A functional composition that utilizes dietary fiber and ingredients that aid in intestinal movement to help relieve constipation and improve anal health problems such as hemorrhoids and piles that may arise as a result

Abstract

The present invention provides a functional composition for improving anal health by relieving constipation, comprising red grape concentrate powder, apple concentrate powder, cabbage extract powder, chicory extract powder, spinach extract powder, lactose powder, red beet concentrate powder, fermented gold kiwi powder, aloe vera gel powder, lactic acid bacteria powder, complex fermented enzyme powder, indigestible maltodextrin, and fructooligosaccharide. The functional composition according to the present invention utilizes dietary fiber and ingredients that aid in bowel movements to relieve constipation and helps improve anal health problems such as hemorrhoids and piles that may occur as a result.

Description

Technology Field

[0001] The present invention relates to a functional composition for improving anal health through the relief of constipation, and more specifically, to a functional composition that utilizes dietary fiber and ingredients that aid in bowel movements to relieve constipation and help improve anal health problems such as hemorrhoids and piles that may occur as a result. Background Technology

[0002] Constipation commonly refers to cases where the interval between bowel movements is long, but it encompasses cases where the stool is too hard to pass, the amount of stool is too small, there is no relief even after passing it, or one must take an enema or laxative to pass it. Medically, constipation is defined as having fewer than two bowel movements per week or a stool volume of 30g or less. While short-term constipation is not a significant problem, if it becomes chronic, it can lead to systemic symptoms such as abdominal distension, abdominal pain, loss of appetite, headache, skin rash, and stiff shoulders. Furthermore, it can progress to other anal diseases such as hemorrhoids or anal fissures, or to colorectal cancer due to the abnormal proliferation of intestinal bacteria.

[0003] To prevent such constipation, dietary therapy—such as reducing stress from the living environment and consuming plenty of dietary fiber—is important, but it is by no means easy to continuously practice such a lifestyle in modern society. Consequently, patients with constipation seek various medications to alleviate distressing symptoms, even if only temporarily, and it is reported that there are nearly hundreds of types of constipation treatments currently on the market.

[0004] Consequently, while various pharmaceuticals and functional foods for constipation relief are currently on the market, they suffer from the problem of temporary effects and cause various side effects depending on the type. For instance, when using irritant agents containing anthraquinone derivatives such as senna and rhubarb as herbal laxatives, side effects like abdominal pain and diarrhea occur, and concerns have been raised regarding their use during pregnancy or for continuous consumption. Furthermore, most of the diverse functional foods containing ingredients such as kelp, yacon, *Sambaeckcho* (a type of herb), *Gyeolmyeongja* (Cassia seed tea), *Donggyuja* (a type of herb tea), aloe, and seaweed have not had their efficacy sufficiently scientifically proven; as a result, they fail to resolve the fundamental problem of constipation relief, leading to a decline in product reliability. Prior art literature

[0005] Republic of Korea Published Patent No. 10-2002-0079609 The problem to be solved

[0006] The objective of the present invention is to provide a functional composition that utilizes dietary fiber and ingredients that aid in bowel movements to alleviate constipation and help improve anal health problems such as hemorrhoids and piles that may occur as a result.

[0007] The problems of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0008] To achieve the above objective, the present invention

[0009] A functional composition for improving anal health through constipation relief is provided, comprising red grape concentrate powder, apple concentrate powder, cabbage extract powder, chicory extract powder, spinach extract powder, lactose powder, red beet concentrate powder, fermented gold kiwi powder, aloe vera gel powder, lactic acid bacteria powder, complex fermented enzyme powder, indigestible maltodextrin, and fructooligosaccharide.

[0010] In addition, the composition comprises 8-12 parts by weight of red grape concentrate powder, 8-12 parts by weight of apple concentrate powder, 8-12 parts by weight of cabbage extract powder, 8-12 parts by weight of chicory extract powder, 7-9 parts by weight of spinach extract powder, 4-6 parts by weight of lactose powder, 4-6 parts by weight of red beet concentrate powder, 0.8-1.2 parts by weight of fermented gold kiwi powder, 0.08-0.12 parts by weight of aloe vera gel powder, 0.08-0.12 parts by weight of lactic acid bacteria powder, 0.8-1.2 parts by weight of complex fermented enzyme powder, 18-22 parts by weight of indigestible maltodextrin, and 18-22 parts by weight of fructooligosaccharide.

[0011] In addition, the above composition further comprises 8-12 parts by weight of a complex extract powder, and

[0012] The above-mentioned complex extract powder is characterized by being an extract powder obtained from a complex of *Jadeite*, wheat bran, and *Cloud Mushroom* mixed in a weight ratio of 1:1:1.

[0013] In addition, the above composition further comprises 8-12 parts by weight of a complex extract powder, and

[0014] The above complex extract powder is,

[0015] The method is characterized by being prepared by performing the following steps: mixing 48-52 parts by weight of a complex of *Cornus officinalis*, wheat bran, and *Cornus officinalis* in a weight ratio of 1:1:1 with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a *Monascus pilosus* strain, and fermenting at a temperature of 33-37℃; after fermentation, filtering to obtain a fermented product, mixing 8-12 parts by weight of the obtained fermented product with 43-47 parts by weight of ethanol and 43-47 parts by weight of chloroform, and extracting by ultrasonically irradiating at a temperature of 78-82℃ for 8-10 hours; and after extraction, filtering with filter paper, and then concentrating the filtrate under reduced pressure and freeze-drying.

