Pest repellent composition containing natural raw material complex extract and method for producing same

Abstract

The present invention relates to an insect repellent composition comprising a natural raw material extract and a method for manufacturing the same. The composition of the present invention is harmless to the human body and can exhibit an excellent insect repellent effect.

Description

Technology Field

[0001] The present invention relates to an insect repellent composition comprising a complex extract of natural raw materials and a method for manufacturing the same. Background Technology

[0002] As ecosystems change due to the recent impacts of climate change and urbanization, the habitats of pests that previously appeared only in specific regions or times are expanding, and their active periods are also becoming longer.

[0003] The increase in various pests, including insects, flies, and mites, as well as love bugs and mayflies, which have appeared in large numbers in recent years and caused serious inconvenience, particularly during the hot and humid summer season, causes significant discomfort in daily human life. Furthermore, these pests can act as vectors for fatal infectious diseases such as malaria, Japanese encephalitis, dengue fever, and severe fever with thrombocytopenia syndrome (SFTS), posing a serious threat to public hygiene and public health.

[0004] Accordingly, the importance of developing insect repellents to effectively block or deter these pests is continuously being highlighted.

[0005] Currently available or researched insect repellents can be broadly classified into those based on chemically synthesized ingredients and those based on naturally derived ingredients, depending on their main components.

[0006] Representative conventional chemical repellent ingredients include diethyltoluamide (DEET), picaridin, and ethylbutylacetylaminopropionate (IR3535). These chemically synthesized ingredients have the distinct advantages of excellent repellent efficacy against pests and a long duration.

[0007] However, using it at high concentrations or applying it repeatedly to the skin may cause skin irritation, rashes, and allergic reactions, and potential concerns regarding the neurotoxicity of some ingredients have been raised. Consequently, its use is restricted for infants and users with sensitive skin, and its distinctive chemical odor and the potential to damage plastics or synthetic fibers are also cited as drawbacks.

[0008] As an alternative to address the safety issues of the aforementioned chemical repellents, research on natural repellents utilizing natural plant-derived extracts or essential oils, which are relatively safe for the human body and environmentally friendly, has been actively conducted.

[0009] Ingredients derived from citronella, lemon eucalyptus, lavender, peppermint, cinnamon, etc., are known to have a repellent effect against certain pests.

[0010] However, most of these single-natural ingredient-based repellents are highly volatile, so the repellent component evaporates easily into the air, and the duration of the repellent effect is very short, lasting less than 2 hours.

[0011] In addition, there is a problem in that it is difficult to achieve satisfactory results due to the relatively low repellent efficacy compared to chemical components, and it is difficult to respond to various pests simultaneously because the spectrum of repellent activity is often narrow, such as being limited to specific pests.

[0012] Therefore, there is a very high technical demand for the development of a new concept of insect repellent that ensures high safety for the human body, exhibits strong and sustained repellent efficacy comparable to chemical repellents, and furthermore possesses broad repellent activity against various pests that are problematic in modern times, such as pests and love bugs. The problem to be solved

[0013] To solve the above-mentioned problems, the present invention aims to provide a composition containing a specific natural raw material complex extract as an active ingredient that is harmless to the human body and can exhibit an excellent insect repellent effect.

[0014] The present invention relates to an insect repellent composition comprising extracts of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, *Apple mint* leaf extract, *Actinidia polygama* extract, and *Artemisia annua* extract.

[0015] The present invention relates to an insect repellent composition comprising, for every 100 parts by weight of the Chrysanthemum zawadskii extract, 80 to 120 parts by weight of the catnip extract, 80 to 120 parts by weight of the shepherd's purse extract, 80 to 120 parts by weight of the apple mint leaf extract, 80 to 120 parts by weight of the Actinidia polygala extract, and 80 to 120 parts by weight of Artemisia annua extract.

