Functional cosmetic composition providing simultaneous wrinkle improvement and skin whitening effects

Abstract

The present invention relates to a functional cosmetic composition, and more specifically, to a functional cosmetic composition that provides both wrinkle improvement and whitening effects simultaneously by using melatonin, resveratrol, and bakuchiol as basic active ingredients and sequentially including ingredients derived from rare aquatic plants, plant-derived resin ingredients, and peptides. The composition of the present invention exhibits a wrinkle improvement effect by alleviating skin wrinkle depth and roughness through the regulation of collagen metabolism and the inhibition of photoaging-inducing pathways. Additionally, it provides a whitening effect by acting on the melanin production, migration, and accumulation pathways to improve skin brightness and tone uniformity. In particular, by sequentially including ingredients derived from aquatic plants, resin ingredients, and peptides in addition to the basic active ingredients, it exhibits a synergistic effect in which wrinkle improvement and whitening effects are mutually enhanced.

Description

Technology Field

[0001] The present invention relates to a functional cosmetic composition, and more specifically, to a functional cosmetic composition that provides wrinkle improvement and whitening effects simultaneously by including melatonin, resveratrol, and bakuchiol as basic active ingredients, and ingredients derived from rare aquatic plants, resin ingredients derived from plants, and peptides in stages. Background Technology

[0002] Skin aging is a biological phenomenon that progresses through the complex interplay of various factors, including external environmental factors such as ultraviolet (UV) radiation, the accumulation of free radicals, the decrease in structural proteins like collagen and elastin, and increased melanin production. In particular, photoaging caused by UV radiation is known to be a major cause of wrinkle formation and skin roughness by increasing the expression of collagen-degrading enzymes in the dermis and exacerbating oxidative stress in skin cells. Over time, this skin aging process manifests as a decline in skin elasticity and the formation of fine and deep wrinkles; simultaneously, increased melanin production and accumulation lead to cosmetic issues such as melasma, blemishes, pigmentation, and uneven skin tone.

[0003] As such, wrinkle formation and pigmentation are not independent phenomena but often proceed simultaneously due to common aging mechanisms, such as increased reactive oxygen species, inflammatory responses, and decreased cellular metabolism. Therefore, wrinkle improvement and whitening can be understood as different aspects of the same technical challenge of improving skin aging, and there has been a continuous need for functional cosmetic compositions that consider both simultaneously.

[0004] Conventional functional cosmetics have often focused on either wrinkle improvement or whitening. For instance, while retinoid-based ingredients intended for wrinkle reduction are effective in promoting collagen synthesis, they often cause side effects such as skin irritation, erythema, and peeling, limiting their long-term use. Meanwhile, cosmetics aimed at whitening focus on inhibiting melanin production or blocking melanin migration; however, when used alone, they suffer from slow onset of effects or limited duration of efficacy. Additionally, although numerous attempts have been made to achieve both wrinkle improvement and whitening simultaneously, most of these merely combined functional ingredients in parallel, resulting in limitations in fully realizing the synergistic effects derived from the interactions between the ingredients.

[0005] Melatonin is a hormone associated with the human body's circadian rhythm and is known to be a component involved in cell repair and antioxidant activity in the skin during the night. In particular, melatonin is reported to alleviate oxidative damage to skin cells and contribute to the recovery of skin condition through its ability to scavenge free radicals. Resveratrol is a plant-derived polyphenol compound that has been reported to provide cell protection and inhibit photoaging through its powerful antioxidant activity, and is utilized in various studies related to skin aging. Bakuchiol is a plant-derived ingredient exhibiting biological activity similar to retinol; it is attracting attention for its advantages of regulating collagen metabolism and contributing to wrinkle improvement while causing less skin irritation compared to retinoid-based ingredients.

