Composition containing plant leaf cuticle and manufacturing method thereof

Abstract

The present invention relates to a composition containing plant leaf cuticles and a method for preparing the same. More specifically, a cuticle extract isolated and extracted from plant leaves has the effect of improving skin hydration and increasing skin gloss, and can be usefully used to improve skin condition.

Description

Technology Field

[0001] The present invention relates to a composition containing a plant leaf cuticle and a method for preparing the same. Background Technology

[0002] Among the chemical-based cosmetic ingredients commonly used, functional ingredients intended to improve skin moisture content and skin radiance have the disadvantage of causing side effects such as skin irritation or cytotoxicity, even though they are effective. Therefore, there is an increasing demand for products based on natural extracts that are non-cytotoxic, which possess the functionality to improve skin moisture content, skin radiance, and skin hydration, thereby maintaining a healthy skin condition.

[0003] Against this backdrop, the inventors conducted multifaceted research on various plant extracts containing natural substances safe for the human body as main ingredients. As a result, they confirmed that plant leaf cuticle extracts exhibit efficacy in improving skin hydration and increasing skin gloss, thereby completing the present invention. The problem to be solved

[0004] Therefore, the objective of the present invention is to provide a composition containing cuticle isolated and extracted from a plant leaf.

[0005] In addition, another objective of the present invention is to provide a method for preparing a cuticle extract for separating cuticles from plant leaves.

[0006] The various problems intended to be solved by the present invention as described above are not limited thereto, and will be clearly understood by those skilled in the art through the embodiments and claims described below. means of solving the problem

[0007] To achieve the above objective, the present invention provides a cosmetic composition containing a plant leaf cuticle extract.

[0008] Here, the plant leaf may be one or more selected from the group of plant leaves consisting of green tea leaves, camellia leaves, cedar leaves, tea tree leaves, centella leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves.

[0009] The cuticle extract of the present invention may have efficacy in improving skin hydration and skin gloss.

[0010] In the present invention, the “cuticle” is a membrane layer of cutin, a waxy substance or fatty acid, covering the surface of the body. These substances are produced in epidermal cells or the tissues within them, infiltrate the cellulose or pectin layer forming the cell wall, and then secrete and accumulate on the outside of the epidermis. It is a layer that stains well with lipid-affinity dyes, is located outside the pectin layer of the outer wall of epidermal cells, and can be separated using organic solvents. The cuticle is present on the entire surface of leaves, stems, flowers, and fruits, and becomes more developed and thicker as the organ matures. In addition to the external cuticle covering the body surface, there is also a thin membrane of waxy substance or cutin on the cell walls within the leaf flesh and epidermal tissues that come into contact with air; this is called the internal cuticle. These two cuticle layers are connected to each other through the cell surface of the stomata.

[0011] The cuticle consists of a lipophilic cutin polymer matrix and wax (Holloway PJ (1982) In The Plant Cuticle. Academic Press, London, pp 132). Very long-chain fatty acids (VLCFAs, C20–C34) and their derivatives, cuticle waxes, are interbedded and stacked within the cutin polymer matrix. The biosynthesis of cuticle wax is known to occur only in epidermal cells (Suh et al., (2005) Plant Physiol 139: 16491665). During this process, C16 and C18 fatty acids synthesized in the plastids are sent to the cytosol and extended into VLCFAs within the C20–C34 ​​carbon range by the fatty acid elongase complex in the endoplasmic reticulum (ER) (Millar AA, Kunst L (1997) Plant J 12: 121–131). The extended VLCFAs are modified through two major wax biosynthetic pathways.

[0012] In the present invention, the plant leaves of the raw materials of the composition, such as green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla leaves, chamomile leaves, ginkgo leaves, cypress leaves, mint leaves, and ginseng leaves, may be cultivated, harvested, or commercially available without limitation.

[0013] The above green tea ( Camellia sinensisGreen tea is an evergreen tree or shrub belonging to the family Theaceae. It is widely distributed in southern South Korea, including Boseong and Jeju Island, and is a plant of the genus Camellia that inhabits mainly warm regions. Its buds and leaves are primarily used for food. Green tea has been widely consumed since before Christ, and it is known that components such as catechins, theanine, vitamin C, carotenoids, and vitamin E, which are abundant in green tea, have various effects. It is also applied in various beverages, teas, alcoholic drinks, and other diverse foods.

[0014] The above camellia tree ( Camellia japonica Camellia is a plant belonging to the evergreen tree family Theaceae, and is widely distributed in southern South Korea, including Haenam, Wando, Yeosu, Geoje, and Jeju Island. Since ancient times, young camellia leaves have been used as an ingredient for tea in regions where tea trees did not grow. Its origin is Asia, and it is reported that approximately 200 species are distributed there; among them, one species exists in Korea. Camellia japonica It is naturally distributed. The camellia tree is a tree of the genus Camellia that reaches a height of 8 m and has a long oval shape, with thick, glossy leaves. Camellia leaves contain large amounts of components such as polyphenol compounds, amino acids, tannins, monosaccharides, and minerals, and their physiological activity is being studied.

[0015] The above cedar ( Cryptomeria japonica The Japanese cedar is an evergreen tree of the Coniferales order of gymnosperms and is widely distributed in the Jeju Island region and southern parts of South Korea in warm, humid, dense forests with an average annual temperature of 13℃ or higher. Japanese cedar is used for interior materials, furniture, and paint rugs, and it is known that Japanese cedar leaves contain taxifolin-type flavonoid compounds.

[0016] The above tea tree ( Melaleuca alternifolia) is an evergreen tree belonging to the Myrtaceae family of woody plants. The tea tree that grows in swamps is so vigorous that it can still grow well even if its stem is cut off. The water around the tea tree turns reddish-brown, which is thought to be similar to red tea, so people began to drink it as tea, giving rise to the name "tea tree". Tea tree oil is known as an active ingredient of the tea tree, and these ingredients are known to have effects such as treating skin wounds, antibacterial and antifungal properties.

