Liposome composition for enhancing skin absorption and efficacy through adhesion enhancement and manufacturing method thereof

Abstract

The present invention relates to liposomes and cosmetic compositions, etc. A liposome composition according to one aspect is safe without irritating the human body, and when loaded with skin active substances such as collagen, its skin adhesion and absorption capacity are increased compared to an aqueous solution, thereby significantly enhancing the efficacy of the skin active substances; thus, by applying such a liposome composition to a cosmetic composition, the prevention and improvement of skin aging can be promoted.

Description

Technology Field

[0001] The present invention relates to liposomes and cosmetic compositions, etc. Background Technology

[0003] The skin, the body's outermost membrane composed of the epidermis, dermis, and subcutaneous fat layer, is vulnerable to oxidative stress due to direct exposure to various harmful environmental factors such as ultraviolet rays, near-infrared rays, and pollution. This oxidative stress causes aging phenomena such as wrinkles and pigmentation; among these, reactive oxygen species (ROS) induced by photoaging—considered the leading cause of skin aging—accelerate this process.

[0004] Because ROS are highly reactive, they oxidize lipids, proteins, and DNA, which are components of skin cells. In particular, ROS promote the expression of matrix metalloproteinases (MMPs), which are collagen-hydrolyzing enzymes, thereby reducing collagen fibers in the skin and consequently increasing wrinkles. Furthermore, since the production of collagen in the skin decreases rapidly with age, additional collagen supplementation is essential to maintain elastic and healthy skin.

[0005] Collagen is a protein that makes up more than 70% of the dermis layer and is an essential component for maintaining skin elasticity. Although various types of collagen-related ingredients are being developed for the prevention of skin aging and wrinkle improvement, collagen has a relatively large molecular weight and is water-soluble, resulting in low affinity for intercellular lipids in the stratum corneum; therefore, it is difficult to expect high skin absorption effects with conventional emulsification or solubilization formulations.

[0006] Meanwhile, liposomes are representative biocompatible skin delivery systems characterized by their ability to carry both hydrophilic and hydrophobic substances, with water-soluble components solubilized in the aqueous core and lipid-soluble components solubilized in the phospholipid membrane. Accordingly, the inventors developed liposomes composed of optimal amounts to enhance the skin absorption of skin-active substances, including collagen, which possess skin regeneration, anti-aging, wrinkle improvement, elasticity enhancement, and skin whitening activities. Furthermore, by evaluating the skin adhesion and permeability of the collagen-carrying liposome composition, as well as changes in protein expression levels within the skin, the inventors confirmed the potential for improved efficacy resulting from increased absorption of the skin-active substances. The problem to be solved

[0008] One aspect provides a liposome composition with improved skin adhesion and absorption capacity.

[0009] Another aspect provides a cosmetic composition for improving skin wrinkles or enhancing elasticity, comprising the above-mentioned liposome composition as an active ingredient.

[0010] Another aspect provides a topical skin composition for improving skin wrinkles or enhancing elasticity, comprising the above-mentioned liposome composition as an active ingredient. means of solving the problem

[0012] One aspect provides a liposome composition with improved skin adhesion and absorption capacity, comprising an oil phase comprising phospholipids, cholesterol, ceramides, and polyols; and an aqueous phase. Specifically, at least one of the oil phase and the aqueous phase may contain a skin active substance, and more specifically, the aqueous phase may contain a skin active substance. The liposome composition may further include additives such as preservatives, pH adjusters, surfactants, antioxidants, UV blockers, pigments, dyes, fragrances, stabilizers, and thickeners, as well as purified water, which may be appropriately selected by those skilled in the art. For example, the aqueous phase may further include purified water and preservatives in addition to the skin active substance.

[0013] The term 'liposome' in this specification refers to a spherical or elliptical structure surrounded by a fine lipid membrane, having the form of a lipid bilayer or multilayer vesicle. The above lipid is an amphiphilic lipid and specifically may be a phospholipid, and the above phospholipid may be any one selected from the group consisting of lecithin, phosphatidylcholine, phosphatidic acid, phosphatidylserine, phosphatidyl glycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol, cardiolipin, plasmalogen, dicetylphosphate, distearoylphosphatidylcholine, dioleoylphosphatidylethanolamine, dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, dipalmitoylphosphatidylserine, eleostearoylphosphatidylcholine, eleostearoylphosphatidylethanolamine, eleostearoylphosphatidylserine, and any combination thereof, and more specifically may be lecithin.

