Bacillus subtilis DJ-11 strain with high cellulolytic activity and food waste treatment composition comprising the same

Abstract

The present invention relates to microorganisms having cellulose degrading activity, and more specifically, to a novel strain of Bacillus subtilis DJ-11 having excellent cellulose degrading activity that can be used in food waste treatment equipment due to its excellent growth ability even at high temperatures and excellent cellulose degrading ability, and a food waste treatment composition containing the same.

Description

Technology Field

[0001] The present invention relates to a microorganism having cellulose degrading activity, and more specifically, to a novel strain of Bacillus subtilis having excellent cellulose degrading activity that can be used in food waste processing equipment, having excellent growth even at high temperatures and excellent cellulose degrading ability. Bacillus subtilis This invention relates to a DJ-11 strain and a food waste treatment composition containing the same. Background Technology

[0002] Food waste disposal methods can be classified into landfill, incineration, elimination through dehydration, drying, and fermentation, composting, and animal feed production. However, landfilling generates large amounts of leachate, contaminating groundwater and soil. Furthermore, it not only causes foul odors due to decay but also shortens the lifespan of landfill sites. In the case of incineration, the low calorific value and high moisture content lead to incomplete combustion, posing serious problems such as air pollution.

[0003] In the case of composting, it has failed due to crop damage because it has not been effective in the technical aspects of removing the stimulating properties of Korean food, particularly salt; similar problems exist in the field of animal feed production. Since simple drying methods and drying after fermentation have the disadvantages of requiring daily collection of leftover food from the dryer and consuming a large amount of energy during the drying process, the method of complete fermentation and decomposition using microorganisms can be considered the most ideal and efficient.

[0004] Meanwhile, vegetables, fruits, and grains containing cellulose account for 70–80% of food waste, posing a challenge to rapid composting through eco-friendly microbial decomposition. This is because cellulose has a complex structure, making its decomposition difficult. Cellulose decomposition is carried out by cellulose-degrading enzymes, which have been identified in nature, particularly in a broad spectrum of cellulose-degrading microorganisms (mainly fungi and bacteria), and exhibit high activity in wood-rotting bacteria and fungi.

[0005] Therefore, the development of strains with excellent cellulose-degrading capabilities is urgently required for the efficient processing of food waste containing large amounts of cellulose. Prior art literature

[0006] Republic of Korea Registered Patent No. 10-0424419 The problem to be solved

[0007] Accordingly, the objective of the present invention is to provide a novel strain, Bacillus subtilis DJ-11, which exhibits excellent proliferation and enzymatic activity at high temperatures, particularly in its ability to degrade cellulose.

[0008] Another objective of the present invention is to provide a food waste treatment composition suitable for treating food waste containing a large amount of cellulose by including Bacillus subtilis DJ-11, a microorganism that is non-pathogenic, has suitable enzymatic characteristics for general food physical and chemical conditions, namely salinity, acidity, and temperature, and particularly has excellent cellulose-degrading ability.

[0009] The objectives of the present invention are not limited to those mentioned above, and other unmentioned objectives will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0010] To achieve the objectives of the present invention as described above, the present invention provides a novel strain of Bacillus subtilis of accession number KACC 81253BP ( Bacillus subtilis) Provides the DJ-11 strain.

[0011] In a preferred embodiment, the cellulase activity of the strain is at least 2.5 times higher than that of the standard strain (KACC14549) when cultured at 45°C for 24 hours or more.

[0012] In a preferred embodiment, the strain has cellulase activity at pH 5 to 8 that is at least 1.3 times higher than that of the standard strain (KACC14549).

[0013] In a preferred embodiment, the strain exhibits a maximum growth rate at 10 wt% when cultured while changing the NaCl concentration to 3 to 12 wt%.

[0014] In a preferred embodiment, the strain exhibits a maximum growth rate at pH 8 when cultured while changing the pH to 5 to 8.

