A cosmetic composition with excellent antioxidant, whitening, anti-inflammatory and regenerative effects

Abstract

The present invention relates to a method for preparing sesame seed meal extract and a cosmetic composition having excellent antioxidant, whitening, anti-inflammatory, and regenerative effects comprising sesame seed meal extract as an active ingredient.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition comprising sesame meal extract, and more specifically, to a cosmetic composition having excellent antioxidant, whitening, anti-inflammatory, and regenerative effects. Background Technology

[0003] The skin is the most basic defense organ that protects the body from external stimuli by coming into contact with the external environment, and it is an important organ that expresses external beauty.

[0004] As people's outdoor activities increase, prolonged exposure to sunlight damages the skin. Long-term exposure to UV rays accelerates skin aging and causes the skin to darken due to melanin pigment produced to protect the inner layers of the skin from UV rays.

[0005] Therefore, the consumption of functional cosmetics as a method of skin care is increasing in modern society, and because there is a preference for skin that is white, clean, youthful, and elastic like jade, the cosmetics industry is actively developing products for whitening and anti-aging effects. In addition, there is a growing need for multifunctional cosmetics that possess antioxidant, redness, and regenerative functions along with whitening functions.

[0006] Recently, research has continued to identify whitening active ingredients among natural products, and it has been revealed that numerous plant extracts inhibit melanin production by acting on tyrosinase. However, these also present many problems regarding their stability, safety, and potential for discoloration when used in cosmetics or pharmaceuticals at concentrations above the effective level, and they are currently unable to produce satisfactory results.

[0007] In particular, when these whitening compositions are used in combination with beauty packs, the nature of the packs maintains direct contact with the skin for a certain period; consequently, they may damage delicate skin or have inferior effects in regenerating it into healthy, radiant skin. Furthermore, synthetic beauty pack compositions have the disadvantage of potentially causing side effects such as skin rashes or allergies in severe cases. Therefore, there has recently been an increasing number of attempts to utilize natural ingredients in beauty pack products that offer both moisturizing and excellent whitening effects.

[0008] Meanwhile, the factor most closely associated with wrinkles is the aging of skin cells. Generally, the causes of skin aging can be classified into natural aging, which occurs with the aging of the population, and internal aging, which is caused by the external environment. Natural aging is difficult to control because it involves significant genetic factors. Therefore, much research is being conducted to control environmental factors such as ultraviolet radiation, oxidation, and dryness.

[0009] Environmental factors primarily cause wrinkles by destroying or denaturing collagen and elastin proteins contained in the dermis. In particular, the sun's ultraviolet rays are a representative environmental factor that oxidizes the skin and generates reactive oxygen species. This oxidizing power and reactive oxygen species can cause protein destruction, sugar oxidation, and genetic mutations within skin cells, thereby leading to skin aging and wrinkles.

[0010] Accordingly, there is a demand for the development of substances that can maximize both skin whitening and anti-wrinkle effects while minimizing the aforementioned side effects. Furthermore, there is an increasing demand for cosmetic compositions that can be used without limitations on content due to their low risk factors, such as skin irritation and cytotoxicity.

[0011] It has been reported that sesame meal contains lignan components such as sesamolinol, sasaminol, sasamin, and pinoreisnol, and thus has antioxidant effects (Namiki M. The chemistry and physiological functions of sesame, 1995, Food Rev. Intern., 11, 281-329).

[0012] Meanwhile, a method for manufacturing pork feed containing sesame meal and sesame meal using sesame meal has been disclosed in Korean Registered Patent No. 100424407, and a method for manufacturing an angiogenesis promoter containing sesamin as an active ingredient has been disclosed in Korean Registered Patent No. 10-1124621. In addition, lignan powder derived from sesame oil or sesame meal and a method for preventing oxidation by adding said lignan powder to meat and meat processing products have been disclosed in Korean Registered Patent No. 10-0414185.

