Composition with anti-oxidation and moisturizing efficacy for skin care and preparation method thereof

Abstract

The present disclosure relates to an antioxidant or skin moisturizing composition comprising a complex extract of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves. The present disclosure also provides a method for preparing a composition, comprising the steps of: grinding camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves; adding an extraction solvent to the ground powder; and extracting by heating the mixture of the powder and the extraction solvent at 50 to 150°C for 5 to 60 minutes. The composition exhibits excellent antioxidant and skin moisturizing effects due to the high concentration of metabolites within the natural products.

Description

Technology Field

[0001] The present disclosure relates to an antioxidant or skin moisturizing composition comprising a complex extract of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, and a method for preparing the same. Background Technology

[0002] Free radicals, which are excess oxygen harmful to the body, are generated during processes such as energy metabolism. These free radicals attack normal cells in the body and oxidize them; in this context, cellular oxidation signifies cellular aging. In other words, free radicals act as a cause of human aging and various diseases. Antioxidant activity refers to the inhibition of such cellular oxidation, and by eliminating free radicals from the body, it is possible to prevent cellular oxidation—that is, cellular aging.

[0003] Meanwhile, the skin plays a role in protecting the body from external stimuli and maintaining homeostasis by inhibiting moisture evaporation from within. Therefore, proper skin hydration is essential for the skin's immune and protective functions to function effectively. To maintain healthy skin, most cosmetics fundamentally include moisturizing functions. While polyhydric alcohols or polysaccharides are the most commonly used moisturizing ingredients in cosmetics, they have disadvantages. Since their hydrophilic hydroxyl groups absorb moisture from the outside to retain epidermal moisture upon application, the moisturizing effect may vary depending on external conditions. Furthermore, they fail to maintain a continuous moisturizing effect, cause a sticky sensation, affect product stability when contained in large quantities, and, in the case of expensive polysaccharides, reduce price competitiveness.

[0004] Accordingly, the present invention aims to provide a natural plant-derived skin care composition that protects and maintains the skin's health through the antioxidant effect of a complex extract with increased metabolites within the natural product and the moisturizing effect through the promotion of the expression of moisture-supplying factors within the skin. Prior art literature

[0005] (Patent Document 0001) KR 10-2267058 B (Patent Document 0002) KR 10-2207995 B The problem to be solved

[0006] The object of the present disclosure is to provide a composition comprising a complex extract of natural products, which has an antioxidant function and a skin moisturizing effect.

[0007] In addition, the object of the present disclosure is to provide a method for preparing the above-mentioned antioxidant or skin moisturizing composition.

[0008] In addition, the object of the present disclosure is to provide a cosmetic composition comprising the above-mentioned antioxidant or skin moisturizing composition.

[0009] In addition, the object of the present disclosure is to provide a sanitary product comprising the above-mentioned antioxidant or skin moisturizing composition. means of solving the problem

[0010] To achieve the above objective, the present disclosure provides an antioxidant or skin moisturizing composition comprising a complex extract of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves.

[0011] In addition, the present disclosure provides an antioxidant or skin moisturizing composition comprising camphor leaves : bay leaves : bitter melon fruit : mugwort leaves in a weight ratio of 1-10 : 1-10 : 1-10 : 1-10 based on the dry weight of each natural product used in the extraction.

[0012] In addition, the present disclosure provides an antioxidant or skin moisturizing composition having a weight ratio of 1-3 : 1-3 : 1-3 : 1-3.

[0013] In addition, the present disclosure provides an antioxidant or skin moisturizing composition having a weight ratio of 2-3 : 1 : 1 : 2-3.

[0014] In addition, the present disclosure provides an antioxidant or skin moisturizing composition in which the content of the complex extract is 0.0001 to 90% based on the total weight of the composition.

[0015] In addition, the present disclosure provides an antioxidant or skin moisturizing composition in which the content of the complex extract is 0.001 to 10% based on the total weight of the composition.

[0016] In addition, the present disclosure provides an antioxidant or skin moisturizing composition in which one or more of the above camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves are roasted before being extracted with an extraction solvent.

[0017] The present disclosure also provides a composition in which the complex extract is extracted with one or more extraction solvents selected from water, purified water, and distilled water.

[0018] The present disclosure also provides a method for preparing the above composition, the method comprising the steps of: grinding camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves; adding an extraction solvent to the ground powder; and extracting by heating the mixture of the powder and the extraction solvent at 50 to 150°C for 5 to 60 minutes.

[0019] In addition, the present disclosure provides a method further comprising the step of roasting one or more of the above-mentioned camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves before extracting them with an extraction solvent.

[0020] In addition, the present disclosure provides a method in which the roasting treatment comprises stirring continuously for 10 to 60 minutes at 100 to 300°C.

[0021] The present disclosure also provides a method in which the extraction solvent is one or more selected from water, purified water, and distilled water.

[0022] In addition, the present disclosure provides a cosmetic composition comprising the above-mentioned antioxidant or skin moisturizing composition.

[0023] The present disclosure also provides a cosmetic composition comprising one or more of an antioxidant, a preservative, an absorbent, an exfoliant, a thickener, a sunscreen formulation, an emulsifier, a surfactant, a fragrance, a deodorizer, a stabilizer, a skin protectant, and a pH adjuster.

