A Specialized nutrient solution for hydroponic cultivation of coriander and a method for cultivating coriander using the same

Abstract

The present invention relates to a dedicated nutrient solution and a cultivation method for hydroponic cultivation of coriander. The coriander-dedicated nutrient solution of the present invention is composed of a ratio of inorganic ions preferred by coriander and is adjusted to a specific level of electrical conductivity, thereby having the effect of inducing relatively superior coriander growth promotion compared to commercially available nutrient solutions generally used for coriander cultivation.

Description

Technology Field

[0001] The present invention relates to a dedicated culture solution for hydroponic cultivation of coriander and a method for cultivating coriander using the same. Background Technology

[0002] A vertical farm is a system capable of stably producing large quantities of crops year-round by precisely controlling environmental factors such as temperature, humidity, light, carbon dioxide, and nutrients. To enhance profitability in the vertical farming industry, recent attempts have been made to produce high-quality crops, such as functional vegetables and medicinal plants, for the fields of plant-based health functional foods and food and pharmaceuticals; precise environmental control is essential for the stable mass production of these crops.

[0003] master( Coriandrum sativum Cilantro is a herb widely used in cooking and medicine around the world due to its distinctive aroma and taste, and recently, its value as a health functional food has also been rising. If stable mass production is possible in vertical farms, cilantro is a promising crop from which high income can be expected.

[0004] To establish a stable mass production system for cilantro in vertical farms, the development of cultivation and environmental control technologies is essential. Since vertical farms produce crops through hydroponics, achieving hydroponic cultivation for cilantro is a priority for production; furthermore, because different crops have different preferences for specific nutrients, it is necessary to develop nutrient solutions specifically tailored to the target crop.

[0005] In particular, while conventional hydroponic nutrient solutions contain minerals necessary for crop growth and development, general-purpose solutions for various horticultural crops do not take into account the unique nutrient preferences of each crop, which may result in slow growth rates or poor quality when cultivating coriander.

[0006] In addition, since low nutrient solution concentrations induce poor growth and high concentrations limit nutrient absorption due to osmotic stress, selecting the appropriate concentration is also an important factor.

[0007] As prior art regarding a culture solution for promoting plant growth and a cultivation method using the same, Korean Published Patent No. 10-2014-0035821 discloses a nutrient solution composition for hydroponic cultivation of ice plants and a plant factory system using said composition. It states that the composition is formulated based on the macronutrient components of ice plants and can significantly improve the productivity of ice plants compared to existing nutrient solution compositions, making it useful for mass cultivation.

[0008] In addition, Korean registered patent No. 10-1738541 discloses a method for cultivating plants of the genus *Godeulppaegi* and a nutrient solution for the same, stating that by using this, high-functional *Godeulppaegi* plants with high medicinal utility can be efficiently cultivated in facilities such as plant factories.

[0009] In addition, Chinese published patent CN 104163721 A discloses a hydroponic cultivation solution capable of improving the growth rate of coriander belonging to the parsley family, and states that using this can increase the auxin levels of coriander, promote the longitudinal growth of cells and the growth of internode cells in the direction of stems and leaves, make the leaves long and soft, and prevent excessive growth when an appropriate amount is used.

[0010] However, it is difficult to apply the cultivation methods of ice plant and *Lactuca indica* disclosed in prior literature to the cultivation of coriander, and these documents do not mention a culture solution with a composition similar to the ratio of inorganic ions in coriander in relation to a hydroponic solution capable of improving the growth rate of coriander.

[0011] The inventors completed the present invention by confirming that a culture solution composition having a composition similar to the ratio of inorganic ions preferred by coriander can induce relatively superior coriander growth promotion compared to commercially available nutrient solutions generally used in conventional coriander cultivation. The problem to be solved

[0012] The objective of the present invention is to provide a culture solution composition for promoting coriander growth that is similar in composition to the ratio of inorganic ions preferred by coriander and is adjusted to a specific level of electrical conductivity, thereby inducing relatively superior coriander growth promotion compared to conventional ash nutrient solutions.

[0013] The present invention provides a method for cultivating coriander using a culture solution composition for promoting coriander growth. means of solving the problem

[0014] To achieve the above objective, the present invention,

[0015] A first culture solution containing 45 to 55 parts by weight of potassium nitrate (KNO3), 65 to 75 parts by weight of calcium nitrate (Ca(NO3)2·4H2O), and 0.6 to 1.6 parts by weight of iron (Fe-EDTA); and

[0016] A second culture solution comprising 45 to 55 parts by weight of potassium nitrate (KNO3), 4.0 to 10.0 parts by weight of diammonium phosphate (NH4H2PO4), 10.0 to 16.0 parts by weight of potassium phosphate (KH2PO4), 14 to 20 parts by weight of magnesium sulfate (MgSO4·7H2O), 0.01 to 0.03 parts by weight of copper sulfate (CuSO4·5H2O), 0.1 to 0.3 parts by weight of boron (H3BO3), 0.1 to 0.3 parts by weight of manganese sulfate (MnSO4·5H2O), 0.1 to 0.3 parts by weight of zinc sulfate (ZnSO4·7H2O), and 0.01 to 0.03 parts by weight of sodium molybdate (Na2MoO4·2H2O); characterized by comprising a high-quality culture solution. A culture medium composition for promoting growth is provided. Effects of the invention

[0017] The present invention has a composition similar to the ratio of inorganic ions preferred by coriander and is adjusted to a specific level of electrical conductivity, thereby having the effect of inducing relatively superior growth promotion of coriander compared to commercially available nutrient solutions generally used for coriander cultivation. Brief explanation of the drawing