[0017] The Asiatic pennywort (Centella asiatica) is a perennial herb that commonly grows in mountains and fields in Korea and is mainly distributed in Asian regions such as Japan and China. Recently, it has been used in the manufacture of antibacterial cosmetics because it has an antibacterial effect against bacteria, fungi, etc., but it has the disadvantage that it is difficult to expect a large antibacterial effect when used alone.

[0018] The bamboo (Bambusoideae) is a general term for perennial evergreen woody plants belonging to the Poaceae family of monocotyledonous plants. It grows wild in tropical and subtropical regions and is particularly common in the monsoon climate zone of Asia. There are more than 400 species distributed worldwide, and more than 10 species grow in Korea. Among them, Phyllostachys bambusoides ), the light bamboo ( Phyllostachys nigra ), and the Moso bamboo ( Phyllostachys pubescens ) are usefully used. Bamboo is used as a material for bamboo products and has various uses. Bamboo shoots are edible, bamboo shavings (thin inner bark) are used as traditional Chinese medicine ingredients, and bamboo essence (bamboo sap) is used as a health functional beverage or a traditional Chinese medicine ingredient.

[0019] The basil ( Ocimum basilicumBasil is an annual plant belonging to the genus *Rhamnus* of the family Lamiaceae. Due to its strong aroma, it is used as a spice or fragrance, and its leaves are primarily used in various dishes. Basil seeds are rich in various nutrients and have high levels of essential fatty acids, such as vitamin E, the antioxidant tocopherol, and eugenol. Basil oil is mainly used as a flavoring agent, but it is also utilized in pharmaceuticals and food, and recently, as a raw material for biopesticides.

[0020] The above blueberry is a shrub belonging to the genus Vaccinium of the family Ericaceae, and is a lowbush blueberry ( Vaccinium myritillus ) Highbush Blueberry( Vaccinium corymbosum ), and rabbit-eye blueberry( Vaccinium ashei There are three main types. Blueberries contain large amounts of anthocyanins and carotenoid pigments, and are known to have excellent antioxidant and anti-inflammatory effects. They are also known to promote the activation of retinal rhodopsin resynthesis, thereby reducing eye fatigue and improving vision. Among these, blueberry leaves are reported to have beneficial effects on urinary tract infections, diarrhea, and diabetes.

[0021] The above strawberry ( Fragaria ananassa The strawberry is native to the temperate regions of the Northern Hemisphere but is also widely cultivated in the Southern Hemisphere. Most cultivated varieties are variations of the Virginia strawberry and the Chilean strawberry. Strawberries have fibrous roots, and the leaves emerge from the roots; they consist of three leaflets that are hairy and have serrated edges. The flowers are almost always white, but red ones also bloom, appearing in clusters on slender flower stalks. The flower stalks resemble creeping stems.

[0022] The above vitamin tree ( Hippophae rhamnoides L.The *Sanjanamu* (sea buckthorn) is a shrub belonging to the Elaeagnaceae family, also known as *Sanjanamu*, *Galmaeborisunamu*, or *Borisu-ajaebi*. It contains over 100 types of rich active ingredients, including vitamins, flavonoids, amino acids, and trace elements. Recently, as significant interest has focused on developing functional products utilizing the excellent nutritional value and physiological activity of its fruits, leaves, and bark, its cultivation area is gradually expanding worldwide. In particular, the fruit has been reported to be rich in antioxidants such as polyphenols, tocopherols, and carotenoids. Furthermore, oil extracted from the fruit is known to boost the body's immune function and is used for skin, respiratory, and digestive system disorders. It is also known to be highly effective in treating cuts, burns, and inflammation.

[0023] The above Moringa ( Moringa oleifera Moringa is a perennial plant distributed in tropical and subtropical climates, and depending on the characteristics of each part, it is utilized in various ways, such as in pharmaceuticals, cosmetics, water purification agents, animal feed, and crop growth promoters. All parts of the Moringa—including leaves, seeds, flowers, trunks, fruits, and roots—are used as food ingredients. It has been reported that Moringa leaves have a relatively low water content compared to the leaves of other plants, resulting in a relatively higher content of various nutrients. Moringa leaves are rich not only in protein but also in minerals such as beta-carotene, vitamins, carotene, calcium, potassium, and iron.

[0024] The above aronia ( Aronia melanocarpaAronia is a berry belonging to the Rosaceae family, also known as black chokeberry. Initially, it was cultivated primarily as a source for food coloring or for ornamental purposes. Its value as a food ingredient began to be recognized in the early 1930s when a Russian botanist discovered its pleasant taste and aroma and used the juice as a beverage. The major functional components of Aronia include vitamins A, C, E, B2, B6, B9, and B12, folic acid, quinic acid, phenolic acids, anthocyanin, tannin, catechins, quercetin, rutin, hesperidin, resveratrol, and beta-carotene, as well as various organic minerals such as calcium, iron, magnesium, zinc, potassium, and manganese. Due to their physiological functions, Aronia is widely used as a functional food ingredient.

[0025] The above lemon balm ( Melissa officinalis Lemon balm is a cold-resistant perennial plant of the Lamiaceae family. Because it has a lemon scent, it is also called melisse citronnelle. The alcoholic extract of the lemon balm is used as an ingredient in l'eau des Carmes, a medicinal liquid known for its efficacy in strengthening the heart and relieving headaches. All parts of the lemon balm—flowers, leaves, and stems—can be used, and the essential oil is used as an active ingredient.

[0026] The above rosemary ( Rosmarinus officinalisRosemary (L.) is an evergreen woody plant native to the Mediterranean and belonging to the Lamiaceae family. It is one of the traditional European spices that has been cultivated mainly in southern Europe. It contains essential oils with 1,8-cineole and camphor as main components, as well as bitter components such as rosmarinic acid, and has been used medicinally as a stomachic, carminative, anti-inflammatory, and insecticide. Recently, this plant is being cultivated in large quantities in Korea for herbal bonsai, and rosemary oil is being prepared in various formulations by blending it with other types of aromas for use in aromatherapy. Meanwhile, active research is also being conducted on its application as a food preservative utilizing the antioxidant activity of rosemary components.