[0006] However, the aforementioned ingredients are primarily effective in terms of antioxidant activity or collagen metabolism regulation, and thus have limitations in simultaneously stabilizing skin structure and regulating melanin production and pigmentation. In other words, to achieve both wrinkle improvement and whitening at the same time, a complex approach is required that goes beyond simple antioxidant or collagen synthesis-promoting effects and acts on the entire skin microenvironment to multifacetedly regulate aging-related pathways.

[0007] Accordingly, there has been a continuous increase in technical demand for multi-functional cosmetic compositions capable of simultaneously achieving wrinkle improvement and whitening while maintaining formulation stability, skin applicability, and user experience. In particular, there is a need for research and development on compositions that can exhibit synergistic effects beyond simple parallel combinations by systematically combining ingredients with different mechanisms of action. Prior art literature

[0008] (Patent Document 0001) KR 10-2841933 B (Patent Document 0002) KR 10-2025-0169391 A (Patent Document 0003) KR 10-2829426 B (Patent Document 0004) KR 10-2265730 B The problem to be solved

[0009] The present invention aims to provide a functional cosmetic composition capable of effectively improving wrinkle formation and skin roughness caused by skin aging. Additionally, the invention aims to provide a composition that provides a whitening effect by alleviating skin tone unevenness caused by melanin production and accumulation. Furthermore, the invention aims to provide a functional cosmetic composition capable of simultaneously achieving wrinkle improvement and whitening functions while exhibiting continuous and stable efficacy through synergistic effects between ingredients. means of solving the problem

[0010] In order to solve the above problems, the present invention relates to melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica Provides a functional cosmetic composition for wrinkle improvement and whitening containing an extract.

[0011] It is preferable that the above functional cosmetic composition for wrinkle improvement and whitening further includes Dragon's Blood extract.

[0012] It is preferable that the above functional cosmetic composition for wrinkle improvement and whitening further includes Nonapeptide-1.

[0013] It is preferable that the above functional cosmetic composition for wrinkle improvement and whitening further includes Tripeptide-72. Effects of the invention

[0014] The composition of the present invention exhibits a wrinkle-improving effect by alleviating wrinkle depth and roughness through the regulation of collagen metabolism and the inhibition of photoaging-inducing pathways. Additionally, it provides a whitening effect by acting on the pathways of melanin production, migration, and accumulation to improve skin brightness and tone uniformity. In particular, by sequentially including aquatic plant-derived ingredients, resin-derived ingredients, and peptides in addition to the basic active ingredient, it exhibits a synergistic effect in which wrinkle improvement and whitening effects are mutually enhanced. Specific details for implementing the invention

[0015] The present invention will be described in detail below.

[0016] Based on the observation that skin aging is not caused by a single factor but rather manifests as wrinkle formation and pigmentation due to the complex interplay of factors such as the accumulation of oxidative stress, collagen reduction, and increased melanin production, the present invention aimed to develop a functional cosmetic composition capable of simultaneously achieving wrinkle improvement and whitening effects by organically combining ingredients that provide an antioxidant and restorative environment with ingredients involved in regulating skin structure and pigmentation.

[0017] The composition according to the present invention includes melatonin as a component involved in the nocturnal recovery environment and antioxidant action of skin cells, includes resveratrol which alleviates skin damage caused by photoaging through the removal of free radicals, and includes bakuchiol which contributes to wrinkle improvement by regulating collagen metabolism through retinol-like action. In addition, the present invention relates to the giant water lily (which has a protective function against external environmental stress due to the characteristics of plants surviving in aquatic environments). Victoria Amazonica By including additional ingredients derived from ), the aim was to complement the skin structure stabilization and pigmentation improvement effects, which are difficult to sufficiently secure with only the basic active ingredients mentioned above.

[0018] Accordingly, the present invention relates to melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica Provides a functional cosmetic composition for wrinkle improvement and whitening containing an extract.

[0019] In the above composition, it is preferable that each component be included in the following content ranges based on the total weight of the composition.