[0018] Figure 1 is a figure showing the results of comparing the ratios of cations K, Ca, Mg and anions N, P, S in a nutrient solution dedicated to high-quality plants (New), a commercially available nutrient solution (MH), and a high-quality plant (Plant) according to the present invention. Figure 2 is a graph showing the growth characteristics of coriander grown through treatment with an ash nutrient solution (M) and a coriander-specific culture solution (New) having different electrical conductivity (EC). (a) is a graph showing tip burn rate, SPAD, crown diameter, and leaf area; (b) is a graph showing root fresh weight and root length; and (c) is a graph showing shoot fresh weight, shoot dry weight, and shoot length. Figure 3 shows the degree of change in pH and electrical conductivity (EC) according to the electrical conductivity of a commercially available nutrient solution (M) and a nutrient solution for high-quality vegetables (New) during the cultivation period. (a) is a graph showing the degree of change in pH; and (b) is a graph showing the degree of change in EC. Figure 4 is a photograph showing the external growth results of coriander grown with different electrical conductivity concentrations of commercial nutrient solution (M) and coriander-specific nutrient solution (New). Specific details for implementing the invention

[0019] The present invention will be described in detail below.

[0020] As used in this specification, the term 'coriander' refers to an annual herb belonging to the Apiaceae family, commonly known as coriander or cilantro. Coriander is a plant whose leaves, stems, and seeds are all edible and is widely used in cooking, particularly in Asia, South America, and the Middle East, and is characterized by having a unique aroma as a spice and herb.

[0021] Coriander can be broadly divided into the above-ground part (shoot) and the underground part (root). The above-ground part consists of leaves and stems; the stems grow in a thin, somewhat upright form and form branches. The leaves take on two forms: young leaves are flat with soft edges, while as they age, they become thinner and change into a feather-like shape. Coriander leaves and stems have a strong aroma and fresh flavor, making them widely used as herbs to enhance the flavor of food.

[0022] The underground part (root) of coriander is long and thin, and has a brown, fibrous structure that grows relatively deep underground.

[0023] As used in this specification, the term "culture solution" refers to a liquid solution containing inorganic ions and water, etc., necessary for plant growth, and the term "culture solution" may be used interchangeably with the term "nutrient solution." In addition to inorganic ions and water, the culture solution may contain components that promote the growth and development of coriander.

[0024] As used herein, the term "includes" means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0025] The present invention is,

[0026] A first culture solution containing 45 to 55 parts by weight of potassium nitrate (KNO3), 65 to 75 parts by weight of calcium nitrate (Ca(NO3)2·4H2O), and 0.6 to 1.6 parts by weight of iron (Fe-EDTA); and

[0027] A second culture solution comprising 45 to 55 parts by weight of potassium nitrate (KNO3), 4.0 to 10.0 parts by weight of diammonium phosphate (NH4H2PO4), 10.0 to 16.0 parts by weight of potassium phosphate (KH2PO4), 14 to 20 parts by weight of magnesium sulfate (MgSO4·7H2O), 0.01 to 0.03 parts by weight of copper sulfate (CuSO4·5H2O), 0.1 to 0.3 parts by weight of boron (H3BO3), 0.1 to 0.3 parts by weight of manganese sulfate (MnSO4·5H2O), 0.1 to 0.3 parts by weight of zinc sulfate (ZnSO4·7H2O), and 0.01 to 0.03 parts by weight of sodium molybdate (Na2MoO4·2H2O); characterized by comprising a high-quality culture solution. A culture medium composition for promoting growth is provided.

[0028] The culture solution composition for promoting the growth of coriander according to the present invention is preferably,

[0029] A first culture solution containing 47 to 53 parts by weight of potassium nitrate (KNO3), 67 to 73 parts by weight of calcium nitrate (Ca(NO3)2·4H2O), and 0.8 to 1.4 parts by weight of iron (Fe-EDTA); and

[0030] A second culture solution containing 47 to 53 parts by weight of potassium nitrate (KNO3), 5.0 to 9.0 parts by weight of diammonium phosphate (NH4H2PO4), 11.0 to 15.0 parts by weight of potassium phosphate (KH2PO4), 15 to 19 parts by weight of magnesium sulfate (MgSO4·7H2O), 0.015 to 0.025 parts by weight of copper sulfate (CuSO4·5H2O), 0.15 to 0.25 parts by weight of boron (H3BO3), 0.15 to 0.25 parts by weight of manganese sulfate (MnSO4·5H2O), 0.15 to 0.25 parts by weight of zinc sulfate (ZnSO4·7H2O), and 0.01 to 0.02 parts by weight of sodium molybdate (Na2MoO4·2H2O); It can be included.

[0031] The culture solution composition for promoting the growth of coriander according to the present invention is, more preferably,

[0032] A first culture solution containing 49 to 51 parts by weight of potassium nitrate (KNO3), 69 to 71 parts by weight of calcium nitrate (Ca(NO3)2·4H2O), and 0.9 to 1.2 parts by weight of iron (Fe-EDTA); and

[0033] A second It may include a culture medium.

[0034] The culture solution composition for promoting the growth of coriander according to the present invention is, most preferably,

[0035] A first culture solution containing 50.5 parts by weight of potassium nitrate (KNO3), 70.8 parts by weight of calcium nitrate (Ca(NO3)2·4H2O), and 1.15 parts by weight of iron (Fe-EDTA); and

[0036] It may include a second culture solution containing 50.5 parts by weight of potassium nitrate (KNO3), 6.9 parts by weight of diammonium phosphate (NH4H2PO4), 13.6 parts by weight of potassium phosphate (KH2PO4), 17.71 parts by weight of magnesium sulfate (MgSO4·7H2O), 0.01768 parts by weight of copper sulfate (CuSO4·5H2O), 0.20009 parts by weight of boron (H3BO3), 0.24492 parts by weight of manganese sulfate (MnSO4·5H2O), 0.15391 parts by weight of zinc sulfate (ZnSO4·7H2O), and 0.01261 ​​parts by weight of sodium molybdate (Na2MoO4·2H2O).