[0014] The above 'lecithin' can be naturally produced as a mixture of diglycerides of stearic acid, palmitic acid, and oleic acid, and the composition of fatty acids may vary depending on the source. In one embodiment of the present invention, hydrogenated lecithin produced by controlling the hydrogenation (hydrogenation addition) of lecithin was used.

[0015] The above phospholipid may be included in a range of 0.1 to 5 parts by weight, 0.3 to 5 parts by weight, 0.5 to 5 parts by weight, 0.1 to 3 parts by weight, 0.3 to 3 parts by weight, 0.5 to 3 parts by weight, 0.1 to 1 part by weight, 0.3 to 1 part by weight, 0.5 to 1 part by weight, 0.1 to 0.7 parts by weight, 0.3 to 0.7 parts by weight, and 0.5 to 0.7 parts by weight relative to 100 parts by weight of the liposome composition.

[0016] In this specification, the term 'cholesterol' refers to a type of lipid, specifically an organic substance of the steroid family with hydrophobic properties, which serves to provide stability to the liposome structure. In this regard, in one embodiment of the present invention, it was confirmed that in the case of liposomes not containing cholesterol, separation occurs immediately after preparation.

[0017] The above cholesterol may be included in the range of 0.1 to 3 parts by weight, 0.25 to 3 parts by weight, 0.1 to 1 part by weight, 0.25 to 1 part by weight, 0.1 to 0.7 parts by weight, 0.25 to 0.7 parts by weight, 0.1 to 0.5 parts by weight, 0.25 to 0.5 parts by weight, and 0.1 to 0.25 parts by weight, based on 100 parts by weight of the liposome composition.

[0018] The term 'ceramide' in this specification refers to a molecule composed of sphingosine and fatty acids as a type of sphingolipid, which promotes the differentiation of keratinocytes to form a protective barrier in the stratum corneum of the skin and maintain skin moisture.

[0019] The above ceramide may be included in an amount of 0.005 to 0.1 parts by weight, 0.005 to 0.05 parts by weight, 0.005 to 0.03 parts by weight, 0.01 to 0.1 parts by weight, 0.01 to 0.05 parts by weight, or 0.01 to 0.03 parts by weight per 100 parts by weight of the liposome composition.

[0020] The term 'polyol' in this specification refers to an aliphatic compound having two or more hydroxyl groups (-OH). The polyol may be any one selected from the group consisting of propylene glycol, dipropylene glycol, butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, and any combination thereof, but is not particularly limited thereto. In one embodiment of the present invention, dipropylene glycol was used.

[0021] The above polyol may be included in an amount of 1 to 20 parts by weight, 1 to 15 parts by weight, 1 to 10 parts by weight, 3 to 20 parts by weight, 3 to 15 parts by weight, 3 to 10 parts by weight, 5 to 20 parts by weight, 5 to 15 parts by weight, 5 to 10 parts by weight, 7 to 20 parts by weight, 7 to 15 parts by weight, 7 to 10 parts by weight, 10 to 20 parts by weight, and 10 to 15 parts by weight, based on 100 parts by weight of the liposome composition.

[0022] The above cholesterol, ceramide, and polyol together with phospholipid (lecithin) form the lipid portion of the liposome composition. In one embodiment of the present invention, hydrogenated lecithin, cholesterol, ceramide, and dipropylene glycol were heated and dissolved at 70 to 80°C according to their respective contents, then added to and stirred in an aqueous phase at room temperature containing collagen, and subsequently, the mixture was subjected to high-pressure emulsification to finally produce a liposome.

[0023] In this specification, the term "skin active substance" may refer to any substance capable of imparting useful activity to an individual, such as skin regeneration, anti-aging, wrinkle improvement and elasticity enhancement, skin whitening activity, etc. Specifically, the skin active substance may be one or more compounds selected from the group consisting of collagen, hyaluronic acid, niacinamide, adenosine, vitamin C (ascorbic acid), retinol, tocopherol, coenzyme Q10, and any combination thereof, or derivatives thereof, but is not particularly limited thereto. Depending on the type of skin active substance supported within the liposome of the present invention, the specific efficacy of a cosmetic composition or topical skin preparation to which the same is applied may vary, and the type and content of the skin active substance supported may be appropriately selected by a person skilled in the art.

