Skin care composition comprising fish extract and hyaluronic acid

Abstract

The present disclosure relates to a skin cosmetic composition comprising a fish extract and hyaluronic acid, and more specifically, to a skin cosmetic composition that improves skin elasticity and provides a moisturizing effect by comprising a fish extract derived from salmon and / or trout and low molecular weight hyaluronic acid.

Description

Technology Field

[0001] The present disclosure relates to a skin cosmetic composition comprising a fish extract and hyaluronic acid, and more specifically, to a skin cosmetic composition that improves skin elasticity and provides a moisturizing effect by comprising a fish extract derived from salmon and / or trout and low molecular weight hyaluronic acid. Background Technology

[0002] While fish are commonly consumed as food and contain a balanced variety of nutrients, the various by-products generated during processing are often discarded or converted into livestock feed, resulting in the insufficient utilization of the beneficial components contained within them. In particular, for fish such as salmon and trout, by-products produced during processing into fillets and other forms are mostly discarded despite still containing high nutritional value and various components with special functions, raising concerns about resource waste.

[0003] Furthermore, when fish are consumed as food, individuals that are small or irregular in shape are generally not processed and are discarded along with processing by-products for reasons of marketability. However, even fish discarded for such reasons contain nutritional components equivalent to those of high-quality fish; consequently, the disposal of such individuals results in a waste of resources and is a cause of various forms of environmental pollution.

[0004] Meanwhile, although products based on various basic ingredients are being proposed as cosmetic compositions that improve skin elasticity and provide moisturizing effects, most products on the market contain artificially synthesized ingredients as their main components. Even when eco-friendly ingredients are included, obtaining them requires creating an environment where the source of the ingredients is artificially grown; consequently, from an overall perspective, there is a concern regarding environmental pollution, and the situation is not favorable in terms of carbon footprint. The problem to be solved

[0005] The problem that the present disclosure aims to solve is to provide a skin cosmetic composition comprising a fish extract and hyaluronic acid, which improves skin elasticity and has a moisturizing effect.

[0006] Another problem that the present disclosure aims to solve is to provide a skin care composition utilizing fish extracts derived from fish by-products discarded during processing and fish individuals that are discarded, thereby utilizing the benefits of various useful components contained in fish and providing an environmentally friendly skin care composition.

[0007] The problems that the present disclosure aims to solve are not limited to those mentioned above, and any unmentioned problems that the present disclosure aims to solve will be clearly understood by a person skilled in the art to which the present disclosure belongs ("person skilled in the art") from the description below. means of solving the problem

[0008] According to one embodiment of the present disclosure, a skin cosmetic composition comprising a fish extract and hyaluronic acid may be provided, wherein the fish extract is a fish extract extracted from salmon and / or trout, and the hyaluronic acid is a low molecular weight hyaluronic acid component having a molecular weight of 5 to 50 kDa.

[0009] For example, the fish extract may be included in an amount of 0.1 to 3.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0010] For example, the fish extract may be a component containing PDRN (polydeoxyribonucleotide), and the PDRN may be included in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total fish extract.

[0011] For example, the fish extract may be a component containing PN (polynucleotide), and the PN may be included in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total fish extract.

[0012] For example, the fish extract is a fish extract extracted from salmon, and the fish extract may include an extract extracted from the fins and tail of salmon.

[0013] For example, the fish extract is a fish extract extracted from trout, and the fish extract may include an extract extracted from the fins and tail of a trout.

[0014] For example, the fish extract may consist of a first fish extract extracted from salmon and a second fish extract extracted from trout, wherein the first fish extract may include an extract extracted from the fins and tail of salmon, and the second fish extract may include an extract extracted from the fins and tail of trout.

[0015] For example, the low molecular weight hyaluronic acid may be included in an amount of 15 to 30 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0016] For example, the low molecular weight hyaluronic acid may be a hyaluronic acid component having a molecular weight of 7 to 40 kDA and may have a pH of 6.3 to 6.7.

