Composition for preventing or improving skin wrinkle comprising extract of Cirsium setidens cultivated in smart farm as effective component

Abstract

The present invention relates to a composition for preventing or improving skin wrinkles containing a smart farm-grown Gondre extract as an active ingredient. Since the smart farm-grown Gondre extract of the present invention has a significantly superior effect in preventing or improving skin wrinkles caused by ultraviolet irradiation compared to an open-field-grown Gondre extract, it can be usefully used as a cosmetic and health functional food for preventing or improving skin wrinkles caused by ultraviolet rays.

Description

Technology Field

[0001] The present invention relates to smart farm cultivated Gondeure ( Cirsium setidens This invention relates to a composition for preventing or improving skin wrinkles containing an extract as an active ingredient. Background Technology

[0002] The skin is the outermost organ of the body and is an important organ that has various functions and significantly influences appearance and image. It consists of various cells and a unique structure that covers the outer surface of the body, serving as the body's primary defense barrier by preventing moisture evaporation, regulating body temperature, protecting against external stimuli, and preventing the intrusion of harmful microorganisms.

[0003] The skin can be damaged by various external factors, including sunlight. Sunlight consists of visible light, ultraviolet (UV) rays, and infrared rays. Among these, UV rays play beneficial roles, such as synthesizing Vitamin D in the body and sterilizing, but they can also cause skin aging, skin cancer, dryness, dermatitis, fine lines, melasma, and freckles. UV rays are classified into three types—A, B, and C—based on wavelength; however, UVC is blocked by the ozone layer, and only UVA and UVB affect the skin. In particular, UVB (290–320 nm), a medium-wavelength UV ray, is known to cause many side effects, including the development of skin cancer, suppression of the immune system, and photoaging.

[0004] Meanwhile, Gondre ( Cirsium setidens It is a perennial herb belonging to the Asteraceae family and is widely distributed not only in East Asia but also along the Mediterranean coast and in the southwestern regions of North America, ranging from temperate to frigid zones. Its young leaves and stems are edible; as a wild vegetable containing various nutrients such as minerals and vitamins, it was used as an important famine food in mountainous regions during times of food scarcity in the past.

[0005] Meanwhile, a smart farm is a specialized facility introduced to agricultural sites as part of efforts to improve productivity and innovate agriculture. It integrates information and communication technology (ICT) into agricultural production, processing, distribution, and consumption to automatically manage crop growth environments and, furthermore, to enhance production efficiency. Additionally, its purpose is to cultivate top-quality plants by creating an environment necessary for plant growth within a closed space and continuously supplying nutrient-rich water.

[0006] Meanwhile, Korean registered patent No. 1800608 discloses 'a composition for lowering blood sugar using a gondre extract as an active ingredient and a method for manufacturing the same,' and Korean registered patent No. 2942275 discloses 'a composition for improving, preventing, or treating depressive symptoms, stress, or cognitive impairment containing a gondre extract as an active ingredient,' but there is no description yet of a composition for preventing or improving skin wrinkles containing a smart farm-grown gondre extract of the present invention as an active ingredient. The problem to be solved

[0007] The present invention was derived from the above-mentioned requirements. The present invention relates to a composition for preventing or improving skin wrinkles containing smart farm-grown Gondre extract as an active ingredient. The invention was completed by confirming that the MMP-1 activity inhibitory effect was excellent when human skin fibroblasts were pretreated with smart farm-grown Gondre extract and then irradiated with ultraviolet rays. means of solving the problem

[0008] To solve the above problem, the present invention provides a cosmetic composition for preventing or improving skin wrinkles containing smart farm-grown Gondeure extract as an active ingredient.

[0009] In addition, the present invention provides a health functional food composition for preventing or improving skin wrinkles containing smart farm-grown Gondeure extract as an active ingredient. Effects of the invention

[0010] The present invention relates to a composition for preventing or improving skin wrinkles containing a smart farm-grown Gondre extract as an active ingredient. Since the smart farm-grown Gondre extract of the present invention has a significantly superior effect in preventing or improving skin wrinkles caused by ultraviolet irradiation compared to an open-field-grown Gondre extract, it can be usefully used as a cosmetic and health functional food for preventing or improving skin wrinkles caused by ultraviolet rays. Brief explanation of the drawing

