SNP markers and primer sets for high-throughput identification of apple cultivar and uses thereof

Abstract

This invention relates to an SNP marker and primer set for identifying apple varieties and the use thereof. By using the SNP marker and primer set, a large number of apple varieties can be identified at a lower cost and higher efficiency than existing methods. This can be utilized in seed distribution management tasks such as seed variety protection applications and prevention of illegal distribution of seeds, and can also contribute to fields such as verifying the authenticity of apple varieties and mediating seed disputes.

Description

Technology Field

[0001] This invention relates to SNP markers, a primer set, and their applications for high-efficiency identification of a large number of apple varieties. Background Technology

[0002] Genetic markers refer to specific nucleotide sequence fragments that can determine the presence of polymorphism in the genome. In the field of crop breeding, genetic markers are utilized in areas such as the development of trait-associated markers, marker-assisted selection (MAS) based on these markers, gene mapping, quantitative trait locus analysis, purity testing, genetic diversity analysis, and variety identification. Generally, methods for identifying plant varieties are broadly categorized into morphological characteristic testing through cultivation trials, testing based on differences in isoenzymes between varieties, and DNA analysis. Among these, DNA analysis offers the advantage of being objective and highly reproducible compared to phenotype-based identification methods, as it is not affected by the cultivation environment or the crop's growth stage.

[0003] Various studies suggest the possibility of utilizing differences in nucleotide sequences at the genetic level as molecular markers for variety identification. Specifically, among the types of molecular markers, it is suggested to utilize Simple Sequence Repeats (SSRs), Single Nucleotide Polymorphisms (SNPs), and Indels, which can best express crop polymorphisms when considering the degree of polymorphism between varieties, the reproducibility of analysis, and the degree of distribution on chromosomes. Korean Registered Patent No. 0974821 discloses an SNP marker for identifying commercially available F1 pepper varieties.

[0004] As such, research on differences between individuals using genetic material has been steadily conducted, and with the recent advancements in molecular genetics and DNA analysis technology, the development of genetic markers including SNPs, such as the search for genes related to economic traits and the discovery of genes related to diseases at the molecular biological level, is actively underway.

[0005] Meanwhile, apple (scientific name: Malus domestica , Malus pumila Apples are an economically important temperate crop due to their high global production. Morphological and physiological characteristics of apples have been used as tools for identifying apple varieties. However, these methods have disadvantages, such as being time-consuming, expensive, and often based on subjective decisions (Cooke RJ (1995), Varietal identification of crop plants, p.33-63). Since multigenic, quantitative, and environmental factors influence the expression of apple variety characteristics, the research group on biochemical and molecular biological techniques at the International Union for the Protection of New Varieties of Plants (UPOV) is calling for the use of molecular biological primers for apple variety identification.

[0006] Against this backdrop, the inventors have discovered a genetic marker capable of identifying apple varieties and a primer set capable of detecting said genetic marker, and introduced them into the KASP (Kompetitive Allele-Specific PCR) analysis method to rapidly and economically identify a large number of apple varieties. The discovered marker and primer set can be usefully utilized not only for identifying apple varieties but also for the protection of apple varieties and genetic resources. The problem to be solved

[0007] One aspect is to provide a set of primers for apple variety identification.

[0008] Another aspect is to provide a kit for identifying apple varieties that includes the above-mentioned primer set.

[0009] Another aspect is providing a method to identify apple varieties.

[0010] Other objects and advantages of this application will become more apparent from the following detailed description, together with the appended claims and drawings. Anything not described in this specification is omitted, as it can be sufficiently recognized and inferred by those skilled in the art of this application or a similar art field. means of solving the problem

[0011] Each description and embodiment disclosed in this application may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this application fall within the scope of this application. Furthermore, the scope of this application should not be considered limited by the specific descriptions provided below.

[0013] One aspect provides a set of primers for apple variety identification (or discrimination) comprising each set of primers for detecting a single nucleotide polymorphism (SNP) located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96.

[0014] The term "polymorphism" refers to the presence of two or more alleles at a single locus, and among polymorphisms, when only a single nucleotide differs between individuals is called a single nucleotide polymorphism (SNP).

