Functional cosmetic composition that simultaneously provides moisturizing, skin elasticity, and anti-aging effects

Abstract

The present invention relates to a functional cosmetic composition, and more specifically, to a functional cosmetic composition that provides moisturizing, skin elasticity, and anti-aging effects simultaneously by using melatonin, resveratrol, and bakuchiol as basic active ingredients and sequentially including natural ingredients derived from rare aquatic plants and resins, as well as peptides. The composition of the present invention provides a moisturizing environment that can be maintained for a long time by suppressing moisture loss from the skin surface and stabilizing the skin barrier. In particular, during the night, the skin cell recovery mechanism and antioxidant action work in combination to alleviate oxidative stress caused by the external environment and promote the recovery of skin condition. Furthermore, the composition of the present invention enhances the sustainability of the moisturizing effect by supporting the skin barrier function, thereby maintaining a stable skin moisture state even as time passes after application. As a result, the composition of the present invention exhibits a superior sustained moisturizing effect compared to conventional general moisturizing cosmetics.

Description

Technology Field

[0001] The present invention relates to a functional cosmetic composition, and more specifically, to a functional cosmetic composition that provides moisturizing, skin elasticity, and anti-aging effects simultaneously by using melatonin, resveratrol, and bakuchiol as basic active ingredients and including natural ingredients derived from rare aquatic plants and resins and peptides in stages. Background Technology

[0002] Skin hydration is a key factor in maintaining skin health and improving its appearance; failure to adequately maintain skin moisture levels can lead to various problems such as dryness, roughness, loss of elasticity, and premature aging. However, skin hydration is difficult to sustain for extended periods by simply supplying moisture from the outside; it must be accompanied by mechanisms that inhibit moisture evaporation from the skin surface and stabilize the skin barrier. In particular, transepidermal water loss (TEWL) is a factor that directly affects the sustainability of skin hydration, and a decline in skin barrier function can accelerate moisture loss.

[0003] Meanwhile, skin aging is not caused by a single factor but is the result of the complex interplay of factors, including the accumulation of oxidative stress, the decrease in structural proteins such as collagen and elastin, and reduced cellular vitality due to lowered cellular energy metabolism. This skin aging process manifests outwardly in the form of decreased skin elasticity, wrinkle formation, and uneven skin tone, and is closely linked to a decline in moisturizing function. Accordingly, there has been a continuously increasing demand for functional cosmetic compositions that go beyond simple moisturizing functions to simultaneously provide skin elasticity improvement and anti-aging effects.

[0004] Melatonin is known as a hormone involved in regulating circadian rhythms and is reported to be involved in antioxidant activity and cell repair processes in the skin during the night. In particular, melatonin is recognized as playing a crucial role in the process of skin cells recovering after being damaged by external stimuli. Additionally, resveratrol is a plant-derived polyphenol compound known to have the effect of eliminating free radicals and inhibiting cell damage through its powerful antioxidant activity. Furthermore, bakuchiol is a plant-derived ingredient exhibiting biological activity similar to retinol, and it is attracting attention as a component that contributes to improving skin elasticity by strengthening the skin barrier and promoting collagen synthesis.

[0005] However, since the aforementioned ingredients primarily focus on functions such as antioxidant activity, promotion of cell repair, or improvement of skin structure, they had limitations in strengthening the skin's moisture-retaining structure itself or physically inhibiting moisture loss. In other words, a combination of ingredients focused solely on antioxidants and regeneration was insufficient to effectively block moisture evaporation from the skin surface or maintain a stable moisturizing environment for an extended period.

[0006] Accordingly, to fundamentally improve skin hydration, it is required to introduce additional ingredients that inhibit moisture loss from the skin surface and support barrier function, in addition to ingredients that perform antioxidant and regenerative functions. In particular, naturally derived ingredients that have evolved to minimize moisture loss from the external environment—such as plant-derived components surviving in extreme or aquatic environments, or resinous components secreted by plants—have great potential for use in terms of skin hydration and barrier maintenance. Furthermore, when combined with peptides involved in the energy and collagen metabolism of skin cells, it is expected that not only can the moisturizing effect be sustained, but skin elasticity and anti-aging effects can also be achieved simultaneously.