[0020] It is preferable that melatonin be included in an amount of 0.00001% to 0.01% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.00001% by weight, is intended to secure a minimum effective content capable of mitigating cell damage caused by photoaging and inhibiting oxidative stress pathways associated with wrinkle formation through the creation of a nocturnal recovery environment for skin cells and antioxidant action. At a lower content than this, the wrinkle improvement and skin condition recovery effects expected as a functional cosmetic may not be sufficiently manifested. Meanwhile, the upper limit of the above content range, 0.01% by weight, is a limit considering stability and formulation suitability when applied to cosmetics; if this is exceeded, there is a concern that formulation stability may be reduced or efficiency may decrease due to unnecessary over-injection.

[0021] It is preferable that Resveratrol be included in an amount of 0.001% to 1.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.001% by weight, is the minimum effective amount for inhibiting UV-induced photoaging and melanin production-promoting pathways by exhibiting an antioxidant effect through the scavenging of free radicals. At a content lower than this, it is difficult to sufficiently demonstrate synergistic antioxidant effects related to wrinkle improvement and whitening. The upper limit of the above content range, 1.0% by weight, is a range considering solubility, formulation stability, and skin applicability; exceeding this may result in formulation issues or a decrease in usability.

[0022] It is preferable that Bakuchiol be included in an amount of 0.01% to 2.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.01% by weight, is the minimum effective amount for regulating collagen metabolism and exhibiting a wrinkle-improving effect through retinol-like action. The upper limit of the above content range, 2.0% by weight, is a range that takes into account stability in the cosmetic formulation and the possibility of skin irritation; if this is exceeded, formulation stability or sensory quality may be degraded due to excessive addition.

[0023] The large water lily used in the present invention ( Victoria Amazonica It is desirable that the extract be manufactured to efficiently recover active ingredients that can contribute to skin protection against external environmental stress and skin structure stabilization while maintaining the physiological characteristics of the aquatic plant. The above-mentioned giant water lily ( Victoria Amazonica The extract can be obtained from leaves, flowers, or a mixture thereof, and the method of preparation may include the following steps.

[0024] First of all, the giant water lily ( Victoria Amazonica After harvesting the leaves and / or flowers of ), they are washed with purified water to remove foreign substances, and if necessary, naturally dried or low-temperature dried to obtain a dried product. It is preferable to grind the dried product into a powder state to improve extraction efficiency.

[0025] Subsequently, the powder or raw material in a biological state is immersed in an extraction solvent comprising one or more of purified water, ethanol, or a mixed solvent of purified water and ethanol to perform extraction. At this time, considering skin applicability and the stability of the active ingredient, the extraction solvent is preferably an aqueous ethanol solution of 30 to 80 weight%, and the extraction temperature can be selected from room temperature to 70°C, and the extraction time can be selected from 2 to 48 hours.

[0026] After extraction is completed, the extract is filtered or centrifuged to remove solids, and if necessary, vacuum concentration is performed to obtain an extract concentrated with active ingredients. The concentrated extract may also be prepared in powder form through freeze-drying or spray-drying processes to improve formulation stability and shelf life.

[0027] In addition, the above large water lily ( Victoria Amazonica The extract may additionally include filtration, sterilization, or pH adjustment processes to improve skin applicability, and may also be provided dissolved in a cosmetic solvent such as glycerin, butylene glycol, or propanediol as needed.

[0028] Large water lily prepared by the above method ( Victoria Amazonica The extract derived from ) contains ingredients that protect the skin from external stimuli and contribute to the alleviation of skin stress related to photoaging and pigmentation, due to the characteristics of plants surviving in an aquatic environment, and can be applied as an active ingredient in the functional cosmetic composition for wrinkle improvement and whitening of the present invention.

