Inner beauty composition containing low molecular weight collagen for skin improvement and method of manufacture thereof

Abstract

The present invention relates to an inner beauty composition for skin improvement containing low molecular weight collagen comprising purified water, low molecular weight collagen, prolastin, vitamin C, hyaluronic acid, enzyme-treated stevia, vitamin mineral mix, milk ceramide, biotin, glutathione yeast extract, and hydrated citric acid.

Description

Technology Field

[0001] The present invention relates to an inner beauty composition for skin improvement containing low molecular weight collagen and a method for preparing the same. More specifically, it relates to an inner beauty composition containing low molecular weight collagen and ingredients having a skin improvement effect, which has an excellent skin improvement effect, and a method for preparing the same. Background Technology

[0002] Collagen is a fibrous protein that constitutes most of the connective tissues in the body, including animal skin, bones, fascia, cartilage, and hair. The raw materials for this collagen can be classified into animal collagen obtained from animals such as cows and pigs, fish collagen obtained from fish, and plant collagen extracted from plants.

[0003] Animal collagen is the most readily available raw material, but it has the disadvantage of low absorption rate in the body due to its large molecular weight, while plant collagen has the disadvantage of lacking hydroxyproline, a basic component of collagen.

[0004] Meanwhile, fish collagen obtained from fish can be produced as low-molecular-weight collagen by enzymatically breaking down fish scales, and it has the advantage of high absorption rate in the body due to its very small molecular size.

[0005] As low molecular weight collagen is effective in helping to moisturize and improve skin elasticity, the number of people applying collagen-containing cosmetics to their skin or consuming collagen supplements in the form of pills to aid in its absorption into the body is steadily increasing.

[0006] However, collagen has the disadvantage that the amount absorbed into the skin is extremely small even when applied topically because it is difficult to absorb through the skin. Additionally, caution is required with collagen supplements in pill form, as some collagen may be broken down during digestion, potentially reducing absorption rates and causing side effects such as heartburn in people with weak digestive systems.

[0007] Although collagen has the highest absorption rate in the body when consumed in liquid form, it is broken down as it passes through various digestive organs. Therefore, it is advisable to consume it together with other ingredients such as vitamin C and glutathione, which can inhibit the breakdown of collagen while reducing side effects such as heartburn.

[0008] While the aforementioned other ingredients can exhibit complementary effects when consumed simultaneously with collagen, issues regarding taste and aroma may arise depending on the ratio of ingredients, and side effects such as gastrointestinal disorders and reduced absorption rates may occur. Therefore, the appropriate combination of each ingredient is currently required.

[0009] Against this backdrop, the inventors developed an inner beauty composition and a method for manufacturing the same that improves the body's absorption rate of collagen while having excellent taste and aroma and superior skin improvement effects, and completed the present invention by confirming the effects. Prior art literature

[0010] Republic of Korea Published Patent Application No. 10-2024-0082827 (Published June 11, 2024) The problem to be solved

[0011] The present invention was created to solve the aforementioned conventional problems, and aims to provide an inner beauty composition and a method for manufacturing the same that improves the absorption rate of collagen in the body while having excellent taste and aroma and superior skin improvement effects.

[0012] The technical problems of the present invention are not limited to those mentioned above, and other unmentioned technical problems will be clearly understood by a person skilled in the art from the description below. means of solving the problem

[0013] The above objective is achieved by an inner beauty composition for skin improvement containing low molecular weight collagen comprising purified water, low molecular weight collagen, prolastin, vitamin C, hyaluronic acid, enzyme-treated stevia, vitamin mineral mix, milk ceramide, biotin, glutathione yeast extract, and hydrated citric acid.

[0014] In addition, it may include 11,000 to 12,000 parts by weight of purified water, 4,950 to 5,050 parts by weight of low molecular weight collagen, 90 to 110 parts by weight of prolastin, 150 to 250 parts by weight of vitamin C, 110 to 130 parts by weight of hyaluronic acid, 60 to 70 parts by weight of enzyme-treated stevia, 1 to 3 parts by weight of vitamin mineral mix, 1 to 3 parts by weight of milk ceramide, 0.01 to 0.03 parts by weight of biotin, 0.1 to 0.3 parts by weight of glutathione yeast extract, and 50 to 60 parts by weight of hydrated citric acid.

[0015] In addition, it may further include lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract, fructooligosaccharide, and natural lemon-lime flavor.

[0016] In addition, it may include 950 to 1,050 parts by weight of lemon concentrate, 250 to 350 parts by weight of lime concentrate, 350 to 450 parts by weight of agave syrup, 3 to 5 parts by weight of grapefruit seed extract, 950 to 1,050 parts by weight of fructooligosaccharide, and 115 to 135 parts by weight of natural lemon-lime flavor.