[0027] The above witch hazel ( Hamamelis virginiana Witch hazel is a type of deciduous tree native to North America that grows to a height of 5 to 10 meters in subtropical regions. Straight twigs emerge from curved branches, and the leaves are egg-shaped. The flowers bloom late, appearing in the autumn after the leaves have fallen. The leaves, stems, twigs, bark, and seeds of witch hazel can be utilized in various ways. It contains natural plant alcohols, including tannins, flavonoids, and various hexenols, as its main components. It is particularly useful for oily skin and skin with enlarged pores, and is known to possess activities such as antioxidant effects.

[0028] The above desert rose ( Adenium obesumIt is also called 'Seokhwa' and is a plant belonging to the Apocynaceae family of the Gentianales order; its scientific name is derived from 'Aden' in Yemen, the place where it was first discovered. It is a succulent plant with thickening stems that stores large amounts of water in its stems and leaves above ground to survive in dry, low-moisture regions such as deserts or high mountains. Research related to desert roses has reported that cardiac glycosides and pregnanes, components found in the leaves and stems of the Seokhwa plant, possess anticancer effects.

[0029] The above mugwort ( Artemisia indica Artemisia is a perennial herbaceous plant belonging to the genus Artemisia; its rhizomes grow creeping sideways, and the entire plant is covered in cobweb-like white hairs. Leaves emerging from the roots are clustered in a rosette-like arrangement, while leaves on the stem are alternate. The leaves are deeply divided into 4 to 8 lobes like a feather and are fragrant. Pale pink flowers bloom in clusters in capitulum inflorescences at the tips of the stems around July to September, with each inflorescence appearing as a single flower cluster. Commonly, in addition to Artemisia vulgaris, Artemisia annua ( A. montana ), mugwort ( A. lavandulaefolia ), mugwort ( A. rubripes Mugwort is also referred to as mugwort. People feel the spring by making soup with the young shoots that appear in early spring, roast mugwort to drink as tea, and make and eat mugwort rice cakes with mugwort added.

[0030] The above banana is a banana plant ( Musa It belongs to the genus Musa sapientum, is a perennial herb, and is cultivated in tropical to subtropical regions with temperatures above 10 degrees Celsius. It thrives in deep, soft, and well-drained soil. Musa Sapientum ) or Musa Paradisiaca L.( Musa paradisiacaThere is a banana with the scientific name *L.*. The banana fruit is an alkaline food high in calories and carbohydrates, containing potassium, carotene, and vitamin C. Fiber is obtained from the leaf stalks, and a black dye is extracted from the mucilage of the leaves. In traditional Korean medicine, bananas are believed to have properties that reduce heat and moisten the intestines, so they are used for thirst in the throat caused by heat or constipation caused by dry intestines.

[0031] The rose mentioned above is a perennial woody plant belonging to the genus Rosa of the family Rosaceae, and more than 100 species are known worldwide. Common roses are horticultural varieties ( Rosa hybrida As a Hort., the flowers (rose flowers) and fruits (rose hips) have been used for medicinal and culinary purposes. In particular, rose flowers have been used for decoration and fragrance due to their beautiful appearance and distinctive scent, while rose oil has been used in folk medicine for relieving inflammation and treating atopic dermatitis.

[0032] The above pomegranate ( Punica granatum Pomegranate (*L.*) is a plant widely distributed throughout subtropical and tropical regions, including Asia. Since ancient times, pomegranates, especially red pomegranates, have been known as tonics, and are particularly known to be effective in preventing high blood pressure and arteriosclerosis. In addition, it is known to contain a large amount of water-soluble carbohydrates, ranging from 38 to 47%, as well as various vitamins and minerals.

[0033] The above olive ( Olea europaea Linnaeus Olives are plants of the Oleaceae family, and their leaves and fruits are used for food, in accordance with the Standards and Specifications for Food. Native to the Mediterranean region, they grow mainly in hot and dry Southern Europe, such as Spain and Italy, because their leaves are small, firm, and relatively resistant to drought. Oleuropein, known to be abundant in olive leaves, is known to be effective in improving vascular health, anti-aging, anti-inflammation, and preventing constipation, while hydroxytyrosol is known to be effective against inflammation.

[0034] The above Houttuynia cordata ( Houttuynia cordata Thunb. The stem is 20–35 cm long and 2–3 mm in diameter, featuring longitudinal wrinkles and distinct nodes. Fine roots remain at the lower nodes, and the texture is soft and easily broken. The leaves are curled or shriveled, but become heart-shaped when unfolded. The leaves are 3–5 cm long and 3–4 cm wide, with pointed tips. Major components include apigilline and beta-sitosterol. Pharmacological effects include cardiotonic, diuretic, and antibacterial properties; it acts on the lungs and possesses antipyretic and detoxifying effects, as well as the ability to eliminate boils and remove pus. Therefore, it is known to be effective against all types of heat toxins and boils.

[0035] The above perilla leaf ( Perilla frutescens var. acuta Perilla is a herbaceous plant belonging to the Lamiaceae family, reaching a height of 30 to 60 cm. It is also known as red perilla, shiso, and wrinkled shiso. It is produced in Korea, China, and other regions, where it is used for both culinary and medicinal purposes. Perilla leaves are purple in color and possess a distinctive flavor; the stems are straight and angular, while the leaves have rounded or wedge-shaped bases and serrated edges. The flowers are pale purple and bloom in racemes around August to September. The shape of the leaves and stems is similar to that of sesame. Its major components are known to include perilla aldehyde, d-limonene, α-pinene, cyanin, sterin, menthol, rosmarinic acid, and luteolin. Research suggests that consuming it with meat or fish can prevent food poisoning and possesses strong antibacterial properties.