[0007] Therefore, there is a need for technological development of new functional cosmetic compositions that organically combine moisturizing, skin elasticity, and anti-aging effects, in order to overcome the limitations of existing cosmetic compositions that are limited to moisturizing or anti-aging functions. Prior art literature

[0008] (Patent Document 0001) KR 10-2841933 B (Patent Document 0002) KR 10-2025-0169391 A (Patent Document 0003) KR 10-2829426 B (Patent Document 0004) KR 10-2265730 B The problem to be solved

[0009] The present invention aims to solve the problem that conventional moisturizing cosmetics rely on simple moisture supply, resulting in a lack of sustained moisturizing effect and limitations in skin barrier stabilization and inhibition of moisture loss. Furthermore, existing anti-aging cosmetic compositions are biased toward antioxidant or wrinkle improvement functions, which limits their ability to simultaneously address skin elasticity decline and collagen reduction. Moreover, there was a problem in that they failed to sufficiently consider anti-aging effects linked to the skin's night recovery mechanism. Accordingly, the objective of the present invention is to provide a functional cosmetic composition capable of simultaneously achieving improved sustained moisturizing effect based on inhibition of skin moisture loss, improved skin elasticity through the regulation of collagen metabolism, and inhibition of skin aging through antioxidant and night recovery mechanisms. means of solving the problem

[0010] In order to solve the above problems, the present invention relates to melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica Provides a functional cosmetic composition for enhancing moisturizing power containing an extract.

[0011] It is preferable that the above functional cosmetic composition for enhancing moisturizing power further includes Dragon's Blood extract.

[0012] It is preferable that the above functional cosmetic composition for enhancing moisturizing power further includes Nonapeptide-1.

[0013] It is preferable that the above functional cosmetic composition for enhancing moisturizing power further includes Tripeptide-72. Effects of the invention

[0014] The composition of the present invention provides a long-lasting moisturizing environment by inhibiting moisture loss from the skin surface and stabilizing the skin barrier. In particular, during the night, the skin cell recovery mechanism and antioxidant action work in combination to alleviate oxidative stress caused by the external environment and promote the recovery of skin condition. Furthermore, the composition of the present invention enhances the sustainability of the moisturizing effect by supporting the skin barrier function, thereby maintaining a stable skin moisture state even as time passes after application. As a result, the composition of the present invention exhibits a superior sustained moisturizing effect compared to conventional general moisturizing cosmetics. Specific details for implementing the invention

[0015] The present invention will be described in detail below.

[0016] Based on the observation that skin hydration is difficult to maintain for a long time through simple hydration alone and requires the simultaneous stabilization of the skin barrier and inhibition of moisture loss, the present invention aimed to develop a functional cosmetic composition capable of improving the longevity of hydration and simultaneously providing skin elasticity and anti-aging effects by organically combining ingredients that provide an antioxidant and restorative environment with ingredients that strengthen the moisture retention environment.

[0017] The composition according to the present invention includes melatonin as a component related to the night recovery environment, includes resveratrol which contributes to antioxidant activity, and includes bakuchiol which contributes to strengthening the skin barrier and improving elasticity. In addition, the present invention relates to a giant water lily (which can contribute to inhibiting moisture loss and maintaining a moisturizing environment due to the characteristics of plants surviving in an aquatic environment). Victoria Amazonica By including additional ingredients derived from ), the aim was to supplement the longevity of moisture, which is difficult to sufficiently secure with only the basic active ingredients mentioned above.

[0018] Accordingly, the present invention relates to melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica Provides a functional cosmetic composition for enhancing moisturizing power containing an extract.

[0019] In the above composition, it is preferable that each component be included in the following content ranges based on the total weight of the composition.

