Pet gum

Abstract

The present invention provides a pet chew comprising low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, and hexametaphosphate (SHMP).

Description

Technology Field

[0001] The present invention relates to chewing gum for pets. Background Technology

[0002] Just as with humans, dental health is a very important factor for pets. Preventing tartar buildup or removing accumulated tartar is crucial for their dental health. In pets, plaque turns the entire tooth yellow, and as tartar accumulates near the gums, it is unsightly, a cause of bad breath, and eventually leads to tooth loss or gum disease.

[0003] Since pets cannot brush their own teeth and oral care like humans, people can often brush them on their behalf, but this is not a desirable method considering the pet itself and the disposal of the mouthwash after brushing.

[0004] On the other hand, scaling can be performed as a veterinary treatment at animal hospitals, but this is not a desirable method in terms of the pet's health and economic costs.

[0005] In addition, by providing various functionalities to the chewing gum to improve its function as a snack, as well as to the dental health of pets, it can contribute to the health and well-being of pets. Prior art literature

[0006] Patent Document 1: Korean Published Patent No. 10-2021-0096807 The problem to be solved

[0007] The objective of the present invention is to provide a pet chewing gum manufactured by adding various functional ingredients.

[0008] The problems of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0009] To achieve the above objective, the present invention

[0010] A pet chew is provided comprising low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, and hexametaphosphate (SHMP).

[0011] In addition, the pet gum contains low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, hexametaphosphate (SHMP), rice flour, cellulose, glycerin, propylene glycol, sweet potato starch, hyaluronic acid, flaxseed oil, salmon oil, fucoidan, astaxanthin, Bacillus coagulans, fructooligosaccharide, yucca root extract, sorbitol, vitamin A, vitamin B12, vitamin D, vitamin E, zinc, manganese, DL-methionine, L-lysine, and potassium sorbate.

[0012] In addition, the pet gum comprises 1-5 parts by weight of low molecular weight fish collagen with a molecular weight of 10-1,000 Da, 4-8 parts by weight of shrimp hydrolysate with a molecular weight of 1-500 Da, 0.001-0.003 parts by weight of propolis, 0.22-0.26 parts by weight of spirulina, 0.832-0.836 parts by weight of hexametaphosphate (SHMP), 56-60 parts by weight of rice flour, 6-10 parts by weight of cellulose, 6-10 parts by weight of glycerin, 6-10 parts by weight of propylene glycol, 4-8 parts by weight of sweet potato starch, 0.001-0.003 parts by weight of hyaluronic acid, 0.001-0.003 parts by weight of flaxseed oil, 0.001-0.003 parts by weight of salmon oil, and 0.001-0.003 parts by weight of fucoidan. parts by weight, astaxanthin 0.001-0.003 parts by weight, Bacillus coagulans 0.001-0.003 parts by weight, fructooligosaccharide 0.001-0.003 parts by weight, yucca root extract 0.001-0.003 parts by weight, sorbitol 1.7-2.1 parts by weight, vitamin A 0.0003-0.0007 parts by weight, vitamin B12 0.0003-0.0007 parts by weight, vitamin D 0.0003-0.0007 parts by weight, vitamin E 0.0003-0.0007 parts by weight, zinc 0.0008-0.0012 parts by weight, manganese 0.0008-0.0012 parts by weight, DL-methionine 0.0008-0.0012 It contains parts by weight, 0.0008-0.0012 parts by weight of L-lysine and 0.001-0.003 parts by weight of potassium sorbate.

[0013] In addition, the above pet chew further comprises 1-5 parts by weight of fermented functional powder, and

[0014] The above-mentioned fermented functional powder is characterized as an extract prepared by fermenting a complex of graviola leaves, prickly pear stems, and true needle mushrooms mixed in a weight ratio of 1:1:1 and then extracting it.

