Complex aroma healing serum composition containing fermented oil and method for producing the same

Abstract

In a complex aroma healing serum composition comprising a fermented oil according to the present disclosure for achieving the aforementioned technical objectives, the composition comprises fermented hemp seed oil biotransformed through a lactic acid bacteria fermentation process as a base oil and may additionally comprise a mixture of natural essential oils. Furthermore, the fermented hemp seed oil may be fermented using lactic acid bacteria of the genus Lactobacillus.

Description

Technology Field

[65535] The present invention relates to the field of cosmetic compositions, and specifically, to a complex aroma healing serum composition comprising a plant-based fermented oil, particularly fermented hemp seed oil, bio-converted through a fermentation process as a base oil, and a combination of multiple types of naturally derived essential aroma oils, and a method for manufacturing the same. Background Technology Unless otherwise indicated in this specification, the contents described in this section are not prior art for the claims of this application, and are not to be recognized as prior art simply because they are included in this section. Recently, there has been an increasing demand among consumers for multi-purpose premium oil serum products that go beyond the simple use of skincare products to simultaneously satisfy the needs of skin function restoration, stress relief, and psychological healing experiences. In particular, as numerous research results have reported that natural oil materials based on unsaturated fatty acids provide beneficial effects such as anti-inflammatory, antioxidant, skin barrier improvement, and cell regeneration, interest in high-quality skincare compositions based on natural plant oils and fermented milks is continuously expanding in the cosmetics field. Hemp seed oil contains large amounts of polyunsaturated fatty acids, such as linoleic acid, alpha-linolenic acid, and gamma-linolenic acid, and is known to have excellent antioxidant and anti-inflammatory effects. In particular, functional enhancements such as reduced surface tension, increased emulsifying activity, improved absorption, and increased bioactive components of hemp seed oil can be achieved through fermentation processes using lactic acid bacteria like Lactobacillus. Furthermore, fermented oils exhibit high oxidative stability, making them suitable for long-term preservation and application in various cosmetic formulations. This fermented oil-based cosmetic technology is evolving to address issues such as substrate instability, stickiness, and low absorption compared to existing natural extract oil-based cosmetics. Meanwhile, in the field of aromatherapy, there is active development of targeted serum formulations for skin soothing, revitalization, anti-inflammation, regeneration promotion, and brightening by complexly blending premium essential oils, each possessing unique psychological, emotional, and skin functions. However, conventional complex aroma compositions have suffered from insufficient efficacy and skin barrier delivery capabilities upon actual application because the biological functions or absorption enhancements of the base oils themselves are not sufficient.Therefore, there is an increasing need to provide a single composition that comprehensively integrates the advantages of fermented oils, such as stability, absorption, and antioxidant activity, with the skin improvement functions and psychological healing effects of premium essential oils selected for specific purposes. Prior art literature 1. Korean Patent Registration No. 10-2316168 (Oct. 18, 2021) 2. Korean Patent Registration No. 10-1454970 (Oct. 20, 2014) The problem to be solved The purpose of the embodiments disclosed in this disclosure is to provide a complex aroma healing serum composition and manufacturing technology by applying fermented hemp seed oil as a base through a fermentation process-based bioconversion and selectively combining premium natural essential oils therewith. Meanwhile, the technical problems of the present invention are not limited to those mentioned above, and other unmentioned technical problems will be clearly understood by a person skilled in the art from the description below. means of solving the problem In a complex aroma healing serum composition comprising fermented oil according to the present disclosure for achieving the technical problem described above, the composition comprises fermented hemp seed oil biotransformed through a lactic acid bacteria fermentation process as a base oil, and further comprises a natural essential oil mixture, wherein the surface tension of the fermented hemp seed oil may be reduced compared to non-fermented hemp seed oil. Additionally, the fermented hemp seed oil may be fermented using lactic acid bacteria of the genus Lactobacillus. Furthermore, the natural essential oil mixture may include at least one of lavender oil, neroli oil, sandalwood oil, orange oil, lemon oil, grapefruit oil, and geranium oil. Additionally, the natural essential oil mixture may further include at least one of tea tree oil, chamomile oil, helichrysum oil, myrrh oil, and frankincense oil. A method for preparing a complex aroma healing serum composition comprising fermented oil according to another embodiment comprises: (a) a step of preparing a hemp seed oil raw material; (b) a step of fermenting the hemp seed oil using lactic acid bacteria of the genus Lactobacillus to obtain fermented hemp seed oil with reduced surface tension; (c) a step of adding an aroma oil blend mixture combining natural essential oils to the fermented hemp seed oil; (d) a step of uniformly mixing and aging the mixture to complete a fermented oil-based complex aroma healing serum composition; may be included. Additionally, the fermentation step (b) may include a step of fermenting for 8 to 72 hours in a pH range of 4.0 to 6.8. Additionally, the natural essential oil added in step (c) may include a step of selecting and blending from aroma oil combinations pre-blended by functional group. Effects of the invention delete Brief explanation of the drawing FIG. 1 is a diagram illustrating an example of the manufacturing flow of a fermented oil (BST - Ferment Oil 2.0) applied to