[0016] The present invention relates to an insect repellent composition in which an extract of *Chrysanthemum zawadskii*, an extract of *Cat's-eye*, an extract of *Shepherd's purse*, or an extract of *Apple mint* leaves is prepared by a method comprising the following steps:

[0017] S1) 70 to 80 leaves of mountain aster, wild mint, shepherd's purse, or apple mint o Steaming step at a temperature of C;

[0018] S2) 60 to 80 of the above steamed *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, or apple mint leaves o A step of primary drying at a temperature of C for 1 to 3 hours;

[0019] S3) 50 to 60 of the above primary dried *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves o A step of secondary drying at a temperature of C for 5 to 10 hours;

[0020] S4) Dried *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves -50 to -40 o Step of freezing at a temperature of C;

[0021] S5) A step of aging the above-mentioned frozen *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves at room temperature;

[0022] S6) A step of crushing the aged *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves;

[0023] S7) The crushed mountain aster, catnip, shepherd's purse, or apple mint leaves are added to a solvent, and 50 to 60 o Step of extracting by reflux cooling at a temperature of C; and

[0024] S8) A step of filtering and concentrating the above extract.

[0025] The present invention relates to a method for manufacturing the above-mentioned insect repellent composition. means of solving the problem

[0026] The following describes this in detail. All combinations of the various elements disclosed in this invention fall within the scope of this invention. Furthermore, the scope of this invention should not be considered limited by the following detailed description.

[0027] Furthermore, unless otherwise defined in this specification, all terms used herein should be understood to have the same meaning as generally understood by those skilled in the art to which the present invention pertains.

[0028] In addition, singular expressions include plural expressions unless they are interpreted as being distinct from the context.

[0029] In this specification, terms such as "first," "second," etc., may be used to distinguish or describe one component from another, but the components should not be interpreted restrictively by said terms. For example, within the scope of the present invention, a first component may be named as a second component or vice versa.

[0030] In addition, "degree" combined with a number in this specification refers to degrees Celsius. oRepresenting C), " o It can be used interchangeably with "C" (for example, "100 degrees" is "100 o (Used with the same meaning as C).

[0032] The present invention provides an insect repellent composition comprising extracts of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, *Apple mint*, *Actinidia polygama*, and *Artemisia annua*.

[0033] In the present invention, "Chrysanthemum zawadskii var. latilobum" is a perennial dicotyledonous plant belonging to the Asteraceae family. Chrysanthemum zawadskii grows wild in mountainous and high-altitude areas throughout the country and also grows in China, Russia, Mongolia, Japan, and other regions. Chrysanthemum zawadskii is used as a folk remedy for amenorrhea, pneumonia, bronchitis, the common cold, pharyngitis, etc.

[0034] In this invention, "catnip (Nepeta cataria)" is a perennial plant belonging to the Lamiaceae family, widely distributed in Europe, Asia, and North America, and can also be easily found in Korea. Catnip is known as a plant that cats like due to its distinctive scent, and it has traditionally been used as a folk remedy for sedation, indigestion, colds, and insomnia.

[0035] According to embodiments of the present invention, the catnip extract may be included in an amount of 80 to 120 parts by weight per 100 parts by weight of the *Chrysanthemum zawadskii* extract, but is not limited thereto.

[0036] In this invention, "shepherd's purse (Capsella bursa-pastoris)" is a biennial plant belonging to the Brassicaceae family, distributed in temperate regions worldwide, and commonly grows in fields or farms in Korea. Shepherd's purse is rich in vitamins and minerals and is enjoyed as a spring vegetable; in traditional Korean medicine, it is known to have hemostatic, diuretic, and detoxifying effects and is used for digestive or hemorrhagic diseases.

[0037] According to embodiments of the present invention, the shepherd's purse extract may be included in an amount of 80 to 120 parts by weight per 100 parts by weight of the mountain daisy extract, but is not limited thereto.

[0038] In this invention, "Apple mint (Mentha suaveolens)" is a perennial plant belonging to the Lamiaceae family, native to Southern Europe, and cultivated worldwide. It is characterized by a refreshing apple scent and is used in various ways, such as in tea, cooking, and as a spice. Apple mint leaves are known to help promote digestion, alleviate headaches, and relieve stress.

[0039] According to embodiments of the present invention, the apple mint leaf extract may be included in an amount of 80 to 120 parts by weight per 100 parts by weight of the Angelica gigas extract, but is not limited thereto.