[0016] In addition, the above composition further comprises 8-12 parts by weight of a complex extract powder, and

[0017] The above complex extract powder is,

[0018] A step of mixing 48-52 parts by weight of a complex of *Cudrania vulgaris*, wheat bran, and *Cudrania ulmoides* in a weight ratio of 1:1:1 with 48-52 parts by weight of a nutrient supply solution, and aging at a temperature of 58-62℃ for 2-4 days; a step of obtaining an aged product by filtering after aging, mixing 48-52 parts by weight of the obtained aged product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a *Monascus pilosus* strain, and fermenting at a temperature of 33-37℃; a step of obtaining a fermented product by filtering after fermentation, mixing 8-12 parts by weight of the obtained fermented product with 43-47 parts by weight of ethanol and 43-47 parts by weight of chloroform, and extracting by ultrasonic irradiation at a temperature of 78-82℃ for 8-10 hours; and after extraction, the step of filtering with filter paper, and then concentrating and freeze-drying the filtrate under reduced pressure is performed to produce, and

[0019] The above nutritional supply solution is,

[0020] A step of soaking soybeans and kelp in purified water; a step of mixing 38-42 parts by weight of a mixture of soaked soybeans and kelp in a weight ratio of 4:1 with 58-62 parts by weight of purified water, and boiling at a temperature of 88-92℃ for 2-4 hours; a step of, after boiling, placing the mixture into a grinding device, grinding it, and filtering to remove solids and obtain a filtrate; a step of adding 0.8-1.2 parts by weight of Lactobacillus plantarum to 100 parts by weight of the filtrate, and fermenting at a temperature of 33-37℃ for 22-26 hours; a step of, after fermentation, adding 0.3-0.5 parts by weight of a complex enzyme mixture of exopeptidase and endoprotease in a weight ratio of 1:1, and enzymatically treating at a temperature of 43-47℃ for 5-7 hours. The method is characterized by being prepared by performing the following steps: a step of heating to a temperature of 88-92℃ and cooling to a temperature of 18-22℃ after enzyme treatment; and a step of preparing a re-filtered liquid by re-filtering after cooling, mixing 3-7 parts by weight of calcium oxide, 3-7 parts by weight of erythritol, 1-5 parts by weight of vitamin B, 1-5 parts by weight of vitamin C, and 1-5 parts by weight of vitamin D with respect to 100 parts by weight of the re-filtered liquid, and heating to a temperature of 53-57℃ to dissolve.

[0021] In addition, the present invention

[0022] A step of soaking soybeans and kelp in purified water; a step of mixing 38-42 parts by weight of a mixture of soaked soybeans and kelp in a weight ratio of 4:1 with 58-62 parts by weight of purified water, and boiling at a temperature of 88-92℃ for 2-4 hours; a step of, after boiling, placing the mixture into a grinding device, grinding it, and filtering to remove solids and obtain a filtrate; a step of adding 0.8-1.2 parts by weight of Lactobacillus plantarum to 100 parts by weight of the filtrate, and fermenting at a temperature of 33-37℃ for 22-26 hours; a step of, after fermentation, adding 0.3-0.5 parts by weight of a complex enzyme mixture of exopeptidase and endoprotease in a weight ratio of 1:1, and enzymatically treating at a temperature of 43-47℃ for 5-7 hours. A step of preparing a nutrient supply solution comprising: a step of heating to a temperature of 88-92℃ and cooling to a temperature of 18-22℃ after enzyme treatment; and a step of preparing a re-filtered solution by re-filtering after cooling, mixing 3-7 parts by weight of calcium oxide, 3-7 parts by weight of erythritol, 1-5 parts by weight of vitamin B, 1-5 parts by weight of vitamin C, and 1-5 parts by weight of vitamin D with respect to 100 parts by weight of the re-filtered solution, and heating to a temperature of 53-57℃ to dissolve.

[0023] A step of mixing 48-52 parts by weight of a complex of *Cudrania vulgaris*, wheat bran, and *Cudrania ulmoides* in a weight ratio of 1:1:1 with 48-52 parts by weight of the nutrient supply solution and aging at a temperature of 58-62℃ for 2-4 days; a step of obtaining an aged product by filtering after aging, mixing 48-52 parts by weight of the obtained aged product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a *Monascus pilosus* strain, and fermenting at a temperature of 33-37℃; a step of obtaining a fermented product by filtering after fermentation, mixing 8-12 parts by weight of the obtained fermented product with 43-47 parts by weight of ethanol and 43-47 parts by weight of chloroform, and extracting by ultrasonic irradiation at a temperature of 78-82℃ for 8-10 hours; A step of preparing a complex extract powder comprising: a step of filtering the extract through filter paper after extraction, followed by vacuum concentration and freeze-drying the filtrate; and

[0024] The present invention provides a method for preparing a functional composition for improving anal health through constipation relief, comprising the step of mixing 8-12 parts by weight of the above-mentioned complex extract powder, 8-12 parts by weight of red grape concentrate powder, 8-12 parts by weight of apple concentrate powder, 8-12 parts by weight of cabbage extract powder, 8-12 parts by weight of chicory extract powder, 7-9 parts by weight of spinach extract powder, 4-6 parts by weight of lactose powder, 4-6 parts by weight of red beet concentrate powder, 0.8-1.2 parts by weight of fermented gold kiwi powder, 0.08-0.12 parts by weight of aloe vera gel powder, 0.08-0.12 parts by weight of lactic acid bacteria powder, 0.8-1.2 parts by weight of complex fermented enzyme powder, 18-22 parts by weight of indigestible maltodextrin, and 18-22 parts by weight of fructooligosaccharide. Effects of the invention

[0025] The functional composition according to the present invention utilizes dietary fiber and ingredients that aid in bowel movements to relieve constipation and help improve anal health problems such as hemorrhoids and piles that may occur as a result. Specific details for implementing the invention

[0026] Various embodiments are described in more detail below. The embodiments described herein may be modified in various ways. Specific embodiments may be described in detail in the detailed description. However, the specific embodiments disclosed are intended only to facilitate understanding of various embodiments. Accordingly, the technical concept is not limited by the specific embodiments disclosed, and it should be understood that it includes all equivalents or substitutions that fall within the spirit and scope of the invention.