a complex aroma healing serum composition containing fermented oil according to the present invention. FIG. 2 is a diagram showing the manufacturing process of a complex aroma healing serum composition containing fermented oil according to an example. FIG. 4 is a diagram showing an example of the fatty acid composition in hemp seed oil. Specific details for implementing the invention Various embodiments of the present disclosure are described below in conjunction with the accompanying drawings. Various embodiments of the present disclosure may be subject to various modifications and may have various forms; specific embodiments are illustrated in the drawings and described in detail. However, it should be understood that the various embodiments of the present disclosure are not limited to specific forms and include all modifications and / or equivalents and substitutions that fall within the spirit and scope of the various embodiments of the present disclosure. In relation to the description of the drawings, similar reference numerals have been used for similar components. In various embodiments of the present disclosure, terms such as “comprising” or “having” are intended to indicate the presence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should not be understood as precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof. In various embodiments of the present disclosure, expressions such as “or” include any and all combinations of the words listed together. For example, “A or B” may include A, may include B, or may include both A and B. Expressions such as “first,” “second,” “first,” or “second” used in various embodiments of the present disclosure may modify various components of various embodiments, but do not limit such components. For example, such expressions do not limit the order and / or importance of such components and may be used to distinguish one component from another. When it is mentioned that a component is “connected” or “joined” to another component, it should be understood that the component may be directly connected or joined to the other component, but that there may also be a new component between the component and the other component.Terms such as those defined in commonly used dictionaries should be interpreted as having meanings consistent with their meanings in the context of the relevant technology, and should not be interpreted in an ideal or overly formal sense unless explicitly defined in the various embodiments of the present disclosure. Hereinafter, embodiments of the present disclosure will be described in detail with reference to the attached drawings. FIG. 1 is a diagram illustrating an example of the manufacturing flow of a fermented oil (BST - Ferment Oil 2.0) applied to a complex aroma healing serum composition containing fermented oil according to the present invention. As shown in FIG. 1, natural oil is first provided to a bioconversion process, impurities are removed through a purification step after the process, and then a fermentation process is performed to produce fermented oil. Fermented oil (BST - Ferment Oil 2.0) is obtained through the bioconversion process and the purification step. FIG. 2 is a diagram showing the manufacturing process of a complex aroma healing serum composition containing fermented oil according to an embodiment. Referring to FIG. 2, in step S110, a hemp seed oil raw material is prepared. The hemp seed oil raw material may be natural hemp seed oil extracted from hulled plant-based hemp seeds. The hemp seed oil raw material may be secured as a base material to be input into the manufacturing process. The hemp seed oil raw material may be prepared to maintain a state suitable for fermentation pretreatment. A state suitable for fermentation pretreatment may be a condition in which the quality of the hemp seed oil raw material is controlled before being input into the fermentation process. Quality control of the hemp seed oil raw material may be performed by pre-checking the acid value, moisture content, and unsaturated fatty acid composition ratio. The acid value may be a value indicating the degree of acidic components contained in the hemp seed oil raw material. The moisture content may be the amount of water contained in the hemp seed oil raw material. The unsaturated fatty acid composition ratio may be the ratio of unsaturated fatty acid components among the fatty acid components of the hemp seed oil raw material.Based on the preliminary verification of acid value, moisture content, and composition ratio of unsaturated fatty acids, the hemp seed oil raw material can be prepared within a quality standard range suitable for input into the fermentation process. The quality standard range may be a pre-set allowable range for input into the fermentation process. The hemp seed oil raw material prepared within the quality standard range may be input into fermentation using lactic acid bacteria of the genus Lactobacillus in a subsequent step. In step S120, fermented hemp seed oil can be obtained by fermenting hemp seed oil using lactic acid bacteria of the genus Lactobacillus. Lactic acid bacteria of the genus Lactobacillus may refer to lactic acid bacteria belonging to the genus Lactobacillus. Fermentation may refer to a process in which hemp seed oil is treated in a fermentation environment containing lactic acid bacteria of the genus Lactobacillus. Fermented hemp seed oil may refer to hemp seed oil that has undergone the fermentation process. The fermentation process may be performed while the hemp seed oil and lactic acid bacteria of the genus Lactobacillus are in contact. The manufacturing facility may introduce hemp seed oil into a container where a fermentation reaction can proceed. The manufacturing facility may provide lactic acid bacteria of the genus Lactobacillus to a fermentation environment where hemp seed oil is present, thereby allowing the hemp seed oil to come into contact with the lactic acid bacteria of the genus Lactobacillus. In the fermentation process, the pH may be set to 4.0 or higher and 6.8 or lower. The setting of the pH to 4.0 or higher and 6.8 or lower may be performed in a manner that maintains the pH of the fermentation environment to be 4.0 or higher and 6.8 or lower. The fermentation time may be set to 8 hours or higher and 72 hours or lower. The setting