[0024] The term 'collagen' in this specification refers to a fibrous protein that makes up most of all connective tissues in the body, such as the skin and cartilage of mammals, and specifically refers to 'hydrolyzed collagen (peptide)' which is produced by treating collagen with a proteolytic enzyme to reduce its molecular weight, and the molecular weight of the hydrolyzed collagen may be about 500 to 4000 Da.

[0025] The above skin active substance may be included in the range of 0.1 to 10 parts by weight, 0.3 to 10 parts by weight, 0.5 to 10 parts by weight, 1 to 10 parts by weight, 3 to 10 parts by weight, 5 to 10 parts by weight, 0.1 to 5 parts by weight, 0.3 to 5 parts by weight, 0.5 to 5 parts by weight, 1 to 5 parts by weight, 0.1 to 3 parts by weight, 0.3 to 3 parts by weight, 0.5 to 3 parts by weight, 1 to 3 parts by weight, 0.1 to 1 part by weight, 0.3 to 1 part by weight, 0.5 to 1 part by weight, 0.1 to 0.7 parts by weight, 0.3 to 0.7 parts by weight, and 0.5 to 0.7 parts by weight, based on 100 parts by weight of the liposome composition of the present invention.

[0026] However, if an excess amount of collagen exceeding 5 parts by weight per 100 parts by weight of the liposome composition is included as a skin active substance, a haze phenomenon appears in which the liposome composition appears cloudy, making it difficult to use in solubilized and emulsified formulations. Therefore, the collagen may be included in the range of 0.1 to 5 parts by weight, 0.3 to 5 parts by weight, 0.5 to 5 parts by weight, 1 to 5 parts by weight, 0.1 to 3 parts by weight, 0.3 to 3 parts by weight, 0.5 to 3 parts by weight, 1 to 3 parts by weight, 0.1 to 1 part by weight, 0.3 to 1 part by weight, 0.5 to 1 part by weight, 0.1 to 0.7 parts by weight, 0.3 to 0.7 parts by weight, and 0.5 to 0.7 parts by weight per 100 parts by weight of the liposome composition.

[0027] In this specification, the term "skin adhesion" refers to the degree of residual collagen remaining on the skin after the liposome composition is applied to the skin and washed off. It can be evaluated that the better the skin adhesion of the collagen contained in the liposome composition, the better the degree of absorption into the skin. In one embodiment of the present invention, the concentration of collagen remaining on an artificial membrane (Strat-M, Merck) was calculated through quantification using a BCA assay based on the number of times each liposome composition was applied and washed with purified water, and was also visually confirmed using IVIS.

[0028] The term 'skin absorption capacity' in this specification may be used interchangeably with 'skin permeability,' which refers to the degree to which skin active substances, such as collagen, are absorbed into the skin. In one embodiment of the present invention, a collagen-containing liposome composition combined with the fluorescent reagent FITC was applied to a 3D skin having only an epidermal layer, and the amount of collagen permeability was confirmed using a confocal scanning microscope.

[0029] In addition, as the skin permeability of collagen was improved, the actual efficacy exerted within the skin was evaluated. It was confirmed that all genes related to collagen synthesis—type 1 collagen, keratin 5, keratin 14, and involucrin related to keratinocyte differentiation—and genes related to the stratum corneum dendrite—aquaporin—were expressed more highly when treated with a liposome composition loaded with collagen compared to an aqueous solution (Fig. 6). In other words, it was confirmed that the liposome composition of the present invention improves the skin adhesion and absorption capacity of collagen, and thereby has the effect of actually promoting collagen synthesis or keratinocyte differentiation within the skin.

[0031] Another aspect provides a cosmetic composition for improving skin wrinkles or enhancing elasticity, comprising the liposome composition as an active ingredient. Specifically, the other aspect may be a cosmetic composition for skin regeneration, anti-aging, wrinkle improvement, elasticity enhancement, or skin whitening, and the same as described above applies equally to the cosmetic composition.