[0015] In addition, the present invention relates to Bacillus subtilis (B) of any one of the aforementioned accession numbers KACC 81253BP. acillus subtilis) DJ-11 strain and the above-mentioned Bacillus subtilis (B acillus subtilis) A food waste treatment composition comprising one or more culture solutions of the DJ-11 strain is provided.

[0016] In a preferred embodiment, the DJ-11 strain or culture medium is freeze-dried.

[0017] In a preferred embodiment, when the food waste is lettuce, more than 90% of the lettuce is decomposed by the DJ-11 strain when treated for 24 hours, and when the food waste is Chinese cabbage, more than 70% of the Chinese cabbage is decomposed by the DJ-11 strain when treated for 24 hours. Effects of the invention

[0018] Bacillus subtilis DJ-11 of the present invention exhibits excellent characteristics in terms of proliferation at high temperatures and enzymatic activity, particularly in its ability to decompose cellulose, and thus possesses characteristics suitable for use as a microorganism for food waste processing equipment.

[0019] Furthermore, the food dispersion treatment composition of the present invention is non-pathogenic and contains Bacillus subtilis DJ-11, a microorganism with suitable enzymatic characteristics for general food conditions, namely salinity, acidity, and temperature, and particularly excellent cellulose-degrading ability; thus, it is suitable for use in food waste treatment devices to treat food waste containing a large amount of cellulose.

[0020] The effects of the present invention are not limited to those mentioned above, and other unmentioned effects will be clearly understood by those skilled in the art from the description below. Brief explanation of the drawing

[0021] Figure 1 shows Bacillus subtilis (B acillus subtilis) This is the phylogenetic tree of the DJ-11 strain. Figure 2 shows Bacillus subtilis DJ cultured at 45°C. - This is a graph showing the growth curves over time of 11 strains and the standard strain (KACC 14549). Figure 3 shows Bacillus subtilis DJ1 cultured at 37°C and 45°C for 72 hours. - This is a photograph comparing the enzyme activity of 11 strains and the standard strain (KACC 14549). Figure 4 is a graph showing the growth and pH-related absorbance of the Bacillus subtilis DJ-11 strain. Figure 5 is a graph showing the growth and absorbance of the Bacillus subtilis DJ-11 strain in relation to NaCl concentration. Figure 6 is a result photograph showing the enzyme activity of Bacillus subtilis DJ-11 strain and standard strain (KACC 14549) according to NaCl concentration after 24 hours of culture (diameter measurement unit, mm). Figure 7 is a result photograph showing the enzyme activity of Bacillus subtilis DJ-11 strain and standard strain (KACC 14549) according to NaCl concentration after 48 hours of culture (diameter measurement unit, mm). Figure 8a is a result photograph showing the enzyme activity (diameter measurement unit, mm) of freeze-dried cultures obtained after culturing the Bacillus subtilis DJ-11 strain for 24 hours and 48 hours, respectively, and Figure 8b is a result photograph showing the enzyme activity after treating the freeze-dried cultures at 60°C and 70°C, respectively. Figures 9a and 9b are photographs of lettuce and Chinese cabbage used to verify the cellulose degradation ability of the Bacillus subtilis DJ-11 strain and the standard strain (KACC 14549), respectively. Specific details for implementing the invention

[0022] The terms used in this invention have been selected based on currently widely used general terms while considering their functions within the invention; however, these terms may vary depending on the intent of those skilled in the art, case law, the emergence of new technologies, etc. Additionally, in specific cases, terms have been selected at the applicant's discretion, and in such cases, their meanings will be described in detail in the relevant description of the invention.

[0023] In cases where terms such as 'includes,' 'has,' and 'is accomplished' mentioned in the present invention are used, other parts may be added unless 'only' is used. When a component is expressed in the singular, it includes cases where it is in the plural unless specifically stated otherwise.

[0024] In interpreting the components, they are interpreted to include a margin of error even in the absence of a separate explicit statement.

