Composition for preventing hair loss and promoting hair growth

Abstract

The present invention provides a composition for preventing hair loss and promoting hair growth comprising awapuhi extract, caffeine, and macadamia seed oil.

Description

Technology Field

[0001] The present invention relates to a composition for preventing hair loss and promoting hair growth comprising awapuhi extract, caffeine, and macadamia seed oil. Background Technology

[0002] Generally, human hair protects the skull from external physical impacts, ultraviolet rays, fine dust, and sudden temperature changes, and plays a role in absorbing heavy metals that are unnecessary to the body, such as arsenic (As), mercury (Hg), or zinc (Zn), and expelling them from the body.

[0003] Furthermore, the human scalp is composed of the epidermis, dermis, and subcutaneous tissue; among these, the epidermis, located on the outermost layer, functions to protect the body's sensitive tissues from damage.

[0004] The hair cycle can be divided into the growth phase (anagen), during which hair grows; the regression phase (catagen), during which growth ends and the hair bulb shrinks; the resting phase (telogen), during which the hair papilla ceases activity and keeps the hair on the scalp; and the active phase, during which the hair papilla begins activity or generates new hair to shed old hair. This hair cycle varies by individual and gender; generally, about 15% of total hair falls out and is regenerated during the regression and resting phases. Therefore, if the percentage of hair in the resting phase exceeds 20%, it is considered a state of significant hair loss. Losing an average of 50 to 100 hairs per day is referred to as telogen effluvium, which is a physiologically normal phenomenon; however, if the rate of hair loss exceeds this level, it is necessary to suspect male pattern baldness or alopecia areata.

[0005] In particular, while there was a widespread perception that hair loss was hereditary, recent research has consistently shown that hair loss is a phenomenon of aging and that its onset can be accelerated by changes in the living environment.

[0006] In line with this trend, cosmetic compositions that prevent hair loss by promoting and improving hair growth through the artificial supply of nutrients are being developed. However, if the ingredients of the aforementioned improvement products remain on the scalp, they can cause irritation such as stinging, itching, or redness and swelling; consequently, consumers with sensitive scalps are reluctant to use hair loss prevention compositions.

[0007] Therefore, there is an urgent need for scalp products that prevent hair loss and promote hair growth, which provide gentle application to the hair and alleviate and soothe irritation, while also offering the effect of preventing and stopping hair loss by promoting scalp growth. Prior art literature

[0008] Republic of Korea Registered Patent No. 10-2232873 The problem to be solved

[0009] The objective of the present invention is to provide a composition for preventing hair loss and promoting hair growth.

[0010] The problems of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0011] To achieve the above objective, the present invention

[0012] A composition for preventing hair loss and promoting hair growth is provided, comprising awapuhi extract, caffeine, and macadamia seed oil.

[0013] In addition, the above composition comprises awapuhi extract, caffeine, macadamia seed oil, olive oil, avocado oil, sunflower seed oil, soybean oil, annatto seed oil, argan kernel oil, drumstick seed oil, marula seed oil, camellia seed oil, pistachio seed oil, cucui seed oil, grape seed oil, cyclopentasiloxane, isopropyl myristate, dimethicone, phenyl trimethicone, isopropyl palmitate, hydrolyzed keratin, hydrolyzed collagen, hydrolyzed silk, hydrolyzed elastin, hydrolyzed soy protein, hydrolyzed corn protein, leucine, alanine, lysine, arginine, tyrosine, phenylalanine, threonine, proline, valine, isoleucine, histidine, methionine, It includes glycine, serine, cysteine, caprylyl glycol, glutamic acid, aspartic acid, butylene glycol, ethylhexylglycerin, propanediol, tocopherol, and 1,2-hexanediol.

[0014] In addition, the above composition comprises 0.1-10 parts by weight of awapuhi extract, 0.1-10 parts by weight of caffeine, 0.1-10 parts by weight of macadamia seed oil, 0.1-10 parts by weight of olive oil, 0.1-10 parts by weight of avocado oil, 0.1-10 parts by weight of sunflower seed oil, 0.1-10 parts by weight of soybean oil, 0.1-10 parts by weight of annatto seed oil, 0.1-10 parts by weight of argan kernel oil, 0.1-10 parts by weight of drumstick seed oil, 0.1-10 parts by weight of marula seed oil, 0.1-10 parts by weight of camellia seed oil, 0.1-10 parts by weight of pistachio seed oil, 0.1-10 parts by weight of cucui seed oil, 0.1-10 parts by weight of grape seed oil, and cyclopentasiloxane. 0.1-5.0 parts by weight, isopropyl myristate 0.1-5.0 parts by weight, dimethicone 0.1-5.0 parts by weight, phenyl trimethicone 0.1-5.0 parts by weight, isopropyl palmitate 0.1-5.0 parts by weight, hydrolyzed keratin 0.1-5.0 parts by weight, hydrolyzed collagen 0.1-5.0 parts by weight, hydrolyzed silk 0.1-5.0 parts by weight, hydrolyzed elastin 0.1-5.0 parts by weight, hydrolyzed soy protein 0.1-5.0 parts by weight, hydrolyzed corn protein 0.1-5.0 parts by weight, leucine 0.3-0.7 parts by weight, alanine 0.1-0.5 parts by weight, lysine 0.1-0.5 parts by weight, arginine 0.1-0.5 parts by weight, tyrosine 0.1-0.5 parts by weight, phenylalanine 0.1-0.5 parts by weight, threonine 0.1-0.5 parts by weight, proline 0.1-0.5 parts by weight, valine 0.1-0.5 parts by weight, isoleucine 0.1-0.5 parts by weight, histidine 0.1-0.5 parts by weight, methionine 0.1-0.5 parts by weight, glycine 0.1-0.5 parts by weight, serine 0.1-0.5 parts by weight, cysteine ​​0.1-0.5 parts by weight, caprylyl glycol 0.08-0.12 parts by weight, glutamic acid 0.08-0.12 parts by weight, aspartic acid 0.08-0.12 parts by weight, butylene glycol 1-3 parts by weight, ethylhexylglycerin 0.08-0.12 parts by weight, propanediol 0.08-0.12 parts by weight, tocopherol 0.08-0.12 parts by weight and 1,2-hexanediol 0.It contains 0.8-0.12 parts by weight.