[0017] For example, the above skin cosmetic composition may additionally include allantoin.

[0018] For example, the allantoin may be included in an amount of 0.1 to 1.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0019] For example, the above skin cosmetic composition may additionally include acetylhexapeptide-8.

[0020] For example, the acetyl hexapeptide-8 may be included in an amount of 0.01 to 0.1 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0021] For example, the above skin beauty composition may additionally include jujube extract.

[0022] For example, the above jujube extract may be included in an amount of 0.5 to 5.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0023] For example, the above skin cosmetic composition may additionally include a herbal mixture of *Clematis terniflora* extract, *Perilla frutescens* extract, *Ligustrum japonicum* extract, and *Ilex crenata* extract.

[0024] For example, the above herbal mixture may be included in an amount of 0.3 to 3.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition. Effects of the invention

[0025] According to the present disclosure, a skin cosmetic composition comprising a fish extract and hyaluronic acid can be provided, which improves skin elasticity and has a moisturizing effect.

[0026] In addition, according to the present disclosure, by providing a skin cosmetic composition utilizing fish extracts extracted from fish by-products discarded during processing and fish individuals that are discarded, it is possible to provide an environmentally friendly skin cosmetic composition that utilizes the benefits of various useful components contained in fish.

[0027] The superior and / or useful effects according to the present disclosure are not limited to the effects of the present disclosure described above, and a person skilled in the art will also be able to obviously recognize, based on the disclosure of the present specification, superior and / or useful effects of the present disclosure that are not explicitly disclosed in the present specification, and it should be understood that these are intentionally disclosed by the present specification and are obviously included within the scope of the present disclosure. Brief explanation of the drawing

[0028] FIG. 1 is a diagram briefly illustrating the composition of a skin cosmetic composition according to one embodiment of the present disclosure. Specific details for implementing the invention

[0029] The terms or words used in this specification and claims are not intended to be interpreted as being limited to their commonly accepted dictionary meanings, and a person skilled in the art will clearly understand that said terms or words are used within the scope of conveying what this disclosure means, within the scope of expressing the concept that this specification and claims obviously intend to convey.

[0030] Furthermore, a person skilled in the art will clearly understand that the configurations described in the aspects, modes of embodiment, examples, etc., of the present disclosure described in this specification are merely preferred examples presented at the time to enable a person skilled in the art to understand and reproduce the present disclosure, and are not intended to limit the present disclosure thereto.

[0031] Furthermore, the descriptions and specific embodiments regarding each configuration described in this specification may be obviously applied to the descriptions and specific embodiments of each other configuration. That is, a person skilled in the art will clearly understand that all possible combinations of the various configurations and specific embodiments disclosed in this specification fall within the scope disclosed herein.

[0032] As used herein, the term "and / or" is a term comprising each of the items mentioned and all combinations of two or more. Additionally, where a singular term is used herein, it is disclosed including the plural form unless otherwise noted.

[0033] As used herein, the terms "comprise" and "comprising" are terms that permit the existence or addition of items other than those mentioned, and as used herein, the terms "consist," "consist," "consisting," and "consisting" are terms that do not permit the existence or addition of items other than those mentioned.

[0034] As used in this specification, the term "to" indicates a numerical range in which the range of values ​​represented by "to" includes the values ​​listed before and after the term as lower and upper values, respectively. Where multiple upper and / or lower values ​​of an arbitrary numerical range are disclosed, the numerical range is disclosed such that any one of the multiple lower values ​​and any one of the multiple upper values ​​are respectively set as the lower and upper values.

[0035] The terms “about” and / or “approximately” as used in this specification refer to a numerical range between a 10% upper limit value and a lower limit value based on the value indicated using “about” and / or “approximately”.