[0011] Figure 1 shows the results of analyzing cell viability after treating human skin keratinocyte HaCaT cells with open-field cultivated Gondeure extract (A) or smart-farm cultivated Gondeure extract (B). N: Untreated group. *, ***: vs N. Figure 2 shows the results of measuring the MMP-1 (matrix metalloproteinase-1) content in the cell culture medium after pre-treating human skin keratinocyte HaCaT cells with open-field cultivated Gondeure extract or smart-farm cultivated Gondeure extract and irradiating with ultraviolet B (UVB). UVB: Ultraviolet B, N: Untreated group, NC: UVB alone treated group, PC: 1 mM NAC (N-Acetyl L-cysteine) treated group. ***: vs N. ###: vs NC. ￥: vs open-field cultivated Gondeure extract. Specific details for implementing the invention

[0012] The present invention provides a cosmetic composition for preventing or improving skin wrinkles containing smart farm-grown Gondeure extract as an active ingredient.

[0013] In the cosmetic composition for preventing or improving skin wrinkles according to the present invention, the skin wrinkles may be skin wrinkles caused by ultraviolet rays, preferably skin wrinkles caused by ultraviolet B rays, but are not limited thereto.

[0014] In the cosmetic composition for preventing or improving skin wrinkles according to the present invention, the extraction solvent of the smart farm-grown Gondre extract may be a C1 to C4 lower alcohol, water, or a mixture thereof, preferably ethanol, but is not limited thereto.

[0015] In addition, the smart farm cultivated Gondre extract may be prepared using conventional methods known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction, and preferably, it may be prepared by ultrasonic extraction at 60 to 80°C for 2 to 4 hours, but is not limited thereto.

[0016] In the cosmetic composition for preventing or improving skin wrinkles according to the present invention, the smart farm-grown Gondeure extract is characterized by having an excellent inhibitory effect on the synthesis of MMP-1, and in particular, is characterized by having a significantly superior inhibitory effect on the synthesis of MMP-1 compared to open-field-grown Gondeure.

[0017] In a cosmetic composition for preventing or improving skin wrinkles according to the present invention, the smart farm-cultivated Gondeure is preferably Gondeure seedlings transplanted into a hydroponic cultivation bed containing nutrient solution, and then an LED (Light Emitting Diode) light source is used having a temperature of 22–24°C, a relative humidity of 60–75%, and a ratio of blue light:red light:white light of 4–6:2–4:1–3, and a Photosynthetic Photon Flux Density (PPFD) of 80–100 μmol / m² 2 It may be Gondre cultivated for 3 to 5 weeks in a smart farm maintained at / s, and more preferably, after transplanting Gondre seedlings into a hydroponic bed containing nutrient solution, an LED (Light Emitting Diode) light source is used with a temperature of 22–24℃, relative humidity of 60–75%, and a blue:red:white light ratio of 5:3:2, and a Photosynthetic Photon Flux Density (PPFD) of 90 μmol / m²2 It may be Gondeure grown for 4 weeks in a smart farm maintained at / s, but is not limited thereto.

[0018] In a cosmetic composition according to one embodiment of the present invention, the cosmetic composition for preventing or improving skin wrinkles may have any one formulation selected from the group consisting of cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, nourishing essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives that are necessary and appropriate for the formulation of the formulation, and the types and amounts of these ingredients can be easily selected by a person skilled in the art.

[0019] In the case where the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0020] When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane-butane, or dimethyl ether.

[0021] In the case where the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvating agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0022] In the case where the formulation of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used as carrier components.

[0023] In the case where the formulation of the cosmetic composition of the present invention is a cleansing product containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0024] The cosmetic composition of the present invention may additionally contain excipients including fluorescent substances, fungicides, hydrotropism-inducing substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunblocks, vitamins, plant extracts, etc.

[0025] The present invention also provides a health functional food composition for preventing or improving skin wrinkles containing smart farm-grown Gondeure extract as an active ingredient.

[0026] In the health functional food composition for preventing or improving skin wrinkles of the present invention, the smart farm cultivated Gondeure extract is as described above.

[0027] When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as is or used together with other foods or food ingredients, and may be used appropriately according to conventional methods. The amount of the active ingredient may be appropriately used according to its intended use (prevention or improvement). Generally, when manufacturing food or beverages, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, relative to the raw material. However, in the case of long-term consumption for health purposes, the above amount may be less than the above range, and since there are no issues regarding safety, the active ingredient may be used in an amount greater than the above range.

[0028] There are no special restrictions on the types of the above-mentioned health functional foods. Examples of foods to which the above-mentioned health functional food composition may be added include meat, sausage, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea drinks, alcoholic beverages, and vitamin complexes, and include all health foods in the conventional sense.

