Composition for Immunity Enhancement comprising Cudrania Extract and Red Ginseng Extract

Abstract

The present invention relates to an immune-enhancing composition comprising Cudrania tricuspidata extract and red ginseng extract. Since it exhibits excellent improvement effects on reduced spleen weight and serum immunoglobulin concentration without side effects such as weight loss and decreased appetite, it can be effectively utilized for immune enhancement purposes.

Description

Technology Field

[0001] The present invention relates to an immune-enhancing composition comprising Cudrania tricuspidata extract and red ginseng extract.

[0002] This invention was made with support from the Chungbuk Technopark Oriental Medicine and Natural Products Center, under the research project "Development of Functional Foods with Immunity-Boosting Labels Using Cudrania tricuspidata Fruit and Red Ginseng" of the "2023 Chungcheongbuk-do Immune System Enhancement Product Development Support Project" (Research period: 2023.01.01. ~ 2023.11.30.). Background Technology

[0003] Immunity refers to a series of biological defense reactions that occur to exclude substances other than its own components that disrupt the body's homeostasis or threaten it. It is a defense system that protects the body from harmful substances that invade through the skin, digestive tract, respiratory tract, etc., and can be described as an activity that maintains homeostasis through phagocytosis to remove external irritants or by reducing severe inflammation in wounds.

[0004] If immune function is deficient or impaired, the immune response may not be properly activated, leading to an inability to react effectively to foreign substances in the body and potentially causing infections. Furthermore, various immune diseases such as asthma, seasonal or perennial rhinitis, allergic rhinitis, conjunctivitis, atopic dermatitis, urticaria, hemolysis of red blood cells, acute glomerulonephritis, the common cold, chronic fatigue, and cancer may occur.

[0005] Recently, as concerns regarding the side effects of synthetic compounds have emerged, there is a demand for the development of materials from natural sources that can effectively induce immune-enhancing effects. Furthermore, the need for the development of novel immune-enhancing materials is on the rise following the COVID-19 pandemic.

[0006] Meanwhile, the scientific name of the Cudrania tricuspidata is Cudrania tricuspiata(Carr.) Bureau ex Lavallee belongs to the Moraceae family and is a versatile tree with no waste, as everything from the fruit to the leaves, roots, and stems is edible. Red ginseng is a processed ginseng product made by steaming and drying ginseng, and it is known that various new physiologically active components beneficial to the body are generated during the manufacturing process of red ginseng.

[0007] Accordingly, the inventors of the present invention completed the present invention by conducting research to develop an effective immune-enhancing material utilizing Cudrania tricuspidata and red ginseng. Prior art literature

[0008] Republic of Korea Patent Publication No. 10-2011-0026570 The problem to be solved

[0009] One objective of the present invention is to provide a health functional food for immune enhancement comprising Cudrania tricuspidata extract and red ginseng extract.

[0010] Another objective of the present invention is to provide a pharmaceutical composition for immune enhancement comprising Cudrania tricuspidata extract and red ginseng extract. means of solving the problem

[0011] One aspect of the present invention provides a health functional food for immune enhancement comprising Cudrania tricuspidata extract and red ginseng extract.

[0012] The extract included in the health functional food according to the present invention can be obtained as follows. Red ginseng or Cudrania tricuspidata is washed with water to remove foreign substances and then dried in the shade. An appropriate amount of solvent is added to each of the red ginseng or Cudrania tricuspidata to ensure complete immersion. At this time, the red ginseng or Cudrania tricuspidata may be used in its dried state or ground into a powder form. Red ginseng or Cudrania tricuspidata can be extracted using a conventional extraction solvent, and preferably, extracted using (a) anhydrous or aqueous lower alcohol having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, and normal-butanol, etc.), (b) a mixed solvent of the lower alcohol and water, (c) acetone, (d) ethyl acetate, (e) chloroform, (f) 1,3-butylene glycol, (g) hexane, (h) diethyl ether, (i) butyl acetate, (j) chloroform-methanol, or (k) water, and more preferably extracted using water, ethanol, or an aqueous ethanol solution. During extraction, impregnation at room temperature or heating may be performed, and the product may be prepared as a freeze-dried powder.

