Tablet-type soil remediation agent containing microorganisms and a manufacturing method thereof

Abstract

The present invention relates to a purified soil remediation agent containing functional complex yeast microorganisms and a method for manufacturing the same. More specifically, it relates to a microbial formulation that preserves the vitality of microorganisms through a low-temperature process and enhances the settlement efficiency within the soil through a porous carrier and an organic energy source. The purified soil remediation agent according to the present invention comprises a functional complex yeast microbial group having nitrogen fixation, phosphate decomposition, and potassium decomposition functions; a carbon source organic material including plant meal, bone meal, and fish meal; and a mineral raw material having a porous structure; and is characterized by being composed in the form of pellets of a certain size. The manufacturing method of the present invention prevents premature metabolic consumption of microorganisms by precisely controlling the mixing temperature, and induces microorganisms into a stable dormant state by adjusting the moisture content of the product to an optimal range through low-temperature air drying after molding. The soil remediation agent manufactured with this composition maintains the number of effective viable bacteria within the product above a certain level and promotes the solubilization of insoluble nutrients when applied to the soil, thereby improving the chemical properties of the soil and providing the effect of enhancing overall growth indicators, including crop sugar content and enlargement.

Description

Technology Field

[0001] The present invention relates to a microorganism-containing soil conditioner for improving soil in which nutrients have accumulated excessively due to excessive fertilization and continuous cultivation of crops.

[0002] Specifically, the invention relates to a composition in the form of a tablet pellet containing a functional complex yeast microorganism as an active ingredient, and comprising a carbon source organic material including plant meal, bone meal, and fish meal, and a porous mineral raw material blended in an optimal ratio.

[0003] In addition, the invention relates to a purified soil remediation agent containing microorganisms capable of restoring soil self-purification capacity and enhancing crop productivity by precisely controlling mixing and drying temperatures to maintain microbial vitality and promoting nitrogen fixation, phosphate decomposition, and potassium decomposition within the soil, and a method for manufacturing the same. Background Technology

[0004] With the recent rise in interest in sustainable agriculture, research into soil amendment technologies utilizing microorganisms is being actively conducted to address soil pollution and reduced self-purification capacity caused by the overuse of chemical fertilizers. In particular, the use of beneficial microorganisms, which decompose excessively accumulated nutrients in the soil and alleviate salinity stress to enhance crop productivity, is establishing itself as a key element of eco-friendly agriculture.

[0005] In the past, various microbial formulation technologies have been proposed to solve these problems. Specifically, Registered Patent No. 10-1481766 proposed a technology for reducing soil salinity concentration by immobilizing a microbial formulation containing a carbon source, a nitrogen source, an organic acid, and a microbial culture solution onto a carrier composed of calcium silicate, rice bran, and distiller's lees. Additionally, Published Patent No. 10-2015-0003594 proposed a process for producing powder and liquid active solution by mixing a mixed soil microbial strain with raw materials such as rice bran, peat husk, and peat moss, heating and inoculating the mixture at 90°C, and then culturing it.

[0006] However, the aforementioned conventional technologies present various difficulties in terms of industrial practical application and field application. First, while methods involving adsorbing microorganisms onto a carrier or manufacturing them in powder form extend the shelf life compared to liquid formulations, they present problems such as dust generation during soil application or inconsistent colonization rates of microorganisms depending on the environment. Additionally, methods involving inoculating microorganisms at high temperatures above 90°C or undergoing long-term culture and maturation processes involve complex manufacturing processes and impose thermal stress on the microorganisms, which limits the ability to ensure quality uniformity and maintain a consistent viable cell count in the final product.