[0040] In this invention, "Actinidia polygama" is a deciduous vine belonging to the Actinidiaceae family, mainly distributed in mountainous areas of East Asia, such as Korea, Japan, and China. Although the fruit of Actinidia polygama resembles that of the kiwi, it tastes sour, and the galls formed by parasitic insects are widely used as a medicinal ingredient. It has been used as a folk remedy for gout, neuralgia, arthritis, etc.

[0041] According to embodiments of the present invention, the Actinidia polygama extract may be included in an amount of 80 to 120 parts by weight per 100 parts by weight of the Chrysanthemum zawadskii extract, but is not limited thereto.

[0042] In this invention, "Artemisia annua" is an annual plant belonging to the Asteraceae family. It is widely distributed globally and is commonly found in fields and along roadsides in Korea. Artemisia annua is attracting attention for its distinctive strong scent and for containing artemisinin, a component traditionally used as a treatment for malaria. Active research is currently underway regarding its efficacy in reducing fever, inflammation, and cancer.

[0043] According to embodiments of the present invention, the Artemisia annua extract may be included in an amount of 80 to 120 parts by weight per 100 parts by weight of the Angelica dahurica extract, but is not limited thereto.

[0044] In the present invention, "extract" refers to a product obtained by extraction using a suitable extraction solvent, and said extract includes the extract itself and all formulations of extracts that can be formed using the extract, such as an extract obtained by extraction, a diluted or concentrated extract, a dried product obtained by drying the extract, a modified or purified product thereof, a fraction thereof, or a mixture thereof.

[0045] The extract of the present invention may be prepared and used in the form of a dried powder after extraction, but is not limited thereto.

[0046] In the present invention, the extract may be prepared using extraction methods commonly used in the art. For example, methods such as hot water extraction, hot water extraction, cold maceration extraction, reflux cooling extraction, ultrasonic extraction, and high-temperature pressurized extraction may be used.

[0047] In preparing the extract of the present invention, the extraction solvent may be water; lower alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, propyl alcohol, butyl alcohol; polyalcohols, such as glycerin, butylene glycol, propylene glycol; hydrocarbon solvents, such as chloroform, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, petroleum ether, dichloromethane; or a mixture thereof, but is not limited thereto.

[0048] In the present invention, the extract of *Chrysanthemum zawadskii*, extract of *Cat's-eye*, extract of *Shepherd's purse*, or extract of *Apple mint* may be prepared by a method comprising the following steps:

[0049] S1) 70 to 80 leaves of mountain aster, wild mint, shepherd's purse, or apple mint o Steaming step at a temperature of C;

[0050] S2) 60 to 80 of the above steamed *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, or apple mint leaves o A step of primary drying at a temperature of C for 1 to 3 hours;

[0051] S3) 50 to 60 of the above primary dried *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves o A step of secondary drying at a temperature of C for 5 to 10 hours;

[0052] S4) Dried *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves -50 to -40 o Step of freezing at a temperature of C;

[0053] S5) A step of aging the above-mentioned frozen *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves at room temperature;

[0054] S6) A step of crushing the aged *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves;

[0055] S7) The crushed mountain aster, catnip, shepherd's purse, or apple mint leaves are added to a solvent, and 50 to 60 o Step of extracting by reflux cooling at a temperature of C; and

[0056] S8) A step of filtering and concentrating the above extract.

[0057] In the present invention, steps S1 to S5 are a type of pretreatment step, performed to increase the activity of the components contained in the raw material and to increase the extraction yield of the active components in subsequent steps, thereby maximizing the intended effect of the present invention.

[0058] In the present invention, the S1 step is a step of steaming raw materials, 70 to 80 o It is performed at a temperature of C. If performed at a temperature lower than the above temperature range, the increase in effect obtained from pre-treating the raw material may be negligible, and if performed at a temperature exceeding the above range, destruction or denaturation of the active ingredient may occur, which may be undesirable.

[0059] In addition, the above S1 step may be performed for 1 to 15 minutes, preferably 5 to 15 minutes, but is not limited thereto.