[0027] Terms including ordinal numbers, such as first, second, first, second, etc., may be used to describe various components, but these components are not limited by the aforementioned terms. The aforementioned terms are used solely for the purpose of distinguishing one component from another.

[0028] In this specification, terms such as "comprising" or "having" are intended to indicate the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof. When a component is described as being "connected" or "connected" to another component, it should be understood that it may be directly connected to or connected to that other component, or that there may be other components in between. On the other hand, when a component is described as being "directly connected" or "directly connected" to another component, it should be understood that there are no other components in between.

[0029] Furthermore, in describing the present invention, if it is determined that a detailed description of related known functions or configurations may unnecessarily obscure the essence of the invention, such detailed description is abbreviated or omitted.

[0030] The present invention

[0031] A functional composition for improving anal health through constipation relief is provided, comprising red grape concentrate powder, apple concentrate powder, cabbage extract powder, chicory extract powder, spinach extract powder, lactose powder, red beet concentrate powder, fermented gold kiwi powder, aloe vera gel powder, lactic acid bacteria powder, complex fermented enzyme powder, indigestible maltodextrin, and fructooligosaccharide.

[0032] Hereinafter, a functional composition for improving anal health through the relief of constipation according to the present invention will be described in detail.

[0034] The above red grape concentrate powder is obtained by concentrating and pulverizing red grapes, and is a functional ingredient rich in polyphenols such as anthocyanins, procyanidins, flavonols (quercetin), and stilbenes (resveratrol), along with organic acids (tartaric acid, malic acid, etc.), sugars (glucose, fructose), and pectin-based dietary fiber derived from the skin and flesh (including seeds if necessary). Red grape polyphenols are beneficial for cardiovascular health through actions such as scavenging free radicals, inhibiting LDL oxidation, supporting endothelial function, and inhibiting platelet aggregation, while resveratrol is reported as a representative component exhibiting antioxidant, anti-inflammatory, cardiovascular protective, and anticancer activities. In a meta-analysis of randomized human controlled trials, grape-derived polyphenol products showed a tendency to improve inflammatory markers, such as a reduction in CRP, upon high-dose and long-term administration, and some whole grape products were confirmed to have a systolic blood pressure lowering effect. It is preferable that the above red grape concentrate powder contains 8-12 parts by weight, and more preferable that it contains 9-11 parts by weight, and functionality can be ensured by including it within the above content range.

[0035] The above apple concentrate powder is obtained by concentrating and powdering apples. The apple (Pyrus Malus) is the fruit of the apple tree, a deciduous tree belonging to the Rosaceae family of the Rosales order of dicotyledonous plants. In addition to palatable components such as organic acids and sugars, it contains large amounts of vitamins, dietary fiber, antioxidants, and minerals, which have an effect of preventing adult diseases such as cancer and cardiovascular diseases. It is preferable to include 8-12 parts by weight of the above apple concentrate powder, and more preferable to include 9-11 parts by weight; functionality can be ensured by including it within the above content range.

[0036] The above cabbage extract powder is extracted from cabbage. Cabbage (Brassica oleracea var. gemmifera) is an annual or biennial herb of the Brassicaceae family, belonging to the Papaverales order of dicotyledonous plants. It is known to be effective against gastric ulcers and duodenal ulcers because it is rich in lysine, an essential amino acid necessary for growth, and contains large amounts of Vitamin C, potassium, and calcium. Additionally, it is known to purify the blood, increase the body's resistance, and be effective against freckles, acne, and other skin diseases. It is preferable to include 8 to 12 parts by weight of the above cabbage extract powder, and more preferable to include 9 to 11 parts by weight, and functionality can be ensured by including it within the above content range.

[0037] The above chicory extract powder is extracted from chicory and contains fructooligosaccharides, inulin, polyphenols, and intybin as its main components. Chicory extract powder is known to have effects that help promote the proliferation of beneficial intestinal bacteria, improve intestinal health, facilitate bowel movements, provide antioxidant action, and aid in weight management and maintaining satiety. It is also known to contribute to blood sugar control and the enhancement of immunity. Furthermore, it is effective in preventing hypertension and arteriosclerosis by improving cholesterol levels and promoting vascular health. In addition, chicory contains components that have a positive effect on improving eyesight and promoting scalp health. It is preferable to include 8 to 12 parts by weight of the above chicory extract powder, and more preferable to include 9 to 11 parts by weight, and functionality can be secured by including it within the above content range.

[0038] The above spinach extract powder is extracted from spinach and is a powder-type extract prepared by extracting and concentrating active ingredients using a water-soluble solvent (water, ethanol, etc.) from the leaves of spinach (Spinacia oleracea L.) and then drying it. It is a functional ingredient containing nitrates, carotenoids (lutein, zeaxanthin, β-carotene), phenolic compounds (quercetin, kaempferol, ferulic acid, etc.), and vitamins and minerals. In randomized controlled crossover trials, the nitrates, polyphenols, and carotenoids of spinach have been confirmed to have acute beneficial effects on cardiovascular indicators, such as an increase in nitric oxide (NO) biomarkers, improvement of endothelial function, and a decrease in systolic blood pressure. Additionally, lutein and zeaxanthin are widely used as nutrients that help protect visual function by contributing to the formation of retinal macular pigment. It is preferable to include 7-9 parts by weight of the spinach extract powder, and more preferable to include 7.5-8.5 parts by weight, and functionality can be secured by including it within the above content range.