[0007] Furthermore, existing microbial formulations are primarily focused on comprehensive environmental improvement, such as soil purification or salt removal, and thus suffer from a limitation in that they lack the functional expertise to directly circulate and solubilize nutrients essential for plant growth, such as nitrogen, phosphorus, and potassium, within the soil. In particular, organic carriers like rice bran have the disadvantage of being decomposed too rapidly by microorganisms in the soil, preventing the microbial vitality from being sustained for an extended period. Accordingly, there is an urgent need for an invention regarding a purified microbial soil remediation agent technology that establishes a low-temperature treatment process to minimize microbial mortality during manufacturing and consists of bone meal, fish meal, and porous mineral raw materials in an optimized blending ratio to help microorganisms survive and colonize the soil for a long time. Prior art literature

[0008] (1) Korean Registered Patent No. 10-1481766 (2015.01.12)(2) Korean Published Patent No. 10-2015-0003594 (2015.01.09) The problem to be solved

[0009] The problem that the present invention aims to solve is to overcome the microbial inactivation issues inherent in existing microbial preparations during the manufacturing process, as well as the low persistence and functional limitations during field application. Specifically, the present invention aims to solve the following detailed problems.

[0010] First, to solve the problem where the viable cell count decreased due to excessive thermal stress on microorganisms during the high-temperature inoculation (90℃) or hot-air drying (60℃) processes of existing technology, we aim to establish a low-temperature manufacturing process capable of maintaining a constant viable cell count in the final product by precisely controlling the mixing and drying temperatures.

[0011] Second, we aim to provide a high-performance soil remediation agent that can significantly reduce the use of chemical fertilizers while maximizing crop productivity by combining functional complex yeast strains specialized in nitrogen fixation, phosphate solubilization, and potassium decomposition in the soil in optimal proportions, going beyond the simple function of removing salts.

[0012] Third, to compensate for the disadvantage that organic carriers such as rice bran decompose too rapidly in the soil, causing microbial vitality to disappear in a short period, we aim to secure sustained release of the fertilizer effect by mixing bone meal, fish meal, and porous mineral raw materials to simultaneously provide a colonization environment for microorganisms and a continuous nutrient supply.

[0013] Fourth, we aim to provide a microbial preparation in the form of tablet pellets with a certain strength and moisture content to improve the handling inconveniences of liquid or powder microbial preparations, such as short shelf life and dust generation during application, and to facilitate mechanized fertilization. means of solving the problem

[0014] To solve the above-mentioned problem, the present invention provides a purified soil remediation agent containing microorganisms comprising 0.5 to 2.0 weight% of a functional complex yeast microbial group, 60.0 to 62.0 weight% of a carbon source organic material including at least one of vegetable meal, bone meal, and fish meal, and 36.0 to 39.5 weight% of a mineral raw material.

[0015] The soil remediation agent according to the present invention is characterized by the above components being uniformly mixed and then formed into a tablet-type pellet. Effects of the invention

[0016] The purified soil remediation agent containing microorganisms according to the present invention and the method for manufacturing the same have the following effects.

[0017] First, unlike existing microbial preparations which struggled to maintain viable cell counts due to high-temperature processes, the present invention adopts a low-temperature mixing process of 30°C or lower and a low-temperature air drying process of 35°C or 45°C, thereby maintaining the viable cell count in the product at 1.0*10^6 CFU / g or higher and improving storage stability.

[0018] Second, to solve the problem of rapid decomposition that occurs when using rice bran (vegetable meal) alone, bone meal, fish meal, and porous mineral raw materials are mixed in optimal proportions. This simultaneously provides microorganisms with a porous shelter protected from external environmental changes and an energy source that aids in initial settlement and proliferation, thereby increasing the survival rate of microorganisms in the soil and the sustainability of the fertilizer effect.

[0019] Third, by arranging functional complex yeast strains specialized in nitrogen fixation, phosphate solubilization, and potassium decomposition according to function, it provides a soil restoration effect that goes beyond simple soil environment improvement to promote nutrient cycling within the soil and enhance crop productivity.

