Composition for anti-inflammation and skin regeneration comprising mixture of hemp extract and jellyfish hydrolysate as effective component

Abstract

The present invention relates to an anti-inflammatory and skin regeneration composition containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient. Since the mixture of the present invention has a significant effect of reducing the production of NO increased by LPS treatment and improving the skin cell regeneration ability reduced by UV irradiation compared to hemp extract or jellyfish hydrolysate alone, it can be usefully used as an anti-inflammatory and skin regeneration composition.

Description

Technology Field

[0001] The present invention relates to an anti-inflammatory and skin regeneration composition containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient. Background Technology

[0002] The inflammatory response is a defensive reaction of biological tissues against external stimuli, involved in the physiological and pathological processes of various diseases, and serves as a mechanism to repair or regenerate tissues damaged by external stimuli. A characteristic of this inflammatory response is that it entails physicochemical, chemical, and bacteriological changes in tissues such as skin and joints. Symptoms caused by inflammation include fever, redness, and edema; it is known that persistent inflammatory responses can lead to various intractable diseases, including dementia, cardiovascular disease, cancer, obesity, arthritis, and diabetes. When an inflammatory response occurs, inflammatory cytokines such as TNF-α, IL-1β, and IL-6 are secreted, and the secretion of these cytokines is promoted by NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells). Anti-inflammatory agents primarily used to treat inflammation are broadly classified into steroidal and non-steroidal anti-inflammatory drugs; however, since most synthetic anti-inflammatory agents often entail various side effects in addition to their primary effects, there is a need to develop anti-inflammatory agents that are highly effective with minimal side effects.

[0003] Meanwhile, the skin serves as the body's primary defense barrier, protecting internal organs from external environmental stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants, while playing a crucial role in maintaining biological homeostasis, including thermoregulation. The stratum corneum, the outermost layer of the skin, is a tissue formed from the transformation of skin cells, and the stratum corneum is composed entirely of dead cells. As the loosely formed cell layer within the connective tissue of the dermis ascends to the epidermis, the cells lose their vitality and transform into a solid and regular layer. The nuclei and cytoplasm escape, forming a stratum corneum that is as hard as bricks. This stratum corneum strengthens the skin while acting as a defensive line that retains the body's moisture at the outermost layer of the skin tissue and protects against various substances entering from the outside.

[0004] The processes of skin cell generation and keratinization are crucial cycles for maintaining skin health. When skin cells have good regenerative capacity, they are rapidly generated in the dermis and keratinize quickly. This allows damaged and aged keratin caused by external stimuli to be promptly replaced by newly generated keratin, helping to maintain healthy skin. Just like other organs in the body, the skin's regenerative capacity declines with age, leading to gradual aging. Consequently, skin elasticity is lost, old keratin fails to be replaced and becomes keratinized, and the structures and fibers that maintain elasticity diminish, resulting in the formation of wrinkles and skin atrophy.

[0005] Therefore, there have been continuous efforts to develop bioactive substances with skin regeneration-promoting effects in order to enhance this skin regeneration ability, maintain healthy skin, and preserve healthy, elastic skin. Tretinoin (trans-retinoic acid) and retinol began to be used as cosmetic ingredients, and since then, substances similar to female hormones and antioxidants extracted from various plants have been introduced into cosmetics as ingredients with such efficacy. However, most of these cosmetic ingredients had various problems, such as insufficient efficacy or causing side effects like skin irritation. Accordingly, research is actively being conducted on ingredients that promote skin regeneration without causing skin side effects.

