A cosmetic composition comprising collagen

Abstract

The present invention provides a cosmetic composition comprising collagen, a peptide mixture, and a vitamin A derivative, thereby providing a cosmetic composition containing a stable active ingredient that is effective in preventing and improving skin wrinkles and synthesizing collagen.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition containing collagen. Background Technology

[0003] Human skin undergoes intrinsic aging as time passes, resulting from a decrease in hormone secretion and a decline in the function and activity of immune cells, which in turn reduces the biosynthesis of biological proteins. Furthermore, external factors such as dry air, pollutants, and ultraviolet rays cause the skin to thin, wrinkles to increase, and elasticity to decrease, leading to aging phenomena such as an increase in melasma, freckles, and age spots.

[0004] External factors affecting skin aging include wind, temperature, humidity, cigarette smoke, pollution, and ultraviolet rays. These factors lead to a significant decrease in collagen, the main protein of skin tissue, and matrix metalloproteinase-1 (MMP-1) plays a crucial role in this collagen reduction. MMP-1 is an enzyme involved in the degradation of the extracellular matrix and basement membrane. Research has reported that UV irradiation increases MMP-1 activity within the skin, causing significant collagen breakdown. Consequently, MMP-1s influence the breakdown of collagen in the dermis, playing a vital role in wrinkle formation.

[0005] Therefore, there is a need to develop substances capable of regulating or inhibiting the expression of MMPs that induce activation within cells. Until now, most raw materials used as cosmetic ingredients have merely inhibited enzyme activity. Currently developed active ingredients for skin wrinkle improvement often cannot be used as cosmetic ingredients due to cytotoxicity or are highly unstable, requiring special stabilization systems. Furthermore, their high molecular weight makes delivery to the skin difficult, necessitating special delivery systems, and they suffer from the problem of not providing visible wrinkle improvement effects. Consequently, interest is increasingly focusing on skin protectants containing retinoids, which are currently utilized as a means to address photoaging phenomena such as wrinkles, skin thickening, sagging, and loss of elasticity resulting from sun exposure. However, retinoids are highly unstable compounds that are sensitive to ultraviolet rays, moisture, heat, and oxygen, causing them to undergo chemical changes easily, which limits their use.

[0006] Therefore, research is currently focused on developing stable active ingredients that are effective for preventing and improving skin wrinkles and for collagen synthesis. Prior art literature

[0008] 1. Republic of Korea Published Patent Application No. 1020240068259 2. Republic of Korea Registered Patent Application No. 10-2147833 The problem to be solved

[0009] The present invention, for solving the problems described above, provides a cosmetic composition comprising an active ingredient that is effective and stable for preventing and improving skin wrinkles and for collagen synthesis.

[0010] The present invention provides a cosmetic composition comprising collagen, a peptide mixture, and a vitamin A derivative, wherein the peptide mixture comprises beta-alanylhydroxyprolyldiaminobutyroylbenzylamide, acetyltetrapeptide-9, oligopeptide-1, and hexapeptide-11.

[0011] The problems that the present invention aims to solve are not limited to those mentioned above, and other unmentioned problems will be clearly understood by a person skilled in the art from the description below. means of solving the problem

[0013] The present invention, for solving the above-mentioned problem, provides a cosmetic composition comprising collagen, a peptide mixture, and a vitamin A derivative, wherein the peptide mixture comprises beta-alanylhydroxyprolyldiaminobutyroylbenzylamide, acetyltetrapeptide-9, oligopeptide-1, and hexapeptide-11.

[0014] According to one embodiment of the present invention, the vitamin A derivative comprises one or more selected from all-trans retinol, retinol, retinal, retinyl acetate, retinalaldehyde, retinyl palmitate, retinoic acid, retinyl propionate, retinyl linoleate, dehydroretinol, and hydroxypinacolone retinoate.

[0015] According to one embodiment of the present invention, the cosmetic composition comprises 0.0000001 to 0.05 weight% of the peptide mixture, 0.0000001 to 0.05 weight% of a vitamin A derivative, and 0.0000001 to 0.05 weight% of collagen based on the total weight of the cosmetic composition.

[0016] According to one embodiment of the present invention, the cosmetic composition provides a cosmetic composition for preventing skin aging, preventing or improving skin wrinkles, maintaining or enhancing skin elasticity, or promoting collagen biosynthesis.

[0017] According to one embodiment of the present invention, the cosmetic composition provides a cosmetic composition formulated into one or more selected from the group consisting of softening lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. Effects of the invention

[0019] According to the present invention as described above, a cosmetic composition is provided that includes an active ingredient that is effective and stable for preventing and improving skin wrinkles and for collagen synthesis.