[0037] Based on the composition of the most desirable culture medium composition, when each component in the composition is expressed in grams per liter of volume, the culture medium composition is,

[0038] A first culture solution containing 70.8g of calcium nitrate (Ca(NO3)2·4H2O), 50.5g of potassium nitrate (KNO3), and 1.15g of iron (Fe-EDTA) per 1L volume; and

[0039] It may include a second culture solution containing 50.5g of potassium nitrate (KNO3), 6.9g of diammonium phosphate (NH4H2PO4), 13.6g of potassium phosphate (KH2PO4), 17.71g of magnesium sulfate (MgSO4·7H2O), 0.01768g of copper sulfate (CuSO4·5H2O), 0.20009g of boron (H3BO3), 0.24492g of manganese sulfate (MnSO4·5H2O), 0.15391g of zinc sulfate (ZnSO4·7H2O), and 0.01261g of sodium molybdate (Na2MoO4·2H2O) per 1L volume.

[0040] The first culture solution and the second culture solution included in the culture solution composition for promoting coriander growth can be prepared separately before use and stored in a refrigerator, and then mixed and diluted for use as needed.

[0041] When the most desirable culture solution composition is expressed as a culture solution obtained by mixing the first culture solution and the second culture solution, the culture solution composition is,

[0042] Per 1L volume, it may contain 70.8g of calcium nitrate (Ca(NO3)2·4H2O), 101g of potassium nitrate (KNO3), 1.15g of iron (Fe-EDTA), 6.9g of ammonium dihydrogen phosphate (NH4H2PO4), 13.6g of potassium phosphate (KH2PO4), 17.71g of magnesium sulfate (MgSO4·7H2O), 0.01768g of copper sulfate (CuSO4·5H2O), 0.20009g of boron (H3BO3), 0.24492g of manganese sulfate (MnSO4·5H2O), 0.15391g of zinc sulfate (ZnSO4·7H2O), and 0.01261g of sodium molybdate (Na2MoO4·2H2O). there is.

[0043] In the present invention, the electrical conductivity (EC) of the high-quality culture solution composition is 2 to 4 dS·m -1 It may be, preferably 3 to 4 dS·m -1 It may be. The electrical conductivity of the culture solution composition for promoting coriander growth is 2 dS·m -1 If it is less than this, it fails to provide sufficient inorganic nutrients to the plant, so the growth of coriander is not smooth; consequently, the number or width of leaves may be insufficient, and mineral deficiency symptoms may appear in the plant. The electrical conductivity of the culture solution composition for promoting coriander growth is 4 dS·m -1 In cases of excessively high concentrations, excessive waste of inorganic nutrients and osmotic stress in the plant may occur, which can actually hinder the growth of coriander.

[0044] As used herein, the term "electrical conductivity (EC)" refers to the ability of a substance to conduct electric current and is an indicator reflecting the concentration of ions. Electrical conductivity increases as the ion concentration in the culture medium increases, indicating the ease of current flow within the substance.

[0045] In addition, the present invention provides a method for cultivating coriander using the aforementioned coriander culture solution composition.

[0046] Specifically, the method of cultivating coriander is,

[0047] 1) The planting stage of planting coriander seedlings in a hydroponic facility;

[0048] 2) The above coriander at 150 to 250 μmol·m -2 ·s -1A first culture solution containing 45 to 55 parts by weight of potassium nitrate (KNO3), 65 to 75 parts by weight of calcium nitrate (Ca(NO3)2·4H2O), and 0.6 to 1.6 parts by weight of iron (Fe-EDTA), while maintaining a temperature of 20 to 30°C under a range of light intensity; and a second culture solution comprising 45 to 55 parts by weight of potassium nitrate (KNO3), 4.0 to 10.0 parts by weight of diammonium phosphate (NH4H2PO4), 10.0 to 16.0 parts by weight of potassium phosphate (KH2PO4), 14 to 20 parts by weight of magnesium sulfate (MgSO4·7H2O), 0.01 to 0.03 parts by weight of copper sulfate (CuSO4·5H2O), 0.1 to 0.3 parts by weight of boron (H3BO3), 0.1 to 0.3 parts by weight of manganese sulfate (MnSO4·5H2O), 0.1 to 0.3 parts by weight of zinc sulfate (ZnSO4·7H2O), and 0.01 to 0.03 parts by weight of sodium molybdate (Na2MoO4·2H2O); a culture solution composition comprising Growth stage that is applied to grow; and

[0049] 3) A step of harvesting coriander that has undergone the above growth stage may be included.

[0050] In the present invention, the planting step is the step of planting coriander seedlings in a hydroponic cultivation facility. The hydroponic cultivation facility may be an indoor crop cultivation facility called a plant factory.

[0051] Plant factories can efficiently cultivate plants indoors by controlling light sources, carbon dioxide concentration within the facility, temperature, humidity, and nutrients in the growing medium. The hydroponic facility may be a nutrient film technique (NFT) or deep flow technique (DFT) system, but is not specifically limited to these.

[0052] Thin-film hydroponic systems have a simple structure and are lightweight, making them easy to raise and cultivate crops, which results in high work efficiency. In addition, since the nutrient solution flows according to the slope of the bed, it facilitates oxygen supply to the roots and has the advantage of good responsiveness in controlling the environment of the root zone.