[0024] In addition, the present disclosure provides a cosmetic composition in which the cosmetic composition is selected from the group consisting of a skincare cosmetic composition, a makeup cosmetic composition, a haircare cosmetic composition, and a body care cosmetic composition.

[0025] In addition, the present disclosure provides a sanitary product comprising the above-mentioned antioxidant or skin moisturizing composition.

[0026] In addition, the present disclosure provides a sanitary product selected from the group consisting of wet wipes, tissues, diapers, sanitary pads, sanitary wipes, moist wipes, cleaning wipes, facial wipes, skincare wipes, and antiperspirant wipes. Effects of the invention

[0027] The antioxidant or skin moisturizing composition comprising the complex extract of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves of the present disclosure has a higher content of polyphenols and flavonoids compared to compositions comprising each natural substance as a single extract. In the complex extract of the present disclosure, the extracts of each plant act synergistically to effectively inhibit the activity of free radicals and exhibit high inhibitory activity against hyaluronic acid degrading enzymes. Accordingly, the complex extract of the present disclosure shows excellent effects for antioxidant and skin moisturization. Brief explanation of the drawing

[0028] Figure 1 is a graph showing the content of total polyphenol compounds of a single extract as a comparative example. Figure 2 is a graph showing the total flavonoid compound content of a single extract as a comparative example. Figure 3 is a graph showing the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability of the complex extract of the present disclosure. Figure 4 is a graph measuring the ABTS (2,2-azino-bis-3-ethylbenzo-thiazoline-6-sulfonate) radical scavenging activity of the complex extract of the present disclosure. Figure 5 is a graph showing the hyaluronidase inhibitory activity of the complex extract of the present disclosure. Specific details for implementing the invention

[0029] The embodiments or aspects of the present disclosure are illustrative for the purpose of explaining the technical concept of the present disclosure. The scope of rights according to the present disclosure is not limited to the embodiments or aspects presented below or the specific descriptions thereof.

[0030] All technical and scientific terms used in this disclosure, unless otherwise defined, have the meaning generally understood by those skilled in the art to which this disclosure pertains. All terms used in this disclosure are selected for the purpose of further clarifying this disclosure and are not selected to limit the scope of the rights under this disclosure.

[0031] The term "extract" as used in this disclosure refers to an active ingredient that can be isolated from a natural product, prepared by an extraction process using water, an organic solvent, or a mixture thereof, and is commonly understood in the art as a crude extract; however, in a broader sense, it includes all forms of extracts obtained by freeze-drying or vacuum concentration, dried powder, or formulations using the same, and also includes fractions obtained by additionally fractionating the extract. For example, a complex extract comprising camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves includes not only the extract obtained using an extraction solvent but also the extract obtained by additionally applying a purification process thereto.

[0032] Natural materials included in the extract of the present disclosure are used for extraction in a dried state. For example, camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves are extracted by mixing with an extraction solvent in a dried state.

[0033] In the composition of the present disclosure, when an extract is obtained by treating with an extraction solvent, a polar solvent or a non-polar solvent may be used. For example, polar solvents include water, C1 to C6 lower alcohols (specifically methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol, or ethylene glycol), acetic acid, dimethylformamide (DMFO), and dimethylsulfoxide (DMSO), but are not limited thereto. For example, non-polar solvents include, but are not limited to, acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkanes, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, and tetrahydrofuran (THF).

[0034] The term “single extract” as used in the present disclosure is an extract obtained from one type of natural product, and may be, for example, a single extract of camphor leaves, a single extract of bay leaves, a single extract of bitter melon fruit, and a single extract of mugwort leaves in the present disclosure.

[0035] As used in the present disclosure, "complex extract" refers to an extract obtained from two or more natural substances. The "complex extract" may be prepared by mixing two or more natural substances and extracting them together, or by preparing each single extract and then mixing the single extracts. For example, in the present disclosure, the complex extract may be prepared by mixing and extracting dried camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves, or by preparing a single extract of camphor leaves, a single extract of bay leaves, a single extract of bitter melon fruit, and a single extract of mugwort leaves, respectively, and then mixing them.

[0036] The term "roasting" as used in the present disclosure means cooking medicinal herbs, grains, tea leaves, etc. by applying heat without additives, and may refer to dry heat roasting.

[0037] Expressions such as “comprising,” “comprising,” “having,” etc. used in this disclosure should be understood as open-ended terms implying the possibility of including other embodiments, unless otherwise stated in the phrase or sentence containing such expressions.

[0038] Expressions such as "composed only of" a component used in this disclosure should be understood as closed-ended terms that exclude the possibility of including other components in addition to the component.

[0039] Unless otherwise stated, singular expressions described in this disclosure may include a plural meaning, and this applies likewise to singular expressions described in the claims.

[0040] In one aspect of the present disclosure, the term “about” is used with the intent to include manufacturing process errors included in specific figures or slight numerical adjustments falling within the scope of the technical concept of the present disclosure. For example, the term “about” means a range of ± 10% of the value it designates, ± 5% in one aspect, and ± 2% in another aspect. In the field of the present disclosure, unless the value is specifically stated to require a narrower range, this level of approximation is appropriate.

[0041] Hereinafter, embodiments of the present disclosure will be described in detail.

[0042] One embodiment of the present disclosure is an antioxidant or skin moisturizing composition comprising a complex extract of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves.