[0006] Meanwhile, Korean Registered Patent No. 1915952 discloses a "composition for antioxidant, anti-inflammatory, and skin regeneration containing Houttuynia cordata extract as an active ingredient," and Korean Published Patent No. 2022-0141513 discloses a "composite processed product of ginseng above-ground parts and soybean protein with enhanced antioxidant, whitening, skin regeneration, and / or anti-inflammatory activity, a composition containing the same, and a method for manufacturing the same," but there is no disclosure at all regarding the "composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient" of the present invention. The problem to be solved

[0007] The present invention was derived from the above requirements. The present invention provides an anti-inflammatory and skin regeneration composition containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient, and the present invention was completed by confirming that the mixture of hemp extract and jellyfish hydrolysate according to the present invention significantly increases the anti-inflammatory and skin regeneration ability reduced by UV irradiation compared to the hemp extract or jellyfish hydrolysate at a concentration that is non-cytotoxic. means of solving the problem

[0008] To solve the above problem, the present invention provides a cosmetic composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0009] In addition, the present invention provides a health functional food composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0010] In addition, the present invention provides a pharmaceutical composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0011] In addition, the present invention provides a quasi-drug composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0012] In addition, the present invention provides a feed additive for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0013] In addition, the present invention provides a veterinary composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient. Effects of the invention

[0014] The present invention relates to an anti-inflammatory and skin regeneration composition containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient. Since the mixture of the present invention has a significant effect of reducing the production of NO increased by LPS treatment and improving the skin cell regeneration ability reduced by UV irradiation compared to hemp extract or jellyfish hydrolysate alone, it can be usefully used as an anti-inflammatory and skin regeneration composition. Brief explanation of the drawing

[0015] Figure 1 shows the results of confirming the cytotoxicity of a mixture of hemp extract and jellyfish hydrolysate of the present invention. CON is a negative control group with no treatment, JH is a group treated with jellyfish hydrolysate, HE is a group treated with hemp seed extract, and JH+HE is a group treated with a mixture of jellyfish hydrolysate and hemp seed extract. Figure 2 shows the results of confirming the inhibitory effect of the mixture of hemp extract and jellyfish hydrolysate of the present invention on NO (nitric oxide) production in mouse macrophages, RAW 264.7 cells. CON is a negative control group with no treatment, LPS is a control group treated with LPS alone, an NO production inducing substance, RE is a positive control group treated with resveratrol, JH is a jellyfish hydrolysate treatment group, HE is a hemp seed extract treatment group, and JH+HE is a group treated with a mixture of jellyfish hydrolysate and hemp seed extract. @@@ indicates that the amount of NO produced in the LPS-alone treatment group increased statistically significantly compared to CON, and p<0.001. ## and ### indicate that the amount of NO produced in the sample treatment group decreased statistically significantly compared to the LPS-alone treatment group, and ## is p<0.01 and ### is p<0.001. **, *** indicate that the amount of NO produced in the group treated with the mixture of jellyfish hydrolysate and hemp seed extract was statistically significantly reduced compared to the group treated with jellyfish hydrolysate alone, with ** being p<0.01 and *** being p<0.001. $$, $$$ indicate that the amount of NO produced in the group treated with the mixture of jellyfish hydrolysate and hemp seed extract was statistically significantly reduced compared to the group treated with hemp seed extract alone, with $$ being p<0.01 and $$$ being p<0.001. Figure 3 shows the results of confirming the cell regenerative ability of the mixture of hemp extract and jellyfish hydrolysate of the present invention in human fibroblast Hs68 cells. CON is a negative control with no treatment, UV is a control in which cell regenerative ability is reduced by ultraviolet irradiation, JH is the jellyfish hydrolysate treatment group, HE is the hemp seed extract treatment group, and JH+HE is the mixture of jellyfish hydrolysate and hemp seed extract treatment group. @@@ indicates that the cell regenerative ability of the UV-alone treatment group was statistically significantly reduced compared to CON, and p<0.001. # and ### indicate that the cell regenerative ability of the sample treatment group was statistically significantly increased compared to the UV-alone treatment group, and # is p<0.05 and ### is p<0.001. * indicates that the cell regeneration ability of the group treated with a mixture of jellyfish hydrolysate and hemp seed extract increased statistically significantly compared to the group treated with jellyfish hydrolysate alone, and * is p<0.05. $$$ indicates that the cell regeneration ability of the group treated with a mixture of jellyfish hydrolysate and hemp seed extract increased statistically significantly compared to the group treated with hemp seed extract alone, and $$$ is p<0.001. Specific details for implementing the invention

[0016] To achieve the objective of the present invention, the present invention provides a cosmetic composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0017] The extraction solvent of the hemp extract above may be water, C1 to C4 lower alcohols or a mixture thereof, preferably ethanol, but is not limited thereto.