[0017] The above objective is achieved by: a first mixing step of mixing purified water, low molecular weight collagen, prolastin, vitamin C, hyaluronic acid, enzyme-treated stevia, vitamin mineral mix, milk ceramide, biotin, glutathione yeast extract, and hydrated citric acid to produce a first mixture; a first stirring step of stirring the first mixture at 60 to 70 degrees for 10 minutes; a second mixing step of adding lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract, and fructooligosaccharide to the stirred first mixture to produce a second mixture; a second stirring step of stirring the second mixture at 60 to 70 degrees for 15 minutes; and a third mixing step of adding natural lemon-lime flavor to the stirred second mixture to produce a third mixture. This is achieved by a method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen, comprising a third stirring step of stirring the above third mixture at 60 to 70 degrees for 10 minutes.

[0018] In addition, the third mixture may have a sugar content of 37 to 47 brix and a hydrogen ion concentration index (PH) of 3.9 to 4.9. Effects of the invention

[0019] According to an embodiment of the present invention, an inner beauty composition for skin improvement containing low molecular weight collagen and a method for preparing the same can inhibit collagen degradation so that the collagen absorption rate can be maximized, and an inner beauty composition with excellent skin improvement effects such as skin elasticity and moisturization can be provided.

[0020] In addition, it has the effect of providing an inner beauty composition that is easy to consume due to its excellent taste and aroma. Specific details for implementing the invention

[0021] The configurations of the embodiments described in this specification are merely preferred examples of the disclosed invention, and various modifications that may replace the embodiments of this specification may exist at the time of filing this application.

[0022] The terms used herein are for describing embodiments and are not intended to limit or / or restrict the disclosed invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, terms such as “comprising” or “having” are intended to indicate the presence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and do not preclude the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0024] Terms including ordinal numbers, such as "first," "second," etc., as used in this specification may be used to describe various components, but said components are not limited by said terms, and said terms are used solely for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be named the second component, and similarly, the second component may be named the first component.

[0025] Furthermore, in describing the present invention, if it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the essence of the invention, such detailed description is abbreviated or omitted.

[0027] From now on, an inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention will be described in detail.

[0028] An inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention comprises purified water, low molecular weight collagen, prolastin, vitamin C, hyaluronic acid, enzyme-treated stevia, vitamin mineral mix, milk ceramide, biotin, glutathione yeast extract, and hydrated citric acid.

[0029] The skin-improving inner beauty composition containing such low molecular weight collagen can improve the absorption rate of collagen in the body by including low molecular weight collagen, and can improve the collagen synthesis rate and the absorption rate of collagen in the body by including vitamin C, biotin, glutathione, and yeast extract, and can maximize skin improvement effects such as skin moisturization and skin elasticity along with collagen by including prolastin, hyaluronic acid, and milk ceramide.

[0030] To explain in more detail, an inner beauty composition for skin improvement containing low molecular weight collagen may comprise 11,000 to 12,000 parts by weight of purified water, 4,950 to 5,050 parts by weight of low molecular weight collagen, 90 to 110 parts by weight of prolastin, 150 to 250 parts by weight of vitamin C, 110 to 130 parts by weight of hyaluronic acid, 60 to 70 parts by weight of enzyme-treated stevia, 1 to 3 parts by weight of vitamin mineral mix, 1 to 3 parts by weight of milk ceramide, 0.01 to 0.03 parts by weight of biotin, 0.1 to 0.3 parts by weight of glutathione yeast extract, and 50 to 60 parts by weight of hydrated citric acid.

[0031] The combination of each ingredient described above is an optimal ratio derived through experiments to produce the best effect in terms of efficacy and to minimize the occurrence of side effects caused by each ingredient.

[0033] In the present invention, purified water refers to water from which all impurities, such as dissolved ions, solid particles, microorganisms, organic matter, and dissolved gases contained in the water, have been removed. The purified water is mixed with each component to enable the composition to be formed in a liquid form.

[0034] In the present invention, low molecular weight collagen refers to collagen with a small molecular size. Collagen with a molecular size of 5,000 Da or more is classified as high molecular weight collagen, and collagen with a molecular size of less than 5,000 Da can be classified as low molecular weight collagen. Because low molecular weight collagen has a small molecular size, its absorption rate in the body can be significantly increased.

[0035] The low molecular weight collagen of the present invention is preferably fish collagen with a molecular weight of less than 5,000 Da. Fish collagen is collagen extracted from the skin and scales of fish and has the advantage of having a higher absorption rate compared to animal and plant collagens and a significantly lower potential for causing allergies. Furthermore, because it has a higher absorption rate in the body compared to animal and plant collagens, it is effective in promoting skin, joint, and bone health. While the elasticity and moisturizing effects of the skin are maximized as the intake of low molecular weight fish collagen increases, caution is required as excessive intake may cause indigestion and gastrointestinal disorders.