[0036] Terms or words used in this specification and claims should not be interpreted as being limited to their ordinary dictionary meanings, but should be interpreted in a meaning and concept consistent with the technical spirit of this disclosure, based on the principle that the inventor can appropriately define the concept of the terms to best describe his invention. Accordingly, the configurations described in the embodiments of this specification are merely preferred embodiments of this disclosure and do not represent all of the technical spirit of this disclosure; therefore, it should be understood that various equivalents and modifications that can replace them may exist at the time of filing this application.

[0037] Meanwhile, each description and embodiment disclosed in this specification may also be applied to other descriptions and embodiments. That is, all combinations of the various elements disclosed in this specification fall within the scope of this disclosure, and descriptions omitted in one embodiment may be interpreted in the same manner as described in other embodiments. Furthermore, the scope of this disclosure should not be considered limited by the specific descriptions provided below.

[0038] According to one embodiment of the present disclosure, a skin cosmetic composition comprising a fish extract and hyaluronic acid may be provided, wherein the fish extract is a fish extract extracted from salmon and / or trout, and the hyaluronic acid is a low molecular weight hyaluronic acid component having a molecular weight of 5 to 50 kDa.

[0039] General cosmetic compositions mainly contain hyaluronic acid, collagen, ceramide, glycerin, etc., and while they serve to supply moisture and nutrients to the skin and increase skin elasticity and radiance, they have the problem of acting only on the surface of the skin and lacking the ability to penetrate to the deep layers of the skin. The cosmetic composition according to the present disclosure includes fish extract, and based on the tissue similarity and tissue affinity of the components contained in the fish extract, it has excellent ability to be delivered to the deep layers of the skin when applied to the skin, thereby providing excellent effects such as improving skin elasticity and reducing wrinkles.

[0040] In addition, the skin cosmetic composition according to the present disclosure can be introduced into the skin by direct injection, in addition to being applied as an external agent to the surface of the skin as a filler composition.

[0041] Furthermore, while conventional skin care compositions contain various synthetic ingredients as active ingredients, which can cause negative effects such as inflammatory reactions when applied to sensitive skin, the skin care composition according to the present disclosure contains a natural ingredient, fish extract, thereby providing a function that improves the skin while being low-irritation and delivered to the deep layers of the skin.

[0042] The above skin beauty composition may have the effect of improving skin moisture and elasticity, preventing skin aging, and healing skin damage, and may be provided in various forms such as cream, lotion, essence, ampoule, and mask.

[0043] For example, the fish extract may be included in an amount of 0.1 to 3.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0044] When the above fish extract is included within the above numerical range, the efficacy of the fish extract can be fully exhibited while also showing low irritation to the skin. If the content of the fish extract exceeds the upper limit of the above numerical range, stickiness or allergic reactions may be induced on the skin, and if the content of the fish extract falls below the lower limit of the above numerical range, the skin improvement effect is negligible; therefore, it is desirable for the content of the fish extract to satisfy the above numerical range.

[0045] For example, the fish extract may be a component containing PDRN (polydeoxyribonucleotide), and the PDRN may be included in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total fish extract.

[0046] The above PDRN is a DNA component of salmon or trout and is a tissue regeneration component similar to human DNA that is also present in human cells; it is physiologically hypoallergenic and can perform the function of promoting the skin's inherent regeneration and metabolic activity. By including PDRN in the fish extract, the above can impart a skin regeneration effect to the skin cosmetic composition according to the present disclosure and improve skin elasticity and wrinkles. When the above PDRN is included within the above numerical range, it can exhibit hypoallergenic properties to the skin while fully demonstrating the efficacy of PDRN.

[0047] For example, the fish extract may be a component containing PN (polynucleotide), and the PN may be included in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total fish extract.

[0048] The above PN has a longer nucleic acid chain length than PDRN, resulting in a larger molecular size and molecular weight, through which it can perform the function of regenerating tissue and reducing inflammation. By including PN in the fish extract, the skin cosmetic composition according to the present disclosure can impart a skin regeneration effect and improve skin elasticity and wrinkles. When the above PN is included within the above numerical range, the efficacy of PN can be fully exerted while exhibiting low irritation to the skin.