[0020] Fourth, it is composed of refined pellets, so there is no dust generated during application and mechanized fertilization is easy. It also creates an environment where microorganisms can rapidly take root after being applied to the soil, thereby ensuring both work efficiency and economic viability in actual fields. Brief explanation of the drawing

[0021] FIG. 1 is a flowchart showing the overall manufacturing process of a purified soil remediation agent containing microorganisms according to one embodiment of the present invention in steps. Figure 2 is a graph showing a mutual comparison of the performance of maintaining the number of viable microorganisms according to changes in drying temperature during the manufacturing process between the present invention and the prior art. Figure 3 is a field photograph showing the late-stage growth condition of cherry tomatoes and the fruit setting quality compared with the control group after applying the soil restoration agent of the present invention. Figure 4 is a table showing the results of the analysis of the initial chemical characteristics of the test soil (loam) before conducting an experiment to verify the effects of the present invention. Figure 5 is a table showing the results of the analysis of the chemical characteristics and nutrient components of the soil that changed for each test plot after treatment with the soil remediation agent of the present invention. Figure 6 is a table showing the results of major growth and quality investigations, such as sugar content, weight per fruit, and chlorophyll levels, of cherry tomatoes harvested by treating with the present invention. Specific details for implementing the invention

[0022] It should be noted that in the following description, only the parts necessary for understanding the embodiments of the present invention are explained, and the description of other parts will be omitted to the extent that it does not deviate from the gist of the present invention.

[0023] The terms and words used in the specification and claims described below should not be interpreted as being limited to their ordinary or dictionary meanings, but should be interpreted in a meaning and concept consistent with the technical spirit of the invention, based on the principle that the inventor can appropriately define the concept of the terms to best describe his invention. Accordingly, the embodiments described in this specification and the configurations illustrated in the drawings are merely preferred embodiments of the invention and do not represent all aspects of the technical spirit of the invention; therefore, it should be understood that various equivalents and modifications capable of replacing them may exist at the time of filing this application.

[0024] FIG. 1 is a process flow diagram showing a method for manufacturing a purified soil remediation agent containing microorganisms according to the present invention. FIG. 2 is a graph showing the correlation between the survival rate of microorganisms and the drying temperature according to the numerical limitations of the present invention. The manufacturing method of the present invention according to FIG. 1 and FIG. 2 comprises the steps of: sorting carbon source organic matter and mineral raw materials using a magnet and a sorter and introducing them into a raw material storage unit (S10); introducing a functional complex yeast microbial group into the raw material storage unit and mixing it with carbon source organic matter and mineral raw materials to adsorb it onto the surface of the organic matter and mineral raw materials (S20); introducing the mixed raw materials into a granular molding machine to form them into pellets (S30); blow-drying the molded pellets at a temperature of 35 to 45°C (S40); and sorting and packaging the dried pellets (S50).

[0025] In the step (S10) of sorting carbon source organic materials and mineral raw materials using a magnet and a sorter and feeding them into a raw material storage unit, a carrier serving as the physical basis of the present invention is prepared. In this step, by removing metallic foreign substances and large particles contained in the raw materials, damage to the pellet molding machine in the subsequent step (S30) of feeding the mixed raw materials into a granular molding machine to form pellets can be prevented, and the uniformity of product quality can be ensured.

[0026] The mineral raw material comprises at least one selected from the group consisting of perlite, bentonite, maifan stone, diatomite, illite, zeolite, and vermiculite. The mineral raw material is characterized by having a porous structure, which is intended to provide a physical shelter for microorganisms to be protected from rapid external environmental changes, including temperature, humidity, and pH. In particular, zeolite and vermiculite possess high cation exchange capacity (CEC) and perform a sustained-release function by adsorbing nutrients in the soil and releasing them slowly.

[0027] In the step (S10) of sorting carbon source organic matter and mineral raw materials using a magnet and a sorter and feeding them into a raw material storage unit, the carbon source organic matter fed consists of a mixture of 55 to 65 weight% of plant meal, 20 to 25 weight% of bovine bone meal, and 15 to 20 weight% of fish meal. This specific content ratio is based on a nutritional balance to improve the initial survival of microorganisms and the soil settlement rate.