[0015] In addition, the above composition further comprises 0.1 to 10 parts by weight of a functional extract, and

[0016] The above functional extract is characterized by being an extract obtained from a complex of black cumin seeds, Job's tears seeds, and quinoa mixed in a weight ratio of 1:1:1.

[0017] In addition, the above composition further comprises 0.1 to 10 parts by weight of a functional extract, and

[0018] The above functional extract is,

[0019] A step of washing black cumin seeds, Job's tears seeds, and quinoa, and heat-treating them at a temperature of 70-80°C for 20-40 minutes; a step of steaming the heat-treated black cumin seeds, Job's tears seeds, and quinoa at a pressure of 0.095-0.105 MPa and a temperature of 140-160°C for 80-100 minutes; a step of hot-air drying the steamed black cumin seeds, Job's tears seeds, and quinoa at a temperature of 58-62°C for 5-7 hours; The method is characterized by being prepared by performing the following steps: mixing 48-52 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa mixed in a weight ratio of 1:1:1 with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a Bacillus coagulans strain, and fermenting at a temperature of 30-36°C for 2-4 days; after fermentation, adding 33-37 parts by weight of hexane and extracting at a temperature of 90-110°C for 8-10 hours to prepare an extract; and concentrating the extract under reduced pressure and freeze-drying it.

[0020] In addition, the above composition further comprises 0.1 to 10 parts by weight of a functional extract, and

[0021] The above functional extract is,

[0022] A step of washing black cumin seeds, Job's tears seeds, and quinoa, and heat-treating them at a temperature of 70-80°C for 20-40 minutes; a step of steaming the heat-treated black cumin seeds, Job's tears seeds, and quinoa at a pressure of 0.095-0.105 MPa and a temperature of 140-160°C for 80-100 minutes; a step of hot-air drying the steamed black cumin seeds, Job's tears seeds, and quinoa at a temperature of 58-62°C for 5-7 hours; a step of mixing 78-82 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa in a weight ratio of 1:1:1 with 18-22 parts by weight of mycelial powder, and fermenting and aging the mixture at a temperature of 25-35°C for 2-4 days; A step of obtaining a fermented and aged product after fermentation and aging, mixing 48-52 parts by weight of the obtained fermented and aged product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a Bacillus coagulans strain, and fermenting at a temperature of 30-36℃ for 2-4 days; a step of preparing an extract by adding 33-37 parts by weight of hexane after fermentation and extracting at a temperature of 90-110℃ for 8-10 hours; and a step of concentrating the extract under reduced pressure and freeze-drying; thereby being prepared.

[0023] The above mycelial powder is characterized by being prepared by washing oyster mushrooms, poison ivy, and wheat bran, drying them at a temperature of 48-52℃ for 8-10 hours, and grinding them.

[0024] In addition, the present invention

[0025] Awapuhi extract 0.1-10 parts by weight, caffeine 0.1-10 parts by weight, macadamia seed oil 0.1-10 parts by weight, olive oil 0.1-10 parts by weight, avocado oil 0.1-10 parts by weight, sunflower seed oil 0.1-10 parts by weight, soybean oil 0.1-10 parts by weight, annatto seed oil 0.1-10 parts by weight, argan kernel oil 0.1-10 parts by weight, drumstick seed oil 0.1-10 parts by weight, marula seed oil 0.1-10 parts by weight, camellia seed oil 0.1-10 parts by weight, pistachio seed oil 0.1-10 parts by weight, cucui seed oil 0.1-10 parts by weight, grape seed oil 0.1-10 parts by weight, cyclopentasiloxane 0.1-5.0 parts by weight, isopropyl myristate 0.1-5.0 parts by weight, dimethicone 0.1-5.0 parts by weight, phenyl trimethicone 0.1-5.0 parts by weight, isopropyl palmitate 0.1-5.0 parts by weight, hydrolyzed keratin 0.1-5.0 parts by weight, hydrolyzed collagen 0.1-5.0 parts by weight, hydrolyzed silk 0.1-5.0 parts by weight, hydrolyzed elastin 0.1-5.0 parts by weight, hydrolyzed soy protein 0.1-5.0 parts by weight, hydrolyzed corn protein 0.1-5.0 parts by weight, leucine 0.3-0.7 parts by weight, alanine 0.1-0.5 parts by weight, lysine 0.1-0.5 parts by weight, arginine 0.1-0.5 parts by weight, tyrosine 0.1-0.5 parts by weight, phenylalanine 0.1-0.5 parts by weight, threonine 0.1-0.5 parts by weight, proline 0.1-0.5 parts by weight, valine 0.1-0.5 parts by weight, isoleucine 0.1-0.5 parts by weight, histidine 0.1-0.5 parts by weight, methionine 0.1-0.5 parts by weight, glycine 0.1-0.5 parts by weight, serine 0.1-0.5 parts by weight, cysteine ​​0.1-0.5 parts by weight, caprylyl glycol 0.08-0.12 parts by weight, glutamic acid 0.08-0.12 parts by weight, aspartic acid 0.08-0.12 parts by weight, butylene glycol 1-3 parts by weight, ethylhexylglycerin 0.08-0.12 parts by weight, propanediol 0.08-0.12 parts by weight, tocopherol 0.08-0.12 parts by weight and 1,2-hexanediol 0.08-0.12 parts by weight.A method for preparing a composition for preventing hair loss and promoting hair growth is provided, comprising the step of mixing 12 parts by weight. Effects of the invention

[0026] The composition according to the present invention is effective in preventing hair loss and promoting hair growth. Specific details for implementing the invention

[0027] Various embodiments are described in more detail below. The embodiments described herein may be modified in various ways. Specific embodiments may be described in detail in the detailed description. However, the specific embodiments disclosed are intended only to facilitate understanding of various embodiments. Accordingly, the technical concept is not limited by the specific embodiments disclosed, and it should be understood that it includes all equivalents or substitutions that fall within the spirit and scope of the invention.

[0028] Terms including ordinal numbers, such as first, second, first, second, etc., may be used to describe various components, but these components are not limited by the aforementioned terms. The aforementioned terms are used solely for the purpose of distinguishing one component from another.