[0015] The term "allele" refers to various variations of a given gene. For example, it can refer to multiple types of a single gene located at the same locus on homologous chromosomes. Additionally, alleles are used to describe polymorphism; for instance, an SNP can have two types of alleles.

[0016] The term "single nucleotide polymorphism (SNP)" refers to a base variation within a chromosomal sequence that indicates a deviation between individuals or varieties. Specifically, it may refer to a difference of a single base (or a single base pair) within a specific sequence. Single nucleotide polymorphisms are known to occur with the highest frequency among polymorphisms in DNA sequences. Utilizing this characteristic, the present invention selected SNPs for identifying apple varieties, and among the selected SNPs, identified SNP markers that demonstrated discriminative power and excellent results in specificity and sensitivity, and used them for apple variety identification.

[0017] The term "marker" or "genetic marker" refers to a nucleotide sequence used as a reference when identifying genetically unrelated loci, and the term "locus" refers to the position of a molecular marker on a gene map.

[0018] The above single nucleotide polymorphisms may refer to all single nucleotide polymorphisms located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96. The above single nucleotide polymorphisms may be associated with genes that indicate differences between apple varieties. The above single nucleotide polymorphisms may be nucleotide variations that indicate differences between apple varieties. Therefore, the above single nucleotide polymorphisms may function as genetic markers capable of identifying apple varieties.

[0019] Specifically, the single nucleotide polymorphism is a variation that occurs at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96, and the nucleotide at said position may have two types of alleles (i.e., one of two types of nucleotides), and said alleles may be as described in the 'SNP nucleotide (allele)' item corresponding to each of SEQ ID NOs 1 to 96 in Table 1 below. More specifically, the single nucleotide polymorphism is a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 1 is G or A, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 2 is A or G, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 3 is G or T, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 4 is C or T, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 5 is T or C, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 6 is A or C, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 7 is A or T, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 8 is T or C, a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 9 is A or C, and a polymorphism in which the 201st base in the nucleotide sequence of SEQ ID NO. 10 is G or polymorphism in which the 201st base in the sequence of SEQ ID NO. 11 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 12 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 13 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 14 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 15 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 16 is C or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 17 is A or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 18 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 19 is C or T,Polymorphism in which the 201st base in the sequence of SEQ ID NO. 20 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 21 is C or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 22 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 23 is T or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 24 is A or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 25 is G or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 26 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 27 is G or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 28 is A or T, and in which the 201st base in the sequence of SEQ ID NO. 29 is A or C Polymorphism, polymorphism in which the 201st base in the sequence of SEQ ID NO. 30 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 31 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 32 is T or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 33 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 34 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 35 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 36 is A or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 37 is T or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 38 is G or A, the 201st base in the sequence of SEQ ID NO. 39 is Polymorphism in which the 201st base in the sequence of SEQ ID NO. 40 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 41 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 42 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 43 is A or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 44 is A or T,Polymorphism in which the 201st base in the sequence of SEQ ID NO. 45 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 46 is C or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 47 is G or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 48 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 49 is G or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 50 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 51 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 52 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 53 is G or T, and in which the 201st base in the sequence of SEQ ID NO. 54 is T or C Polymorphism, polymorphism in which the 201st base in the sequence of SEQ ID NO. 55 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 56 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 57 is G or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 58 is C or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 59 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 60 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 61 is C or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 62 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 63 is C or A, the 201st base in the sequence of SEQ ID NO. 64 is Polymorphism in which the 201st base in the sequence of SEQ ID NO. 65 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 66 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 67 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 68 is C or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 69 is T or A,Polymorphism in which the 201st base in the sequence of SEQ ID NO. 70 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 71 is C or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 72 is T or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 73 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 74 is T or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 75 is C or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 76 is G or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 77 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 78 is C or T, and in which the 201st base in the sequence of SEQ ID NO. 79 is A or G Polymorphism, polymorphism in which the 201st base in the sequence of SEQ ID NO. 80 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 81 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 82 is T or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 83 is A or T, polymorphism in which the 201st base in the sequence of SEQ ID NO. 84 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 85 is T or A, polymorphism in which the 201st base in the sequence of SEQ ID NO. 86 is A or C, polymorphism in which the 201st base in the sequence of SEQ ID NO. 87 is A or G, polymorphism in which the 201st base in the sequence of SEQ ID NO. 88 is C or G, the 201st base in the sequence of SEQ ID NO. 89 is Polymorphism in which the 201st base is C or A in the sequence of SEQ ID NO. 90, polymorphism in which the 201st base is C or G in the sequence of SEQ ID NO. 91, polymorphism in which the 201st base is T or C in the sequence of SEQ ID NO. 92, polymorphism in which the 201st base is A or T in the sequence of SEQ ID NO. 93, polymorphism in which the 201st base is T or A in the sequence of SEQ ID NO. 94,It may be a polymorphism in which the 201st base in the base sequence of SEQ ID NO. 95 is C or T, and a polymorphism in which the 201st base in the base sequence of SEQ ID NO. 96 is C or T.