[0053] The deep water hydroponic system has the advantage of being able to supply the nutrient solution while maintaining uniform nutrient concentration, nutrient ion balance, and pH control, as the roots are submerged in a deep nutrient solution, and the amount of nutrient solution in the bed is large, resulting in minimal fluctuation in the root environment.

[0054] In the present invention, the growth step is a step of growing coriander while controlling light intensity, ambient temperature, etc., by applying the aforementioned coriander growth-promoting culture solution composition.

[0055] The light intensity during the growth stage is 150 to 250 μmol·m⁻¹. -2 ·s -1 It may be within the range. Maintaining light intensity within this range can promote the growth of coriander, and a light intensity of 150 μmol·m² -2 ·s -1 Low light intensities below a certain level inhibit photosynthesis, which can lead to insufficient growth of coriander, and a light intensity of 250 μmol·m² -2 ·s -1 Excessive light wasted electrical energy and partially destroyed chlorophyll in plants or inactivated internal conditions, which inhibited photosynthesis and may instead lead to problems where excellent growth of the plant cannot be induced.

[0056] During the growth stage, the cultivation temperature can be maintained at 20 to 30°C. Maintaining this temperature range allows for efficient induction of coriander growth. If the cultivation temperature is below 20°C, the coriander may experience cold stress, which becomes a limiting factor for photosynthesis and slows down its growth; if it exceeds 30°C, the degree of photosynthesis decreases, and increased respiration may inhibit growth.

[0057] In order to efficiently grow coriander in hydroponic facilities, it is desirable to cultivate it using a nutrient solution dedicated to coriander. Additionally, amino acids or vitamins that aid in the growth of coriander can be supplied; for example, vitamin B1, which promotes cell metabolism, and vitamin C, which increases stress resistance through antioxidant action, can be supplied.

[0058] In the present invention, coriander grown using the aforementioned coriander growth-promoting culture solution composition may have a tip burn rate reduced by 10 to 30% compared to coriander grown using a conventional nutrient solution, but is not limited thereto.

[0059] Tip burn, one of the most problematic physiological disorders in plant cultivation, refers to the necrosis of plant leaf tissue. When tip burn occurs, the tips of the leaves become sunken or turn brown, eventually leading to the leaves' death.

[0060] In the present invention, coriander grown using the aforementioned coriander growth-promoting culture solution composition may have a shoot length of 10 to 30% increased compared to coriander grown using a conventional nutrient solution, but is not limited thereto.

[0061] In the present invention, coriander grown using the aforementioned coriander growth-promoting culture solution composition may have a shoot fresh weight increased by 10 to 30% compared to coriander grown using a conventional nutrient solution, but is not limited thereto.

[0062] In the present invention, coriander grown using the aforementioned coriander growth-promoting culture solution composition may have a shoot dry weight that increases by 10 to 30% compared to coriander grown using a conventional nutrient solution, but is not limited thereto.

[0063] In the present invention, coriander grown using the aforementioned coriander growth-promoting culture solution composition may have a leaf area that increases by 10 to 30% compared to coriander grown using a conventional nutrient solution, but is not limited thereto.

[0064] In the present invention, the above-described culture solution composition for promoting coriander growth may have a pH range of 5 to 6.

[0065] If the pH exceeds 6, phosphate and calcium become insoluble, and the stability of iron may decrease. If the pH is below 5, symptoms of excess of specific ions may appear due to increased absorption.

[0066] According to a specific embodiment of the present invention, the inventors, in order to analyze the inorganic matter of coriander, used a temperature of 20°C, a relative humidity of 80%, white LEDs, and 150 μmol·m² -2 ·s -1 Coriander was sown and grown as seedlings in a vertical farm with a photosynthetic photon flux density (PPFD) of 16h and a photoperiod of 16h, and after transplanting, the temperature was 23℃, relative humidity 70%, white LEDs, and 250 μmol·m². -2 ·s -1Coriander was cultivated by changing the photosynthetic photon flux density (PPFD). Subsequently, the mineral content of the cultivated coriander was analyzed using the wet decomposition method, and it was confirmed that it contained 75 to 77% of the cation K, 17 to 19% of Ca, and 3 to 6% of Mg. It was confirmed that it contained 76 to 79% of the anionic phosphorus N, 12 to 16% of P, and 7 to 8% of S (see Table 1). Analysis of the mineral content of the existing nutrient solutions, Hoagland solution and Mulpuré solution, revealed a large deviation from the mineral ratio of the coriander (see Table 2 and Figure 1), suggesting that the existing nutrient solutions are not suitable for coriander cultivation. A new nutrient solution specifically for coriander was prepared based on the mineral ratio absorbed by the coriander (see Table 3). This suggests that, compared to conventional nutrient solutions, the prepared culture solution specifically designed for coriander is composed of a ratio of inorganic ions preferred by coriander, thereby inducing superior growth promotion. The coriander growth-inducing activity of the prepared culture solution and the water ash nutrient solution was compared, and the activity was evaluated based on electrical conductivity. Coriander grown using the coriander-specific culture solution showed a 10 to 30% decrease in tip burn rate and a 10 to 30% increase in leaf area compared to coriander grown using the water ash nutrient solution. Furthermore, it was confirmed that the length of the underground part of the coriander increased by 10 to 40%, and the fresh weight, dry weight, and length of the above-ground part increased by 10 to 30%, respectively. This suggests that the coriander-specific culture solution leads to superior growth compared to the conventional water ash nutrient solution (see Fig. 2). The degree of change in pH and electrical conductivity of the coriander-specific culture solution and the water ash nutrient solution was measured during the cultivation period. It was confirmed that the ash nutrient solution with electrical conductivity 1 and 2 fluctuated significantly from pH 4.0 to 6.5 during the cultivation period, whereas the cilantro-specific nutrient solution with electrical conductivity 3 and 4 had a small fluctuation range from pH 5.0 to 6.0.Regarding changes in electrical conductivity, it was confirmed that the electrical conductivity of the ash nutrient solution increased slightly, while the electrical conductivity of the cilantro-specific nutrient solution remained unchanged (see Fig. 3). This suggests that the cilantro-specific nutrient solution, which has a small range of pH change and a small change in ion concentration, is more stable in the long term and more desirable than the ash nutrient solution for cultivating cilantro that can be harvested multiple times.