of 8 hours or higher and 72 hours or lower may be performed in a manner that controls the process so that the state in which the hemp seed oil comes into contact with the lactic acid bacteria of the genus Lactobacillus in the fermentation environment is maintained for 8 hours or more and terminates at 72 hours or less. After the fermentation process is performed within the set pH and set fermentation time ranges, the manufacturing facility may obtain the hemp seed oil that has undergone the fermentation process as fermented hemp seed oil.In an example of manufacturing fermented hemp seed oil, the hemp seed oil raw material may be prepared so as to be input into the fermentation process. Lactobacillus genus lactic acid bacteria may be prepared as a fermentation strain used in the fermentation process. The fermentation strain may include at least one of Lactobacillus plantarum KCTC3107, Lactobacillus plantarum KCTC3108, Lactobacillus brevis BHNLAB-128, and Lactobacillus paracasei BHNLAB-129. The fermentation strain may be used as a single strain. Multiple strains may be used together. The medium may be prepared as an aqueous medium for the culture and fermentation reaction of Lactobacillus genus lactic acid bacteria. The medium may be prepared with a composition containing a carbon source, a nitrogen source, and inorganic salts to enable the proliferation of Lactobacillus genus lactic acid bacteria. The medium may be prepared in a sterile state. Sterilization treatment may be performed to reduce the contamination of unintended microorganisms within the culture medium. The starter culture solution may be prepared as a culture medium in which a fermentation strain has been cultured. The starter culture solution may be prepared as a liquid containing bacterial cells by culturing the fermentation strain in the culture medium for a certain period of time. The starter culture solution may be measured into a predetermined volume so that it can be introduced into the fermentation process. The fermentation vessel may be prepared as a vessel in which the fermentation process is performed. The fermentation vessel may include an internal space into which the culture medium, starter culture solution, and hemp seed oil raw material can be introduced. The fermentation vessel may include a structure in which internal pressure and internal gas atmosphere can be controlled. The fermentation vessel may include a stirring configuration in which internal stirring can be performed. The stirring configuration may include a configuration that forms an internal flow to allow the liquid phase and the oil phase to come into contact. In the fermentation process, the culture medium may be introduced into the fermentation vessel. The starter culture solution may be introduced into the fermentation vessel. The hemp seed oil raw material may be introduced into the fermentation vessel.The hemp seed oil raw material may be introduced into the fermentation vessel to come into contact with the culture medium and inoculum. The state in which the hemp seed oil raw material comes into contact with the culture medium and inoculum may be formed by driving a stirring configuration. Under fermentation conditions, the pH inside the fermentation vessel may be set to 4.0 or higher and 6.8 or lower. The setting of pH 4.0 or higher and 6.8 or lower may include a state in which the pH inside the fermentation vessel is controlled to be 4.0 or higher and 6.8 or lower. The fermentation time may be set to 8 hours or higher and 72 hours or lower. The setting of 8 hours or higher and 72 hours or lower may include a state in which the state in which the hemp seed oil raw material comes into contact with the fermentation strain inside the fermentation vessel is maintained for 8 hours or more and ends at 72 hours or less. After the fermentation process is completed, the fermentation vessel may contain the hemp seed oil that has undergone the fermentation process. The hemp seed oil that has undergone the fermentation process may be obtained as fermented hemp seed oil. The acquisition of fermented hemp seed oil may include a process of separating oil components from a mixture inside a fermentation vessel. The separation of oil components may be performed through at least one of static separation, filtration separation, and centrifugation. The separated oil components may be obtained as fermented hemp seed oil. In step S130, a natural essential oil mixture may be prepared. The natural essential oil mixture may be an oil mixture comprising two or more different types of essential oils among lavender oil, neroli oil, sandalwood oil, orange oil, lemon oil, grapefruit oil, geranium oil, tea tree oil, chamomile oil, helichrysum oil, myrrh oil, and frankincense oil. Two or more different types of essential oils may mean a composition in which at least two types of essential oils with different names are selected so that essential oils with the same name do not overlap. The content of each essential oil may be defined in parts by weight. A part by weight may mean the relative mass of each component relative to a reference total amount.The content standard for the natural essential oil mixture can be set to a total amount of 100 parts by weight of the natural essential oil mixture. A total amount of 100 parts by weight of the natural essential oil mixture may refer to a value obtained by normalizing the sum of the weights of the selected essential oils constituting the natural essential oil mixture to 100 parts by weight. In this standard, the sum of the weight parts of each selected essential oil may be 100 parts by weight. Each selected essential oil may be included in a range of 0.1 parts by weight or more and 99.9 parts by weight or less, based on a total amount of 100 parts by weight. The manufacturing equipment may select two or more different types of essential oils. The manufacturing equipment may set a target value by weight for each selected essential oil. The target value by weight may be a value assigned to each essential oil based on a total amount of 100 parts by weight of the natural essential oil mixture. The manufacturing equipment may extract each selected essential oil from an individual container and place it into a mixing container. The manufacturing equipment may use a weighing device to weigh the amount of each