[0020] It is preferable that melatonin be included in an amount of 0.00001% to 0.01% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.00001% by weight, is intended to secure a minimum effective content that can contribute to creating a night recovery environment for skin cells and antioxidant activity; if lower than this, the night recovery and anti-aging effects expected as a functional cosmetic may not be sufficiently manifested. The upper limit of the above content range, 0.01% by weight, is a limit considering stability and formulation suitability when applied to cosmetics; if this is exceeded, there is a concern that formulation stability may be reduced or efficiency may decrease due to unnecessary over-injection.

[0021] It is preferable that resveratrol be included in an amount of 0.001% to 1.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.001% by weight, is the minimum effective amount for exhibiting free radical scavenging and antioxidant effects; if it is lower than this, it is difficult to sufficiently exhibit antioxidant synergy effects. The upper limit of the above content range, 1.0% by weight, is a range considering solubility, formulation stability, and potential skin irritation; if it is exceeded, formulation issues or a decrease in usability may occur.

[0022] It is preferable that bakuchiol be included in an amount of 0.01% to 2.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.01% by weight, is the minimum effective amount for exhibiting the effect of improving skin elasticity through strengthening the skin barrier and regulating collagen metabolism. The upper limit of the above content range, 2.0% by weight, is a range that considers stability and usability in the cosmetic formulation; if this is exceeded, formulation stability or sensory quality may be degraded due to unnecessary excessive addition.

[0023] The large water lily used in the present invention ( Victoria Amazonica It is desirable that the ) extract be manufactured to efficiently recover ingredients effective for skin moisturization and barrier maintenance while maintaining the characteristics of aquatic plants. The above-mentioned giant water lily ( Victoria Amazonica The extract can be obtained from leaves, flowers, or a mixture thereof, and the method of preparation may include the following steps.

[0024] First of all, the giant water lily ( Victoria Amazonica After harvesting the leaves and / or flowers of the plant, they are washed with purified water to remove foreign substances, and if necessary, naturally dried or low-temperature dried to obtain a dried product. It is preferable to grind the dried product into a powder state to improve extraction efficiency.

[0025] Subsequently, the powder or raw material in a biological state is immersed in an extraction solvent comprising one or more of purified water, ethanol, or a mixed solvent of purified water and ethanol to perform extraction. At this time, considering the stability of the moisturizing active ingredient, the extraction solvent is preferably an aqueous ethanol solution of 30 to 80 weight%, and the extraction temperature can be selected from room temperature to 70°C, and the extraction time can be selected from 2 to 48 hours.

[0026] After extraction is completed, the extract is filtered or centrifuged to remove solids, and if necessary, vacuum concentration is performed to obtain an extract concentrated with active ingredients. The concentrated extract may also be prepared in powder form through freeze-drying or spray-drying processes to improve formulation stability and shelf life.

[0027] In addition, the above large water lily ( Victoria Amazonica The extract may additionally include filtration, sterilization, or pH adjustment processes to improve skin applicability, and may also be provided dissolved in a cosmetic solvent such as glycerin, butylene glycol, or propanediol as needed.

[0028] Large water lily prepared by the above method ( Victoria Amazonica The extract derived from ) contains components related to moisture retention and inhibition of moisture loss due to the characteristics of plants surviving in an aquatic environment, and can be applied as an active ingredient in the functional cosmetic composition for enhancing moisturizing power of the present invention.

[0029] Large water lily ( Victoria AmazonicaIt is preferable that the extract be included in an amount of 0.01% to 5.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.01% by weight, is the minimum amount required to exhibit the effects of inhibiting moisture loss and maintaining a moisturizing environment due to the characteristics of the aquatic plant-derived component. The upper limit of the above content range, 5.0% by weight, is a range that takes into account the physical properties, stability, and sensory quality of the formulation; exceeding this may result in increased viscosity or formulation instability.

[0030] In addition, the functional cosmetic composition for enhancing moisturizing power according to the present invention preferably further includes Dragon's Blood extract, a resin component derived from plants, to more effectively suppress moisture loss from the skin surface and assist in barrier stabilization. Dragon's Blood extract can contribute to reducing transepidermal water loss and maintaining a more stable moisturizing state by forming a protective film on the skin surface or assisting in the skin's protection against the external environment. That is, by additionally including Dragon's Blood, the composition of the present invention can enhance the effect of maintaining a moisturizing environment even after a period of time, going beyond temporary moisturization immediately after application.