[0039] The above lactose powder is obtained by pulverizing lactose, and lactose is a disaccharide raw material obtained by purifying and crystallizing mammalian-derived lactose, having a structure in which glucose and galactose are connected by β-1,4-glycosidic bonds. It is preferable to include 4 to 6 parts by weight of the above lactose, and more preferable to include 4.5 to 5.5 parts by weight, and functionality can be secured by including it within the above content range.

[0040] The above red beet concentrate powder is produced by concentrating and pulverizing red beet (Beta vulgaris L., beetroot). It is a powdered concentrate prepared by low-temperature concentrating the juice extracted from the rootstock and then spray-freezing it. Beta-betaine pigments (betacyanins: betanin, isobetanin, etc., betaxanthin), nitrates, phenolic compounds (protocatechuic acid, etc.), glycine betaine, saponins, and carotenoids are reported as major components, among which beta-betaines and nitrates are representative functional indicator components. In a double-blind crossover study, it was confirmed that red beet-derived inorganic nitrates promote the endothelial relaxation response through the nitrate-nitrite-nitric oxide (NO) pathway in the body, thereby having the effect of temporarily reducing central (aortic) systolic blood pressure upon a single intake. In addition, betarain concentrate exhibits strong antioxidant and anti-inflammatory properties, and demonstrates tissue-protective effects through the scavenging of free radicals and the regulation of inflammatory cytokines (IL-1β, IL-6, TNF-α, etc.). Furthermore, anticancer potential, such as the inhibition of cancer cell proliferation, has been suggested in preclinical studies and some human studies, indicating high utility as a functional ingredient. It is preferable to include 4 to 6 parts by weight of the red beet concentrate powder, and more preferable to include 4.5 to 5.5 parts by weight, and functionality can be secured by including it within the above content range.

[0041] The above-mentioned fermented gold kiwi powder is produced by fermenting gold kiwis with lactic acid bacteria, etc., followed by drying and powdering. During the fermentation process, kiwi-derived dietary fiber, polyphenols, and enzymatic components such as actinidin are broken down into low molecular weights, thereby increasing physiological activity. Fermented gold kiwi powder has been reported to be effective in protecting and promoting the regeneration of the gastric mucosa, enhancing antioxidant and anti-inflammatory activities, improving functional dyspepsia and enhancing digestive function, and improving the intestinal microbial environment. It is preferable to include 0.8 to 1.2 parts by weight of the above-mentioned fermented gold kiwi powder, and it is more preferable to include 0.9 to 1.1 parts by weight; functionality can be ensured by including it within the above content range.

[0042] The above aloe vera gel powder is a powdered raw material prepared by extracting and concentrating mucilaginous polysaccharides from the inner leaf gel (inner leaf) of Aloe vera (Aloe vera, A. barbadensis Miller) and then drying (spraying, freezing, etc.). Its main components consist of high-molecular-weight mucilaginous polysaccharides such as acemannan (acetylated glucomannan) and small amounts of monosaccharides, sugar alcohols, amino acids, organic acids, and minerals. The representative polysaccharides of aloe vera gel exhibit molecular weights ranging from thousands to millions of daltons, and some high-molecular-weight fractions (e.g., aloeride) are reported to exhibit immunomodulatory activity through the activation of the NF-κB pathway and the induction of cytokine (IL-1β, TNF-α) expression in immune cells. Standardized aloe-based compositions have been confirmed to have immunosuppressive effects, such as improvements in innate and adaptive immune response indicators and antioxidant capacity. It is preferable to include 0.08-0.12 parts by weight of the aloe vera gel powder, and more preferable to include 0.09-0.11 parts by weight, and functionality can be secured by including it within the above content range.

[0043] The above lactic acid bacteria powder may include one or more types, such as Lactobacillus rhamnosus, Lactobacillus paracasei, Lactococcus lactis, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, and Bifidobacterium bifidum.

[0044] The above complex fermented enzyme powder is prepared by inoculating raw materials such as grains, fruits, and vegetables with lactic acid bacteria or strains, fermenting them, and then pulverizing them. It is preferable that the above complex fermented enzyme powder contains 4 to 6 parts by weight, and more preferable that it contains 4.5 to 5.5 parts by weight, and functionality can be secured by including it within the above content range. Specifically, using brown rice, Job's tears, barley, glutinous rice, soybeans, rice bran, wheat, oats, white beans, sorghum, flaxseed, sword bean, rye, foxtail millet, corn, foxtail millet, buckwheat, quinoa, paddy rice, lentils, black beans, chickpeas, peas, red beans, potatoes, sweet potatoes, cassava, oranges, tomatoes, apples, kiwis, bananas, dates, cabbage, green onions, grapes, watermelons, Korean melons, strawberries, persimmons, pineapples, plums, chestnuts, bean sprouts, aloe, prickly pear fruit, figs, astragalus, licorice, mugwort, ginger, kudzu root, pine needles, mint, dandelion, kale, Angelica gigas, Solomon's seal, Ophiopogon japonicus, Chrysanthemum zawadskii, Codonopsis pilosula, balloon flower root, safflower seeds, thistle, lotus root, Leonurus japonicus, daylilies, spinach, celandine, buckwheat, wild chives, butterbur, etc., steaming and sterilizing, After cooling, the cooled raw material can be inoculated with lactic acid bacteria or strains to ferment it, and then powdered to produce the product.

[0045] The above lactic acid bacteria or strains are Bacillus viscosus, Bacillus subtilis, Bacillus lentus, Bacillus ambiguus, Bacillus repens, Micrococcus conglomeratus, Micrococcus epimetheus, Micrococcus subflavescens, Micrococcus varianus, lactic acid bacteria, butyric acid bacteria, Leuconostoc mesenteroides, Lactobacillus plantarum, and Lactobacillus It could be Lactobacillus casei, Streptococcus faecalis, etc.