[0036] The prolastin in the present invention may be an elastin hydrolysate. Prolastin is obtained by breaking down elastin protein into peptides through enzymatic or chemical methods, and has the advantage of a higher absorption rate in the body than general elastin protein. When absorbed into the body, prolastin is effective for skin elasticity and hydration, and has the effect of improving wrinkles. Both prolastin and collagen are important proteins for maintaining the structure and function of the skin; they play complementary roles and can contribute to maintaining skin elasticity and strength. Collagen increases skin strength and durability, while prolastin increases skin flexibility and elasticity; thus, the effects of skin elasticity, hydration, and wrinkle prevention can be further enhanced compared to when absorbed into the body as a single component.

[0037] Vitamin C in the present invention may be in powder form. However, the form of vitamin C is not limited to powder, and it may be implemented as a liquid as long as it is in a form that can be dissolved in purified water. Vitamin C acts as an important factor in collagen synthesis and can maximize the effect of collagen synthesis by influencing the hydrogen bonds of proline and hydroxyproline, which maintain the structure of collagen. While the effect of collagen synthesis can be maximized when 200 mg or more of vitamin C is used, consuming more than 250 mg may cause excessive stomach acid, leading to gastrointestinal disorders such as heartburn; therefore, an appropriate amount of intake is required.

[0038] The hyaluronic acid of the present invention is a disaccharide polymer of glucuronic acid and N-acetylglucosamine, capable of absorbing 100 to 10,000 times its own weight in water. This hyaluronic acid is absorbed into the body and retains moisture, thereby keeping the skin hydrated and preventing it from drying out. In addition, hyaluronic acid has the effect of promoting the wound healing process and reducing inflammation. While this hyaluronic acid shows good effects on skin elasticity as the intake increases, excessive intake may cause side effects such as internal water imbalance and edema, and may induce skin troubles or itching. Therefore, intake in an appropriate amount is required.

[0039] The enzyme-treated stevia of the present invention is a general stevia extract that has been treated with enzymes to convert or increase specific components. Compared to general stevia, it has no bitter taste and has an even greater sweetness, so it has the effect of producing sweetness with a small amount.

[0040] The vitamin and mineral mix of the present invention is a mixture for simultaneously supplying vitamins and minerals, wherein the vitamins include one or more of vitamin A, B vitamins, vitamin C, and vitamin E, and the minerals may include one or more of zinc, iron, calcium, and sodium.

[0041] The milk ceramide of the present invention refers to ceramide extracted from milk. Milk ceramide can strengthen the protective barrier of the skin's epidermal layer, retain moisture, and protect the skin from external stimuli. As a natural ceramide extracted from milk, milk ceramide is an animal-derived ceramide that has the advantage of a higher absorption rate in the body compared to plant-derived ceramides. When this milk ceramide is taken together with collagen, which constitutes the dermis, effects such as elasticity and hydration can occur throughout the skin from the epidermis to the dermis; thus, skin improvement effects, such as elasticity and hydration, can be further enhanced compared to when absorbed into the body as a single substance.

[0042] The biotin of the present invention is one of the vitamin B complex and can reduce skin inflammation and promote skin regeneration upon absorption into the body. Furthermore, since biotin can improve the collagen synthesis rate by participating in the generation of skin cells, consuming biotin and collagen together can improve the collagen synthesis rate by more than twofold.

[0043] The glutathione yeast extract of the present invention refers to a component extracted from yeast rich in glutathione. The glutathione yeast extract is a powerful antioxidant that can play an important role in protecting cells by neutralizing free radicals and supporting immune function. Furthermore, it can even out skin tone by regulating melanin production. This glutathione yeast extract can inhibit the breakdown of collagen by reducing collagen damage through its antioxidant action within the body.

[0044] The hydrated citric acid of the present invention is citric acid containing water and can be implemented mainly in powder form. Hydrated citric acid has a sour taste and is widely used as an acidulant in the food industry; it can combine with minerals such as iron and calcium to promote the absorption of minerals in the body. Furthermore, hydrated citric acid can control the acidity of the composition of the present invention.

[0046] An inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention may further include lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract, fructooligosaccharide, and natural lemon-lime scent.

[0047] An inner beauty composition for skin improvement containing low molecular weight collagen with additional ingredients as described above may be made easier for the user to consume by adding a refreshing and sweet taste.

[0048] To explain in more detail, an inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention may comprise 950 to 1,050 parts by weight of lemon concentrate, 250 to 350 parts by weight of lime concentrate, 350 to 450 parts by weight of agave syrup, 3 to 5 parts by weight of grapefruit seed extract, 950 to 1,050 parts by weight of fructooligosaccharide, and 115 to 135 parts by weight of natural lemon-lime scent.