[0049] For example, the fish extract is a fish extract extracted from salmon, and the fish extract may include an extract extracted from the fins and tail of salmon.

[0050] The fish extract derived from the salmon may include a polymer composed of nucleic acid-sugar-phosphate, and the polymer may have a structure substantially identical to PDRN or PN. The fish extract may have DNA repair effects, skin damage recovery effects, and wrinkle and pigmentation improvement effects, and may have antioxidant effects and further provide moisture and nutrients to the skin. Additionally, the fish extract derived from the salmon may be a component containing a water-soluble component obtained by extracting the salmon's sperm or testes, and if the fish extract includes an extract obtained from the salmon's fins and tail, the fish extract may increase the viscosity of the skin cosmetic composition according to the present disclosure by having high viscosity.

[0051] For example, the fish extract is a fish extract extracted from trout, and the fish extract may include an extract extracted from the fins and tail of the trout.

[0052] The fish extract obtained from the above-mentioned trout may include a polymer composed of nucleic acid-sugar-phosphate, and the polymer may have a structure substantially identical to PDRN or PN. The fish extract may have DNA repair effects, skin damage recovery effects, and wrinkle and pigmentation improvement effects, and may have antioxidant effects and further provide moisture and nutrients to the skin. Additionally, the fish extract obtained from the above-mentioned trout may be a component containing a water-soluble component obtained by extracting the sperm or testes of the trout, and if the fish extract includes an extract obtained from the fins and tail of the trout, the fish extract may increase the viscosity of the skin cosmetic composition according to the present disclosure by having high viscosity.

[0053] For example, the fish extract may consist of a first fish extract extracted from salmon and a second fish extract extracted from trout, wherein the first fish extract may include an extract extracted from the fins and tail of salmon, and the second fish extract may include an extract extracted from the fins and tail of trout.

[0054] By including the above fish extract together with the low molecular weight hyaluronic acid component included in the skin cosmetic composition according to the present disclosure, the fish extract can repair skin damage as it reaches the skin tissue, and the low molecular weight hyaluronic acid component can repair the tissue as it reaches the skin tissue, thereby providing a mutually complementary beneficial effect to the skin cosmetic composition according to the present disclosure.

[0055] For example, the low molecular weight hyaluronic acid may be included in an amount of 15 to 30 parts by weight based on 100 parts by weight of the total skin cosmetic composition.

[0056] Hyaluronic acid is a high-molecular-weight polysaccharide composed of D-glucuronic acid and N-acetyl glucosamine. It is an ingredient that provides excellent viscoelasticity and moisturizing power to the skin, increases the skin's moisture content and elasticity, has a skin tissue repair effect, and has the effect of absorbing external shocks to the skin.

[0057] For example, the low molecular weight hyaluronic acid may be a hyaluronic acid component having a molecular weight of 7 to 40 kDA and may have a pH of 6.3 to 6.7. The skin cosmetic composition according to the present disclosure can maximize the skin permeability of the hyaluronic acid component by including a low molecular weight hyaluronic acid component having a molecular weight within the above numerical range. If the molecular weight of the low molecular weight hyaluronic acid exceeds the upper limit of the above numerical range, the usability may deteriorate as skin permeability is insufficient and viscosity becomes higher than necessary. If the molecular weight of the low molecular weight hyaluronic acid falls below the lower limit of the above numerical range, the moisture content may be insufficient or an inflammatory reaction may occur. Therefore, it is desirable for the molecular weight of the low molecular weight hyaluronic acid to satisfy the above numerical range. In addition, the skin cosmetic composition according to the present disclosure includes a low molecular weight hyaluronic acid component having a pH within the above numerical range, thereby allowing the pH of the skin to be maintained while maintaining the stability and efficacy of the hyaluronic acid component. If the pH of the low molecular weight hyaluronic acid exceeds the upper limit of the above numerical range, the crosslinking ability of the hyaluronic acid component is reduced, which may result in the viscosity of the composition being too low. If the pH of the low molecular weight hyaluronic acid falls below the lower limit of the above numerical range, the hyaluronic acid component may decompose or irritate the skin. Therefore, it is preferable that the pH of the low molecular weight hyaluronic acid satisfy the above numerical range.