[0029] In addition, the health functional food composition of the present invention may be manufactured into a food, particularly a functional food. The functional food of the present invention includes ingredients that are typically added during food manufacturing, such as proteins, carbohydrates, fats, nutrients, and flavoring agents. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredients. The natural carbohydrate is preferably a monosaccharide (e.g., glucose, fructose, etc.), a disaccharide (e.g., maltose, sucrose, etc.), an oligosaccharide, a polysaccharide (e.g., dextrin, cyclodextrin, etc.), or a sugar alcohol (e.g., xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., taumatin, stevia extract, etc.) or a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

[0030] In addition to the above health functional food composition, it may further contain various nutritional agents, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. Although the proportion of these added ingredients is not of great importance, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health functional food composition of the present invention.

[0032] The present invention will be explained in detail below through examples. However, the following examples are merely illustrative of the present invention, and the scope of the present invention is not limited to the following examples.

[0034] Example 1. Cultivation of Gondre in a Smart Farm and Preparation of Extract

[0035] 1-1. Cultivating Gondre in Smart Farms

[0036] Gondre seeds were sown in potting soil and germination was induced and seedlings were grown in a smart farm where conditions of a temperature of 21–23°C and a relative humidity of 70–85% were maintained, yielding seedlings with a leaf length of approximately 1–2 cm and a root length of approximately 2 cm or more. The seedlings were transplanted into a hydroponic bed containing a nutrient solution (Daeyu Mulpuré No. 1, 50 ml / 20 L), and then cultivated in a smart farm where conditions of a photosynthetic photon flux density (PPFD) of 90 μmol / m² / s were maintained for 16 hours a day, with blue, red, and white LEDs (Light emitting diodes) combined in a ratio of 5:3:2 under conditions of a temperature of 22–24°C and a relative humidity of 60–75%. After 4 weeks of transplanting to a hydroponic bed, Gondeure with a leaf length of about 5 cm and a root length of about 12 cm or more was harvested and subsequently used to produce extracts.

[0038] 1-2. Production of Gondre Extract Cultivated in Smart Farms

[0039] The stems and leaves of Gondeure harvested by cultivating using the above method were freeze-dried and powdered, then 10 times the amount of 70% (v / v) ethanol was added and ultrasonic extraction was performed at 70°C for 3 hours. Afterward, the mixture was vacuum-filtered using a 110 mm diameter filter paper, the solvent was removed using a rotary evaporator, and the resulting freeze-dried material was used for subsequent experiments. Gondeure seeds were sown in an open field and cultivated for approximately 4 to 6 weeks (leaf length approximately 5 cm, root length approximately 12 cm or more), and then extracted using the same method as above and used as a control group.

[0041] Example 2. Component Analysis

[0042] The content of pectolinarin in open-field cultivated Gondeure extract and smart farm cultivated Gondeure extract was measured. Specifically, an HPLC-DAD [Kinetex 5 μm C18 100 A, LC Column 250×4.6 mm] instrument was used, and the mobile phase consisted of 0.05% phosphoric acid solution (phosphoric acid in water) and acetonitrile. 15 μl of each sample was injected at a flow rate of 0.6 ml / min, and measurements were taken at a wavelength of 330 nm.

[0043] As a result, it was confirmed that the pectolinarin content of smart farm-grown Gondre extract was higher than that of open-field-grown Gondre extract (Table 1).

[0044] Component analysis results Ingredient name Smart farm cultivated Gondre extract Open-field cultivated Gondre extract pectolinarin (mg / g) 48.1 38.2

[0046] Example 3. Cytotoxicity Analysis

[0047] The cytotoxicity of Gondre extract was analyzed using HaCaT cells, which are human skin keratinocytes. HaCaT cells (Cytion, 300493) were cultured in DMEM (Dulbecco's modified Eagle medium) supplemented with 10% (v / v) FBS (fetal bovine serum) and 1% (v / v) penicillin (100 U / mL) / streptomycin (100 µg / mL) at 37°C under 5% CO2 conditions, and then transferred to a 96-well plate containing 2×10⁴ cells. 4 Cells were dispensed into wells and cultured for 24 hours. Subsequently, the smart farm-cultivated Gondeure extract according to the present invention was diluted to concentrations of 1, 10, 100, 200, 400, or 1,000 μg / ml and treated for 24 hours, after which cell viability was calculated using a cell proliferation assay kit (CellTiter 96 AQueous One Solution Cell Proliferation Assay, Promega). The open-field cultivated Gondeure extract treatment group was used as the control group.