[0029] Large water lily ( Victoria Amazonica It is preferable that the extract be included in an amount of 0.01% to 5.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.01% by weight, is the minimum effective amount for exhibiting skin protection, wrinkle improvement, and whitening effects attributable to the characteristics of the aquatic plant-derived ingredients. The upper limit of the above content range, 5.0% by weight, is a range considering the physical properties, stability, and sensory quality of the formulation; exceeding this may result in increased viscosity or formulation instability.

[0030] In addition, the functional cosmetic composition for wrinkle improvement and whitening according to the present invention preferably further includes Dragon's Blood extract, a resin component derived from plants, to protect the skin from external environmental stress associated with skin aging and to assist in stabilizing skin structure and alleviating pigmentation. By forming a protective film on the skin surface or assisting in the skin's protective function against the external environment, Dragon's Blood extract can contribute to alleviating skin damage caused by ultraviolet rays and oxidative stress, and to inhibit skin stress pathways associated with wrinkle formation and melanin accumulation. That is, by additionally including Dragon's Blood, the composition of the present invention can maintain the effects of skin structure stabilization and skin tone uniformization more stably over time, going beyond the temporary skin improvement effect immediately after application.

[0031] The Dragon's Blood extract used in the present invention is preferably prepared using resin naturally secreted from plants as a raw material. The Dragon's Blood may be obtained by collecting resin exuded from the stems or branches of plants of the genus Dracaena.

[0032] Specifically, after collecting the Dragon's Blood resin, a sieving or simple washing process is performed to remove foreign substances. Subsequently, it is desirable to grind the resin or prepare it in the form of small pieces to increase extraction efficiency.

[0033] The prepared resin above may be extracted by immersing it in an extraction solvent comprising one or more of purified water, ethanol, or a mixed solvent of purified water and ethanol. At this time, considering the solubility and stability of the resin components, it is preferable to use an aqueous ethanol solution of 50 to 95 weight%, and the extraction temperature may be selected from room temperature to 60°C, and the extraction time may be selected from 1 to 48 hours.

[0034] After extraction is completed, the extract is filtered or centrifuged to remove insoluble solids, and if necessary, concentrated under reduced pressure to obtain the dragon's blood extract. The extract may be prepared in powder form through freeze-drying or spray-drying processes to improve formulation stability and shelf life.

[0035] In addition, the above dragon's blood extract may further include a process of sterilization, decolorization, pH adjustment, or redissolution in a cosmetic solvent such as glycerin or butylene glycol to improve skin applicability.

[0036] The dragon's blood-derived extract prepared by the above method is a resin component derived from a plant, which acts as a protective barrier on the skin surface to shield it from external stimuli, and can contribute to inhibiting wrinkle formation and improving pigmentation by alleviating skin damage associated with oxidative stress and photoaging.

[0037] In the functional cosmetic composition for wrinkle improvement and whitening according to the present invention, it is preferable that the dragon's blood-derived extract be included in an amount of 0.001% by weight to 3.0% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.001% by weight, represents the minimum effective content for the dragon's blood resin component to form a protective film on the skin surface and protect the skin from external environmental stress, thereby exhibiting the effect of alleviating skin damage associated with wrinkles and pigmentation. At a content lower than this, the skin protection, wrinkle, and whitening auxiliary effects may not be sufficiently expressed. The upper limit of the above content range, 3.0% by weight, is a range considering stability, usability, and color influence in the cosmetic formulation; exceeding this range may lead to an increase in the viscosity of the formulation, a change in color, or a deterioration in sensory quality. Furthermore, the inclusion of an excessive amount of the resin component may reduce the efficiency relative to the wrinkle improvement and whitening effects.