[0029] In this specification, terms such as "comprising" or "having" are intended to indicate the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof. When a component is described as being "connected" or "connected" to another component, it should be understood that it may be directly connected to or connected to that other component, or that there may be other components in between. On the other hand, when a component is described as being "directly connected" or "directly connected" to another component, it should be understood that there are no other components in between.

[0030] Furthermore, in describing the present invention, if it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the essence of the invention, such detailed description is abbreviated or omitted.

[0031] The present invention

[0032] A composition for preventing hair loss and promoting hair growth is provided, comprising awapuhi extract, caffeine, and macadamia seed oil.

[0033] Hereinafter, a composition for preventing hair loss and promoting hair growth according to the present invention will be described in detail.

[0035] The above composition comprises awapuhi extract, caffeine, macadamia seed oil, olive oil, avocado oil, sunflower seed oil, soybean oil, annatto seed oil, argan kernel oil, drumstick seed oil, marula seed oil, camellia seed oil, pistachio seed oil, cucui seed oil, grape seed oil, cyclopentasiloxane, isopropyl myristate, dimethicone, phenyl trimethicone, isopropyl palmitate, hydrolyzed keratin, hydrolyzed collagen, hydrolyzed silk, hydrolyzed elastin, hydrolyzed soy protein, hydrolyzed corn protein, leucine, alanine, lysine, arginine, tyrosine, phenylalanine, threonine, proline, valine, isoleucine, histidine, methionine, It may include glycine, serine, cysteine, caprylyl glycol, glutamic acid, aspartic acid, butylene glycol, ethylhexylglycerin, propanediol, tocopherol, and 1,2-hexanediol.

[0036] In addition, the above composition comprises 0.1-10 parts by weight of awapuhi extract, 0.1-10 parts by weight of caffeine, 0.1-10 parts by weight of macadamia seed oil, 0.1-10 parts by weight of olive oil, 0.1-10 parts by weight of avocado oil, 0.1-10 parts by weight of sunflower seed oil, 0.1-10 parts by weight of soybean oil, 0.1-10 parts by weight of annatto seed oil, 0.1-10 parts by weight of argan kernel oil, 0.1-10 parts by weight of drumstick seed oil, 0.1-10 parts by weight of marula seed oil, 0.1-10 parts by weight of camellia seed oil, 0.1-10 parts by weight of pistachio seed oil, 0.1-10 parts by weight of cucui seed oil, 0.1-10 parts by weight of grape seed oil, and cyclopentasiloxane. 0.1-5.0 parts by weight, isopropyl myristate 0.1-5.0 parts by weight, dimethicone 0.1-5.0 parts by weight, phenyl trimethicone 0.1-5.0 parts by weight, isopropyl palmitate 0.1-5.0 parts by weight, hydrolyzed keratin 0.1-5.0 parts by weight, hydrolyzed collagen 0.1-5.0 parts by weight, hydrolyzed silk 0.1-5.0 parts by weight, hydrolyzed elastin 0.1-5.0 parts by weight, hydrolyzed soy protein 0.1-5.0 parts by weight, hydrolyzed corn protein 0.1-5.0 parts by weight, leucine 0.3-0.7 parts by weight, alanine 0.1-0.5 parts by weight, lysine 0.1-0.5 parts by weight, arginine 0.1-0.5 parts by weight, tyrosine 0.1-0.5 parts by weight, phenylalanine 0.1-0.5 parts by weight, threonine 0.1-0.5 parts by weight, proline 0.1-0.5 parts by weight, valine 0.1-0.5 parts by weight, isoleucine 0.1-0.5 parts by weight, histidine 0.1-0.5 parts by weight, methionine 0.1-0.5 parts by weight, glycine 0.1-0.5 parts by weight, serine 0.1-0.5 parts by weight, cysteine ​​0.1-0.5 parts by weight, caprylyl glycol 0.08-0.12 parts by weight, glutamic acid 0.08-0.12 parts by weight, aspartic acid 0.08-0.12 parts by weight, butylene glycol 1-3 parts by weight, ethylhexylglycerin 0.08-0.12 parts by weight, propanediol 0.08-0.12 parts by weight, tocopherol 0.08-0.12 parts by weight and 1,2-hexanediol 0.It may include 0.8-0.12 parts by weight.

[0037] Awapuhi extract is obtained from the rhizome of Zingiber zerumbet and contains phytochemicals of the gingerol and shogaol series as well as polyphenols, exhibiting excellent anti-inflammatory and antioxidant effects. When applied to the scalp, it alleviates excessive sebum and micro-inflammation, maintains a clean scalp environment to protect hair follicles, and simultaneously smooths hair cuticles to provide shine and elasticity, thereby helping to prevent hair loss and maintain hair health.

[0038] Caffeine is an alkaloid derived from sources such as the seeds of *Coffea arabica*, and it contributes to enhancing hair follicle activity by promoting cellular metabolism and increasing microcirculation. When applied to the scalp, it improves blood flow around the hair follicles to aid in the supply of nutrients to the hair roots, and by partially inhibiting the action of DHT associated with androgenetic alopecia, it can extend the hair growth phase and delay the transition to the resting phase, thereby acting beneficially in preventing hair loss and promoting hair growth.

[0039] Macadamia seed oil is obtained by cold-pressing the nuts of Macadamia integrifolia. It contains unsaturated fatty acids, primarily palmitoleic acid and oleic acid, as well as Vitamin E and polyphenols, making it excellent for strengthening the skin barrier and providing antioxidant effects. When applied to the scalp and hair, it moisturizes dry scalps, alleviates dandruff, and adds shine and softness to damaged hair, thereby contributing to the prevention of hair loss and hair improvement.

[0040] Olive oil is obtained by cold-pressing the fruit of Olea europaea. Rich in oleic acid, squalene, tocopherol, and polyphenols, it offers excellent antioxidant and skin-softening effects. When applied to the scalp, it replenishes the lipid barrier to inhibit moisture loss and alleviate oxidative stress, making it suitable for protecting hair follicles and creating an environment for healthy hair growth.

[0041] Avocado oil is obtained by pressing the fruit of the Persea gratissima. It is rich in oleic acid, linoleic acid, vitamins A, D, and E, and protein, and is known as the "butter of the forest" for its excellent nourishing and moisturizing effects. When applied to the scalp and hair, it alleviates dryness through deep hydration and nourishment, and enhances hair elasticity and shine, making it useful for scalp care related to hair loss.