[0048] As a result, it was confirmed that the cell viability of the open-field cultivated Gondre extract treatment group decreased significantly starting from a concentration of 100 μg / ml, whereas the cell viability of the smart farm cultivated Gondre extract treatment group remained at approximately 100% up to a concentration of 1,000 μg / ml (Fig. 1).

[0050] Example 4. Analysis of the skin wrinkle improvement effect caused by ultraviolet rays

[0051] 4×10 HaCaT cells in a 6-well plate 5 After dispensing to form cells / wells and culturing for 36 hours, the smart farm-cultivated Gondeure extract according to the present invention was diluted to concentrations of 10, 25, or 50 μg / ml and pretreated for 24 hours, followed by washing twice with PBS, and ultraviolet B (UVB) irradiation was performed using a UV cross linker (Analytik Jena, Germany) at 15 mJ / cm² 2 After investigation, the medium was exchanged for serum-deficient DMEM and cultured for 48 hours.

[0052] Subsequently, to analyze the effect of smart farm-grown Gondre extract on improving skin wrinkles caused by ultraviolet rays, the MMP-1 (matrix metalloproteinase-1) content was measured. Specifically, cell culture medium was recovered, and the MMP-1 content was measured using an MMP-1 ELISA kit (Abcam, USA). The treatment group with no treatment was used as the normal group, the open-field grown Gondre extract treatment group was used as the comparison group, and the 1 mM NAC (N-Acetyl L-cysteine) treatment group was used as the positive control.

[0053] As a result, it was confirmed that the MMP-1 content of the group treated with UVB alone increased significantly compared to the normal group, whereas the MMP-1 content of the group treated with smart farm-grown Gondeure extract decreased significantly compared to the group treated with UVB alone. In particular, it was confirmed that the MMP-1 content of the group treated with smart farm-grown Gondeure extract at concentrations of 25 or 50 μg / ml decreased significantly compared to the group treated with open-field-grown Gondeure extract at the same concentration (Fig. 2).

[0054] Based on the above results, it was found that the skin wrinkle improvement effect caused by ultraviolet rays of the Gondre extract grown in a smart farm by the method according to the present invention was significantly superior compared to the Gondre extract grown in an open field.

Claims

Claim 1 A cosmetic composition for preventing or improving skin wrinkles caused by ultraviolet rays, comprising an ethanol extract of smart farm-cultivated Gondeure as an active ingredient, wherein the smart farm-cultivated Gondeure is obtained by transplanting Gondeure seedlings into a hydroponic bed, using an LED (Light Emitting Diode) light source with a temperature of 22–24°C, a relative humidity of 60–75%, and a ratio of blue light:red light:white light of 5:3:2, and a Photosynthetic Photon Flux Density (PPFD) of 90 μmol / m² 2 A cosmetic composition for preventing or improving skin wrinkles caused by ultraviolet rays, characterized in that the gondre is cultivated for 4 weeks in a smart farm maintained at / s, the smart farm-cultivated gondre ethanol extract inhibits the synthesis of MMP-1, and the pectolinarin content of the smart farm-cultivated gondre ethanol extract is 48.1 mg / g. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 A cosmetic composition for preventing or improving skin wrinkles caused by ultraviolet rays, wherein, in claim 1, the cosmetic composition is prepared in any one formulation selected from the group consisting of skin, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, eye cream, moisture cream, hand cream, essence, nourishing essence, pack, cleansing foam, cleansing water, cleansing lotion, cleansing cream, body lotion, body cleanser, soap, and powder. Claim 7 A health functional food composition for preventing or improving skin wrinkles caused by ultraviolet rays, comprising an ethanol extract of smart farm-cultivated Gondeure as an active ingredient, wherein the smart farm-cultivated Gondeure is obtained by transplanting Gondeure seedlings into a hydroponic bed, using an LED (Light Emitting Diode) light source with a temperature of 22–24°C, a relative humidity of 60–75%, and a ratio of blue light:red light:white light of 5:3:2, and a Photosynthetic Photon Flux Density (PPFD) of 90 μmol / m² 2 A health functional food composition for preventing or improving skin wrinkles caused by ultraviolet rays, characterized in that the gondre is cultivated for 4 weeks in a smart farm maintained at / s, the smart farm-cultivated gondre ethanol extract inhibits the synthesis of MMP-1, and the pectolinarin content of the smart farm-cultivated gondre ethanol extract is 48.1 mg / g.

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