[0013] In addition, Korean Patent Publication No. 10-2015-0047987 describes an antioxidant functional food composition and a feed additive composition containing sesame meal extract as an active ingredient, relating to the use of sesame meal extract.

[0014] However, there has been no disclosure to date regarding the evaluation of the antioxidant, anti-inflammatory, and regenerative efficacy of sesame meal extracts and the provision of a functional cosmetic composition using sesame meal.

[0015] Accordingly, the inventors have arrived at the present invention to provide a cosmetic composition that has a high skin whitening effect, uses natural substances so there are no side effects on the human body, particularly on the skin, and can be used as a general functional cosmetic composition, while also possessing excellent antioxidant, anti-inflammatory, and regenerative efficacy. The problem to be solved

[0017] Therefore, the objective of the present invention is to provide a cosmetic composition containing sesame meal extract as an active ingredient that has excellent antioxidant, whitening, anti-inflammatory, and regenerative effects. means of solving the problem

[0019] One embodiment of the present invention relates to a method for preparing a sesame meal extract, comprising the steps of: drying sesame seeds, cold-pressing them at 20°C to 45°C to extract oil, and obtaining the remaining sesame meal; adding and mixing the sesame meal with a solvent selected from water, a hydrophilic organic solvent, or a mixture thereof to obtain a mixture; and

[0020] The present invention provides a method comprising the step of obtaining a sesame meal extract by performing the above-described reflux extraction method.

[0021] The above mixture may contain 1 to 20 w / v% of sesame meal.

[0022] Sesame meal extract can be obtained by refluxing the above mixture for 1 to 8 hours in a reflux cooling device set to 40°C to 100°C.

[0023] Another embodiment of the present invention provides a sesame seed meal extract prepared according to the above method.

[0024] Another embodiment of the present invention provides a cosmetic composition having antioxidant, whitening, anti-inflammatory, and regenerative effects. Effects of the invention

[0026] The present invention provides a method for producing a sesame meal extract with a high effective content of sesamin and sesamolin.

[0027] The present invention provides a cosmetic composition having excellent antioxidant, whitening, anti-inflammatory, and regenerative effects, containing sesame meal extract as an active ingredient. Brief explanation of the drawing

[0029] Figure 1 is a graph measuring the melanin synthesis rate (%). Figure 2 is a graph measuring the tyrosinase activity rate (%). Figure 3 is a graph measuring the PGE2 expression inhibition rate (%). Figure 4 is an observed image of cell migration. Figure 5 is the result of testing whether pigmentation occurs when using an essence containing the sesame seed meal extract of the present invention. Specific details for implementing the invention

[0030] The following description of the present invention with reference to the drawings is not limited to specific embodiments and may be subject to various modifications and have various embodiments. Furthermore, it should be understood that the content described below includes all modifications, equivalents, and substitutions that fall within the spirit and scope of the present invention.

[0031] The present invention will be described in more detail below according to the embodiments. However, the following embodiments are merely for illustrating the present invention and are not intended to limit the scope of the present invention.

[0032] In the present invention, "5w / v% sesame meal mixture" and "20w / v% sesame meal mixture" refer to mixtures obtained by adding and mixing 5g and 20g of sesame meal, respectively, to 100ml of a solvent such as water, a hydrophilic organic solvent, or a mixture thereof, and "5w / v% sesame meal extract" and "20w / v% sesame meal extract" refer to extracts obtained by reflux extracting the 5w / v% sesame meal mixture and the 20w / v% sesame meal mixture.

[0033] One embodiment of the present invention relates to a method for preparing a sesame seed meal extract, and more specifically, the method comprises the steps of: obtaining sesame seed meal remaining after low-temperature drying of sesame seeds and low-temperature pressing at 20°C to 45°C to extract oil; obtaining a mixture by adding and mixing the sesame seed meal to a solvent selected from water, a hydrophilic organic solvent, or a mixture thereof; and obtaining a sesame seed meal extract by performing the reflux extraction method.

