Health functional food composition for height growth, bone formation, and immunity enhancement in children and adolescents

Abstract

The present invention relates to a health functional food, and more specifically, to a dual formulation (liquid and tablet) of a health functional food composition containing a combination of ingredients effective for promoting height growth, assisting bone formation, and strengthening immune function in growing children and adolescents. The health functional food composition of the present invention is designed to simultaneously promote height growth, bone formation, and immune enhancement in growing children and adolescents. The liquid formulation includes arginine and lysine, which induce growth hormone secretion, and deer antler extract, which contributes to enhancing vitality, thereby aiding in height growth. Additionally, the tablet formulation includes bone-strengthening ingredients such as calcium, vitamin D3, and vitamin K1, as well as a group of immune-boosting vitamins, to improve bone health and immune function. Consuming the two formulations in combination can increase the absorption rate in the body and maximize physiological interactions. Accordingly, the present invention demonstrates excellent efficacy as a complex health functional food capable of effectively supplying essential nutrients during the growth period.

Description

Technology Field

[0001] The present invention relates to a health functional food, and more specifically, to a health functional food composition in a dual formulation (liquid and tablet) that contains a combination of ingredients effective for promoting height growth, assisting bone formation, and strengthening immune function in growing children and adolescents. Background Technology

[0002] In modern society, interest in the health management of growing children and adolescents is increasing, and height growth, skeletal strengthening, and immune system enhancement are the factors parents prioritize most for their children's health. These bodily functions are primarily determined by growth hormone, calcium metabolism, and the activity of immune cells, making a sufficient supply of various bioactive nutrients that regulate these processes essential.

[0003] Height growth is achieved through an increase in bone length, which is regulated by the activity of the cartilage plate. To maintain and stimulate the activity of the cartilage plate, growth hormone secretion-promoting factors, particularly amino acids such as arginine and lysine, are required. Meanwhile, bone formation occurs through the action of minerals and coenzymes such as calcium, magnesium, vitamin D3, and vitamin K1, and the efficiency of these can vary significantly depending on their absorption rates and metabolic pathways within the body. Accordingly, formulations with high bioavailability and combination prescriptions are required.

[0004] Immune function is involved in resistance to infection, recovery, and the maintenance of homeostasis within the body, and this is maintained by the adequate supply of micronutrients that perform antioxidant and immune-supportive functions, such as vitamin C, vitamin E, folic acid, and zinc. During the growth period, the body's metabolism is active and nutrient consumption is high, so a deficiency of these components can easily lead to a decline in immunity.

[0005] Existing health functional foods often consisted of single-ingredient products such as vitamins or calcium supplements, and compositions capable of simultaneously realizing complex functions like height growth, bone formation, and immune enhancement in a single product were limited. Furthermore, in terms of formulation, combined liquid and tablet methods designed to balance ease of intake and absorption rates were technically complex or rarely found in the market.

[0006] Accordingly, there is a demand for complex health functional food compositions that can simultaneously satisfy height growth, skeletal strengthening, and immune enhancement functions by reflecting the physiological needs of growing children and adolescents; in particular, there is an increasing demand for the development of combination liquid and tablet compositions in which each functional ingredient is appropriately formulated according to its absorption rate and bioavailability. Prior art literature

[0007] (Patent Document 0001) KR 102572487 B(Patent Document 0002) KR 101493442 B(Patent Document 0003) KR 101252630 B(Patent Document 0004) KR 1020190091768 A The problem to be solved

[0008] The present invention was devised to solve the problems of the prior art as described above. The objective of the present invention is to provide a health functional food composition that satisfies both complex functionality and convenience of consumption by combining growth-promoting components including arginine and lysine, bone-strengthening components including calcium and vitamin D3, and immune-boosting components including vitamin C, folic acid, and zinc, and dividing and providing them in liquid and tablet formulations according to their absorption and action pathways in the body. means of solving the problem

[0009] To solve the above problems, the present invention provides a health functional food composition for height growth, bone formation, and immune enhancement in adolescents, comprising: (a) a liquid containing magnesium gluconate, zinc gluconate, sweet honey, mixed fruit concentrate, anhydrous citric acid, amino acid mixed powder, deer antler extract, enzyme-treated stevia, L-lysine hydrochloride, and L-arginine; and (b) a tablet containing cockle calcium powder, seaweed powder, vitamin C, vitamin E mixed preparation, vitamin A mixed preparation, calcium pantothenate, vitamin D3 mixed preparation, vitamin B12, vitamin B1, vitamin B2, vitamin B6, vitamin K1 mixed preparation, folic acid, red grape concentrate powder, bamboo sap extract powder, goat milk protein powder, magnesium stearate, colostrum protein powder, enzyme-treated stevia, and DL-malic acid.