[0028] In this case, the plant meal is most preferably rice bran according to one embodiment of the present invention, but is not limited thereto. The plant meal is a concept that encompasses by-products remaining after extracting active ingredients from plant raw materials. In addition to the rice bran mentioned above, at least one selected from the group consisting of soybean meal, rapeseed meal, cottonseed meal, perilla meal, sesame meal, and distillers' grains may be used alone or in combination. These plant meals commonly contain abundant amino acids and organic nutrients necessary for the cultivation of yeast microorganisms. In particular, when rice bran is used as the main component and other plant meals are mixed in, a complex metabolic product is formed that can contribute to improving the chlorophyll (SPAD) content and sugar content of crops by providing more diverse nutrients to microorganisms than when using a single raw material.

[0029] Bovine bone meal complements non-biodegradable plant meal to provide microorganisms with a continuous source of phosphate and minerals. If less than 20% by weight is added, the effect of increasing crop sugar content and fruit enlargement may be negligible, and if more than 25% by weight is added, there is a risk of dust generation due to reduced inter-particle bonding strength during pellet molding.

[0030] Fish meal helps activate yeast by supplying a high concentration of natural protein. If it is less than 15% by weight, the metabolic activity of microorganisms does not increase sufficiently, and if it exceeds 20% by weight, there is a risk of gas damage due to excess nitrogen, so it is desirable to limit it to the above range.

[0031] In the step (S20) of introducing a functional complex yeast microbial group into a raw material storage unit and mixing it with carbon source organic matter and mineral raw materials to adsorb it onto the surface of the organic matter and mineral raw materials, the functional complex yeast microbial group introduced consists of a nitrogen-fixing yeast group, a phosphate-degrading yeast group, and a potassium-degrading yeast group. By preparing a complex formulation for each of the aforementioned groups, the supply efficiency of NPK, which is one of the three key elements of crop growth, can be increased.

[0032] First, nitrogen-fixing yeasts that fix atmospheric nitrogen and convert it into a form available to plants include at least one of Lipomyces starkeyi, Rhodotorula mucilaginosa, Candida tropicalis, Debaryomyces hansenii, and Metschnikowia reukaufii. These promote nitrogen cycling in the soil, thereby reducing the use of chemical nitrogen fertilizers while assisting in crop protein synthesis and strengthening plant vigor.

[0033] Second, the phosphate-degrading yeast group that solubilizes insoluble phosphorus in the soil to increase plant absorption capacity includes at least one of Saccharomyces cerevisiae, Pichia jadinii, Hanseniaspora uvarum, Cryptococcus laurentii, and Meyerozyma caribbica. In particular, these strains significantly increase the available phosphorus content and directly contribute to the improvement of fruit sugar content and quality by breaking the bonds of hardened phosphate through the secretion of organic acids in the soil.

[0034] Third, the potassium-degrading yeast group that releases potassium trapped within the mineral structure includes at least one of Pichia guilliermondii, Debaryomyces hansenii, Kluyveromyces marxianus, Torulaspora delbrueckii, and Cryptococcus albidus. These increase the availability of potassium in the soil, thereby improving the crop's moisture regulation ability and maintaining strong vitality until the late stages of growth, which promotes an increase in final yield. Additionally, the step (S20) of introducing and mixing the functional complex yeast microbial group into the raw material storage unit is preferably performed for 5 to 15 minutes while maintaining the internal temperature of the mixer at 30°C or lower. If the mixing time is less than 5 minutes, there is a risk that the functional complex yeast microbial group may not be sufficiently and uniformly adsorbed onto the surface of the carbon source organic matter and mineral raw materials, resulting in an uneven distribution of viable cell counts inside the pellet; if the mixing time exceeds 15 minutes, there is a risk that the vitality of the yeast microbial group may decrease due to frictional heat and physical stress caused by excessive stirring. Therefore, by mixing for 5 to 15 minutes while maintaining the internal temperature of the mixer at 30℃ or lower, the functional complex yeast microbial group can be uniformly adsorbed onto the surface of the carbon source organic material and mineral raw material, and subsequently stably immobilized inside the purified pellet.

