Ceramide carrier manufacturing method using hydrophobic liquid crystal and hydrophobic liquid crystal-based ceramide carrier according thereto

Abstract

The present invention discloses a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal and a hydrophobic liquid crystal-based ceramide carrier according to the same, comprising: A) a step of uniformly mixing a hydrophobic liquid crystal, a ceramide, and a surfactant to produce a mixture; B) a step of dispersing the mixture in water to form liquid crystal microdroplets; and C) a step of self-assembling the ceramide at the droplet interface and supporting it inside the droplet.

Description

Technology Field

[0001] The present invention relates to a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal and a hydrophobic liquid crystal-based ceramide carrier according to the same. Background Technology

[0003] Ceramide is one of the components that make up the skin barrier and plays a role in preventing moisture evaporation from the skin. Modern society has seen advancements in hygiene standards compared to the past, leading to a culture of frequent washing. Consequently, the habit of washing often causes ceramide to leak out of the skin, resulting in dry skin and causing various skin diseases. Since the amount of ceramide naturally produced in the body is insufficient to replenish the deficiency, it is crucial to supply additional ceramide through moisturizers.

[0004] However, due to structural issues, ceramides cannot be incorporated in large quantities into aqueous moisturizers. Ceramides have an amphiphilic structure consisting of a hydrophilic head and a hydrophobic tail. Consequently, they exhibit low solubility in aqueous solutions due to the hydrophobicity of their tails. Furthermore, hydrogen bonding in the hydrophilic heads generates strong intermolecular forces, leading to the formation of aggregates that induce crystallization within the solution. To address the low stability and poor dispersibility of ceramides in aqueous solutions resulting from these characteristics, it is crucial to develop a carrier capable of carrying the ceramides.

[0005] Conventionally, ceramides were loaded using hydrophilic liquid crystals (lyotropic liquid crystals) as carriers. When a lipid component with an amphiphilic structure is added to an aqueous solution at a concentration above a certain level, microdroplets having a hydrophilic liquid crystal phase are formed within the aqueous solution. When ceramide is added at this time, the (amphiphilic) ceramide molecules self-assemble among the lipid molecules of the microdroplets due to the affinity between the ceramide and lipid molecules (left side of Fig. 1). Consequently, direct interactions between ceramides are reduced, which suppresses crystallization and allows for the loading of approximately 3 wt% of ceramide.

[0006] However, the aforementioned hydrophilic liquid crystals used as conventional ceramide carriers react sensitively to external stimuli, causing their properties to change and resulting in reduced stability. For example, the evaporation of the aqueous solution induces phase transitions in the microdroplets, causing them to lose their carrier properties. Furthermore, due to the presence of a hydrophilic (hydrophilic liquid crystal droplet)-hydrophilic (aqueous solution) interface, the energy barrier for carriers to combine is low, leading to significantly poor dispersibility.

[0007] In other words, since existing hydrophilic liquid crystal-based ceramide carrier technology suffers from low stability, low dispersibility, and low loading concentration, a new ceramide carrier technology is required to solve these problems. The problem to be solved

[0009] The hydrophobic liquid crystal-based ceramide carrier proposed in this invention is a technology that utilizes hydrophobic liquid crystals to enable the loading of high concentrations of ceramide with high stability and dispersibility.

[0010] Meanwhile, the technical problems to be solved by the present invention are not limited to those mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art to which the present invention belongs from the description below. means of solving the problem

[0012] An embodiment of the present invention may provide a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal, comprising: A) a step of uniformly mixing a hydrophobic liquid crystal, a ceramide, and a surfactant to produce a mixture; B) a step of dispersing the mixture in water to form liquid crystal microdroplets; and C) a step of self-assembly of the ceramide at the droplet interface and internal loading of the droplets.

