Cultivation method of barley sprout with increased saponarin content by treatment of potassium and sodium

Abstract

The present invention relates to a method for increasing the saponarin content of barley sprouts, comprising the step of hydroponically cultivating sprouted barley by treating it with potassium and sodium nutrient solutions.

Description

Technology Field

[0001] The present invention relates to a method for cultivating barley sprouts with enhanced saponarin content through potassium and sodium treatment. Background Technology

[0002] Barley sprouts refer to young shoots grown to a height of approximately 10 to 20 cm by soaking barley grains and allowing them to germinate. Barley sprouts contain various vitamins, minerals, and enzymes, as well as SOD (superoxide dismutase), a powerful antioxidant enzyme. Furthermore, efficacy in blood pressure lowering, anti-inflammatory, antioxidant, anti-allergic, anti-ulcer, and anticancer effects has been reported due to the various functional components contained within them. The large amount of dietary fiber contained in barley sprouts is known to be effective in preventing chronic diseases such as hypertension, arteriosclerosis, and obesity by inhibiting the absorption of cholesterol and triglycerides. Additionally, a component called policosanol has been reported to increase levels of beneficial cholesterol (HDL cholesterol) while lowering levels of harmful cholesterol (LDL cholesterol). Moreover, because barley sprouts contain various functional secondary metabolites, their potential for industrial utilization as a material for health functional foods and pharmaceuticals is increasing. Recently, research results have been published indicating that barley sprouts contain a large amount of saponarin. According to these findings, 100 g of barley sprout powder contains up to 1,510 mg of saponarin. Saponarin is a type of flavonoid glycoside known for its excellent antioxidant activity and for exhibiting blood sugar-lowering, antibacterial, and hepatoprotective activities in animal studies.

[0003] Greenhouse cultivation refers to growing crops by artificially controlling the growing environment using artificial facilities such as glass greenhouses or vinyl greenhouses. It is also called facility horticulture because it generally involves the cultivation of horticultural crops such as vegetables, fruits, and flowers. Greenhouse cultivation has the advantage of enabling year-round and mass production by maintaining an environment suitable for crop growth through the artificial control of growing conditions—such as light, temperature, inducers, and moisture—using various auxiliary devices. Recently, the establishment of computer-based systems capable of controlling the cultivation environment optimized for each plant has contributed to increased farm income by enabling planned production and the cultivation of specialty crops.

[0004] Among facility cultivation methods for plants, hydroponics is a method of cultivating crops in a soilless environment without using soil, by fixing the crops in various ways and using a nutrient solution in which essential elements necessary for crop growth are dissolved at appropriate concentrations according to their absorption rates. It is also referred to as hydroponic cultivation, solution cultivation, or soilless cultivation. Hydroponics has the advantages of avoiding the continuous cropping problems associated with facility cultivation, facilitating scale expansion through equipment and mechanization, and enabling environmentally friendly agriculture due to the clean cultivation environment.

[0005] Artificial light sources primarily used in greenhouse plant cultivation include high-pressure sodium lamps, metal halide lamps, fluorescent lamps, and incandescent lamps; recently, the use of LEDs (Light Emitting Diodes) has been increasing. LEDs offer advantages such as low energy consumption, fixed wavelengths, long lifespan, low thermal radiation, and environmental protection. Furthermore, because they allow for precise control of light intensity and quality across a wide range, they are highly useful for maintaining optimized cultivation conditions tailored to specific plant species. However, for some plants, the high energy consumption required to maintain high-light conditions leads to increased operating costs in greenhouse cultivation. Therefore, there is a need for technological development regarding more economical and effective plant cultivation methods in greenhouses.