[0049] The combination of each ingredient described above is the optimal ratio derived through experiments to ensure the best taste and ease of consumption.

[0051] In addition, the inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention may include solid sugars. The solid sugar may be sugar, isomaltose, deco sugar (Hagel sugar), unrefined raw sugar, etc., or liquid fructose or tremoline concentrated and solidified, or deco snow or sugar powder compressed to a large particle size and solidified. For the texture of the inner beauty composition, the size of the solid sugar is suitable to be 0.5 mm to 5 mm, preferably 1 mm to 3 mm.

[0053] The lemon concentrate of the present invention refers to a liquid made in a concentrated form by removing water from an extract obtained by squeezing or pressing lemons. It can produce a sour taste, is rich in Vitamin C, and has antioxidant effects, which can help maintain skin health and elasticity. Here, it is preferable that the lemon concentrate has a sugar content between 60 and 70 Brix. This is because if the sugar content is less than 60 Brix, a sour and bitter taste may be perceived, and if it exceeds 70 Brix, the sweet taste is perceived more strongly than the sour taste, making it impossible to provide a refreshing taste.

[0054] The lime concentrate of the present invention refers to a liquid produced in a concentrated form by removing water from an extract obtained by squeezing or pressing limes. The lime concentrate is rich in vitamins and has antioxidant effects, which can help strengthen skin health and the immune system.

[0055] The agave syrup of the present invention refers to a natural sugar with a low glycemic index, extracted from the piña, the base part of the agave, a type of cactus. Unlike artificial sweeteners, agave syrup is a healthy natural sugar with guaranteed stability, and it is a sugar that is good to consume instead of sugar because it has effects such as lowering lipids and blood cholesterol and reducing weight.

[0056] The grapefruit seed extract of the present invention is a substance extracted from grapefruit seeds and has antibacterial, antioxidant, and anti-inflammatory effects, so it can be utilized in various products. Thanks to its anti-inflammatory effect, it can help manage skin conditions such as acne, eczema, and psoriasis, and it is rich in antioxidants such as flavonoids and vitamin C, which can help remove free radicals in the body and prevent cell damage.

[0057] The fructooligosaccharide of the present invention is a natural sugar, meaning an oligosaccharide in which one or more fructose molecules are added in a chain to sugar. As a type of prebiotic, it promotes the growth of beneficial bacteria in the intestines to improve intestinal health, and has the advantage of providing sweetness without causing a rapid rise in blood sugar levels because it is digested slowly in the body.

[0058] The natural lemon-lime scent of the present invention refers to a fragrance containing the scent of lemon and lime extracted from natural ingredients. It is used in food, beverages, etc., and is characterized by a refreshing and fresh scent. Because it is a natural ingredient, it has the advantage of posing a lower risk of allergies or skin irritation compared to artificial fragrances.

[0060] Hereinafter, a method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention will be described.

[0061] A method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen comprises: a first mixing step of mixing purified water, low molecular weight collagen, prolastin, vitamin C, hyaluronic acid, enzyme-treated stevia, vitamin mineral mix, milk ceramide, biotin, glutathione yeast extract, and hydrated citric acid to produce a first mixture; a first stirring step of stirring the first mixture at 60 to 70 degrees for 10 minutes; a second mixing step of adding lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract, and fructooligosaccharide to the stirred first mixture to produce a second mixture; a second stirring step of stirring the second mixture at 60 to 70 degrees for 15 minutes; a third mixing step of adding natural lemon-lime scent to the stirred second mixture to produce a third mixture; and a third stirring step of stirring the third mixture at 60 to 70 degrees for 10 minutes.

[0063] The first mixing step is a step of producing a first mixture by mixing purified water, low molecular weight collagen, prolastin, vitamin C, hyaluronic acid, enzyme-treated stevia, vitamin mineral mix, milk ceramide, biotin, glutathione yeast extract, and hydrated citric acid.

[0064] To explain in more detail, in the first step, 11,000 to 12,000 parts by weight of purified water, 4,950 to 5,050 parts by weight of low molecular weight collagen, 90 to 110 parts by weight of prolastin, 150 to 250 parts by weight of vitamin C, 110 to 130 parts by weight of hyaluronic acid, 60 to 70 parts by weight of enzyme-treated stevia, 1 to 3 parts by weight of vitamin mineral mix, 1 to 3 parts by weight of milk ceramide, 0.01 to 0.03 parts by weight of biotin, 0.1 to 0.3 parts by weight of glutathione yeast extract, and 50 to 60 parts by weight of hydrated citric acid may be mixed.

[0065] In the first mixing step, each component can be weighed in the aforementioned weight portions and each introduced into the hopper mixer. The introduced components can be mixed together to produce the first mixture.