[0018] The above hemp extract can be extracted using any part of the hemp, and preferably is an extract of hemp seeds, but is not limited thereto.

[0019] The above hemp is preferably of the blue hemp variety, but is not limited thereto.

[0020] The above jellyfish hydrolysate may be hydrolyzed using a protease, preferably using an endo-protease, and more preferably using an alcalase, but is not limited thereto.

[0021] In one embodiment of the present invention, the mixture may be a mixture of 0.5 to 2 parts by weight of jellyfish hydrolysate with 1 part by weight of hemp extract, preferably a mixture of 0.8 to 1.2 parts by weight of jellyfish hydrolysate with 1 part by weight of hemp extract, and more preferably a mixture of 1 part by weight of jellyfish hydrolysate with 1 part by weight of hemp extract, but is not limited thereto.

[0022] In one embodiment of the present invention, the composition has the effect of preventing or improving skin cell damage caused by ultraviolet rays.

[0023] The cosmetic composition for anti-inflammatory and skin regeneration according to the present invention may have any one formulation selected from the group consisting of cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, nourishing essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser, but is not limited thereto. The cosmetic composition made of each of these formulations may contain various bases and additives that are necessary and appropriate for the formulation of the formulation, and the types and amounts of these ingredients can be easily selected by a person skilled in the art.

[0024] In the case where the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0025] When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane butane, or dimethyl ether.

[0026] In the case where the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvating agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0027] In the case where the formulation of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used as carrier components.

[0028] In the case where the formulation of the cosmetic composition of the present invention is a cleansing product containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0029] In addition, the present invention provides a health functional food composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0030] When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as is, used together with other foods or food ingredients, or used appropriately according to conventional methods. The active ingredient may be used appropriately according to its intended use (prevention or improvement). Generally, when manufacturing food or beverages, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, relative to the raw material. However, in the case of long-term consumption for health purposes, the above amount may be less than the above range, and since there are no issues regarding safety, the active ingredient may be used in an amount greater than the above range.

[0031] There are no special restrictions on the types of the above-mentioned health functional foods. Examples of foods to which the above-mentioned health functional food composition may be added include meat, sausage, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea drinks, alcoholic beverages, and vitamin complexes, and include all health foods in the conventional sense.

[0032] In addition, the health functional food composition of the present invention may be manufactured into a food, particularly a functional food. The functional food of the present invention may include ingredients that are typically added. For example, it includes proteins, carbohydrates, fats, nutrients, and flavoring agents. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredients. The natural carbohydrate is preferably a monosaccharide (e.g., glucose, fructose, etc.), a disaccharide (e.g., maltose, sucrose, etc.), an oligosaccharide, a polysaccharide (e.g., dextrin, cyclodextrin, etc.), or a sugar alcohol (e.g., xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., taumatin, stevia extract, etc.) or a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

[0033] In addition to the above health functional food composition, it may further contain various nutritional agents, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. Although the proportion of these added ingredients is not of great importance, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health functional food composition of the present invention.

[0034] In addition, the present invention provides a pharmaceutical composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0035] In one embodiment of the present invention, the composition can treat skin wounds caused by ultraviolet rays, but is not limited thereto.

[0036] The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier in addition to the active ingredient, and such carrier is one that is commonly used in formulations and includes, but is not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition to the above ingredients, the pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, etc.

[0037] Suitable dosages of the pharmaceutical composition according to the present invention can be prescribed in various ways depending on factors such as the formulation method, mode of administration, patient's age, body weight, sex, pathological condition, food, time of administration, route of administration, excretion rate, and response sensitivity.