[0032] In this specification, the term "skin regeneration" refers to a series of reactions in which skin tissue is reorganized in response to skin aging or damage caused to the skin, and specifically, this may be accomplished through the differentiation, proliferation, and migration of keratinocytes. In this specification, the term "anti-aging" refers to any action that prevents functional degeneration due to the aging of skin cells and tissues, and "skin whitening" refers to making the skin white; specifically, it may refer to alleviating or improving various pigmentations, including melasma and freckles, caused by excessive synthesis or deposition of melanin.

[0033] In this specification, the term 'skin wrinkle improvement' refers to any action that reduces or slows down the rate of wrinkle formation caused by a decrease in collagen fibers or a decrease in the amount of collagen produced within the skin, and 'elasticity enhancement' may refer to any action that increases the elasticity and restorability of the skin due to an increase in the absorption or production of collagen within the skin.

[0034] The above cosmetic composition may be manufactured in various forms, for example, the composition may be manufactured in any one formulation selected from the group consisting of lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, suspension, gel, powder, paste, mask pack or sheet, and aerosol, and the composition of such formulation may be manufactured according to methods conventional in the field. The above cosmetic composition may further include preservatives, stabilizers, surfactants, solvents, moisturizers, emollients, UV absorbers, antiseptics, disinfectants, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, cooling agents or limiting agents, etc. The amount of additional ingredients such as the above moisturizer can be easily selected by a person skilled in the art within a range that does not impair the purpose and effect of the present invention, and the amount may be 0.001 to 5 weight%, specifically 0.01 to 3 weight%, based on the total weight of the composition.

[0036] Another aspect is to provide a topical skin composition for improving skin wrinkles or enhancing elasticity, comprising the above-described liposome composition as an active ingredient. Specifically, another aspect may be a topical skin composition for skin regeneration, anti-aging, wrinkle improvement, elasticity enhancement, or skin whitening, and the same parts as described above apply equally to the topical skin composition.

[0037] The above-mentioned topical preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal patch, drug-containing bandage, lotion, or a combination thereof. The above-mentioned topical preparation may be appropriately formulated as needed with ingredients commonly used in topical preparations for the skin, such as cosmetics or pharmaceuticals, for example, aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, UV absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or combinations thereof. In addition, the above external skin preparation may be appropriately formulated by a person skilled in the art with metal chelating agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid; caffeine, tannin, bellapamil, licorice extract, glablidin, hot water extract of the fruit of Carin, various herbal medicines, pharmaceuticals such as tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts thereof; and sugars such as vitamin C, magnesium ascorbate phosphate, ascorbate glucoside, arbutin, kojic acid, glucose, fructose, and trehalose. Effects of the invention

[0039] A liposome composition according to one aspect is safe without irritating the human body, and when loaded with skin active substances such as collagen, its skin adhesion and absorption capacity are increased compared to an aqueous solution, thereby significantly enhancing the efficacy of the skin active substances; thus, by applying such a liposome composition to a cosmetic composition, the prevention and improvement of skin aging can be promoted. Brief explanation of the drawing

[0041] Figure 1 shows the results of measuring particle size and zeta potential for 21 days for a liposome composition of one aspect. Figure 2 shows an image confirming the structure of a liposome particle of one aspect through Cryo-TEM. Figure 3 is a graph showing the results of a skin adhesion test using an artificial membrane (Strat-M, Merck) and the amount of collagen remaining on the artificial membrane according to the number of washes. Figure 4 shows the results of a skin adhesion test using an artificial membrane (Strat-M, Merck), and the amount of collagen remaining on the artificial membrane according to the number of washes was confirmed using IVIS measurement images. Figure 5 shows the results of confirming skin penetration ability using a confocal scanning microscope for a liposome composition and an aqueous solution of one aspect. Figure 6 shows the qRT-PCR results for various skin-related genes after applying a liposome composition and aqueous solution of one aspect. Specific details for implementing the invention

[0042] The following examples will be explained in more detail. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

[0044] Examples and Comparative Examples: Preparation of Liposomes

[0045] Aqueous solutions and liposomes of the Examples and Comparative Examples having the compositions (unit: weight%) shown in Table 1 below were prepared. Specifically, excluding Comparative Examples 1 and 2, the remaining components were weighed according to their respective contents and contents. Phase A (oil phase) was heated and dissolved to 70–80°C, then added to Phase B (aqueous phase) at room temperature containing collagen, and stirred with an Azimixer for 5 minutes. After the first stirring, high-pressure emulsification was performed under conditions of 1,000 bar and 3 cycles to finally prepare liposomes. Comparative Examples 1 and 2 are aqueous solutions in which collagen is dissolved in purified water.