[0066] Here, each component can be introduced in either liquid or powder form.

[0067] The first stirring step is a step of stirring the first mixture at 60 to 70 degrees for 10 minutes.

[0068] Here, the temperature refers to the temperature of the hopper mixer, and it is preferable that the hopper mixer temperature be 65 degrees. This is because if the hopper mixer temperature is below 60 degrees, collagen and other components may not completely dissolve in purified water, resulting in an unhomogenized mixture that may form lumps, and increasing the likelihood of some pathogenic microorganisms or bacteria surviving and multiplying. Conversely, if the hopper mixer temperature exceeds 70 degrees, denaturation of the collagen may occur, leading to a decrease in its function, and nutrients in heat-sensitive components such as Vitamin C and vitamin-mineral mix may be destroyed. Therefore, it is preferable that the hopper mixer temperature be 65 degrees so that collagen and other components can be completely dissolved in purified water without being destroyed, and so that the proliferation of microorganisms and bacteria can be inhibited.

[0069] The second mixing step is a step of producing a second mixture by adding lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract, and fructooligosaccharide to the stirred first mixture.

[0070] To explain in more detail, the second mixing step may involve mixing 950 to 1,050 parts by weight of lemon concentrate, 250 to 350 parts by weight of lime concentrate, 350 to 450 parts by weight of agave syrup, 3 to 5 parts by weight of grapefruit seed extract, and 950 to 1,050 parts by weight of fructooligosaccharide into the stirred first mixture.

[0071] In the second mixing step, each component may be weighed in the aforementioned weight portions and introduced into the hopper mixer where the stirred first mixture is located. The introduced components may be mixed together to produce the second mixture. Here, each component may be introduced in either a liquid or a powder form.

[0072] The second stirring step is a step of stirring the second mixture at 60 to 70 degrees for 15 minutes. At this time, it is preferable that the temperature of the hopper mixer be 65 degrees, the same as in the first stirring step. The stirring time of the second stirring step is characterized by being longer than that of the first stirring step. This is because the second mixture stirred in the second stirring step contains more components than the first mixture, and the lemon concentrate, lime concentrate, agave syrup, and fructooligosaccharide mixed in the second mixing step are components that determine the taste of the composition, so it is important that they are completely dissolved and uniformly distributed.

[0073] The third mixing step is a step of creating a third mixture by adding natural lemon-lime scent to the stirred second mixture. More specifically, the third mixing step may further add 115 to 135 parts by weight of natural lemon-lime scent to the stirred second mixture.

[0074] In the third mixing step, the natural lemon-lime scent may be weighed in the aforementioned weight portion and introduced into the hopper mixer where the second mixture is located. Each introduced component may be mixed with one another to produce the third mixture. Here, the natural lemon-lime scent may be introduced in either liquid or powder form.

[0075] The third stirring step is a step of stirring the third mixture at 60 to 70 degrees for 10 minutes. At this time, it is preferable that the temperature of the hopper mixer be 65 degrees, the same as in the first and second stirring steps, and stirring can be done for 10 minutes. This is because if the natural lemon-lime scent is stirred for less than 10 minutes, the scent is not sufficiently dispersed, and if it is stirred for more than 10 minutes, volatilization of the scent may occur and the scent may be lost.

[0076] Meanwhile, the third mixture that has undergone the third stirring step may be a liquid composition having a sugar content of 37 to 47 Birx and a hydrogen ion concentration index of 3.9 to 4.9.

[0077] More preferably, the third mixture obtained from the third stirring step may be a liquid composition with 42 Brix and pH 4.4. 42 Brix signifies a very high sugar content and may have a thick texture similar to thin syrup. pH 4.4 signifies a weak acidity and can neutralize sweetness and provide acidity upon consumption. This high sugar content and acidity can neutralize the fishy taste produced by low molecular weight fish collagen.

[0078] A method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention may further include a heat treatment step of adding solid sugars, placing them in a molding mold, and performing heat treatment.

[0079] The heat treatment step includes the step of adding solid sugar having the aforementioned components, and for the texture, the size of the solid sugar is suitable to be 0.5 mm to 5 mm, preferably 1 mm to 3 mm.

[0080] The heat treatment step may include the step of introducing solid sugars and then placing the material into a molding mold. When the solid sugars are placed in the molding mold with the molding material distributed on the bottom surface and slight pressure is applied, the pre-distributed solid sugar particles become embedded in the bottom surface, and a certain portion of the bottom surface of the dough is filled with solid sugars. With the molding mold filled with the composition containing solid sugars, the molding mold is placed in an oven to perform heat treatment. As heat treatment proceeds, the composition begins to cook first and solidifies starting from the surface. Subsequently, as the heat treatment continues, the solid sugars turn into a liquid state and escape from the portion of the composition they originally occupied, creating empty spaces that will provide surface pores, and the composition becomes fully cooked.