[0034] In the present invention, sesame meal can be obtained from the residue remaining after extracting oil by drying sesame seeds at approximately 120°C to 140°C and then cold-pressing them at 20°C to 45°C. At this time, the drying temperature is most preferably 120°C.

[0035] The sesame meal obtained by the above low-temperature heating and low-temperature pressing is added to and mixed with a solvent selected from water, a hydrophilic organic solvent, or a mixture thereof to prepare a mixture.

[0036] The above hydrophilic organic solvent includes alcohols such as methanol and ethanol, and ethanol is particularly preferred. The above hydrophilic organic solvent may be used alone or in combination of two or more, and the concentration of the above hydrophilic organic solvent may be 30 to 70%, and preferably 50%.

[0037] The mixture prepared therefrom may contain sesame meal at a concentration of 1 to 20 w / v%, preferably at a concentration of 5 to 20 w / v%. The concentration range of 1 to 20 w / v% contains a significant amount of sesamin and sesamolin, thereby providing antioxidant, whitening, and anti-inflammatory effects. A sesame meal extract may be prepared by extracting the mixture containing dissolved sesame meal for 1 to 8 hours in a reflux cooling device set to 40°C to 100°C. The temperature during reflux extraction is 40°C to 100°C, preferably 60°C to 80°C. The reflux extraction temperature may be 1 to 8 hours, preferably 2 to 6 hours.

[0038] Another embodiment of the present invention provides a sesame seed meal extract prepared according to the method described above.

[0039] The above sesame meal extract contains sesamin and sesamolin as active ingredients, which are in an amount similar to that of sesame extract with high antioxidant activity.

[0040] Another embodiment of the present invention provides a cosmetic composition comprising the sesame seed meal extract. The cosmetic composition has antioxidant, whitening, anti-inflammatory, and regenerative effects.

[0042] The present invention will be explained in more detail below through examples.

[0043] The present invention will be explained in more detail below through the following embodiments, but the following embodiments are intended to illustrate the invention and do not limit the scope of the invention.

[0044] Furthermore, the embodiments of the present invention described below can be implemented by various substitutions, modifications, and changes within the scope of the technical concept of the present invention, and such implementation can be easily realized by a person skilled in the art to which the present invention belongs based on the description of the embodiments described above.

[0047] <Example> Preparation of Sesame Seed Meal Extract

[0048] Preparation Example 1

[0049] After washing 1 kg of sesame seeds, they were dried at 120°C for 1 hour and then pressed at 40°C to obtain the remaining sesame meal. 100 ml of 50% fermented alcohol was added to 20 g of the obtained sesame meal, and each sample was extracted once for 4 hours in a reflux condenser set to 80°C. In the same manner as above, 30% and 70% fermented alcohol were added and extraction was carried out using a reflux condenser.

[0051] Comparative Manufacturing Examples 1 and 2

[0052] 0.1N-HCl (pH 2) was added to 20g of the sesame meal obtained above, and then extracted for 3 hours using an ultrasonic device (VCX750, 20kHz, Sonic and Material Inc.) under conditions of 20–40kHz, 25–35℃, and 5–15 minutes. The extracted solution was then neutralized (pH 7.0) with 1N NaOH. The solution was dialysised for 24 hours using a dialysis membrane (Spectra / por membrane tubing, MW cutoff 14,000-16,000, Cellu sep. Membrane Filtration Products, Inc.) and filtered. Afterward, the dialysis solution was freeze-dried to prepare the ultrasonic extract.

[0054] <Example 2> Measurement of the content of active ingredients (sesamin, sesamolin) in sesame meal extract

[0055] The active ingredient content of the sesame meal extract obtained in Example 1 was measured by high-performance liquid chromatography (HPLC), and the results are listed in Table 1.