[0067] Meanwhile, embodiments of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below. Furthermore, embodiments of the present invention are provided to more fully explain the present invention to those with average knowledge in the relevant technical field.

[0068] The present invention will be explained in more detail below with reference to examples.

[0069] <Example 1> Preparation of culture solution dedicated to coriander

[0070] <Example 1-1> Analysis of High-Quality Minerals

[0071] Coriander seeds sown in a sponge (Danong, Gyeonggi-do, South Korea) were subjected to a temperature of 20℃, relative humidity of 80%, white LEDs, and 150 μmol·m². -2 ·s -1 Seeds were sown in a vertical farm with a photosynthetic photon flux density (PPFD) of 16h and a photoperiod of 16h.

[0072] Two weeks after sowing, seedlings were grown in a Deep Water Fusion (DFT) system using two nutrient solutions (Hoagland solution and Mulpur solution) under conditions of pH 5.8 to 6.0 and an electrical conductivity of 1 or 2. All seedlings were grown at a temperature of 23°C, relative humidity of 70%, white LEDs, and 250 μmol·m². -2 ·s -1 It was grown in an environment with a photosynthetic photon flux density (PPFD) and a photoperiod of 16h.

[0073] Three weeks after planting, coriander was harvested, separated into above-ground and underground parts, dried in a hot air dryer for more than 72 hours, and then ground in a milling tube to produce powder samples. The inorganic content was analyzed using the wet digestion method. The content of K, S, P, Ca, Mg, Fe, Zn, and Mn was analyzed using an inductively coupled plasma spectrometer (ICP-OSE), and the content of N was analyzed via organic elemental analysis (OEA). The results are listed in Table 1.

[0074] Specifically, as a result of analyzing the mineral content of the above-ground parts of coriander, it was confirmed that it contains 75 to 77% of the cation K, 17 to 19% of Ca, and 3 to 6% of Mg. In addition, it was confirmed that it contains 76 to 79% of the anion N, 12 to 16% of P, and 7 to 8% of S.

[0075] Analysis of mineral content in the above-ground parts of coriander Cations (%) Anions (%) K Ca Mg N P S Hoagland (electrical conductivity 1) 76.83 17.19 5.97 78.6 12.82 8.58 Ash (electrical conductivity 1) 77.01 19.18 3.72 79.47 12.6 7.93 Hoagland (electrical conductivity 2) 75.95 17.77 6.28 76.87 16.03 7.1 Ash (electrical conductivity 2) 76.11 18.91 4.97 79.76 12.55 7.69

[0076] <Example 1-2> Analysis of inorganic substances in existing culture medium

[0077] The existing Hoagland culture solution was proposed in the 1938 paper by Hoagland and Arnon and is the most commonly used culture solution due to its high versatility for leafy vegetables. Hoagland stock solution A contains 236.15g of Ca(NO3)2·4H2O, 75.825g of KNO3, and 10.5275g of Fe-EDTA per 1L, and stock solution B contains 75.825g of KNO3, 28.765g of NH4H2PO4, 123.24g of MgSO4·7H2O, 0.02g of CuSO4·5H2O, 0.715g of H3BO3, 0.4525g of MnCl2·4H2O, 0.055g of ZnSO4·7H2O, and 0.005g of Na2MoO4·2H2O per 1L. A Hoagland solution with an electrical conductivity of 1 was prepared by adding 2 ml of Hoagland stock solutions A and B per 1 L, and a Hoagland solution with an electrical conductivity of 2 was prepared by adding 4 ml of Hoagland stock solutions A and B per 1 L.

[0078] The existing Mulpuré nutrient solution is a solution specifically designed for hydroponics and irrigation that passed a two-year trial at the Horticultural Experiment Station of the Rural Development Administration following thorough research on the nutritional physiology of leafy vegetables, flowers, and fruit vegetables. It is a universally used solution for the hydroponic cultivation of leafy vegetables and flowers. Mulpuré nutrient solution consists of inorganic components and an aqueous solution (water). The inorganic component composition of Mulpuré Storage Solution A is as follows: Nitrogen (N): 5.5%; Potassium (K): 4.5%; Lime (Ca): 2%; Boron (B): 0.00014%; Iron (Fe): 0.03%; Zinc (Zn): 0.0001%; ​​Molybdenum (Mo): 0.0002%. The inorganic component composition of Mulpuré Storage Solution B is as follows: Nitrogen (N): 2.5%; Phosphoric Acid (P2O5): 2.2%; Potassium (K): 5.3%; Magnesium (Mg): 1%; Manganese (Mn): 0.007%; Boron (B): 0.05%; Zinc (Zn): 0.003%; Copper (Cu): 0.0007%. An ash nutrient solution with an electrical conductivity of 1 was prepared by adding 2 ml of ash stock solutions A and B per 1 L, and an ash nutrient solution with an electrical conductivity of 2 was prepared by adding 4 ml of ash stock solutions A and B per 1 L.