[0058] For example, the above skin cosmetic composition may additionally include allantoin. Allantoin is a naturally derived ingredient that can be extracted from plants such as comfrey and sugar beets, has a molecular structure and efficacy similar to urea, and is known to have effects such as exfoliation, moisturization, soothing, cell proliferation, wound healing, and anti-aging. By additionally including allantoin, the skin cosmetic composition according to the present disclosure can complement and enhance the beneficial effects of the fish extract and hyaluronic acid included in the composition, and, for example, can maximize the skin improvement effect resulting from the provision of the fish extract by removing the dead skin cells that hinder the introduction of the fish extract into the skin.

[0059] For example, the allantoin may be included in an amount of 0.1 to 1.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition. If the content of the allantoin exceeds the upper limit of the numerical range, the skin may become thin and dry, and if the content of the allantoin falls below the lower limit of the numerical range, the effect on the skin is negligible; therefore, it is desirable for the content of the allantoin to satisfy the numerical range.

[0060] For example, the above skin cosmetic composition may further include acetyl hexapeptide-8. Acetyl hexapeptide-8 is a small protein molecule that has the effect of inhibiting muscle contraction and can improve wrinkles by having an effect similar to Botox, and can maintain skin elasticity by inhibiting the loss of collagen and elastin.

[0061] For example, the acetyl hexapeptide-8 may be included in an amount of 0.01 to 0.1 parts by weight based on 100 parts by weight of the total skin cosmetic composition. If the content of the acetyl hexapeptide-8 exceeds the upper limit of the numerical range, stickiness may occur on the skin and it may become thin, and if the content of the acetyl hexapeptide-8 falls below the lower limit of the numerical range, the effect on the skin is negligible; therefore, it is desirable for the content of the acetyl hexapeptide-8 to satisfy the numerical range.

[0062] For example, the above skin cosmetic composition may additionally include jujube extract. The jujube extract contains vitamin C, antioxidants, minerals, etc., and has the efficacy of maintaining the moisture properties of the skin, reducing skin inflammation, and promoting blood circulation. Additionally, for example, the jujube extract may be included in an amount of 0.5 to 5.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition. If the content of the jujube extract exceeds the upper limit of the above numerical range, the heaviness or viscosity on the skin may increase, leading to reduced usability, and if the content of the jujube extract falls below the lower limit of the above numerical range, the effect on the skin is negligible; therefore, it is desirable for the content of the jujube extract to satisfy the above numerical range.

[0063] For example, the above skin cosmetic composition may additionally include a herbal mixture of *Clematis terniflora* extract, *Perilla frutescens* extract, *Ligustrum japonicum* extract, and *Ilex crenata* extract.

[0064] The above-mentioned *Byeongjoheepul* is a perennial herb belonging to the Asteraceae family, with a height of about 30 to 100 cm. Its leaves are alternate and oval-shaped, and its flowers bloom in white or light purple from July to September. Its fruits ripen in dark red from October to November and have the effect of improving dermatitis, hair loss, headaches, and indigestion.

[0065] The above-mentioned Soyeop is a perennial herb belonging to the Asteraceae family, with a height of about 30 to 80 cm. Its leaves are alternate and oval-shaped, and its flowers bloom in yellow in August and September. Its fruits ripen in brown in October and November and have the effect of improving various inflammations and blood circulation.

[0066] The above-mentioned *Jeolgukdae* is a perennial herb belonging to the Asteraceae family, with a height of about 50 to 150 cm. Its leaves are alternate and oval-shaped, and its flowers bloom in yellow from August to October. Its fruits ripen in brown from October to November and have the effect of improving blood flow and strengthening the elasticity of blood vessel walls.