[0015] In addition, the above pet chew further comprises 1-5 parts by weight of fermented functional powder, and

[0016] The above-mentioned fermented functional powder is,

[0017] The method is characterized by being prepared by performing the following steps: adding 48-52 parts by weight of a complex mixture of graviola leaves, prickly pear stems, and *Cham-ba-neul-mushroom* in a weight ratio of 1:1:1 and 48-52 parts by weight of purified water, and adding 0.8-1.2 parts by weight of a mixed strain of Aspergillus oryzae and Sporobolomyces papyii in a weight ratio of 1:1, followed by fermentation at a temperature of 33-37℃ for 2-4 days; after fermentation, adding 38-42 parts by weight of hexane and extracting at a temperature of 88-92℃ for 11-13 hours to prepare an extract; and concentrating the extract under reduced pressure and freeze-drying it.

[0018] In addition, the present invention

[0019] A step of preparing a fermented functional powder comprising: adding 48-52 parts by weight of a complex of graviola leaves, prickly pear stems, and *Cham-ba-neul-mushroom* mixed in a weight ratio of 1:1:1 and 48-52 parts by weight of purified water, and adding 0.8-1.2 parts by weight of a mixed strain of Aspergillus oryzae and Sporobolomyces papyii mixed in a weight ratio of 1:1, followed by fermentation at a temperature of 33-37℃ for 2-4 days; a step of preparing an extract by adding 38-42 parts by weight of hexane after fermentation and extracting at a temperature of 88-92℃ for 11-13 hours; and a step of concentrating the extract under reduced pressure and freeze-drying it.

[0020] 1-5 parts by weight of the above-mentioned fermented functional powder, 1-5 parts by weight of low molecular weight fish collagen with a molecular weight of 10-1,000 Da, 4-8 parts by weight of shrimp hydrolysate with a molecular weight of 1-500 Da, 0.001-0.003 parts by weight of propolis, 0.22-0.26 parts by weight of spirulina, 0.832-0.836 parts by weight of hexametaphosphate (SHMP), 56-60 parts by weight of rice flour, 6-10 parts by weight of cellulose, 6-10 parts by weight of glycerin, 6-10 parts by weight of propylene glycol, 4-8 parts by weight of sweet potato starch, 0.001-0.003 parts by weight of hyaluronic acid, 0.001-0.003 parts by weight of flaxseed oil, 0.001-0.003 parts by weight of salmon oil, and fucoidan. 0.001-0.003 parts by weight, astaxanthin 0.001-0.003 parts by weight, Bacillus coagulans 0.001-0.003 parts by weight, fructooligosaccharide 0.001-0.003 parts by weight, yucca root extract 0.001-0.003 parts by weight, sorbitol 1.7-2.1 parts by weight, vitamin A 0.0003-0.0007 parts by weight, vitamin B12 0.0003-0.0007 parts by weight, vitamin D 0.0003-0.0007 parts by weight, vitamin E 0.0003-0.0007 parts by weight, zinc 0.0008-0.0012 parts by weight, manganese 0.0008-0.0012 parts by weight, DL-methionine A step of mixing 0.0008-0.0012 parts by weight of L-lysine, 0.0008-0.0012 parts by weight of L-lysine, and 0.001-0.003 parts by weight of potassium sorbate and feeding the mixture into an extruder; and

[0021] A method for manufacturing a pet chewing gum is provided, comprising the steps of extruding with an extruder, cooling, and cutting. Effects of the invention

[0022] The pet gum according to the present invention is a gum comprising low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, and hexametaphosphate (SHMP). By applying low molecular weight fish collagen with a molecular weight of 1,000 Da or less and hydrolyzed protein with a molecular weight of 500 Da or less, nutrients are broken down into very small sizes to minimize immune responses, prevent dietary allergies to the substances applied as protein sources, and promote oral health by applying propolis, spirulina, and hexametaphosphate. Specific details for implementing the invention

[0023] Various embodiments are described in more detail below. The embodiments described herein may be modified in various ways. Specific embodiments may be described in detail in the detailed description. However, the specific embodiments disclosed are intended only to facilitate understanding of various embodiments. Accordingly, the technical concept is not limited by the specific embodiments disclosed, and it should be understood that it includes all equivalents or substitutions that fall within the spirit and scope of the invention.