[0057] Active ingredient content (mg / L) Active ingredient 30% fermented alcohol 50% fermented alcohol 70% fermented alcohol Reflux extraction (Preparation Example 1) Sesamin 25.7 76.7 86.4 Sesamolin 4.8 34.2 40.6 Ultrasonic extraction (30 min) (Comparative Preparation Example 1) Sesamin 4.8 20.0 36.9 Sesamolin 0.0 4.8 13.2 Ultrasonic extraction (60 min) (Comparative Preparation Example 2) Sesamin 5.9 27.9 49.6 Sesamolin 0.0 7.7 19.0

[0058] As described in Table 1 above, in the case of Preparation Example 1 extracted by the reflux extraction method, the content of the active ingredient was high, and in particular, the content of the active ingredients sesamin and sesamolin in 50% fermented alcohol was very high at 76.7 mg / L and 34.2 mg / L, respectively.

[0060] <Example 3> Setting the Concentration of Sesame Seed Meal Extract

[0061] To determine the concentration of the sesame meal extract of the present invention, the extract obtained in Example 1 was reflux-extracted by mixing 100 ml of 50% ethanol with 5 g, 10 g, 15 g, and 20 g of sesame meal, respectively, as described in Examples 1 to 4 of Table 1, to obtain the extract.

[0062] It was decided to set the concentration of a sesame meal extract having an active ingredient content similar to that of a 5 w / v% sesame extract with high antioxidant activity (100 ml of 50% fermented alcohol per 5 g of sesame).

[0063] As a result, it was confirmed that the sesamin and sesamolin content in the 20w / v% sesame meal composition (100ml of 50% fermented alcohol per 20g of sesame meal) was 164.5mg / L and 61.9mg / L, respectively, and a small but significant amount of sesamin and sesamolin content was also confirmed in the 5w / v% sesame meal composition (100ml of 50% fermented alcohol per 5g of sesame meal).

[0065] Experimental Example 1: Evaluation of Antioxidant Efficacy

[0066] (1) DPPH radical scavenging activity

[0067] The sesame meal extract of the present invention, prepared according to Example 1 above, was diluted to different concentrations and mixed in equal amounts with DPPH (0.2 mM ethanol solution) (Sigma, USA). After reacting at room temperature for 30 minutes, the absorbance was measured at a wavelength of 520 nm. Ascorbic acid was used as a control to compare the relative antioxidant effects. The antioxidant effect was expressed as 50% DPPH radical scavenging activity.

[0068] DPPH radical scavenging activity (%) was calculated using the formula (1 - absorbance of sample-added group / absorbance of sample-free group). The SC50 value represents the minimum concentration required to scavenge 50% of the generated radicals in % concentration units, and the results are shown in Table 2 below.

[0070] Analysis sample 50% alcohol extract SC 50 control group Ascorbic acid 6㎍ / ㎖ Comparative Example 1 Sesame 5w / v% 20% Comparative Example 2 Roasted Sesame 5w / v% 26% Example 1 Sesame meal 5w / v% 12% Example 2 Sesame meal 10w / v% 10% Example 3 Sesame meal 15w / v% 8% Example 4 Sesame meal 20w / v% 4%

[0071] As shown in Table 2 above, the SC50 values ​​of the control ascorbic acid and Example 4 (sesame meal 20 w / v% extract) were confirmed to be 6 μg / ml and 4%, respectively. In this case, the % concentration means that 4% of the 20% sesame meal extract is required to scavenge 50% of radicals, and a lower concentration % indicates a higher DPPH radical scavenging ability of the sesame meal composition of the present invention. As shown in Table 2, Examples 1 to 4 showed a higher antioxidant effect compared to Comparative Examples 1 and 2.

[0073] Experimental Example 2: Evaluation of Whitening Efficacy

[0074] (1) Inhibitory ability of melanin synthesis (cell experiment)

[0075] B16F10 melanogenic cells were seeded into each well of a 6-well plate at a rate of 1 x 10⁵ cells / well (final volume 3 ml) and cultured in a 37°C, 5% CO₂ incubator for 24 hours. Afterward, the medium was removed from each well and replaced with fresh DMEM (10% FBS). Sesame meal extract samples were prepared at different concentrations and pretreated in each well for 30 minutes.