[0038] The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by topical application to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc.

[0039] The concentration of the active ingredient included in the composition of the present invention can be determined by considering the purpose of treatment, the patient's condition, the required duration, etc., and is not limited to a specific range of concentrations.

[0040] In addition, the present invention provides a quasi-drug composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0041] In one embodiment of the present invention, the composition can treat skin wounds caused by ultraviolet rays, but is not limited thereto.

[0042] The term "quasi-drug" as used in the present invention refers to articles used for the purpose of diagnosing, treating, improving, alleviating, managing, or preventing diseases in humans or animals, among which the effect is milder than that of pharmaceuticals. For example, according to the Pharmaceutical Affairs Act, quasi-drugs are defined as articles excluding those used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans or animals, and products that have a mild effect on the human body or do not act directly on it. The quasi-drug composition of the present invention is used for the purpose of treating skin wounds and is not particularly limited in its formulation; for example, it may be a transdermal formulation such as a lotion, ointment, gel, cream, patch, or spray.

[0043] In addition, for each formulation, the quasi-drug composition may arbitrarily select and combine other ingredients according to the formulation or purpose of use of other quasi-drugs. The amount of the active ingredient can be appropriately determined according to the purpose of use (inhibition or alleviation). For example, it may include conventional adjuvants such as thickeners, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers.

[0044] In addition, the present invention provides a feed additive for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0045] The feed additive of the present invention corresponds to an auxiliary feed under the Feed Management Act. In the present invention, the term "feed" may refer to any natural or artificial prescribed food, single meal, etc., or the components of said single meal, intended for or suitable for animals to eat, consume, and digest. The types of said feed are not particularly limited, and feeds commonly used in the relevant technical field may be used. Non-limiting examples of said feed include plant-based feeds such as grains, root vegetables, food processing by-products, algae, fibers, pharmaceutical by-products, oils and fats, starches, meal, or grain by-products; and animal-based feeds such as proteins, inorganic substances, oils and fats, minerals, single-cell proteins, zooplankton, or food waste. These may be used individually or in a mixture of two or more types.

[0046] In addition, the present invention provides a veterinary composition for anti-inflammatory and skin regeneration containing a mixture of hemp extract and jellyfish hydrolysate as an active ingredient.

[0047] The veterinary composition of the present invention may further include suitable excipients and diluents according to conventional methods. Examples of excipients and diluents that may be included in the veterinary composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, cetanol, stearyl alcohol, liquid paraffin, sorbitan monostearate, polysorbate 60, methylparaben, propylparaben, and mineral oil.

[0048] The veterinary composition according to the present invention may further include fillers, anticoagulants, lubricants, wetting agents, spices, emulsifiers, preservatives, etc., and the veterinary composition according to the present invention may be formulated using methods well known in the art to provide rapid, sustained, or delayed release of the active ingredient after administration to an animal, and the formulation may be in the form of powders, granules, tablets, capsules, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, suppositories, sterile injectable solutions, sterile topical preparations, etc.

[0049] The effective amount of the veterinary composition according to the present invention can be appropriately selected according to the individual animal. It may be determined based on the severity of the disease or condition, the sensitivity to the active ingredient of the present invention according to the individual's age, weight, health status or gender, the route of administration, the duration of administration, factors including other compositions combined with or used simultaneously with the composition, and other factors well known in the physiological or veterinary field.

[0051] The present invention will be described in more detail below using manufacturing examples and embodiments. These manufacturing examples and embodiments are intended solely to explain the present invention more specifically, and it is obvious to those skilled in the art that the scope of the present invention is not limited by them.

[0053] Preparation Example 1. Preparation of Hemp Seed Extract (HE)

[0054] After grinding hot-air dried hemp seeds at 55℃, 10 parts by weight of 70% (v / v) ethanol were added to 1 part by weight of hemp seed powder, and then extracted by stirring (180 rpm) at room temperature for 3 hours. After separating the supernatant and precipitate by centrifugation at 3000 rpm, the supernatant was concentrated using a vacuum concentrator and then freeze-dried to obtain a hemp seed extract (HE) sample.