[0013] In addition, a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal can be provided, wherein in step A) above, the mixing ratio of the hydrophobic liquid crystal, ceramide, and surfactant is 100 to 50 (excluding 100) : 0 to 25 (excluding 0) : 0 to 25 (excluding 0) by weight ratio.

[0014] In addition, the above hydrophobic liquid crystal may provide a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal comprising at least one of cholesteryl oleyl carbonate, cholesteryl nonanoate, and cholesteryl chloride.

[0015] In addition, the above hydrophobic liquid crystal may provide a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal in which the mixing ratio of cholesteryl oleyl carbonate and cholesteryl chloride is 100 to 65 (excluding 100) : 0 to 35 (excluding 0) by weight, or the mixing ratio of cholesteryl oleyl carbonate and cholesteryl nonanoate is 100 to 70: 0 to 30 by weight, or the mixing ratio of cholesteryl isostearate and cholesteryl chloride is 100 to 75: 0 to 25 by weight, or the mixing ratio of cholesteryl oleate and cholesteryl chloride is 100 to 75: 0 to 25 by weight.

[0016] In addition, the above surfactant may provide a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal comprising at least one of lauric acid, myristic acid, palmitic acid, stearic acid, polyglyceryl-10 stearate, polyglyceryl-6 stearate, polyglyceryl-10 laurate, and glyceryl monostearate.

[0017] In addition, the above surfactant can provide a method for manufacturing a ceramide carrier using a hydrophobic liquid crystal in which the mixing ratio of glyceryl stearate and stearic acid is 50:50 by weight.

[0018] Additionally, a hydrophobic liquid crystal-based ceramide carrier can be provided, comprising a liquid crystal microdroplet formed by dispersing a mixture including a hydrophobic liquid crystal, ceramide, and a surfactant in water, wherein some of the ceramide self-assembles at the droplet interface and some is supported inside the droplet.

[0019] In addition, a hydrophobic liquid crystal-based ceramide carrier can be provided in which the mixing ratio of the hydrophobic liquid crystal, ceramide, and surfactant in the above mixture is 100 to 50 (excluding 100) : 0 to 25 (excluding 0) : 0 to 25 (excluding 0) by weight.

[0020] In addition, the hydrophobic liquid crystal may provide a hydrophobic liquid crystal-based ceramide carrier comprising at least one of cholesteryl oleyl carbonate, cholesteryl nonanoate, and cholesteryl chloride.

[0021] In addition, the hydrophobic liquid crystal may provide a hydrophobic liquid crystal-based ceramide carrier in which the mixing ratio of cholesteryl oleyl carbonate and cholesteryl chloride is 100-65:0-35 by weight, or the mixing ratio of cholesteryl oleyl carbonate and cholesteryl nonanoate is 100-70:0-30 by weight, or the mixing ratio of cholesteryl isostearate and cholesteryl chloride is 100-75:0-25 by weight, or the mixing ratio of cholesteryl oleate and cholesteryl chloride is 100-75:0-25 by weight.

[0022] In addition, the surfactant may provide a hydrophobic liquid crystal-based ceramide carrier comprising at least one of lauric acid, myristic acid, palmitic acid, stearic acid, polyglyceryl-10 stearate, polyglyceryl-6 stearate, polyglyceryl-10 laurate, and glyceryl monostearate.

[0023] In addition, the above surfactant can provide a hydrophobic liquid crystal-based ceramide carrier in which the mixing ratio of glyceryl stearate and stearic acid is 50:50 by weight. Effects of the invention

[0025] According to an embodiment of the present invention, a high concentration of ceramide can be loaded with high stability and dispersibility by using a hydrophobic liquid crystal.