[0076] Afterward, 1 ppm of α-MSH was added and cultured for 48 hours. Then, the culture medium was removed, 1 ml of PBS was added to each well, and cells were detached by scraping each well with a cell scraper (Corning, USA) and transferred to a new 1.5 ml tube. Next, the sample was centrifuged at 13,000 rpm at 4°C for 3 minutes to remove the supernatant, and the cell pellet was recovered. 200 ml of lysis buffer (1% Triton X-100, 1 mM PMSF, 50 mM Tris-HCl (pH 7.0), 2 mM EDTA (pH 8.0), 150 mM NaCl, 4°C) was added. After lysing the cells by vortexing at 10-minute intervals for 30 minutes, the sample was centrifuged at 13,000 rpm at 4°C for 30 minutes to remove the supernatant, and the melanin-containing cell pellet was recovered and washed once with 100 ml of an Ether / Isopropanol 1:1 solution. The sample was centrifuged at 13,000 rpm for 10 minutes to remove the supernatant, dried at 60°C for approximately 3 hours, and then 2N NaOH containing 10% DMSO 150 ml was added to each well and intracellular melanin was lysed at 60°C for 1 hour. 100 ml of the supernatant obtained from the lysis was added to each well of a 96-well plate, and the absorbance was measured at 405 nm using an ELISA reader. As a negative control, an amount of DMSO, the solvent in which the sample was dissolved, was added in the same quantity as the sample treatment, instead of the sample. As a positive control, only Rucinol was treated without the sample to maximize melanin production. The melanin synthesis inhibition rate (%) was calculated using the following Equation 1.

[0077] [Mathematical Formula 1]

[0078] Melanin synthesis inhibition rate (%) = [(AC) / (AB)] x 100

[0079] A. Amount of melanin in the positive control group

[0080] B. Amount of melanin in the negative control group

[0081] C. Amount of melanin in the test substance

[0082] Table 3 shows the results of measuring the melanin synthesis inhibition rate (%), and Figure 1 shows the results of measuring the melanin synthesis rate (%).

[0084] Rucinol (150μM) Dilution concentration of sesame meal extract 25㎍ / ㎖ 50㎍ / ㎖ 100㎍ / ㎖ control group 91±1.40 - - - Example 1 (5w / v% sesame meal extract) - 11±14.18 69±2.27 71±2.13 Example 4 (20w / v% sesame meal extract) - 20±9.19 62±3.79 71±0.24

[0086] (2) Evaluation of tyrosinase activity inhibitory ability

[0087] B16F10 melanocytes (or HEMn-LP melanocytes) were placed in each well of a 6-well plate at a rate of 1 x 10⁵ cells / well (Final volume 3 ml) and cultured for 24 hours in a 37°C, 5 wt% CO₂ incubator. Afterward, the medium was removed from each well and replaced with fresh DMEM. Sesame meal extract samples (100 ml of fermented alcohol per 5 g and 20 g of sesame meal) were prepared at different concentrations and pretreated in each well for 30 minutes.