[0036] The above chamomile ( Chamomilla recutitaChamomile is a herb belonging to the Asteraceae family, order Campanulales, and class Dicotyledon. It is also known by names such as chamomile, chamomile, or chamomile. Chamomile is said to have excellent sedative properties that help alleviate various types of pain and soothe skin troubles. It is also said to be effective in treating redness caused by stress and dryness, as well as hypersensitive skin. The essential oil content in the flowers ranges from 0.05% to 1.9% depending on cultivation conditions, but generally ranges from 0.2% to 0.9%. The essential oil is blue in color. The composition of the essential oil consists of approximately 4.6% camazulene (an azulene compound), about 10% terpene compounds and sesquiterpene compounds, about 20% sesquiterpene alcohol compounds, cadinene, caprylic acid, nonyl acid, and isovaleric acid. In addition, flavonoids include affine, quercimerythrin, luteolin-7-glucoside, paturithrin, matricin, and matricarin.

[0037] The above bank ( Ginkgo biloba L) contains various components in its leaves. The ginkgo leaves contain flavonoids, biflavonoids, proanthocyanidins, and terpene lactones as major components, and bioflavonoids such as quercetin, kaempferol, and isohamnetin are known to be abundant when the most mature green leaves are harvested in September or October before falling off. In addition, the ginkgo leaf extract is known to inhibit the expression of melasma-like blood vessels, which are found particularly frequently in melasma areas, thereby preventing the abnormal activation of melanocytes.

[0038] The above cypress ( Chamaecyparis obtusa It is mostly distributed in the Asian region and is also called Japanese cypress or cypress. Cupressaceae ( CuperssaceaeIt is an evergreen scale-leaved tree belonging to the family, native to Japan. The above-mentioned Hinoki cypress is effective for nourishing and strengthening the body, treating diarrhea, stopping bleeding, treating rheumatism, neuralgia, hemoptysis, intestinal bleeding, and strengthening the body. It is also known that the leaves of the above-mentioned Hinoki cypress contain a large amount of phytoncide, which helps with psychological stability, strengthening cardiopulmonary function, and strengthening the heart.

[0039] The above peppermint ( Mentha arvensis Peppermint (*var. piperascens*) is a perennial herbaceous plant belonging to the Lamiaceae family of the order Lamiales. It possesses an aromatic quality, grows upright, and branches from the top. Major components of peppermint include alpha-pinene, anisaldehyde, daucosterol, eugenol, linalool, menthone, and neomenthol. It is known to possess bactericidal and antimicrobial effects, improve skin diseases, soothe the skin, and have antioxidant properties. Menthol, the main component of peppermint oil, is used medicinally as a topical agent, analgesic, stimulant, or stomach tonic, and is also used as a flavoring or refreshing agent in toothpaste, candy, jam, cosmetics, and tobacco.

[0040] The above ginseng ( Panax ginseng Ginseng (C.A. Meyer) is a perennial plant belonging to the genus *ginseng* of the family *Araliaceae*. In Korea and Japan, it is known to be produced by removing the roots and cork layer. In particular, in Korea, ginseng is classified into white ginseng (fresh ginseng), red ginseng (steamed ginseng), and black ginseng (three-steaming and nine-drying), and these products are produced and distributed accordingly. Ginseng has been used as a traditional medicinal herb for thousands of years in Asian countries such as Korea, China, and Japan, and in recent years, it has been consumed globally. With the functional properties of its components established, it is being utilized in a wide variety of fields.

[0041] The above plants may preferably be selected from the group including the flowers, leaves, stems, fruits, seeds, roots, and whole plants of the plants, more preferably from the group including the leaves, flowers, and stems of the plants, and most preferably from the leaves of the plants. Through the examples described below, the functionality of the cuticle present on the surface of the above green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves has been demonstrated.

[0042] The term “extract” as used in the present invention refers to a product obtained by extracting a subject of extraction with a suitable extraction solvent, and may include an extract obtained by an extraction process, a diluted or concentrated extract, a dried product obtained by drying an extract, or a purified product or a fraction that can be obtained by purifying or fractionating the extracts.

[0043] Preferably, the plant leaf cuticle extract may be obtained by passing through the steps of steaming dried plant leaves with steam and extracting the steamed plant leaves prepared in the steaming step with a solvent. In the present invention, it is desirable to ensure that the plant leaf and the surface cuticle are well separated through the steaming process, and that the cuticle can be easily extracted in the subsequent solvent extraction process.

[0044] The extract according to the present invention can be prepared using general extraction methods known in the art, for example, hot water extraction, hot water extraction, cold maceration extraction, reflux cooling extraction, ultrasonic extraction, high temperature and high pressure extraction.

[0045] The extraction solvent used to prepare the extract according to the present invention may be one or more selected from the group consisting of water, ethanol, methanol, butanol, methylpropanediol, propanediol, butylene glycol, hexanediol, propylene glycol, pentylene glycol, chloroform, ethyl acetate, dichloromethane, hexane, petroleum ether, vegetable oil, mineral oil, and diethyl ether, and the extraction solvent may be used in an amount of 0.01 to 100 times the weight of the total extract.

[0046] The extract according to the present invention may include not only the extract obtained by the extraction solvent described above, but also the extract obtained by undergoing conventional purification, enzymatic treatment, and fermentation processes. For example, the extract may also include the extract obtained through various additional purification and extraction methods, such as extraction using reduced pressure with carbon dioxide, high temperature or ultrasound, or extracts obtained from fermentation products in a natural state or using various microorganisms.

[0047] In addition, according to an embodiment of the present invention, a skin external preparation composition containing a plant leaf cuticle extract is provided.

[0048] The content of the extract included as an active ingredient in the cosmetic composition or external skin preparation according to the present invention can be appropriately determined by considering various factors such as the purpose of use, duration of use, type of formulation, route of administration, and skin condition of the subject. For example, the content of the plant leaf cuticle extract may be 0.0001 to 100 weight%, e.g., 0.01 to 50 weight%, based on the total weight of the cosmetic composition.

[0049] The composition according to the present invention can be prepared in the form of a general emulsion formulation and a solubilized formulation, etc., using a commonly known manufacturing method. At this time, the cosmetic composition of the present invention can be prepared in various formulations such as patches, ointments, skin adhesive gels, creams, packs, lotions, essences, sprays, masks, foundations, makeup bases, cleansers, water (W) type, oil (O) type, silicone (S) type, oil in water (O / W) type, water in oil (W / O) type, silicone in water (W / S) type, silicone in water (S / W) type, solid phase, liquid phase, etc., and a commonly used cosmetic manufacturing method may be applied.