[0042] Sunflower seed oil is extracted from the seeds of Helianthus annuus and contains linoleic acid, oleic acid, vitamins A and E, and lecithin, providing antioxidant, soothing, and skin barrier-forming effects. Its high oxidative stability makes it suitable as a base oil for cosmetics, and when applied to the scalp, it helps moisturize and prevent aging without causing acne.

[0043] Soybean oil is a soybean-based oil extracted from the seeds of the Glycine max plant. It contains linoleic acid, oleic acid, vitamin E, and lecithin, offering excellent skin softening and moisturizing effects. When applied to the scalp, it can contribute to maintaining hair health by regulating lipid balance and reducing oxidative stress around hair follicles through its antioxidant action.

[0044] Annatto seed oil is obtained from the seeds of Bixa orellana and contains carotenoid pigments such as bixin and norbixin, as well as unsaturated fatty acids, exhibiting powerful antioxidant and pigment-stabilizing effects. In scalp care, it protects hair follicles from UV rays and helps to even out skin tone, making it suitable for use as an auxiliary oil for hair loss prevention.

[0045] Argan kernel oil is obtained by pressing the nuts of Argania spinosa. Rich in linoleic acid, oleic acid, phytosterols, and vitamin E, it offers excellent moisturizing, antioxidant, and anti-aging effects. It absorbs lightly without clogging pores and is suitable for supporting hair growth when applied to the scalp by improving skin elasticity and alleviating inflammation.

[0046] Drumstick seed oil is extracted from the seeds of Moringa oleifera and contains behenic acid, oleic acid, vitamin E, and glucosinolates, providing powerful antioxidant, anti-inflammatory, and moisturizing effects. When applied to the scalp, it helps activate hair follicles by improving microcirculation and supplying nutrients, making it beneficial for the care of dry and inflamed scalps.

[0047] Marula seed oil is obtained from the nuts of Sclerocarya birrea and contains oleic acid, stearic acid, vitamins C and E, and epicatechin, providing excellent antioxidant and barrier-strengthening effects. Its light and fast absorption makes it suitable for oily scalps, and it contributes to preventing hair loss through hair protection and moisturization.

[0048] Camellia seed oil is extracted from the seeds of Camellia japonica or Camellia sinensis. It is rich in oleic acid, linoleic acid, and vitamin E, providing excellent moisturizing and shine-enhancing effects. When applied to the scalp and hair, it supplies moisture and nourishment while adding a radiant shine, and is effective in relieving inner dryness and improving hair health.

[0049] Pistachio seed oil is obtained from the nuts of Pistacia vera and contains oleic acid, linoleic acid, and antioxidant polyphenols, providing skin softening and protective effects. In scalp care, it helps support hair growth by stabilizing the hair follicle environment through antioxidant and moisturizing properties.

[0050] Cucui seed oil is extracted from the seeds of Aleurites moluccanus and contains ricinoleic acid and alpha-elastosterol, offering excellent moisturizing and film-forming capabilities. When applied to the scalp, it is useful for preventing hair loss and restoring damaged hair through moisture retention and a hair-coating effect.

[0051] Grape seed oil is extracted from the seeds of Vitis vinifera and is rich in linoleic acid, OPC (proanthocyanidin), and vitamin E, promoting blood circulation and exhibiting powerful antioxidant effects. When applied to the scalp, it is excellent for promoting hair growth and preventing hair loss by stimulating hair follicle cell proliferation and inhibiting TGF-β.

[0052] Hydrolyzed keratin is a low-molecular-weight peptide obtained by hydrolyzing keratin proteins, such as those found in sheep or human hair, with acid or enzymatic hydrolysis. It has a structure similar to keratin, the main component of hair. Used as a hair conditioning agent in cosmetics, it forms a film on the surface of damaged hair to prevent static electricity and strengthens hair strength and elasticity, thereby aiding in the prevention of hair loss and the repair of damage.

[0053] Hydrolyzed collagen is a peptide produced by hydrolyzing animal collagen, offering excellent moisturizing and elasticity-enhancing effects. When applied to the scalp and hair, it supplies moisture and increases scalp elasticity to strengthen hair roots and delay hair loss, while increasing tensile strength and elongation to reduce breakage.

[0054] Hydrolyzed silk is produced by hydrolyzing silk protein from silkworm cocoons and is excellent for imparting softness and shine. It adds volume to dry and irritated hair, prevents static electricity, balances skin moisture, and improves hair smoothness and shine in scalp care.

[0055] Hydrolyzed elastin is a hydrolysate of animal elastin protein that restores elasticity and provides protection. It nourishes the hair and scalp to provide softness and strength, and strengthens hair weakened by hair loss by repairing damage and forming a protective barrier.

[0056] Hydrolyzed soy protein is a plant-based peptide produced by hydrolyzing soy protein, which has hair-strengthening and antioxidant effects. It replenishes protein to damaged hair to provide shine and bouncyness, maintains healthy hair texture by supplying moisture to the scalp, and makes limp hair voluminous.

[0057] Hydrolyzed corn protein is a hydrolysate of corn protein with excellent hair conditioning and protective functions. It helps improve damaged hair by coating the hair surface and increasing strength through nutrient supply, and enhances softness and elasticity in scalp care.

[0058] Leucine is one of the branched-chain amino acids (BCAAs) and is essential for protein synthesis and promoting hair growth. It stimulates the proliferation of hair follicle cells and supports energy metabolism, contributing to the extension of the hair growth phase and the prevention of hair loss, and is used as a hair strengthening agent in cosmetics.

[0059] Alanine is a non-essential amino acid that provides moisturizing and skin and hair conditioning effects. It reduces hair tangling and adds softness, and helps prevent hair loss by creating a healthy hair environment through maintaining scalp moisture balance.

[0060] Lysine is an essential amino acid that promotes the synthesis of keratin and collagen. It aids in hair growth and supports iron absorption, thereby alleviating iron deficiency, a cause of hair loss; it is also used in cosmetics for hair strengthening and scalp health.