[0081] A method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention can provide an improved texture through a heat treatment step.

[0083] The composition produced by the method for manufacturing an inner beauty composition for skin improvement containing low molecular weight collagen described above is in a liquid form containing low molecular weight collagen, has a high absorption rate of collagen in the body, and contains ingredients that help inhibit collagen breakdown and promote collagen production, thereby enhancing the efficacy of collagen.

[0084] Furthermore, it contains ingredients that improve skin and provide antioxidant effects, so the skin improvement effect can be excellent.

[0085] In addition, a composition can be provided in which the skin improvement effect is increased by the mixing ratio of each ingredient, while side effects caused by the ingredients are minimized and the calorie content is not high.

[0087] Example_ A first composition prepared by a manufacturing method according to one embodiment of the present invention

[0088] According to the manufacturing method of one embodiment of the present invention, 11627.8 mg of purified water, 5000 mg of low molecular weight collagen, 100 mg of prolastin, 200 mg of vitamin C, 120 mg of hyaluronic acid, 64 mg of enzyme-treated stevia, 2 mg of vitamin mineral mix, 2 mg of milk ceramide, 0.02 mg of biotin, 0.2 mg of glutathione yeast extract, and 56 mg of hydrated citric acid were mixed and stirred, then 1,000 mg of lemon concentrate, 300 mg of lime concentrate, 400 mg of agave syrup, 4 mg of grapefruit seed extract, and 1,000 mg of fructooligosaccharide were further mixed and stirred, and 124 mg of natural lemon-lime flavor was further added and mixed and stirred to prepare the first composition.

[0090] Comparative Example 1_ Second composition

[0091] In the above example, the low molecular weight collagen was modified to 3,500 mg and prepared in the same manner as in the example. That is, a second composition was prepared with a reduced content of low molecular weight collagen compared to the example.

[0093] Comparative Example 2_ Third composition

[0094] In the above example, the low molecular weight collagen was modified to 6,500 mg and prepared in the same manner as in the example. That is, a third composition was prepared with a higher content of low molecular weight collagen than in the example.

[0096] Experimental Example 1_ Measurement of Blood Hydroxyproline and Digestive Irritation

[0097] Thirty women in their 50s weighing between 50 and 60 kg were selected and randomly divided into three groups of 10: Group A, Group B, and Group C. Group A was instructed to consume 10 g of the composition prepared in the example once a day at the same time for 10 days, Group B was instructed to consume 10 g of the composition prepared in Comparative Example 1 once a day at the same time for 10 days, and Group C was instructed to consume 10 g of the composition prepared in Comparative Example 2 once a day at the same time for 10 days. Subsequently, the blood levels of hydroxyproline peptide in Groups A, B, and C were measured and the average value was calculated. Furthermore, a sensory evaluation regarding digestive irritation after consumption was conducted. For the sensory evaluation, participants were asked to select one of the following: 'very poor', 'poor', 'average', 'good', or 'very good'. Scores of 1 to 5 were assigned sequentially, and the average value was calculated.

[0098] The average values ​​of each result are as shown in Table 1 below.

[0099] division Blood hydroxyproline peptide (nmol / ml) before intake Blood hydroxyproline peptide (nmol / ml) after ingestion Digestive irritation (heartburn, bloating, vomiting, etc.) Group A 42.6 68.1 4.03 Group B 43.1 52.8 4.36 Group C 42.5 73.3 2.49

[0100] As shown in Table 1 above, it can be seen that as the content of low molecular weight collagen increases, the amount of hydroxyproline peptide increases, and collagen synthesis in the body becomes active. However, it can be confirmed that when the content of low molecular weight collagen is high, exceeding 5,000 mg, it can irritate the digestive system and cause gastrointestinal disorders.

[0102] Comparative Example 3_ 4th composition

[0103] In the above example, the product was prepared in the same manner as the example, excluding hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, glutathione, and yeast extract.

[0105] Comparative Example 4_ 5th composition

[0106] In the above example, the composition was prepared in the same manner as the example by modifying the content to 90 mg of hyaluronic acid, 80 mg of prolastin, 180 mg of vitamin C, 0.5 mg of milk ceramide, 0.005 mg of biotin, and 0.05 mg of glutathione yeast extract. That is, a fifth composition was prepared in which the content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, and glutathione yeast extract was reduced compared to the example.

[0108] Comparative Example 5_ Composition 6

[0109] In the above example, the composition was prepared in the same manner as the example by modifying the content to 140 mg of hyaluronic acid, 120 mg of prolastin, 300 mg of vitamin C, 4 mg of milk ceramide, 0.1 mg of biotin, and 1.5 mg of glutathione yeast extract. That is, a sixth composition was prepared in which the content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, and glutathione yeast extract was higher than that of the example.