[0043] The camphor tree (Cinnamomum camphora) derives its name from the green stems of its young trunks and is also known as *jangnoemok* or *jangsu*; it thrives in deep, fertile soil or shady areas. Camphor is an oil obtained by steam distilling the wood, branches, leaves, and roots, and it is used as a raw material for making fragrances, insecticides, and cardiac stimulants. It contains flavonoid compounds such as 5,7-dimethoxy-3'4'-methylenedioxyflavan-3-ol and 4'-hydroxy-5,7,3'-trimethoxyflavan-3-ol. The leaves (*jangsuyeop*) are harvested year-round and used either fresh or dried; they are known to have efficacy in pain relief, insecticidal action, and fungicidal properties.

[0044] The laurel (Laurus nobilis) is native to the Mediterranean coast, and it is said that laurel wreaths were made from its leafy branches and used in the Pythian Games to offer sacrifices to the god Apollo. The dried leaves are called bay leaves and are used in cooking or tea due to their pleasant aroma, while in folk medicine, the fruit and leaves are used as a stomach tonic or as a remedy for boils.

[0045] Bitter melon (*Momordica Charantia*) has traditionally been widely used for medicinal purposes in various East Asian countries and the Caribbean. Extracts from its leaves and fruits are taken to treat stomach pain, fever, arthritis, diabetes, hypertension, cancer, and infectious diseases. The fruit is finely chopped, dried, and stored for medicinal use, where it is particularly effective in lowering blood sugar. The red and yellow fruits are elongated oval-shaped with numerous wart-like protrusions and possess a characteristic bitter taste, which is attributed to an alkaloid called momordicin. The fruit contains vitamins C, B1, B2, B3, B5, B9, and A, as well as calcium, iron, magnesium, zinc, and selenium.

[0046] Mugwort (Artemisia capillaris) is a woody perennial herb that reproduces via rhizomes or seeds. The young shoots are edible, and the entire plant is used as an anti-inflammatory diuretic or for jaundice. Pharmacological effects reported for Injinho, the dried above-ground medicinal herb, include promoting bile secretion, protecting liver function, regenerating liver cells, lipid decomposition, coronary artery dilation, hypotensive effects, antipyretic, diuretic, and antimicrobial activities. The leaves contain flavonoids such as quercetin 3-glucoside and rutin.

[0047] The above complex extract may be prepared by mixing and extracting camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves, or it may be prepared by preparing a single extract of camphor leaves, a single extract of bay leaves, a single extract of bitter melon fruit, and a single extract of mugwort leaves, respectively, and then mixing them.

[0048] A composition comprising a complex extract containing all of the above-mentioned camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves exhibits significantly higher antioxidant and skin moisturizing effects compared to each individual extract. Generally, when the total content of the extracts in the composition is the same based on the total weight of the composition, the evaluation indicators for the antioxidant and skin moisturizing effects of the complex extract are expected to be similar to the average values ​​of the evaluation indicators for the antioxidant and skin moisturizing effects of each individual extract; however, the evaluation indicators for the antioxidant and skin moisturizing effects of the complex extract of the present disclosure are significantly higher than the average values ​​of each individual extract, showing a synergistic effect that exceeds the additive effect.

[0049] The antioxidant effect of the above extract can be measured by conventional methods generally performed in the industry, such as DPPH analysis, ABTS analysis, FRAP analysis, ORAC analysis, measurement of polyphenol content, and measurement of flavonoid content.

[0050] In one embodiment, the average value of the DPPH radical scavenging activity of each of the above single extracts is 69.1%, whereas in the case of a complex extract containing camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, the DPPH radical scavenging activity was found to be 76.4 to 93.1% based on the total weight of the composition, even though the total amount of natural products contained is the same as that of the single extracts, which is significantly higher than the expected average value. Therefore, it was confirmed that the complex extract of the present disclosure has a synergistic effect in terms of DPPH antioxidant activity (Fig. 3).

[0051] In one embodiment, the average value of the ABTS radical scavenging activity of each of the above single extracts is 47.4%, whereas in the case of a complex extract containing camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, the ABTS radical scavenging activity was found to be 55.7 to 77.1% based on the total weight of the composition, even though the total amount of natural products contained is the same as that of the single extracts, which is significantly higher than the expected average value. Therefore, it was confirmed that the complex extract of the present disclosure has a synergistic effect in terms of ABTS antioxidant activity (Fig. 4).

[0052] A composition containing a complex extract including all of the above-mentioned camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves has a higher skin moisturizing effect compared to each individual extract. The moisturizing effect of the above extract can be measured by conventional methods generally performed in the industry, such as evaluation of hyaluronidase inhibitory activity, measurement of skin electrical capacitance, measurement of electrical capacitance based on impedance, and measurement of electrical conductivity.

[0053] In one embodiment, the average value of the hyaluronidase removal rate of each of the above single extracts is 5.3%, whereas in the case of a complex extract containing all of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, the hyaluronidase removal rate is 9.9 to 17.0% based on the total weight of the composition, even though the total amount of natural products contained is the same as that of the single extracts, which is significantly higher than the expected average value. Therefore, it was confirmed that the complex extract of the present disclosure has a synergistic effect in terms of hyaluronidase inhibitory activity (Fig. 5).

[0054] In one embodiment, the camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves of the complex extract are extracted by mixing them with an extraction solvent in a dried state.