[0061] Arginine is a semi-essential amino acid that helps activate hair follicles by improving blood flow and generating nitric oxide. By promoting scalp circulation and supporting keratin synthesis, it promotes hair growth and prevents hair loss.

[0062] Tyrosine is a non-essential amino acid involved in melanin synthesis and hair pigment formation. It maintains hair health by preventing graying and supporting cellular metabolism, and is utilized in cosmetics to strengthen hair.

[0063] Phenylalanine is an essential amino acid that contributes to strengthening hair structure as a tyrosine precursor. It aids in hair growth and prevents hair loss through protein synthesis and follicle stimulation.

[0064] Threonine is an essential amino acid with functions related to collagen synthesis and moisturization. It supports scalp and hair health by enhancing hair elasticity and maintaining moisture, and is effective in preventing hair loss-related weakening.

[0065] Proline is a non-essential amino acid and a major component of collagen. It helps promote hair growth and improve scalp elasticity, and is used in cosmetics as a moisturizer and structural strengthener.

[0066] Valine is a BCAA amino acid that aids in energy supply and protein synthesis. It contributes to preventing hair loss by improving hair shedding and nourishing hair follicles.

[0067] Isoleucine is a BCAA essential for muscle and hair protein synthesis. It is useful for repairing damaged hair by enhancing hair strength and supporting growth.

[0068] Histidine is an essential amino acid with antioxidant and moisturizing effects. It helps improve the hair loss environment by alleviating scalp inflammation and protecting hair.

[0069] Methionine is an essential amino acid that promotes the synthesis of keratin and collagen. It is effective for hair growth, structural strengthening, and preventing hair loss through its sulfur-containing bonds.

[0070] Glycine is a non-essential amino acid with excellent collagen synthesis and moisturizing properties. It contributes to preventing hair loss by supporting hair growth and maintaining scalp moisture balance.

[0071] Serine is a non-essential amino acid involved in moisturization and strengthening the skin barrier. It maintains healthy hair texture by providing softness and shine.

[0072] Cysteine ​​is a semi-essential amino acid and a key sulfur-containing amino acid for keratin synthesis. It is essential for preventing hair loss and enhancing hair strength by strengthening hair structure and forming disulfide bonds.

[0073] If the above composition is a cosmetic composition or a quasi-drug composition, it may be manufactured into any formulation conventionally produced in the industry. For example, in the case of a quasi-drug composition, it may be a scalp tonic, scalp lotion, scalp cream, scalp serum, scalp essence, scalp ampoule, scalp treatment, scalp conditioner, scalp shampoo, scalp pack, hair tonic, hair lotion, hair cream, hairspray, hair mousse, hair gel, hair conditioner, hair shampoo, hair rinse, hair pack, hair treatment, eyebrow growth product, eyelash growth product, eyelash serum, pet shampoo, pet rinse, hand sanitizer, detergent soap, soap, disinfectant cleaner, wet wipe, mask, ointment, patch, or filter filler, etc.

[0075] In addition, the above composition preferably further comprises 0.1 to 10 parts by weight of a functional extract, and more preferably further comprises 3 to 7 parts by weight.

[0076] The above functional extract is an extract obtained from a complex of black cumin seeds, Job's tears seeds, and quinoa mixed in a weight ratio of 1:1:1.

[0077] Black cumin seeds are small, black seeds of the Ranunculaceae plant Nigella sativa L., which is mainly cultivated in Southwest Asia and the Middle East, and possess a distinctive aroma and spicy taste. The seeds and their essential oils are rich in phytochemicals such as thymoquinone, thymohydroquinone, thymol, carvacrol, nigelidin, nigelisin, and α-hederin, and are reported to exhibit various physiological activities such as antioxidant, anti-inflammatory, and immune modulation effects. At the same time, they are evaluated as a food resource rich in protein and lipids, containing essential fatty acids, amino acids, vitamins, and minerals such as iron, calcium, potassium, zinc, phosphorus, and copper.

[0078] Job's tears (adlay seed) is the seed of Coix lacryma-jobi L., a perennial or annual plant belonging to the grass family (Gramineae, Poaceae). It forms distinctive glossy, oval or pear-shaped grains and has long been utilized in Asia as a traditional cereal and medicinal resource. Job's tears is not only a nutritious cereal containing a balanced amount of carbohydrates, proteins, and lipids, but it is also reported to exhibit pharmacological effects such as antioxidant, anti-inflammatory, lipid metabolism improvement, and immunomodulation, containing functional components such as benzoxazine derivatives, phenolic compounds, phytosterols, and various fatty acids.

[0079] Quinoa is the seed of the plant *Chenopodium quinoa Willd.*, which belongs to the family Amaranthaceae. Although it resembles cereals in appearance, it is taxonomically classified as a pseudocereal and is a highly nutritious grain resource that has been traditionally cultivated and consumed, primarily in the Andes region. Quinoa seeds are known to be rich in high-quality protein (balanced essential amino acids), complex carbohydrates, dietary fiber, and unsaturated fatty acids, as well as minerals such as calcium, magnesium, iron, and zinc, and antioxidant phytochemicals such as polyphenols, flavonoids, and tocopherols.

[0080] The composition according to the present invention can secure excellent hair loss prevention and hair growth promotion functionality by applying functional extracts extracted from black cumin seeds, Job's tears seeds, and quinoa.

[0081] In addition, the above functional extract is,

[0082] A step of washing black cumin seeds, Job's tears seeds, and quinoa, and heat-treating them at a temperature of 70-80°C for 20-40 minutes; a step of steaming the heat-treated black cumin seeds, Job's tears seeds, and quinoa at a pressure of 0.095-0.105 MPa and a temperature of 140-160°C for 80-100 minutes; a step of hot-air drying the steamed black cumin seeds, Job's tears seeds, and quinoa at a temperature of 58-62°C for 5-7 hours; A method prepared by performing the following steps: mixing 48-52 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa in a weight ratio of 1:1:1 with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a Bacillus coagulans strain, and fermenting at a temperature of 30-36°C for 2-4 days; after fermentation, adding 33-37 parts by weight of hexane and extracting at a temperature of 90-110°C for 8-10 hours to prepare an extract; and concentrating and freeze-drying the extract under reduced pressure.