[0111] Experimental Example 2_ Measurement of Blood Hydroxyproline

[0112] Forty women in their 50s with a weight of 50 to 60 kg were selected and randomly divided into four groups of ten: Group A, Group B, Group C, and Group D. Group A was instructed to consume 10 g of the composition prepared in the example once a day at the same time for 10 days; Group B was instructed to consume 10 g of the composition prepared in Comparative Example 3 once a day at the same time for 10 days; Group C was instructed to consume 10 g of the composition prepared in Comparative Example 4 once a day at the same time for 10 days; and Group D was instructed to consume 10 g of the composition prepared in Comparative Example 5 once a day at the same time for 10 days. Subsequently, blood hydroxyproline peptide levels in Groups A, B, C, and D were measured, and the average value was calculated.

[0114] The average values ​​of the blood hydroxyproline measurement results are as shown in Table 2 below.

[0115] division Blood hydroxyproline peptide (nmol / ml) before intake Blood hydroxyproline peptide (nmol / ml) after ingestion Group A 41.4 58.3 Group B 40.3 43.5 Group C 39.8 47.9 Group D 41.2 62.7

[0116] As shown in Table 2 above, the experimental results indicate that as the content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, glutathione, and yeast extract increases, the amount of hydroxyproline peptide increases, indicating that collagen synthesis in the body is actively carried out.

[0117] The results of Experimental Example 2 show that hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, glutathione, and yeast extract included in the first composition of the example have a significant effect on the synthesis of collagen.

[0119] Experimental Example 3_Skin Condition Measurement and Sensory Evaluation of Skin Condition

[0120] Skin moisture and elasticity of groups A, B, C, and D in Experimental Example 2 were measured to evaluate the skin moisturizing effect and skin elasticity effect, and a sensory evaluation of the skin condition was conducted.

[0121] Skin moisture and elasticity were expressed as a percentage of the increase in moisture and elasticity by comparing the average values ​​measured before and after the intake of each composition. Skin moisture was measured using commonly used skin moisture meters and skin elasticity meters.

[0122] For the sensory evaluation of skin condition, one of 'very poor', 'poor', 'average', 'good', or 'very good' was selected, and scores of 1 to 5 points were assigned sequentially, after which the average value was calculated.

[0123] The results of the skin condition measurement and skin condition sensory evaluation are as shown in Table 3 below.

[0124] division moisturizing effect Elasticity effect skin troubles itching Group A 35.1% 41.9% 4.25 4.65 Group B 10.4% 9.7% 3.57 4.01 Group C 15.5% 16.4% 3.61 4.31 Group D 38.8% 38.1% 2.51 2.07

[0125] As a result of the experiment, as shown in Table 3 above, it was confirmed that the moisturizing and elasticity effects increased as the content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, and glutathione yeast extract increased. However, it was confirmed that Group D, which had the highest content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, and glutathione yeast extract, had skin troubles and itching at a moderate to poor level.

[0127] Experimental Example 4_Skin Condition Measurement and Sensory Evaluation of Skin Condition

[0128] Sensory evaluation of digestive irritation was conducted on groups A, B, C, and D in Experimental Example 2. For the sensory evaluation of digestive irritation, participants were asked to select one of 'very bad', 'bad', 'average', 'good', or 'very good', and scores of 1 to 5 points were assigned sequentially, after which the average value was calculated.

[0129] The average value of the results is as shown in Table 4 below.

[0130] division Digestive irritation (heartburn, bloating, vomiting, etc.) Group A 4.15 Group B 4.01 Group C 4.03 Group D 2.48

[0131] As shown in Table 4 above, the experimental results confirmed that Group D, which had the highest content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, glutathione, and yeast extract, was at a poor to moderate level in terms of digestive irritation.

[0133] According to the results of Experimental Examples 2 to 4, it was confirmed that the higher the content of hyaluronic acid, prolastin, vitamin C, milk ceramide, biotin, glutathione, and yeast extract, the better the skin improvement effect, but at high concentrations, skin troubles, skin itching, and gastrointestinal disorders may occur.

[0135] Comparative Example 6_ 7th Composition

[0136] In the above example, the product was prepared in the same manner as the example, except for the lemon concentrate, lime concentrate, agave syrup, fructooligosaccharide, and natural lemon-lime flavor.

[0138] Comparative Example 7_ Composition 8

[0139] In the above example, the ingredients were modified to 500 mg lemon concentrate, 100 mg lime concentrate, 200 mg agave syrup, 700 mg fructooligosaccharide, and 60 mg natural lemon-lime flavor, and prepared in the same manner as in the example.