[0079] Subsequently, the mineral content of Hoagland nutrient solution, a commercially available culture solution commonly used for coriander cultivation, and the nutrient solution of ash were analyzed. The content of K, S, P, Ca, Mg, Fe, Zn, and Mn was analyzed using an inductively coupled plasma spectrometer (ICP-OSE), and the content of N was analyzed through colorimetric analysis of NH4-N (ammonia nitrogen) and NO3-N (nitrate nitrogen), and the results are listed in Table 2.

[0080] Specifically, it was confirmed that the Hoagland solution contains 46 to 47% of the cation K, 12 to 13% of Ca, and 39 to 41% of Mg. It was also confirmed that it contains 70 to 75% of the anion N, 2 to 3% of P, and 4 to 6% of S.

[0081] In addition, it was confirmed that the ash nutrient solution contains 47 to 50% of the cation K, 5 to 6% of Ca, and 43 to 45% of Mg. It was also confirmed that it contains 80 to 81% of the anion N, 2 to 3% of P, and 2 to 3% of S.

[0082] This suggests that because the mineral content of existing nutrient solutions, such as Hoagland and ash solutions, differs from that of coriander, the existing solutions are inefficient for promoting coriander growth, and that it is necessary to prepare a nutrient solution with optimal ratios.

[0083] Existing analysis of nutrient solution mineral content Cations (%) Anions (%) K Ca Mg N P S Hoagland (electrical conductivity 1) 46.35 12.02 41.63 75.04 2.90 4.54 Ash (electrical conductivity 1) 47.93 6.28 45.79 82.66 2.92 2.88 Hoagland (electrical conductivity 2) 47.18 13.59 39.22 70.24 2.77 6.42 Ash (electrical conductivity 2) 50.85 5.53 43.62 81.60 2.48 2.73

[0084] <Examples 1-3> Development of a Culture Solution Dedicated to Cilantro

[0085] A culture solution specifically for coriander was prepared according to the ratio of inorganic matter absorbed by the coriander plant. The culture solution specifically for coriander is prepared by mixing the first culture solution and the second culture solution.

[0086] Based on a 100x dilution ratio, the culture solution dedicated to coriander contains 70.8g of calcium nitrate (Ca(NO3)2·4H2O), 50.5g of potassium nitrate (KNO3), and 1.15g of iron (Fe-EDTA) per 1L for the first culture solution, and 50.5g of potassium nitrate (KNO3), 6.9g of ammonium dihydrogen phosphate (NH4H2PO4), 13.6g of potassium phosphate (KH2PO4), 17.71g of magnesium sulfate (MgSO4·7H2O), 0.01768g of copper sulfate (CuSO4·5H2O), 0.20009g of boron (H3BO3), and 0.24492g of manganese sulfate (MnSO4·5H2O) per 1L for the second culture solution. It contains 0.15391g of zinc sulfate (ZnSO4·7H2O) and 0.01261g of sodium molybdate (Na2MoO4·2H2O) (see Table 3).

[0087] In order to analyze the mineral content of actual coriander and the culture solution, the mineral content of coriander was compared with that of the coriander-specific culture solution and the ash nutrient solution. As a result, it was confirmed that the coriander-specific culture solution was similar to the mineral content of coriander compared to the ash nutrient solution (see Fig. 1).

[0088] Through this, the nutrient solution for coriander provided in the present invention is composed of a ratio of inorganic ions preferred by coriander, which is very similar to the ratio of cations and anions in coriander, suggesting that the nutrient solution for coriander of the present invention can induce relatively superior coriander growth promotion compared to conventional Hoagland nutrient solution and ash nutrient solution.

[0089] Composition of a culture solution specifically for coriander 100x dilution First culture solution ingredient Content (g / L) Ca(NO₃)₂·4H₂O 70.8 KNO₃ 50.5 Fe-EDTA 1.15 2nd culture solution KNO₃ 50.5 NH₄H₂PO₄·4H₂O 6.9 KH2PO₄·4H₂O 13.6 MgSO₄·4H₂O 17.71 CuSO₄·4H₂O 0.01768 H₃BO₃ 0.20009 MnSO₄·5H₂O 0.24492 ZnSO₄·7H₂O 0.15391 Na₂MoO₄·2H₂O 0.01261

[0090] <Example 2> Evaluation of High-Quality Growth-Promoting Inducing Activity and Nutrient Solution Characteristics

[0091] <Example 2-1> Confirmation of growth-promoting inducing activity according to nutrient solution

[0092] The following experiments were performed to evaluate the growth-promoting activity of the nutrient solution dedicated to cilantro (New) and the ash nutrient solution prepared in Examples 1-3, and to evaluate the activity according to electrical conductivity EC (dS / m).

[0093] More specifically, this experiment was conducted at a temperature of 20℃, relative humidity of 80%, white LEDs, and 150 μmol·m² -2 ·s -1 Photosynthetic photon flux density (PPFD) and a photoperiod of 16h were carried out in a vertical farm.

[0094] The nutrient solution treatment conditions are ash nutrient solution (M) EC 1.0 dS·m -1 and 2.0 dS·m -1 And the developed culture solution exclusively for coriander (New) by EC level (1.0, 2.0, 3.0, 4.0 dS·m) -1Cultivated by treatment with ). At 2 and 3 weeks after planting, tip burn rate, SPAD, crown diameter, leaf area, fresh weight and dry weight of the above-ground and underground parts, above-ground length, and underground length were measured.