[0020] Each primer set for detecting the above single nucleotide polymorphisms may detect only one corresponding single nucleotide polymorphism among the above single nucleotide polymorphisms. That is, the primer set for apple variety identification may detect all of the above single nucleotide polymorphisms by including all of the primer sets corresponding to each of the above single nucleotide polymorphisms for detecting each of the above single nucleotide polymorphisms. Accordingly, the primer set for apple variety identification may be for detecting all of the above single nucleotide polymorphisms.

[0021] Each primer set for detecting the above single nucleotide polymorphism may amplify a (polymorphic) region (i.e., a nucleotide sequence region containing the nucleotide at the corresponding position) containing the nucleotide at a single nucleotide polymorphism position corresponding to the above single nucleotide polymorphism. Accordingly, the primer set for identifying apple varieties may include (all) each primer set for amplifying each nucleotide sequence containing the nucleotide at each single nucleotide polymorphism position. The region containing the nucleotide at each single nucleotide polymorphism position amplified by each primer set may be a sequence region composed of five or more, eight or more, or ten or more consecutive nucleotides containing the nucleotide at each single nucleotide polymorphism position, or a sequence region complementary thereto. For example, the region containing the base at each single nucleotide polymorphism position amplified by each primer set may be each sequence region or each sequence region complementary thereto, composed of five or more, eight or more, or ten or more consecutive nucleotides containing the 201st base of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96. Accordingly, each primer set for detecting the single nucleotide polymorphism may be designed and manufactured according to general methods known in the art to amplify each sequence region containing the single nucleotide polymorphism base located at the 201st position within each nucleotide sequence, based on each of the nucleotide sequences of SEQ ID NOs 1 to 96.