[0025] The characteristic parts of each of the various embodiments of the present invention may be combined or combined with one another, either partially or wholly, and may technically enable various interlocking and operation. Each embodiment may be implemented independently of one another or may be implemented together in an associated relationship.

[0026] Hereinafter, the technical configuration of the present invention will be described in detail with reference to the attached drawings and preferred embodiments.

[0027] However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Throughout the specification, the same reference numerals used to describe the present invention indicate the same components.

[0028] The technical features of the present invention include excellent proliferation and enzymatic activity capabilities at high temperatures, particularly the ability to decompose cellulose, and it is non-pathogenic. Since the enzymatic characteristics are suitable for the physicochemical conditions of general food, namely salinity, acidity, and temperature, Bacillus subtilis (B) is suitable for treating food waste containing a large amount of cellulose. acillus subtilis) It relates to the DJ-11 strain and its uses.

[0029] In other words, this is because while cellulose-degrading microorganisms are generally dominated by fungi, they are not suitable considering factors such as proliferation at high temperatures and pathogenicity; therefore, new cellulose-degrading microorganisms suitable for GRAS standards were isolated and identified.

[0030] Therefore, the present invention relates to a new strain of Bacillus subtilis ( Bacillus subtilis) Provides the DJ-11 strain (KACC81253BP).

[0031] Here, Bacillus subtilis DJ - 11(KACC81253BP) has the characteristic of having cellulase activity more than 2.5 times higher than the standard strain (KACC14549) when cultured at 45℃ for more than 24 hours.

[0032] In particular, Bacillus subtilis DJ - 11(KACC81253BP) showed cellulase activity at pH 5 to 8 that was more than 1.3 times higher than that of the standard strain (KACC14549), and exhibited a maximum growth rate at 10 wt% when cultured with NaCl concentrations changed from 3 to 12 wt%. In addition, it showed a maximum growth rate at pH 8 when cultured with pH changed from 5 to 8.

[0033] By utilizing the characteristics of Bacillus subtilis DJ-11 (KACC81253BP) of the present invention, a food waste treatment composition with a high cellulose content can be effectively manufactured.

[0034] As a result, the food waste treatment composition of the present invention is Bacillus subtilis DJ - One or more of 11 (KACC81253BP) and the culture medium of Bacillus subtilis DJ-11 (KACC81253BP) may be included as active ingredients.

[0035] If necessary, Bacillus subtilis DJ of the present invention - 11 (KACC81253BP) may be formulated into various forms for convenience, such as for transportation or storage. In particular, the Bacillus subtilis DJ-11 (KACC81253BP) strain or its culture solution may be used in powder form by freeze-drying it together with a cryoprotectant, for example, or it may be used by mixing it with a preservation carrier, adsorbing it, and then drying it to solidify it. There are no special restrictions on the cryoprotectant and preservation carrier as long as they are commonly used in the field; for example, glycerol, skim milk, honey, etc. may be used as the cryoprotectant, and diatomaceous earth, activated carbon, defatted starch, etc. may be used as the preservation carrier.

[0036] Examples

[0037] Bacillus subtilis (B acillus subtilis) The DJ-11 strain was isolated and identified as follows.

[0038] 1. Isolation of strains

[0039] Using the serial dilution plate method, the sample is mixed with distilled water (1 g sample / 10 ml D.W) and serially diluted, then plated on a selective medium containing prepared cellulose.

[0040] After plating, incubate at 50°C for 24 hours, and check for a clear zone to isolate only the cellulose-degrading bacteria. The isolated bacteria were cultured in pure culture.

[0041] 2. Identification of strains through 16S-rDNA sequencing

[0042] Subsequently, to identify the bacteria, genomic DNA was extracted (DNA purification kit, Promega) and sent to Geno Tech Corp. (Genotech) to amplify the 16S rDNA sequence of the isolated strain. Universal PCR primers 27F (5'-AGAGTTTGATCCTGGCT CAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3') were used. For 16S rDNA sequence analysis, the homology of the isolated strain was compared using the Blast Search program of Ezbiocloud (http: / / www.ezbiocloud.net / eztaxon) provided by ChunLab, Inc.