[0095] When comparing coriander grown using a coriander-specific nutrient solution with coriander grown using an ash nutrient solution, no significant difference was observed in SPAD and crown diameter; however, it was confirmed that the coriander grown using the coriander-specific nutrient solution had a tip burn rate that decreased by 10 to 30% compared to the coriander grown using an ash nutrient solution, and in particular, it was confirmed that the tip burn rate decreased significantly as electrical conductivity increased, and the leaf area increased by 10 to 30% (see Fig. 2a).

[0096] In addition, when comparing the underground parts of coriander grown using a coriander-specific nutrient solution with those grown using an ash nutrient solution, no significant change was observed in root fresh weight, but it was confirmed that the root length of coriander grown using the coriander-specific nutrient solution increased by 10 to 40% compared to that of coriander grown using an ash nutrient solution, and in particular, the root length was found to be longest in the coriander-specific nutrient solution with an electrical conductivity of 3 (see Fig. 2b).

[0097] In addition, when comparing the above-ground parts of coriander grown using a coriander-specific nutrient solution with those grown using an ash nutrient solution, it was confirmed that the above-ground fresh weight, above-ground dry weight, and above-ground length of the coriander grown using the coriander-specific nutrient solution increased by 10 to 30% compared to the coriander grown using the ash nutrient solution (see Fig. 2c).

[0098] Through this, it was confirmed that the nutrient solution dedicated to coriander resulted in superior growth compared to the ash nutrient solution, and the developed nutrient solution dedicated to coriander is efficient in maintaining the ratio of inorganic ions, particularly with an electrical conductivity of 2 to 4 dS·m -1 In this case, it can be confirmed that it helps with growth.

[0099] <Example 2-2> Confirmation of changes in nutrient solution characteristics

[0100] The following experiment was conducted to measure the rate of change in pH and electrical conductivity (EC) of the nutrient solution during the cultivation period.

[0101] The Grave of the Master, 23℃, relative humidity 70%, white LEDs, 250 μmol·m -2 ·s -1 It was cultivated in a vertical farm with a photosynthetic photon flux density (PPFD) and a photoperiod of 16h. The pH and EC of the nutrient solution were checked daily for 3 weeks after planting.

[0102] As a result, it was confirmed that the existing ash nutrient solution with electrical conductivity 1 and 2 gradually decreased from the initial pH of 6.5 as it progressed from the early to the late growth stage, followed by a rapid decrease to below pH 4.0, then rapidly increased back to pH 6.5, and subsequently decreased gradually. In contrast, among the cilantro-specific nutrient solutions (New), the solution with electrical conductivity 3 and 4 was found to gradually decrease from an initial pH of 6.0 to 5.0, and then gradually increased to maintain 6.0. The cilantro-specific nutrient solution with electrical conductivity 1 and 2 was found to gradually decrease from an initial pH of 6.0 to 5.0, and then increased to 7.0 (see Fig. 3a).

[0103] The pH of a nutrient solution is related to changes in the form or solubility of inorganic ions and directly affects the availability of nutrients. As pH increases, phosphate and calcium bind and become insoluble, while the stability of iron decreases. Conversely, if pH decreases, symptoms of excess specific ions appear, such as increased absorption. Therefore, maintaining an appropriate pH can extend the shelf life of the culture solution for nutrient supply and is related to the availability and ratio stability of inorganic ions within the solution.

[0104] This indicates that the nutrient solution dedicated to coriander exhibited less pH fluctuation compared to the nutrient solution for water ash. Furthermore, since the coriander-specific solution was developed based on the ratio of inorganic ions found in coriander, it is judged that the ratio of inorganic ions within the solution is more stable than that of water ash. This suggests that using a coriander-specific nutrient solution with a long-term stable ratio of inorganic ions is desirable for coriander cultivation that allows for multiple harvests.

[0105] In addition, regarding changes in electrical conductivity, the ash nutrient solution with an electrical conductivity of 1 and the citron-specific culture solution decreased slightly over time, while the electrical conductivity of the ash nutrient solution with an electrical conductivity of 2.0 increased slightly, whereas the citron-specific culture solution showed almost no change, and the electrical conductivity of the citron-specific culture solutions with electrical conductivity of 3 and 4 showed almost no change and increased slightly after more than 2 weeks (see Fig. 3b).

[0106] This suggests that the nutrient solution dedicated to coriander has higher stability in ion concentration compared to the existing ash nutrient solution, making it more suitable for coriander cultivation.