[0026] Meanwhile, the effects obtainable from the present invention are not limited to those mentioned above, and other unmentioned effects will be clearly understood by those skilled in the art to which the present invention belongs from the description below. Brief explanation of the drawing

[0028] Figure 1 is a conceptual diagram comparing hydrophilic liquid crystal carriers and hydrophobic liquid crystal carriers. Figure 2 shows photographs of hydrophobic liquid crystal-based ceramide carriers observed through a polarizing microscope, where (a) is a photograph of a liquid crystal droplet without ceramide and (b) is a photograph of a hydrophobic liquid crystal-based ceramide carrier. Figure 3 is a graph showing the surface self-assembly characteristics of ceramide, (a) showing the self-assembly characteristics at the water interface when ceramide and a surfactant are mixed with mineral oil, a representative hydrophobic oil, and E7, a representative hydrophobic liquid crystal. (b) is a graph comparing the loading amount (black) when ceramide is self-assembled only at the interface of the hydrophobic liquid crystal-based ceramide carrier and the loading amount from actual experimental results (red). Figure 4 shows a graph and photographs illustrating the internal structure formation of ceramide within a hydrophobic liquid crystal, (a) is a graph showing the change in phase transition temperature and brightness of the hydrophobic liquid crystal as the concentration of ceramide and surfactant increases, and (b), (c), and (d) are polarized microscope photographs at each concentration. Figure 5 shows fluorescence confocal microscope images of hydrophobic liquid crystal-based ceramide carriers, where (a) is a fluorescence image of a liquid crystal droplet without ceramide and (b) is a fluorescence image of a hydrophobic liquid crystal-based ceramide carrier. Figure 6 is a graph and photograph analyzing the internal structure of a hydrophobic liquid crystal-based ceramide carrier, (a) is the result of wide-angle X-acid scattering analysis, (b) is a polarized microscope image of a hydrophobic liquid crystal droplet, (c) is a polarized microscope image of a mixed liquid crystal of ceramide and surfactant, and (d) is a polarized microscope image of a hydrophobic liquid crystal-based ceramide carrier liquid crystal. Figure 7 shows a hydrophobic liquid crystal-based ceramide carrier loaded with 10 wt% ceramide, (a) a polarized light microscope image showing dispersibility, and (b) a vial image. Figure 8 is a graph showing the change in droplet size after storing a hydrophobic liquid crystal-based ceramide carrier loaded with 10 wt% ceramide for 17 days. Specific details for implementing the invention

[0029] Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings. Embodiments of the present invention may be modified in various forms, and the scope of the present invention should not be interpreted as being limited to the embodiments below. These embodiments are provided to more fully explain the present invention to those with average knowledge in the art. Accordingly, the shapes of the elements in the drawings have been exaggerated to emphasize clearer explanations.

[0030] The configuration of the invention to clarify the solution to the problem to be solved by the present invention is described in detail with reference to the attached drawings based on preferred embodiments of the present invention. In assigning reference numbers to the components of the drawings, the same reference number is assigned to identical components even if they are located in different drawings, and it is noted in advance that components of other drawings may be cited if necessary when describing the drawings.

[0031] Figure 1 is a conceptual diagram comparing hydrophilic liquid crystal carriers and hydrophobic liquid crystal carriers.

[0032] As shown in Figure 1, the characteristics (hydrophobicity) of the hydrophobic liquid crystal-based ceramide carrier are different from the characteristics (hydrophilicity) of the existing carrier, thereby improving the stability and dispersibility of the carrier.

[0033] Unlike conventional hydrophilic liquid crystal carriers, hydrophobic liquid crystal carriers have enhanced stability, meaning the liquid crystal properties do not change due to the evaporation of the solvent (aqueous solution).

[0034] The above hydrophobic liquid crystal may be a liquid crystal selected from the group consisting of nematic, smectic, and cholesteric liquid crystals, and any hydrophobic liquid crystal having a liquid crystal phase within a specific temperature range may be used without limitation.

[0035] Specifically, as illustrated in FIG. 1, in the embodiments of the present invention, the hydrophobic liquid crystal may be a mixture of cholesteryl oleyl carbonate, cholesteryl nonanoate, and cholesteryl chloride, which are substances approved by the Ministry of Food and Drug Safety, and may be used without limitation as long as the mixing ratio maintains the liquid crystal phase range.