[0055] In one embodiment, the complex extract in the composition may contain camphor leaves : bay leaves : bitter melon fruit : mugwort leaves in a weight ratio of 1-10 : 1-10 : 1-10 : 1-10 based on the dry weight of each natural product used for extraction.

[0056] The weight ratio based on the above dry weight may represent the weight ratio of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves contained in the extract when dried camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves are mixed and extracted in the same weight of the same solvent. In addition, the above weight ratio may be maintained even if other components are added to the composition or the total weight of the composition changes.

[0057] In one embodiment, the weight ratio may be: camphor leaves 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, 10 or less, 9 or less, 8 or less, 7 or less, or 6 or less; bay leaves 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, 10 or less, 9 or less, 8 or less, 7 or less, or 6 or less; bitter melon fruit 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, 10 or less, 9 or less, 8 or less, 7 or less, or 6 or less; mugwort leaves 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, 10 or less, 9 or less, 8 or less, 7 or less, or 6 or less. In another embodiment, the weight ratio may be 1-3 : 1-3 : 1-3 : 1-3, and in yet another embodiment, the weight ratio may be 2-3 : 1 : 1 : 2-3.

[0058] In one embodiment, the composition may contain camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves of the complex extract in equal weight ratios.

[0059] In one embodiment, the weight ratio of the camphor leaves and mugwort leaves in the complex extract based on the total weight of the composition may be higher than that of the bay leaves and bitter melon fruit. When the weight ratio of the camphor leaves and mugwort leaves in the complex extract based on the total weight of the composition is higher than that of the bay leaves and bitter melon fruit, the DPPH radical scavenging activity, ABTS radical scavenging activity, and hyaluronidase inhibitory activity are significantly increased compared to the case where camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves are included in equal dry weights.

[0060] In one embodiment, the content of the complex extract may be 0.0001 to 90% based on the total weight of the composition.

[0061] In one embodiment, the content of the complex extract may be 0.0001% or more, 0.0005% or more, 0.001% or more, 0.005% or more, 0.01% or more, 0.05% or more, 0.1% or more, 0.5% or more, 1% or more, 5% or more, 10% or more, 15% or more, 20% or more, 25% or more, 30% or more, 35% or more, 40% or more, 45% or more, 90% or less, 85% or less, 80% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, or 50% or less, based on the total weight of the composition.

[0062] In another embodiment, the content of the complex extract may be 0.001 to 10% based on the total weight of the composition.

[0063] In another embodiment, one or more of the above camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves may be roasted. When one or more of the above camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves are roasted, the content of natural polyphenolic compounds and flavonoid compounds, which are metabolites, increases compared to a complex extract that is not roasted, and the DPPH antioxidant activity, ABTS radical scavenging ability, and hyaluronidase inhibitory activity further increase.

[0064] In one embodiment, the complex extract may be extracted with a polar solvent or a non-polar solvent.

[0065] In one embodiment, the polar solvent is water, purified water, distilled water, C1 to C6 lower alcohol (specifically methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol), acetic acid, dimethylformamide (DMFO, dimethyl-formamide), and dimethylsulfoxide (DMSO, dimethylsulfoxide), but is not limited thereto.

[0066] In one embodiment, the non-polar solvent includes, but is not limited to, acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkanes, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, and tetrahydrofuran (THF).

[0067] In one embodiment, the complex extract may be extracted with one or more extraction solvents selected from water, purified water, and distilled water.

[0068] In one embodiment, the amount of solvent used for extraction may vary depending on the solvent, but the complex extract may be extracted with an extraction solvent in an amount of 10 to 30 times the dry weight of the natural product. For example, the extraction solvent may be added in an amount of 10 times or more, 11 times or more, 12 times or more, 13 times or more, 14 times or more, 15 times or more, 16 times or more, 17 times or more, 18 times or more, 19 times or more, 19 times or more, 20 times or more, 30 times or less, 29 times or less, 28 times or less, 27 times or less, 26 times or less, 25 times or less, 24 times or less, 23 times or less, 22 times or less, or 21 times or less of the dry weight of the natural product.

[0069] One embodiment of the present disclosure is a method for preparing the above-mentioned antioxidant or skin moisturizing composition, the method comprising the steps of: grinding camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves; adding an extraction solvent to the ground powder; and extracting by heating the mixture of the powder and the extraction solvent at 50 to 150°C for 5 to 60 minutes.

[0070] In one embodiment, the step of grinding the camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves may involve mixing the camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves and grinding them together, or grinding each of them separately.

[0071] In one embodiment, the step of adding an extraction solvent to the ground powder may involve adding an extraction solvent to the mixed powder to prepare a mixed powder solution, or adding an extraction solvent separately to each powder to prepare a single powder solution.

[0072] In one embodiment, the extraction solvent may be a polar solvent or a non-polar solvent.

[0073] In one embodiment, the polar solvent is water, purified water, distilled water, C1 to C6 lower alcohol (specifically methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol), acetic acid, dimethylformamide (DMFO, dimethyl-formamide), and dimethylsulfoxide (DMSO, dimethylsulfoxide), but is not limited thereto.

[0074] In one embodiment, the non-polar solvent includes, but is not limited to, acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkanes, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, and tetrahydrofuran (THF).

[0075] In one embodiment, the extraction solvent may be one or more selected from water, purified water, and distilled water.