[0010] It is preferable that the above liquid and tablet be packaged in a once-daily dose.

[0011] It is preferable that the L-arginine contained in the above liquid be included in a range of 0.05% by weight to 1.0% by weight.

[0012] The above liquid is Cudrania tricuspidata ( Cudrania tricuspidata It is preferable to further include a hot water extract in the range of 0.1% to 1.0% by weight.

[0013] The above tablet is snake fern ( Athyrium yokoscense It is preferable to further include a hot water extract in the range of 0.1% to 1.0% by weight. Effects of the invention

[0014] The health functional food composition of the present invention is designed to simultaneously promote height growth, bone formation, and immunity enhancement in growing children and adolescents. The liquid formulation aids in height growth by including arginine and lysine, which induce growth hormone secretion, and deer antler extract, which contributes to increased vitality. Additionally, the tablet formulation improves bone health and immune function by including bone-strengthening components such as calcium, vitamin D3, and vitamin K1, as well as a group of immune-boosting vitamins. Consuming the two formulations in combination increases the absorption rate in the body and maximizes physiological interactions. Accordingly, the present invention demonstrates excellent efficacy as a complex health functional food capable of effectively supplying essential nutrients during the growth period. Brief explanation of the drawing

[0015] Figure 1 is a photograph of the liquid and tablet forms of the health functional food composition of the present invention packaged for single use. Specific details for implementing the invention

[0016] The present invention relates to a health functional food composition designed to simultaneously satisfy three major functions—height growth, bone formation, and immune enhancement—in order to support the physical development of growing adolescents. In particular, the technical feature of the present invention is that it simultaneously improves convenience of intake and bioavailability by designing the main ingredients for each function into dual formulations (liquid and tablet) based on their physiological activity and absorption rate characteristics.

[0017] Accordingly, the present invention provides a health functional food composition for height growth, bone formation, and immune enhancement in adolescents, comprising: (a) a liquid containing magnesium gluconate, zinc gluconate, sweet honey, mixed fruit concentrate, anhydrous citric acid, amino acid mixed powder, deer antler extract, enzyme-treated stevia, L-lysine hydrochloride, and L-arginine; and (b) a tablet containing cockle calcium powder, seaweed powder, vitamin C, vitamin E mixed preparation, vitamin A mixed preparation, calcium pantothenate, vitamin D3 mixed preparation, vitamin B12, vitamin B1, vitamin B2, vitamin B6, vitamin K1 mixed preparation, folic acid, red grape concentrate powder, bamboo sap extract powder, goat milk protein powder, magnesium stearate, colostrum protein powder, enzyme-treated stevia, and DL-malic acid.

[0018] It is preferable that the above liquid and tablet be packaged in a once-daily dose.

[0019] It is preferable that the L-arginine contained in the above liquid be included in a range of 0.05% by weight to 1.0% by weight.

[0020] The above liquid composition includes L-arginine and L-lysine hydrochloride, which are amino acids that promote the secretion of growth hormone; a mixed amino acid powder involved in cell metabolism and energy production; deer antler extract that helps improve the vitality of adolescents; and enzyme-treated stevia that assists in the proliferation of beneficial intestinal bacteria and digestion and absorption. In addition, it enhances bioavailability and taste preference through minerals such as magnesium gluconate and zinc gluconate, naturally derived carbohydrate sources such as royal jelly and mixed fruit concentrate, and acidifiers such as anhydrous citric acid.

[0021] The liquid composition of the present invention may include the following as functional ingredients for growth promotion and immune enhancement:

[0022] 1. Minerals and Amino Acids: Magnesium gluconate, Zinc gluconate, L-lysine hydrochloride, L-arginine, Amino acid mixed powder (BCAA, etc.)