[0036] In addition, the surfactant of FIG. 1 may be composed of glyceryl stearate, stearic acid, lauric acid, myristic acid, palmitic acid, polyglyceryl-10 stearate, polyglyceryl-6 stearate, polyglyceryl-10 laurate, glyceryl monostearate, etc.

[0037] The method for preparing the oil phase component of the hydrophobic liquid crystal-based ceramide carrier of Fig. 1 involves adding hydrophobic liquid crystal : ceramide : surfactant in a weight ratio (wt%) of 100~50 (excluding 100) : 0~25 (excluding 0) : 0~25 (excluding 0), heating the mixture to 120°C, and mixing it uniformly with a stirrer. Here, this ratio is the ratio that allows for the maximum loading of ceramide while maintaining hydrophobic liquid crystal properties. If more ceramide and surfactant are added than this ratio, there is a problem in that the liquid crystal phase is not exhibited at room temperature, and thus the liquid crystal properties are not displayed.

[0038] In Figure 1, if the ceramide is loaded at a ratio of more than 10 wt% relative to the total mixture, it is harmful to the human body; therefore, to ensure that the weight ratio of ceramide is less than 10 wt% relative to the total mixture, the above oil phase component is added to water and heated at 160°C for 30 minutes. Subsequently, to increase the stability of the hydrophobic liquid crystal-based ceramide carrier, a thickener and a neutralizing agent are added in a weight ratio of mixture : thickener (1% carbomer) : neutralizing agent (10% triethylamine) = 84.6 : 14 : 1.4, and then heated at 160°C for 30 minutes. Afterward, to form microdroplets of uniform size, the mixture is mixed at 3000 rpm for 90 seconds using a vortex mixer. At this time, microdroplets can also be formed using a homomixer, a high-pressure emulsifier, or an ultrasonic disperser.

[0039] Figure 2 shows photographs of hydrophobic liquid crystal-based ceramide carriers observed through a polarizing microscope, where (a) is a photograph of a liquid crystal droplet without ceramide and (b) is a photograph of a hydrophobic liquid crystal-based ceramide carrier.

[0040] Specifically, through the change in polarized microscope images from Fig. 2(a) to Fig. 2(b), it was confirmed that the orientation of hydrophobic liquid crystal molecules changed from a horizontal orientation (Fig. 2(a)) to a vertical orientation (Fig. 2(b)) due to the self-assembly of ceramide.

[0041] Figure 3 shows the self-assembly characteristics of the ceramide surface of hydrophobic liquid crystals and hydrophobic oils, and compares the amount of ceramide loaded on the ceramide surface of hydrophobic liquid crystal-based ceramide carriers with the actual amount loaded.

[0042] Figure 3a) shows the self-assembly characteristics at the water interface of mineral oil, a representative hydrophobic oil, and E7, a representative hydrophobic liquid crystal, mixed with ceramide and a surfactant.

[0043] Referring to Fig. 3a), the hydrophobic tails of the amphiphilic material self-assembled at the hydrophobic liquid crystal interface are aligned perpendicular to the interface and stabilized. Therefore, the effective volume occupied by a single amphiphilic molecule is smaller compared to a typical hydrophobic and hydrophilic interface. In mineral oil, the interface becomes saturated at a weight ratio of ceramide to surfactant of 0.25 wt%, and the interfacial tension is maintained at subsequent concentrations; however, in E7, the interface becomes saturated at a weight ratio of ceramide to surfactant of 3 wt%. Thus, it was confirmed that the effective volume occupied by a single amphiphilic molecule at the interface of the hydrophobic liquid crystal is smaller than that of hydrophobic oil. Due to this effect, the hydrophobic liquid crystal carrier of the present invention can carry more ceramide molecules.