[0024] Terms including ordinal numbers, such as first, second, first, second, etc., may be used to describe various components, but these components are not limited by the aforementioned terms. The aforementioned terms are used solely for the purpose of distinguishing one component from another.

[0025] In this specification, terms such as "comprising" or "having" are intended to specify the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof. When a component is described as being "connected" or "connected" to another component, it should be understood that it may be directly connected to or connected to that other component, or that there may be other components in between. On the other hand, when a component is described as being "directly connected" or "directly connected" to another component, it should be understood that there are no other components in between.

[0026] Furthermore, in describing the present invention, if it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the essence of the invention, such detailed description is abbreviated or omitted.

[0027] The present invention

[0028] A pet chew is provided comprising low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, and hexametaphosphate (SHMP).

[0029] Hereinafter, the pet chewing gum according to the present invention will be described in detail.

[0031] Fish collagen is collagen derived from fish, primarily extracted from dried pollack skin, eel, shark, saury, flounder, skate, salmon, etc. Collagen obtained from fish skin or fish scales is similar to the structure of the human body, resulting in an absorption rate of 84%. It is readily available on the market and has the advantage of relatively higher absorption by the human body due to its smaller molecular weight compared to animal-derived collagen. Nevertheless, because the molecular weight of collagen itself is large, the absorption rate—which allows it to be absorbed into the small intestine and reach the skin—is significantly low. The fish collagen presented in this invention is a low-molecular-weight fish collagen with a molecular weight of 10-1,000 Da, and its application results in excellent absorption. Low-molecular-weight fish collagen with a molecular weight of 1,000 Da or less is rapidly absorbed in the digestive system and has a significantly higher bioavailability compared to general collagen. Absorption rates up to 200 times higher than those of general collagen have been reported.

[0032] Low molecular weight fish collagen stimulates fibroblasts (cells responsible for skin regeneration) in skin and hair tissues to induce increased collagen production. As a result, the protective barrier of the skin and coat is strengthened, and it helps improve skin hydration and coat quality. It can also contribute to skin recovery and the maintenance of elasticity even after damage caused by external stimuli such as UV rays. Furthermore, low molecular weight fish collagen acts as a raw material for collagen, a major protein in joint and bone tissues. Because it is easily absorbed into the body, it is expected to be effective in promoting joint health, strengthening cartilage, and improving mobility in pets.

[0033] Shrimp hydrolysate is a natural functional ingredient produced by enzymatically hydrolyzing shrimp protein into a low-molecular-weight peptide form with a molecular weight of 500 Da or less. By utilizing this low-molecular-weight shrimp hydrolysate as a protein source, it helps prevent dietary allergies while excluding major allergy-causing ingredients such as beef, chicken, and wheat. Furthermore, it boasts excellent absorption rates in the bodies of pets and offers various health-promoting benefits. Containing essential amino acids and bioactive peptides beneficial for skin regeneration and coat health, shrimp-derived hydrolysate contributes to strengthening the skin barrier, enhancing moisture retention, and improving coat shine and health. It can also aid in the recovery of skin damage caused by external environmental factors, such as UV rays. Additionally, the active ingredients in shrimp hydrolysate inhibit bacterial growth in the oral cavity, reducing plaque formation and bad breath, and contributing to the overall improvement of oral hygiene.

[0034] For example, shrimp can be prepared by washing and removing foreign substances, then hydrolyzing them at 50-60°C using protein-degrading enzymes such as Flavourzyme and / or Alcalase, and then concentrating or drying after filtration and centrifugation to remove insoluble substances.