[0022] According to one embodiment, each primer set for detecting the single nucleotide polymorphism comprises: a first primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 97 to 100; a second primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 101 to 104; a third primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 105 to 108; a fourth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 109 to 112; a fifth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 113 to 116; a sixth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 117 to 120; a seventh primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 121 to 124; and an eighth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 125 to 128. A ninth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 129 to 132; a tenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 133 to 136; an eleventh primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 137 to 140; a twelfth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 141 to 144; a thirteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 145 to 148; a fourteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 149 to 152; a fifteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 153 to 156; and a sixteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 157 to 160.A 17th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 161 to 164; an 18th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 165 to 168; a 19th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 169 to 172; a 20th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 173 to 176; a 21st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 177 to 180; a 22nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 181 to 184; a 23rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 185 to 188; a 24th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 189 to 192; A 25th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 193 to 196; a 26th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 197 to 200; a 27th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 201 to 204; a 28th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 205 to 208; a 29th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 209 to 212; a 30th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 213 to 216; a 31st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 217 to 220; a 32nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 221 to 224; A 33rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 225 to 228;A 34th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 229 to 232; a 35th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 233 to 236; a 36th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 237 to 240; a 37th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 241 to 244; a 38th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 245 to 248; a 39th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 249 to 252; a 40th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 253 to 256; a 41st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 257 to 260; A 42nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 261 to 264; a 43rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 265 to 268; a 44th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 269 to 272; a 45th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 273 to 276; a 46th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 277 to 280; a 47th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 281 to 284; a 48th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 285 to 288; a 49th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 289 to 292; A 50th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 293 to 296;A 51st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 297 to 300; a 52nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 301 to 304; a 53rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 305 to 308; a 54th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 309 to 312; a 55th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 313 to 316; a 56th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 317 to 320; a 57th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 321 to 324; a 58th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 325 to 328; A 59th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 329 to 332; a 60th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 333 to 336; a 61st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 337 to 340; a 62nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 341 to 344; a 63rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 345 to 348; a 64th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 349 to 352; a 65th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 353 to 356; a 66th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 357 to 360; Primer set 67 consisting of four types of oligonucleotides represented by SEQ ID NOs 361 to 364;Primer set 68 consisting of four types of oligonucleotides represented by SEQ ID NOs 365 to 368; Primer set 69 consisting of four types of oligonucleotides represented by SEQ ID NOs 369 to 372; Primer set 70 consisting of four types of oligonucleotides represented by SEQ ID NOs 373 to 376; Primer set 71 consisting of four types of oligonucleotides represented by SEQ ID NOs 377 to 380; Primer set 72 consisting of four types of oligonucleotides represented by SEQ ID NOs 381 to 384; Primer set 73 consisting of four types of oligonucleotides represented by SEQ ID NOs 385 to 388; Primer set 74 consisting of four types of oligonucleotides represented by SEQ ID NOs 389 to 392; Primer set 75 consisting of four types of oligonucleotides represented by SEQ ID NOs 393 to 396; A 76th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 397 to 400; a 77th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 401 to 404; a 78th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 405 to 408; a 79th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 409 to 412; an 80th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 413 to 416; an 81st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 417 to 420; an 82nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 421 to 424; and an 83rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 425 to 428; A 84th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 429 to 432;A primer set of 85 consisting of four types of oligonucleotides represented by SEQ ID NOs 433 to 436; a primer set of 86 consisting of four types of oligonucleotides represented by SEQ ID NOs 437 to 440; a primer set of 87 consisting of four types of oligonucleotides represented by SEQ ID NOs 441 to 444; a primer set of 88 consisting of four types of oligonucleotides represented by SEQ ID NOs 445 to 448; a primer set of 89 consisting of four types of oligonucleotides represented by SEQ ID NOs 449 to 452; a primer set of 90 consisting of four types of oligonucleotides represented by SEQ ID NOs 453 to 456; a primer set of 91 consisting of four types of oligonucleotides represented by SEQ ID NOs 457 to 460; a primer set of 92 consisting of four types of oligonucleotides represented by SEQ ID NOs 461 to 464; It may be a 93rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 465 to 468; a 94th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 469 to 472; a 95th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 473 to 476; and a 96th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 477 to 480. According to one embodiment, the primer set for apple variety identification may include a combination of the 1st to 96th primer sets.

[0023] The term "nucleotide" refers to the basic unit of DNA or RNA, the genetic material of all living organisms, composed of three elements: a base, a pentose sugar, and a phosphate. There are two types of bases: purines and pyrimidines, and deoxyribose is used for DNA and ribose for RNA. Unless otherwise specifically noted, the above nucleotides may include analogs of natural nucleotides.

[0024] The term "polynucleotide or oligonucleotide" refers to a polymer of nucleotides in which nucleotide monomers are linked together in a chain by covalent bonds. Polymers of nucleotides can be divided into two types based on their length: oligonucleotides and polynucleotides. The aforementioned oligonucleotides generally refer to relatively short nucleotide polymers composed of fewer than tens of nucleotides, while the aforementioned polynucleotides refer to relatively long polymers composed of hundreds or thousands or more nucleotides, but are not limited thereto. The terms "oligonucleotide" and "polynucleotide" may be used interchangeably.

[0025] The term "primer" means a single-stranded polynucleotide or oligonucleotide that can act as a starting point in the polymerization of nucleotides by a polymerase. The primer may be prepared using methods known to a person skilled in the art. The primer may be an oligo-nucleotide primer for a polymerase chain reaction (PCR).

[0026] The sequence of each of the above primers may be identical to or complementary to some consecutive bases within any one of the base sequences of SEQ ID NOs 1 to 96. The length of the above primers may vary depending on various factors, such as temperature and the application of the primers. For example, in the case of a primer set for HRM (High Resolution Melting) analysis, it may be a primer set capable of producing amplification products of a size suitable for HRM analysis (e.g., about 50 to 500 bp).

[0027] The above primer set can be used for DNA amplification or single nucleotide polymorphism detection, and preferably for the amplification of genetic markers for apple variety identification or for the detection of single nucleotide polymorphisms. Accordingly, the above primer set can be used to identify or distinguish apple varieties.