[0083] Furthermore, the above functional extract is,

[0084] A step of washing black cumin seeds, Job's tears seeds, and quinoa, and heat-treating them at a temperature of 70-80°C for 20-40 minutes; a step of steaming the heat-treated black cumin seeds, Job's tears seeds, and quinoa at a pressure of 0.095-0.105 MPa and a temperature of 140-160°C for 80-100 minutes; a step of hot-air drying the steamed black cumin seeds, Job's tears seeds, and quinoa at a temperature of 58-62°C for 5-7 hours; a step of mixing 78-82 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa in a weight ratio of 1:1:1 with 18-22 parts by weight of mycelial powder, and fermenting and aging the mixture at a temperature of 25-35°C for 2-4 days; A method for preparing a product is applied by performing the following steps: obtaining a fermented product after fermentation and aging, mixing 48-52 parts by weight of the obtained fermented product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a Bacillus coagulans strain, and fermenting at a temperature of 30-36°C for 2-4 days; after fermentation, adding 33-37 parts by weight of hexane and extracting at a temperature of 90-110°C for 8-10 hours to prepare an extract; and concentrating and freeze-drying the extract under reduced pressure.

[0085] Higher functionality can be secured by applying a method prepared by fermenting and aging the raw material into a mycelial powder mixed with oyster mushrooms, poison ivy, and wheat bran in a weight ratio of 1:1:1, fermenting it using a Bacillus coagulans strain, and then extracting the result.

[0086] The above mycelial powder is prepared by washing oyster mushrooms, poison ivy, and wheat bran, drying them at a temperature of 48-52℃ for 8-10 hours, and grinding them.

[0088] In addition, the present invention

[0089] Awapuhi extract 0.1-10 parts by weight, caffeine 0.1-10 parts by weight, macadamia seed oil 0.1-10 parts by weight, olive oil 0.1-10 parts by weight, avocado oil 0.1-10 parts by weight, sunflower seed oil 0.1-10 parts by weight, soybean oil 0.1-10 parts by weight, annatto seed oil 0.1-10 parts by weight, argan kernel oil 0.1-10 parts by weight, drumstick seed oil 0.1-10 parts by weight, marula seed oil 0.1-10 parts by weight, camellia seed oil 0.1-10 parts by weight, pistachio seed oil 0.1-10 parts by weight, cucui seed oil 0.1-10 parts by weight, grape seed oil 0.1-10 parts by weight, cyclopentasiloxane 0.1-5.0 parts by weight, isopropyl myristate 0.1-5.0 parts by weight, dimethicone 0.1-5.0 parts by weight, phenyl trimethicone 0.1-5.0 parts by weight, isopropyl palmitate 0.1-5.0 parts by weight, hydrolyzed keratin 0.1-5.0 parts by weight, hydrolyzed collagen 0.1-5.0 parts by weight, hydrolyzed silk 0.1-5.0 parts by weight, hydrolyzed elastin 0.1-5.0 parts by weight, hydrolyzed soy protein 0.1-5.0 parts by weight, hydrolyzed corn protein 0.1-5.0 parts by weight, leucine 0.3-0.7 parts by weight, alanine 0.1-0.5 parts by weight, lysine 0.1-0.5 parts by weight, arginine 0.1-0.5 parts by weight, tyrosine 0.1-0.5 parts by weight, phenylalanine 0.1-0.5 parts by weight, threonine 0.1-0.5 parts by weight, proline 0.1-0.5 parts by weight, valine 0.1-0.5 parts by weight, isoleucine 0.1-0.5 parts by weight, histidine 0.1-0.5 parts by weight, methionine 0.1-0.5 parts by weight, glycine 0.1-0.5 parts by weight, serine 0.1-0.5 parts by weight, cysteine ​​0.1-0.5 parts by weight, caprylyl glycol 0.08-0.12 parts by weight, glutamic acid 0.08-0.12 parts by weight, aspartic acid 0.08-0.12 parts by weight, butylene glycol 1-3 parts by weight, ethylhexylglycerin 0.08-0.12 parts by weight, propanediol 0.08-0.12 parts by weight, tocopherol 0.08-0.12 parts by weight and 1,2-hexanediol 0.08-0.12 parts by weight.A method for preparing a composition for preventing hair loss and promoting hair growth is provided, comprising the step of mixing 12 parts by weight.

[0090] The composition according to the present invention is effective in preventing hair loss and promoting hair growth.

[0092] The present invention will be explained in more detail below by the following examples.

[0093] However, the following examples are merely illustrative of the content of the invention, and the scope of the invention is not limited by the examples and experimental examples.

[0094] <Preparation Example 1> Preparation of Functional Extract-1

[0095] Black cumin seeds, Job's tears seeds, and quinoa were washed and hot-air dried at a temperature of 60°C for 6 hours. 50 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa mixed in a weight ratio of 1:1:1 and 50 parts by weight of purified water were mixed, and 1 part by weight of a Bacillus coagulans strain was added, followed by fermentation at a temperature of 33°C for 3 days. After fermentation, 35 parts by weight of hexane were added, and an extract was prepared by extracting at a temperature of 100°C for 9 hours. The extract was concentrated under reduced pressure and freeze-dried to prepare a functional extract.

[0096] <Preparation Example 2> Preparation of Functional Extract-2

[0097] Black cumin seeds, Job's tears seeds, and quinoa were washed and heat-treated at 75°C for 30 minutes. The heat-treated black cumin seeds, Job's tears seeds, and quinoa were steamed at a pressure of 0.1 MPa and a temperature of 150°C for 90 minutes. The steamed black cumin seeds, Job's tears seeds, and quinoa were hot-air dried at 60°C for 6 hours. 50 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa mixed in a weight ratio of 1:1:1 and 50 parts by weight of purified water were mixed, and 1 part by weight of a Bacillus coagulans strain was added, followed by fermentation at 33°C for 3 days. After fermentation, 35 parts by weight of hexane were added, and the extract was prepared by extraction at 100°C for 9 hours. A functional extract was prepared by concentrating the above extract under reduced pressure and freeze-drying it.