[0046] ingredient Content (weight%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Example 1 Example 2 A Hydrogenated lecithin - - 0.50 0.50 0.50 0.50 0.50 cholesterol - - - 0.25 0.25 0.25 0.25 Ceramide - - 0.01 0.01 0.01 0.01 0.01 Dipropylene glycol - - 10.00 10.00 10.00 10.00 10.00 B purified water Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount hydrolyzed collagen 0.50 1.00 1.00 5.00 10.00 0.50 1.00 antiseptic Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount

[0048] Experimental Example 1. Evaluation of Physical Properties and Confirmation of Stability of Liposome Particles

[0049] A dynamic light scattering device (SZ-100, HORIBA) was used to measure the particle sizes of the above Examples and Comparative Examples 3 to 5. Table 2 below shows the average particle size immediately after preparation, and the average particle size increased as the collagen content increased. In the case of Comparative Example 3, separation occurred immediately after preparation, indicating that cholesterol has a significant effect on the stability of the liposomes. A hazy phenomenon was observed in Comparative Examples 4 and 5, which had a collagen content of 5% or more. In contrast, Examples 1 and 2 showed a particle size distribution of 200 nm or less, and their appearance was transparent or translucent, confirming that they can be utilized in various solubilization and emulsion formulations. Meanwhile, as a result of measuring the particle size and zeta potential of Example 2 for 21 days, it was confirmed that it is a stable liposome with almost no change in both particle size and zeta potential (Fig. 1).

[0050] Example 1 Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 hydrolyzed collagen Content (%) 0.50 1.00 1.00 5.00 10.00 Average particle size (nm) 134.0 151.90 separation 307.4 559.2 transparency transparency translucent separation Turbidity Turbidity

[0052] In addition, cryo-TEM (Cryogenic transmission electron microscopy, Tecnai F20, FEI) was used to analyze the structure of liposome particles. First, 5 μL of liposomes were loaded onto a 200 mesh carbon lacey film Cu-grid, and then plunge-frozen by immersing them in liquefied ethane (approx. -170°C) using an in vitrobot. The resulting frozen samples were observed under an acceleration voltage of 200 kV. As a result, it was confirmed that liposomes formed bilayer and multilayer structures, as shown in Figure 2.

[0054] Experimental Example 2. Evaluation of Skin Adhesion

[0055] The degree of skin adhesion was evaluated using Example 1 and Comparative Example 1, which had the same collagen content of 0.5%. Specifically, the fluorescent reagent FITC (Fluorescein-5-isothiocyanate) was bound to collagen, and this was applied to Example 1 and Comparative Example 1 to prepare an aqueous collagen solution and a collagen liposome, respectively. To evaluate skin adhesion, each sample was applied onto an artificial membrane (Strat-M, Merck) and dried for 1 hour. Subsequently, the concentration of the washed-out collagen was calculated based on the number of washes with 1 mL of purified water to determine the residual amount remaining on the artificial membrane. At this time, the number of washes was fixed at 4 times, at which the adhesion rate showed no further tendency to decrease. In addition, the concentration of the remaining collagen was calculated through quantification using a BCA assay and visually confirmed using an IVIS (In vivo optical imaging system).

[0056] When washed up to 4 times with purified water, the amount of collagen remaining in the artificial membrane, i.e., the skin, was 49% for Comparative Example 1 and 76% for Example 1, which was higher when contained in liposomes than in an aqueous solution (Fig. 3). In addition, as confirmed by IVIS measurement images, the concentration decreased as the color moved from red to blue, and it was confirmed that although the amount of remaining collagen decreased as the number of washes increased for both Example 1 and Comparative Example 1, the amount of remaining collagen in Example 1 was much higher than that in Comparative Example 1 (Fig. 4).