[0020] The effects of the present invention are not limited to those mentioned above, and other unmentioned effects will be clearly understood by a person skilled in the art from the description below. Brief explanation of the drawing

[0022] Figure 1 is a graph showing the results of the evaluation of collagen synthesis promotion of the entire composition of the example and comparative example according to the present invention. Figure 2a is a graph showing the results of the evaluation of collagen synthesis promotion according to changes in collagen content. Figure 2b is a graph showing the results of the evaluation of collagen synthesis promotion according to changes in the peptide mixture content. Figure 2c is a graph showing the results of the evaluation of collagen synthesis promotion according to changes in hydroxypinacolone retinoate content. FIG. 3a is a graph showing the results of the collagen synthesis promotion evaluation of the composition of Example 2 according to the present invention in comparison with Comparative Examples 7, 8, and 9. FIG. 3b is a graph showing the results of the collagen synthesis promotion evaluation of the composition of Example 3 according to the present invention in comparison with Comparative Examples 10, 11, and 12. Specific details for implementing the invention

[0023] The advantages and features of the present invention and the methods for achieving them will become clear by referring to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below but may be implemented in various different forms. These embodiments are provided merely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the present invention, and the present invention is defined only by the scope of the claims.

[0024] The terms used in this specification are for describing embodiments and are not intended to limit the invention. In this specification, the singular form includes the plural form unless specifically stated otherwise in the text. The terms "comprises" and / or "comprising" used in this specification do not exclude the presence or addition of one or more other components in addition to the components mentioned. Throughout the specification, the same reference numerals refer to the same components, and "and / or" includes each of the mentioned components and all combinations of one or more. Although terms such as "first," "second," etc., are used to describe various components, these components are not limited by these terms. These terms are used merely to distinguish one component from another. Therefore, the first component mentioned below may be the second component within the technical scope of the invention.

[0025] Unless otherwise defined, all terms used herein (including technical and scientific terms) may be used in a meaning commonly understood by those skilled in the art to which the present invention pertains. Additionally, terms defined in commonly used dictionaries are not to be interpreted ideally or excessively unless explicitly and specifically defined otherwise.

[0027] The present invention provides a cosmetic composition comprising collagen, a peptide mixture, and a vitamin A derivative, wherein the peptide mixture comprises beta-alanylhydroxyprolyldiaminobutyroylbenzylamide, acetyltetrapeptide-9, oligopeptide-1, and hexapeptide-11.

[0028] "Collagen" is a structural protein found in the skin, connective tissue, bone, and other tissues of animals. Collagen is a protein composed of a triple helix structure, and has non-helical telopeptides at both ends of the triple helix structure. In the present invention, collagen may be derived from humans, cattle, pigs, mice, rats, etc., but is not limited to such origin. In the present invention, collagen may be not only natural collagen but also biotechnologically modified collagen or biotechnologically or chemically treated modified collagen, but is not limited to its origin or modification.

[0029] In the present invention, the collagen may be either natural collagen or synthetic collagen, and specifically, may be human collagen or a cleavage thereof, collagen of an animal other than a human or a delivery thereof, or a recombinant polypeptide. More specifically, it may be human collagen, and even more specifically, it may be a protein comprising the whole or part of the sequence of human collagen type 3 peptide (HU3).

[0030] "Beta-alanylhydroxyprolyl diaminobutyroylbenzylamide" is a compound having the following structural formula. Through this, it can exert an effect of generating collagen.

[0031]

[0032] Acetyltetrapeptide-9 is a peptide composed of aspartic acid, glutamine, histidine, and valine, and is a product obtained from the acetylation reaction of tetrapeptide-9. Through this, it can exert the effect of strengthening collagen bonds.

[0033] "Oligopeptide-1" is an epidermal growth factor that is naturally present in humans and binds to receptors on the surface of the skin to promote the production of new cells. It is a type of protein formed from 53 amino acids in the body and is a synthetic peptide composed of glycine, histidine, and lysine residues. Through this, it can exert an effect that inhibits the secretion of MMPs.

[0034] "Hexapeptide-11" is a synthetic peptide composed of alanine, phenylalanine, proline, and valine. Through this, it can exert an effect that activates autophagy function.

[0035] It is most desirable to use these peptides together with the peptides listed above rather than using them alone, in order to maximize their skin improvement effects.

[0036] According to one embodiment of the present invention, the vitamin A derivative comprises all vitamin A derivatives that can be used dermatologically. Specifically, it comprises one or more selected from all-trans retinol, retinol, retinal, retinyl acetate, retinalaldehyde, retinyl palmitate, retinoic acid, retinyl propionate, retinyl linoleate, dehydroretinol, and hydroxypinacolone retinoate. Specifically, the vitamin A derivative may be hydroxypinacolone retinoate. This enables the production of collagen and the inhibition of collagenase.