[0035] In the step (S30) of feeding the mixed raw materials into a granular molding machine to form them into pellets, tablet-shaped pellets with a diameter of 5 to 7 mm are formed using an extrusion method. By forming pellets using an extrusion method, the molding pressure can be precisely controlled compared to a general electric assembly method using a rotating disc, etc. While the electric assembly method utilizes friction and collision between particles, which can cause random physical damage to the cell walls of microorganisms, the extrusion method compresses the raw materials at a constant pressure to form them, thereby allowing the functional yeast microbial community inside to be uniformly settled within the pellet without being crushed.

[0036] By setting the diameter of the refined pellets to 5 to 7 mm, physical stability is provided during fertilization using agricultural machinery such as fertilizer spreaders, while simultaneously optimizing the contact area with the soil surface to prevent excessive leakage or delayed release of nutrients. In particular, the high-density compressed pellet form suppresses dust generation caused by breakage during distribution, thereby providing both convenience of fertilization and a pleasant working environment. Furthermore, a structure can be formed in which functional microorganisms and carbon-source organic matter are immobilized within the particles.

[0037] In the step (S40) of inducing a dormant state of microorganisms by blow-drying the molded pellets, the molded pellets are blow-dried at a drying temperature of 35 to 45°C to control the weight moisture content of the final product to a range of 8 to 12 weight%.

[0038] Figure 2 quantitatively illustrates the effects of drying temperature and product moisture content on the physiological state of microorganisms during the manufacturing process of the present invention. First, referring to the microbial survival rate curve according to temperature, which is the graph on the left side of Figure 2, it can be seen that an "optimal safe zone" is formed where the survival rate of microorganisms is maximized to over 95% in the drying temperature range of 35°C to 45°C. Beyond this zone, when the temperature begins to reach 50°C, denaturation of yeast cell proteins begins, causing the survival rate to start to decline. Furthermore, in the temperature range of 60°C or higher, which is mainly used in the prior art, the number of viable cells drops sharply to 10^4 CFU / g or less, effectively losing the soil remediation function. Therefore, the low-temperature drying of 45°C or lower specified by the present invention corresponds to an essential technical limit for maintaining the vitality of microorganisms.

[0039] Next, looking at the correlation between moisture content, metabolic activity, and preservation stability through the graph on the right in Figure 2, it can be confirmed that when the weight moisture content is controlled to 12% by weight or less, the metabolic activity of microorganisms decreases sharply and they enter a stable dormant state. This causes microorganisms to stop unnecessary energy consumption and maintain a spore state or a stationary state capable of withstanding the external environment, thereby significantly extending the shelf life of the product. In particular, the preservation stability curve reaches a maximum value of over 95% when the moisture content is in the range of 8 to 12% by weight; however, if moisture is excessively removed to less than 8% by weight, even the bound water inside the microorganisms evaporates, causing structural collapse of the cell membrane and resulting in a decrease in preservation stability.

[0040] Accordingly, by performing air drying on the molded pellets at a drying temperature of 35 to 45°C to control the weight moisture content of the final product to within the range of 8 to 12% by weight, the metabolic activity of microorganisms is rapidly reduced, allowing them to enter a stable dormant state and secure resistance to changes in the external environment, thereby minimizing the loss of viable bacteria during the storage and distribution process of the product.

[0041] In the step of sorting and packaging dried pellets (S50), the physical specifications and moisture content of the dried products are finally inspected. That is, the uniformity of product quality is ensured by sorting and removing defective products that deviate from the diameter of 5 to 7 mm set in the molding step (S30) or are damaged during the drying process.