[0006] Meanwhile, Korean registered patent No. 2601904 discloses a 'method for increasing the saponarin content of barley sprouts using ethylene treatment' and Korean registered patent No. 2412033 discloses a 'method for cultivating barley sprouts with enhanced saponarin content by controlling light quality,' but there is no description of the method for cultivating barley sprouts with enhanced saponarin content by potassium and sodium treatment according to the present invention. The problem to be solved

[0007] The present invention was derived from the above-mentioned requirements, wherein the inventors treated germinated barley of various varieties with potassium and sodium nutrient solutions at 150 μmol / m² 2 Low light intensity conditions of / s or 500 μmol / m² 2After hydroponically culturing each under high light intensity conditions of / s, the saponarin content was analyzed, and it was confirmed that the saponarin content of the potassium and sodium nutrient solution treatment groups increased compared to the control group (distilled water treatment group). It was also confirmed that under low light intensity conditions, the saponarin content of the Hyeonuri variety increased by approximately 82% compared to the control group, and under high light intensity conditions, the saponarin content of the Keunalbori variety increased by approximately 84% compared to the control group, thereby completing the present invention. means of solving the problem

[0008] To solve the above problem, the present invention provides a method for cultivating barley sprouts with enhanced saponarin content, comprising the step of hydroponically cultivating germinated barley by treating it with a nutrient solution containing potassium and sodium.

[0009] In addition, the present invention provides barley sprouts with enhanced saponarin content cultivated by the above method.

[0010] In addition, the present invention provides a processed food containing the above-mentioned barley sprouts.

[0011] In addition, the present invention involves placing germinated barley on a hydroponic tray and treating it with a nutrient solution having a pH of 5–7 and containing 0.01–1.0% (w / v) potassium and 0.01–1.0% (w / v) sodium, and 100–200 μmol / m² 2 A method for increasing the saponarin content of barley sprouts is provided, comprising the step of hydroponic cultivation for 6 to 9 days under light intensity conditions of / s. Effects of the invention

[0012] The method of cultivating barley sprouts with enhanced saponarin content through potassium and sodium nutrient solution treatment according to the present invention has the effect of enabling the mass production of high-quality barley sprouts by identifying optimal nutrient solution treatment conditions for the production of barley sprouts with high saponarin content through facility cultivation. Specific details for implementing the invention

[0013] To achieve the objective of the present invention, the present invention provides a method for cultivating barley sprouts with enhanced saponarin content, comprising the step of hydroponically cultivating germinated barley by treating it with a nutrient solution containing potassium and sodium.

[0014] In the method for cultivating barley sprouts with enhanced saponarin content according to the present invention, the barley is preferably of the Hyenuri variety, but is not limited thereto.

[0015] In addition, the concentrations of potassium and sodium may preferably be 0.01 to 1.0% (w / v) each, more preferably 0.1 to 0.3% (w / v) for potassium and 0.05 to 0.15% (w / v) for sodium, and most preferably 0.2% (w / v) for potassium and 0.1% (w / v) for sodium, but are not limited thereto.

[0016] In addition, the above-mentioned nutrient solution containing potassium and sodium may preferably have a pH of 5 to 7, and more preferably a pH of 6, but is not limited thereto.

[0017] In addition, the above hydroponic cultivation may be performed under LED light conditions, preferably 100 to 200 μmol / m² 2 It may be performed under low light intensity conditions of / s, and more preferably 150 μmol / m² 2 It may be performed under low light conditions of / s, but is not limited thereto.

[0018] A method for cultivating barley sprouts according to one embodiment of the present invention specifically includes:

[0019] (a) a step of germinating Hyenuri barley seeds by soaking them in running water for 20 to 28 hours; and

[0020] (b) Place the germinated barley above on a hydroponic tray and treat with a nutrient solution containing 0.01–1.0% (w / v) potassium and 0.01–1.0% (w / v) sodium, and 100–200 μmol / m²2 It may include a step of hydroponic cultivation for 6 to 9 days under low light conditions of / s; and

[0021] More specifically,

[0022] (a) a step of germinating Hyenuri barley seeds by soaking them in running water for 24 hours; and

[0023] (b) The germinated barley was placed on a hydroponic tray and treated with a nutrient solution containing 0.2% (w / v) potassium and 0.1% (w / v) sodium, and 150 μmol / m² 2 It may include, but is not limited to, a step of hydroponic cultivation for 7 days under low light conditions of / s.