[0098] <Preparation Example 3> Preparation of Functional Extract-3

[0099] Black cumin seeds, Job's tears seeds, and quinoa were washed and heat-treated at a temperature of 75°C for 30 minutes. The heat-treated black cumin seeds, Job's tears seeds, and quinoa were steamed at a pressure of 0.1 MPa and a temperature of 150°C for 90 minutes. The steamed black cumin seeds, Job's tears seeds, and quinoa were hot-air dried at a temperature of 60°C for 6 hours. 80 parts by weight of a composite of the hot-air dried black cumin seeds, Job's tears seeds, and quinoa mixed in a weight ratio of 1:1:1 and 20 parts by weight of mycelial powder were mixed and fermented and aged at a temperature of 30°C for 3 days. After fermentation and aging, a fermented and aged product was obtained. 50 parts by weight of the obtained fermented and aged product and 50 parts by weight of purified water were mixed, and 1 part by weight of a Bacillus coagulans strain was added, followed by fermentation at a temperature of 33°C for 3 days. After fermentation, 35 parts by weight of hexane were added, and an extract was prepared by extracting at a temperature of 100°C for 9 hours. The extract was concentrated under reduced pressure and freeze-dried to prepare a functional extract.

[0100] <Experimental Example 1> Analysis of Active Ingredient Content

[0101] In order to analyze the polyphenol content and flavonoid content of the functional extracts prepared in Preparation Examples 1-3 above, high-speed liquid chromatography (HPLC) analysis was performed, and the results are shown in Table 1 below.

[0102] Total polyphenol content (mg / g) Total flavonoid content (mg / g) Preparation Example 1 113.52 28.43 Preparation Example 2 128.58 30.12 Preparation Example 3 204.70 42.65

[0103] As shown in Table 1 above, it was confirmed that the functional extract according to the present invention has a high total polyphenol content and total flavonoid content.

[0104] <Example 1> Preparation of Composition-1

[0105] Awapuhi extract 5 parts by weight, caffeine 5 parts by weight, macadamia seed oil 5 parts by weight, olive oil 5 parts by weight, avocado oil 5 parts by weight, sunflower seed oil 5 parts by weight, soybean oil 5 parts by weight, annatto seed oil 5 parts by weight, argan kernel oil 5 parts by weight, drumstick seed oil 5 parts by weight, marula seed oil 5 parts by weight, camellia seed oil 5 parts by weight, pistachio seed oil 5 parts by weight, cucui seed oil 5 parts by weight, grape seed oil 5 parts by weight, cyclopentasiloxane 2.5 parts by weight, isopropyl myristate 2.5 parts by weight, dimethicone 2.5 parts by weight, phenyl trimethicone 2.5 parts by weight, isopropyl palmitate 2.5 parts by weight, hydrolyzed keratin 2.5 parts by weight, hydrolyzed collagen 2.5 parts by weight, hydrolyzed silk 2.5 parts by weight, hydrolyzed elastin 2.5 parts by weight, hydrolyzed soy protein 2.5 parts by weight, hydrolyzed corn protein 2.5 parts by weight, leucine 0.5 parts by weight, alanine 0.3 parts by weight, lysine 0.3 parts by weight, arginine 0.3 parts by weight, tyrosine 0.3 parts by weight, phenylalanine 0.3 parts by weight, threonine 0.3 parts by weight, proline 0.3 parts by weight, valine 0.3 parts by weight, isoleucine 0.3 parts by weight, histidine 0.3 parts by weight, methionine 0.3 parts by weight, glycine 0.3 parts by weight, serine 0.3 parts by weight, cysteine ​​0.3 parts by weight, caprylyl glycol 0.1 parts by weight, glutamic acid A composition was prepared by mixing 0.1 parts by weight of aspartic acid, 0.1 parts by weight of butylene glycol, 0.1 parts by weight of ethylhexylglycerin, 0.1 parts by weight of propanediol, 0.1 parts by weight of tocopherol, and 0.1 parts by weight of 1,2-hexanediol.

[0106] <Example 2> Preparation of Composition-2

[0107] A composition was prepared by performing the same procedure as in Example 1, except that 5 parts by weight of the functional extract prepared in Preparation Example 1 were further mixed in the above Example 1.

[0108] <Example 3> Preparation of Composition-3

[0109] A composition was prepared by performing the same procedure as in Example 1, except that 5 parts by weight of the functional extract prepared in Preparation Example 3 were further mixed in Example 1.

[0110] <Experimental Example 4> Analysis of Hair Loss Prevention or Treatment Functionality

[0111] The functionality of the composition prepared in Examples 1-3 above for preventing or treating hair loss was confirmed through the following experiment.

[0112] 1. Measurement of dermal papilla cell viability

[0113] To evaluate the viability of hair follicle tissue and related cells upon treatment with the compositions prepared in Examples 1-3 above, the WST-1 kit was used. Hair papilla cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin at 37°C in a 5% CO2 incubator. The cultured cells were seeded into a 96-well plate (1 x 10⁶ 4After cell / well, the cells were cultured again for 24 hours in a 37°C 5% CO2 incubator. Subsequently, each well was treated with 100 mg / g of each composition and cultured for 48 hours. Next, the WST-1 reaction solution was diluted 1 / 10 in a medium without supplements, and 100 µl was added to each well. The mixture was placed in a 37°C incubator, and after 1 hour, the absorbance was measured at 450 nm using an ELISA microplate reader (SoftMax Pro5, Molecular Devices, USA). Additionally, the same dermal papilla cells were treated with only DMSO (dimethyl sulfoxide) to serve as a control group, and a 10 μM minoxidil powder formulation (not the composition of the above example) was treated to serve as a positive control group. The absorbance value of the control group was set to 100%, and the relative absorbances of the positive control group and the samples were calculated; the results are shown in Table 2 below.

[0114] Cell viability (% of control group) control group 100 positive control group 111 Example 1 106 Example 2 106 Example 3 109

[0115] As shown in Table 2 above, the composition according to the present invention does not cause damage to hair papilla cells, so it can be confirmed that it does not reduce the viability of hair papilla cells and is not cytotoxic.

[0116] 2. Confirmation of expression levels of hair loss-related cytokines

[0117] 2×10 matrix cells 5Cells were dispensed into a 6-well plate at a rate of cells / mL and treated simultaneously with LPS (400 ng / mL) and the composition of the above example, respectively. The cells were then cultured for 24 hours at 37°C under 5% CO2 conditions. An ELISA (Enzyme-linked immunosorbent assay) was performed using each cell culture medium to analyze the production of TNF-α. The absorbance was measured at 450 nm, and the protein production amount was calculated and converted into a percentage, which is shown in Table 3 below. The control group, which received no treatment, was set as 100% for comparison.