[0046] It is preferable to include 18-22 parts by weight of the above indigestible dextrin, and more preferable to include 19-21 parts by weight, and functionality can be ensured by including it within the above content range.

[0047] The above-mentioned fructooligosaccharide is a water-soluble dietary fiber-based oligosaccharide derived from chicory roots, etc., and is composed of fructo-oligosaccharide chains with an inulin-like structure, possessing intestinal fermentability. Fructooligosaccharides are known to contribute to intestinal health and improved bowel movements, increased satiety, improved blood sugar and lipid metabolism, and immune regulation through the proliferation of beneficial bacteria (prebiotics) and the promotion of short-chain fatty acid production. It is preferable to include 18-22 parts by weight of the above-mentioned fructooligosaccharide, and more preferable to include 19-21 parts by weight, and functionality can be secured by including it within the above content range.

[0048] The above composition preferably comprises 8-12 parts by weight of red grape concentrate powder, 8-12 parts by weight of apple concentrate powder, 8-12 parts by weight of cabbage extract powder, 8-12 parts by weight of chicory extract powder, 7-9 parts by weight of spinach extract powder, 4-6 parts by weight of lactose powder, 4-6 parts by weight of red beet concentrate powder, 0.8-1.2 parts by weight of fermented gold kiwi powder, 0.08-0.12 parts by weight of aloe vera gel powder, 0.08-0.12 parts by weight of lactic acid bacteria powder, 0.8-1.2 parts by weight of complex fermented enzyme powder, 18-22 parts by weight of indigestible maltodextrin, and 18-22 parts by weight of fructooligosaccharide.

[0049] In addition, it is preferable that the above composition further includes 8-12 parts by weight of complex extract powder, and more preferable that it further includes 9-11 parts by weight.

[0050] The above complex extract powder is applied as an extract powder obtained from a complex of *Jadeite*, wheat bran, and *Cloud Mushroom* mixed in a weight ratio of 1:1:1.

[0051] Caulerpa okamurae Weber-van Bosse is an edible seaweed belonging to the family Codiaceae, order Bryopsidae, phylum Ulvophyceae. It possesses a cystic tissue composed of a creeping abdomen and an erect part, and grows on sandy intertidal rocks, tidal pools, and subtidal rocks. The thallus is dark green and branches multiple times to form bead-shaped branches resembling grapes; the erect branches grow to a length of approximately 2–20 cm and a thickness of 2–3 mm, sometimes taking on a yellowish-brown color when mature. Caulerpa okamurae is also known as sea grapes or green caviar. It is rich in iron, calcium, vitamins, and dietary fiber, and contains antioxidant components such as polyphenols and flavonoids, leading to reports of functionalities related to antioxidant, anti-inflammatory, and metabolic improvement effects.

[0052] Wheat bran is a byproduct consisting of the outer layer, aleurone layer, and some aurone layer remaining after separating the endosperm during the milling process of wheat (Triticum spp.), and it is a food and functional ingredient containing an excess of dietary fiber based on non-starch polysaccharides (NSP). Wheat bran mainly contains arabinoxylan (AX), β-glucan, and cellulose as its primary fiber components, with AX being dominant in the proportion of NSP, followed by cellulose and β-glucan. Depending on the structural-functional characteristics of wheat bran NSP, such as viscosity, molecular weight, and the presence of bound polyphenols, health effects associated with improving blood lipids, weight management, management of type 2 diabetes, and reduction of the risk of cardiovascular disease and some cancers have been reported. In addition, wheat bran contains phenolic acids (e.g., ferulic acid, p-coumaric acid, sinapic acid, etc.), flavonoids, alkylesorcinols, phytosterols, lignin, etc., and in particular, ferulic acid is predominantly ester-bonded to arabinose residues of AX and contributes to antioxidant and anti-inflammatory effects and the formation of hemicellulose crosslinks.

[0053] The cap of the cloud mushroom (*Trametes versicolor*) is used for medicinal purposes. Known as 'Unji' in herbal medicine, it is a non-toxic mushroom herbal medicine with a sweet taste and slightly cooling properties. Unji contains important components such as amino acids, fatty acids, proteins, and polysaccharides, and is used in various anticancer treatments due to its excellent anticancer and immune-boosting effects.

[0054] In this invention, a complex extract powder derived from *Cornus officinalis*, wheat bran, and *Cornus officinalis* is applied to a functional composition, and said complex extract powder has excellent functionality for improving anal health by relieving constipation.

[0055] In addition, the above-mentioned complex extract powder is,

[0056] A method prepared by performing the following steps: mixing 48-52 parts by weight of a complex of *Cornus officinalis*, wheat bran, and *Cornus officinalis* in a weight ratio of 1:1:1 with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a *Monascus pilosus* strain, and fermenting at a temperature of 33-37°C; after fermentation, filtering to obtain a fermented product, mixing 8-12 parts by weight of the obtained fermented product with 43-47 parts by weight of ethanol and 43-47 parts by weight of chloroform, and extracting by ultrasonically irradiating at a temperature of 78-82°C for 8-10 hours; and after extraction, filtering with filter paper, and then concentrating and freeze-drying the filtrate under reduced pressure.

[0057] Furthermore, the above-mentioned complex extract powder is,

[0058] A step of mixing 48-52 parts by weight of a complex of *Cudrania vulgaris*, wheat bran, and *Cudrania ulmoides* in a weight ratio of 1:1:1 with 48-52 parts by weight of a nutrient supply solution, and aging at a temperature of 58-62℃ for 2-4 days; a step of obtaining an aged product by filtering after aging, mixing 48-52 parts by weight of the obtained aged product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a *Monascus pilosus* strain, and fermenting at a temperature of 33-37℃; a step of obtaining a fermented product by filtering after fermentation, mixing 8-12 parts by weight of the obtained fermented product with 43-47 parts by weight of ethanol and 43-47 parts by weight of chloroform, and extracting by ultrasonic irradiation at a temperature of 78-82℃ for 8-10 hours; The method used is manufactured by performing the step of, after extraction, filtering with filter paper, and then concentrating and freeze-drying the filtrate under reduced pressure.