[0013] According to one embodiment of the present invention, the extract may be extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

[0014] The health functional food of the present invention may include ingredients that are typically added during the manufacture of health functional foods, and may include, for example, proteins, carbohydrates, fats, nutrients, seasonings, and flavorings. Examples of carbohydrates may be monosaccharides, e.g., glucose, fructose, etc.; disaccharides, e.g., maltose, sucrose, oligosaccharides, etc.; and polysaccharides, e.g., conventional sugars such as dextrin, cyclodextrin, etc., and sugar alcohols such as xylitol, sorbitol, erythritol, etc. As flavorings, natural flavorings [taumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.)] and synthetic flavorings (saccharin, aspartame, etc.) may be used.

[0015] For example, when the health functional food of the present invention is manufactured as a drink, in addition to the composition of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, Eucommia ulmoides extract, jujube extract and / or licorice extract may be additionally included.

[0016] In addition, the health functional food of the present invention may contain various nutritional supplements, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc.

[0017] These ingredients may be used independently or in combination, and the proportion of these additives may be selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the health functional food of the present invention, but is not limited thereto.

[0018] According to one embodiment of the present invention, the weight ratio of the Cudrania tricuspidata extract and the red ginseng extract may be 7:3 to 9:1.

[0019] If the red ginseng extract exceeds 3 weight parts based on 7 weight parts of Cudrania tricuspidata extract, side effects such as weight loss may occur and the immune-enhancing effect may be low. On the other hand, if the red ginseng extract is less than 1 weight part based on 9 weight parts of Cudrania tricuspidata extract, a synergistic effect for immune enhancement may not be sufficiently observed. Therefore, the weight ratio of Cudrania tricuspidata extract and red ginseng extract of the present invention is preferably 7:3 to 9:1, and more preferably 8:2.

[0020] According to one embodiment of the present invention, the Cudrania tricuspidata may be a Cudrania tricuspidata fruit.

[0021] For the immune-enhancing effect, the Cudrania tricuspidata extract of the present invention is preferably a Cudrania tricuspidata fruit extract.

[0022] According to one embodiment of the present invention, the immune enhancement may be due to an increase in the concentration of IgM in the serum.

[0023] When the weight ratio of the Cudrania tricuspidata extract and the red ginseng extract of the present invention is 8:2, a synergistic effect is observed in increasing the concentration of immunoglobulins in the serum, particularly IgM. Therefore, the immune-enhancing health functional food of the present invention can be effectively utilized for people whose immune function has decreased due to low IgM concentrations in the serum.

[0024] Another aspect of the present invention provides a pharmaceutical composition for immune enhancement comprising Cudrania tricuspidata extract and red ginseng extract.

[0025] The composition of the present invention may include a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier included in the composition of the present invention is one commonly used in the manufacture of pharmaceuticals and includes, but is not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition to the above components, the pharmaceutical composition of the present invention may further include lubricants, humectants, sweeteners, flavorings, emulsifiers, suspending agents, preservatives, etc. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington: the science and practice of pharmacy 22nd edition (2013).

[0026] A pharmaceutical composition according to one embodiment of the present invention may be administered together with one or more substances exhibiting immunostimulatory activity.

[0027] In addition, a pharmaceutical composition according to one embodiment of the present invention may be used alone or in combination with methods using procedures, hormone therapy, drug therapy and / or biological response modulators for immune enhancement.

[0028] The composition of the present invention may include various bases and / or additives that are necessary and appropriate for the formulation of the formulation, and may be prepared by further including known compounds such as nonionic surfactants, silicone polymers, extender pigments, fragrances, preservatives, fungicides, oxidation stabilizers, organic solvents, ionic or nonionic thickeners, emollients, antioxidants, free radical destroyers, opacifiers, stabilizers, emollients, silicones, α-hydroxy acids, defoaming agents, moisturizers, vitamins, insect repellents, fragrances, preservatives, surfactants, anti-inflammatory agents, substance P antagonists, fillers, polymers, propellants, basicizing or acidifying agents, or coloring agents, to the extent that the effect is not reduced.

[0029] The effective dosage of the composition of the present invention to the human body may vary depending on the patient's age, body weight, gender, form of administration, health condition, and degree of disease, and is generally about 0.001 to 1000 mg / kg / day, preferably 0.1 to 500 mg / kg / day, and may be administered in divided doses once or several times a day at regular intervals according to the judgment of a doctor or pharmacist.