[0037] According to one embodiment of the present invention, the cosmetic composition comprises 0.0000001 to 0.05 weight% of the peptide mixture, 0.0000001 to 0.05 weight% of a vitamin A derivative, and 0.0000001 to 0.05 weight% of collagen based on the total weight of the cosmetic composition.

[0038] The above peptide mixture may be included in an amount of 0.0000001 to 0.05 weight% relative to the total weight of the cosmetic composition. Specifically, it may be included in an amount of 0.000001 to 0.05 weight%, more specifically, 0.00005 to 0.05 weight%, even more specifically, 0.0005 to 0.05 weight%, and even more specifically, 0.005 to 0.05 weight%.

[0039] The above vitamin A derivative may be included in an amount of 0.0000001 to 0.05 weight% based on the total weight of the cosmetic composition. Specifically, 0.000001 to 0.05 weight%, more specifically 0.00005 to 0.05 weight%, even more specifically 0.0005 to 0.05 weight%, and even more specifically 0.005 to 0.05 weight%.

[0040] The above collagen may be included in an amount of 0.0000001 to 0.05 weight% based on the total weight of the cosmetic composition. Specifically, 0.000001 to 0.05 weight%, more specifically 0.00005 to 0.05 weight%, even more specifically 0.0005 to 0.05 weight%, and even more specifically 0.005 to 0.05 weight%.

[0041] According to one embodiment of the present invention, the cosmetic composition provides a cosmetic composition for preventing skin aging, preventing or improving skin wrinkles, maintaining or enhancing skin elasticity, or promoting collagen biosynthesis.

[0042] In addition to the above composition, the cosmetic composition of the present invention may additionally include ingredients commonly used in cosmetic compositions, such as preservatives, stabilizers, surfactants, thickeners, solvents, moisturizers, emollients, UV absorbers, preservatives, disinfectants, emulsifiers, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, cooling agents, and limiting agents.

[0043] The cosmetic composition of the present invention may be prepared in any formulation conventionally manufactured in the art, for example, as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. More specifically, it may be prepared in various forms such as a solution, sol-gel, emulsion, oil, wax, aerosol, etc., such as a softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, powder, hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, gel, etc., but is not limited thereto.

[0044] According to one embodiment of the present invention, the cosmetic composition provides a cosmetic composition formulated by being selected from the group consisting of softening lotion, astringent lotion, nourishing lotion, skin, lotion, cream, nourishing cream, massage cream, eye cream, essence, serum, gel, water-soluble liquid, pack, makeup base, BB cream, foundation, lipstick, lip balm, lip tint, lip gloss, sun cream, sun lotion, sun milk, sun stick, cleansing cream, cleansing foam, cleansing water, cleansing oil, pack, spray, and powder.

[0046] Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings.

[0048] <Preparation of Examples and Comparative Examples>

[0049] The compositions of the Examples and Comparative Examples were prepared by diluting and mixing the components with DMSO (Dimethyl Sulfoxide) at the content (unit D, μg / ml) of Table 1 below. Specifically, the components were vortexed with DMSO for at least 10 seconds to ensure complete mixing and dissolution. When treating cells, the mixture was left at room temperature for at least 30 minutes, then re-mixed by pipetting at least 10 times before cell treatment.

[0050] Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Comparative Example 11 Comparative Example 12 Example 1 Example 2 Example 3 Example 4 Peptide mixture (PM) - - 0.1 0.5 - - 0.1 - 0.1 0.5 0.5 - 0.1 0.1 0.5 0.5 Hydroxyfinacolone retinoate (HPR) - - - - 0.1 0.5 - 0.1 0.1 0.5 - 0.1 0.1 0.1 0.5 0.5 Collagen (HU3) 1 5 - - - - 5 5 - - 1 1 1 5 1 5

[0051] The above peptide mixture used a mixture of beta-alanylhydroxyprolyldiaminobutyroylbenzylamide, acetyltetrapeptide-9, oligopeptide-1, and hexapeptide-11.

[0052] The above hydroxypinacolone retinoate was used with a molecular weight of 398.6 g / mol.

[0053] The above collagen was used as human-like collagen that matches the sequence of human collagen type 3.

[0055] <Experimental Example - Evaluation of Collagen Synthesis Promotion>

[0056] 1×10⁶ human skin fibroblasts (HDF cells) in a 12-well plate 5 Cells were inoculated at a rate of cells / well and cultured for 24 hours. After one wash with 0.5 mL of 1X PBS, the medium was replaced with 0.1% FBS DMEM. After 4 hours, the samples of the above Examples and Comparative Examples were treated, and the culture was continued for another 48 hours to collect the cell culture supernatant. The amount of collagen in the cell culture supernatant was quantified using a procollagen type I C-peptide (PICP) EIA kit (Takara Bio, Japan). The measured collagen amount was corrected for the total protein amount obtained by the Lowry assay. Collagen expression rate was calculated using the following formula, and an untreated group was used as the control. The experimental result for each sample is the average of three measurements.