[0067] The above holly is an evergreen broad-leaved tree belonging to the family Holly, with a height of about 5m. Its leaves are alternate and glossy, and its flowers bloom in white from April to May. Its fruits ripen in red from September to October and it has the effect of improving various inflammations in the body and reducing pain on irritated skin.

[0068] For example, the above herbal mixture may be included in an amount of 0.3 to 3.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition. If the content of the above herbal mixture exceeds the upper limit of the above numerical range, an allergic reaction or inflammation may occur on the skin, and if the content of the above herbal mixture falls below the lower limit of the above numerical range, the effect on the skin is negligible; therefore, it is desirable for the content of the above herbal mixture to satisfy the above numerical range.

[0069] For example, the above skin cosmetic composition may additionally include excipients, and said excipients may include sweeteners, flavoring agents, preservatives, acidity regulators, colorants, fragrances, etc.

[0070] The present disclosure is described in more detail below using examples. Process conditions and preparation steps not specified in the following examples may be obvious in the art to which the present disclosure belongs, and a person skilled in the art will be able to select them without difficulty based on the present disclosure and reproduce the problem-solving principles of the present disclosure.

[0071] In addition, regarding the manufacturing method according to the present disclosure, unless otherwise specified, each step constituting the manufacturing method is to be understood as being carried out at room temperature (25°C) and each step is to be performed by means and tools that a person skilled in the art can derive without particular difficulty.

[0072] Example 1: Preparation of a skin cosmetic composition comprising fish extract and hyaluronic acid

[0073] 5 kg each of salmon and trout were crushed and pressed to extract about 6 kg of liquid components, additional solid components were removed by filtration, 0.3 kg of magnesium sulfate was added to remove the fishy odor, and the composition was freeze-dried to obtain about 1.8 kg of a powder formulation. The powder formulation was mixed with water to prepare a fish extract according to the present disclosure.

[0074] Hyaluronic acid powder with an average molecular weight of 15 kDa was mixed with water to prepare an aqueous hyaluronic acid solution having a pH of approximately 6.5, and then mixed with the fish extract, water was added, and the mixture was stirred at 250 RPM for 20 minutes. Subsequently, a translucent, mucilaginous composition was obtained, which was subdivided to prepare the composition of Example 1. The content of each component used is shown in Table 1 below.

[0075] Examples 2 to 5: Preparation of skin cosmetic compositions according to the present disclosure

[0076] The composition of Example 2 was prepared in the same manner as described in Example 1, except that powdered allantoin (commercially available from Dyne) was additionally included in a mixture of fish extract and hyaluronic acid components, and then water was added.

[0077] The composition of Example 3 was prepared in the same manner as described in Example 2, except that powdered acetyl hexapeptide-8 (commercially available from C&K International) was additionally included in a mixture of fish extract and hyaluronic acid components, and then water was added.

[0078] A jujube extract was prepared by grinding dried jujube raw material, mixing it with water, heating it to 95 degrees Celsius for 3 hours, and removing the solids, and the composition of Example 4 was prepared in the same manner as described in Example 3, except that the jujube extract was additionally included in a mixture of fish extract and hyaluronic acid components and water was added.

[0079] A herbal mixture was prepared by preparing dried *Clematis terniflora*, dried *Perilla frutescens*, dried *Ilex crenata*, and dried *Ilex aquifolium*, cutting them, adding water to each, heating them at 95 degrees Celsius for 3 hours to remove solids, and then mixing them. The composition of Example 5 was prepared in the same manner as described in Example 4, except that the herbal mixture was additionally included in a mixture of fish extract and hyaluronic acid components, and then water was added.

[0080] The content of each component used in Examples 2 to 5 above is shown in Table 1 below.