[0050] The composition according to the present invention may further include additional ingredients in addition to the extract to enhance efficacy. For example, there are no limitations on the additional ingredients provided they do not offset or reduce the efficacy of the extract. Optionally, adjuvants, carriers, etc., commonly used in the field of cosmetics may be further included. Optionally, ingredients typically used to add or enhance cosmetic functions may also be added. For example, one or more aqueous additives selected from stabilizers, emulsifiers, thickeners, moisturizers, liquid crystal film strengtheners, pH adjusters, antimicrobial agents, water-soluble polymers, coating agents, metal ion chelating agents, amino acids, organic amines, polymer emulsions, pH adjusters, skin nutrients, antioxidants, antioxidant aids, preservatives, fragrances, etc.; and one or more oily additives selected from oils, waxes, hydrocarbon oils, higher fatty acid oils, higher alcohols, synthetic ester oils, silicone oils, etc.

[0051] As described above, the plant leaf cuticle extract according to the present invention can exhibit effects of improving skin hydration and skin gloss. Accordingly, the composition of the present invention containing the extract can be applied as an external skin preparation composition capable of improving skin condition.

[0052] The composition according to the present invention provides the efficacy of increasing skin moisture and skin gloss after application to the skin, and can increase the moisture content of the skin by increasing the production of Aquaporin-3 (AQP-3).

[0053] The composition according to the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. This may be provided in any formulation suitable for topical application, for example, in the form of a solution, gel, solid, paste anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule, or ionic (liposome) and non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray, or concealer stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

[0054] Preferably, the cosmetic product may have any one formulation selected from the group consisting of softening lotion, astringent lotion, nourishing lotion, essence, pack, patch, cream, skin adhesive gel, foundation, or makeup base.

[0055] In addition, the composition according to the present invention may contain adjuvants commonly used in the field of cosmetics or dermatology, such as fatty substances, organic solvents, solvents, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion chelating agents, chelating agents, preservatives, vitamins, blockers, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, or any other ingredients commonly used in cosmetics. The adjuvants are introduced in amounts commonly used in the field of cosmetics or dermatology.

[0056] According to another embodiment of the present invention, a food composition comprising a plant leaf cuticle extract is provided.

[0057] The above food may be a health functional food. The term "health functional food" refers to a food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc., using raw materials or ingredients that have functional properties useful to the human body. Here, "functionality" can be interpreted as obtaining useful effects for health purposes, such as regulating nutrients or physiological actions regarding the structure and function of the human body.

[0058] The food composition according to the present invention can be prepared by methods commonly used in the art, and can be prepared by adding raw materials and ingredients commonly added in the art during preparation. In addition, since the food composition according to the present invention contains natural plant extracts as active ingredients, there are no side effects or tolerances that may generally occur with long-term use. Therefore, the food composition according to the present invention can be used alone, and it can also be used simultaneously or sequentially with a cosmetic composition, pharmaceutical composition, quasi-drug composition, or other composition or other therapy according to the present invention for the purpose of maximizing the effect of improving skin condition.

[0059] The content of the extract included as an active ingredient in the food composition according to the present invention can be appropriately determined according to the purpose of use, duration of use, skin condition of the subject, etc.

[0060] The food composition according to the present invention may further include additional ingredients in addition to the active ingredient to enhance efficacy. For example, the additional ingredient may be an ingredient or preparation that exhibits a skin condition improvement effect, provided that it does not offset or reduce the efficacy of the extract according to the present invention.

[0061] The above food composition may be in the form of pills, tablets, granules, powders, capsules, liquids, etc. In addition, the types of the above food are not particularly limited. For example, foods that may contain the above extract include meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, vitamin complexes, etc., and include all foods in the conventional sense.

[0062] The food composition of the present invention may contain various flavoring agents or natural carbohydrates used in conventional foods as additional ingredients. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As a sweetener, natural sweeteners such as taumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. Effects of the invention

[0063] As described above, the composition containing the plant leaf cuticle extract according to the present invention can be usefully applied in various fields requiring improvement of skin condition by providing efficacy in improving skin moisturization and skin gloss.

[0064] In addition, according to the method for extracting cuticle from a plant leaf of the present invention, the cuticle present on the surface of the plant leaf can be extracted with a high yield.

[0065] The effects according to the present invention described above are not limited to those stated above, and other effects of the present invention will be clearly understood by those skilled in the art through the embodiments and claims described below. Brief explanation of the drawing

[0066] Figure 1 is the result of gas chromatography component analysis of an extract prepared by the steaming extraction method of the present invention. Figure 2 is the result of gas chromatography component analysis of an extract prepared by the hot water extraction method of the present invention. Figure 3 is the result of gas chromatography component analysis of an extract prepared by the ultrasonic extraction method of the present invention. Figure 4 is the result of gas chromatography component analysis of an extract prepared by the ultra-high pressure extraction method of the present invention. Figure 5 shows the standard indicators for gas chromatography component analysis of the present invention. Specific details for implementing the invention

[0067] To aid in understanding the present invention, specific examples will be described below. However, the following examples are intended to explain the invention more specifically and should not be interpreted as limiting the invention, and ordinary variations by those skilled in the art are possible within the scope of the invention.

[0068] Furthermore, unless otherwise specifically limited, terms, technologies, etc. described in this specification should be understood in the sense commonly used in the technical field to which this invention belongs.

[0069] Example: Steamed Extract

[0070] Cleanly washed natural product samples—green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla frutescens leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves—were each dried. 60g of each dried natural product sample was steamed for 3 hours using steam distillation equipment. Subsequently, static extraction was performed using 540g of butylene glycol solvent for 24 hours.

[0071] After the extraction process was completed, the raw material and extract were separated by centrifuging using a centrifuge (Supra 22K, Hanil, Korea) at 8,000 rpm and 15°C for 20 minutes. Accordingly, the separated extract was sequentially filtered through 0.8 μm and 0.45 μm filters. The filtrate obtained in this way was used in the test examples described below.