[0058] Experimental Example 3. Evaluation of Skin Penetration

[0059] To verify the degree of skin penetration using Example 2 and Comparative Example 2, in which the collagen content was increased by 1%, a skin penetration evaluation was conducted using 3D skin (Epi-200, Mattek). Specifically, 30 μL each of Example 2 and Comparative Example 2, containing collagen conjugated with the fluorescent reagent FITC, were added dropwise to a 3D skin containing only the epidermal layer, and the skin was incubated at 37°C for 4 hours. Afterward, the support to which the 3D skin was fixed was removed, and the separated 3D skin was placed in an OCT (Optimal Cutting Temperature) mold and stored at -80°C. Subsequently, the skin was sectioned into 20 μm pieces using a cryo-tissue sectioner. The sectioned tissues were observed using a confocal laser microscope, and the penetration amount was quantified using the Image J program.

[0060] As a result, Example 2 showed a higher fluorescence intensity than Comparative Example 2, confirming that skin permeability is improved when collagen is loaded into liposomes (Fig. 5).

[0062] Experimental Example 4. Comparative Evaluation of Skin Gene Expression Levels

[0063] To evaluate whether the efficacy actually exerted within the skin is enhanced as much as the skin permeability of collagen is improved, 30 μL each of Example 2 and Comparative Example 2 were applied to the 3D skin (Epi-200, Mattek) used in Experimental Example 3 for 4 hours, and after washing, the samples were cultured for 3 days to perform qRT-PCR (quantitative real-time polymerase chain reaction) on various skin-related genes. As a control for each gene expression level, the relative expression levels of the genes were compared based on GAPDH (Glyceraldehyde 3-phosphate dehydrogenase), which does not respond well to external conditions and is expressed evenly in most tissues.

[0064] As a result, it was confirmed that type 1 collagen, a gene related to collagen synthesis; keratin 5, keratin 14, and involucrin, which are related to keratinocyte differentiation; and aquaporin, a gene related to the stratum corneum dendrite, were all expressed more highly when treated with Example 2 (Fig. 6).

[0066] Experimental Example 5. Skin Safety Evaluation

[0068] * An evaluation of the skin safety of Example 2 above was performed. Specifically, the degree of irritation of Example 2 above was evaluated as follows on 20 adult men and women without skin diseases. After applying 20 µl of the sample to the forearm of the subjects, the test site was sealed and the patch was applied for 24 hours. After removing the patch, the skin reaction was examined 30 minutes and 24 hours later, respectively, according to the terminology presented in the CTFA guidelines. The Skin Irritation Index (PII) scores of the subjects obtained according to the judgment criteria were averaged and evaluated as low irritation if less than 1, mild irritation if less than 2, moderate irritation if less than 3.5, and strong irritation if 3.5 or higher.

[0069] Experimental items Example 2 Skin Irritation Index (PII) Non-irritating

[0071] As a result, as shown in Table 3 above, Example 2 was evaluated as non-irritating, confirming that it can be safely used in the product.

[0073] The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art will understand that other specific forms can be easily modified without altering the technical spirit or essential features of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive.

Claims

Claim 1 Oil phase containing lecithin, cholesterol, ceramide, and dipropylene glycol; A liposome composition having improved skin adhesion and absorption capacity of skin active substances, comprising an aqueous phase containing hydrolyzed collagen, wherein the lecithin is included in an amount of 0.1 to 1 weight part per 100 weight parts of the liposome composition, the cholesterol is included in an amount of 0.1 to 0.5 weight parts per 100 weight parts of the liposome composition, the ceramide is included in an amount of 0.005 to 0.1 weight part per 100 weight parts of the liposome composition, the dipropylene glycol is included in an amount of 1 to 20 weight parts per 100 weight parts of the liposome composition, the hydrolyzed collagen is included in an amount of 0.5 to 1 weight part per 100 weight parts of the liposome composition, the liposome has a particle size of 100 nm to 200 nm, and the liposome is prepared by the step of introducing the liposome into the aqueous phase after heating and melting the oil phase at 70 to 80°C. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 delete Claim 7 delete Claim 8 A liposome composition according to claim 1, wherein the liposome forms a multilayer structure. Claim 9 A cosmetic composition for improving skin wrinkles or enhancing elasticity, comprising a liposome composition of either claim 1 or claim 8 as an active ingredient.

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