[0056] Preparation Example 2. Preparation of jellyfish hydrolysate (JH)

[0057] 5 parts by weight of purified water were added to 1 part by weight of raw jellyfish, and the stirring process was repeated twice to wash and desalt the jellyfish, after which it was dried with hot air at 55°C. After that, the dried jellyfish was ground to 40 mesh, and then the jellyfish powder was treated with an alkalase from Novozymes to proceed with hydrolysis.

[0058] Specifically, hydrolysis was carried out by adding 5 parts by weight of purified water to 1 part by weight of jellyfish powder, and then proceeding for 6 hours under conditions of pH 8 and 55℃.

[0059] After hydrolysis, the precipitate and supernatant were separated by centrifugation (3000 rpm), the supernatant was concentrated using a vacuum concentrator, and then dried by spray drying (60°C) to obtain a jellyfish hydrolysate (JH) sample.

[0061] Preparation Example 3. Preparation of a mixture of hemp seed extract and jellyfish hydrolysate

[0062] An equal amount of the jellyfish hydrolysate sample of Preparation Example 2 was mixed with 1 part by weight of the hemp seed extract sample of Preparation Example 1 and used as a sample of a mixture of hemp seed extract and jellyfish hydrolysate (JH+HE).

[0064] Example 1. Confirmation of anti-inflammatory effect

[0065] 1) Cytotoxicity Assessment

[0066] To determine whether the samples of Preparation Examples 1 to 3 of the present invention exhibit cytotoxicity, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazilium bromide) analysis was performed using the method of Mosmann (1983).

[0067] RAW 264.7 mouse macrophages were purchased from ATCC and cultured in DMEM medium containing 10% (v / v) FBS and 1% (v / v) penicillin at 37°C and 5% CO2.

[0068] 1×10 using DMEM medium containing 10%(v / v) FBS and 1%(v / v) penicillin 6 RAW 264.7 cell dilution diluted to cell / mL was dispensed at a rate of 200 µl per well of a 96-well plate and cultured for 24 hours. Afterward, the medium was replaced with DMEM medium that did not contain FBS or penicillin, and each sample of Preparation Examples 1 to 3, diluted in the same DMEM medium, was treated at a concentration of 50–200 µg / mL. After one day, 20 µl of MTT reagent was dispensed per well and reacted for 1 hour and 30 minutes. Then, the medium was removed, and the formed formazan was dissolved in DMSO to measure the absorbance.

[0069] As a result, as disclosed in FIG. 1, it was confirmed that the samples of Preparation Examples 1 to 3 of the present invention did not exhibit cytotoxicity up to a concentration of 200 μg / mL.

[0071] 2) Measurement of NO (Nitric Oxide) Production Amount

[0072] To measure NO production, 1×10⁶ RAW 264.7 cells were placed in a 96-well plate. 5 After dispensing at a rate of cell / mL and incubating for 24 hours, the medium was replaced with DMEDM medium that does not contain FBS, and the samples of Preparation Examples 1 to 3 were treated. After 1 hour, LPS (1 μg / mL) was added and incubated for 24 hours. After incubation, 100 μl of the culture medium was transferred to a new 96-well plate, 100 μl of grease reagent was added, and the reaction was allowed to proceed for 10 minutes. The absorbance was then measured at 540 nm, and the amount of NO produced was calculated as a percentage relative to the group treated with LPS alone.

[0073] As a result, as disclosed in FIG. 2, the jellyfish hydrolysate did not show a reduction in NO production when used alone, and the hemp seed extract showed a reduction in NO production at a concentration of 100 μg / mL or higher, but the mixture of jellyfish hydrolysate and hemp seed extract showed a reduction in NO production even at low concentrations, and at the same concentration, the mixture of the present invention showed a significant anti-inflammatory effect compared to jellyfish hydrolysate alone or hemp seed extract alone.