[0088] Next, 1 ppm α-MSH was added and cultured for 48 hours. Afterward, the culture medium was removed, 1 ml of PBS was added to each well, and cells were detached by scraping each well with a cell scraper and transferred to individual 1.5 ml tubes. Subsequently, the cells were centrifuged at 4°C and 13,000 rpm for 3 minutes to remove the supernatant, and the cell pellet was recovered. 200 ml of lysis buffer (100 mM sodium phosphate buffer (pH 6.8) containing 1% Triton X-100 and 1 mM PMSF) was added. The cells were lysed by vortexing at 10-minute intervals for 30 minutes, followed by centrifugation at 4°C and 13,000 rpm for 30 minutes, and the supernatant was transferred to new 1.5 ml tubes. Total protein was quantified using a BCA protein assay kit (PIERCE, USA). 40 ml of the quantified sample was placed into each well of a 96-well plate, followed by the addition of 160 ml of L-3,4-dihydroxyphenylalanine (L-DOPA), which was dissolved in phosphate buffer (pH 6.8) immediately prior to the experiment at a concentration of 2 mg / ml. After incubation at 37°C for 20 to 60 minutes, absorbance was measured at 475 nm using an ELISA reader. As a negative control, an amount of DMSO—the solvent in which the sample was dissolved—was added in place of the sample, equal to the sample treatment amount. As a positive control, only Rucinol was added without any sample treatment to maximize tyrosinase enzyme activity. The tyrosinase enzyme activity inhibition rate (%) was calculated using the following Equation 2.

[0089] [Mathematical Formula 2]

[0090] Tyrosinase activity inhibition rate (%) = [(AC) / (AB)] x 100

[0091] A. Tyrosinase enzyme activity rate of the positive control

[0092] B. Tyrosinase enzyme activity rate of the negative control group

[0093] C. Tyrosinase enzyme activity of the test substance

[0094] Table 4 shows the results of measuring the tyrosinase activity inhibition rate (%) of the samples, and Figure 2 shows the results of measuring the tyrosinase activity rate (%) of the samples.

[0096] Rucinol (150μM) Dilution concentration of sesame meal extract 25㎍ / ㎖ 50㎍ / ㎖ 100㎍ / ㎖ control group 94±1.37 - - - Example 1 (5w / v% sesame meal extract) - 30±1.83 43±3.87 57±4.48 Example 4 (20w / v% sesame meal extract) - 37±6.88 51±4.04 59±5.57

[0097] According to Table 4 above, the whitening efficacy was confirmed by showing a tyrosinase activity inhibitory ability of 59% at a concentration of 100 μg / ml of 20 w / v% sesame meal extract.

[0099] Experimental Example 3: Anti-inflammatory Evaluation

[0100] (1) PGE2 expression inhibition ability (cell experiment)

[0101] PGE2 (prostaglandin E2) is a substance that plays an important role in inducing inflammation, and it was confirmed that the sesame meal extracts of Examples 1 and 4 inhibit PGE2 expression.

[0102] Human keratinocytes were adjusted to 5 x 10⁵ cells / well using DMEM medium supplemented with 10% FBS and inoculated into a 6-well plate. The sesame meal extract of the present invention was treated at different concentrations, and after 30 minutes of incubation, LPS (Lipopolysaccharide, 1 μg / ml) was added and incubated for 18 hours. Dexamethasone was used as a positive control. The amount of PGE2 produced was measured using a PGE2 ELISA kit (R&D systems, Inc, USA). The PGE2 expression inhibition rate (%) was measured based on the amount of PGE2 produced in the examples or the negative control group not treated with PDTC, and the results are described in Table 5 and Figure 3.

[0103] According to Table 5 above, Example 1 (5 w / v% sesame meal extract) showed a PGE2 expression inhibition ability of 37%, and Example 4 (20 w / v% sesame meal extract) showed a PGE2 expression inhibition ability of 26%, confirming the anti-inflammatory efficacy.