[0024] The present invention also provides barley sprouts with enhanced saponarin content cultivated by the above method.

[0025] The barley sprout with enhanced saponarin content according to the present invention is preferably of the Hyeonuri variety, and is characterized by an increase in saponarin content of approximately 82% compared to the control group without nutrient solution treatment by treatment with a nutrient solution containing potassium and sodium, and by treatment with a 0.2% (w / v) potassium and 0.1% (w / v) sodium nutrient solution to 150 μmol / m² 2 It is characterized by a saponarin content of 12,030 μg / g when cultivated under low light intensity conditions of / s. In addition, when treated with a nutrient solution containing 0.2% (w / v) potassium and 0.1% (w / v) sodium, 500 μmol / m² 2 When cultivated under high light intensity conditions of / s, the saponarin content increases by about 38% compared to the control group, so the saponarin content increases to the maximum when cultivated according to the method according to the present invention.

[0026] The present invention also provides a processed food containing the above-mentioned barley sprouts.

[0027] There are no specific restrictions on the types of the above-mentioned processed foods. Examples of foods to which the above-mentioned barley sprouts can be added include sauces, seasoned dishes, meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and include all processed foods in the conventional sense.

[0028] The present invention also comprises placing germinated barley on a hydroponic tray and treating it with a nutrient solution having a pH of 5–7 and containing 0.01–1.0% (w / v) potassium and 0.01–1.0% (w / v) sodium, and 100–200 μmol / m² 2 A method for increasing the saponarin content of barley sprouts is provided, comprising the step of hydroponic cultivation for 6 to 9 days under low light conditions of / s.

[0029] In the method for increasing the saponarin content of barley sprouts according to the present invention, the barley is preferably of the Hyeonuri variety, but is not limited thereto.

[0031] The present invention will be explained in detail below by way of examples. However, the following examples are merely illustrative of the present invention, and the scope of the present invention is not limited thereto.

[0033] Example 1. Hydroponic cultivation of barley sprouts using potassium (K) and sodium (Na) nutrient solution

[0034] 50 g of barley seeds (Keunalbori, Ssarirang, Ssarinuri, Hyemi, Hyeyang, Hyenuri) purchased from the National Institute of Food Science and Technology were immersed in running water for 24 hours to induce germination. Subsequently, the germinated barley was placed on a hydroponic tray, water was supplied, and LED white light at 150 μmol / m² was applied at 20℃. 2 Low light conditions of / s (photosynthetic photon flux density, PPFD) or 500 μmol / m² 2Each sample was cultivated under high light intensity conditions of / s(PPFD) for 24 hours. Barley seeds were positioned 30 cm below the light source. A nutrient solution was prepared by dissolving 2 g of K2HPO4 (>95%, Daejeong Chemical) and 1 g of NaCl (>95%, Daejeong Chemical) in 1 L of distilled water, and then adjusting the pH to 6 using 2 M HCl and 2 M NaOH aqueous solutions; this solution was then applied to each of the germinated barley samples. A group treated with distilled water without any additives was used as a control group. Seven days after treatment with the nutrient solution, the barley sprouts were harvested, dried at 45°C for 24 hours, and then ground using a mixer to produce dried samples, which were stored at -20°C for use in the experiment.

[0036] Example 2. Analysis of saponarin content in barley sprouts

[0037] 30 ml of 80% (v / v) methanol was added to 1 g of dried barley sprout sample and shaken for 24 hours. After centrifugation at 4,000 rpm for 10 minutes, 1 ml of the supernatant was taken and diluted 10-fold with 80% (v / v) methanol to prepare a sample for saponarin content analysis. The experiment was repeated three times for all samples.