[0030] The composition of the present invention can be administered orally.

[0031] The composition of the present invention may be administered in various formulations when administered orally, such as tablets, pills, hard / soft capsules, liquids, suspensions, emulsifiers, syrups, granules, elixirs, troches, etc., and may further include various excipients, such as humectants, sweeteners, flavorings, preservatives, etc. Specifically, when the composition of the present invention is formulated into an oral administration formulation, it may further include suitable carriers, excipients, and diluents commonly used in its manufacture. The above carrier, excipient, and diluent may include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and / or mineral oil. Additionally, it may be prepared by including diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are commonly used in formulations, and may further include a lubricant such as magnesium stearate or talc in addition to the above excipients. Effects of the invention

[0032] According to an immune-enhancing composition containing Cudrania tricuspidata extract and red ginseng extract, it exhibits excellent improvement effects on reduced spleen weight and serum immunoglobulin concentration without side effects such as weight loss and decreased appetite, and can be effectively utilized for immune enhancement purposes. Brief explanation of the drawing

[0033] Figure 1 is a photograph showing red ginseng concentrate diluted with distilled water and frozen. Figure 2 is a photograph showing the freeze-dried powder of the hot water extract of dried Cudrania tricuspidata. Figure 3 is a figure showing the experimental schedule for inducing immunodeficiency and treating extract samples. Figure 4 is a photograph showing the spleen of a mouse excised after administration of red ginseng extract and / or Cudrania tricuspidata extract samples for 10 days following immunosuppression induction. Figure 5 is a graph showing the concentrations of IgG (A) and IgM (B) in the serum of mouse blood collected after administration of red ginseng extract and / or Cudrania tricuspidata extract samples for 10 days following immunodeficiency induction. Specific details for implementing the invention

[0034] The present invention will be explained in more detail below through one or more embodiments. However, these embodiments are intended to illustrate the invention and the scope of the invention is not limited to these embodiments.

[0036] Example 1. Preparation of Red Ginseng Extract Sample

[0037] 1-1. Preparation of Red Ginseng Concentrate

[0038] 6-year-old domestic red ginseng in good condition, free from foreign matter and off-odor, was purchased, and red ginseng roots and red ginseng rhizomes were prepared in a weight ratio of 6:4. Then, the mixture was heated 4 to 6 times using water, alcohol, or a mixture thereof to extract the product. After cooling the extract, the precipitate was removed by centrifugation. Next, the filtered liquid was aged and concentrated by reducing the amount of carbon dioxide to adjust the solid content to 60.1%, thereby producing a red ginseng concentrate having a unique flavor and taste, and a blackish-brown viscous liquid form free from off-odor and off-odor.

[0039] The total content of ginsenosides Rg1, Rb1, and Rg3 contained in the manufactured red ginseng concentrate was confirmed to be 13.22 mg / g, and the total saponin was confirmed to be 72.8 mg / g, and no bacteria, coliforms, or microorganisms (mold and yeast) were detected.

[0041] 1-2. Preparation of freeze-dried red ginseng concentrate powder

[0042] 35.1 g (68.6 brix) of red ginseng concentrate prepared in Example 1-1 was mixed with 152.2 g of heated distilled water to dilute to 13 brix, filtered through a 25 µm filter, and pre-frozen at -80°C (Fig. 1). Then, 22.3 g of freeze-dried red ginseng concentrate powder was obtained by freeze-drying at -52°C, and the freeze-drying yield was confirmed to be 11.9%.

[0044] Example 2. Preparation of Cudrania tricuspidata extract sample

[0045] Commercially available dried Cudrania tricuspidata fruit (Saehan Mulsan) was washed and ground. Then, 300g of the ground dried Cudrania tricuspidata fruit was mixed with 2700g of water and heated at 105℃ for 15.5 hours to prepare a hot water extract of dried Cudrania tricuspidata. Afterward, the hot water extract was filtered through a 25㎛ filter, concentrated to 20 Brix at 80℃, and then pre-frozen at -60℃. Subsequently, it was freeze-dried at -50℃ to obtain 87.7g of freeze-dried powder of the hot water extract of dried Cudrania tricuspidata (Fig. 2), and the freeze-drying yield was confirmed to be 10.6%.