[0060] If performed for less than the above time range, the increase in effect obtainable from pre-treating the raw material may be negligible, and if performed for more than the above range, destruction or denaturation of the active ingredient may occur, which may be undesirable.

[0061] In the present invention, the S2 and S3 steps are steps for drying the steamed raw material, and are performed by dividing them into first and second drying steps with different conditions.

[0062] The first drying in the above S2 step is 60 to 80 o It is performed at a temperature of C for 1 to 3 hours, and the secondary drying of step S3 is 50 to 60 o It is performed for 5 to 10 hours at a temperature of C.

[0063] If drying, which is performed through separate steps as described above, is carried out outside the respective temperature and time ranges, the increase in effect obtained by performing pretreatment in the present invention may be negligible, or problems such as destruction or denaturation of the active ingredient may occur.

[0064] In the present invention, the S4 step is a step of freezing the dried raw material, with a temperature of -50 to -40 o It is performed at a temperature of C. According to an embodiment of the present invention, the S4 step may be performed using equipment capable of rapid freezing, and the freezing may be performed by maintaining it for 3 to 6 hours, but is not limited thereto.

[0065] In the present invention, the above step S5 is a step of thawing the frozen raw material at room temperature and performing aging, which is performed to control the ratio of the active ingredient exhibiting a specific effect by controlling the content (content ratio) of the active ingredient.

[0066] According to an embodiment of the present invention, the aging in step S5 is performed at room temperature (20±5 o It may be performed in C), and may also be performed for 1 to 10 hours, preferably 5 to 10 hours, more preferably 5 to 7 hours, but is not limited thereto.

[0067] In the present invention, the above-mentioned step S6 is a step of crushing raw materials to increase extraction efficiency, and can be performed through a method commonly used in the industry.

[0068] In the present invention, the step S7 is a step of preparing an extract from raw materials, and according to an embodiment of the present invention, the solvent may be water, but is not limited thereto.

[0069] In the above S7 step, the extraction is 50 to 60 oIt is performed at a temperature of C. If performed at a temperature below the above range, the extraction of the active ingredient may not be sufficient, and the effect intended by the present invention may be negligible. If performed at a temperature exceeding the above range, destruction or denaturation of the active ingredient may occur, and it may not be suitable.

[0070] In addition, the extraction in step S7 above may be performed for 3 to 10 hours, preferably for 3 to 7 hours, but is not limited thereto. If performed for a time shorter than the above range, the extraction of the active ingredient may not be sufficient, and the effect intended by the present invention may be negligible; if performed for a time longer than the above range, destruction or denaturation of the active ingredient may occur, and it may not be appropriate.

[0071] In the present invention, the step S8 is a step of filtering and concentrating the extract obtained in step S7, and the method is not limited and can be performed through methods commonly used in the art.

[0072] In the present invention, the Actinidia polygala extract and / or Artemisia annua extract may be prepared by a method comprising the following steps:

[0073] G1) Step of crushing Actinidia polygama and / or Artemisia annua;

[0074] G2) A step of adding a solvent to the ground Actinidia polygala and / or Artemisia annua and performing reflux cooling extraction; and

[0075] G3) A step of filtering and concentrating the above extract.

[0076] In the present invention, the G1 step is performed to increase extraction efficiency by increasing the surface area of ​​the raw material, and can be performed through methods commonly known in the art.

[0077] In the present invention, the G2 step is a step of performing extraction from raw materials.

[0078] According to an embodiment of the present invention, the solvent may be 70% (v / v) ethanol, but is not limited thereto.

[0079] According to an embodiment of the present invention, the G2 step may be performed for 1 to 5 hours, preferably 1 to 3 hours, but is not limited thereto.

[0080] According to an embodiment of the present invention, the sugarcane, Schisandra, peony and / or Cnidium root extract may be extracted by reflux cooling for 1 to 5 hours using 70% (v / v) ethanol as a solvent, but is not limited thereto.

[0081] In the present invention, the G3 step is a step of filtering and concentrating the Actinidia polygala extract and / or Artemisia annua extract, and can be performed through methods commonly used in the art.