[0076] In one embodiment, the amount of solvent used for extraction may vary depending on the solvent, but the extraction step may be performed by adding an extraction solvent in an amount of 10 to 30 times the dry weight of the natural product. For example, the extraction solvent may be added in an amount of 10 times or more, 11 times or more, 12 times or more, 13 times or more, 14 times or more, 15 times or more, 16 times or more, 17 times or more, 18 times or more, 19 times or more, 19 times or more, 20 times or more, 30 times or less, 29 times or less, 28 times or less, 27 times or less, 26 times or less, 25 times or less, 24 times or less, 23 times or less, 22 times or less, or 21 times or less of the dry weight of the natural product.

[0077] In one embodiment, the extraction step may be to extract a mixed powder solution of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves, or to extract a single powder solution of each of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves.

[0078] In one embodiment, the extraction step may be performed at a temperature of 50°C or higher, 55°C or higher, 60°C or higher, 65°C or higher, 70°C or higher, 75°C or higher, 80°C or higher, 85°C or higher, 90°C or higher, 95°C or higher, 100°C or higher, 150°C or lower, 145°C or lower, 140°C or lower, 135°C or lower, 130°C or lower, 125°C or lower, 120°C or lower, 115°C or lower, 110°C or lower, or 105°C or lower.

[0079] In one embodiment, the extraction step may be performed for a time of 5 minutes or more, 10 minutes or more, 15 minutes or more, 20 minutes or more, 25 minutes or more, 30 minutes or more, 60 minutes or less, 55 minutes or less, 50 minutes or less, 45 minutes or less, 40 minutes or less, or 35 minutes or less.

[0080] In one embodiment, after the extraction step, a step of purifying the extracted material may be further included.

[0081] In one embodiment, when extracting each single powder solution, after the extraction step, a step of mixing each extracted extract may be further included.

[0082] In one embodiment, the method for preparing the composition may further include the step of roasting one or more of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves.

[0083] In one embodiment, the roasting treatment may include stirring continuously for 10 to 60 minutes at 100 to 300°C.

[0084] In one embodiment, the roasting treatment may be performed at 100°C or higher, 110°C or higher, 120°C or higher, 130°C or higher, 140°C or higher, 150°C or higher, 160°C or higher, 170°C or higher, 180°C or higher, 190°C or higher, 200°C or higher, 300°C or lower, 290°C or lower, 280°C or lower, 270°C or lower, 260°C or lower, 250°C or lower, 240°C or lower, 230°C or lower, 220°C or lower, or 210°C or lower.

[0085] In one embodiment, the roasting treatment may involve stirring continuously for a time of 10 minutes or more, 15 minutes or more, 20 minutes or more, 25 minutes or more, 30 minutes or more, 35 minutes or more, 60 minutes or less, 55 minutes or less, 50 minutes or less, 45 minutes or less, 40 minutes or less, or 35 minutes or less.

[0086] One embodiment of the present disclosure is a cosmetic composition comprising the above-mentioned antioxidant or skin moisturizing composition.

[0087] In one embodiment, the cosmetic composition may further include one or more of an antioxidant, a preservative, an absorbent, an exfoliant, a thickener, a sunscreen formulation, an emulsifier, a surfactant, a fragrance, a deodorizer, a stabilizer, a skin protectant, and a pH adjuster.

[0088] In one embodiment, the cosmetic composition may be any one selected from the group consisting of a skincare cosmetic composition, a makeup cosmetic composition, a haircare cosmetic composition, and a body care cosmetic composition, but is not limited thereto.

[0089] In one embodiment, the skincare cosmetic composition may be a softening lotion, toner, ampoule, essence, cream, nourishing cream, massage cream, pack, gel, lotion, powder, spray, or concealer stick, but is not limited thereto. These compositions may be prepared according to conventional methods in the field.

[0090] In one embodiment, the makeup cosmetic composition may be a makeup base, liquid foundation, stick foundation, BB cream, CC cream, concealer, lipstick, lip gloss, primer, sun lotion, sun cream, and sun gel, but is not limited thereto.

[0091] In one embodiment, the hair care cosmetic composition may be a shampoo, rinse, treatment, essence, ampoule, wax, mousse, gel, spray, glaze, and hair lotion, but is not limited thereto.

[0092] In one embodiment, the body care cosmetic composition may be a body lotion, body cream, body oil, and body scrub, but is not limited thereto.

[0093] One embodiment of the present disclosure is a sanitary product comprising the above-mentioned antioxidant or skin moisturizing composition.

[0094] In one embodiment, the sanitary product may be any one selected from the group consisting of wet wipes, tissues, diapers, sanitary pads, sanitary wipes, moist wipes, cleaning wipes, facial wipes, skincare wipes, and antiperspirant wipes, but is not limited thereto.

[0095] Hereinafter, embodiments of the present disclosure will be described in detail. However, the following embodiments are provided merely to facilitate a better understanding of the present disclosure and do not limit the scope of the present disclosure.

[0096] Examples

[0097] Preparation of a single extract

[0098] 10 g each of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves were finely ground, and 200 g of distilled water was added to prepare four powder solutions. Subsequently, the powder solutions of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves were extracted by shaking at 90°C for 30 minutes, then naturally cooled to 30°C in a sealed state, and the remaining camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves were removed by filtering through filter paper. The filtrate was first filtered through a membrane filter with a pore size of 1.0 μm, and then secondarily filtered through a membrane filter with a pore size of 0.45 μm to prepare extracts of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves as listed in Table 1 below.