[0028] According to one embodiment, each of the first to 96 primer sets may detect or identify a single nucleotide polymorphism located at the 201st base of any one selected among the nucleotide sequences of SEQ ID NOs 1 to 96 of Table 1 below. For example, the first primer set may detect or identify a single nucleotide polymorphism when the base located at the 201st of SEQ ID NO. 1 is G (guanine) or A (adenine), and the fourth primer set may detect or identify a single nucleotide polymorphism when the base located at the 201st of SEQ ID NO. 4 is C (cytosine) or T (thymine). The 'Sequence Number' item in Table 1 below indicates the sequence number in the sequence list attached to this specification, which describes the DNA sequence of each SNP site, the 'SNP Position in Sequence' item indicates the position of the SNP in the sequence of the sequence number, and the 'SNP Base (Allele)' item indicates the base exhibiting polymorphism at the SNP position. As described above, each single nucleotide polymorphism located at the 201st base of each base sequence in the base sequences of Sequence Numbers 1 to 96 of Table 1 below, which can be detected by each of the primer sets, may be associated with a gene exhibiting differences between apple varieties, and specifically, may be a base variant exhibiting differences between apple varieties.

[0029] Specifically, each of the first to 96 primer sets may identify the type (i.e., allele) of each base at a single nucleotide polymorphism position corresponding in Table 1 below. For example, the first primer set may identify whether the type (i.e., allele) of the base located at the 201st position of SEQ ID NO. 1 is G or A. More specifically, each of the first to 96 primer sets may identify the genotype of each base at a single nucleotide polymorphism position corresponding in Table 1 below. For example, the first primer set may identify whether the genotype of the base located at the 201st position of SEQ ID NO. 1 (i.e., a single base pair located at the same locus on a homologous chromosome) is GG (homozygote), GA (heterozygote), and AA (homozygote).

[0030] Accordingly, by using all of the above 1 to 96 primer sets to detect or identify all of the single nucleotide polymorphisms in Table 1 below, which are genetic markers capable of identifying apple varieties, apple varieties can be identified.