[0035] Propolis is a natural resinous substance produced by honeybees (*Apis mellifera*) by collecting tree buds, sap, and resin and mixing them with their saliva and beeswax. It serves to inhibit pathogens, fungi, and bacteria within the hive and protect it from external intrusions. Its main components include flavonoids, phenolic acids, artepillin C, and caffeic acid phenethyl ester (CAPE), and these bioactive substances exhibit powerful antibacterial, antioxidant, and anti-inflammatory effects. Propolis is highly regarded as a health supplement for dogs due to its excellent ability to improve oral health and immunity in animals, particularly companion animals, as well as humans. By incorporating propolis into pet chewing gum, it helps prevent various inflammatory diseases and bacterial infections in the mouth, such as gingivitis, periodontitis, and bad breath. Furthermore, its essential fatty acids and antioxidants aid in skin regeneration, maintain and improve the shine and health of the fur, and have a positive effect on dry skin or brittle coats.

[0036] Spirulina is a blue-green microalgae that serves as a natural nutritional source rich in vitamins, minerals, essential amino acids, chlorophyll, beta-carotene, and antioxidants. It is gaining attention for its excellent nutritional value and functional properties. When applied to pet chewing gums, it inhibits plaque formation in the oral cavity and improves gum health and hygiene by suppressing bacterial growth through the action of its antioxidants and natural micronutrients. Furthermore, the abundant antioxidants contained in spirulina, such as chlorophyll and beta-carotene, protect cells from free radicals and are effective in boosting systemic immunity. When applied to pet chewing gums, the nutritional components of spirulina come into direct contact with the oral mucosa and gums during chewing, providing comprehensive support not only for oral health but also for overall health, skin, and coat health.

[0037] Hexametaphosphate is a type of inorganic phosphate that acts on the surface of a pet's teeth to help improve oral health.

[0038] In addition, the pet gum preferably comprises low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, hexametaphosphate (SHMP), rice flour, cellulose, glycerin, propylene glycol, sweet potato starch, hyaluronic acid, flaxseed oil, salmon oil, fucoidan, astaxanthin, Bacillus coagulans, fructooligosaccharide, yucca root extract, sorbitol, vitamin A, vitamin B12, vitamin D, vitamin E, zinc, manganese, DL-methionine, L-lysine, and potassium sorbate, and comprises 1-5 parts by weight of low molecular weight fish collagen with a molecular weight of 10-1,000 Da, 4-8 parts by weight of shrimp hydrolysate with a molecular weight of 1-500 Da, and propolis. 0.001-0.003 parts by weight, spirulina 0.22-0.26 parts by weight, hexametaphosphate (SHMP) 0.832-0.836 parts by weight, rice flour 56-60 parts by weight, cellulose 6-10 parts by weight, glycerin 6-10 parts by weight, propylene glycol 6-10 parts by weight, sweet potato starch 4-8 parts by weight, hyaluronic acid 0.001-0.003 parts by weight, flaxseed oil 0.001-0.003 parts by weight, salmon oil 0.001-0.003 parts by weight, fucoidan 0.001-0.003 parts by weight, astaxanthin 0.001-0.003 parts by weight, Bacillus coagulans 0.001-0.003 parts by weight, fructooligosaccharide It is more preferable to include 0.001-0.003 parts by weight, 0.001-0.003 parts by weight of yucca root extract, 1.7-2.1 parts by weight of sorbitol, 0.0003-0.0007 parts by weight of vitamin A, 0.0003-0.0007 parts by weight of vitamin B12, 0.0003-0.0007 parts by weight of vitamin D, 0.0003-0.0007 parts by weight of vitamin E, 0.0008-0.0012 parts by weight of zinc, 0.0008-0.0012 parts by weight of manganese, 0.0008-0.0012 parts by weight of DL-methionine, 0.0008-0.0012 parts by weight of L-lysine, and 0.001-0.003 parts by weight of potassium sorbate.

[0039] Rice flour, sweet potato starch, and cellulose are natural dietary fibers and binders that maintain the physical form of gum, aid digestion, and support gut health.

[0040] Glycerin, propylene glycol, and sorbitol function as moisturizers and emollients, making the gum softer so that it is easier for pets to chew, and they have a positive effect on oral mucosal health by reducing dryness in the mouth.