essential oil extracted so that it corresponds to the target value by weight. The weighing device may include at least one of a scale, a load cell, and a mass flow meter. The manufacturing facility may mix the essential oils introduced into the mixing container to form a natural essential oil mixture. Mixing may be performed so that the composition between the essential oils becomes uniform within the mixing container. The manufacturing facility may use the prepared natural essential oil mixture as a first mixture. In step S140, fermented hemp seed oil may be introduced as a base solution into a stirring tank including a stirrer, and the natural essential oil mixture may be introduced as a first mixture to form a second mixture. The stirring tank including a stirrer may be a tank including a stirrer to stir the liquid introduced into the tank. The base solution may refer to fermented hemp seed oil. The first mixture may refer to a natural essential oil mixture.The second mixture may refer to a mixed state in which the base solution and the first mixture coexist inside a stirring tank containing a stirrer. The process of introducing the base solution into the stirring tank containing the stirrer may be performed with the container storing the base solution and the stirring tank containing the stirrer connected by a fluid transfer path. The fluid transfer path may include at least one of piping, hoses, valves, and pumps. The introduction of the base solution may be performed so that the base solution moves into the interior of the stirring tank containing the stirrer through the fluid transfer path. The amount of the base solution introduced may be metered and introduced according to the input amount set in the manufacturing process. The process of introducing the first mixture into the stirring tank containing the stirrer may be performed with the container storing the first mixture and the stirring tank containing the stirrer connected by a fluid transfer path. The introduction of the first mixture may be performed so that the first mixture moves into the interior of the stirring tank containing the stirrer through the fluid transfer path. The inflow amount of the first mixture may be metered and introduced according to the input amount set in the manufacturing process. In the process of forming the second mixture, the stirrer may be driven to mix the base solution and the first mixture inside a stirring tank containing the stirrer. The driving of the stirrer may be performed by at least one of rotation or reciprocating motion of the stirrer. While the base solution and the first mixture are introduced into the stirring tank containing the stirrer and the stirrer is driven, a second mixture in which the base solution and the first mixture coexist may be formed inside the stirring tank containing the stirrer. The mixing conditions in step S140 may be defined so that the fermented hemp seed oil and the natural essential oil mixture are formed as the second mixture within the same stirring tank. The fermented hemp seed oil may first be introduced into the stirring tank as the base solution. The natural essential oil mixture may be additionally introduced into the stirring tank into which the base solution has been introduced as the first mixture.The inflow order of the base solution and the first mixture can be set so that the base solution is introduced first, followed by the first mixture, or so that the inflow of the base solution and the inflow of the first mixture overlap in time. The mixing ratio can be defined in parts by weight. The standard for the mixing ratio can be set to 100 parts by weight of the base solution. The natural essential oil mixture can be set to be introduced in an amount of 0.1 parts by weight or more and 30 parts by weight or less, based on 100 parts by weight of the base solution. The setting value of the mixing ratio can be stored as a setting value of the manufacturing equipment, and according to the setting value, the inflow amounts of the base solution and the first mixture can be measured and introduced into the mixing tank. Measurement can be performed based on mass measurements. The stirring conditions can be defined as the operating conditions of the stirrer. The stirrer can be operated after the base solution is introduced into the mixing tank, or before the inflow of the first mixture begins, or simultaneously with the inflow of the first mixture. The stirring speed can be set to 100 rpm or more and 500 rpm or less. The stirring time can be set from the start of the inflow of the first mixture to a range of 5 minutes or more and 60 minutes or less. The stirring speed and stirring time can be controlled by fixed conditions by the manufacturing equipment. Mixing conditions in a reduced pressure environment can be defined as conditions in which the internal pressure of the stirring tank is controlled based on gauge pressure. The inside of the stirring tank can be controlled to a reduced pressure environment while the second mixture is being formed. The gauge pressure inside the stirring tank can be set to -0.09 MPa or more and -0.08 MPa or less. The reduction of pressure can be performed by driving a pressure reduction device to discharge gas inside the stirring tank. The pressure reduction device can be driven so that the internal pressure of the stirring tank is maintained within the set gauge pressure range. The application period of the pressure reduction can be set from before the start of the inflow of the first mixture to the end of stirring, or it can be set from the start of the inflow of the first mixture to the end of stirring. In step S150, the second mixture can be uniformly mixed and aged to complete the fermented oil-based complex aroma healing serum composition.The second mixture may refer to a mixture existing inside a stirring tank containing a stirrer. Uniform mixing may refer to a process of driving the stirrer so that the mixture of fermented hemp seed oil and natural essential oil constituting the second mixture is mixed without deviation inside the stirring tank. Aging may refer to a process in which the second mixture, after uniform mixing, is stored inside the stirring tank for a set period of time. Aging conditions may be defined as the conditions under which the second mixture is maintained inside the