[0059] The above-mentioned complex extract powder exhibits excellent functionality by enhancing the content of functional components contained within natural raw materials through aging with a nutrient supply solution, maximizing the functional components contained within natural raw materials through fermentation using a Monascus phyllosus strain, and significantly increasing the content of polyphenols and amino acids through ultrasonic extraction using a complex solvent containing ethanol and chloroform.

[0060] The above nutritional supply solution is,

[0061] A step of soaking soybeans and kelp in purified water; a step of mixing 38-42 parts by weight of a mixture of soaked soybeans and kelp in a weight ratio of 4:1 with 58-62 parts by weight of purified water, and boiling at a temperature of 88-92℃ for 2-4 hours; a step of, after boiling, placing the mixture into a grinding device, grinding it, and filtering to remove solids and obtain a filtrate; a step of adding 0.8-1.2 parts by weight of Lactobacillus plantarum to 100 parts by weight of the filtrate, and fermenting at a temperature of 33-37℃ for 22-26 hours; a step of, after fermentation, adding 0.3-0.5 parts by weight of a complex enzyme mixture of exopeptidase and endoprotease in a weight ratio of 1:1, and enzymatically treating at a temperature of 43-47℃ for 5-7 hours. A method prepared by performing the steps of: heating to a temperature of 88-92°C and cooling to a temperature of 18-22°C after enzyme treatment; and preparing a re-filtered liquid by re-filtering after cooling, mixing 3-7 parts by weight of calcium oxide, 3-7 parts by weight of erythritol, 1-5 parts by weight of vitamin B, 1-5 parts by weight of vitamin C, and 1-5 parts by weight of vitamin D with respect to 100 parts by weight of the re-filtered liquid, and heating to a temperature of 53-57°C to dissolve the mixture.

[0063] In addition, the present invention

[0064] A step of soaking soybeans and kelp in purified water; a step of mixing 38-42 parts by weight of a mixture of soaked soybeans and kelp in a weight ratio of 4:1 with 58-62 parts by weight of purified water, and boiling at a temperature of 88-92℃ for 2-4 hours; a step of, after boiling, placing the mixture into a grinding device, grinding it, and filtering to remove solids and obtain a filtrate; a step of adding 0.8-1.2 parts by weight of Lactobacillus plantarum to 100 parts by weight of the filtrate, and fermenting at a temperature of 33-37℃ for 22-26 hours; a step of, after fermentation, adding 0.3-0.5 parts by weight of a complex enzyme mixture of exopeptidase and endoprotease in a weight ratio of 1:1, and enzymatically treating at a temperature of 43-47℃ for 5-7 hours. A step of preparing a nutrient supply solution comprising: a step of heating to a temperature of 88-92℃ and cooling to a temperature of 18-22℃ after enzyme treatment; and a step of preparing a re-filtered solution by re-filtering after cooling, mixing 3-7 parts by weight of calcium oxide, 3-7 parts by weight of erythritol, 1-5 parts by weight of vitamin B, 1-5 parts by weight of vitamin C, and 1-5 parts by weight of vitamin D with respect to 100 parts by weight of the re-filtered solution, and heating to a temperature of 53-57℃ to dissolve.

[0065] A step of mixing 48-52 parts by weight of a complex of *Cudrania vulgaris*, wheat bran, and *Cudrania ulmoides* in a weight ratio of 1:1:1 with 48-52 parts by weight of the nutrient supply solution and aging at a temperature of 58-62℃ for 2-4 days; a step of obtaining an aged product by filtering after aging, mixing 48-52 parts by weight of the obtained aged product with 48-52 parts by weight of purified water, adding 0.8-1.2 parts by weight of a *Monascus pilosus* strain, and fermenting at a temperature of 33-37℃; a step of obtaining a fermented product by filtering after fermentation, mixing 8-12 parts by weight of the obtained fermented product with 43-47 parts by weight of ethanol and 43-47 parts by weight of chloroform, and extracting by ultrasonic irradiation at a temperature of 78-82℃ for 8-10 hours; A step of preparing a complex extract powder comprising: a step of filtering the extract through filter paper after extraction, followed by vacuum concentration and freeze-drying the filtrate; and

[0066] The present invention provides a method for preparing a functional composition for improving anal health through constipation relief, comprising the step of mixing 8-12 parts by weight of the above-mentioned complex extract powder, 8-12 parts by weight of red grape concentrate powder, 8-12 parts by weight of apple concentrate powder, 8-12 parts by weight of cabbage extract powder, 8-12 parts by weight of chicory extract powder, 7-9 parts by weight of spinach extract powder, 4-6 parts by weight of lactose powder, 4-6 parts by weight of red beet concentrate powder, 0.8-1.2 parts by weight of fermented gold kiwi powder, 0.08-0.12 parts by weight of aloe vera gel powder, 0.08-0.12 parts by weight of lactic acid bacteria powder, 0.8-1.2 parts by weight of complex fermented enzyme powder, 18-22 parts by weight of indigestible maltodextrin, and 18-22 parts by weight of fructooligosaccharide.

[0067] The functional composition according to the present invention utilizes dietary fiber and ingredients that aid in bowel movements to relieve constipation and help improve anal health problems such as hemorrhoids and piles that may occur as a result.

[0069] The present invention will be explained in more detail below by the following examples.

[0070] However, the following examples are merely illustrative of the content of the invention, and the scope of the invention is not limited by the examples and experimental examples.