[0043] A neighbor-joining tree was constructed using the aligned nucleotide sequences via the neighbor-joining method, and the resulting phylogenetic tree is shown in Figure 1.

[0044] The strain selected from the above DNA sequence analysis results was identified as Bacillus subtilis. Bacillus subtilis It was named the DJ-11 strain, deposited with the National Institute of Agricultural Sciences Microbial Bank on February 17, 2023, and assigned the microorganism accession number (KACC 81253BP).

[0045] As shown in Fig. 1, Bacillus subtilis DJ-11 is Bacillus subtilis ( Bacillus subtilis ) subsp.inaquosorum strain BGSC 3A28 showed the closest relationship.

[0046] Experimental Example 1. Confirmation of growth potential of Bacillus subtilis DJ-11 strain according to temperature

[0047] To confirm the growth characteristics of Bacillus subtilis DJ-11 strain with accession number KACC 81253BP according to temperature, the following experiment was conducted. LB broth containing tryptone, yeast extract, and sodium chloride was sterilized at 121°C, 1.2 atm, and for 15 minutes, Bacillus subtilis DJ-11 strain and a standard strain (KACC 14549) were inoculated, respectively. The culture was shaken at 45°C and 180 rpm, which is the temperature at which the cellulase enzyme exhibits optimal activity, and absorbance was measured at 600 nm until the bacteria entered the death phase; the results are shown in Figure 2. At this time, KACC 14549, a strain commonly used to compare the mycological characteristics of Bacillus subtilis, was selected and used as the standard strain.

[0048] As shown in Fig. 2, the growth curve over 36 hours was confirmed through absorbance measurements, and the results showed maximum bacterial growth at 24 hours, followed by reaching the death phase. In particular Bacillus subtilis The DJ-11 strain exhibited high growth within 8 hours of inoculation into the medium, demonstrating significantly faster growth than the control standard strain; the maximum value reached by the standard strain after 24 hours of culture is compared to that of the present invention. Bacillus subtilis The DJ-11 strain was reached within 8 hours of culture. Through these results, the present invention Bacillus subtilis Since the DJ-11 strain grows at a rate more than three times faster than the standard strain, it can be seen that it has characteristics suitable for food waste processing equipment.

[0049] Experimental Example 2. Confirmation of Enzyme Activity of Bacillus subtilis DJ-11 Strain According to Temperature

[0050] A solid medium suitable for investigating cellulase activity was prepared, and then Bacillus subtilis DJ-11 strain and standard strain (KACC 14549) were inoculated using a loop and cultured in an incubator at 37°C and 45°C for 24 hours, 48 ​​hours, and 72 hours, respectively. The results are shown in Tables 1 and 2 and Figure 3.

[0051] Cellulase activity was measured using (CM-Cellulose 1%, Tryptone, Yeast extract, Sodium chloride, Agar 2%, Trypan blue 0.02%), and the diameter (unit: mm) was measured after confirming the clear zone. All media were sterilized at 121°C, 1.2 atm, for 15 minutes, and then solidified in petri dishes for use.

[0052] Culture time(hr) 24 48 72 DJ-11 17 21 35 KACC14549 (standard strain) 10 16 18

[0053] Culture time(hr) 24 48 72 DJ-11 23 29.5 40 KACC14549 (standard strain) 8 10 15

[0054] From Tables 1 and 2, as well as Figure 3, it can be seen that when cultured at 37°C and 45°C, the cellulase activity of the Bacillus subtilis DJ-11 strain was higher than that of the standard strain (KACC 14549), and in particular, when cultured at 45°C, the cellulase activity was more than 2.5 times higher than that of the standard strain (KACC 14549). In addition, although not presented as a specific experimental example, when the growth and enzyme activity of the Bacillus subtilis DJ-11 strain were confirmed after 24 hours of culture at higher temperatures, namely 50°C and 55°C, the cellulase activity was 16 mm at 50°C and 14.5 mm at 55°C.