[0038] Furthermore, the functional cosmetic composition for wrinkle improvement and whitening according to the present invention preferably further comprises Nonapeptide-1 to more effectively improve wrinkle formation and skin tone deterioration caused by skin aging. By assisting in the energy metabolic activity and maintenance of cell function of skin cells, Nonapeptide-1 can improve skin condition deteriorated by aging or external environmental stress, and contribute to alleviating wrinkle formation and skin tone unevenness caused by the deterioration of skin cell function. Additionally, when Nonapeptide-1 is used in combination with melatonin and resveratrol, the effect of alleviating oxidative stress is enhanced by the skin cell recovery environment and antioxidant synergy, thereby promoting the manifestation of wrinkle improvement and whitening effects more stably. Therefore, the additional inclusion of Nonapeptide-1 can contribute to the composition of the present invention going beyond simple temporary skin improvement to enhance the effect of maintaining skin condition from the perspective of wrinkle improvement and whitening.

[0039] The Nonapeptide-1 used in the present invention is a peptide composed of nine amino acids and can be provided in the form of a synthetic peptide commercially available as a cosmetic ingredient. The Nonapeptide-1 can generally be produced by solid-phase peptide synthesis (SPPS) and can be provided as a commercial ingredient in a state dissolved in an aqueous solution, glycerin, or butylene glycol.

[0040] Specifically, Nonapeptide-1 can be obtained in powder or solution form from a company specializing in peptide synthesis, and can be applied to cosmetic formulations after undergoing sterilization, filtration, or dilution processes as needed. Since Nonapeptide-1 in this form has ensured stability and reproducibility as a cosmetic ingredient, it is suitable for application in the functional cosmetic composition for wrinkle improvement and whitening according to the present invention.

[0041] In the functional cosmetic composition for wrinkle improvement and whitening according to the present invention, it is preferable that Nonapeptide-1 be included in an amount of 0.0001% to 0.05% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.0001% by weight, represents the minimum effective content required to exhibit the effect of alleviating skin aging phenomena associated with wrinkle formation and skin tone deterioration by assisting in the energy metabolic activity and maintenance of cellular function of skin cells. At a content lower than this, the antioxidant and restorative environment-creating effects resulting from co-administration with melatonin and resveratrol may not be sufficiently expressed. The upper limit of the above content range, 0.05% by weight, is a range that considers stability in the cosmetic formulation, cost-efficiency, and the benefits of excessive addition based on the peptide characteristics; exceeding this may result in reduced formulation stability or decreased efficiency relative to wrinkle improvement and whitening effects. Furthermore, excessive peptide content may lead to unnecessary constraints in terms of formulation design.

[0042] In addition, the functional cosmetic composition for wrinkle improvement and whitening according to the present invention preferably further includes Tripeptide-72 to more effectively alleviate wrinkle formation and weakening of skin structure caused by skin aging. Tripeptide-72 participates in collagen metabolism, which is closely related to maintaining skin structure, and acts to promote collagen synthesis or inhibit collagen degradation pathways; thereby, it can contribute to supporting the structural support of the skin and alleviating the decrease in elasticity associated with wrinkle formation. In particular, when Tripeptide-72 is used in combination with bakuchiol, the collagen metabolism regulation and skin structure stabilization effects act complementarily, which can further enhance the manifestation and duration of the wrinkle improvement effect.

[0043] The tripeptide-72 used in the present invention is a peptide composed of three amino acids and can be provided in the form of a synthetic peptide commercially available as a cosmetic ingredient. The tripeptide-72 is generally manufactured by solid-phase peptide synthesis (SPPS) and can be provided in the form of a powder or solution after undergoing a purification process following manufacturing.

[0044] Specifically, Tripeptide-72 can be obtained as a high-purity peptide raw material from a company specializing in peptide synthesis, and, if necessary, can be provided dissolved in a cosmetic solvent such as purified water, glycerin, butylene glycol, or propanediol. Tripeptide-72 in this form has excellent formulation stability and reproducibility, making it suitable for application in the functional cosmetic composition for wrinkle improvement and whitening according to the present invention.