[0082] According to embodiments of the present invention, the insect repellent composition may comprise, with respect to 100 parts by weight of the *Chrysanthemum zawadskii* extract, 80 to 120 parts by weight of *Catnip* extract, 80 to 120 parts by weight of *Shepherd's Purse* extract, 80 to 120 parts by weight of *Apple Mint* leaf extract, 80 to 120 parts by weight of *Actinidia polygama* extract, and 80 to 120 parts by weight of *Artemisia annua* extract, but is not limited thereto.

[0083] According to one embodiment of the present invention, the insect repellent composition may comprise, with respect to 100 parts by weight of the *Chrysanthemum zawadskii* extract, 100 parts by weight of the *Catnip* extract, 100 parts by weight of the *Shepherd's Purse* extract, 100 parts by weight of the *Apple Mint* leaf extract, 100 parts by weight of the *Actinidia polygama* extract, and 100 parts by weight of the *Artemisia annua* extract, but is not limited thereto.

[0084] According to one embodiment of the present invention, the extracts of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, *Apple mint*, *Actinidia polygama*, and *Artemisia annua* may be included in a weight ratio of 1:1:1:1:1:1, but are not limited thereto.

[0085] In this invention, the term "pest" is used in the broadest sense, encompassing not only sanitary pests that transmit diseases to humans or livestock, such as mosquitoes, flies, and mites, or agricultural pests that damage crops, but also love bugs (scientific name: Plecia nearctica), mayflies, centipedes, millipedes, and other insects that cause visual or psychological discomfort or revulsion to humans simply by their existence.

[0086] According to an embodiment of the present invention, the pest may include any that can confirm repellent activity.

[0087] To specifically exemplify the effects of the present invention, the following experimental examples evaluated the repellent efficacy against mosquitoes, which are representative sanitary pests of the summer, and lovebugs, which have recently caused severe discomfort due to mass outbreaks; however, the scope of protection of the present invention is not limited to these pests only.

[0088] In the present invention, "Mosquito" refers to any insect belonging to the family Culicidae that can cause direct harm or transmit diseases by sucking blood from humans and animals, including species such as Aedes, Culex, and Anopheles, unless specifically limited to a specific species.

[0089] In the present invention, "Lovebug" refers to the red-backed velvet fly (scientific name: Plecia nearctica) belonging to the family Bibionidae of the order Diptera, and refers to an insect that does not directly transmit diseases to humans but causes inconvenience to the living environment by appearing in large numbers, causing visual aversion and mental discomfort, and corroding the paint surface of vehicles.

[0091] Another aspect of the present invention provides a method for manufacturing the insect repellent composition.

[0092] In this specification, specific details regarding the manufacturing method are substantially the same as those described in the composition section, unless otherwise stated. Effects of the invention

[0093] The insect repellent composition comprising a complex extract of natural raw materials according to the present invention and the method for manufacturing the same solve the problems of the prior art and have the following effects:

[0094] This invention is manufactured by completely excluding chemically synthesized ingredients, such as DEET, which are controversial regarding their harmfulness to the human body, and using only natural plant-derived raw materials known to be harmless to the human body. Therefore, it minimizes skin irritation and can be used with confidence by all users, ranging from infants and children to adults with sensitive skin.

[0095] Furthermore, the present invention maximizes the synergistic effect between each component by combining multiple natural extracts with different repellent mechanisms in optimal proportions. As a result, it exhibits significantly superior repellent activity compared to using a single natural component.

[0096] Furthermore, the present invention not only effectively blocks the approach of sanitary pests, such as mosquitoes that transmit infectious diseases like malaria and Japanese encephalitis, but also simultaneously exhibits an excellent repellent effect against nuisance pests, such as love bugs, which have recently emerged in large numbers, particularly in the metropolitan area, causing severe discomfort and property damage. This provides the convenience of being able to deal with various household pests with a single product.

[0097] In addition, the present invention provides a refreshing and subtle aroma unique to plants derived from complex extracts, rather than the artificial and unpleasant smell of conventional chemical repellents, so there is no aversion to the user. Furthermore, it has been developed into a formulation that is applied freshly to skin or clothing without stickiness, thereby significantly increasing consumer satisfaction.