[0118] TNF-α production (%) Control group (LPS 400 ng / ㎖) 480.4 Example 1 157.4 Example 2 112.3 Example 3 108.0

[0119] As shown in Table 3 above, it was confirmed that when the composition according to the present invention is applied, the protein expression of the inflammatory cytokine TNF-α in LPS-treated matrix cells is reduced.

[0120] 3. Hair follicle tissue culture evaluation

[0121] To confirm the hair growth effect of the composition prepared in Examples 1-3 above, hair follicle tissue was cultured. Specifically, hair follicle tissue from a male contributor biopsied from the occipital region was cleaned for use in the experiment and then cultured in a 37°C, 5% CO2 incubator using Serum-free William's medium (Invitrogen) containing 1% penicillin, 2 mM glutamine, and 10 ng / mL hydrocortisone.

[0122] Next, hair follicle tissues from male contributors that were undamaged and in the growth phase (anagen-hair cycle) were isolated using a microscope and used in the experiment. Five strands were formed per experimental group and cultured in a medium placed in a 24-well plate. At this time, each composition of the above example was added to each well at a concentration of 100 mg / g. After a total culture period of 6 days, the hair growth length was measured by microscopic imaging, and the average value was calculated. In addition, hair follicle tissues that were not treated with anything were set as a control group, and hair follicle tissues treated with a 10 μM minoxidil powder formulation (not the above sample) were set as a positive control group and cultured under the same conditions. Subsequently, the hair growth lengths of the control and positive control groups were measured, and the average values ​​were calculated; the results are shown in Table 4 below.

[0123] Average growth length (μm) control group 20.5 positive control group 25.7 Example 1 26.0 Example 2 31.4 Example 3 39.7

[0124] As shown in Table 4 above, it was confirmed that the degree of hair growth was excellent when the composition according to the present invention was applied.

Claims

Claim 1 Awapuhi extract 0.1-10 parts by weight, functional extract 0.1-10 parts by weight, caffeine 0.1-10 parts by weight, macadamia seed oil 0.1-10 parts by weight, olive oil 0.1-10 parts by weight, avocado oil 0.1-10 parts by weight, sunflower seed oil 0.1-10 parts by weight, soybean oil 0.1-10 parts by weight, annatto seed oil 0.1-10 parts by weight, argan kernel oil 0.1-10 parts by weight, drumstick seed oil 0.1-10 parts by weight, marula seed oil 0.1-10 parts by weight, camellia seed oil 0.1-10 parts by weight, pistachio seed oil 0.1-10 parts by weight, cucui seed oil 0.1-10 parts by weight, grape seed oil 0.1-10 parts by weight, 0.1-5.0 parts by weight of cyclopentasiloxane, 0.1-5.0 parts by weight of isopropyl myristate, 0.1-5.0 parts by weight of dimethicone, 0.1-5.0 parts by weight of phenyltrimethicone, 0.1-5.0 parts by weight of isopropyl palmitate, 0.1-5.0 parts by weight of hydrolyzed keratin, 0.1-5.0 parts by weight of hydrolyzed collagen, 0.1-5.0 parts by weight of hydrolyzed silk, 0.1-5.0 parts by weight of hydrolyzed elastin, 0.1-5.0 parts by weight of hydrolyzed soy protein, 0.1-5.0 parts by weight of hydrolyzed corn protein, 0.3-0.7 parts by weight of leucine, 0.1-0.5 parts by weight of alanine, and 0.1-0.5 parts by weight of lysine. parts by weight, arginine 0.1-0.5 parts by weight, tyrosine 0.1-0.5 parts by weight, phenylalanine 0.1-0.5 parts by weight, threonine 0.1-0.5 parts by weight, proline 0.1-0.5 parts by weight, valine 0.1-0.5 parts by weight, isoleucine 0.1-0.5 parts by weight, histidine 0.1-0.5 parts by weight, methionine 0.1-0.5 parts by weight, glycine 0.1-0.5 parts by weight, serine 0.1-0.5 parts by weight, cysteine ​​0.1-0.5 parts by weight, caprylyl glycol 0.08-0.12 parts by weight, glutamic acid 0.08-0.12 parts by weight, aspartic acid 0.08-0.12 parts by weight, butylene glycol 1-3 parts by weight, 0.08-0.12 parts by weight of ethylhexylglycerin, 0.08-0.12 parts by weight of propanediol, and 0.08-0.12 parts by weight of tocopherol.The functional extract comprises 12 parts by weight of and 0.08-0.12 parts by weight of 1,2-hexanediol, and comprises the steps of: washing black cumin seeds, Job's tears seeds, and quinoa, and heat-treating them at a temperature of 70-80°C for 20-40 minutes; steaming the heat-treated black cumin seeds, Job's tears seeds, and quinoa at a pressure of 0.095-0.105 MPa and a temperature of 140-160°C for 80-100 minutes; and hot-air drying the steamed black cumin seeds, Job's tears seeds, and quinoa at a temperature of 58-62°C for 5-7 hours. A step of mixing 78-82 parts by weight of a complex of hot-air dried black cumin seeds, Job's tears seeds, and quinoa in a weight ratio of 1:1:1 with 18-22 parts by weight of mycelial powder, and fermenting and aging at a temperature of 25-35℃ for 2-4 days; a step of obtaining a fermented and aged product after fermentation and aging, mixing 48-52 parts by weight of the obtained fermented and aged product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a Bacillus coagulans strain, and fermenting at a temperature of 30-36℃ for 2-4 days; a step of preparing an extract by adding 33-37 parts by weight of hexane after fermentation and extracting at a temperature of 90-110℃ for 8-10 hours. A composition for preventing hair loss and promoting hair growth, characterized by being prepared by performing the step of concentrating and freeze-drying the extract; wherein the mycelial powder is prepared by washing oyster mushrooms, poison ivy, and wheat bran, drying them at a temperature of 48-52℃ for 8-10 hours, and grinding them. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 delete

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