[0042] [Experimental Example 1]

[0043] Figure 3 is a field photograph showing the late-stage growth condition of cherry tomatoes and fruit set quality compared with a control group after application of the soil remediation agent of the present invention. Figure 4 is a table showing the results of the analysis of the initial chemical characteristics of the test soil (loam) before conducting the experiment to verify the effect of the present invention. Figure 5 is a table showing the results of the analysis of the chemical characteristics and nutritional components of the soil changed by each test group after treatment with the soil remediation agent of the present invention. Figure 6 is a table showing the results of major growth and quality investigations, such as sugar content, weight per fruit, and chlorophyll levels, of cherry tomatoes harvested after treatment with the present invention.

[0044] To verify the effectiveness of the soil remediation agent of the present invention, a field test was conducted on cherry tomatoes at a leased field in Chuncheon-si, Gangwon-do from August 1, 2025, to November 14, 2025. The test plots were arranged in a randomized block design with three replications, and the treatment plots were set as a control group, a standard amount treatment group of the composition of the present invention (50 kg / 10a soil mixing), a double amount treatment group (100 kg / 10a soil mixing), and a commercially available Bacillus subtilis microbial agent treatment group (15 kg / 10a). In the field photographs taken during the late growth stage after planting (November 14, 2025) (corresponding to Fig. 3), it was confirmed that the treatment groups of the composition of the present invention (standard and double amounts) showed better growth and stable fruit setting and development compared to the control group.

[0045] The composition of the present invention used in this packaging test was formulated based on the production of 50 kg of product. Specifically, 0.5 kg of a functional complex yeast microbial group was added, and 18.0 kg of rice bran, 6.0 kg of bovine bone meal, and 6.0 kg of fish meal were mixed as organic carbon sources. In addition, 14.5 kg of zeolite having a porous structure and 5.0 kg of bentonite, a clay mineral, were mixed as mineral raw materials to prepare a total composition of 50 kg.

[0046] Meanwhile, as a result of analyzing the chemical characteristics of the test soil (loam) before treatment (corresponding to Figure 4), the pH ranged from 6.20 to 6.35 and the EC ranged from 1.35 to 1.50 dS / m, indicating that salt accumulation was not severe. Organic matter (OM) was approximately 25.3 to 25.8 g / kg, available phosphorus (P₂O₅) was 456 to 486 mg / kg, and exchangeable cations (K, Ca, Mg) and CEC were found to be K 0.80 to 0.85 cmol(+) / kg, Ca 5.81 to 5.96 cmol(+) / kg, Mg 1.60 to 1.87 cmol(+) / kg, and CEC 11.50 to 11.70 cmol(+) / kg, respectively. Subsequently, as a result of analyzing the chemical characteristics of the soil after harvest (corresponding to Fig. 5), it was confirmed that the EC, OM, available phosphorus (P₂O₅), exchangeable potassium (K), magnesium (Mg), and CEC tended to increase in the treatment group of the composition of the present invention compared to the control group. For example, compared to the control group, improvements were confirmed in the standard and double amount treatment groups, with EC (1.25→1.55~1.60 dS / m), OM (20.2→24.5~25.1 g / kg), P₂O₅ (365→445~456 mg / kg), K (0.51→0.68~0.71 cmol(+) / kg), Mg (1.22→1.55~1.58 cmol(+) / kg), and CEC (10.40→11.55~11.56 cmol(+) / kg).

[0047] In addition, as a result of investigating the growth and quality of cherry tomatoes after harvest (corresponding to Fig. 6), the sugar content increased from 6.2 Brix in the control group to 6.6 Brix in the standard treatment group and 6.8 Brix in the double treatment group, the diameter per fruit increased from 2.45 cm in the control group to 2.55 cm in the standard treatment group and 2.57 cm in the double treatment group, and the weight per fruit showed an increasing trend from 14.55 g in the control group to 15.10 g in the standard treatment group and 15.15 g in the double treatment group. Chlorophyll (SPAD) also increased from 42.8 in the control group to 44.3 in the double treatment group, and the comparison (0~5) evaluation was found to be 0 in the treatment group of the composition of the present invention, so no fertilizer damage was observed. Accordingly, according to the present experimental example, it was confirmed that the microbial-containing purified soil remediation agent of the present invention contributes to the improvement of soil chemical properties (especially EC, OM, P₂O₅, K, Mg, CEC) and the improvement of crop sugar content, fruit size, fruit weight, and chlorophyll indicators in cherry tomato cultivation fields without causing damage. Explanation of the symbols delete

