Protein-supplied composition containing sprout wheat extract that may help bone, muscle and joint health

Abstract

The present invention relates to a protein supply composition containing sprouted wheat extract that can help with bone, muscle, and joint health, characterized by comprising 34.1% by weight of isolated soy protein, 27.3% by weight of concentrated whey protein, 25.6% by weight of concentrated milk protein, 8.5% by weight of Dirac total milk powder, and 4.5% by weight of sprouted wheat extract. The present invention has the effect of comprising an optimal nutritional composition developed to substantially prevent muscle loss by containing high-quality plant-based and animal-based proteins, along with sprout wheat extract, a functional ingredient that provides protein necessary for muscle growth, as well as functions for preventing osteoporosis, preventing muscle loss, and anti-inflammatory effects.

Description

Technology Field

[0001] The present invention relates to a protein supply composition containing wheat sprout extract that can help with bone, muscle, and joint health. More specifically, the invention relates to a protein supply composition containing wheat sprout extract that can help with bone, muscle, and joint health, comprising an optimal nutritional composition developed to substantially prevent muscle loss by including high-quality plant protein and animal protein along with wheat sprout extract, which is a functional ingredient possessing anti-inflammatory functions and also provides protein necessary for muscle growth. Background Technology

[0002] Generally, the three major factors that impede healthy life expectancy due to aging are metabolic syndrome, motor decline syndrome, and cognitive disease, all of which have organic correlations.

[0003] The above-mentioned locomotive syndrome refers to a condition in which the motor organs necessary for movement, such as bones, joints, muscles, tendons, ligaments, and nerves, weaken, making it difficult to perform basic movements like standing or walking, and requiring care, or a condition with a high risk thereof.

[0004] If metabolic syndrome is a lifestyle disease, physical movement deteriorates due to various complications and becomes a cause of motor dysfunction syndrome; conversely, if motor dysfunction syndrome is present, exercise cannot be considered, which can lead to the onset or further worsening of lifestyle diseases. Furthermore, motor dysfunction syndrome and metabolic syndrome increase the risk of developing cognitive disease, and cognitive disease can further exacerbate motor dysfunction or metabolic syndrome.

[0005] Compared to the incidence and importance of age-related decline in motor function syndrome, the system for preventing and managing it is inadequate in Korea. In Japan, which entered an aging society earlier than Korea, the 'New Health Japan 21' initiative highlighted the need to maintain and improve the health of joints, muscles, and bones as a countermeasure against decline in motor function syndrome.

[0006] Prevention and care are crucial for motor function decline syndrome. To control it, exercise and nutrition must be properly balanced. This requires checking several diagnostic criteria related to movement to implement dietary management and exercise therapy necessary for strengthening bones, joints, and muscles, as well as weight control, while also ensuring the appropriate intake of health functional foods containing various functional ingredients.

[0007] The functional materials for bones, joints, and muscles currently available in the market are very limited, and functional food materials that provide overall efficacy for these three elements have not been developed. There is a need to develop materials that are economically viable and have few side effects.

[0008] For the effective prevention and improvement of motor decline syndrome, it is necessary to combine the development of functional foods with systematic exercise therapy, and to simultaneously develop various services to enable elderly consumers to continuously maintain and manage improvement programs.

[0009] In this regard, the domestic market for products related to motor function decline syndrome is very underdeveloped, and products targeting the health of specific areas such as bones, joints, and muscles are available, while the relevant materials are very limited, such as calcium, vitamins, proteins, and glucosamine.

[0010] In this regard, "a pharmaceutical composition for the prevention or treatment of osteoporosis comprising a wheat sprout extract, wherein the wheat sprout extract is obtained by extracting wheat sprouts with one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, and mixed solvents thereof, wherein the wheat sprout extract inhibits the differentiation of osteoclasts and further comprises a pharmaceutically acceptable carrier, excipient, or diluent" was disclosed in Patent Registration No. 10-2081984 (published on February 26, 2020).

[0011] The term "sprouted wheat" above refers to a young plant body that has sprouted wheat and grown to about 15 cm. Typically, the sprouted wheat can be obtained by soaking wheat in water for about a day, removing the moisture, sowing it under conditions that prevent moisture loss to germinate it, and then growing it for 3 to 20 days.

[0012] In this prior art, it was confirmed that wheat sprout extract inhibits osteoclast differentiation induced by RANKL, and that this inhibitory effect is not caused by cytotoxicity, and it can be seen that wheat sprout extract can be used to prevent, treat, or improve osteoporosis.