[0047] Example 3. Method for administering red ginseng and / or Cudrania tricuspidata samples to an animal model of immunodeficiency induced by cyclophosphamide.

[0048] 3-1. Preparation of a Cyclophosphamide-Induced Immunodeficiency Animal Model

[0049] After acclimatizing C57BL / 6 mice to the experimental environment for one week, cyclophosphamide was administered intraperitoneally at a dose of 150 mg / kg, followed by intraperitoneal administration of cyclophosphamide at a dose of 110 mg / kg three days later, and an immunosuppressed animal model was prepared one day later. The experimental schedule for immunosuppression induction and extract sample treatment is shown in Figure 3. The mice were allowed free access to water and feed during the experiment.

[0051] 3-2. Administration of Red Ginseng and / or Cudrania Trichosanthes Samples to Immunodeficiency Animal Models

[0052] The animal model in which immunosuppression was induced in Example 3-1 was classified into a normal group, a negative control group, and an experimental group, and the red ginseng extract sample prepared in Example 1 and / or the Cudrania tricuspidata extract sample prepared in Example 2 were administered daily as shown in Table 1 for 10 days after immunosuppression was induced. The Cudrania tricuspidata extract and red ginseng extract complex sample was used by mixing the extracts in the respective weight ratios.

[0054] group Administered Sample and Dosage Normal group doesn't exist Negative control group (Veh) Distilled water (200 mg / kg, orally administered) experimental group Cudrania Cudrania tricuspidata (200mg / kg, orally administered) Red ginseng Red ginseng (200mg / kg, orally administered) Cudrania tricuspidata + Red Ginseng 8:2 Cudrania tricuspidata + Red Ginseng (8:2) (200mg / kg, orally administered) Cudrania tricuspidata + Red Ginseng 5:5 Cudrania tricuspidata + Red Ginseng (5:5) (200mg / kg, orally administered) Cudrania tricuspidata + Red Ginseng 2:8 Cudrania tricuspidata + Red Ginseng (2:8) (200mg / kg, orally administered)

[0056] Example 3. Confirmation of changes in body weight, food intake, and water intake following the administration of red ginseng extract and / or Cudrania tricuspidata extract samples

[0057] For an immunocompromised animal model administered red ginseng extract and / or Cudrania tricuspidata extract samples according to the administration method of Example 3, changes in body weight, food intake, and water intake were measured compared to before immunocompromise induction.

[0058] Specifically, the body weight, daily intake, and daily water intake of mice in each group were measured the day before immunosuppression was induced with cyclophosphamide, and the body weight, daily intake, and daily water intake of mice in each group were measured after the administration of red ginseng extract and / or Cudrania tricuspidata extract samples was completed for 10 days after immunosuppression was induced, and the changes were evaluated as shown in Table 2.

[0060] division weight intake (g / day) Water intake (ml / day) First (g) Final (g) Change (g / 14 days) Normal group 24.35±0.24 24.44±0.24 0.08±0.19*** 27.09±0.59* 19.94±1.45* Negative control group 25.01±0.49 23.94±0.59 -1.07±0.18 18.20±0.26 12.02±1.84 Cudrania 24.68±0.46 22.73±0.84 -1.45±0.14 22.46±1.70 12.75±0.26 Red ginseng 25.13±0.27 22.84±0.63 -1.77±0.20* 22.18±1.39 8.21±0.95 Cudrania tricuspidata + Red Ginseng 8:2 24.55±0.40 22.15±0.89 -1.88±0.38 28.24±2.49* 13.63±2.09 Cudrania tricuspidata + Red Ginseng 5:5 24.77±0.40 22.21±0.51* -2.55±0.51* 20.25±2.95 10.33±3.24 Cudrania tricuspidata + Red Ginseng 2:8 24.87±0.54 22.67±0.58 -2.20±0.30** 17.56±0.38 12.61±0.69 Data Display: Mean ± Standard Deviation Significance: * p <0.05, ** p <0.01, *** p <0.001 vs . Negative control group

[0062] As a result, significant weight loss was observed in the groups administered red ginseng extract alone, and Cudrania tricuspidata extract and red ginseng extract in a weight ratio of 5:5 or 2:8 compared to the negative control group with induced immunosuppression. On the other hand, no significant weight loss was observed in the group administered Cudrania tricuspidata extract and red ginseng extract in a weight ratio of 8:2.