[0031] The Dragon's Blood extract used in the present invention is preferably prepared using resin naturally secreted from plants as a raw material. The Dragon's Blood may be obtained by collecting resin exuded from the stems or branches of plants of the genus Dracaena.

[0032] Specifically, after collecting the Dragon's Blood resin, a sieving or simple washing process is performed to remove foreign substances. Subsequently, it is desirable to grind the resin or prepare it in the form of small pieces to increase extraction efficiency.

[0033] The prepared resin can be extracted by immersing it in an extraction solvent containing one or more of purified water, ethanol, or a mixed solvent of purified water and ethanol. At this time, considering the solubility and stability of the resin components, it is preferable to use an aqueous ethanol solution of 50 to 95 weight%, and the extraction temperature can be selected from room temperature to 60°C, and the extraction time can be selected from 1 to 48 hours.

[0034] After extraction is complete, the extract is filtered or centrifuged to remove insoluble solids, and if necessary, concentrated under reduced pressure to obtain the dragon's blood extract. The extract may be prepared in powder form through freeze-drying or spray-drying processes to improve formulation stability and shelf life.

[0035] In addition, the above dragon's blood extract may further include a process of sterilization, decolorization, pH adjustment, or redissolution in a cosmetic solvent such as glycerin or butylene glycol to improve skin applicability.

[0036] The dragon's blood-derived extract prepared by the above method is a resin component derived from a plant, which can form a protective film on the skin surface to inhibit moisture loss and contribute to the stabilization of the skin barrier.

[0037] In the functional cosmetic composition for enhancing moisturizing power according to the present invention, it is preferable that the dragon's blood extract be included in an amount of 0.001% by weight to 3.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.001% by weight, represents the minimum effective content for the dragon's blood resin component to form a protective film on the skin surface and exhibit the effect of inhibiting transepidermal water loss. At a content lower than this, the effects of supporting the skin barrier and improving the longevity of moisturization may not be sufficiently expressed. The upper limit of the above content range, 3.0% by weight, is a range that considers stability, usability, and color effects in the cosmetic formulation; exceeding this range may lead to an increase in the viscosity of the formulation, a change in color, or a deterioration in sensory quality. Furthermore, the inclusion of an excessive amount of the resin component may reduce efficiency relative to the moisturizing effect.

[0038] Furthermore, the functional cosmetic composition for enhancing moisturizing power according to the present invention preferably further includes Nonapeptide-1 to further enhance the improvement of skin condition and anti-aging effects. Nonapeptide-1 can contribute to improving the appearance of dullness or fatigue caused by dry skin by assisting functions related to the energy metabolism activity of skin cells and skin vitality. Additionally, when combined with melatonin and resveratrol, the stabilization of skin condition can be further promoted through a night recovery environment and antioxidant synergy. Therefore, the additional inclusion of Nonapeptide-1 can contribute to enhancing effects in terms of maintaining skin vitality and anti-aging, as well as moisturizing duration.

[0039] The Nonapeptide-1 used in the present invention is a peptide composed of nine amino acids and can be provided in the form of a synthetic peptide commercially available as a cosmetic ingredient. The Nonapeptide-1 can generally be produced by solid-phase peptide synthesis (SPPS) and can be provided as a commercial ingredient in a state dissolved in an aqueous solution, glycerin, or butylene glycol.

[0040] Specifically, Nonapeptide-1 can be obtained in powder or solution form from a company specializing in peptide synthesis, and can be applied to cosmetic formulations after undergoing sterilization, filtration, or dilution processes as needed. Since the stability and reproducibility of Nonapeptide-1 in this form as a raw material for cosmetics are ensured, it is suitable for application in the functional cosmetic composition of the present invention.