[0073] Comparative Example 1: Ultra-high pressure extraction

[0074] Cleanly washed natural product samples—green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla frutescens leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves—were each dried. 60g of each dried natural product sample was placed in a vacuum bag with 540g of butylene glycol solvent, and the immersed samples were vacuum-treated using a vacuum machine (IS-100, Namwoong, Korea). Next, double liquid-phase ultra-high pressure treatment was performed for 24 hours under conditions of 100 MPa (1,000 bar), 25°C, and water (buffer solvent) using an ultra-high pressure device (TFS-2L, TOYO KOATSU CO., Ltd., JPN).

[0075] After the extraction process was completed, the raw material and extract were separated by centrifuging using a centrifuge (Supra 22K, Hanil, Korea) at 8,000 rpm and 15°C for 20 minutes. Accordingly, the separated extract was sequentially filtered through 0.8 μm and 0.45 μm filters. The filtrate obtained in this way was used in the test examples described below.

[0077] Comparative Example 2: Ultrasonic extraction

[0078] Cleanly washed natural product samples—green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla frutescens leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves—were each dried. 60g of each dried natural product sample was placed in a beaker with 540g of butylene glycol solvent and subjected to ultrasonic extraction treatment for 24 hours using an ultrasonic device (5510R-DTH, BARANSON, USA) under conditions of 20kHz, 750W, and 25℃ while cooling the bath.

[0079] After the extraction process was completed, the raw material and extract were separated by centrifuging using a centrifuge (Supra 22K, Hanil, Korea) at 8,000 rpm and 15°C for 20 minutes. Accordingly, the separated extract was sequentially filtered through 0.8 μm and 0.45 μm filters. The filtrate obtained in this way was used in the test examples described below.

[0081] Comparative Example 3: Hot water extraction

[0082] Cleanly washed natural product samples—green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla frutescens leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves—were each dried. 60g of each dried natural product sample was placed in a round-bottom flask with 540g of butylene glycol solvent and subjected to hot water (reflux) extraction at 80℃ for 1 hour in an extractor equipped with a cooling condenser.

[0083] After the extraction process was completed, the raw material and extract were separated by centrifuging using a centrifuge (Supra 22K, Hanil, Korea) at 8,000 rpm and 15°C for 20 minutes. Accordingly, the separated extract was sequentially filtered through 0.8 μm and 0.45 μm filters. The filtrate obtained in this way was used in the test examples described below.

[0085] Test Example 1: Extraction Yield

[0086] In this test example, the cuticle extraction yield according to the extraction method was verified.

[0087] The filtrate obtained in each of the above examples and comparative examples was subjected to a drying weight lossr (HC103, METTLER TOLEDO, USA) to remove the solvent, butylene glycol, at 210°C for 3 hours, and the amount of the remaining cuticle paste solid was calculated. The extraction yield (%) was calculated using the formula: amount of solid obtained (g) / amount of input raw material (g) x 100.

[0088] The extraction yield (%) calculated for each natural product in the examples and comparative examples is shown in Table 1 below. In Table 1, "-" means less than 0.1%.

[0089] Comparative Example 1 (Hot water extraction method, %) Comparative Example 2 (ultrasonic extraction method, %) Comparative Example 3 (Ultra-high pressure extraction method, %) Examples (Steaming Extraction Method, %) green tea 0.13 0.22 0.59 3.62 Camellia 0.27 0.35 0.30 2.14 cedar 0.11 - 0.44 3.49 Tea tree - - 0.24 2.61 Centella asiatica - - 0.36 1.85 bamboo - - 0.23 1.27 basil - - 0.21 2.37 blueberries - - - 1.76 strawberry - - - 2.53 Vitamin tree - - - 1.96 Moringa - - - 3.30 Aronia - - - 1.61 Lemon balm - - - 2.64 rosemary - - - 1.98 Witch hazel - - - 0.97 Desert rose - - - 1.33 mugwort - - - 0.56 banana 0.21 0.17 0.33 2.70 rose - - - 2.51 pomegranate - - - 1.53 olive - - - 2.22 Houttuynia cordata - - - 1.64 Perilla leaf - - - 0.87 Chamomile - - - 0.13 bank - - - 0.53 Hinoki cypress - - - 0.47 mint - - - 1.33 ginseng - - - 2.18

[0091] Test Example 2: Qualitative Verification of Cuticle

[0092] In this test example, cuticle extraction was qualitatively confirmed.

[0093] Qualitative analysis of fatty acids, which are one of the components of the cuticle, was performed on green tea samples obtained in the above examples and comparative examples, respectively, using GC-FID (Agilent, USA) under temperature gradient conditions. The column used in the experiment was the DB-FATWAX Ultra Inert GC Column (Agilent, USA), and the analysis results are shown in Figures 1 to 5.

[0094] The cuticle is composed primarily of lipids, and gas chromatography analysis allows for the analysis of lipid layers ranging from those with a small carbon number (light) to those with a large carbon number (heavy). The lipid layer with a small carbon number is analyzed starting from the side with the shortest retention time on the left, while the lipid layer with a large carbon number is analyzed and detected up to the side with the longest retention time.

[0095] As shown in FIGS. 1 to 5, cuticle components were not identified in the hot water, ultrasonic, and ultra-high pressure extraction methods, whereas in the steam extraction method of the example, cuticle components with carbon atoms 4, 6, 8, 11, 12, 13, 15, 16, 17, 22, and 24 were identified, and in particular, it was confirmed that lipid layers with relatively small carbon atoms were effectively extracted.

[0097] Test Example 3: Component Identification and Content Experiment

[0098] The total phenolic content and total flavonoid content of phenolic substances and flavonoids related to antioxidant effects among the components of each individual extract according to the extraction method of the present invention were confirmed. Specifically, the test group was subjected to comparative analysis of green tea leaf and camellia leaf extracts from the examples and comparative examples.