[0055] From these results, it can be seen that the Bacillus subtilis DJ-11 strain of the present invention can exhibit excellent cellulase activity at a temperature of 45 to 55°C suitable for food processing.

[0056] Experimental Example 3. Confirmation of growth rate and enzyme activity of Bacillus subtilis DJ-11 strain according to pH

[0057] First, to confirm the growth of Bacillus subtilis DJ-11 strains according to pH, Bacillus subtilis DJ-11 strains were inoculated into liquid media adjusted to pH 4, 5, 6, 7, and 8 as follows, cultured, and the absorbance was measured, and the results are shown in Figure 4. At pH 4, Bacillus subtilis DJ-11 strains hardly grew, so it was not indicated.

[0058] Specifically, the pH of the liquid medium was adjusted to 4, 5, 6, 7, and 8 using a pH meter, and then the absorbance was measured at 600 nm after shaking incubation at 180 rpm at 45°C, which exhibits optimal enzyme activity. The experiment was repeated three times, and the average values ​​of the results were plotted on a graph. The liquid medium used was LB broth containing Tryptone, Yeast extract, and Sodium chloride, which was sterilized at 121°C, 1.2 atm, and 15 minutes.

[0059] As shown in Figure 4, the Bacillus subtilis DJ-11 strain showed the best bacterial growth at pH 8.

[0060] In addition, to investigate the enzyme activity of Bacillus subtilis DJ-11 strain and the standard strain (KACC 14549) according to pH, media were prepared with the respective substrates added as follows, inoculated, and cultured in a 45°C incubator for 24 and 48 hours, and the results are shown in Tables 3 and 4, respectively. Specifically, the pH of the solid media suitable for investigating each enzyme activity was adjusted to 4, 5, 6, 7, and 8 using a pH meter, the strains were inoculated using a loop, and then cultured in a 45°C incubator for 24 and 48 hours.

[0061] Cellulase activity was measured using (CM-Cellulose 1%, Tryptone, Yeast extract, Sodium chloride, Agar 2%, Trypan blue 0.02%), and the diameter (unit: mm) was measured after confirming the clear zone. The medium was sterilized at 121°C, 1.2 atm, for 15 minutes, and then solidified in a petri dish for use.

[0062] pH 5 6 7 8 DJ-11 38.5 40 52 53.5 KACC14549 (standard strain) 24 27 38 32

[0063] pH 5 6 7 8 DJ-11 43 47.5 56.5 57.5 KACC14549 (standard strain) 29 32 43 38

[0064] As shown in Figure 4, the optimal pH for the proliferation of the Bacillus subtilis DJ-11 strain of the present invention is 8, which is relatively high. However, it can be confirmed from the results in Tables 3 and 4 that the cellulose degradation activity of the Bacillus subtilis DJ-11 strain of the present invention exhibits an enzyme activity at least 1.3 times higher than that of the standard strain when cultured for 24 and 48 hours at a pH lower than 8, i.e., at pH 5 to 8.

[0065] Experimental Example 4. Confirmation of growth rate and enzyme activity of Bacillus subtilis DJ-11 strain according to NaCl concentration

[0066] First, to confirm the growth of Bacillus subtilis DJ-11 strains according to NaCl concentration, Bacillus subtilis DJ-11 strains were inoculated and cultured in liquid media with NaCl concentrations set to 3wt%, 5wt%, 7wt%, 8wt%, 10wt%, and 12wt% as follows, and the absorbance was measured, with the results shown in Figure 5. Specifically, the NaCl (wt%) of the liquid media was set to 3wt%, 5wt%, 7wt%, 8wt%, 10wt%, and 12wt%, and then the absorbance was measured at 600nm after shaking culture at 180rpm at 37℃ for 24 hours. The experiment was repeated three times, and the average values ​​of the results were plotted on a graph. The liquid media used was LB broth containing Tryptone, Yeast extract, and Sodium chloride. It was used after sterilization at 121℃, 1.2 atm, for 15 minutes, and the wt% was adjusted after excluding the sodium chloride previously included.