[0098] The effects obtainable from the embodiments are not limited to those mentioned above, and other unmentioned effects can be clearly derived and understood by a person skilled in the art based on the detailed description below. Specific details for implementing the invention

[0099] The present invention will be described in more detail below by way of examples. However, these examples are intended to illustrate the invention and the scope of the invention is not limited by these examples. Furthermore, each component or feature may be considered optional unless otherwise explicitly stated. Each component or feature may be implemented in a form not combined with other components or features. Additionally, various embodiments may be constructed by combining some components and / or features. Some components or features of one embodiment may be included in another embodiment or replaced with corresponding components or features of another embodiment. Furthermore, terms not specifically defined in this specification should be understood to have the meaning commonly used in the technical field to which the present invention belongs.

[0101] Preparation Example 1

[0102] First, wash and prepare the Korean aster (whole plant), wild mint (above-ground parts), shepherd's purse (whole plant), and / or apple mint (leaves). Next, place the Korean aster, wild mint, shepherd's purse, and / or apple mint leaves in a steamer, and steam for 70 to 80 o Steam at a temperature of C for about 10 minutes.

[0103] Next, the steamed mountain aster, catnip, shepherd's purse, and / or apple mint leaves are 67-70 o After primary drying for 2 hours at a temperature of C, 57-60 o Dry it a second time for 7 hours at a temperature of C.

[0104] After cooling the above secondary dried *Sanggujeolcho*, *Gaebachalha*, *Naengi*, and / or *Apple Mint* leaves by leaving them at room temperature, place them in a rapid freezer at -50 to -40°C o Rapidly freeze at a temperature of C, then freeze completely for about 4 hours.

[0105] Then, the frozen Korean aster, catnip, shepherd's purse, and / or apple mint leaves were left at room temperature to thaw and aged for 6-7 hours.

[0106] The above-mentioned aged *Sanggujeolcho*, *Gaebachalha*, *Naengi* and / or apple mint leaves are crushed to facilitate extraction, 10 times the volume of water is added, and 50 to 60 o Reflux cooling extraction is performed at a temperature of C for about 5 hours.

[0107] Next, the above extract is filtered to separate the residue and the filtrate, and the filtrate is concentrated to produce *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, and / or apple mint leaves.

[0109] Preparation Example 2

[0110] First, wash and dry the Actinidia polygama (fruit) and / or Artemisia annua (whole plant), then grind them to facilitate extraction.

[0111] 5 times the volume of 70% (v / v) ethanol is added to the above-mentioned crushed Actinidia polygama and / or Artemisia annua, and reflux cooling extraction is performed for about 2 hours.

[0112] Then, the above extract is filtered to separate the residue, and concentrated using a vacuum concentrator to prepare Actinidia polygama and / or Artemisia annua extracts.

[0114] Examples

[0115] The above-prepared extracts of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, *Apple mint*, *Actinidia polygama*, and *Artemisia annua* were mixed in a weight ratio of 1:1:1:1:1:1 and used as the sample for the example.

[0117] Comparative Example 1

[0118] The above-prepared Actinidia polygama extract and Artemisia annua extract were mixed in a weight ratio of 1:1 and used as the sample for Comparative Example 1.

[0120] Comparative Example 2

[0121] In Preparation Example 1, a sample of Comparative Example 2 was prepared by a method substantially identical to that of the Example, except that the following method was used.

[0122] First, wash and crush the leaves of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, or apple mint.

[0123] Next, add 10 times the volume of water and 50 to 60 o Reflux cooling extraction is performed at a temperature of C for about 5 hours.

[0124] Next, the above extract was filtered to separate the residue and the filtrate, and the filtrate was concentrated to prepare the *Sanggujeolcho* extract, *Gaebachalpa* extract, *Naengi* extract, and *Apple Mint* leaf extract.

[0126] Experimental Example 1. Confirmation of Antioxidant Effect

[0127] The antioxidant effect test of the composition of the present invention was performed using a Cellular ROS Assay kit.