[0041] Hyaluronic acid improves the health of the oral mucosa and gums and helps alleviate inflammation through its excellent moisturizing properties and tissue regeneration-promoting effects.

[0042] Flaxseed oil and salmon oil are rich in omega-3 fatty acids and anti-inflammatory components, improving skin and hair health and contributing to the alleviation of inflammation in the mouth and skin through their anti-inflammatory effects.

[0043] Fucoidan is a polysaccharide derived from seaweed that exhibits immune-modulating, anti-inflammatory, and antiviral effects, helping to improve systemic immunity and maintain oral health.

[0044] Astaxanthin is a powerful antioxidant that prevents cell damage and contributes to anti-aging, playing a positive role in maintaining the health and shine of the skin and hair.

[0045] Bacillus coagulans is a probiotic that promotes the growth of beneficial bacteria in the intestines and contributes to improving digestive function and boosting immunity.

[0046] Fructooligosaccharides are prebiotics that support the balance of gut microorganisms, helping to improve digestive health and strengthen the immune system.

[0047] Yucca root extract reduces bad breath by breaking down odor-causing substances in the mouth and supports oral tissue health through its anti-inflammatory action.

[0048] Vitamins A, B12, D, E, zinc, and manganese play a key role in strengthening overall immune function, promoting cell regeneration, maintaining skin and hair health, and protecting bone and joint health.

[0049] DL-methionine and L-lysine are essential amino acids involved in protein synthesis and contribute to maintaining the health of skin and muscle tissue.

[0050] Potassium sorbate is a safe natural preservative that increases the storage stability of gum and inhibits the growth of microorganisms, thereby maintaining product freshness.

[0051] In addition, it is preferable that the above pet chew further include 1-5 parts by weight of fermented functional powder, and more preferable that it further include 2-4 parts by weight.

[0052] The above-mentioned fermented functional powder applies an extract prepared by fermenting a complex of graviola leaves, prickly pear stems, and true needle mushrooms mixed in a weight ratio of 1:1:1 and then extracting it.

[0053] In addition, the above-mentioned fermented functional powder is,

[0054] A method for preparing a product is applied by performing the following steps: adding 48-52 parts by weight of a complex mixture of graviola leaves, prickly pear stems, and *Cham-ba-neul-mushroom* in a weight ratio of 1:1:1 and 48-52 parts by weight of purified water, and adding 0.8-1.2 parts by weight of a mixed strain of Aspergillus oryzae and Sporobolomyces papyii in a weight ratio of 1:1, followed by fermentation at a temperature of 33-37℃ for 2-4 days; after fermentation, adding 38-42 parts by weight of hexane and extracting at a temperature of 88-92℃ for 11-13 hours to prepare an extract; and concentrating the extract under reduced pressure and freeze-drying it.

[0055] Graviola (Annona muricata) belongs to the Annonaceae family, which comprises approximately 2,300 species, of which about 130 genera are widely distributed in the United States, Africa, Asia, Brazil, and other regions. The trees reach a height of 6–7 m. The leaves are egg-shaped or oblong, measuring 7.5–15 cm in length; they have pointed tips and wedge-shaped petioles. The leaves are smooth and glossy, with short petioles. The flowers are strongly fragrant and 0.5–2.5 cm in length; the sepals are detached from the petals and measure 3–5 cm. The petals are compound, consisting of six leaflets; the outer petals are thick, leathery, and broadly egg-shaped with pointed tips, covered with soft hairs.

[0056] Cheonnyeoncho (Opuntia humifusa) is a plant belonging to the genus Opuntia in the subfamily Opuntioideae. It is a native Korean cactus with a tenacious vitality that allows it to survive even in open winter fields by reducing its moisture content to less than half, even in severe cold temperatures of -20°C. It is also known as the "Palm Cactus" due to its resemblance to a palm. Excluding the thorns, the stems, fruits, roots, and flowers each contain specific nutrients and pigments, making them highly valuable for commercial use and allowing for the development of products that leverage their distinct characteristics. In particular, Cheonnyeoncho contains various nutrients important to the human body, including phenolic compounds, flavonoids, dietary fiber, vitamin C, calcium, minerals, amino acids, and complex polysaccharides; among these, it contains significantly higher amounts of dietary fiber and calcium compared to other plants.