[0063] Through these results, it was confirmed that body weight can be reduced by red ginseng extract in immunocompromised mice, and that co-administration of Cudrania tricuspidata extract is effective to suppress such reduction, with a weight ratio of Cudrania tricuspidata extract to red ginseng extract being 8:2.

[0065] Example 4. Confirmation of the recovery effect of spleen tissue following administration of red ginseng extract and / or Cudrania tricuspidata extract samples

[0066] For an immunocompromised animal model administered red ginseng extract and / or Cudrania tricuspidata extract samples according to the administration method of Example 3, changes in spleen tissue were measured compared to before immunocompromise induction.

[0067] Specifically, after administering red ginseng extract and / or Cudrania tricuspidata extract samples for 10 days following immunosuppression induction, the mice were euthanized after blood collection, and the spleen was removed and its weight measured (Table 3). To eliminate variation due to differences in the experimental animals' body weights and to standardize the spleen tissue weight, it was expressed as the relative tissue weight (% of body weight) relative to body weight.

[0068] In addition, to more specifically confirm the significant synergistic effect of the complex extract administration group, the spleen tissue weight of each group was converted into a percentage based on 100% of the normal group's spleen weight, and the percentage predicted value of the mixed extract was derived by substituting the actual percentage of the single extract administration group into the Colby formula below.

[0070] E = (A + B) - (A× / 100)

[0072] In the above formula, A is the medicinal effect of the active ingredient Cudrania tricuspidata, B is the medicinal effect of the active ingredient red ginseng, and E is a predicted value representing the predicted medicinal effect when Cudrania tricuspidata and red ginseng are mixed.

[0074] division Spleen weight (g / 100g body weight) Actual percentage relative to normal group (%) Predicted percentage compared to normal group (%) Normal group 0.318 ± 0.008*** 100 - Negative control group 0.142 ± 0.001 44.7 - Cudrania 0.149 ± 0.002* 46.7 - Red ginseng 0.147 ± 0.004* 46.2 71.3 Cudrania tricuspidata + Red Ginseng 8:2 0.196 ± 0.009*** 61.6 Cudrania tricuspidata + Red Ginseng 5:5 0.157 ± 0.014 49.4 Cudrania tricuspidata + Red Ginseng 2:8 0.154 ± 0.020 48.4 Data Display: Mean ± Standard Deviation Significance: * p <0.05, ** p <0.01, *** p <0.001 vs . Negative control group

[0076] As a result, it was confirmed that the reduced spleen weight was not significantly recovered in the groups administered Cudrania tricuspidata extract and red ginseng extract in a weight ratio of 5:5 or 2:8. On the other hand, it was confirmed that the recovery effect of spleen weight was excellent in the group administered Cudrania tricuspidata extract and red ginseng extract in a weight ratio of 8:2 (Fig. 4), which was found to be a superior effect compared to the administration of Cudrania tricuspidata extract alone and red ginseng extract alone.

[0077] Through these results, it was confirmed that the reduced spleen weight in immunocompromised mice can be effectively restored by the combined use of Cudrania tricuspidata extract and red ginseng extract, and that a synergistic effect on spleen weight recovery was observed at an 8:2 weight ratio of Cudrania tricuspidata extract to red ginseng extract.

[0079] Example 5. Confirmation of the effect of administration of red ginseng extract and / or Cudrania tricuspidata extract samples in increasing serum immunoglobulin concentration

[0080] The serum immunoglobulin concentration of an immunodeficient animal model administered red ginseng extract and / or Cudrania tricuspidata extract samples according to the administration method of Example 3 was measured, and the change in immunoglobulin concentration compared to the normal group was evaluated.

[0081] Specifically, the blood collected in Example 4 was placed in a centrifuge tube and centrifuged at 2,000 rpm and 4°C for 15 minutes to obtain serum. Subsequently, the concentrations of IgG (Table 4) and IgM (Table 5) in the serum were measured according to the protocol of BETHYL, the manufacturer of the ELISA kit.