[0099] The total phenol content was measured as follows. 10 mL of distilled water was added to 1 mL of extract at each concentration, followed by the addition of 2 mL of Folin-Ciocalteu phenol reagent (Sigma) and mixing. The mixture was then reacted at room temperature for 5 minutes. 2 mL of 20% sodium carbonate was added to the reaction mixture and mixed. After reacting at room temperature for 1 hour, the absorbance was measured at 680 nm using a microplate reader (UVT-06685, Thermomax, USA). Gallic acid (Sigma, USA) was used as the indicator substance. A calibration curve was constructed for each concentration of the indicator substance, and the absorbance values ​​of each sample were applied to it, as shown in Table 2 below.

[0100] Total phenolic content (gallic acid equivalents, g / g of extract) Comparative Example 1 (Hot Water Extraction Method) Comparative Example 2 (Ultrasonic Extraction Method) Comparative Example 3 (Ultra-high pressure extraction method) Example (Steaming Extraction Method) green tea 0.04 0.10 0.21 0.01 Camellia 0.10 0.21 0.17 0.02

[0102] The total flavonoid content was measured as follows. 1.5 mL of extract at each concentration was mixed with an equal amount of 2% AlCl3·6H2O dissolved in methanol, reacted at room temperature for 10 minutes, and then the absorbance was measured at 367 nm using a microplate reader (UVT-06685, Thermomax, USA). Catechin (catechin, Sigma, USA) was used as the indicator substance, and a calibration curve was constructed for each concentration of the indicator substance. The absorbance values ​​of each sample were then applied to the curve and are shown in Table 3 below.

[0103] Total flavonoid content (catechin equivalents, g / g of extract) Comparative Example 1 (Hot Water Extraction Method) Comparative Example 2 (Ultrasonic Extraction Method) Comparative Example 3 (Ultra-high pressure extraction method) Example (Steaming Extraction Method) green tea 0.03 0.09 0.19 0.01 Camellia 0.09 0.18 0.16 0.02

[0104] As shown in Tables 2 and 3, the components extracted by hot water, ultrasonic, or ultra-high pressure extraction methods are mostly low molecular weight cyclic compounds such as flavonoids or polyphenols, but the steam extraction method of the example extracts plant cuticles, so it was confirmed that the extracted components are different.

[0106] Test Example 4: Moisturizing and Elasticity Effect Experiment

[0107] To compare the moisturizing and elasticity effects of each extract according to the extraction method of the present invention, a corneometric assay was performed using a skin analyzer system (MC88, MEICET, CN). The subjects of the study were healthy individuals with no history of allergic diseases. For at least one week prior to the test, no corticosteroids, antihistamines, or anti-inflammatory drugs were taken, and no topical corticosteroids were applied to the test site. As a control group, a 0.5% beta-glucan solution, known for its excellent moisturizing and elasticity effects, was used. The results are shown in Tables 4 and 5 below.

[0108] Moisturizing effect after 120 minutes (Moisture contents, %) Comparative Example 1 (Hot Water Extraction Method) Comparative Example 2 (Ultrasonic Extraction Method) Comparative Example 3 (Ultra-high pressure extraction method) Example (Steaming Extraction Method) green tea 37.9 ± 2.2 34.3 ± 5.8 35.2 ± 3.6 52.3 ± 4.4 Camellia 36.4 ± 3.0 35.7 ± 2.7 38.8 ± 4.9 57.4 ± 1.1 Control (No Coat) 35.3 ± 1.3 Control group (β-Glucan 0.5% Solution) 48.7 ± 3.7

[0109] Elasticity after 120 minutes (Elasticity, AU) Comparative Example 1 (Hot Water Extraction Method) Comparative Example 2 (Ultrasonic Extraction Method) Comparative Example 3 (Ultra-high pressure extraction method) Example (Steaming Extraction Method) green tea 4.8 ± 0.6 5.1 ± 0.4 5.2 ± 0.8 6.9 ± 0.7 Camellia 4.3 ± 0.2 5.7 ± 1.9 5.5 ± 1.3 7.2 ± 0.3 Control (No Coat) 5.1 ± 1.4 Control group (β-Glucan 0.5% Solution) 6.2 ± 1.1

[0111] Test Example 5: Skin Gloss Effect Experiment

[0112] To compare the skin gloss effects of each extract according to the extraction method of the present invention, skin gloss was measured using a Skin Glossy meter (MPA-GL200, C+K, DE). The subjects of the study were healthy individuals with no history of allergic diseases. For at least one week prior to the test, no corticosteroids, antihistamines, or anti-inflammatory drugs were taken, and no topical corticosteroids were applied to the test site. The results are shown in Table 6 below.

[0113] Gloss Effect (Gloss Value, AU) Comparative Example 1 (Hot Water Extraction Method) Comparative Example 2 (Ultrasonic Extraction Method) Comparative Example 3 (Ultra-high pressure extraction method) Example (Steaming Extraction Method) green tea 2.88 ± 0.42 3.03 ± 0.87 3.01 ± 0.54 4.9 ± 0.13 Camellia 2.93 ± 0.64 2.99 ± 0.91 3.16 ± 1.64 5.3 ± 0.51 Control (No Coat) 2.82 ± 0.78

[0115] Test Example 6: Aquaporin-3 Expression Experiment

[0116] To confirm the effect of the extract according to the extraction method of the present invention on promoting aquaporin-3 protein production, an ELISA (Enzymelinked immunosorbent assay) experiment was conducted.

[0117] Aquaporin-3 (AQP-3) is a water channel protein that selectively controls the movement of water across cell membranes, and a total of 13 types of aquaporins are known to exist in the skin. Among them, aquaporin-3 is known to block foreign substances from entering the skin, participate in the transport of water and glycerol, and supply moisture, thereby helping to strengthen the skin barrier and promote self-regeneration of moisture.

[0118] Specifically, human keratinocyte cell line HaCaT cells in a 6-well plate at a rate of approximately 2.5 x 10 5 After treatment per well, the cells were incubated in DMEM, 10% FBS, and 1% penicillin-streptomycin medium at 37°C and 5% CO2 for 24 hours. After 24 hours, the medium used for the previous culture was removed, and each sample was added to the medium and incubated at 37°C and 5% CO2 for 3 hours.