[0057] Mycoleptodonoides aitchisonii, also known as the needle mushroom, is a type of edible mushroom with a fruity scent. Its fruiting body is sessile and has a soft, fleshy texture that hardens when dry. The cap is fan-shaped or spatula-shaped and grows in large numbers; the surface is smooth, white or pale yellow, and the margin is tooth-shaped. The hyphae are of two types: thick and thin. The hymenium on the underside is needle-shaped, with pointed needles measuring 3 to 10 mm in length that turn yellowish-orange when dry. Mycoleptodonoides aitchisonii is expected to possess excellent nutritional value along with pharmacological effects such as blood pressure lowering, blood sugar lowering, brain function improvement, anticancer, and antidiabetic effects.

[0058] The above-mentioned fermented functional powder is extracted after fermenting graviola leaves, prickly pear stems, and true needle mushrooms, but the efficacy of the provided extract is further enhanced by applying a mixed strain of Aspergillus oryzae and Sporobolomyces papyii in a weight ratio of 1:1 to further increase the content of functional components in the raw material.

[0060] In addition, the present invention

[0061] A step of preparing a fermented functional powder comprising: adding 48-52 parts by weight of a complex of graviola leaves, prickly pear stems, and *Cham-ba-neul-mushroom* mixed in a weight ratio of 1:1:1 and 48-52 parts by weight of purified water, and adding 0.8-1.2 parts by weight of a mixed strain of Aspergillus oryzae and Sporobolomyces papyii mixed in a weight ratio of 1:1, followed by fermentation at a temperature of 33-37℃ for 2-4 days; a step of preparing an extract by adding 38-42 parts by weight of hexane after fermentation and extracting at a temperature of 88-92℃ for 11-13 hours; and a step of concentrating the extract under reduced pressure and freeze-drying it.

[0062] 1-5 parts by weight of the above-mentioned fermented functional powder, 1-5 parts by weight of low molecular weight fish collagen with a molecular weight of 10-1,000 Da, 4-8 parts by weight of shrimp hydrolysate with a molecular weight of 1-500 Da, 0.001-0.003 parts by weight of propolis, 0.22-0.26 parts by weight of spirulina, 0.832-0.836 parts by weight of hexametaphosphate (SHMP), 56-60 parts by weight of rice flour, 6-10 parts by weight of cellulose, 6-10 parts by weight of glycerin, 6-10 parts by weight of propylene glycol, 4-8 parts by weight of sweet potato starch, 0.001-0.003 parts by weight of hyaluronic acid, 0.001-0.003 parts by weight of flaxseed oil, 0.001-0.003 parts by weight of salmon oil, and fucoidan. 0.001-0.003 parts by weight, astaxanthin 0.001-0.003 parts by weight, Bacillus coagulans 0.001-0.003 parts by weight, fructooligosaccharide 0.001-0.003 parts by weight, yucca root extract 0.001-0.003 parts by weight, sorbitol 1.7-2.1 parts by weight, vitamin A 0.0003-0.0007 parts by weight, vitamin B12 0.0003-0.0007 parts by weight, vitamin D 0.0003-0.0007 parts by weight, vitamin E 0.0003-0.0007 parts by weight, zinc 0.0008-0.0012 parts by weight, manganese 0.0008-0.0012 parts by weight, DL-methionine A step of mixing 0.0008-0.0012 parts by weight of L-lysine, 0.0008-0.0012 parts by weight of L-lysine, and 0.001-0.003 parts by weight of potassium sorbate and feeding the mixture into an extruder; and

[0063] A method for manufacturing a pet chewing gum is provided, comprising the steps of extruding with an extruder, cooling, and cutting.