[0099] Comparative Example 1 5% camphor leaf single extract Comparative Example 2 5% bay leaf single extract Comparative Example 3 5% bitter melon fruit single extract Comparative Example 4 5% mugwort leaf single extract

[0100] Preparation of single roasted extract

[0101] 10 g of dried camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves were placed in a container with a diameter of 28 cm and a height of 10 cm, and dry heat roasting treatment was performed for 30 minutes while continuously stirring each while maintaining the internal temperature of the container at 160±5℃. Afterward, the roasted raw materials were finely ground.

[0102] Subsequently, 200 g of distilled water was mixed with each of the above-mentioned ground materials and extracted by shaking at 90°C for 30 minutes. Afterward, the mixture was cooled naturally to 30°C in a sealed state, and the remaining extracts of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves were removed by filtering through filter paper. The filtrate was first filtered through a membrane filter with a pore size of 1.0 μm, and then secondarily filtered through a membrane filter with a pore size of 0.45 μm to prepare roasted extracts of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves as described in Table 2 below.

[0103] Comparative Example 5 5% roasted camphor leaf single extract Comparative Example 6 5% roasted bay leaf single extract Comparative Example 7 5% roasted bitter melon fruit single extract Comparative Example 8 5% roasted mugwort leaf single extract

[0104] Preparation of complex extracts

[0105] According to the extraction preparation methods of Comparative Examples 1 to 8 above, extracts were prepared by including each natural ingredient in a weight ratio based on the dry weight of each natural ingredient as listed in Table 3 below. At this time, as shown in Table 4, the total content of the complex extract was fixed at 5% based on the total weight of the composition.

[0106] camphor tree leaves bay leaves bitter melon mugwort leaves Roasting Example 1 1 1 1 1 x Example 2 2 1 1 2 x Example 3 1 1 2 2 x Example 4 1 2 2 1 x Example 5 2 2 1 1 x Example 6 2 1 2 1 x Example 7 1 2 1 2 x Example 8 1 1 3 3 x Example 9 3 1 1 3 x Example 10 3 1 3 1 x Example 11 3 3 1 1 x Example 12 1 3 3 1 x Example 13 1 3 1 3 x Example 14 1 1 1 1 o Example 15 2 1 1 2 o Example 16 1 1 2 2 o Example 17 1 2 2 1 o Example 18 2 2 1 1 o Example 19 2 1 2 1 o Example 20 1 2 1 2 o Example 21 1 1 3 3 o Example 22 3 1 1 3 o Example 23 3 1 3 1 o Example 24 3 3 1 1 o Example 25 1 3 3 1 o Example 26 1 3 1 3 o

[0107] Extract content (weight%) camphor tree leaves bay leaves bitter melon mugwort leaves Comparative Example 1 5 - - - Comparative Example 2 - 5 - - Comparative Example 3 - - 5 - Comparative Example 4 - - - 5 Examples 1-26 5

[0108] Confirmation of the effect of roasting treatment on a single extract

[0109] (1) Total polyphenol compound content

[0110] Natural polyphenolic compounds are one of the secondary metabolites widely distributed in the plant kingdom, and are known to have various structures and molecular weights, easily bind to proteins and other macromolecules, and possess various physiological activities such as antioxidant and anti-inflammatory effects.

[0111] Total phenolic compounds (TPC) were analyzed by modifying the colorimetric method using Folin-Ciocalteuphenol reagent. 10 μL each of the single extract and the roasted extract were mixed with 10 μL of 1 N Folin-Ciocalteuphenol, reacted at room temperature for 5 minutes, and then 130 μL of purified water and 20 μL of 20% (v / v) Na2CO3 were added to the mixture. After standing in a dark room for 1 hour, the absorbance was measured at 750 nm using a UV-Vis spectrophotometer. Gallic acid was used as a standard, and the total polyphenol content (mg / g) was calculated from the standard curve.

[0112] As shown in Figure 1, the total polyphenol compound content increased after roasting treatment compared to before roasting treatment. The highest polyphenol content was observed in camphor leaf and mugwort extracts, and the polyphenol compounds in bitter melon fruit extract increased by about 60.5% through roasting treatment.

[0113] (2) Total flavonoid content

[0114] Flavonoid compounds have a structure consisting of three rings combined with two aromatic rings, and possess various skin-improving activities due to the functional groups within each ring. Flavonoid compounds are found in large quantities in various plants and act as antioxidants and anti-inflammatory agents.

[0115] Total flavonoid content (TFC) was determined by mixing 20 μL each of the single extract and the roasted extract with 4 μL of a 10% aluminum chloride solution. Subsequently, 4 μL of a 1 M potassium acetate solution and 172 μL of ethyl alcohol were added. After standing at room temperature for 40 minutes, the absorbance was measured at 415 nm. A standard curve was prepared using quercetin, and the total flavonoid content (mg / g) was calculated from the standard curve.

[0116] As shown in Figure 2, the total flavonoid content of each natural product increased after roasting compared to before roasting, and the total flavonoid content in bay leaf extract increased by approximately 33.3%. In addition, mugwort extract showed the highest flavonoid content.