[0105] Dexamethasone (150μM) Dilution concentration of sesame meal extract 25 ㎍ / ㎖ 50 ㎍ / ㎖ 100 ㎍ / ㎖ control group 48±11.88 - - - Example 1 (5w / v% sesame meal extract) - 29±1.70 32±5.54 37±6.71 Example 4 (20w / v% sesame meal extract) - 16±0.70 25±3.14 26±4.17

[0107] Experimental Example 4: Evaluation of Regenerative Efficacy

[0108] Keratinocytes seeded into a 1.5 x 10⁵ cells / 12-well plate were cultured in DMEM medium containing 10% (v / v) FBS for 24 hours, after which the medium was replaced with a medium without FBS, and "scratching-damage" was induced in each well using the tip of a P200 pipette. TNF-alpha, which inhibits the regeneration and repair of the cell layer, was applied to the "scratching-damaged" HaCaT cell layer. TGF-beta was used as a positive control, and it was confirmed that cell damage was repaired even when repair was blocked by TNF-alpha when TGF-beta was used. HaCaT cells treated with TNF-alpha were then treated with sesame meal extract (100 ml of fermented alcohol per 5 g and 20 g of sesame meal) and cultured for an additional 20 hours at 37°C and 5% CO₂. After additional culture, the medium was removed and images were taken with an optical microscope (Digital inverted microscope, AME-12111; Advanced Microscopy Group, USA)

[0110] number Analysis sample Comparative example TNF-alpha Comparative example TNF-alpha + TGF-beta Example 1 5w / v% sesame meal extract Example 4 20w / v% sesame meal extract

[0111] The results were shown in Figure 4. It was confirmed that the 20 w / v% sesame meal extract had an excellent regenerative effect, equivalent to or greater than that of the positive control, TGF-beta.

[0113] Experimental Example 5: Preparation of Essence

[0114] An essence containing the ingredients of Table 7 below was prepared.

[0116] INCI name Content (%) water 79.83 Disodium EDTA 0.02 1,2-hexanediol 2.00 Dipropylene glycol 4.00 Capryl glycol 0.20 glycerin 1.80 Butylene glycol 6.50 Hydroxyethylcellulose 0.02 Xanthan sword 0.20 Carbomer 0.20 Tromethamine 0.15 Sesame meal 20 w / v% extract 5.00 spices 0.08 total 100.00

[0117] Twenty-three male and female subjects aged 24 to 55 (44.1 ± 8.7 years) (1 male, 22 females) were instructed to apply an appropriate amount of the above essence evenly to their entire face twice a day, in the morning and evening. Statistical analysis was performed using GraphPad InStat 3 software to test for changes before and after use via Repeated measures ANOVA at a significance level of p<0.05.

[0119] (1) Check for melanin improvement

[0120] Melanin in both cheek areas was measured using Antera3D at points before, 2 weeks after, and 4 weeks after the use of the test product. As a result, it was confirmed that there was a statistically significant decrease (p<0.05, transient, difference) at the point 4 weeks after use compared to before use. The experimental results are listed in Table 8, and an image of an example is shown in Figure 5.

[0122] NO. melanin Before use After 2 weeks of use After 4 weeks of use region right left right left right left 1 0.539 0.540 0.533 0.538 0.545 0.549 2 0.552 0.549 0.518 0.526 0.501 0.515 3 0.658 0.666 0.647 0.664 0.641 0.661 4 0.683 0.719 0.672 0.712 0.663 0.703 5 0.606 0.620 0.631 0.628 0.615 0.604 6 0.516 0.536 0.507 0.534 0.504 0.528 7 0.600 0.634 0.605 0.617 0.583 0.612 8 0.630 0.647 0.645 0.649 0.601 0.619 9 0.738 0.732 0.730 0.738 0.732 0.721 10 0.709 0.697 0.712 0.702 0.724 0.705 11 0.596 0.638 0.603 0.640 0.607 0.654 12 0.588 0.599 0.572 0.566 0.588 0.597 13 0.608 0.605 0.611 0.620 0.609 0.599 14 0.631 0.662 0.653 0.677 0.659 0.682 15 0.566 0.574 0.549 0.574 0.553 0.570 16 0.558 0.624 0.532 0.575 0.538 0.579 17 0.521 0.557 0.528 0.556 0.518 0.547 18 0.571 0.605 0.570 0.604 0.579 0.608 19 0.557 0.573 0.557 0.584 0.532 0.568 20 0.520 0.562 0.519 0.565 0.510 0.545 21 0.630 0.649 0.603 0.642 0.598 0.633 22 0.512 0.526 0.524 0.541 0.535 0.535 23 0.628 0.638 0.620 0.649 0.595 0.602 Evaluation time Case Average (au) Standard deviation (SD) Improvement rate (%) p -value Before use 46 0.606 0.060 - - After 2 weeks of use 46 0.603 0.062 0.46 ns, p >0.05 After 4 weeks of use 46 0.597 0.062 0.99 *, p <0.05