[0045] In the functional cosmetic composition for wrinkle improvement and whitening according to the present invention, it is preferable that tripeptide-72 be included in an amount of 0.0001% by weight to 0.1% by weight relative to the total weight of the composition. The lower limit of the above content range, 0.0001% by weight, represents the minimum effective content for tripeptide-72 to participate in collagen metabolism to exhibit skin structure stabilization and wrinkle improvement effects. At a content lower than this, the effects of inhibiting wrinkle formation and improving skin structural support may not be sufficiently expressed. The upper limit of the above content range, 0.1% by weight, is a range considering stability, cost-efficiency, and peptide characteristics in the cosmetic formulation; exceeding this may result in reduced efficiency relative to effect or cause unnecessary constraints in formulation design. Furthermore, an excessive peptide content may affect formulation stability or sensory quality.

[0046] The above components of the present invention can be used as a functional cosmetic composition for wrinkle improvement and whitening effects.

[0047] The above-mentioned component of the cosmetic composition of the present invention may be included in an amount of 0.01 to 50% by weight based on the total weight of the composition, preferably 0.1 to 20% by weight, and more preferably 1 to 10% by weight. Within this content range, appropriate formulation stability can be secured and the desired effect can be expected.

[0048] In addition to the above-mentioned components of the present invention, the composition of the present invention may be used by additionally including known natural extracts or other components for the effects of antioxidant, anti-aging, and skin regeneration promotion, to the extent that such effects are not inhibited.

[0049] In addition, the above composition may be formed into a specific formulation by including auxiliary agents or carriers commonly used in the fields of cosmetics or dermatology, such as fatty substances, organic solvents, solvents, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion chelating agents and chelating agents, preservatives, vitamins, blockers, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents.

[0050] The cosmetic composition of the present invention can be prepared in any formulation conventionally manufactured in the art, for example, as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, pack, soap, surfactant-containing cleansing, oil, and spray, but is not limited thereto. More specifically, it can be prepared in formulations such as a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, a powder, a compact, a lip gloss, a lipstick, an eyeshadow, a shampoo, and a rinse.

[0051] In the case where the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0052] In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0053] When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0054] In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used as carrier components.

[0055] In the case where the formulation of the present invention is a cleansing agent containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0056] The present invention will be explained in more detail below through specific embodiments. The following embodiments describe a preferred embodiment of the present invention, and the scope of the present invention is not to be interpreted as being limited by the matters described in the following embodiments.

[0057] [Example]

[0058] Example 1

[0059] Melatonin, Resveratrol, Bakuchiol, and giant water lily ( Victoria Amazonica A functional cosmetic composition containing ) extract was prepared.

[0060] Specifically, an aqueous phase containing purified water, glycerin, and butylene glycol was prepared and stirred while heating it to approximately 70°C. Separately, an oil phase containing vegetable oil, fatty acid ester, and an emulsifier was heated to the same temperature, and then the oil phase was added to the aqueous phase while high-speed stirring or homogenization was performed to form an emulsion.

[0061] Subsequently, the formed emulsion was slowly cooled while continuously stirring to obtain a semi-solid composition with increased viscosity, and during the cooling process, in a temperature range of 40°C or lower, melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria AmazonicaThe extracts were added sequentially and stirred sufficiently to disperse them uniformly. Afterwards, thickeners, stabilizers, or texture improvers were added as needed to adjust the viscosity and spreadability suitable for a cream formulation.

[0062] Finally, the pH of the entire composition was adjusted to a range suitable for the skin, and then air was removed through a degassing process and filled into a container to complete the functional cosmetic composition in the form of a cream.

[0063] Example 2

[0064] A functional cosmetic composition was prepared in the same manner as in Example 1, but the composition additionally included Dragon's Blood extract.

[0065] Specifically, an aqueous phase containing purified water, glycerin, and butylene glycol was prepared and stirred while heating to around 70°C, and separately an oil phase containing vegetable oil, fatty acid ester, and an emulsifier was heated to the same temperature, and then high-speed stirring or homogenization was performed while adding the oil phase to the aqueous phase to form an emulsion.