[0128] Specifically, skin fibroblasts are suspended in DMEM (added with 10% serum and 1% antibiotics) medium and incubated (37 o It was incubated for 24 hours in a 5% CO2 (C) incubator. Then, the sample was treated at a concentration of 2% (v / v) and incubated in an incubator (37 o After incubating for 1 hour in C, 5% CO2, 1 mM H2O2 and 20 M DCFDA (2'7'-dichloro-dihydro-fluorescein diacetate) were added, and the results were measured using a fluorescence spectrometer (excitation 485 nm, emission 535 nm).

[0129] [Calculation Formula]

[0130] Reactive oxygen species inhibition rate (%) = {(Untreated group - Treated group) / Untreated group} x 100

[0131] Sample Reactive oxygen species inhibition rate (%) Examples 46 Comparative Example 1 30 Comparative Example 2 38

[0132] Looking at the results in the table above, the composition of the example showed an excellent reactive oxygen species inhibition rate of 46% or more, and also showed an excellent reactive oxygen species inhibition rate compared to the composition of the comparative example.

[0134] Experimental Example 2. Confirmation of pest repellent effect

[0135] The intention was to confirm the repellent efficacy of the composition according to the present invention and to demonstrate the superiority of the composition and manufacturing method of the present invention by comparing and evaluating it with Comparative Example 1, which has a different combination of extract components, and Comparative Example 2, which has a different manufacturing method.

[0137] (1) Evaluation of mosquito repellent efficacy

[0138] Female adult Aedes aegypti mosquitoes aged 5 to 7 days, obtained from and reared by the National Institute of Vector-borne Diseases of the Korea Disease Control and Prevention Agency, were used as test insects. Blood-feeding activity was increased by removing sugar water for 12 hours prior to the experiment.

[0139] Fifty test insects were placed in an acrylic cage measuring 30 cm × 30 cm × 30 cm. After uniformly impregnating a cellulose sheet measuring 2 cm × 2 cm with an artificial sweat solution, 0.5 mL of the solvent from each sample of the above Example, Comparative Example 1, and Comparative Example 2, as well as the control group (ethanol), was spray-applied to each sheet. All sheets were air-dried at room temperature for 30 minutes and then fixed to the center of each individual cage.

[0140] The number of mosquitoes landing on the sheet surface for 1 minute was measured three times at different elapsed times (30 minutes, 1 hour, 2 hours, 4 hours) after application, and the average value was calculated.

[0142] (2) Evaluation of Lovebug Repellent Efficacy

[0143] Adult lovebugs (Plecia nearctica) that occurred in large numbers in 2025 were collected and tested in a laboratory environment for more than 24 hours (25±2 o After acclimating to C (relative humidity 60±5%), individuals with good activity were selected and used as test insects.

[0144] Fifty test insects were placed in an acrylic cage of the same specifications as the mosquito experiment. 0.5 mL of the solvent from each sample of the above Example, Comparative Example 1, Comparative Example 2, and the control group was spray-applied to a 2 cm × 2 cm pure cellulose sheet that had not been treated with artificial sweat.

[0145] All sheets were air-dried at room temperature for 30 minutes and then fixed to the center of each individual cage. The number of lovebugs landing on the sheet surface for 1 minute was measured three times at elapsed times after application (30 minutes, 1 hour, 2 hours, 4 hours) and the average value was calculated.

[0147] (3) Evaluation Indicator: Repellency Rate (%)

[0148] Based on the above experimental results, the avoidance rate for each test group by time period was calculated according to the following mathematical formula 2.

[0150] [Mathematical Formula 2]

[0151] Avoidance Rate (%) = {(CT) / C} x 100

[0152] (C is the average number of pests that landed on the control group sheet, T is the average number of pests that landed on the test group sheet)

[0153] division type 30 minutes 1 hour 2 hours 4 hours Examples mosquito 99.5 97.2 93.8 88.4 Lovebug 99.8 98.5 96.1 92.5 Comparative Example 1 mosquito 92.1 81.5 65.7 48.2 Lovebug 85.4 70.1 51.9 33.6 Comparative Example 2 mosquito 65.3 44.8 25.1 10.5 Lovebug 61.8 40.2 21.5 8.7 control group mosquito 0 0 0 0 Lovebug 0 0 0 0

[0155] As shown in the results of Table 2 above, the embodiment of the present invention exhibited significantly superior repellent efficacy and persistence against all pests and at all times compared to Comparative Example 1 and Comparative Example 2.