[0075] Example 2. Confirmation of skin cell regenerative ability

[0076] The skin cell regenerative ability of a mixture of jellyfish hydrolysate and hemp seed extract was confirmed in human skin fibroblasts, Hs68 cells.

[0077] Hs68 cells were purchased from ATCC and cultured in DMEM medium containing 10% (v / v) FBS and 1% (v / v) penicillin at 37°C and 5% CO2.

[0078] 2×10⁶ Hs68 cells in a 24-well plate 5 Cells were dispensed at a density of cells / mL and cultured in a 37°C, 5% CO2 incubator for 24 hours. Afterward, the medium was replaced with 50 µl of PBS and irradiated with UV light for 40 seconds. Subsequently, the medium was replaced with DMEM, and the samples of Preparation Examples 1 to 3 were treated at a concentration of 200 µg / mL and cultured for 48 hours. Afterward, the MTT solution was treated for 3 hours, the medium was removed, and the resulting formazan was dissolved in 100 µl of DMSO. The absorbance was then measured at 540 nm to confirm the skin cell regenerative capacity.

[0079] As a result, as disclosed in FIG. 3, it was confirmed that the skin cell regeneration ability reduced by UV irradiation increased when the samples of Preparation Examples 1 to 3 of the present invention were treated, and in particular, the skin cell regeneration ability was significantly increased when the samples were mixed compared to jellyfish hydrolysate alone and hemp seed extract alone.

Claims

Claim 1 A cosmetic composition for preventing or improving skin cell damage caused by ultraviolet rays and for anti-inflammatory purposes, containing as an active ingredient a mixture of hemp extract and jellyfish hydrolysate, wherein 0.5 to 2 parts by weight of jellyfish hydrolysate hydrolyzed using alcalase are mixed with 1 part by weight of hemp extract. Claim 2 A cosmetic composition for preventing or improving skin cell damage caused by inflammation and ultraviolet rays, characterized in that, in claim 1, the extraction solvent of the hemp extract is water, a C1-C4 lower alcohol, or a mixture thereof. Claim 3 A cosmetic composition for preventing or improving skin cell damage caused by inflammation and ultraviolet rays, characterized in that, in claim 1, the hemp extract is an extract of hemp seeds. Claim 4 delete Claim 5 delete Claim 6 delete Claim 7 A health functional food composition for preventing or improving skin cell damage caused by ultraviolet rays and for anti-inflammatory purposes, containing as an active ingredient a mixture of hemp extract and jellyfish hydrolysate, wherein 0.5 to 2 parts by weight of jellyfish hydrolysate hydrolyzed using alcalase are mixed with 1 part by weight of hemp extract. Claim 8 An anti-inflammatory pharmaceutical composition containing, as an active ingredient, a mixture of hemp extract and jellyfish hydrolysate, wherein 0.5 to 2 parts by weight of jellyfish hydrolysate hydrolyzed using alcalase is mixed with 1 part by weight of hemp extract. Claim 9 An anti-inflammatory quasi-drug composition containing, as an active ingredient, a mixture of hemp extract and jellyfish hydrolysate, wherein 0.5 to 2 parts by weight of jellyfish hydrolysate hydrolyzed using alcalase is mixed with 1 part by weight of hemp extract. Claim 10 A feed additive for preventing or improving skin cell damage caused by ultraviolet rays and for anti-inflammatory purposes, containing as an active ingredient a mixture of hemp extract and jellyfish hydrolysate, wherein 0.5 to 2 parts by weight of jellyfish hydrolysate hydrolyzed using alcalase are mixed with 1 part by weight of hemp extract. Claim 11 An anti-inflammatory veterinary composition containing, as an active ingredient, a mixture of hemp extract and jellyfish hydrolysate, wherein 0.5 to 2 parts by weight of jellyfish hydrolysate hydrolyzed using alcalase is mixed with 1 part by weight of hemp extract.

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