[0123] The improvement rate is based on the time before use. ns: Not significant, *: Significant difference from before

[0125] (2) Improve skin moisture

[0126] Melanin in both cheek areas was measured using Skin-O-Mat at points before, 2 weeks, and 4 weeks after the use of the test product. As a result, it was confirmed that there was a statistically significant increase at the 4-week mark compared to before use (p<0.01, transient, difference). The experimental results are listed in Table 9.

[0128] NO. moisture Before use After 2 weeks of use After 4 weeks of use region right left right left right left 1 59.53 60.67 54.17 59.37 62.53 62.67 2 61.33 62.57 59.90 59.20 59.37 59.63 3 61.03 60.27 56.10 56.87 65.50 66.30 4 62.40 63.33 52.53 63.37 62.93 64.60 5 66.07 63.23 62.73 64.43 69.27 72.67 6 64.73 68.27 64.50 72.03 65.67 69.33 7 63.77 60.40 55.17 55.33 71.57 60.83 8 68.17 59.53 60.23 63.23 61.73 65.17 9 66.93 78.80 68.93 74.60 77.03 78.67 10 57.33 64.93 63.47 66.97 62.37 65.40 11 64.27 63.60 61.17 60.83 72.03 72.53 12 64.43 65.00 63.80 72.23 70.37 74.60 13 70.30 70.83 70.83 70.17 77.10 78.13 14 62.77 68.53 61.40 71.00 65.83 72.33 15 54.87 56.97 57.13 58.80 54.70 59.20 16 50.23 52.43 58.67 63.57 64.10 69.47 17 56.60 55.97 55.23 57.60 63.80 69.80 18 67.67 65.17 63.30 72.00 68.40 74.63 19 62.87 62.03 60.23 64.57 64.47 68.53 20 62.47 61.27 60.90 59.47 58.93 62.13 21 53.87 62.53 60.27 67.33 68.77 71.50 22 60.30 53.83 62.83 66.60 57.97 64.03 23 50.67 54.90 60.10 68.27 63.03 66.10 Evaluation time Case Average (ug / cm²) 2 ) Standard deviation (SD) Improvement rate (%) p -value Before use 46 61.91 3.28 - - After 2 weeks of use 46 62.64 4.99 1.19 ns, p >0.05 After 4 weeks of use 46 66.86 5.44 8.01 **, p <0.01

[0129] The improvement rate is based on the time before use. ns: Not significant, **: Significant difference from before

Claims

Claim 1 delete Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 A cosmetic composition having antioxidant, whitening, anti-inflammatory, and regenerative effects, comprising a sesame seed extract prepared according to a method comprising the steps of: drying sesame seeds at 120°C to 140°C, then cold-pressing at 20°C to 45°C to obtain the remaining sesame seed seed meal; adding and mixing the sesame seed meal to a hydrophilic organic solvent at a concentration of 50 volume% to obtain a mixture containing 20 w / v% of sesame seed meal; and performing a reflux extraction method in which the mixture is reflux-extracted for 2 to 6 hours in a reflux cooling device set at 60°C to 80°C to obtain a sesame seed meal extract, wherein the sesame seed meal extract containing sesamin and sesamolin is provided through the reflux extraction, and the same sesame seed meal extract exhibits antioxidant, whitening, anti-inflammatory, and regenerative effects, wherein the hydrophilic organic solvent comprises methanol and ethanol.

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