stirring tank after the stirring ends. The aging time may be set to between 1 hour and 72 hours. The aging temperature may be set to between 10°C and 30°C. During aging, the stirrer may be kept in a stationary state, or it may be set to operate intermittently at a low speed during aging. At the end of aging, the second mixture inside the stirring tank may be extracted as a fermented oil-based complex aroma healing serum composition. During the uniform mixing process, the stirrer may be driven to mix the second mixture inside the stirring tank. The drive of the stirrer can be performed by the rotational movement of the stirrer. The drive of the stirrer can be performed so that the second mixture moves circulatingly within the stirring tank. The circulation of the second mixture can be performed in such a way that flow occurs around the stirrer and the second mixture moves dispersed throughout the entire area inside the stirring tank. The uniform mixing process can be performed in such a way that the second mixture remains in a mixed state while the stirrer is driven. During the aging process, the second mixture, after uniform mixing, can be stored inside the stirring tank for a set period of time. Storage can be performed in such a way that the second mixture remains inside the stirring tank. During storage, the temperature of the second mixture can be maintained at a temperature set by the temperature control of the manufacturing equipment. After the set period of time has elapsed, the second mixture existing inside the stirring tank can be completed as a fermented oil-based complex aroma healing serum composition.A reduced pressure environment may refer to a state where the pressure inside the mixing tank is lower than atmospheric pressure. Reduced pressure may refer to the pressure formed inside the mixing tank in a reduced pressure environment. Gauge pressure may refer to pressure calculated based on atmospheric pressure. A setting of reduced pressure between -0.09 MPa and -0.08 MPa may mean that the gauge pressure inside the mixing tank is controlled to be between -0.09 MPa and -0.08 MPa. The second mixture may refer to a mixture in which the base solution and the first mixture coexist inside the mixing tank. The stirrer may refer to a drive unit driven to agitate the liquid phase inside the mixing tank. The step of forming the second mixture may be performed in a reduced pressure environment. The manufacturing equipment may perform reduced pressure so that the pressure inside the mixing tank is between -0.09 MPa and -0.08 MPa. The performance of reduced pressure may be carried out by driving a pressure reduction device connected to the mixing tank so that the internal space of the mixing tank is maintained within the reduced pressure range. The pressure reduction device may be a device configured to reduce internal pressure by discharging gas from the internal space of the mixing tank. The manufacturing facility may operate the stirrer while a reduced pressure environment is formed. The operation of the stirrer may be performed to agitate the second mixture inside the mixing tank. Agitation may be performed in such a manner that the second mixture moves and mixes within the mixing tank by the operation of the stirrer. The manufacturing facility may operate the stirrer while maintaining the reduced pressure environment to agitate the second mixture in the reduced pressure environment. The mixing tank used in the process of forming the second mixture may include a tank body and an inlet. The tank body may be a container that forms an internal space. The mixing tank may include a stirrer to agitate the liquid present in the internal space of the tank body. The stirrer may include at least one of a rotating member, an impeller, and a stirring shaft that is driven to generate flow in the liquid within the internal space of the tank body.The base solution may be a solution that flows into the stirring tank and undergoes processing inside the stirring tank. The first mixture may be a mixture that flows into the stirring tank together with the base solution and is mixed with the base solution. The second mixture may be a mixture in which the base solution and the first mixture are mixed inside the stirring tank. The second mixture may flow into the lower open area of ​​the inlet section while circulating inside the tank body due to the internal flow of the stirring tank. In this case, "inflow" may include not only inflow entering the tank from the outside, but also inflow in which the liquid phase inside the tank body moves into the inlet section through the lower open area of ​​the inlet section that communicates with the internal space of the tank body. The inlet section may extend upward from the center of the upper surface of the tank body. The center of the upper surface may be an area corresponding to the center on the upper surface of the tank body. The inlet section may be cylindrical in shape. A cylindrical shape may refer to a shape in which a circular cross-section is continuous with respect to a central axis. The outer upper end of the inlet section may be open. An open end may refer to an open end that communicates with the outside. The bottom of the inlet may be open to the interior of the stirring tank. Opening to the interior may mean that the bottom of the inlet is opened to communicate with the internal space of the tank body. Due to this structure, the basic solution and the first mixture may flow into the inlet through the outer upper opening and then into the internal space of the tank body through the bottom opening. Additionally, the second mixture inside the tank body may flow into the interior of the inlet through the bottom opening. The inlet may include a first filter screen, a rotating part, and a second filter screen. The first filter screen may be coupled to the central part of the inlet. Coupling to the central part may mean being fixedly coupled to an area corresponding to the central axis of the inlet. The first filter screen may be configured to filter out large particulate foreign substances with a particle diameter of 100 μm or more. Particulate foreign substances may be solid foreign substance components mixed in the liquid phase. Particle diameter may be a dimension indicating the particle size.Particle size of 100 μm or more may