[0044] Figure 3b) shows a comparison between the amount of ceramide self-assembled on the surface and the amount of ceramide loaded onto the actual hydrophobic liquid crystal-based ceramide carrier.

[0045] The amount of ceramide self-assembled on the surface, represented by the black line in Fig. 3b), was calculated as follows. After fabricating a hydrophobic liquid crystal-based ceramide carrier, the surface area of ​​the liquid crystal carrier was calculated through polarized light microscope image analysis, and the amount of ceramide theoretically self-assembled on the surface was calculated by dividing it by the surface effective volume of ceramide known in previous papers.

[0046] Referring to Fig. 3b), the actual amount of ceramide loaded is greater than the surface load calculated. This confirms the possibility that ceramide is also loaded inside the carrier.

[0047] Figure 4 shows the change in the phase transition temperature of the oil phase component and the change in brightness of the polarized microscope image as evidence of the formation of a structure inside a hydrophobic liquid crystal-based ceramide carrier.

[0048] Figure 4a) shows the change in phase transition temperature and the change in brightness of the polarized light microscope image when the concentration of ceramide and surfactant is increased in the hydrophobic liquid crystal.

[0049] Referring to Fig. 4a), it was confirmed that ceramide and surfactant do not dissolve in the liquid crystal, as there is no change in the phase transition temperature of the hydrophobic liquid crystal up to a weight ratio of 0.4 wt% of ceramide and surfactant. Above a weight ratio of 0.4 wt%, a decrease in the phase transition temperature and an increase in the brightness of the polarized light microscope image are observed, which is evidence that a structure has been formed inside the liquid crystal, as is known from previous research results.

[0050] Figures 4b, c), and d) are polarized light microscope images of a mixture of hydrophobic liquid crystal, ceramide, and surfactant, which are the oil phase components. The weight ratio shown in the figure represents the weight ratio of (ceramide + surfactant) to the hydrophobic liquid crystal. In this case, the mixture was added at a weight ratio of ceramide : surfactant = 1 : 1. Figure 4b) shows an oily streak structure observed when the cholesteric liquid crystal has a parallel orientation on the surface. Figure 4c) shows a focal conic structure observed when the arrangement of the cholesteric liquid crystal becomes non-uniform compared to Figure 4b due to internal structures. Figure 4d) shows a structure with very small birefringence when the arrangement of the cholesteric liquid crystal becomes non-uniform due to multiple internal structures, leaving only local liquid crystal arrangements.

[0051] It was confirmed through Figure 4 that a structure was formed inside the hydrophobic liquid crystal.

[0053] [Experimental Example]

[0054] Example 1: Preparation of a hydrophobic liquid crystal-based ceramide carrier.

[0055] Hydrophobic liquid crystal (cholesteryl oleyl carbonate: cholesteryl nonanoate, cholesteryl chloride = 33:22:45), ceramide, and surfactant (glyceryl stearate: stearic acid = 50:50) are mixed in a weight ratio (hydrophobic liquid crystal: ceramide: surfactant = 50:25:25), heated at 120°C for 30 minutes, and uniformly mixed using a stirrer. At this time, the ceramide can be uniformly dispersed in the liquid crystal due to the hydrophobic properties of the liquid crystal. The above ratio is the ratio that allows the maximum loading of ceramide while maintaining the hydrophobic properties of the liquid crystal.

[0056] Subsequently, the above mixture was added to water and heated at 160°C for 30 minutes; then, 1% carbomer and 10% triethylamine were added, and the mixture was heated at 160°C for 30 minutes. Here, the ratio of each substance was mixed as follows: (Water : Cholesteryl oleyl carbonate : Cholesteryl nonanoate : Cholesteryl chloride : Ceramide : Glyceryl styrene : Stearic acid : 1% carbomer : 10% triethylamine = 44.6 : 6.6 : 4.4 : 9 : 10 : 5 : 5 : 14 : 1.4). Afterward, to form microdroplets of uniform size, the mixture was mixed using a vortex mixer at 3,000 rpm for 90 seconds. At this time, microdroplet formation is also possible using a homomixer, a high-pressure emulsifier, or an ultrasonic disperser. (Example 1)