[0117] Confirmation of the antioxidant effect of the complex extract

[0118] (1) DPPH radical scavenging activity measurement

[0119] DPPH is a relatively stable free radical that is reduced by aromatic amines, etc., and can be used to identify plant antioxidants through a decolorization reaction of the radical aqueous solution into a deep purple color.

[0120] DPPH radical scavenging activity was determined by adding 100 μL of 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution to 100 μL of the above-mentioned untreated and roasted complex extracts, mixing, and letting it stand for 30 minutes, after which the absorbance was measured at 515 nm using a spectrophotometer. The control group was measured in the same manner by adding a solvent instead of the sample, and to correct for the absorbance value due to the color of the sample itself, methanol was added instead of 0.2 mM DPPH and the absorbance was measured in the same manner. Ascorbic acid was used as a positive control, and the absorbance was calculated as follows to compare with that of the sample.

[0121]

[0122] As shown in Fig. 3, higher DPPH radical scavenging activity was confirmed in complex extracts (Examples 1 to 13) compared to single extracts (Comparative Examples 1 to 4), and similar results were observed in the roasted extracts, and higher DPPH radical scavenging activity was confirmed in complex extracts (Examples 14 to 26) compared to single extracts (Comparative Examples 5 to 8). While the average value of the DPPH radical scavenging activity of each of the above single extracts was 69.1%, in the case of the complex extract containing camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, the DPPH radical scavenging activity was found to be 76.4 to 93.1% based on the total weight of the composition, even though the total amount of natural materials contained was the same as that of the single extracts. This was significantly higher than the expected average value, confirming that there is a synergistic effect in the DPPH antioxidant activity.

[0123] In addition, higher DPPH radical scavenging activity was confirmed in the roasted complex extracts (Examples 14 to 26) compared to the untreated complex extracts (Examples 1 to 13), and a radical scavenging activity increased by up to 21% compared to the untreated complex extracts.

[0124] In addition, as in Examples 2, 9, 15 and 22, when the content of camphor leaves and mugwort leaves in the complex extract was higher than that of bay leaves and bitter melon fruit based on the total weight of the composition, it exhibited superior DPPH radical scavenging ability.

[0125] (2) ABTS radical scavenging ability measurement

[0126] ABTS can measure radical scavenging activity against both hydrophilic and hydrophobic components. For the measurement of ABTS (2,2-azino-bis-3-ethylbenzo-thiazolin-6-sulfonate) radical scavenging activity, an ABTS radical solution was prepared by mixing 7 mM ABTS reagent with 2.4 mM potassium persulfate in a 1:1 ratio and reacting at room temperature for 16 hours. The concentration of the radical solution was adjusted using purified water to an absorbance of 0.650 ± 0.020 at 734 nm. 100 μL of the ABTS radical solution was reacted with 100 μL of the above-mentioned untreated and roasted complex extracts for 10 minutes, and the decrease in absorbance at 734 nm was measured. Ascorbic acid was used as a positive control, and the results were calculated as follows to compare with the absorbance of the samples.

[0127]

[0128] As shown in Fig. 4, higher ABTS radical scavenging activity was confirmed in the complex extracts (Examples 1 to 13) compared to the single extracts (Comparative Examples 1 to 4), and similar results were observed in the roasted extracts, and higher ABTS radical scavenging activity was confirmed in the complex extracts (Examples 14 to 26) compared to the single extracts (Comparative Examples 5 to 8). While the average value of the ABTS radical scavenging activity of each of the above single extracts was 47.4%, in the case of the complex extract containing camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, the ABTS radical scavenging activity was found to be 55.7 to 77.1% based on the total weight of the composition, even though the total amount of natural materials contained was the same as that of the single extracts. This was significantly higher than the expected average value, confirming that there is a synergistic effect in the ABTS antioxidant activity (Fig. 4).

[0129] In addition, higher ABTS radical scavenging activity was confirmed in the roasted complex extracts (Examples 14 to 26) compared to the untreated complex extracts (Examples 1 to 13), and a radical scavenging activity increased by up to 11.7% compared to the untreated complex extracts. Furthermore, as in Examples 2, 9, 15 and 22, superior scavenging activity was exhibited when the content of camphor leaves and mugwort leaves in the complex extract was higher than that of bay leaves and bitter melon fruit based on the total weight of the composition.

[0130] Confirmation of the moisturizing effect of the complex extract

[0131] Hyaluronidase (HAase) is an enzyme that cleaves the glucosamine bonds of hyaluronic acid (HA), a glycosaminoglycan polymer. Hyaluronic acid inhibits phagocytosis, a factor in inflammation, and acts as a buffer by supplying moisture to the skin because it exists with a large amount of water. Additionally, the activity of hyaluronidase is inhibited by anti-allergic or anti-inflammatory substances, and anti-inflammatory and moisturizing effects can be expected by inhibiting hyaluronidase activity.

