymer mold (204), and a flat plate portion (304a) on the lower surface of the plurality of needle portions (304b).

[0147] Referring to FIGS. 17 and 18, a micro needle patch (540) for skin insertion can be manufactured by attaching a protective film (400) to the lower surface of the flat portion (304a) of the micro needle (304).

[0148] Accordingly, the micro needle patch (540) for skin insertion may include the micro needle (304) comprising the flat portion (304a) and the plurality of needle portions (304b) having the plurality of protrusions, and the protective film (400).

[0149] Specifically, the plurality of needle portions (304b) having the plurality of protrusions may be arranged two-dimensionally on the upper surface of the flat portion (304a) and may have a shape in which the width gradually decreases as they extend upwardly on the upper surface of the flat portion (304a). Additionally, the plurality of protrusions of the plurality of needle portions (304b) may gradually narrow in width in the direction in which the micro-needle patch (540) for skin insertion is inserted into the skin.

[0150] Additionally, the plurality of needle portions (304b) having the plurality of the above protrusions may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus bukneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum derived from the lactic acid bacteria culture solution (320) in the first micro needle source solution (310a).

[0151] Accordingly, when the skin-insertable micro-needle patch (540) comprising a plurality of needle portions (304b) having a plurality of the above-mentioned protrusions is inserted into the skin, the Lactobacillus nageli, the Lactobacillus paracasei, and the Lactobacillus bucceneri can be easily delivered to the skin by the plurality of the above-mentioned protrusions of the plurality of needle portions (304b). As a result, the skin protection properties against UV exposure, skin wrinkle improvement properties, and active properties for anticancer / anti-inflammatory effects can be improved.

[0152] In addition, the collagen ratio of the plurality of needle portions (304b) and the plate portion (304a) having the plurality of the above protrusions may be different.

[0153] As described above, a plurality of needle portions (304b) having a plurality of protrusions can be manufactured by the first micro needle source solution (310a), and a flat portion (304a) can be manufactured by the second micro needle source solution (320b).

[0154] As described above, the collagen ratio of the first micro needle source solution (310a) may be lower than that of the second micro needle source solution (310b).

[0155] Accordingly, the collagen content of the above-mentioned flat portion (304a) may be higher than that of the plurality of above-mentioned needle portions (304b).

[0156] As a result, the adhesion between the flat plate (304a) and the protective film (400) is improved, and the structural stability of the micro needle patch (540) for skin insertion can be improved.

[0157] In addition, the method for manufacturing the micro-needle patch (540) for skin insertion according to the fourth variant of the present invention may include the step of manufacturing the polymer mold (204) using the 3D printer. Accordingly, the manufacturing time of the polymer mold (204) can be shortened compared to the method of manufacturing the polymer mold (204) using a metal mold. As a result, manufacturing costs are reduced, making it easier to mass-produce the micro-needle patch (540) for skin insertion.

[0159] Unlike the embodiments of the present invention described above, according to the fifth variant of the present invention, the shape of the polymer mold may be modified so that the micro-needles have a protrusion shape that facilitates the delivery of lactic acid bacteria to the skin, with a number greater than the standard number. A micro-needle patch for skin insertion according to the fifth variant of the present invention is described below with reference to FIGS. 19 to 21.

[0160] FIG. 19 is a drawing for explaining a polymer mold according to a fifth variant of the present invention, FIG. 20 is a drawing for explaining a method for manufacturing a micro-needle patch for skin insertion according to a fifth variant of the present invention, and FIG. 21 is a drawing for explaining the structure of a micro-needle patch for skin insertion according to a fifth variant of the present invention.

[0161] Referring to FIGS. 1 to 6, a polymer mold source solution (220), a lactic acid bacteria culture solution (320), and a micro needle source solution (310) are provided.

[0162] Referring to FIG. 19, the polymer mold source solution (220) can be provided to a 3D printer and 3D printing can be performed to manufacture a polymer mold (206).