[0013] In addition, regarding a composition using sprout wheat extract, "a food composition for improving sarcopenia comprising a wheat sprout ethanol extract as an active ingredient, wherein the sarcopenia is selected from the group consisting of myofascial rigidity, hypotonia, muscle weakness, and myasthenia gravis, and the wheat sprout ethanol extract inhibits the reduction of root canal size, inhibits muscle cell apoptosis, promotes muscle differentiation, or inhibits muscle protein degradation, and wherein the sarcopenia is caused by aging or obesity" was disclosed in Patent Registration No. 10-2487651 (published on January 12, 2023).

[0014] However, the aforementioned prior art involves ingredients individually certified as functional ingredients for health supplements that aid in the health of bones, joints, and muscles. Although there has recently been a trend of products being released that combine these ingredients, no functional ingredient has been developed to date that helps with the health of bones, joints, and muscles all at once, and single food ingredients with combined functions related to exercise and health are rarely provided in the global market. Prior art literature

[0015] (KR) Republic of Korea Patent No. 10-2081984 (Published Feb. 26, 2020) (KR) Republic of Korea Patent No. 10-2487651 (Published Jan. 12, 2023) The problem to be solved

[0016] The present invention aims to solve the aforementioned problems by providing a protein supply composition containing sprout wheat extract that can practically help with bone, muscle, and joint health. This composition is composed of an optimal nutritional composition developed to substantially prevent muscle loss by including high-quality plant and animal proteins, along with sprout wheat extract, a functional ingredient that provides protein necessary for muscle growth and possesses anti-inflammatory functions such as preventing osteoporosis and muscle loss.

[0017] The problems that the present invention aims to solve are not limited to those mentioned above, and other unmentioned problems will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0018] To achieve this objective, the present invention is characterized in that the protein supply composition containing wheat sprout extract comprises 3.0 to 10.0 weight% of wheat sprout extract and 90 to 97 weight% of animal and plant proteins.

[0019] The above is characterized by having 55 to 65 weight% of animal protein and 35 to 45 weight% of plant protein.

[0020] The above plant protein is isolated soy protein; and the above animal protein is characterized by being one or more selected from concentrated whey protein, concentrated milk protein, and Dirac total milk powder.

[0021] A protein supply composition containing wheat sprout extract is characterized by comprising 34.1% by weight of isolated soy protein, 27.3% by weight of concentrated whey protein, 25.6% by weight of concentrated milk protein, 8.5% by weight of Dilac total milk powder, and 4.5% by weight of wheat sprout extract. Effects of the invention

[0022] Thus, the present invention comprises an optimal nutritional composition developed to substantially prevent muscle loss by containing high-quality plant and animal proteins, along with sprout wheat extract, a functional ingredient that provides protein necessary for muscle growth, as well as functions for preventing osteoporosis, preventing muscle loss, and anti-inflammatory effects. The composition has the effect of enabling the manufacture of various foods in the form of beverages and powders.

[0023] The present invention is not limited to the specific preferred embodiments described above, and anyone with ordinary knowledge in the art to which the invention pertains can make various modifications without departing from the essence of the invention as claimed in the claims, and such modifications will be within the scope of the claims. Brief explanation of the drawing

[0024] Figure 1 is a comparison graph with a control group showing the effect of a protein supply composition containing sprout wheat extract, which is an embodiment of the present invention and can help with bone, muscle, and joint health, on inhibiting the reduction of root canal size. Figure 2 is a comparison graph with a control group showing the effect of a protein supply composition containing sprout wheat extract, which is an embodiment of the present invention and can help with bone, muscle, and joint health, on inhibiting the production of the inflammatory mediator TNF-α. Specific details for implementing the invention

[0025] The present invention will be described in more detail below with reference to the attached drawings.

[0026] Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings so that those skilled in the art can easily implement them. The present invention may be embodied in various different forms and is not limited to the embodiments described herein.

[0027] In order to clearly explain the invention in the drawings, parts unrelated to the explanation have been omitted, and the same reference numerals have been used for identical or similar components throughout the specification. The scope of the invention should not be interpreted as being limited by the embodiments described in the text, and since the embodiments are subject to various modifications and may take various forms, the scope of the invention should be understood to include equivalents capable of realizing the technical concept.

[0028] Throughout the specification of the present invention, when a part is described as being "connected" to another part, this includes not only cases where they are "directly connected," but also cases where they are "indirectly connected" with other members in between. Furthermore, when a part is described as "including" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0029] The terms used in this invention are defined in consideration of their functions within the invention; however, since these may vary depending on the intentions or practices of the user or operator, the definitions of these terms should be interpreted in a sense and concept consistent with the technical details of this invention.