[0071] <Preparation Example 1> Preparation of Complex Extract Powder-1

[0072] Atractylodes japonica, wheat bran, and cloud mushrooms were washed, freeze-dried at -50°C, and then ground. 10 parts by weight of a complex mixture of the ground Atractylodes japonica, wheat bran, and cloud mushrooms in a weight ratio of 1:1:1, 45 parts by weight of ethanol, and 45 parts by weight of chloroform were mixed, and the mixture was extracted by ultrasonography at 80°C for 9 hours. After extraction, the mixture was filtered through filter paper, and the filtrate was concentrated under reduced pressure and freeze-dried to produce a complex extract powder.

[0073] <Preparation Example 2> Preparation of Complex Extract Powder-2

[0074] Atractylodes japonica, wheat bran, and cloud mushrooms were washed, freeze-dried at -50°C, and then ground. 50 parts by weight of a complex mixture of the ground Atractylodes japonica, wheat bran, and cloud mushrooms in a weight ratio of 1:1:1 was mixed with 50 parts by weight of purified water, 1 part by weight of Monascus pilosus strain was added, and the mixture was fermented at 35°C. After fermentation, the mixture was filtered to obtain a fermented product. 10 parts by weight of the obtained fermented product, 45 parts by weight of ethanol, and 45 parts by weight of chloroform were mixed, and the mixture was extracted by ultrasonic irradiation at 80°C for 9 hours. After extraction, the mixture was filtered through filter paper, and the filtrate was concentrated under reduced pressure and freeze-dried to produce a complex extract powder.

[0075] <Preparation Example 3> Preparation of Complex Extract Powder-3

[0076] Cauliflower, wheat bran, and cloud mushroom were washed, freeze-dried at -50°C, and then ground. 50 parts by weight of a complex mixture of the ground Cauliflower, wheat bran, and cloud mushroom in a weight ratio of 1:1:1 was mixed with 50 parts by weight of a nutrient supply solution, and the mixture was aged at 60°C for 3 days. After aging, the mixture was filtered to obtain an aged product. 50 parts by weight of the obtained aged product and 50 parts by weight of purified water were mixed, 1 part by weight of Monascus pilosus strain was added, and the mixture was fermented at 35°C. After fermentation, the mixture was filtered to obtain a fermented product. 10 parts by weight of the obtained fermented product, 45 parts by weight of ethanol, and 45 parts by weight of chloroform were mixed, and the mixture was extracted by ultrasonic irradiation at 80°C for 9 hours. After extraction, the mixture was filtered through filter paper, and the filtrate was concentrated under reduced pressure and freeze-dried to produce a complex extract powder.

[0077] <Experimental Example 1> Analysis of Functional Components

[0078] To analyze the polyphenol content and amino acid content of the complex extract powder prepared in Preparation Examples 1-3 above, high-speed liquid chromatography (HPLC) analysis and analysis using an amino acid analyzer (Amino acid analyzer biochrom 30, Biochrom, UK) were performed, and the results are shown in Table 1 below.

[0079] Total polyphenol content (mg / g) Total amino acid content (mg / kg) Essential amino acid content (mg / kg) Preparation Example 1 169.53 24768.22 6041.23 Preparation Example 2 254.32 30467.23 8999.34 Preparation Example 3 407.54 39765.61 13237.53

[0080] As shown in Table 1 above, it was confirmed that the complex extract powder according to the present invention has a high total polyphenol content, total amino acid content, and essential amino acid content.

[0081] <Experimental Example 2> Analysis of Antioxidant Functionality

[0082] The antioxidant activity of the complex extract powder prepared in Preparation Examples 1-3 above was measured as DPPH (2,2-Diphenyl-1-picryl-hydrazyl) radical scavenging activity. The DPPH radical scavenging activity was determined by referring to the method of Mensor et al. 20 µl of 0.2 mM DPPH ethanol solution was added to each sample and stirred for 1 minute, then reacted in a 25°C incubator for 10 minutes, and the absorbance was measured at 517 nm using an ELISA reader. The DPPH radical scavenging activity was expressed as a percentage according to Formula 1 below, and the results are shown in Table 2 below.

[0083] <Equation 1>

[0084]

[0085] Sample name Antioxidant effect (DPPH scavenging activity, %) Preparation Example 1 55.8 Preparation Example 2 60.1 Preparation Example 3 67.9

[0086] A high value of DPPH radical scavenging activity indicates a high antioxidant effect, and as shown in Table 2 above, the complex extract powder according to the present invention was measured to have a high DPPH radical scavenging activity value, and it can be confirmed that it has excellent antioxidant functionality.

[0087] <Experimental Example 3> Analysis of Anti-inflammatory Functionality

[0088] Using the complex extract powder prepared in Preparation Examples 1-3 above as a sample, the effect on inhibiting the production of NO (Nitric Oxide), which is known to play a significant role in inducing inflammation, was confirmed. While general NO formation plays an important role in killing bacteria or eliminating tumors, NO generated by iNOS under inflammatory conditions is known to not only promote inflammatory responses such as vascular permeability and edema but also exacerbate inflammation by promoting the biosynthesis of inflammatory mediators. Raw 264.7 cells were [subject to] 5×10⁶ using DMEM medium supplemented with 10% [subject to] 5After adjusting to cell / well, the cells were inoculated into a 24-well plate. After treating with the sample and incubating for 30 minutes, LPS (Lipopolysaccharide, 1 μg / ml) was added and incubated for 24 hours. The amount of NO produced was calculated using Equation 2 below based on measurements taken with an NO detection kit (iNtRON), and the control group was the experimental group treated with LPS alone. The results of the NO production inhibition rate are shown in Table 3 below.