[0041] In the functional cosmetic composition for enhancing moisturizing power according to the present invention, it is preferable that Nonapeptide-1 be included in an amount of 0.0001% to 0.05% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.0001% by weight, represents the minimum effective content for improving skin condition and exhibiting anti-aging effects by assisting functions related to the energy metabolism activity of skin cells and skin vitality of Nonapeptide-1. At a content lower than this, the night recovery and antioxidant synergies resulting from co-administration with melatonin and resveratrol may not be sufficiently expressed. The upper limit of the above content range, 0.05% by weight, is a range that considers stability in the cosmetic formulation, cost-efficiency, and the benefits of excessive addition based on the characteristics of the peptide; exceeding this may result in a decrease in formulation stability or efficiency relative to effect. Furthermore, excessive peptide content may lead to unnecessary constraints in terms of formulation design.

[0042] In addition, the functional cosmetic composition for enhancing moisturizing power according to the present invention preferably further includes Tripeptide-72 to further enhance the effects of skin structure stabilization and elasticity improvement. Tripeptide-72 participates in collagen metabolism related to skin elasticity and acts to promote collagen synthesis or inhibit collagen degradation pathways, thereby contributing to supporting the structural support of the skin and alleviating the decline in elasticity. In particular, combined use with bakuchiol allows the skin barrier and structure improvement effects to work complementarily, which can help maintain a moisturizing environment and increase the perceived effect of skin elasticity and anti-aging.

[0043] The tripeptide-72 used in the present invention is a peptide composed of three amino acids and can be provided in the form of a synthetic peptide commercially available as a cosmetic ingredient. The tripeptide-72 is generally manufactured by solid-phase peptide synthesis (SPPS) and can be provided in the form of a powder or solution after undergoing a purification process following manufacturing.

[0044] Specifically, Tripeptide-72 can be obtained as a high-purity peptide raw material from a company specializing in peptide synthesis, and, if necessary, can be provided in a state dissolved in a cosmetic solvent such as purified water, glycerin, butylene glycol, or propanediol. Tripeptide-72 in this form has excellent formulation stability and reproducibility, making it suitable for application in the functional cosmetic composition for enhancing moisturizing power according to the present invention.

[0045] In the functional cosmetic composition for enhancing moisturizing power according to the present invention, it is preferable that tripeptide-72 be included in an amount of 0.0001% to 0.1% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.0001% by weight, represents the minimum effective content for tripeptide-72 to participate in collagen metabolism to exhibit effects of skin structure stabilization and elasticity improvement. At a content lower than this, the effects of maintaining skin elasticity and improving structural support may not be sufficiently expressed. The upper limit of the above content range, 0.1% by weight, is a range considering stability, cost-efficiency, and peptide characteristics in the cosmetic formulation; exceeding this may result in reduced efficiency relative to effect or cause unnecessary constraints in formulation design. Furthermore, an excessive peptide content may affect formulation stability or sensory quality.

[0046] The above components of the present invention can be used as a functional cosmetic composition for improving moisturizing, skin elasticity, and anti-aging effects.

[0047] The above-mentioned component of the cosmetic composition of the present invention may be included in an amount of 0.01 to 50% by weight based on the total weight of the composition, preferably 0.1 to 20% by weight, and more preferably 1 to 10% by weight. Within this content range, appropriate formulation stability can be secured and the desired effect can be expected.

[0048] In addition to the above-mentioned components of the present invention, the composition of the present invention may be used by additionally including known natural extracts or other components for the effects of antioxidant, anti-aging, and skin regeneration promotion, to the extent that such effects are not inhibited.

[0049] In addition, the above composition may be formed into a specific formulation by including auxiliary agents or carriers commonly used in the fields of cosmetics or dermatology, such as fatty substances, organic solvents, solvents, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion chelating agents and chelating agents, preservatives, vitamins, blockers, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents.

[0050] The cosmetic composition of the present invention can be prepared in any formulation conventionally manufactured in the art, for example, as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, pack, soap, surfactant-containing cleansing, oil, and spray, but is not limited thereto. More specifically, it can be prepared in formulations such as a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, a powder, a compact, a lip gloss, a lipstick, an eyeshadow, a shampoo, and a rinse.