[0023] 2. Naturally derived carbohydrates and sugars: Agave syrup, royal jelly, fructooligosaccharide, New Origo, orange concentrate, mixed fruit concentrate

[0024] 3. Other functional ingredients: Deer antler extract, anhydrous citric acid, enzyme-treated stevia

[0025] These ingredients contribute to height growth, bone development, and immune enhancement in children and adolescents through various physiological actions, such as growth hormone induction, support for calcium absorption, proliferation of beneficial intestinal bacteria, and antioxidant functions.

[0026] As a preferred embodiment, the liquid composition may contain purified water, magnesium gluconate, zinc gluconate, orange concentrate, fructooligosaccharide, New Origo, agave syrup, sweet honey, mixed fruit concentrate, anhydrous citric acid, amino acid mixed powder, deer antler extract, enzyme-treated stevia, L-lysine hydrochloride, and L-arginine.

[0027] More specifically, the liquid composition of the present invention may include the following components within a weight percent range, based on 100 weight percent of the total. First, purified water is included in a range of 60 weight percent to 80 weight percent as the base solvent of the composition, serving as a basis for stabilizing the entire composition and dissolving the active ingredients. Magnesium gluconate, which is involved in growth and neurotransmission, is included in a range of 0.1 weight percent to 1.5 weight percent, and zinc gluconate is included in a range of 0.05 weight percent to 0.5 weight percent for immune function and growth support. Additionally, orange concentrate may be included in a range of 1 weight percent to 5 weight percent for flavor improvement and supply of naturally derived vitamins, and fructooligosaccharides and Newoligo, which act as prebiotics for gut health, are included in a range of 1 weight percent to 6 weight percent and 1 weight percent to 5 weight percent, respectively. Natural carbohydrates for sweetness and reducing glycemic load include agave syrup in an amount of 1% to 4% by weight and sugar syrup honey in an amount of 0.5% to 3% by weight, and mixed fruit concentrate for antioxidant and taste improvement is also blended in an amount of 0.5% to 3% by weight. Citric acid anhydrous is included in a range of 0.1% to 0.5% by weight to control acidity and stabilize active ingredients, and amino acid mixed powder (including BCAA) that contributes to growth hormone promotion is included in an amount of 0.1% to 1.5% by weight, L-lysine hydrochloride in an amount of 0.05% to 0.5% by weight, and L-arginine in an amount of 0.05% to 1% by weight. In addition, deer antler extract for vitality support is contained in a range of 0.05% to 0.5% by weight, and enzyme-treated stevia, which provides safety as a naturally derived sweetener, may be incorporated in a small amount of 0.01% to 0.2% by weight.

[0028] The above ingredients each perform physiologically active functions such as promoting growth, assisting in bone formation, and improving immune function, and by combining them and providing them in a liquid form, a health functional food composition effective for promoting the overall health of children and adolescents is realized.

[0029] The above liquid composition additionally contains Cudrania tricuspidata (to support immunity enhancement and antioxidant functions) Cudrania tricuspidata It is preferable to include a hot water extract of ) in a range of 0.1% to 1.0% by weight. Cudrania tricuspidata is a deciduous shrub belonging to the Moraceae family, and has traditionally been used in folk medicine for detoxification, liver protection, and inflammation relief. Recently, numerous scientific evidences regarding its physiologically active components have also been reported. The leaves and stems of Cudrania tricuspidata contain large amounts of flavonoid compounds (e.g., cudranin, rutin), beta-sitosterol, coumarin, and chlorogenic acid, which are known to have functions such as antioxidant, anti-inflammatory, immune cell activation, and improved blood circulation.

[0030] The hot water extract of Cudrania tricuspidata used in the present invention can generally be prepared by washing the leaves, stems, or whole plant of Cudrania tricuspidata, then performing a hot water extraction using purified water or distilled water at a temperature of about 80 to 100°C for 2 to 3 hours, filtering the extract, and then concentrating or powdering it. At this time, since the solid content affects the physical stability and sensory preference of the final composition, the solid content of the raw material is generally controlled to a level of 3 to 10 Brix, and if necessary, it may be processed into a purified powdered hot water extract through additional processes such as enzyme treatment, spray drying, or freeze-drying.