[0089] <Equation 2>

[0090]

[0091] Sample name NO production inhibition rate (%) Preparation Example 1 64.8 Preparation Example 2 68.5 Preparation Example 3 75.1

[0092] As shown in Table 3 above, the complex extract powder according to the present invention exhibited a very high NO production inhibition rate and demonstrated excellent anti-inflammatory effects.

[0093] <Example 1> Preparation of a Functional Composition-1

[0094] A functional composition was prepared by mixing 10 parts by weight of red grape concentrate powder, 10 parts by weight of apple concentrate powder, 10 parts by weight of cabbage extract powder, 10 parts by weight of chicory extract powder, 7.8 parts by weight of spinach extract powder, 5 parts by weight of lactose powder, 5 parts by weight of red beet concentrate powder, 1 part by weight of fermented gold kiwi powder, 0.1 parts by weight of aloe vera gel powder, 0.1 parts by weight of lactic acid bacteria powder, 1 part by weight of complex fermented enzyme powder, 20 parts by weight of indigestible maltodextrin, and 20 parts by weight of fructooligosaccharide.

[0095] <Example 2> Preparation of Functional Composition-2

[0096] A functional composition was prepared by adding 10 parts by weight of the complex extract powder prepared in Preparation Example 3 to the functional composition of Example 1 and mixing.

[0097] <Experimental Example 4> Analysis of Body Fat Reduction Functionality

[0098] The body fat reduction functionality of the functional compositions prepared in the above Examples 1 and 2 was confirmed through the following experiment.

[0099] <Experimental Example 4> Analysis of Constipation Relief Effect

[0100] To confirm the constipation relief effect of the compositions prepared in the above Examples 1 and 2, the following experiment was performed.

[0101] For the experiment, 4-week-old male Sprague-Dawley rats (Central Lab animal, Seoul, Korea) were raised for 2 weeks in an environment-controlled rearing room (temperature 20℃, humidity 55%, 12-hour dark / light cycle) on pellet-type solid feed (Jeil animal feed Co., Daejeon, Korea) until they reached an average body weight of approximately 210 g. 6-week-old rats were then divided into 4 groups of 12 to ensure similar average body weights. A normal group was fed a diet, and a negative control group was fed a diet after inducing constipation by administering loperamide (loperamide, Sigma, St. Louis, MO, USA) at a level of 0.03%. Experimental group 1 was fed a diet along with 10 g of the composition of Example 1 after inducing constipation by administering loperamide (loperamide, Sigma, St. Louis, MO, USA) at a level of 0.03%, and experimental group 2 was fed a diet along with 10 g of the composition of Example 2 after inducing constipation by administering loperamide (loperamide, Sigma, St. Louis, MO, USA) at a level of 0.03%. The diet consisted of 100 g of carbohydrates (65%), protein (20%), and fat (15%), and the feeding period was 5 weeks.

[0102] It has been reported that loperamide, when administered continuously for a certain period, causes constipation by increasing the duration of bowel movements and decreasing colonic peristalsis, thereby impeding the movement of colonic contents by thinning the mucus within the colonic duct, and that the symptoms are similar to those of constipation in humans (Cepinskas G. 1993. Adaptive cytoprotection in the small intestine: Role of mucus. Am J Physiol 264:921-927).

[0103] 1. Measurement of gastrointestinal (GI) transit time

[0104] We aimed to measure the intestinal transit time (GI transit time) of the intestinal contents. Four weeks after the administration of the experimental diet, the experimental animals were fasted for 12 hours. Then, 10 g of a diet mixed with 0.5% carmine red (Sigma) as an intestinal transit marker was fed first and consumed completely, after which the experimental diet was provided at the usual time. After feeding the carmine red mixed diet, the appearance of the red marker in the stool was checked at 30-minute intervals, and the intestinal transit time was defined as the interval from the time the labeled diet was fed until the first appearance of the marker in the stool. The results are shown in Table 4 below.

[0105] GI transmit time (min) Normal group 851.6 Negative control group 935.7 Experimental group 1 878.7 Experimental group 2 852.3

[0106] As shown in Table 4 above, it was confirmed that the intestinal transit time was significantly reduced compared to the negative control group when the composition according to the present invention was applied.

[0107] 2. Measurement of fecal volume and moisture content

[0108] Feces excreted during the 24 hours prior to sacrificing the experimental animals were collected twice a day, and the weight of the feces was measured immediately. The collected feces were dried at 105°C until a constant weight was reached, and the dry weight was measured. The difference in weight before and after drying was defined as the moisture content of the feces, and the results of measuring the amount of feces are shown in Table 5 below.

[0109] Fecal volume (g / day) Normal group 2.83 Negative control group 1.25 Experimental group 1 2.07 Experimental group 2 2.53

[0110] As shown in Table 5 above, it was confirmed that the amount of feces was improved compared to the negative control group when the composition according to the present invention was applied.

Claims

Claim 1 It comprises 8-12 parts by weight of red grape concentrate powder, 8-12 parts by weight of apple concentrate powder, 8-12 parts by weight of cabbage extract powder, 8-12 parts by weight of chicory extract powder, 7-9 parts by weight of spinach extract powder, 4-6 parts by weight of lactose powder, 4-6 parts by weight of red beet concentrate powder, 0.8-1.2 parts by weight of fermented gold kiwi powder, 0.08-0.12 parts by weight of aloe vera gel powder, 0.08-0.12 parts by weight of lactic acid bacteria powder, 0.8-1.2 parts by weight of complex fermented enzyme powder, 18-22 parts by weight of indigestible maltodextrin, 8-12 parts by weight of complex extract powder, and 18-22 parts by weight of fructooligosaccharide, wherein the complex extract powder is a mixture of *Cornus officinalis*, wheat bran, and *Cornus officinalis* in a weight ratio of 1:1:

1. A functional composition for improving anal health through constipation relief, characterized by being an extract powder derived from a complex. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 delete

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