refer to particles with a size of 100 micrometers or more. Filtering may mean that particles larger than the said size are separated by the mesh structure of the first filter so that they do not pass through the first filter. The pivot part may be positioned at the bottom of the first filter. Positioning at the bottom may mean being positioned at a location lower in the direction of gravity than where the first filter is positioned. The pivot part may be rotatably coupled by a hinge axis with respect to the outer wall of the inlet. The outer wall of the inlet may refer to the outer surface of the cylindrical shape of the inlet. The hinge axis may be an axis member that provides the rotational center of the pivot part. Rotatably coupled may mean that the pivot part is coupled to rotate around the hinge axis. The pivot part may be rotated to at least one of an open position and a closed position. The closed position may be a position where the pivot part is positioned to cover the internal flow path of the inlet. The opening position may be a position where the rotating part is positioned to open the internal flow path of the inlet part. The second filter screen may be mounted on the upper surface of the rotating part. The upper surface may refer to a surface facing the upper side of the rotating part. The second filter screen may be configured to filter out fine particulate foreign substances with a particle size greater than 10 μm and less than 100 μm. A particle size greater than 10 μm and less than 100 μm may refer to particles with a size exceeding 10 micrometers and less than 100 micrometers. The second filter screen may be configured to be detachable by magnetic force. Detachment by magnetic force may refer to a coupling method in which a magnetic body or magnet is placed on at least one side of the rotating part or the second filter screen, so that the second filter screen is attached to the upper surface of the rotating part by magnetic force and can be separated by an external force. A vibration generating module may be coupled to the inner lower surface of the rotating part. The inner lower surface may refer to the inner lower side surface of the rotating part. The vibration generating module may include a driving part driven to generate vibration in the rotating part.The vibration generating module can be driven at a frequency of 50 Hz to 200 Hz and an output of 1 W to 10 W when the rotating part is in a closed position. A frequency of 50 Hz to 200 Hz may refer to a range in which the vibration is repeated at a cycle of 50 to 200 times per second. An output of 1 W to 10 W may refer to a range of power consumed or transmitted by the vibration generating module. The vibration generating module may be configured to transmit vibration to the entire second filter through the upper surface of the rotating part by driving the module. Transmission of vibration to the entire second filter may refer to a transmission state in which vibration is transmitted across the entire surface area of ​​the second filter, rather than being concentrated locally in a specific part of the second filter. The vibration generating module may be controlled by an interlock control unit to be driven only when the rotating part is in a closed position. The interlock control unit may include a control configuration that controls to allow or block the driving of the vibration generating module depending on whether the position state of the rotating part is in a closed position. The positional state of the rotating part may be a state value indicating the angle of the rotating part or whether the rotating part is closed. The interlock control unit may be configured to provide a driving signal to the vibration generating module when the rotating part is in the closed position, and to block the provision of a driving signal to the vibration generating module when the rotating part is not in the closed position. Experimental samples may be classified as follows. Non-fermented hemp seed oil may be hemp seed oil that has not undergone a lactic acid bacteria fermentation process. Fermented hemp seed oil may be oil that has undergone a fermentation process using lactic acid bacteria of the genus Lactobacillus. The pH of the fermentation process may be set to 4.0 or higher and 6.8 or lower. The fermentation time may be set to 8 hours or higher and 72 hours or lower.The complex aroma healing serum composition may be a composition formed by mixing fermented hemp seed oil with a natural essential oil mixture comprising two or more different types of essential oils selected from lavender oil, neroli oil, sandalwood oil, orange oil, lemon oil, grapefruit oil, geranium oil, tea tree oil, chamomile oil, helichrysum oil, myrrh oil, and frankincense oil. The content standard for the natural essential oil mixture may be defined based on a total amount of 100 parts by weight of the natural essential oil mixture. A total amount of 100 parts by weight of the natural essential oil mixture may refer to a value obtained by normalizing the sum of the weights of the selected essential oils to 100 parts by weight. In this standard, the sum of the weight parts of each selected essential oil may be 100 parts by weight. In the test example, the comparative group may be composed as follows. Comparative group 1 may be non-fermented hemp seed oil. Comparative group 2 may be fermented hemp seed oil. Comparison group 3 may be a composition containing non-fermented hemp seed oil and not containing a natural essential oil mixture. Example group 1 may be a complex aroma healing serum composition containing fermented hemp seed oil and a natural essential oil mixture. In the same test, each group may be evaluated under the same storage conditions and the same measurement conditions. A complex aroma healing serum (Example group 1) was prepared by mixing according to the method of the above example in the following compositional ratio. Specifically, based on the total weight of the composition (100 wt%), it was formulated to contain 95.0 wt% of fermented hemp seed oil as a base oil and 5.0 wt% of an essential oil mixture. At this time, the mixture of the seven types of natural essential oils (total 5.0 wt%) consisted of 1.0 wt% lavender oil, 0.5 wt% neroli oil, 0.5 wt% sandalwood oil, 0.8 wt% geranium oil, 0.8 wt% orange oil, 0.7 