[0081] Unit: parts by weight Examples 1 Examples 2 Examples 3 Examples 4 Examples 5 Examples 6 Comparative example fish extract 2.0 2.0 2.0 2.0 2.0 2.0 - Hyaluronic acid 20 20 20 20 20 20 22 allantoin - 0.5 0.5 0.5 0.5 - - Acetylhexapeptide-8 - - 0.05 0.05 0.05 - - Jujube extract - - - 3.0 3.0 3.0 - Byeongjoheepul extract - - - - 0.5 0.5 - Perilla leaf extract - - - - 0.5 0.5 - Morus alba extract - - - - 0.5 0.5 - Holly extract - - - - 0.5 0.5 - glycerin 5 5 5 5 5 5 5 Triethanolamine 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Propylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount entire 100 100 100 100 100 100 100

[0082] Experimental Example 1: Evaluation of skin moisturizing properties of a skin cosmetic composition according to the present disclosure

[0083] Among men in their 30s to 50s with dry skin or signs of skin aging on their left upper arms, 20 men were randomly selected and divided into 7 groups of 2 men each. The subjects in 6 of these groups were instructed to apply 3g of the compositions of Examples 1 to 5 and Comparative Example to an area of ​​5cm by 5cm at 09:00 and 22:00 daily, while the remaining 1 group was instructed not to apply any separate composition as a control group, and the experiment was conducted for 4 weeks. Before the experiment, the transepidermal water evaporation (TEWL) and skin moisture content (Corneometer) of the subjects were measured to establish baseline values.

[0084] Skin moisture evaporation and skin moisture content of the subjects were measured at 7, 14, 21, and 28 days from the start of the experiment, respectively. The results were averaged by group and compared with baseline values ​​to convert the degree of improvement into a percentage, which is shown in Tables 2 and 3 below, respectively.

[0085] unit: % Evaporation amount Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative example control group Week 1 10 9 12 15 19 14 3 2 Week 2 14 16 15 21 26 19 8 4 Week 3 18 22 23 24 28 22 11 5 Week 4 24 27 28 32 35 29 13 6

[0086] unit: % Moisture content Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative example control group Week 1 12 14 15 18 22 19 5 3 Week 2 18 19 18 22 27 25 6 5 Week 3 23 20 22 26 31 28 6 6 Week 4 24 25 29 32 39 37 9 7

[0087] Referring to Tables 2 and 3 above, it was confirmed that when the skin cosmetic composition according to the present disclosure is applied, the amount of moisture evaporation from the skin is continuously reduced and the amount of skin moisture is continuously increased. In particular, it was observed that the amount of skin moisture increases when jujube extract is additionally included, and it was confirmed that the amount of moisture evaporation from the skin is further reduced when a herbal mixture is additionally included.

[0088] Experimental Example 2: Evaluation of wrinkle-improving properties of a skin cosmetic composition according to the present disclosure

[0089] Among men in their 30s to 50s with dry skin or signs of skin aging on their left upper arms, 20 men were randomly selected and divided into 7 groups of 2 men each. The subjects in 6 of these groups were instructed to apply 3g of the compositions of Examples 1 to 5 and Comparative Example to an area 5cm by 5cm daily at 09:00 and 22:00, while the remaining 1 group was instructed not to apply any separate composition as a control group, and the experiment was conducted for 4 weeks. Prior to the experiment, the skin wrinkle condition of the subjects was measured using Primos CR (Canfield Scientific) to establish baseline values.

[0090] At 7, 14, 21, and 28 days from the start of the experiment, the skin wrinkle condition of the subjects was evaluated based on the value obtained by multiplying the total depth and length of the wrinkles, and the results were averaged by group and compared with the baseline value to convert the degree of improvement into a percentage unit, which is shown in Table 4 below.

[0091] unit: % Wrinkle characteristics Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative example control group Week 1 11 18 20 25 29 21 2 1 Week 2 15 22 22 32 37 30 4 1 Week 3 25 27 29 36 41 33 7 2 Week 4 27 31 33 39 45 36 12 2

[0092] Referring to Table 4 above, it was confirmed that when the skin cosmetic composition according to the present disclosure is applied, wrinkles on the skin are continuously improved. In particular, it was confirmed that the degree of wrinkle improvement is further increased when allantoin is additionally included, and it was also confirmed that the degree of wrinkle improvement is further increased when acetyl hexapeptide-8 is additionally included.