[0030] Furthermore, optional terms in the embodiments below are used to distinguish one component from another, and the components are not limited by said terms. In describing the present invention below, detailed descriptions of related prior art that may unnecessarily obscure the essence of the invention are omitted.

[0031] Muscle is one of the indicators of health in old age; it supports the body, provides necessary exercise capabilities, and serves as a storehouse for energy required for activity. However, as one ages, degenerative changes lead to a significant decline in physical function and muscle loss, which can have a negative impact on overall health.

[0032] Consequently, as motor skills and balance decline, the risk of safety accidents such as falls increases, and the likelihood of developing chronic diseases also rises; therefore, in old age when sarcopenia occurs, one must pay more attention to muscle health than in youth.

[0033] To prevent sarcopenia in old age, you must pay more attention to "first, doing muscle exercises; second, drinking 1 liter of water a day; and third, consuming adequate nutrition such as protein."

[0034] Consuming high-quality protein, which serves as the building block for muscles, helps build muscle strength and prevent sarcopenia. The recommended daily protein intake for a typical adult is about 0.8g per kg of body weight, while for the elderly, it is recommended to consume 1 to 1.5g of protein per kg of body weight to prevent and treat sarcopenia.

[0035] At this time, clinical trial results have reported that consuming a mixture of plant-based and animal-based proteins can extend the duration of anabolic action for increased muscle mass and muscle strengthening. In other words, when plant-based and animal-based proteins are mixed, one can utilize the advantages of each protein source, such as specific amino acid content, amino acid delivery time, and other health benefits. For example, whey protein has a high leucine content and delivers amino acids rapidly, while soy protein not only has high levels of arginine and glutamine but also naturally produces antioxidants that aid in muscle recovery.

[0036] The protein supply composition of the present invention, which contains a sprouted wheat extract that can help with bone, muscle, and joint health, consists of 3.0 to 10.0 weight% of sprouted wheat extract and 90 to 97 weight% of animal and plant proteins.

[0037] If the content of wheat sprout extract in the above composition is less than 3% by weight, the efficacy of inhibiting muscle loss and preventing osteoporosis is reduced, so it is not an effective composition, and if the content of wheat sprout extract exceeds 10% by weight, the cost increases rapidly, and commercial practicality decreases.

[0038] It is preferable that the above animal protein is 55 to 65 weight% and the plant protein is 35 to 45 weight%.

[0039] The above plant protein is isolated soy protein, and the above animal protein is preferably one or more selected from concentrated whey protein, concentrated milk protein, and Dirac total milk powder.

[0040] Clinical trial results have reported that consuming a mixture of plant-based and animal proteins can extend the duration of anabolic action for increased muscle mass and muscle strengthening (Source: Paul et al. J AM Coll Nutr 2009).

[0041] In other words, when plant and animal proteins are mixed, the advantages of each protein source, such as specific amino acid content, amino acid delivery time, and other health benefits, can be utilized. For example, whey protein has a high leucine content and delivers amino acids rapidly, while soy protein is not only high in arginine and glutamine but also naturally produces antioxidants that help muscle recovery.

[0043] The protein supply composition containing wheat sprout extract that can help with bone, muscle, and joint health according to Example 1 of the present invention consists of 34.1% by weight of isolated soy protein, 27.3% by weight of concentrated whey protein, 25.6% by weight of concentrated milk protein, 8.5% by weight of Dirac total milk powder, and 4.5% by weight of wheat sprout extract.

[0044] When the protein components in the above composition are classified into plant protein and animal protein and their content is calculated, the ratio of animal protein to plant protein is 61:39, as shown in (Table 1) below.

[0046] Protein component of the composition of the present invention division type Composition ratio (%) Protein content (%) Protein amount (g) ratio(%) plant-based protein Soy protein isolate 34.1 88 30.01 39 animal protein Whey protein concentrate 27.3 80 21.84 28 milk protein concentrate 25.6 85 21.76 28 Dirac Total Milk Powder 8.5 45 3.83 5 total 95.5 77.43 100

[0048] The results of the nutritional analysis of the above composition are as follows (Table 2).

[0049] When food is manufactured using the composition of the present invention, if used to allow for a daily intake of 73g, the recommended daily protein intake of 55g is met, and the daily protein intake standard of 12g according to the Health Functional Food Codex is achieved with 16g of the present ingredient.