[0051] In the case where the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0052] In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0053] When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0054] In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used as carrier components.

[0055] In the case where the formulation of the present invention is a cleansing agent containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0056] The present invention will be explained in more detail below through specific embodiments. The following embodiments describe a preferred embodiment of the present invention, and the scope of the present invention is not to be interpreted as being limited by the matters described in the following embodiments.

[0057] [Example]

[0058] Example 1

[0059] Melatonin, Resveratrol, Bakuchiol, and giant water lily ( Victoria AmazonicaA functional cosmetic composition containing ) extract was prepared.

[0060] Specifically, an aqueous phase containing purified water, glycerin, and butylene glycol was prepared and stirred while heating it to approximately 70°C. Separately, an oil phase containing vegetable oil, fatty acid ester, and an emulsifier was heated to the same temperature, and then the oil phase was added to the aqueous phase while high-speed stirring or homogenization was performed to form an emulsion.

[0061] Subsequently, the formed emulsion was slowly cooled while continuously stirring to obtain a semi-solid composition with increased viscosity, and during the cooling process, in a temperature range of 40°C or lower, melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica The extracts were added sequentially and stirred sufficiently to disperse them uniformly. Afterwards, thickeners, stabilizers, or texture improvers were added as needed to adjust the viscosity and spreadability suitable for a cream formulation.

[0062] Finally, after adjusting the pH of the entire composition to a range suitable for the skin, the functional cosmetic composition in the form of a cream was completed by removing air through a degassing process and filling it into a container.

[0063] Example 2

[0064] A functional cosmetic composition was prepared in the same manner as in Example 1, but the composition additionally included Dragon's Blood extract.

[0065] Specifically, an aqueous phase containing purified water, glycerin, and butylene glycol was prepared and stirred while heating to around 70°C, and separately an oil phase containing vegetable oil, fatty acid ester, and an emulsifier was heated to the same temperature, and then high-speed stirring or homogenization was performed while adding the oil phase to the aqueous phase to form an emulsion.

[0066] Subsequently, the formed emulsion is slowly cooled while continuously stirring, and during the cooling process, in a temperature range of 40℃ or lower, melatonin, resveratrol, bakuchiol, giant water lily ( Victoria Amazonica Dragon's blood extract was added sequentially along with the extract and uniformly dispersed. Then, a thickening agent, a stabilizer, or a texture improver was added as needed to adjust the viscosity and spreadability suitable for a cream formulation, and a functional cosmetic composition in a cream formulation was completed by filling it into a container after pH adjustment and degassing processes.

[0067] Example 3

[0068] A functional cosmetic composition was prepared in the same manner as in Example 2, but the composition additionally included Nonapeptide-1.

[0069] Specifically, after preparing the aqueous and oil phases separately and performing an emulsification process at the same temperature, while cooling the emulsion in a temperature range of 40℃ or lower, melatonin, resveratrol, bakuchiol, giant water lily ( Victoria Amazonica ) extract and Dragon's Blood extract were added.

[0070] Subsequently, during the final cooling step, Nonapeptide-1 was added in a state of pre-dissolution in purified water or butylene glycol and stirred sufficiently to uniformly disperse it within the composition. Then, thickeners and texture improvers were selectively added to adjust physical properties suitable for a cream formulation, and a functional cosmetic composition in the form of a cream was completed through pH adjustment and degassing processes.

[0071] Example 4

[0072] A functional cosmetic composition was prepared in the same manner as in Example 3, but the composition additionally included Tripeptide-72.

[0073] Specifically, through emulsification and cooling processes, melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica After adding ) extract, dragon's blood extract and nonapeptide-1, tripeptide-72 was additionally added during the final cooling step and stirred sufficiently to maintain a uniform dispersion state.

[0074] Subsequently, a thickening agent or a stabilizer was optionally added to control the viscosity and spreadability of the composition, and a pH adjustment and degassing process was performed before filling the composition into a container to complete a functional cosmetic composition in the form of a cream.