[0031] The hot water extract can supply beneficial bioactive substances to enhance immunity in growing children and adolescents. In particular, it can induce the maintenance of immune balance and the reduction of inflammation through mechanisms such as inducing functional activation of neutrophils, macrophages, and NK cells, improving the ability to remove reactive oxygen species (ROS) in the body, and inhibiting inflammatory cytokines such as TNF-α and IL-6. Additionally, due to its excellent antioxidant activity, it can contribute to inhibiting cell damage during the growth period and maintaining overall vitality.

[0032] Including a hot water extract of Cudrania tricuspidata in the liquid composition of the present invention is a highly effective composition for synergistically realizing growth promotion, immune balance, and antioxidant auxiliary effects.

[0033] Meanwhile, the above-mentioned tablet composition is based on cockle calcium powder and seaweed powder, which directly contribute to bone formation and maintenance, and includes a vitamin D3 mixed preparation and a vitamin K1 mixed preparation that regulate the balance of calcium metabolism in the bone. In addition, vitamins C, E, A, and B vitamins (B1, B2, B6, B12), folic acid, and calcium pantothenate are blended in a balanced manner to support immune cell function and induce antioxidant activity, and functional ingredients such as goat milk protein powder, colostrum protein powder, red grape concentrate powder, and bamboo sap extract powder are used to promote vitality and maintain immunity during the growth period. Magnesium stearate for formulation stability and DL-malic acid for acidity control are also included.

[0034] The above-mentioned tablet composition of the present invention comprises the following ingredients for the purpose of assisting in bone formation, strengthening immunity, and supplying essential vitamins to growing children and adolescents:

[0035] 1. Natural mineral and calcium sources: Cockle calcium powder, seaweed powder, DL-malic acid, magnesium stearate

[0036] 2. Vitamin Complex: Vitamin C, Vitamin E mixed preparation (DL-alpha-tocopheryl acetate), Vitamin A mixed preparation (retinyl palmitate), calcium pantothenate, Vitamin D3 mixed preparation, Vitamins B1, B2, B6, B12, Vitamin K1 mixed preparation, folic acid

[0037] 3. Functional bioactive materials: Red grape concentrate powder, bamboo sap extract powder, goat milk protein powder, colostrum protein powder

[0038] 4. Natural Sweeteners and Additives: Enzyme-treated Stevia

[0039] These ingredients improve the absorption rate of calcium and minerals, and not only aid in bone growth and maintaining bone density, but also contribute to supporting immune function and strengthening the body's antioxidant system.

[0040] As a preferred embodiment, the tablet composition may contain cockle calcium powder, seaweed powder, vitamin C, vitamin E mixed preparation, vitamin A mixed preparation, calcium pantothenate, vitamin D3 mixed preparation, vitamin B12, vitamin B1, vitamin B2, vitamin B6, vitamin K1 mixed preparation, folic acid, red grape concentrate powder, bamboo sap extract powder, goat milk protein powder, magnesium stearate, colostrum protein powder, enzyme-treated stevia, and DL-malic acid.

[0041] More specifically, the tablet composition of the present invention may include the following components within a weight percent range, based on 100 weight percent of the total composition. Cockle calcium powder, a major source of minerals, is included in a range of 10% to 25% by weight to provide calcium, which is important for bone formation and maintaining bone density, as a naturally derived raw material; seaweed powder is included in a range of 1% to 5% by weight to supplement minerals and improve bioavailability. Vitamin C, which contributes to antioxidant and immune enhancement, is included in a range of 1% to 5% by weight; a vitamin E mixed preparation may be included in a range of 0.2% to 1% by weight considering antioxidant stability and absorption; and a vitamin A mixed preparation may be included in a range of 0.05% to 0.5% by weight. Calcium pantothenate, which is involved in skin and mucous membrane health and metabolic function, is included in a range of 0.1% to 1% by weight; and a vitamin D3 mixed preparation is included in a small amount of 0.01% to 0.2% by weight to promote calcium absorption. Vitamins B1, B2, and B6, which are involved in energy metabolism and nerve function, are each included in an amount of 0.05% to 0.5% by weight, and Vitamin B12 is included as a highly active trace component in an amount of 0.001% to 0.01% by weight. A Vitamin K1 mixed preparation is included in an amount of 0.01% to 0.2% by weight as a component that induces calcium settlement in bone tissue, and folic acid is included in an amount of 0.01% to 0.2% by weight to assist in cell division and blood production. As functional auxiliary ingredients, red grape concentrate powder and bamboo sap extract powder, which are expected to have antioxidant and anti-inflammatory effects, are each included in an amount of 0.5% to 3% by weight, and goat milk protein powder and colostrum protein powder, which can contribute to immune support and growth promotion, are each included in an amount of 1% to 5% by weight. Magnesium stearate is included in an amount of 0.1% to 1% by weight to improve the physical stability and fluidity of the tablet, and DL-malic acid is included in an amount of 0.1% to 1% by weight to control taste and acidity. In addition, enzyme-treated stevia, a natural sweetener for improving sweetness, is incorporated in a small amount in the range of 0.01% to 0.1% by weight.