[0132] Hyaluronidase inhibitory activity was measured by modifying N-acetylglucosamine formed from sodium hyaluronic acid (HA) into a glucooxazoline derivative, developing the color with p-dimethylaminobenzaldehyde (DMAB), and measuring the absorbance to determine enzyme activity. 50 μL of hyaluronidase (7,900 U / mL) dissolved in 0.1 M acetate buffer (pH 3.5) was mixed with 100 μL of the above-mentioned untreated and roasted complex extract and incubated at 37°C for 20 minutes, then 12 mg / mL of sodium hyaluronic acid dissolved in 0.1 mL of 12.5 mM CaCl2 acetate buffer (pH 3.5) was added and incubated again for 40 minutes. Subsequently, 100 μL of 0.4 N potassium tetraborate and 100 μL of 0.4 N NaOH solution were added to the reaction mixture, heated in a water bath for 3 minutes, and then completely cooled. 1000 μL of DMAB reagent was added as a chromogenic agent to the cooled reaction mixture and incubated at 37°C for 20 minutes. The absorbance was then measured at 585 nm, and the inhibitory activity was calculated according to the following formula.

[0133]

[0134] As shown in Fig. 5, the hyaluronidase inhibitory activity was higher in the complex extracts (Examples 1 to 13) compared to the single extracts (Comparative Examples 1 to 4), and similar results were observed in the roasted extracts, with the complex extracts (Examples 14 to 26) showing higher hyaluronidase inhibitory activity compared to the single extracts (Comparative Examples 5 to 8). While the average value of the hyaluronidase removal rate of each of the above single extracts was 5.3%, the hyaluronidase removal rate of the complex extract containing camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves was 9.9 to 17.0%, even though the total amount of natural materials contained based on the total weight of the composition was the same as that of the single extracts. This was significantly higher than the expected average value, confirming that there was a synergistic effect on hyaluronidase inhibitory activity (Fig. 5).

[0135] In addition, the roasted complex extracts (Examples 14 to 26) showed higher hyaluronidase inhibitory activity compared to the untreated complex extracts (Examples 1 to 13). Furthermore, based on the total weight of the composition, the complex extracts in which the content of camphor leaves and mugwort leaves was higher than that of bay leaves and bitter melon fruit (Examples 2, 9, 15, and 22) showed higher inhibitory activity.

[0136] The following describes an example of a formulation of a composition containing a complex extract according to one embodiment of the present specification, but it can be applied to various other formulations, and this is intended only to provide a specific description and not to limit the present specification.

[0137] [Formulation Example 1] Softening lotion (skin lotion)

[0138] Softening lotion was prepared using a conventional method according to the composition listed in the table below.

[0139]

[0140] [Formulation Example 2] Nourishing Cream

[0141] A nourishing cream was prepared using a conventional method according to the composition listed in the table below.

[0142]

[0143] [Formulation Example 3] Massage cream

[0144] A massage cream was prepared using a conventional method according to the composition listed in the table below.

[0145]

[0146] [Formulation Example 4] Pack

[0147] A pack was manufactured using a conventional method according to the composition listed in the table below.

[0148]

[0149] [Formulation Example 5] Gel

[0150] A gel was prepared using a conventional method according to the composition listed in the table below.

[0151]

Claims

Claim 1 An antioxidant or skin moisturizing composition comprising a complex extract of camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves, wherein the complex extract comprises camphor tree leaves : bay leaves : bitter melon fruit : mugwort leaves in a weight ratio of 1-3 : 1-3 : 1-3 : 1-3 based on the dry weight of each natural product used in the extraction. Claim 2 A composition according to claim 1, wherein the weight ratio is 2-3 : 1 : 1 : 2-3. Claim 3 A composition according to claim 1, wherein the content of the complex extract is 0.0001 to 90% based on the total weight of the composition. Claim 4 A composition according to claim 3, wherein the content of the complex extract is 0.001 to 10% based on the total weight of the composition. Claim 5 A composition according to claim 1, wherein one or more of the above-mentioned camphor tree leaves, bay leaves, bitter melon fruit, and mugwort leaves are roasted before being extracted with an extraction solvent. Claim 6 A composition according to claim 1, wherein the complex extract is extracted with one or more extraction solvents selected from water, purified water and distilled water. Claim 7 A method for preparing a composition according to any one of claims 1 to 6, comprising the steps of: grinding camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves; adding an extraction solvent to the ground powder; and extracting by heating the mixture of the powder and the extraction solvent at 50 to 150°C for 5 to 60 minutes. Claim 8 A method according to claim 7, further comprising the step of roasting one or more of camphor leaves, bay leaves, bitter melon fruit, and mugwort leaves before extracting them with an extraction solvent. Claim 9 A method according to claim 8, wherein the roasting treatment comprises stirring continuously at 100 to 300°C for 10 to 60 minutes. Claim 10 In claim 7, the extraction solvent is one or more selected from water, purified water and distilled water. Claim 11 A cosmetic composition comprising the composition of any one of claims 1 to 6. Claim 12 A cosmetic composition according to claim 11, further comprising one or more of an antioxidant, a preservative, an absorbent, an exfoliating agent, a thickener, a sunscreen formulation, an emulsifier, a surfactant, a fragrance, a deodorizer, a stabilizer, a skin protectant, and a pH adjuster. Claim 13 In claim 11, the cosmetic composition is any one selected from the group consisting of a skincare cosmetic composition, a makeup cosmetic composition, a hair care cosmetic composition, and a body care cosmetic composition. Claim 14 A sanitary product comprising the composition of any one of paragraphs 1 to 6. Claim 15 In claim 14, the above sanitary product is any one selected from the group consisting of wet wipes, tissues, diapers, sanitary pads, sanitary wipes, moist wipes, cleaning wipes, facial wipes, skincare wipes, and antiperspirant wipes. Claim 16 delete Claim 17 delete

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