[0163] When the polymer mold (206) is manufactured using the 3D printer, the polymer material of the polymer mold source solution (220) can be stacked upward in a pattern in a first direction.

[0164] Accordingly, the polymer mold (206) may have a first pattern (232), a second pattern (233) having a narrower width and thinner thickness than the first pattern (232), a third pattern (234) having a narrower width and thinner thickness than the second pattern (233), a fourth pattern (235) having a narrower width and thinner thickness than the third pattern (234), a fifth pattern (236) having a narrower width and thinner thickness than the fourth pattern (235), and a sixth pattern (237) having a narrower width and thinner thickness than the fifth pattern (236).

[0165] The first pattern (232) may be thicker than the reference thickness. Therefore, the number of 3D printing steps may be reduced compared to when the first pattern (233) is 3D printed thinner than the reference thickness. As a result, the manufacturing time of the polymer mold (206) may be reduced. Accordingly, the manufacturing cost of the polymer mold (206) is reduced, making it easier to mass-produce the micro-needle patch (550) for skin insertion described later.

[0166] In contrast, the second pattern (233) to the sixth pattern (237) may be thinner than the reference thickness. Accordingly, the number of protrusions of the micro needle (305) described later may be greater than the reference number. As a result, the resolution of the needle part (305b) described later having the number of protrusions may be improved.

[0167] And, a hole (231) may be formed between the edge portions of the first pattern (232) and the second pattern (233), the second pattern (223) and the third pattern (224), the third pattern (234) and the fourth pattern (235), the fourth pattern (235) and the fifth pattern (236), and the fifth pattern (236) and the sixth pattern (237) that are adjacent to each other.

[0168] The hole (231) may be formed such that the cross-section of the edge portion of the first pattern (232), the second pattern (233), the third pattern (234), the fourth pattern (235), the fifth pattern (236), and the sixth pattern (237) has a semicircular shape.

[0169] Additionally, the width of the hole (231) may gradually decrease from the edge portions of the first pattern (232), the second pattern (233), the third pattern (234), the fourth pattern (235), the fifth pattern (236), and the sixth pattern (237) toward the center.

[0170] Accordingly, the polymer mold source solution (220) is provided to the 3D printer and 3D printing is performed to manufacture the polymer mold (206) having a plurality of holes (231). Then, the polymer mold (206) can be prepared as shown in FIG. 19 by rotating 90 degrees to the right relative to the first direction.

[0171] Referring to FIG. 20, the micro needle source solution (310) can be provided on the polymer mold (206) to produce the micro needle (305).

[0172] The micro needle source solution (310) can be provided on the polymer mold (206) having a plurality of the above holes (231) and dried to produce the micro needle (305).

[0173] Accordingly, the micro needle (305) may include a plurality of needle portions (305b) having a plurality of protrusions having a reverse phase of a plurality of holes (231) of the polymer mold (206), and a flat plate portion (305a) on the lower surface of the plurality of needle portions (305b).

[0174] Referring to FIGS. 20 and 21, a micro needle patch (550) for skin insertion can be manufactured by attaching a protective film (400) to the lower surface of the flat portion (305a) of the micro needle (305).

[0175] Accordingly, the micro needle patch (550) for skin insertion may include the micro needle (305) comprising the flat portion (305a) and the plurality of needle portions (305b) having the plurality of protrusions, and the protective film (400).

[0176] Specifically, the plurality of needle portions (305b) having the plurality of protrusions may be arranged two-dimensionally on the upper surface of the flat portion (305a) and may have a shape that extends upward on the upper surface of the flat portion (305a) and has a gradually decreasing width. In addition, the plurality of needle portions (305b) having the plurality of protrusions may include at least one of Lactobacillus nagelli, Lactobacillus paracasei, Lactobacillus boucneri, Lactobacillus gasseri, Leuconostoc kimchiai, or Lactobacillus plantanum derived from the lactic acid bacteria culture solution (320) within the micro-needle source solution (310).