[0075] Comparative Example 1

[0076] A commercially available general moisturizing cream cosmetic from Company C was used. The said cosmetic is manufactured in a cream formulation containing conventional moisturizing ingredients such as hyaluronic acid.

[0077] Comparative Example 2

[0078] A functional cosmetic composition containing only melatonin, resveratrol, and bakuchiol was prepared.

[0079] The manufacturing method was the same as in Example 1, in which an aqueous phase and an oil phase were prepared separately and an emulsification process was performed; after adding melatonin, resveratrol, and bakuchiol at a temperature of 40°C or lower during the cooling process, a cream-form cosmetic composition was completed through viscosity adjustment, pH adjustment, and degassing processes. However, the above composition contains water lily ( Victoria Amazonica It did not contain ) extract, Dragon's Blood, or peptides.

[0080] Experimental Example 1. Evaluation of Transepidermal Water Loss (TEWL) Reduction Effect (Moisture Durability)

[0081] Transepidermal Water Loss (TEWL) was measured to evaluate the persistence of skin hydration. The subjects consisted of healthy adult men and women, and the test site was set as the inner arm (forearm). Before the test, the subjects were allowed to stabilize for a certain period to adapt to the laboratory environment, and no separate cosmetics were applied to the test site during the test period.

[0082] The compositions of each example and comparative example were applied by dividing them into equal area sections (e.g., 4 cm × 4 cm), and the application amount was a fixed amount per area (e.g., 2 mg / cm²). 2 It was measured and applied to achieve the desired result. After application, the test area was allowed to absorb naturally without rubbing, and TEWL was measured immediately after application and at 2, 6, and 12 hours after application. TEWL was measured at least three times per section using commonly used TEWL measuring equipment (such as Tewameter series equipment), and the average value was calculated.

[0083] The laboratory environment maintained temperature and relative humidity within a certain range (e.g., 20–25°C, relative humidity 40–60%), and measurements were performed under the same conditions. The measurement results were converted into a percentage change in TEWL or a percentage reduction in TEWL compared to TEWL before application. If necessary, statistical significance can be analyzed using standard statistical methods (e.g., t-test or ANOVA) based on repeated measurements within the same subject.

[0084] division 2 hours 6 hours 12 hours Example 1 28 22 15 Example 2 35 30 24 Example 3 38 34 29 Example 4 42 38 33 Comparative Example 1 12 5 1 Comparative Example 2 18 10 4

[0085] As shown in Table 1 above, Comparative Example 1 exhibited only a temporary moisturizing effect immediately after application, and the effect decreased rapidly over time. On the other hand, Example 1 is a large water lily ( Victoria AmazonicaThe moisture loss inhibition effect was significantly improved by the inclusion of the extract, and in Example 2, the moisturizing duration was further improved by the protective barrier formation effect of Dragon's Blood. In particular, Examples 3 and 4 showed the most excellent TEWL reduction effect due to the addition of peptides.

[0086] Experimental Example 2. Evaluation of the effect of increasing moisture content in the stratum corneum (moisturizing power)

[0087] To evaluate the moisture content of the stratum corneum, a stratum corneum moisture measuring device (such as a Corneometer series device) was used. The selection of subjects and the setting of the test site were performed in the same manner as in Experimental Example 1, and measurements were conducted after the subjects were acclimatized to the laboratory environment for a certain period of time prior to the test.

[0088] Each example and comparative example composition has the same application amount (e.g., 2 mg / cm²) in the same area section. 2 It was applied. After measuring the moisture content before application as a reference value, the moisture content of the stratum corneum was measured at 2 and 6 hours (or 12 hours) after application. Moisture content measurements were repeated at least 3 times per section, and the average value was calculated.

[0089] The measurement results were calculated as the percentage increase in moisture content compared to before application, and reproducibility was confirmed by performing repeated tests under the same conditions as necessary. The laboratory temperature and relative humidity were maintained within the same range as the TEWL test.