[0042] By scientifically combining various functional ingredients in this manner and providing them in a tablet formulation, the present invention can realize a health functional food composition capable of efficiently promoting bone formation, immunity enhancement, and essential vitamin supplementation for growing adolescents.

[0043] The above-mentioned tablet composition additionally contains snake fern ( Athyrium yokoscense It is preferable to further include a hot water extract of ) in the range of 0.1% to 1.0% by weight. Pteridium aquilinum is an alpine fern with thick leaves and perennial growth characteristics, and is a native plant that has traditionally been used for detoxification, antibacterial, and bone strengthening purposes. According to various recent studies, pteridium aquilinum contains physiologically active substances such as flavonoids, saponins, organic acids, tannins, and phenolic compounds, and is attracting attention as a functional plant that can contribute to antioxidant, anti-inflammatory, antibacterial, immunomodulatory, and calcium metabolism supportive effects.

[0044] The above-mentioned hot water extract is generally prepared by harvesting, washing, and drying the above-ground parts (including leaves and stems) of the snake fern, then performing hot water extraction using purified water as a solvent at a temperature of 80°C to 100°C for 2 to 4 hours, filtering and concentrating the resulting extract, and then producing it in the form of an extract powder with a solid content of approximately 5 to 10% through a spray drying or freeze-drying process. If necessary, dietary fiber-derived excipients such as food-grade dextrin, maltodextrin, or gum arabic may be used together to improve the flowability of the powder and the compression stability of the tablet.

[0045] This hot water extract of the snake fern can have a positive effect on skeletal development and the maintenance of immune balance in growing adolescents through mechanisms of action, such as regulating the expression of calcium-binding proteins related to bone health, protecting osteocytes from oxidative stress, scavenging reactive oxygen species (ROS), and activating immune cells and regulating cytokine expression. Furthermore, since a synergistic effect is expected when used in combination with bone metabolism-related components such as vitamin D3, calcium, and vitamin K1, it is highly suitable for use as a core functional material in the purified composition of the present invention.

[0046] Therefore, including a hot water extract of the snake fern in the purified composition of the present invention contributes to realizing a differentiated functional composition capable of comprehensively supporting skeletal strengthening and immune health of growing adolescents.

[0047] The health functional food composition of the present invention can perform the following manufacturing process for packaging liquids and tablets for single use.

[0048] First, the liquid composition and the tablet composition are prepared separately. The liquid composition is uniformly stirred in a mixing tank containing active ingredients, sweeteners, acidity regulators, preservatives, etc., and then its physical properties are stabilized through heating or cooling processes if necessary, and impurities and bubbles are removed through filtering and vacuum degassing processes. The tablet composition is prepared by weighing and mixing each functional ingredient and excipient, followed by granulation and drying, and then compressing it into tablets using an automatic tablet press. If necessary, it is coated to ensure stability.

[0049] To provide liquid and tablets in separate compartments for single-use, a dedicated container with a double-cap structure is used. This container consists of a bottle at the bottom for holding the liquid and a transparent cap at the top for storing tablets, and the top cap is designed to allow for separate sealing.