[0093] Experimental Example 3: Evaluation of cytotoxicity of a skin cosmetic composition according to the present disclosure

[0094] Human dermal fibroblasts (NHDF) 2×10 5The compositions were dispensed into 6-well culture plates (commercially available from Corning) at a concentration of cells / well and cultured in an incubator at 37°C. After 48 hours, the compositions of Examples 1 to 5 and the Comparative Example were placed in the center of the culture dish to bring into contact with the cells, with a diameter of approximately 1 cm, and cultured for 24 hours. Each composition and the cultured cells were stained with hematoxylin (commercially available from Sigma), and the toxicity response area was calculated using ImageJ software. The degree of toxicity was evaluated based on the size of the toxicity response area according to the criteria listed in Table 18 below (MCIM Amin, N. Ahmad, N. Halib, and I. Ahmad, Carbohydr. Polym., 88, 465, 2012), and the results are shown in Table 5 below. Here, a lower grade indicates lower cytotoxicity of the composition.

[0095] Unit: Grade Example 1 Example 2 Example 3 Example 4 Example 5 Comparative example cytotoxicity 1 1 1 1 1 1

[0096] Referring to Table 5 above, it was confirmed that all compositions of Examples 1 to 5 according to the present disclosure are substantially free of cytotoxicity, thus confirming that the skin cosmetic composition according to the present disclosure will not have a negative effect on the skin even when applied to the skin.

[0097] Although preferred embodiments of the present disclosure have been described in detail above, the scope of the present disclosure is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concepts of the present disclosure as defined in the following claims are also included within the scope of the present disclosure.

Claims

Claim 1 A skin cosmetic composition comprising a fish extract and hyaluronic acid, wherein the fish extract is a fish extract extracted from salmon and / or trout, and the hyaluronic acid is a low molecular weight hyaluronic acid component having a molecular weight of 5 to 50 kDa, wherein the fish extract is included in an amount of 0.1 to 3.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition, wherein the fish extract comprises PDRN (polydeoxyribonucleotide), wherein the PDRN is included in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total fish extract, wherein the fish extract comprises PN (polynucleotide), wherein the PN is included in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total fish extract, and wherein the fish extract is composed of a first fish extract extracted from salmon and a second fish extract extracted from trout. The first fish extract comprises an extract obtained from the fins and tail of salmon, and the second fish extract comprises an extract obtained from the fins and tail of trout, and the low molecular weight hyaluronic acid is included in an amount of 15 to 30 parts by weight based on 100 parts by weight of the total skin cosmetic composition, and the low molecular weight hyaluronic acid is a hyaluronic acid component having a molecular weight of 7 to 40 kDa and a pH of 6.3 to 6.7, and the skin cosmetic composition further comprises allantoin, and the allantoin is included in an amount of 0.1 to 1.0 parts by weight based on 100 parts by weight of the total skin cosmetic composition, and the skin cosmetic composition further comprises acetyl hexapeptide-8, and the acetyl hexapeptide-8 is included in an amount of 0.01 to 0.1 parts by weight based on 100 parts by weight of the total skin cosmetic composition, and the skin cosmetic composition further comprises jujube extract, and the Jujube extract is included in an amount of 0.5 to 5.0 parts by weight based on 100 parts by weight of the total skin beauty composition, and the skin beauty composition further includes a herbal mixture of *Clematis terniflora* extract, *Perilla frutescens* extract, *Ligustrum japonicum* extract, and *Ilex crenata* extract, and the herbal mixture is 0. based on 100 parts by weight of the total skin beauty composition.A skin cosmetic composition comprising 3 to 3.0 parts by weight.

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