[0066] Subsequently, the formed emulsion is slowly cooled while continuously stirring, and during the cooling process, in a temperature range of 40℃ or lower, melatonin, resveratrol, bakuchiol, giant water lily ( Victoria Amazonica Dragon's blood extract was added sequentially along with the extract and uniformly dispersed. Then, a thickening agent, a stabilizer, or a texture improver was added as needed to adjust the viscosity and spreadability suitable for a cream formulation, and a functional cosmetic composition in a cream formulation was completed by filling it into a container after pH adjustment and degassing processes.

[0067] Example 3

[0068] A functional cosmetic composition was prepared in the same manner as in Example 2, but the composition additionally included Nonapeptide-1.

[0069] Specifically, after preparing the aqueous and oil phases separately and performing an emulsification process at the same temperature, while cooling the emulsion in a temperature range of 40℃ or lower, melatonin, resveratrol, bakuchiol, giant water lily ( Victoria Amazonica ) extract and Dragon's Blood extract were added.

[0070] Subsequently, during the final cooling step, Nonapeptide-1 was added in a state of pre-dissolution in purified water or butylene glycol and stirred sufficiently to uniformly disperse it within the composition. Then, thickeners and texture improvers were selectively added to adjust physical properties suitable for a cream formulation, and a functional cosmetic composition in the form of a cream was completed through pH adjustment and degassing processes.

[0071] Example 4

[0072] A functional cosmetic composition was prepared in the same manner as in Example 3, but the composition additionally included Tripeptide-72.

[0073] Specifically, through emulsification and cooling processes, melatonin, resveratrol, bakuchiol, and giant water lily ( Victoria Amazonica After adding ) extract, dragon's blood extract and nonapeptide-1, tripeptide-72 was additionally added during the final cooling step and stirred sufficiently to maintain a uniform dispersion state.

[0074] Subsequently, a thickening agent or a stabilizer was optionally added to control the viscosity and spreadability of the composition, and a pH adjustment and degassing process was performed before filling the composition into a container to complete a functional cosmetic composition in the form of a cream.

[0075] Comparative Example 1

[0076] A commercially available general whitening and anti-wrinkle cream cosmetic from Company C was used. It contains arbutin and niacinamide as main ingredients.

[0077] Comparative Example 2

[0078] A functional cosmetic composition containing only melatonin, resveratrol, and bakuchiol was prepared.

[0079] The manufacturing method was the same as in Example 1, in which an aqueous phase and an oil phase were prepared separately and an emulsification process was performed; after adding melatonin, resveratrol, and bakuchiol at a temperature of 40°C or lower during the cooling process, a cream-form cosmetic composition was completed through viscosity adjustment, pH adjustment, and degassing processes. However, the above composition contains water lily ( Victoria Amazonica It did not contain ) extract, Dragon's Blood, or peptides.

[0080] Experimental Example 1. Evaluation of Collagen Synthesis Increase and MMP-1 Inhibitory Effects

[0081] In this experimental example, an in vitro test using human dermal fibroblasts was performed to evaluate the effect of the composition of the present invention on collagen metabolism related to wrinkle improvement. After culturing human dermal fibroblasts under standard culture conditions, the compositions of each example and comparative example were diluted in the culture medium and treated to the cells. The sample treatment on the cells was performed within a concentration range that did not exhibit cytotoxicity, and culture was continued for a certain period after treatment. Subsequently, the cell culture supernatant was collected, and the expression levels of Procollagen type I C-peptide (PIP), an indicator of collagen synthesis, and MMP-1, an enzyme related to collagen degradation, were quantified using an enzyme-linked immunosorbent assay (ELISA). Each measurement was repeated under the same conditions to calculate the average value, and the results were compared as the rate of change relative to the control group.