[0156] Specifically, Comparative Example 1 showed a high repellent rate initially but a distinct decrease in efficacy over time, while Comparative Example 2 showed low repellent efficacy from the beginning. On the other hand, the Example (combination of 6 types) stably maintained a very high repellent rate of about 90% even after 4 hours. This demonstrates the synergistic effect of the composition of the present invention.

[0158] Formulation example

[0159] 100 parts by weight of an acrylic adhesive solution are added to a mixing container equipped with a stirrer, and stirred slowly at a speed of 150 rpm.

[0160] Next, 20 parts by weight of the natural raw material complex extract prepared in the embodiment of the present invention are added slowly in small portions.

[0161] After all the extracts are added, the mixture is stirred for an additional 30 minutes to prepare an adhesive solution in which the active ingredients are uniformly dispersed within the adhesive solution.

[0162] Next, the solution prepared above is applied (coated) to the coating surface of the release layer (silicone-coated PET film) with a uniform thickness of 100 μm using a doctor blade.

[0163] 80 coated release layer oThe solvent in the adhesive solution is completely evaporated and removed by passing it through a hot air dryer of C for 10 minutes, and an adhesive layer containing an active ingredient is formed.

[0164] After the drying process is complete, a support layer (PE film) is uniformly laminated onto the adhesive layer of the release layer using a compression roller to prevent the formation of bubbles. The laminated fabric is then die-cut into a circular shape with a diameter of 3 cm using a press machine to complete individual patches.

[0166] The various embodiments described above may be embodied in other specific forms without departing from the technical idea and essential features. Accordingly, the above detailed description should not be interpreted restrictively in all respects but should be considered exemplary. The scope of the various embodiments shall be determined by a reasonable interpretation of the appended claims, and all modifications within the equivalent scope of the various embodiments are included within the scope of the various embodiments. Furthermore, embodiments may be constructed by combining claims that do not have an explicit citation relationship in the patent claims, or they may be included as new claims through amendments made after filing.

Claims

Claim 1 An insect repellent composition comprising extracts of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, apple mint leaf extract, *Actinidia polygama* extract, and *Artemisia annua*, wherein the *Chrysanthemum zawadskii* extract, *Mentha arvensis* extract, *Shepherd's purse* extract, or apple mint leaf extract is prepared by a method comprising the following steps: S1) 70 to 80 of *Chrysanthemum zawadskii*, *Mentha arvensis*, *Shepherd's purse*, or apple mint leaf. o Step of steaming at a temperature of C; S2) the steamed *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves at 60 to 80 o Step of primary drying at a temperature of C for 1 to 3 hours; S3) 50 to 60 of the primary dried leaves of *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint. o Step of secondary drying at a temperature of C for 5 to 10 hours; S4) drying the dried *Chrysanthemum zawadskii*, *Catnip*, *Shepherd's purse*, or apple mint leaves at -50 to -40 o A step of freezing at a temperature of C; S5) a step of aging the frozen Korean aster, catnip, shepherd's purse, or apple mint leaves at room temperature; S6) a step of crushing the aged Korean aster, catnip, shepherd's purse, or apple mint leaves; S7) adding the crushed Korean aster, catnip, shepherd's purse, or apple mint leaves to a solvent, and 50 to 60 o Step of extracting by reflux cooling at a temperature of C; and S8) step of filtering and concentrating the extract. Claim 2 The insect repellent composition according to claim 1, wherein the composition comprises, based on 100 parts by weight of the *Chrysanthemum zawadskii* extract, 80 to 120 parts by weight of *Catnip* extract, 80 to 120 parts by weight of *Shepherd's Purse* extract, 80 to 120 parts by weight of *Apple Mint* leaf extract, 80 to 120 parts by weight of *Actinidia polygama* extract, and 80 to 120 parts by weight of *Artemisia annua* extract. Claim 3 delete

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