[0082] In addition, to more specifically confirm the significant synergistic effect of the complex extract administration group, the immunoglobulin concentrations of each group were converted into percentages based on the serum concentration of the normal group as 100%, and the predicted percentage of the mixed extract was derived by substituting the actual percentage of the single extract administration group into the Colby formula below.

[0084] E = (A + B) - (A× / 100)

[0086] In the above formula, A is the medicinal effect of the active ingredient Cudrania tricuspidata, B is the medicinal effect of the active ingredient red ginseng, and E is a predicted value representing the predicted medicinal effect when Cudrania tricuspidata and red ginseng are mixed.

[0088] division IgG(ng / ml) Actual percentage relative to normal group (%) Predicted percentage compared to normal group (%) Normal group 144.59±3.49*** 100 - Negative control group 112.60±1.95 77.9 - Cudrania 124.37±3.40* 86.0 - Red ginseng 123.49±2.41* 85.4 98.0 Cudrania tricuspidata + Red Ginseng 8:2 132.01±3.32** 91.3 Cudrania tricuspidata + Red Ginseng 5:5 127.31±3.02* 88.0 Cudrania tricuspidata + Red Ginseng 2:8 126.57±4.64* 87.5 Data Display: Mean ± Standard Deviation Significance: * p <0.05, ** p <0.01, *** p <0.001 vs . Negative control group

[0090] division IgM(ng / ml) Actual percentage relative to normal group (%) Predicted percentage compared to normal group (%) Normal group 19.25±1.72*** 100 - Negative control group 14.33±0.22 74.4 - Cudrania 15.93±0.28* 82.8 - Red ginseng 15.90±0.35** 82.6 97.0 Cudrania tricuspidata + Red Ginseng 8:2 18.91±0.68*** 98.2 Cudrania tricuspidata + Red Ginseng 5:5 16.80±16.61** 87.3 Cudrania tricuspidata + Red Ginseng 2:8 16.60±0.45** 86.2 Data Display: Mean ± Standard Deviation Significance: * p <0.05, ** p <0.01, *** p <0.001 vs . Negative control group

[0092] As a result, it was confirmed that the concentrations of immunoglobulins IgG and IgM in the serum of mice decreased due to the induction of immunosuppression. Meanwhile, among all administration groups, the group administered Cudrania tricuspidata extract and red ginseng extract in a weight ratio of 8:2 showed the most superior increase in serum IgG and IgM (Fig. 5). In particular, when Cudrania tricuspidata and red ginseng were administered in a mixed ratio of 8:2, a significant synergistic effect was observed with respect to serum IgM concentration, with the actual value (98.2%) exceeding the predicted value (97.0%).

[0093] Through these results, it was confirmed that the reduced serum immunoglobulin concentration in immunocompromised mice can be effectively increased by the combined use of Cudrania tricuspidata extract and red ginseng extract, and that the synergistic effect on the increase of serum IgG and IgM was most excellent at an 8:2 weight ratio of Cudrania tricuspidata extract to red ginseng extract, and that the synergistic effect on IgM among immunoglobulins was particularly excellent.

[0095] The present invention has been described above with reference to its embodiments. Those skilled in the art will understand that the present invention may be embodied in modified forms without departing from the essential characteristics of the invention. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the invention is defined by the claims, not by the foregoing description, and all variations within the scope of equivalents should be interpreted as being included in the invention.

Claims

Claim 1 A health functional food for immune enhancement, wherein the health functional food comprises a Cudrania tricuspidata extract and a red ginseng extract, wherein the Cudrania tricuspidata is a Cudrania tricuspidata fruit, the extraction solvent of each extract is water, and the weight ratio of the Cudrania tricuspidata extract and the red ginseng extract is 7:3 to 9:

1. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 A health functional food for immune enhancement according to claim 1, wherein the immune enhancement is due to an increase in the concentration of IgM in the serum. Claim 6 A pharmaceutical composition for immune enhancement, wherein the composition comprises a Cudrania tricuspidata extract and a red ginseng extract, wherein the Cudrania tricuspidata is a Cudrania tricuspidata fruit, the extraction solvent of each extract is water, and the weight ratio of the Cudrania tricuspidata extract and the red ginseng extract is 7:3 to 9:1.

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