[0057] Cholesteryl oleyl carbonate: USA, Sigma-Aldrich

[0058] Cholesteryl Nonanoate: USA, Sigma-Aldrich

[0059] Cholesteryl chloride: USA, Sigma-Aldrich

[0060] Ceramide: Ceramide 3 (NP), South Korea, Solus Advanced Materials

[0061] Glyceryl Stearate: UK, Croda

[0062] Stearic acid: USA, OLEOCHEMICALS

[0063] Carbomer: Carbopol 981, USA, Lubrizol

[0064] Triethylamine: USA, Dow Chemical

[0066] <Experimental Example 1> Confocal Fluorescence Microscopy Analysis

[0067] In order to confirm the internal detection characteristics of the ceramide of the hydrophobic liquid crystal-based ceramide carrier according to an embodiment of the present invention, the internal structure of the hydrophobic liquid crystal-based ceramide carrier formed through Example 1 was photographed using a confocal fluorescence microscope, and the results are shown in FIG. 5b).

[0068] FIGS. 5a) and FIGS. 5b) are diagrams illustrating that ceramide is supported inside a hydrophobic liquid crystal-based ceramide carrier.

[0069] Figure 5a) is a photograph taken when the weight ratio of ceramide to the total mixture is 0 wt% and only hydrophobic liquid crystals are dispersed in water, confirming that the fluorescence brightness inside the carrier is uniform.

[0070] Figure 5b) is an internal photograph of a hydrophobic liquid crystal-based ceramide carrier formed through Example 1, in which the weight ratio of ceramide to the total mixture is 10 wt%. The fluorescence brightness inside is not uniform, and through comparison with Figure 5a), it was confirmed that ceramide is loaded in the darker fluorescence brightness areas.

[0072] <Experimental Example 2> Wide-angle X-ray Scattering (WAXS) Analysis

[0073] In order to confirm the internal loading and structure of the hydrophobic liquid crystal-based ceramide carrier according to an embodiment of the present invention, the internal structure of the hydrophobic liquid crystal-based ceramide carrier formed through Example 1 was analyzed by wide-angle X-ray scattering, and the results are shown in Fig. 6a).

[0074] Figure 6a) shows the wide-angle X-ray scattering analysis results for Example 1, from bottom to top, ceramide, hydrophobic liquid crystal, surfactant, the case without liquid crystal in Example 1 (when a mixture of ceramide and surfactant is dispersed in water).

[0075] FIG. 6b) is a polarized microscope image of a hydrophobic liquid crystal dispersed in water, FIG. 6c) is a polarized microscope image of the case without liquid crystal in Example 1, and FIG. 6d) is a polarized microscope image of Example 1.

[0076] Referring to Fig. 6a), in the hydrophobic liquid crystal-based ceramide carrier of Example 1, peaks corresponding to the hydrophobic liquid crystal and (mixed structure of ceramide and surfactant) are observed, while peaks corresponding to ceramide and surfactant are not observed. Therefore, it was confirmed that the hydrophobic liquid crystal-based ceramide carrier is composed of the hydrophobic liquid crystal and (mixed structure of ceramide and surfactant).

[0077] Referring to Figs. 6b), c), and d), it was confirmed that the hydrophobic liquid crystal-based ceramide carrier of Example 1 in Fig. 6d) exhibits almost no birefringence, unlike Figs. 6b) and c). Through Fig. 6a), it was confirmed that the structures of Figs. 6b) and c) should exist in Fig. 6d), but since no carriers with strong birefringence were observed in Fig. 6d), the structures of Figs. 6b) and c) were formed within Fig. 6d).