[0082] division Procollagen MMP-1 Example 1 28 31 Example 2 33 38 Example 3 39 45 Example 4 47 53 Comparative Example 1 5 6 Comparative Example 2 18 22

[0083] As shown in Table 1 above, the composition of the example significantly increased the amount of procollagen produced compared to the comparative example and showed a tendency to effectively inhibit MMP-1 expression, thereby exhibiting a collagen metabolism regulating effect favorable for wrinkle improvement.

[0084] Experimental Example 2. Evaluation of wrinkle improvement effect applied to the human body

[0085] In this experimental example, a human application test was conducted to evaluate whether the composition of the present invention exhibits a wrinkle-improving effect on actual skin. Healthy adult subjects were selected, and the test area was set to the eye area or a facial area where wrinkles are observed. A specific amount of each example and comparative example composition was repeatedly applied over a certain period. The wrinkle depth and roughness indices of the skin surface were measured using a 3D skin analysis device at a reference point before application and after a certain period had elapsed following application. Measurements were repeated on the same area under the same conditions to calculate the average value, and the rate of change compared to before application was compared.

[0086] division Improvement rate (%) of wrinkle depth and roughness indicators Example 1 12 Example 2 15 Example 3 19 Example 4 24 Comparative Example 1 2 Comparative Example 2 7

[0087] As shown in Table 2 above, when the composition of the example was applied, the wrinkle depth and skin roughness indices showed a tendency to be significantly improved compared to the comparative example, confirming that the composition of the present invention effectively provides a wrinkle improvement effect when applied to the human body.

[0088] Experimental Example 3. Evaluation of Tyrosinase Inhibition and Melanin Production Inhibition Effects

[0089] In this experimental example, tyrosinase inhibition and melanin production inhibition tests were performed to evaluate the effect of the composition of the present invention on the melanin production pathway related to skin whitening. First, the tyrosinase activity inhibition rate was calculated by measuring the change in absorbance according to the substrate reaction after treating each example and comparative example composition using a tyrosinase enzyme reaction system. In addition, to evaluate the melanin production inhibition effect, melanoma cells were cultured to induce melanin production, and after treating each sample and allowing it to react for a certain period of time, the intracellular melanin content was quantified. Each test was repeated under the same conditions to calculate the average value, and the melanin production inhibition rate was compared with the control group.

[0090] division Tyrosinase Melanin Example 1 22 26 Example 2 25 29 Example 3 29 33 Example 4 32 36 Comparative Example 1 4 5 Comparative Example 2 15 18

[0091] As shown in Table 3 above, the composition of the example effectively inhibited tyrosinase activity and showed a tendency to significantly reduce melanin production compared to the comparative example, confirming that it exhibited activity favorable for whitening effects.

[0092] Experimental Example 4. Evaluation of whitening effect applied to the human body

[0093] In this experimental example, a human application test was conducted to evaluate whether the composition of the present invention exhibits a whitening effect on human skin. Healthy adult subjects were selected, and the test area was set to the cheek or forearm. The compositions of each example and comparative example were repeatedly applied for a certain period. The skin's melanin index was measured using a melanin index measuring device at a reference point before application and after a certain period had elapsed following application. Repeated measurements were taken on the same area to calculate the average value.

[0094] division MI Reduction Rate (%) Example 1 7 Example 2 8 Example 3 10 Example 4 12 Comparative Example 1 1 Comparative Example 2 4

[0095] As shown in Table 4 above, when the reduction rate of the melanin index was calculated and compared compared to before application, it was found that when the composition of the example was applied, the melanin index tended to decrease more significantly than in the comparative example, confirming that the composition of the present invention provides an excellent whitening effect when applied to the human body.

Claims

Claim 1 Melatonin, Resveratrol, Bakuchiol, giant water lily ( Victoria Amazonica A cosmetic composition for wrinkle improvement and whitening functions comprising ) extract, Dragon's Blood extract, Nonapeptide-1, and Tripeptide-72. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete

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