[0064] The pet gum according to the present invention is a gum comprising low molecular weight fish collagen with a molecular weight of 10-1,000 Da, shrimp hydrolysate with a molecular weight of 1-500 Da, propolis, spirulina, and hexametaphosphate (SHMP). By applying low molecular weight fish collagen with a molecular weight of 1,000 Da or less and hydrolyzed protein with a molecular weight of 500 Da or less, nutrients are broken down into very small sizes to minimize immune responses, prevent dietary allergies to the substances applied as protein sources, and promote oral health by applying propolis, spirulina, and hexametaphosphate.

[0066] The present invention will be explained in more detail below by the following examples.

[0067] However, the following examples are merely illustrative of the content of the invention, and the scope of the invention is not limited by the examples and experimental examples.

[0068] <Preparation Example 1> Preparation of Fermented Functional Powder-1

[0069] 50 parts by weight of a complex mixture of graviola leaves, Opuntia ficus-indica stems, and Pterocarya persica mushrooms in a weight ratio of 1:1:1 and 50 parts by weight of purified water were added, and 1 part by weight of a Lactobacillus plantarum strain was added, followed by fermentation at a temperature of 35°C for 3 days. After fermentation, 40 parts by weight of hexane were added, and an extract was prepared by extracting at a temperature of 90°C for 12 hours. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented functional powder.

[0070] <Preparation Example 2> Preparation of Fermented Functional Powder-2

[0071] 50 parts by weight of a complex mixture of graviola leaves, Opuntia ficus-indica stems, and Pterocarya persica mushrooms in a weight ratio of 1:1:1 and 50 parts by weight of purified water were added, followed by the addition of 1 part by weight of a Bacillus subtilis strain, and fermentation was carried out at a temperature of 35°C for 3 days. After fermentation, 40 parts by weight of hexane were added, and an extract was prepared by extracting at a temperature of 90°C for 12 hours. The extract was concentrated under reduced pressure and freeze-dried to produce a fermented functional powder.

[0072] <Preparation Example 3> Preparation of Fermented Functional Powder-3

[0073] 50 parts by weight of a complex mixture of graviola leaves, Opuntia ficus-indica stems, and Pterocarya persica mushrooms in a weight ratio of 1:1:1 and 50 parts by weight of purified water were added, and 1 part by weight of Aspergillus oryzae strain was added, followed by fermentation at a temperature of 35°C for 3 days. After fermentation, 40 parts by weight of hexane were added, and an extract was prepared by extracting at a temperature of 90°C for 12 hours. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented functional powder.

[0074] <Preparation Example 4> Preparation of Fermented Functional Powder-4

[0075] 50 parts by weight of a complex mixture of graviola leaves, Opuntia ficus-indica stems, and Pterocarya persica mushrooms in a weight ratio of 1:1:1 and 50 parts by weight of purified water were added, and 1 part by weight of Sporobolomyces papyii strain was added, followed by fermentation at 35°C for 3 days. After fermentation, 40 parts by weight of hexane were added, and an extract was prepared by extracting at 90°C for 12 hours. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented functional powder.

[0076] <Preparation Example 5> Preparation of Fermented Functional Powder-5

[0077] 50 parts by weight of a complex mixture of graviola leaves, Opuntia ficus-indica stems, and Pterocarya persica mushrooms in a weight ratio of 1:1:1 and 50 parts by weight of purified water were added, and 1 part by weight of a mixed strain of Aspergillus oryzae and Sporobolomyces papyii in a weight ratio of 1:1 was added, followed by fermentation at a temperature of 35°C for 3 days. After fermentation, 40 parts by weight of hexane were added, and an extract was prepared by extracting at a temperature of 90°C for 12 hours. The extract was concentrated under reduced pressure and freeze-dried to prepare a fermented functional powder.

[0078] <Experimental Example 1> Component Analysis

[0079] To analyze the GABA content of the fermented functional powder prepared in Preparation Examples 1-5 above, analysis was performed using a 6410A triple quadrupole mass spectroscopy (Agilent, USA) coupled with a 1200 series LC (Agilent, USA), and the results are shown in Table 1 below.