[0051] Nutritional components of the composition of the present invention ingredient Per 100g Percentage (%) of daily nutritional value note Calories 370kcal - sodium 440mg 22 carbohydrate 13g 4 Sugars 3g 3 province 1.8g 3 trans fat 0g - saturated fat 0.9g 6 unsaturated fat 0g - cholesterol 85mg 28 protein 75g 136

[0052] (However, the percentage of the daily nutritional value is based on a 2,000kcal diet.)

[0054] The Ministry of Food and Drug Safety evaluates protein quality using the 'amino acid score,' which is a protein quality report card that compares the quality scores of the nine essential amino acids contained in the protein, and the Health Functional Food Codex recognizes that a protein can function only if this score exceeds 85 points.

[0055] The amino acid score is defined as the ratio of the lowest amino acid among the percentages calculated by analyzing the types and content of amino acids in the protein contained in the product, matching the units with the amino acid content of the standard essential amino acid composition table, and converting them into percentages.

[0056] The results of analyzing the amino acid score of the composition of the present invention showed a high amino acid score of 135.27, as shown in (Table 3), indicating that it is a protein of excellent quality.

[0058] Amino acid score of the composition of the present invention Histidine isoleucine Leucine Lysine Methionine + Cystine Phenylalanine + Tyrosine Threonine tryptophan Valin Reference value 15 30 59 45 22 38 23 6 39 Measured value 21.31 49.13 82.75 68.08 27.37 73.98 42.36 14.39 52.76 %value 142.07 163.78 140.26 151.29 124.41 194.69 184.18 239.84 135.27

[0059] (Note: Unit: mg / g crude protein)

[0062] The osteoblast formation-promoting effect of the composition of the present invention

[0064] In order to investigate its utility value as a substance for the prevention and treatment of osteoporosis, it is necessary to investigate its functionality as a bone formation promoter rather than a bone resorbent.

[0065] Accordingly, the effect of the composition of the present invention on the promotion of osteoblast formation, which plays a key role in promoting bone formation, was investigated.

[0066] The differentiation medium for osteoblasts isolated from mice was prepared by adding ascorbic acid (0.1 mg / ml) and β-glycerol phosphate (5 mM), which are osteoblast differentiation and activating factors, to α-MEM containing 10% FBS and mixing them. Using the above differentiation medium, 2.5 × 10 -3 1×10 of the sample of the composition of the present invention in g / ml -5 and 1×10-7 The sample was diluted to a concentration of g / ml.

[0067] To prepare the AR-S staining solution (40 mM Alizarin), 136.8 mg of Sigma's Alizarin Red S was dissolved in 10 ml of water and filtered using a 0.25 µm filter. For Vonkossa (5% silver nitrate) staining, 5 g of silver nitrate was dissolved in ultrapure water to a final volume of 100 ml to prepare a 5% (w / v) silver nitrate staining solution. The cetylpyrinidinium chloride solution required for the AR-S elution experiment was prepared by dissolving the solution in a 10 mM sodium phosphate buffer solution (pH 7.0) at a concentration of 10% (w / v). The AR-S standard solution was prepared by mixing 73 µl of 40 mM AR-S with 927 µl of 10% cetylpyrinidinium chloride solution to a concentration of 1 mg / ml, and then diluting it to various concentrations for use.

[0068] The prepared samples were added to the plates of the above-mentioned medium every 3 days after the medium was completely removed. After 15 days of incubation, the medium was completely removed and the samples were washed several times with purified water. Then, 1 ml of 10% formalin aqueous solution was added to each well and fixed for 30 minutes. After fixation, the samples were washed once with water, then 500 µl of the Alizarin Red S (40mM Alizarin Red S) staining solution or Vonkossa' (5% silver nitrate) staining solution prepared above was added and reacted for 30 minutes, after which they were washed several times with water and dried.

[0069] Through the above staining, the accumulated amount of phosphorus or calcium could be calculated by area, or in the case of AR-S, by extracting the staining solution and measuring the absorbance. The area was measured by using an image analyzer (Laica Co.) to determine the stained area and expressing it as a percentage (%) relative to the control group. Additionally, for the AR-S staining method, the calculation of the accumulated amount of calcium was as described above, and it could also be measured by absorbance obtained through the elution of AR-S from the staining solution. To elute AR-S from the stained plate, 500 µl of 10% cetylpyrinidinium chloride solution was added and reacted at 37°C for 2 hours. The absorbance of the eluted AR-S solution was measured at 560 nm, and the exact amount of eluted AR-S was calculated using a standard graph prepared by measuring the absorbance at different concentrations of the AR-S solution.