Claims

Claim 1 0.5 to 2.0 wt% of a functional complex yeast microbial group; 60.0 to 62.0 wt% of a carbon source organic material including vegetable meal, bone meal, and fish meal; and 36.0 to 39.5 wt% of a mineral raw material; wherein the functional complex yeast microbial group comprises: a nitrogen-fixing yeast group comprising at least one of Lipomyces starkeyi, Rhodotorula mucilaginosa, Candida tropicalis, Debaryomyces hansenii, and Metschnikowia reukaufii; and a phosphate-degrading yeast group comprising at least one of Saccharomyces cerevisiae, Pichia jadinii, Hanseniaspora uvarum, Cryptococcus laurentii, and Meyerozyma caribbica; A purified soil remediation agent containing microorganisms, comprising a potassium-degrading yeast group including at least one of Pichia guilliermondii, Debaryomyces hansenii, Kluyveromyces marxianus, Torulaspora delbrueckii, and Cryptococcus albidus; wherein the carbon source organic material is composed of a mixture of 55 to 65 weight% of vegetable oil, 20 to 25 weight% of bovine bone meal, and 15 to 20 weight% of fish meal; wherein the mineral raw material includes at least one of perlite, bentonite, maifan stone, diatomaceous earth, illite, zeolite, and vermiculite; wherein the functional complex yeast microbial group is immobilized inside a purified pellet in a state adsorbed on the surface of the carbon source organic material and the mineral raw material; wherein the weight moisture content of the purified pellet is 8 to 12 weight%, and the number of viable bacteria inside the purified pellet is maintained at 1.0 × 10^6 CFU / g or more. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 A step of sorting carbon source organic matter and mineral raw materials using a magnet and a sorter and feeding them into a raw material storage unit; a step of feeding a functional complex yeast microbial group into the raw material storage unit and mixing them while maintaining the internal temperature of a mixer at 30℃ or lower to adsorb the functional complex yeast microbial group onto the surface of the carbon source organic matter and the mineral raw materials; a step of feeding the mixed raw materials into a granulation machine and molding them into pellets with a diameter of 5 to 7 mm by an extrusion tableting method; blow-drying the pellets at a temperature of 35 to 45℃, wherein the weight moisture content of the pellets is adjusted to 8 to 12 weight%, and the number of viable cells in the pellets is 1.The method comprises the steps of: maintaining at least 0×10^6 CFU / g; and selecting and packaging dried pellets; wherein the functional complex yeast microbial group comprises: a nitrogen-fixing yeast group including at least one of Lipomyces starkeyi, Rhodotorula mucilaginosa, Candida tropicalis, Debaryomyces hansenii, and Metschnikowia reukaufii; and a phosphate-degrading yeast group including at least one of Saccharomyces cerevisiae, Pichia jadinii, Hanseniaspora uvarum, Cryptococcus laurentii, and Meyerozyma caribbica; A method for manufacturing a purified soil remediation agent containing microorganisms, comprising a group of potassium-degrading yeasts including at least one of Pichia guilliermondii, Debaryomyces hansenii, Kluyveromyces marxianus, Torulaspora delbrueckii, and Cryptococcus albidus; wherein the carbon source organic material is composed of a mixture of 55 to 65 weight% vegetable oil, 20 to 25 weight% bovine bone meal, and 15 to 20 weight% fish meal, and wherein the mineral raw material comprises at least one of perlite, bentonite, maifan stone, diatomite, illite, zeolite, and vermiculite. Claim 7 delete Claim 8 delete Claim 9 delete Claim 10 delete

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