[0067] As shown in Figure 5, the Bacillus subtilis DJ-11 strain exhibited the greatest bacterial growth at 10 wt% and hardly grew at 12 wt%.

[0068] In addition, to investigate the enzyme activity of Bacillus subtilis DJ-11 strain and standard strain (KACC 14549) according to NaCl concentration, the samples were inoculated onto agar plates containing NaCl as follows and cultured in a 45°C incubator for 24 and 48 hours, and the results are shown in Figures 6 and 7, respectively. Specifically, the NaCl (w%) of the solid medium suitable for investigating each enzyme activity was adjusted to 3 wt%, 5 wt%, 8 wt%, 10 wt%, 12 wt%, and 15 wt%, and then the strains were inoculated using a loop and cultured in a 45°C incubator for 24 and 48 hours.

[0069] Cellulase activity was measured using (CM-Cellulose 1%, Tryptone, Yeast extract, Sodium chloride, Agar 2%, Trypan blue 0.02%), and the diameter (unit: mm) was measured after confirming the clear zone. All media were sterilized at 121°C, 1.2 atm, for 15 minutes, and then solidified in petri dishes for use.

[0070] From Figures 6 and 7, Bacillus subtilis DJ-11 strain exhibited cellulase activity up to concentrations of 8 wt%, 10 wt%, and 12 wt%, and broad clear zones were observed for each NaCl (wt%) compared to the standard strain. In particular, it was confirmed that enzyme activity was exhibited at a concentration of 12 wt% even though the bacteria did not grow.

[0071] Experimental Example 5. Confirmation of Enzyme Activity of Freeze-Dried Bacillus subtilis DJ-11 Culture Solution

[0072] To confirm whether the cellulose-degrading enzyme (cellulase) produced by the Bacillus subtilis DJ-11 strain of the present invention is an extracellularly secreted enzyme, the 48-hour culture of the Bacillus subtilis DJ-11 strain was centrifuged at 3,500 rpm for 20 minutes, the supernatant was filtered through a 0.25 µm membrane filter, and then freeze-dried. The freeze-dried enzyme powder, i.e., the freeze-dried material, was diluted 20-fold in a buffer solution and used. 20 µl was applied to a paper disc, placed on a solid medium to confirm enzyme activity, and incubated in a 45°C incubator for 24 and 48 hours. The diameter (unit: mm) was then measured to confirm enzyme activity, and the results are shown in Fig. 8a. In addition, to investigate enzyme activity according to temperature, freeze-dried samples were diluted 20-fold and treated in water baths at 60°C and 70°C for 30 minutes, respectively. Afterward, they were placed on solid media where enzyme activity could be verified via paper discs, incubated in a 37°C incubator for 24 and 48 hours, and the diameter (unit: mm) was measured to confirm the enzyme activity. The results are shown in Fig. 8b.

[0073] As shown in Fig. 8a, it was confirmed that the freeze-dried product of the Bacillus subtilis DJ-11 strain culture had enzymatic activity, but the cellulose degradation ability was weak compared to the activity of the isolated strain being proliferated.

[0074] As shown in Fig. 8b, it can be seen that the enzyme activity of the freeze-dried product of the Bacillus subtilis DJ-11 strain culture is maintained even at 70°C.

[0075] Experimental Example 6. Confirmation of weight loss rate of fiber-rich lettuce and cabbage upon treatment with Bacillus subtilis DJ-11 strain

[0076] To confirm the cellulose degradation ability of the Bacillus subtilis DJ-11 strain on fiber-rich lettuce and cabbage, lettuce and cabbage were cut mainly into leaf portions as shown in Figures 9a and 9b and fed into a food waste processor. Then, Bacillus subtilis DJ-11 (single strain application) and a control group (complex strain of the used strain) were inoculated and treated for 24 hours, after which the residue amount was checked. The reduction rate was calculated by comparing the residue amount with the input amount, and the results are shown in Table 5.