[0119] After 3 hours, cells were harvested and lysed with RIPA buffer, and quantitative analysis of aquaporin-3 expression levels was performed using an ELISA kit (Elabscience). The expression levels (%) obtained from the above experiment were compared with the untreated control group and are shown in Table 7 below.

[0120] Aquaporin 3 expression level (% of control) Comparative Example 1 (Hot Water Extraction Method) Comparative Example 2 (Ultrasonic Extraction Method) Comparative Example 3 (Ultra-high pressure extraction method) Example (Steaming Extraction Method) green tea 102.3 ± 3.3 103.4 ± 3.6 105.8 ± 4.2 142.7 ± 3.9 Camellia 103.2 ± 4.7 102.7 ± 5.7 103.6 ± 0.8 123.4 ± 1.8 Control (No Coat) 100.0 ± 4.2

[0121] As shown in Table 7, it was confirmed that the expression amount of aquaporin-3 in the extract of the example increased by up to 42.7% compared to the control group.

[0123] Cosmetic preparation example

[0124] Hereinafter, toners, lotions, creams, essences, and packs are exemplified as formulations of the present invention, but the formulations of the cosmetic composition of the present invention should not be interpreted as being limited thereto, and ordinary variations by those skilled in the art, such as food, health functional food, and pharmaceuticals, are possible within the scope of the present invention.

[0126] Cosmetic Preparation Example 1. Lotion Composition

[0127] number Ingredients Content (%) 1 purified water Remaining amount 2 Butylene glycol 5.00 3 Example composition 2.00 4 glycerin 4.00 5 Disodium EDTA 0.02 6 Carbomer 0.10 7 Betaine 0.50 8 Hyaluronic acid 0.01 9 ethanol 4.00 10 PEG-60 Hydrogenated Castor Oil 0.30 11 Methylparaben 0.10 12 Phenyltrimethicone 0.05 13 Flavoring a very small amount 14 coloring agent a very small amount

[0129] Cosmetic Preparation Example 2. Lotion Composition

[0130] number Ingredients Content (%) 1 purified water Remaining amount 2 Methylparaben 0.20 3 Example composition 5.00 4 glycerin 5.00 5 Butylene glycol 7.00 6 Carbomer 0.10 7 Xanthan sword 0.05 8 cetearyl alcohol 3.00 9 Glyceryl stearate 0.50 10 Propylparaben 0.10 11 mineral oil 3.00 12 Vegetable squalane 3.00 13 Cetyl ethylhexanoate 3.00 14 Arginine 0.10 15 flavoring agent a very small amount

[0132] Cosmetic Preparation Example 3. Cream Composition

[0133] number Ingredients Content (%) 1 purified water Remaining amount 2 Methylparaben 0.20 3 Example composition 5.00 4 glycerin 5.00 5 Butylene glycol 7.00 6 Carbomer 0.20 7 Xanthan sword 0.08 8 cetearyl alcohol 3.00 9 Glyceryl stearate 0.50 10 Behenyl alcohol 2.00 11 Hydrogen lecithin 0.20 12 Propylparaben 0.10 13 mineral oil 3.00 14 Vegetable squalane 3.00 15 Cetyl ethylhexanoate 3.00 16 Arginine 0.20 17 flavoring agent a very small amount

[0135] Cosmetic Preparation Example 4. Essence composition

[0136] number Ingredients Content (%) 1 purified water Remaining amount 2 Methylparaben 0.20 3 Example composition 5.00 4 Hyaluronic acid 0.05 5 Butylene glycol 2.00 6 Carbomer 0.08 7 Xanthan sword 0.04 8 cetearyl alcohol 0.50 9 Glyceryl stearate 0.50 10 Propylparaben 0.10 11 mineral oil 1.00 12 Vegetable squalane 2.00 13 Cetyl ethylhexanoate 1.00 14 Arginine 0.08 15 ethanol 4.00 16 PEG-60 Hydrogenated Castor Oil 0.30 17 flavoring agent a very small amount

[0138] Cosmetic Preparation Example 5. Pack Composition

[0139] number Ingredients Content (%) 1 purified water Remaining amount 2 glycerin 10.00 3 Example composition 5.00 4 Butylene glycol 5.00 5 kaoline 4.00 6 Caprylic / Capric Triglyceride 4.00 7 Cyclomethicone 1.00 8 Magnesium aluminum silicate 1.00 9 PEG-100 Stearate, Glyceryl Stearate 2.00 10 Xanthan sword 0.10 11 ethanol 3.00 12 Methylparaben 0.20 13 Chlorphenesin 0.10 14 titanium dioxide 0.50 15 Flavoring a very small amount

Claims

Claim 1 A method for extracting cuticle from a plant leaf, comprising: a step of steaming the plant leaf with steam; and a step of extracting the steamed plant leaf with a butylene glycol solvent. Claim 2 A method for extracting cuticle from a plant leaf according to claim 1, wherein the plant leaf is one or more selected from the group of plant leaves consisting of green tea leaves, camellia leaves, cedar leaves, tea tree leaves, Centella asiatica leaves, bamboo leaves, basil leaves, blueberry leaves, strawberry leaves, sea buckthorn leaves, moringa leaves, aronia leaves, lemon balm leaves, rosemary leaves, witch hazel leaves, desert rose leaves, mugwort leaves, banana leaves, rose leaves, pomegranate leaves, olive leaves, Houttuynia cordata leaves, Perilla leaves, chamomile leaves, ginkgo leaves, cypress leaves, peppermint leaves, and ginseng leaves. Claim 3 A cosmetic composition comprising a cuticle extracted according to the method of claim 1. Claim 4 In paragraph 3, the cosmetic composition is used for improving skin hydration or skin radiance. Claim 5 A food composition comprising a cuticle extracted according to the method of claim 1. Claim 6 delete Claim 7 delete Claim 8 delete Claim 9 delete Claim 10 delete

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