[0090] division Moisture content increase rate (%) Example 1 32 Example 2 39 Example 3 41 Example 4 45 Comparative Example 1 8 Comparative Example 2 15

[0091] As shown in Table 2 above, Example 1 showed a significant increase in the moisture content of the stratum corneum compared to the comparative example, and the improvement in moisturizing power was even more pronounced in Example 2, which contained Dragon's Blood. Examples 3 and 4, which contained peptides, showed the highest increase in moisture content, confirming that the moisturizing power enhancement effect increased stepwise.

[0092] Experimental Example 3. Evaluation of Skin Elasticity Improvement Effect

[0093] To evaluate the skin elasticity improvement effect, the elasticity index (R2) was measured using skin elasticity measuring equipment (such as cutometer-type devices). The subjects consisted of healthy adult men and women, and the test site could be selected from either the forearm or the face (e.g., cheek area). For the long-term application test, subjects were restricted from using functional products other than the test product on the test site during the test period.

[0094] Each example and comparative example composition is applied to a designated test site in a fixed amount (e.g., 2 mg / cm²). 2 Alternatively, based on a single pump discharge amount, it was applied twice a day (morning / evening), and the total application period was set to 4 weeks. Skin elasticity index (R2) was measured using a Cutometer before application (week 0) and at 2 and 4 weeks of application. Measurements were performed on the same area under the same conditions, and the average value was calculated by repeating the measurement at least 3 times at each time point.

[0095] The results were calculated as the percentage increase in the elasticity index compared to before application. If necessary, statistical significance can be determined using standard statistical analysis methods (e.g., ANOVA) with repeated measurements.

[0096] division Elasticity growth rate (%) Example 1 15 Example 2 18 Example 3 22 Example 4 28 Comparative Example 1 3 Comparative Example 2 7

[0097] As shown in Table 3 above, the examples containing bakuchiol showed superior skin elasticity improvement effects compared to the comparative example, and in particular, the elasticity improvement effect due to collagen metabolism regulation was greatest in Examples 3 and 4 containing nonapeptide-1 and tripeptide-72.

[0098] Experimental Example 4. Evaluation of Antioxidant and Anti-aging Effects

[0099] To evaluate antioxidant and anti-aging effects, the inhibition rate of reactive oxygen species (ROS) generation was measured. ROS evaluation can be performed using cell-based tests or skin model tests; in this experiment, a cell-based test using human-derived skin cells (e.g., human dermal fibroblasts or keratinocytes) was conducted.

[0100] Cells were inoculated into a culture plate at an appropriate density (e.g., a 96-well plate) and stabilized culture was performed. Subsequently, the compositions of each example and comparative example were diluted based on the active ingredient and treated to the cells (e.g., treated at a specific concentration). After pretreatment for a certain period, oxidative stress-inducing substances (e.g., H2O2 or UV irradiation) were applied to induce ROS production. The amount of ROS produced was measured using a conventional fluorescent probe (e.g., DCF-DA), and the ROS inhibition rate (%) compared to the untreated control group was calculated based on fluorescence intensity.

[0101] Each condition was performed with at least three repetitions, and the measurements can be summarized as mean ± standard deviation. Statistical significance can be tested using standard analysis methods.

[0102] division ROS Inhibition Rate (%) Example 1 45 Example 2 48 Example 3 52 Example 4 58 Comparative Example 1 10 Comparative Example 2 28

[0103] As shown in Table 4 above, an antioxidant effect was also confirmed in Comparative Example 2 containing melatonin and resveratrol, but the giant water lily ( Victoria Amazonica In Examples 1 to 4, in which ), Dragon's Blood and peptides were added stepwise, the antioxidant and anti-aging effects were significantly enhanced. In particular, Example 4 showed the highest ROS inhibition rate.

Claims

Claim 1 Melatonin, Resveratrol, Bakuchiol, giant water lily ( Victoria Amazonica A cosmetic composition with skin moisturizing, skin elasticity, and antioxidant functions comprising ) extract, Dragon's Blood extract, and Nonapeptide-1. Claim 2 delete Claim 3 delete Claim 4 A cosmetic composition with skin moisturizing, skin elasticity, and antioxidant functions according to claim 1, characterized in that the composition further comprises Tripeptide-72. Claim 5 delete

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