[0057]

[0058] (1) Overall experimental results

[0059] The overall experimental results for the above samples are shown in Table 2 below.

[0060] division Collagen expression rate (%) Comparative Example 1 154.98 Comparative Example 2 180.36 Comparative Example 3 141.33 Comparative Example 4 142.87 Comparative Example 5 125.23 Comparative Example 6 128.10 Comparative Example 7 176.27 Comparative Example 8 173.30 Comparative Example 9 158.21 Comparative Example 10 154.42 Comparative Example 11 165.65 Comparative Example 12 162.78 Example 1 171.28 Example 2 298.10 Example 3 193.45 Example 4 252.82

[0061] The graph of the above results is shown in Fig. 1. A t-test was performed on the experimental results and the results are displayed (*, p<0.05, **, p<0.01, ***, p<0.001). Through this, it was confirmed that the composition of the embodiment of the present invention exhibits an excellent collagen production rate.

[0063] (2) Comparison of each sample group

[0064] When comparing Comparative Example 1 (1 μg / ml) and Comparative Example 2 (5 μg / ml), which differed only in collagen content, the collagen production rate increased by 116.4% with the increase in collagen content (Fig. 2a).

[0065] When comparing Comparative Example 3 (0.1 μg / ml) and Comparative Example 4 (0.5 μg / ml), which differed only in the content of the peptide mixture, the collagen production rate increased by 101.1% with the increase in the peptide mixture content (Fig. 2b).

[0066] When comparing Comparative Example 5 (0.1 μg / ml) and Comparative Example 6 (0.5 μg / ml), which differed only in the content of hydroxypinacolone retinoate, the collagen production rate increased by 103.3% with the increase in hydroxypinacolone retinoate content (Fig. 2c).

[0067] Comparing Example 2 with Comparative Examples 7, 8, and 9, all of the comparative examples contain only two combinations of the peptide mixture, hydroxypinacolone retinoate, and collagen, although they have the same concentration as Example 2. When compared, Example 2 can be seen to have an increase in collagen synthesis rate of at least 169.1% to 188.4% compared to these comparative examples (Fig. 3a).

[0068] When comparing Example 3 with Comparative Examples 10, 11, and 12, all of the above comparative examples contain only two combinations of a peptide mixture, hydroxypinacolone retinoate, and collagen compared to Example 3. When comparing them, it can be confirmed that Example 3 shows an increase in collagen synthesis rate of at least 116.80% to 125.3% compared to these comparative examples (Fig. 3b).

[0069] Although embodiments of the present invention have been described above with reference to the attached drawings, those skilled in the art will understand that the present invention may be implemented in other specific forms without altering its technical concept or essential features. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive.

Claims

Claim 1 A cosmetic composition comprising collagen, a peptide mixture, and a vitamin A derivative, wherein the peptide mixture comprises beta-alanylhydroxyprolyldiaminobutyroylbenzylamide, acetyltetrapeptide-9, oligopeptide-1, and hexapeptide-11. Claim 2 A cosmetic composition according to claim 1, wherein the vitamin A derivative comprises one or more selected from all-trans retinol, retinol, retinal, retinyl acetate, retinalaldehyde, retinyl palmitate, retinoic acid, retinyl propionate, retinyl linoleate, dehydroretinol, and hydroxypinacolone retinoate. Claim 3 The cosmetic composition of claim 1, wherein the cosmetic composition comprises 0.0000001 to 0.05 weight% of the peptide mixture, 0.0000001 to 0.05 weight% of a vitamin A derivative, and 0.0000001 to 0.05 weight% of collagen based on the total weight of the cosmetic composition. Claim 4 In claim 1, the cosmetic composition is a cosmetic composition for preventing skin aging, preventing or improving skin wrinkles, maintaining or enhancing skin elasticity, or promoting collagen biosynthesis. Claim 5 In claim 1, the cosmetic composition is formulated into one or more selected from the group consisting of softening lotion, astringent lotion, nourishing lotion, skin toner, lotion, cream, nourishing cream, massage cream, eye cream, essence, serum, gel, water-soluble liquid, pack, makeup base, BB cream, foundation, lipstick, lip balm, lip tint, lip gloss, sun cream, sun lotion, sun milk, sun stick, cleansing cream, cleansing foam, cleansing water, cleansing oil, pack, spray, and powder.

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