[0071] Osteoblast formation-promoting effect of the composition of the present invention containing wheat sprout extract and protein Sample 1×10 -5 g / ㎖ 1×10 -7 g / ㎖ AR-S amount (µg / ml) Osteoblast activity (%) AR-S amount (µg / ml) Osteoblast activity (%) Composition of the present invention 336±3 118 394±38 185 control group 290±25.6 100 215±64.1 100

[0073] As shown in (Table 4), it can be seen that the composition of the present invention has a higher effect in promoting osteoblast formation compared to the control group.

[0074] This result demonstrates that wheat sprout extract and protein components are also effective in bone formation through the accumulation of calcium in osteoblasts.

[0077] The efficacy of the composition of the present invention in improving sarcopenia

[0079] To confirm the inhibitory effect of wheat sprout extract on sarcopenia in the aforementioned root cells through a muscle atrophy induction experiment using a cell culture model, the inhibitory effect on the reduction of root canal size at different concentrations of wheat sprout extract was analyzed.

[0080] To construct an in vitro sarcopenia cell culture model, myoblasts were isolated and cultured from the skeletal muscle of 3-day-old C57BL / 6 mice. Skeletal muscle, with the skeleton and fat removed, was washed in PBS, and cells were isolated by treating with a 1:1 mixture of 1.5 U / mL Collagenase D and 2.4 U / mL dispase for 1 hour. The isolated cells were settled to form a cell pellet, which was then dispensed into a culture medium. After removing fibroblasts, the cells were cultured in a medium. The experiment was conducted using collagen-coated medium to induce muscle differentiation. The culture medium used was Ham's F-10 medium supplemented with 10% calf serum, 1% antibiotics (100 U / ml penicillin, 100 μg / ml streptomycin), and 2.5 μg / ml fibroblast growth factor, while for differentiation, Dulbecco's modified Eagle medium supplemented with 1% horse serum and 1% antibiotics (100 U / ml penicillin, 100 μg / ml streptomycin). Cultures were performed under conditions of 37℃ and 5% CO2 in a culture chamber.

[0081] Root cells cultured under the above conditions were treated with the composition of the present invention containing wheat sprout extract at concentrations of 10, 50, and 100 μg / mL and cultured for 24 to 48 hours, and the size of the root canal was measured using a dimeter measuring device.

[0082] As a result, as shown in Figure 1, it was confirmed that the composition of the present invention significantly inhibits the reduction of root canal size in a concentration-dependent manner.

[0085] Anti-inflammatory effect of the composition of the present invention

[0087] 1.5 × 10⁶ RAW 264.7 cells in a 96-well plate 5Cells were inoculated into wells and cultured for 12 hours, then treated with LPS (10 μg / mL) and the composition of the present invention at concentrations of 10, 50, 100, and 500 μg / mL simultaneously, and cultured for 24 hours to obtain the cell culture medium, after which the TNF-α contained in the culture medium was measured using an ELISA kit.

[0088] As a result of examining the effect of the composition of the present invention on TNF-α secretion, as shown in Figure 2, it was observed that TNF-α production was inhibited in a concentration-dependent manner.

[0090] The embodiments and drawings attached to this specification merely clearly illustrate a part of the technical concept included in the present invention, and it is obvious that variations and specific embodiments that can be easily deduced by a person skilled in the art within the scope of the technical concept included in the specification and drawings of the present invention are all included within the scope of the technical concept of the present invention.

[0091] The scope of the present invention is defined by the claims set forth below rather than by the detailed description, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts should be interpreted as being included within the scope of the present invention.

Claims

Claim 1 A protein supply composition containing a sprouted wheat extract that can help with bone, muscle, and joint health, characterized in that it comprises 3.0 to 10.0 weight% of sprouted wheat extract and 90 to 97 weight% of animal and plant proteins, wherein the animal protein is 55 to 65 weight% and the plant protein is 35 to 45 weight%, the plant protein is isolated soy protein, and the animal protein is one or more selected from concentrated whey protein, concentrated milk protein, and Dilac Total Fat Powder. Claim 2 delete Claim 3 delete Claim 4 A protein supply composition containing sprouted wheat extract, animal protein, and plant protein, characterized by comprising 34.1% by weight of isolated soy protein, 27.3% by weight of concentrated whey protein, 25.6% by weight of concentrated milk protein, 8.5% by weight of Dilac total milk powder, and 4.5% by weight of sprouted wheat extract, which can help with bone, muscle, and joint health.

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