[0079] [Characteristics Evaluation]

[0080] The hydrophobic liquid crystal-based ceramide carrier was confirmed to have 1) high dispersibility, 2) high ceramide loading capacity, and 3) high stability.

[0081] 1) High dispersibility: It was confirmed through a polarizing microscope that despite the large number of hydrophobic liquid crystal-based ceramide carriers, the carriers did not combine with each other (Fig. 7(a)).

[0082] 2) High ceramide loading capacity: As shown in Fig. 7(b), no phase separation or crystallization is observed even when 10 wt% of ceramide is added.

[0083] 3) High stability: It was confirmed that the size of the hydrophobic liquid crystal carriers did not change even after 17 days, and that no phase separation or crystallization occurred in the vial. (Fig. 8)

[0084] In this invention, a manufacturing technology for a ceramide carrier using hydrophobic liquid crystals was developed to solve the problems of existing hydrophilic liquid crystal-based ceramide carriers. Through this, high dispersibility and stability were achieved, and a higher ceramide loading rate than existing carriers was attained.

[0086] The above detailed description is illustrative of the present invention. Furthermore, the foregoing describes preferred embodiments of the present invention, and the present invention may be used in various other combinations, modifications, and environments. That is, modifications or alterations are possible within the scope of the concept of the invention disclosed herein, the scope equivalent to the disclosed content, and / or the scope of the art or knowledge. The described embodiments describe the best state for implementing the technical concept of the present invention, and various modifications required for specific fields of application and uses of the present invention are possible. Accordingly, the above detailed description of the invention is not intended to limit the present invention to the disclosed embodiments. Additionally, the appended claims should be interpreted as including other embodiments.

Claims

Claim 1 A) a step of uniformly mixing a hydrophobic liquid crystal, a ceramide, and a surfactant to produce a mixture; and B) a step of dispersing the mixture in water to form liquid crystal microdroplets; and C) a step of self-assembling the ceramide at the droplet interface and supporting it inside the droplets; wherein, in step A), the mixing ratio of the hydrophobic liquid crystal, the ceramide, and the surfactant is 50:25:25 by weight, and the mixing ratio of the hydrophobic liquid crystals cholesteryl oleyl carbonate, cholesteryl nonanoate, and cholesteryl chloride is 33:22:45 by weight. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 A method for preparing a ceramide carrier using a hydrophobic liquid crystal, wherein the surfactant comprises at least one of lauric acid, myristic acid, palmitic acid, stearic acid, polyglyceryl-10 stearate, polyglyceryl-6 stearate, polyglyceryl-10 laurate, and glyceryl monostearate. Claim 6 A method for manufacturing a ceramide carrier using a hydrophobic liquid crystal in which the mixing ratio of glyceryl stearate and stearic acid is 50:50 by weight, wherein the surfactant is a ceramide carrier. Claim 7 A hydrophobic liquid crystal-based ceramide carrier comprising liquid crystal microdroplets formed by dispersing a mixture containing a hydrophobic liquid crystal, ceramide, and a surfactant in water, wherein some of the ceramide self-assembles at the droplet interface and some is supported inside the droplet, wherein the mixing ratio of the hydrophobic liquid crystal, ceramide, and surfactant in the mixture is 50:25:25 by weight, and the mixing ratio of cholesteryl oleyl carbonate, cholesteryl nonanoate, and cholesteryl chloride in the hydrophobic liquid crystal is 33:22:45 by weight. Claim 8 delete Claim 9 delete Claim 10 delete Claim 11 In claim 7, the surfactant is a hydrophobic liquid crystal-based ceramide carrier comprising at least one of lauric acid, myristic acid, palmitic acid, stearic acid, polyglyceryl-10 stearate, polyglyceryl-6 stearate, polyglyceryl-10 laurate, and glyceryl monostearate. Claim 12 In claim 11, the surfactant is a hydrophobic liquid crystal-based ceramide carrier in which the mixing ratio of glyceryl stearate and stearic acid is 50:50 by weight.

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