Claims

Claim 1 A first culture solution containing 45 to 55 parts by weight of potassium nitrate (KNO3), 65 to 75 parts by weight of calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), and 0.6 to 1.6 parts by weight of iron ethylenediaminetetraacetate (Fe-EDTA); and 45 to 55 parts by weight of potassium nitrate (KNO3), 4.0 to 10.0 parts by weight of ammonium dihydrogen phosphate (NH4H2PO4), 10.0 to 16.0 parts by weight of potassium phosphate (KH2PO4), 14 to 20 parts by weight of magnesium sulfate heptahydrate (MgSO4·7H2O), 0.01 to 0.03 parts by weight of copper sulfate pentahydrate (CuSO4·5H2O), 0.1 to 0.3 parts by weight of boron (H3BO3), 0.1 to 0.3 parts by weight of manganese sulfate pentahydrate (MnSO4·5H2O), 0.1 to 0.3 parts by weight of zinc sulfate heptahydrate (ZnSO4·7H2O), and 0.01 to 0.01 parts by weight of sodium molybdate dihydrate (Na2MoO4·2H2O). A culture solution composition for promoting the growth of coriander, characterized by comprising a second culture solution containing 0.03 parts by weight. Claim 2 In claim 1, the culture solution composition comprises a first culture solution containing 49 to 51 parts by weight of potassium nitrate (KNO3), 69 to 71 parts by weight of calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), and 0.9 to 1.2 parts by weight of iron ethylenediaminetetraacetate (Fe-EDTA); and 49 to 51 parts by weight of potassium nitrate (KNO3), 6.0 to 8.0 parts by weight of ammonium dihydrogen phosphate (NH4H2PO4), 12.0 to 14.0 parts by weight of potassium phosphate (KH2PO4), 16 to 18 parts by weight of magnesium sulfate heptahydrate (MgSO4·7H2O), 0.015 to 0.020 parts by weight of copper sulfate pentahydrate (CuSO4·5H2O), 0.20 to 0.25 parts by weight of boron (H3BO3), 0.20 to 0.25 parts by weight of manganese sulfate pentahydrate (MnSO4·5H2O), 0.15 to 0.20 parts by weight of zinc sulfate heptahydrate (ZnSO4·7H2O), and A culture solution composition for promoting the growth of coriander, characterized by comprising a second culture solution containing 0.01 to 0.015 parts by weight of sodium molybdate dihydrate (Na2MoO4·2H2O). Claim 3 In claim 1, the culture solution composition comprises a first culture solution containing 50.5 parts by weight of potassium nitrate (KNO3), 70.8 parts by weight of calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), and 1.15 parts by weight of iron ethylenediaminetetraacetate (Fe-EDTA); and a second culture solution containing 50.5 parts by weight of potassium nitrate (KNO3), 6.9 parts by weight of ammonium dihydrogen phosphate (NH4H2PO4), 13.6 parts by weight of potassium phosphate (KH2PO4), 17.71 parts by weight of magnesium sulfate heptahydrate (MgSO4·7H2O), 0.01768 parts by weight of copper sulfate pentahydrate (CuSO4·5H2O), 0.20009 parts by weight of boron (H3BO3), 0.24492 parts by weight of manganese sulfate pentahydrate (MnSO4·5H2O), 0.15391 parts by weight of zinc sulfate heptahydrate (ZnSO4·7H2O), and 0.01261 ​​parts by weight of sodium molybdate dihydrate (Na2MoO4·2H2O); characterized by comprising a high-quality Composition of a culture medium for growth promotion. Claim 4 A first culture solution containing 70.8g of calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), 50.5g of potassium nitrate (KNO3), and 1.15g of iron ethylenediaminetetraacetate (Fe-EDTA) per 1L volume; A culture solution for promoting the growth of coriander, characterized by comprising: a second culture solution containing 50.5g of potassium nitrate (KNO3), 6.9g of ammonium dihydrogen phosphate (NH4H2PO4), 13.6g of potassium phosphate (KH2PO4), 17.71g of magnesium sulfate heptahydrate (MgSO4·7H2O), 0.01768g of copper sulfate pentahydrate (CuSO4·5H2O), 0.20009g of boron (H3BO3), 0.24492g of manganese sulfate pentahydrate (MnSO4·5H2O), 0.15391g of zinc sulfate heptahydrate (ZnSO4·7H2O), and 0.01261g of sodium molybdate dihydrate (Na2MoO4·2H2O) per 1L volume. Composition. Claim 5 Per 1L volume: 70.8g calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), 101g potassium nitrate (KNO3), 1.15g iron ethylenediaminetetraacetate (Fe-EDTA), 6.9g ammonium diphosphate (NH4H2PO4), 13.6g potassium phosphate (KH2PO4), 17.71g magnesium sulfate heptahydrate (MgSO4·7H2O), 0.01768g copper sulfate pentahydrate (CuSO4·5H2O), 0.20009g boron (H3BO3), 0.24492g manganese sulfate pentahydrate (MnSO4·5H2O), 0.15391g zinc sulfate heptahydrate (ZnSO4·7H2O), and A culture solution composition for promoting the growth of coriander, characterized by containing 0.01261g of sodium molybdate dihydrate (Na2MoO4·2H2O). Claim 6 In claim 5, the electrical conductivity (EC) of the culture medium composition is 2 to 4 dS·m -1 A culture solution composition for promoting coriander growth, characterized by being Claim 7 A method for cultivating coriander using the culture solution composition of claim 1. Claim 8 In claim 7, the above method for cultivating coriander comprises: 1) a planting step of planting coriander seedlings in a hydroponic cultivation facility; 2) the coriander at 150 to 250 μmol·m -2 ·s -1 A method for cultivating coriander, comprising: a growth step of growing by applying the culture solution composition of claim 1 while maintaining a temperature of 20 to 30°C under a range of light intensity; and 3) a step of harvesting coriander that has undergone the growth step. Claim 9 A method for cultivating coriander according to claim 8, characterized in that the culture solution composition exhibits a pH range of 5 to 6. Claim 10 A method for cultivating coriander according to claim 8, characterized in that coriander grown using the above-mentioned culture solution composition has a tip burn rate reduced by 10 to 30% compared to coriander grown using a conventional nutrient solution. Claim 11 A method for cultivating coriander according to claim 8, characterized in that coriander grown using the above-described culture solution composition has a shoot length increased by 10 to 30% compared to coriander grown using a conventional nutrient solution. Claim 12 A method for cultivating coriander according to claim 8, characterized in that coriander grown using the above-described culture solution composition has a shoot fresh weight increased by 10 to 30% compared to coriander grown using a conventional nutrient solution. Claim 13 A method for cultivating coriander according to claim 8, characterized in that coriander grown using the above-described culture solution composition has a shoot dry weight increased by 10 to 30% compared to coriander grown using a conventional nutrient solution. Claim 14 A method for cultivating coriander according to claim 8, characterized in that coriander grown using the above-described culture solution composition has a leaf area increased by 10 to 30% compared to coriander grown using a conventional nutrient solution.

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