[0080] GABA content (mg / g) Preparation Example 1 8.2 Preparation Example 2 9.8 Preparation Example 3 10.1 Preparation Example 4 10.4 Preparation Example 5 13.2

[0081] As shown in Table 1 above, it was confirmed that the fermented functional powder according to the present invention has a high GABA content.

[0082] <Experimental Example 2> Analysis of Functionality for Improving Joint Health

[0083] Animal experiments were conducted to confirm the joint health efficacy of the fermented functional powder prepared in Preparation Examples 3-5 above. 5-month-old male rats (Orient Bio Co., Ltd.) were acclimatized for one week and then used, and were provided with free feed and drinking water. The fermented functional powder prepared in Preparation Examples 3-5 above was prepared to the following concentrations and administered orally daily. The test groups were classified into a control group (untreated group) and a group administered the fermented functional powder prepared in Preparation Examples 3-5, and each composition was diluted to 200 mg / kg and administered. The drug was administered orally to each group (5 animals) for 6 weeks. The percentage of bone volume (% bone volume; BV / TV), trabecular number (Tbn), trabecular thickness (TbTh), trabecular separation (TbSp; average distance between trabeculars), and structure model index (SMI; indicator of trabecular structure) relative to total tissue volume within the region of interest of the distal leg joints of male rats in which the experiment was performed were analyzed using a statistical program (Microcal OriginPro 80, Originlab, Northampton, MA, USA), and the results are shown in Table 2 below.

[0084] control group Preparation Example 3 Preparation Example 4 Preparation Example 5 Bone volume percentage (%) 11.23 15.32 16.55 18.00 Number of bone marrow ( / mm) 0.35 0.40 0.44 0.45 Thickness of bone marrow (mm) 0.60 0.59 0.59 0.60 Spacing of bone trabeculae (mm) 2.05 1.80 1.72 1.55 Structural form index 1.80 2.05 2.20 2.36

[0085] As shown in Table 2 above, it was confirmed that when the fermented functional powder according to the present invention is applied, the bone volume ratio is improved and the number of bone stratums increases.

Claims

Claim 1 1-5 parts by weight of low molecular weight fish collagen with a molecular weight of 10-1,000 Da, 4-8 parts by weight of shrimp hydrolysate with a molecular weight of 1-500 Da, 1-5 parts by weight of fermented functional powder, 0.001-0.003 parts by weight of propolis, 0.22-0.26 parts by weight of spirulina, 0.832-0.836 parts by weight of hexametaphosphate (SHMP), 56-60 parts by weight of rice flour, 6-10 parts by weight of cellulose, 6-10 parts by weight of glycerin, 6-10 parts by weight of propylene glycol, 4-8 parts by weight of sweet potato starch, 0.001-0.003 parts by weight of hyaluronic acid, 0.001-0.003 parts by weight of flaxseed oil, 0.001-0.003 parts by weight of salmon oil, and fucoidan. 0.001-0.003 parts by weight, astaxanthin 0.001-0.003 parts by weight, Bacillus coagulans 0.001-0.003 parts by weight, fructooligosaccharide 0.001-0.003 parts by weight, yucca root extract 0.001-0.003 parts by weight, sorbitol 1.7-2.1 parts by weight, vitamin A 0.0003-0.0007 parts by weight, vitamin B12 0.0003-0.0007 parts by weight, vitamin D 0.0003-0.0007 parts by weight, vitamin E 0.0003-0.0007 parts by weight, zinc 0.0008-0.0012 parts by weight, manganese 0.0008-0.0012 parts by weight, DL-methionine A pet chew comprising 0.0008-0.0012 parts by weight, 0.0008-0.0012 parts by weight of L-lysine, and 0.001-0.003 parts by weight of potassium sorbate, wherein the fermented functional powder is an extract prepared by fermenting and extracting a complex of graviola leaves, prickly pear stems, and *Cham-ba-neul-mushroom* mixed in a weight ratio of 1:1:

1. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 delete

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