[0050] In the manufacturing process, a liquid composition is first filled in a precise amount into the bottom bottle, and the bottle opening is temporarily sealed or a cap is lightly placed over it to prevent leakage or contamination of the liquid. Subsequently, one or two tablets are inserted into a separately prepared top transparent cap using an automatic tablet transfer device, and the top is sealed by heat sealing with aluminum foil or composite film. Then, the bottle filled with the liquid and the top cap with the tablets inserted are mechanically assembled to complete the final product.

[0051] After inspecting the finished product for sealing, liquid fill volume, number of tablets, and appearance defects, it is individually packaged and shipped. The end consumer presses the top cap to drop the tablet into the liquid, shakes to mix, and then consumes it.

[0052] This double-cap packaging method prevents interactions between ingredients or a decrease in stability by storing the tablet and liquid separately, and also contributes to improving absorption and freshness by mixing them immediately before consumption. Therefore, it is very useful as a single-use composite packaging form for health functional foods or functional beverages.

[0053] As such, the present invention scientifically combines each component to organically link and provide growth promotion, bone formation, and immune enhancement necessary for growing adolescents, and by implementing this in a combined liquid and tablet formulation, it offers superior effects that differentiate it from existing single-function health functional foods in terms of complex functionality, absorption rate, and ease of consumption.

[0054] The present invention will be described in detail below through specific embodiments. The following embodiments describe a preferred embodiment of the present invention, and the scope of the present invention is not limited by the matters described above.

[0055] [Example]

[0056] Example 1

[0057] The liquid composition comprises magnesium gluconate (0.5 wt%), zinc gluconate (0.2 wt%), sweet honey (2.0 wt%), mixed fruit concentrate (1.5 wt%), anhydrous citric acid (0.3 wt%), amino acid mixed powder (0.8 wt%), deer antler extract (0.3 wt%), enzyme-treated stevia (0.05 wt%), L-lysine hydrochloride (0.3 wt%), and L-arginine (0.5 wt%), and the remainder is purified water.

[0058] The tablet composition consisted of cockle calcium powder (15 wt%), seaweed powder (3 wt%), vitamin C (2 wt%), vitamin E mixed preparation (0.5 wt%), vitamin A mixed preparation (0.2 wt%), calcium pantothenate (0.5 wt%), vitamin D3 mixed preparation (0.1 wt%), vitamin B group and K1 mixed preparation (total 1.2 wt%), folic acid (0.2 wt%), red grape concentrate powder (2 wt%), bamboo sap extract powder (1 wt%), goat milk protein powder (3 wt%), colostrum protein powder (2 wt%), DL-malic acid (0.5 wt%), enzyme-treated stevia (0.05 wt%), and magnesium stearate (0.5 wt%). The tablets were compressed in a press, film-coated, and packaged.

[0059] Example 2

[0060] Composed in the same manner as Example 1, but with Cudrania tricuspidata (in the liquid composition) Cudrania tricuspidata 0.3% by weight of the hot water extract of ) was added. The extract was prepared by extracting the leaves and stems of Cudrania tricuspidata with purified water at 90°C for 3 hours, followed by filtration, concentration, and spray drying to powder.

[0061] Example 3

[0062] In addition to the composition of Example 2, the tablet composition contains snake fern ( Athyrium yokoscense 0.3% by weight of a hot water extract of ) was added. The extract was prepared as a powder by hot water extracting the above-ground parts of the snake fern with purified water at 90°C for 3 hours, filtering and concentrating, and then spray-drying.

[0063] Comparative example

[0064] A commercially available health functional food for adolescents, A (multivitamin tablets, containing calcium), was used as the comparison subject.

[0065] Experimental Example

[0066] Experimental Example 1: Comparison of Height Growth and Bone Formation Indicators Using Experimental Animals

[0067] 1. Purpose

[0068] This experiment is intended to confirm the effects of the health functional food compositions of Examples 1 to 3 and the composition of the comparative example on height growth and bone formation during adolescence.

[0069] 2. Method

[0070] Animals used: 40 male white Sprague-Dawley rats, 4 weeks old

[0071] Group composition (n=10):

[0072] Group A: Administration of Example 1

[0073] Group B: Administration of Example 2 (including Cudrania tricuspidata)

[0074] Group C: Example 3 administration (including Cudrania tricuspidata + Pteridium aquilinum)

[0075] Group D: Comparative Example (Administration of commercially available health functional food)

[0076] Method of administration: Administer each composition orally once a day for 6 weeks continuously.