wt% lemon oil, and 0.7 wt% grapefruit oil. Subsequent Test Examples 1 to 6 were evaluated using the composition of Example Group 1.In Test Example 1, surface tension can be quantitatively measured using a surface tension measuring device. Surface tension may be a physical quantity corresponding to the surface free energy per unit length at the surface of a liquid. The unit of surface tension may be stated as mN / m. The measurement temperature may be set to 25°C. Before measurement, the sample may be stabilized at 25°C for at least 30 minutes. Each sample may be poured into a measuring cup of the same volume. Each sample may be measured repeatedly at least three times. The mean and standard deviation of the repeated measurements may be stated together. The rate of change in surface tension of fermented hemp seed oil relative to non-fermented hemp seed oil can be calculated and stated as a percentage (%). As a result of measuring the surface tension of non-fermented hemp seed oil and fermented hemp seed oil according to the present invention in accordance with Test Example 1, the average surface tension of non-fermented hemp seed oil (control group) was measured to be 31.2 ± 0.26 mN / m, while that of fermented hemp seed oil (example) was measured to be 27.4 ± 0.15 mN / m. In Test Example 2, the radical scavenging rate (%) can be quantitatively evaluated through a radical scavenging test. The radical scavenging test may be a test that calculates the degree to which a sample scavenges radicals in a reaction system where free radicals are present, based on the change in absorbance. The radical scavenging test may include at least one of the DPPH radical scavenging test and the ABTS radical scavenging test. The sample may be diluted to a preset concentration range. The concentration range can be set with at least five concentration points. Each concentration point can be measured repeatedly at least three times. Absorbance can be measured at the wavelength corresponding to each test. The radical scavenging rate (%) can be calculated based on the reduction in absorbance of the sample treatment group compared to the control group. A reaction curve can be constructed from the radical scavenging rate according to concentration. The IC50 can be calculated from the reaction curve. The IC50 may represent the sample concentration at which the radical scavenging rate becomes 50%. The IC50 may be stated for each of the non-fermented hemp seed oil, fermented hemp seed oil, and complex aroma healing serum composition.As a result of measuring the DPPH radical scavenging activity (IC50) of each sample according to Test Example 2, the IC50 value of non-fermented hemp seed oil was found to be 9.8 μg / mL, while the IC50 value of fermented hemp seed oil was measured to be 4.1 μg / mL. In Test Example 3, skin permeability can be quantitatively evaluated using a diffusion cell. Skin permeability evaluation can be performed by mounting skin tissue in a diffusion cell comprising a donor chamber and a receiver chamber, applying a sample to the donor chamber, and measuring the cumulative amount of the labeled component moving to the receiver chamber over time. The skin tissue may include at least one of human-derived skin, animal-derived skin, or an artificial skin model. The labeled component may be set as a quantifiable component among the components contained in fermented hemp seed oil. The labeled component may be set as at least one of linoleic acid, alpha-linolenic acid, and tocopherol. Quantification of the labeled component may be performed using a chromatography-based quantification method. The receiver chamber solution can be collected at preset time intervals. The collection times may include at least some of 0.5 hours, 1 hour, 2 hours, 4 hours, 8 hours, 12 hours, and 24 hours. The cumulative permeate can be calculated at each time point. The cumulative permeate can be calculated as the cumulative mass transferred per unit area. The unit may be stated as μg / cm². The flux can be calculated as the slope of the cumulative permeate versus time in the steady-state section. The unit may be stated as μg / cm² / h. The permeability coefficient can be calculated based on the flux and the donor concentration. Here, is time It can be the cumulative transmittance per unit area (μg / cm²). is time It may be the accumulated mass (μg) of the labeled component transferred from to the receiver chamber. can be the skin exposure area (cm²). can be the steady-state flux (μg / cm² / h). may be the initial concentration of the labeled component in the donor chamber (μg / cm³ or μg / mL). ε₀ may be the permeability coefficient (cm / h). As a result of measuring the cumulative permeability over 24 hours using an artificial skin model according to Test Example 3, the cumulative permeability of non-fermented hemp seed oil was 18.0 μg / cm², while that of fermented hemp seed oil was measured to be 33.1 μg / cm². In addition, the permeability coefficient (Kp) was also found to be approximately 1.8 times higher in fermented oil (0.0117 cm / h) compared to non-fermented oil (0.0063 cm / h). In Test Example 4, cell-based inflammatory response indicators may be quantitatively evaluated. Cells may include at least one of keratinocytes, macrophages, and skin fibroblasts. The irritant may include at least one of LPS, UV, and an oxidative stress inducer. The test group may be a group treated with the complex aroma healing serum composition or fermented hemp seed oil. The control group may be a group not treated with the sample or a group treated with non-fermented hemp seed oil. The measurement indicators may include at least one of IL-6, TNF-α, IL-1β, COX-2, and iNOS. Quantification may be performed using at least one of ELISA, qPCR, and immunoblot. Each group may be repeated three or more times. The results may be described as the mean and standard deviation. As a result of measuring the expression level of the inflammatory cytokine (IL-6) in cells treated with an inflammation-inducing substance (LPS) according to Test Example 4, the expression level in the group treated with the stimulant alone (control group) was found to be high at 1003 pg / mL, whereas in the group treated with the complex aroma healing serum composition of the present invention, it was measured at 612 pg / mL. In Test Example 5, regeneration-related