[0038] The content of saponarin contained in barley sprouts was measured through HPLC (high-performance liquid chromatography) analysis using samples prepared by the above method. Standard calibration curves for quantification were prepared using saponarin standards from Extrasynthese at concentrations ranging from 0.5 to 50 mg / ℓ, and the analysis conditions are as shown in Table 1 below.

[0039]

[0041] As a result, it was confirmed that the saponarin content of the potassium and sodium nutrient solution treatment groups increased compared to the control group (distilled water treatment group). In particular, under low light conditions (150 μmol / m²) 2Under / s), it was confirmed that the saponarin content of the Hyeonuri variety increased by approximately 82% compared to the control group, and under high light intensity conditions (500 μmol / m²) 2 By confirming that the saponarin content of the Keunalbori variety increased by approximately 84% compared to the control group in / s), it was found that the type of barley variety in which saponarin content is maximized varies depending on the amount of light (Tables 2 and 3).

[0042] Analysis of Saponarin Content According to Nutrient Solution Treatment Under Low Light Conditions varieties Nutrient solution treatment Total saponarin content (µg / g) Barley sprout building material production (mg) dw / seed) Saponarin content (µg / seed) Growth rate (%) Big barley distilled water 7,039 10.5 73.6 43 K / Na solution 9,322 11.3 104.9 With sprouts distilled water 7,061 10.8 76.3 39 K / Na solution 9,794 10.9 106.3 Ssaknuri distilled water 5,791 10.9 63.1 50 K / Na solution 7,742 12.2 94.5 Hyemi distilled water 7,037 9.3 65.1 51 K / Na solution 10,022 9.8 98.2 Hyeyang distilled water 6,786 9.7 65.5 62 K / Na solution 10,089 10.5 105.9 Hyenuri distilled water 8,101 7.7 62.4 82 K / Na solution 12,030 9.5 113.7

[0044] Analysis of Saponarin Content According to Nutrient Solution Treatment Under High Light Intensity Conditions varieties Nutrient solution treatment Total saponarin content (µg / g) Barley sprout building material production (mg) dw / seed) Saponarin content (µg / seed) Growth rate (%) Big barley distilled water 8,350 13.8 114.8 84 K / Na solution 13,601 15.6 211.5 With sprouts distilled water 9,811 13.9 136.4 50 K / Na solution 13,695 15.0 204.7 Ssaknuri distilled water 7,171 13.8 99.0 64 K / Na solution 9,697 16.7 161.9 Hyemi distilled water 9,800 13.9 136.2 39 K / Na solution 13,144 14.4 189.3 Hyeyang distilled water 9,281 12.6 116.9 44 K / Na solution 12,259 13.7 167.9 Hyenuri distilled water 10,994 10.4 114.3 38 K / Na solution 14,979 10.5 157.3

Claims

Claim 1 (a) a step of germinating Hyenuri barley seeds by immersing them in running water for 20 to 28 hours; and (b) placing the germinated barley on a hydroponic tray and treating it with a nutrient solution having a pH of 5 to 7 and containing 0.01 to 1.0% (w / v) potassium and 0.01 to 1.0% (w / v) sodium, and 100 to 200 μmol / m² 2 A method for cultivating barley sprouts with enhanced saponarin content, comprising the step of hydroponic cultivation for 6 to 9 days under low light conditions of / s. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 delete Claim 7 delete Claim 8 delete Claim 9 Germinated Hyeonuri barley was placed in a hydroponic tray and treated with a nutrient solution containing 0.01–1.0% (w / v) potassium and 0.01–1.0% (w / v) sodium with a pH of 5–7, and 100–200 μmol / m² 2 A method for increasing the saponarin content of barley sprouts, comprising the step of hydroponic cultivation for 6 to 9 days under low light conditions of / s.

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