[0077] Materials / Strains Input amount (g) Residual amount (g / 24 hours) Weight loss rate (%) control group DJ-11 control group DJ-11 control group DJ-11 lettuce 1000 1000 360 100 64 90 napa cabbage 1000 1000 380 300 62 70

[0078] As shown in Table 5, when Bacillus subtilis DJ-11 (isolated strain) and existing food waste processing strains were applied to a food waste processor as a control group, the control group showed a reduction rate of 64% and 62%, respectively, after 24 hours of processing, whereas Bacillus subtilis DJ-11 of the present invention showed a reduction rate of 90% and 70%, confirming that Bacillus subtilis DJ-11 of the present invention exhibited a superior reduction in fiber, as seen in laboratory media.

[0079] From the above experimental results, it can be seen that the Bacillus subtilis DJ-11 strain of the present invention exhibits excellent cellulose decomposition ability not only at high temperatures but also in acidic environments with a salt concentration of 12 wt%. Therefore, it can be predicted that the food waste treatment composition containing the Bacillus subtilis DJ-11 strain of the present invention can rapidly process fiber-rich food by-products within a short period of time, thereby showing effectiveness in improving the rapid composting of fiber-rich food.

[0080] Although the present invention has been illustrated and described with preferred embodiments as described above, it is not limited to the embodiments described above, and various changes and modifications may be made by those skilled in the art within the scope of the invention without departing from the spirit of the invention.

[0081] Depository Name: National Institute of Agricultural Sciences, Rural Development Administration, Microbial Bank (KACC) Accession Number: KACC81253BP Date of Deposit: 2023-02-17

Claims

Claim 1 Bacillus subtilis of accession number KACC 81253BP ( Bacillus subtilis) DJ-11 strain. Claim 2 In claim 1, the strain is characterized by having a cellulase activity at least 2.5 times higher than that of the standard strain (KACC14549) when cultured at 45°C for 24 hours or more, Bacillus subtilis of accession number KACC 81253BP ( Bacillus subtilis ) DJ-11 strain. Claim 3 In claim 1, the strain is characterized by having cellulase activity at pH 5 to 8 that is at least 1.3 times higher than that of the standard strain (KACC14549), Bacillus subtilis (B) of accession number KACC 81253BP. acillus subtilis) DJ-11 strain. Claim 4 Bacillus subtilis of accession number KACC 81253BP (B), characterized in that, in claim 1, the strain exhibits a maximum growth rate at 10 wt% when cultured while changing the NaCl concentration to 3 to 12 wt%. acillus subtilis) DJ-11 strain. Claim 5 In claim 1, Bacillus subtilis (B) of accession number KACC 81253BP is characterized in that the strain exhibits a maximum growth rate at pH 8 when cultured while changing the pH from 5 to 8. acillus subtilis) DJ-11 strain. Claim 6 Bacillus subtilis of accession number KACC 81253BP (B) according to any one of claims 1 to 5 acillus subtilis) DJ-11 strain and the above-mentioned Bacillus subtilis (B acillus subtilis) A food waste treatment composition comprising one or more culture solutions of the DJ-11 strain. Claim 7 A food waste treatment composition according to claim 6, characterized in that the DJ-11 strain of accession number KACC 81253BP or its culture medium is freeze-dried. Claim 8 A food waste treatment composition according to claim 6, characterized in that when the food waste is lettuce, the lettuce is decomposed by more than 90% by the DJ-11 strain of accession number KACC 81253BP when treated for 24 hours, and when the food waste is Chinese cabbage, the Chinese cabbage is decomposed by more than 70% by the DJ-11 strain of accession number KACC 81253BP when treated for 24 hours.

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