[0177] Additionally, the width of the plurality of protrusions of the plurality of needle portions (305b) may gradually narrow in the direction in which the micro-needle patch (530) for skin insertion is inserted into the skin. And, as described above, the plurality of protrusions may have a number greater than the reference thickness, such that the second pattern (233) to the sixth pattern (237) are formed thinner than the reference thickness. As a result, the resolution of the plurality of needle portions (305b) may be improved. Accordingly, the micro-needle patch (550) for skin insertion including the needle portion (305b) having the plurality of protrusions can be easily inserted into the skin. Furthermore, the Lactobacillus nageli, the Lactobacillus paracasei, and the Lactobacillus bucceneri can be easily delivered to the skin by the plurality of protrusions (greater than the reference number) of the plurality of needle portions (306b). As a result, skin protection properties against UV exposure, skin wrinkle improvement properties, and active properties for anticancer / anti-inflammatory effects can be enhanced.

[0179] Although the present invention has been described in detail using preferred embodiments, the scope of the invention is not limited to specific embodiments and should be interpreted by the appended claims. Furthermore, those skilled in the art will understand that many modifications and variations are possible without departing from the scope of the invention. Explanation of the symbols

[0180] 100: Metal mold 110: Protrusion 220: Polymer mold source solution 310: Microneedle source solution 300: Micro needles 300a: Flat plate 300b: Needle part 400: Protective film 500: Micro-needle patch for skin insertion

Claims

Claim 1 The method comprises the steps of: preparing a polymer solution, a curing agent, and an emulsifier; preparing a lactic acid bacteria culture solution; mixing the polymer solution, the curing agent, and the emulsifier to prepare a polymer mold source solution; providing the polymer mold source solution to a 3D printer to manufacture a polymer mold having a plurality of concave portions having a reverse phase of a plurality of protrusions; and providing a micro needle source solution, prepared by adding an additive to the lactic acid bacteria culture solution, onto the polymer mold having a plurality of concave portions to manufacture a micro needle having an upper surface in which a plurality of needle portions having a reverse phase of a plurality of concave portions are arranged two-dimensionally, wherein the polymer mold comprises: a first pattern; a second pattern having a narrower width than the first pattern and a thinner thickness than the first pattern; and a third pattern having a narrower width than the second pattern and a thickness equal to or thinner than the second pattern. A fourth pattern having a width narrower than the third pattern and a thickness equal to or thinner than the third pattern; a fifth pattern having a width narrower than the fourth pattern and a thickness equal to or thinner than the fourth pattern; and a sixth pattern having a width narrower than the fifth pattern and a thickness equal to or thinner than the fifth pattern, wherein the first pattern, the second pattern, the third pattern, the fourth pattern, the fifth pattern, and the sixth pattern formed by stacking in a first direction are prepared by rotating 90 degrees, and the cross-sections of the edges of the first pattern, the second pattern, the third pattern, the fourth pattern, the fifth pattern, and the sixth pattern adjacent to each other have a semicircular shape, and holes are formed between the edges of the first pattern, the second pattern, the third pattern, the fourth pattern, the fifth pattern, and the sixth pattern adjacent to each other, and the micro needle source solution is a first micro needle source solution;A method for manufacturing a micro-needle patch for skin insertion, comprising: a second micro-needle source solution to which an additive with a higher proportion of collagen than the additive added to the first micro-needle source solution is added; and a step of manufacturing the micro-needles comprises: a step of providing the first micro-needle source solution onto a polymer mold having a plurality of holes and drying it to produce a plurality of needle portions having a plurality of protrusions having a reverse phase of the holes; a step of providing the second micro-needle source solution onto the plurality of needle portions and drying it to produce a flat portion; and a step of attaching a protective film to the lower surface of the flat portion. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete

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