[0141] Comparative Example 8_ Composition 9

[0142] In the above example, the ingredients were modified to 1,500 mg lemon concentrate, 500 mg lime concentrate, 600 mg agave syrup, 1,300 mg fructooligosaccharide, and 180 mg natural lemon-lime flavor, and prepared in the same manner as in the example.

[0144] Experimental Example 5_ Taste and Aroma of the Composition

[0145] The sensory evaluation was conducted by 30 trained sensory evaluators who comprehensively assessed sweetness, bitterness, fishy taste, sourness, and aroma. They were asked to select one of the following scores: 'very bad', 'bad', 'average', 'good', or 'very good', and then scores from 1 to 5 were assigned sequentially, after which the average value was calculated.

[0146] The average value of the results is as shown in Table 5 below.

[0147] division Preference Examples 4.7 Comparative Example 6 1.2 Comparative Example 7 2.3 Comparative Example 8 3.1

[0148] As shown in Table 5 above, the experimental results showed that the 7th composition of Comparative Example 6 had very poor to poor levels of sweetness, bitterness, fishy taste, sourness, and aroma, while the 8th and 9th compositions of Comparative Examples 7 and 8 had poor to average levels of sweetness, bitterness, fishy taste, sourness, and aroma.

[0149] The first composition of the example was confirmed to have the highest preference, with sweetness, bitterness, fishy taste, sourness, and aroma at a good to very good level.

[0151] According to the experimental results described above, the inner beauty composition for skin improvement containing low molecular weight collagen according to one embodiment of the present invention has a high collagen absorption rate and can actively promote collagen synthesis when ingested, and can provide excellent skin improvement effects such as skin elasticity and hydration.

[0152] Furthermore, it causes no side effects upon consumption and has excellent taste and aroma, making it easy to consume.

[0154] Although the structure and features of the present invention have been described above based on embodiments thereof, the present invention is not limited thereto. It is obvious to those skilled in the art that various changes or modifications can be made within the spirit and scope of the present invention, and therefore, it is noted that such changes or modifications fall within the scope of the appended claims.

Claims

Claim 1 An inner beauty composition for skin improvement containing low molecular weight collagen, comprising 11,000 to 12,000 parts by weight of purified water, 4,950 to 5,050 parts by weight of low molecular weight collagen, 90 to 110 parts by weight of prolastin, 150 to 250 parts by weight of vitamin C, 110 to 130 parts by weight of hyaluronic acid, 60 to 70 parts by weight of enzyme-treated stevia, 1 to 3 parts by weight of vitamin mineral mix, 1 to 3 parts by weight of milk ceramide, 0.01 to 0.03 parts by weight of biotin, 0.1 to 0.3 parts by weight of glutathione yeast extract, and 50 to 60 parts by weight of hydrated citric acid. Claim 2 delete Claim 3 An inner beauty composition for skin improvement containing low molecular weight collagen according to claim 1, further comprising lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract, fructooligosaccharide, and natural lemon-lime scent. Claim 4 An inner beauty composition for skin improvement containing low molecular weight collagen according to claim 3, comprising 950 to 1,050 parts by weight of lemon concentrate, 250 to 350 parts by weight of lime concentrate, 350 to 450 parts by weight of agave syrup, 3 to 5 parts by weight of grapefruit seed extract, 950 to 1,050 parts by weight of fructooligosaccharide, and 115 to 135 parts by weight of natural lemon-lime scent. Claim 5 A first mixing step of producing a first mixture by mixing 11,000 to 12,000 parts by weight of purified water, 4,950 to 5,050 parts by weight of low molecular weight collagen, 90 to 110 parts by weight of prolastin, 150 to 250 parts by weight of vitamin C, 110 to 130 parts by weight of hyaluronic acid, 60 to 70 parts by weight of enzyme-treated stevia, 1 to 3 parts by weight of vitamin mineral mix, 1 to 3 parts by weight of milk ceramide, 0.01 to 0.03 parts by weight of biotin, 0.1 to 0.3 parts by weight of glutathione yeast extract, and 50 to 60 parts by weight of hydrated citric acid; a first stirring step of stirring the first mixture at 60 to 70°C for 10 minutes; lemon concentrate, lime concentrate, agave syrup, grapefruit seed extract A method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen, comprising: a second mixing step of producing a second mixture by adding fructooligosaccharides; a second stirring step of stirring the second mixture at 60 to 70 degrees for 15 minutes; a third mixing step of producing a third mixture by adding a natural lemon-lime scent to the stirred second mixture; and a third stirring step of stirring the third mixture at 60 to 70 degrees for 10 minutes. Claim 6 A method for preparing an inner beauty composition for skin improvement containing low molecular weight collagen, characterized in that, in claim 5, the third mixture has a sugar content of 37 to 47 brix and a hydrogen ion concentration index (PH) of 3.9 to 4.9.

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