indicators may be quantitatively evaluated through cell migration or extracellular matrix-related indicators. Regeneration-related indicators may include at least one of the wound area reduction rate in the scratch test, cell migration distance, and the expression level of collagen-related indicators. A scratch test can be performed by forming linear damage in a cell monolayer and then measuring the change in the area of ​​the damaged region over time under sample treatment conditions.The time points may include at least some of 0 hours, 12 hours, 24 hours, and 48 hours. Area calculation may be performed through image analysis. The wound area reduction rate may be calculated as the ratio of reduction relative to the initial area. The number of repetitions and statistical processing may be performed in the same manner as in Test Example 4. Furthermore, as a result of the scratch assay measurement according to Test Example 5, the wound area reduction rate of the control group was only 58.0%, whereas the group treated with the serum composition of the present invention showed an area reduction rate of 68.0%. In Test Example 6, the melanin production-related indicators may include at least one of tyrosinase activity, melanin content, and the expression level of genes related to melanin production. Cells may include melanin-producing cells. Melanin production stimulation may be performed by α-MSH treatment. The treatment conditions of the test group and the comparison group may be distinguished based on the presence or absence of sample treatment or the type of sample. Tyrosinase activity may be quantified by enzyme reaction-based absorbance measurement. Melanin content can be calculated through absorbance or quantitative analysis after cell lysis. The number of repetitions and statistical processing can be performed in the same manner as in Test Example 4. As a result of evaluating the activity of tyrosinase, a key enzyme for melanin production, according to Test Example 6, the enzyme activity of the group treated with the complex aroma healing serum composition of the present invention was measured to be at the level of 73% when the activity of the control group was considered as 100%. Fig. 4 is a diagram showing an example of the fatty acid composition in hemp seed oil. More specifically, Fig. 4 illustrates the major fatty acid components included in hemp seed oil and the content ratio (%) of each component in a table format. As shown in Fig. 4, hemp seed oil contains Myristic acid (29.4%), Palmitoleic acid (16.2%), Heptadecanoic acid (10.8%), Stearic acid (10.9%), Oleic acid (12.4%), and Linoleic acid (14.It contains 9%), etc., and in addition, it can be confirmed that a plurality of unsaturated fatty acids, such as linolenic acid, erucic acid, arachidonic acid, and docosahexaenoic acid, are included in small amounts. As described above, exemplary embodiments have been disclosed in the drawings and the specification. Although specific terms have been used to describe the embodiments in this specification, they are used only for the purpose of explaining the technical concept of this disclosure and are not used to limit the meaning or the scope of this disclosure as defined in the claims. Therefore, those skilled in the art will understand that various modifications and equivalent alternative embodiments are possible therefrom. Accordingly, the true technical scope of protection of this disclosure should be determined by the technical concept of the appended claims.

Claims

Claim 1 A method for preparing a complex aroma healing serum composition containing fermented oil, comprising: (a) a step of preparing a hemp seed oil raw material; (b) a step of obtaining fermented hemp seed oil by fermenting the hemp seed oil using lactic acid bacteria of the genus Lactobacillus; (c) a step of preparing a mixture of natural essential oils including at least two different types selected from lavender oil, neroli oil, sandalwood oil, orange oil, lemon oil, grapefruit oil, geranium oil, tea tree oil, chamomile oil, helichrysum oil, myrrh oil, and frankincense oil; (d) a step of introducing the fermented hemp seed oil as a base solution into a stirring tank including a stirrer, and introducing the natural essential oil mixture as a first mixture to form a second mixture; and (e) a step of uniformly mixing and aging the second mixture to complete a fermented oil-based complex aroma healing serum composition; wherein the step of forming the second mixture comprises a gauge pressure of -0.09 MPa or higher relative to atmospheric pressure inside the stirring tank.The method includes the step of driving a stirrer to stir the second mixture in a reduced pressure environment while the pressure is reduced to 0.8 MPa or less, wherein the stirring tank includes an inlet extending upward from the center of the upper surface of the tank body, wherein the inlet is cylindrical in shape, with an outer upper end open and a lower end open to the interior of the stirring tank to allow the basic solution and the first mixture to flow in, wherein the inlet includes a first filter mesh coupled to the center of the inlet and configured to filter out particulate foreign substances with a particle diameter of 100 μm or more, a pivoting part disposed below the first filter mesh and coupled to be rotatable by a hinge axis relative to the outer wall of the inlet, and a second filter mesh mounted on the upper surface of the pivoting part, configured to filter out fine particulate foreign substances with a particle diameter exceeding 10 μm and less than 100 μm, and capable of being detachably attached by magnetic force, wherein a vibration generating module is coupled to the inner lower surface of the pivoting part, and when the pivoting part is in a closed position, the vibration generating module is 50 Hz A method for manufacturing a complex aroma healing serum composition, configured to be driven with a frequency of 200 Hz and an output of 1 W to 10 W so that vibration is transmitted to the entire second filter screen through the upper surface of the rotating part, and the vibration generating module is controlled by an interlock control unit so as to be driven only when the rotating part is in a closed position. Claim 2 delete Claim 3 delete

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