[0077] Measurement items:

[0078] Weekly measurement of body length (length from nose to base of tail).

[0079] Femoral length, bone density (BMD, DEXA)

[0080] Measurement of serum IGF-1, ALP (alkaline phosphatase), and osteocalcin levels

[0081] 3. Result Summary

[0082] [Table 1]

[0083]

[0084] Group C showed the highest femoral length and BMD, along with significantly increased levels of IGF-1 and osteocalcin (p<0.05). Additionally, Group B demonstrated superiority in bone growth indicators compared to Groups A and D. Comparative example Group D showed no significant difference in bone density other than an increase in ALP levels.

[0085] Experimental Example 2: Comparison of Immune Function Activation Effects

[0086] 1. Purpose

[0087] This experiment is intended to compare the effects of each composition on immune cell activity and the regulation of inflammatory cytokines.

[0088] 2. Method

[0089] After administering to the same group for 4 weeks, blood was collected from peripheral blood.

[0090] Measurement items:

[0091] White blood cell count (WBC), lymphocyte ratio

[0092] Measure NK cell activity by flow cytometry

[0093] Serum levels of TNF-α, IL-6, and IL-10 (using ELISA kit)

[0094] 3. Result Summary

[0095] [Table 2]

[0096]

[0097] In Group C, NK cell activity and IL-10 levels were the highest, while TNF-α and IL-6 were significantly lower. Group B showed a similar trend, confirming the immunoadjuvant effect of administering Cudrania tricuspidata alone. In Comparative Example Group D, there was almost no change in NK cell activity.

[0098] Experimental Example 3: Evaluation of Antioxidant Activity (in vivo and in vitro)

[0099] 1. Purpose

[0100] The antioxidant function of each composition is compared and evaluated in terms of ROS removal and antioxidant enzyme activity.

[0101] 2. Method

[0102] Analysis of liver tissue and serum samples after animal experiments

[0103] Antioxidant-related measurement items:

[0104] SOD (superoxide dismutase) activity in serum and liver tissue

[0105] MDA (malondialdehyde) concentration

[0106] DPPH radical scavenging activity (in vitro experiments conducted concurrently)

[0107] 3. Result Summary

[0108] [Table 3]

[0109]

[0110] Group C showed the highest SOD activity and the lowest MDA levels. From this, it was found that the inhibitory effect on oxidative stress was excellent. Group B also showed a significant improvement in antioxidant indicators compared to Example 1. In the DPPH experiment, the extract from Group C also showed a scavenging ability of over 85%.

[0111] Synthesizing the above results, it can be confirmed that Example 3 exhibits the best results in growth promotion, bone health, immune regulation, and antioxidant function. In particular, Cudrania tricuspidata is effective in terms of immunity and antioxidants, while Pteris multifida enhanced the synergistic effects of bone metabolism and antioxidants. The composition of the present invention has been experimentally proven to be superior to existing commercially available products in terms of complex functionality.

Claims

Claim 1 A health functional food composition for height growth, bone formation, and immune enhancement in adolescents comprising the following: (a) magnesium gluconate, zinc gluconate, royal jelly, mixed fruit concentrate, anhydrous citric acid, amino acid mixed powder, deer antler extract, enzyme-treated stevia, L-lysine hydrochloride, L-arginine, and Cudrania tricuspidata ( Cudrania tricuspidata (b) a liquid comprising a hot water extract powder of ) and (b) cockle calcium powder, seaweed powder, vitamin C, vitamin E mixed preparation, vitamin A mixed preparation, calcium pantothenate, vitamin D3 mixed preparation, vitamin B12, vitamin B1, vitamin B2, vitamin B6, vitamin K1 mixed preparation, folic acid, red grape concentrate powder, bamboo sap extract powder, goat milk protein powder, magnesium stearate, colostrum protein powder, enzyme-treated stevia, DL-malic acid and snake fern ( Athyrium yokoscense Tablets containing hot water extract powder of ).

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