[0031] Primer set Detected SNPs Primer set Detected SNPs Sequence number SNP location in the sequence SNP bases (Allele) Sequence number SNP location in the sequence SNP bases (Allele) 1st Primer Set 1 201st G or A No. 49 Primer Set 49 201st G or C 2nd Primer Set 2 201st A or G No. 50 Primer Set 50 201st T or C 3rd Primer Set 3 201st G or T No. 51 Primer Set 51 201st C or T 4th Primer Set 4 201st C or T No. 52 Primer Set 52 201st A or G Primer Set No. 5 5 201st T or C No. 53 Primer Set 53 201st G or T Primer Set No. 6 6 201st A or C No. 54 Primer Set 54 201st T or C 7th Primer Set 7 201st A or T No. 55 Primer Set 55 201st C or T No. 8 Primer Set 8 201st T or C No. 56 Primer Set 56 201st A or G No. 9 Primer Set 9 201st A or C No. 57 Primer Set 57 201st G or T Primer Set No. 10 10 201st G or A No. 58 Primer Set 58 201st C or A Primer Set No. 11 11 201st A or G No. 59 Primer Set 59 201st C or T Primer Set No. 12 12 201st G or A No. 60 Primer Set 60 201st T or C The 13th Primer Set 13 201st T or C No. 61 Primer Set 61 201st C or A The 14th Primer Set 14 201st C or T No. 62 Primer Set 62 201st A or G The 15th Primer Set 15 201st G or A No. 63 Primer Set 63 201st C or A Primer Set No. 16 16 201st C or A No. 64 Primer Set 64 201st C or T The 17th Primer Set 17 201st A or C No. 65 Primer Set 65 201st C or T No. 18 Primer Set 18 201st C or T No. 66 Primer Set 66 201st T or C No. 19 Primer Set 19 201st C or T No. 67 Primer Set 67 201st A or G No. 20 Primer Set 20 201st A or G No. 68 Primer Set 68 201st C or A No. 21 Primer Set 21 201st C or A No. 69 Primer Set 69 201st T or A No. 22 Primer Set 22 201st C or T No. 70 Primer Set 70 201st G or A No. 23 Primer Set 23 201st T or A No. 71 Primer Set 71 201st C or A The 24th Primer Set 24 201st A or C No. 72 Primer Set 72 201st T or A No. 25 Primer Set 25 201st G or C No. 73 Primer Set 73 201st A or G No. 26 Primer Set 26 201st G or A No. 74 Primer Set 74 201st T or A No. 27 Primer Set 27 201st G or C No. 75 Primer Set 75 201st C or T No. 28 Primer Set 28 201st A or T No. 76 Primer Set 76 201st G or A No. 29 Primer Set 29 201st A or C No. 77 Primer Set 77 201st A or G No. 30 Primer Set 30 201st C or T No. 78 Primer Set 78 201st C or T No. 31 Primer Set 31 201st C or T No. 79 Primer Set 79 201st A or G No. 32 Primer Set 32 201st T or A No. 80 Primer Set 80 201st T or C No. 33 Primer Set 33 201st G or A No. 81 Primer Set 81 201st A or G The 34th Primer Set 34 201st G or A No. 82 Primer Set 82 201st T or C The 35th Primer Set 35 201st A or G No. 83 Primer Set 83 201st A or T No. 36 Primer Set 36 201st A or C No. 84 Primer Set 84 201st A or G No. 37 Primer Set 37 201st T or A No. 85 Primer Set 85 201st T or A No. 38 Primer Set 38 201st G or A No. 86 Primer Set 86 201st A or C No. 39 Primer Set 39 201st G or T No. 87 Primer Set 87 201st A or G No. 40 Primer Set 40 201st T or C No. 88 Primer Set 88 201st C or G No. 41 Primer Set 41 201st A or G No. 89 Primer Set 89 201st C or A No. 42 Primer Set 42 201st A or G No. 90 Primer Set 90 201st C or G No. 43 Primer Set 43 201st A or C No. 91 Primer Set 91 201st C or G No. 44 Primer Set 44 201st A or T No. 92 Primer Set 92 201st T or C No. 45 Primer Set 45 201st G or A No. 93 Primer Set 93 201st A or T No. 46 Primer Set 46 201st C or G No. 94 Primer Set 94 201st T or A No. 47 Primer Set 47 201st G or T No. 95 Primer Set 95 201st C or T No. 48 Primer Set 48 201st G or A No. 96 Primer Set 96 201st C or T

[0032] The polynucleotide or oligonucleotide of the primer may include nucleotide analogs such as phosphorothioates, alkylphosphorothioates, peptide nucleic acids, or intercalations. The polynucleotide or oligonucleotide of the primer may further include a labeling material that emits fluorescence, phosphorescence, or radioactivity. The labeling material may be labeled at the 5' end or 3' end of the polynucleotide or oligonucleotide. The fluorescent labeling material may be VIC, NED, FAM, PET, or a combination thereof. Additionally, the radioactive labeling material may be incorporated into the amplification product through a PCR reaction using a PCR reaction solution to which a radioisotope such as 32P or 35S has been added. The primer set may be a primer set for Fluidigm analysis. Fluidigm analysis allows for the rapid detection of two allele-type SNPs in large quantities. The primer set for the above-described Fluidigm analysis may include an LSP (Locus Specific Primer), ASP (Allele Specific Primer) 1, and ASP 2, and optionally include a STA (Specific Target Amplification Primer). The STA and LSP may be used for amplifying a target sequence containing an SNP, the LSP and ASP may be used to identify the SNP location, and ASP 1 and ASP 2 may be used to detect each of the SNP alleles. For the Fluidigm analysis, probes linked to fluorescent labels such as FAM or HEX may be used as probes. For example, each probe linked to FAM or HEX may be linked to ASP 1 and ASP 2, respectively.For example, ASP 1 and ASP 2 are specifically hybridized to the two alleles of the SNP, respectively, and the resulting product is amplified, and the probe sequences connected to ASP 1 and ASP 2 are co-amplified, and as a result, the fluorescence of the fluorescent label connected to the amplified probe is emitted, allowing the genotype of the SNP to be identified.

[0033] The above ASP 1 and ASP 2 may be constructed corresponding to the alleles of a single nucleotide polymorphism. For example, the 5' end or the 3' end of the above ASP 1 and ASP 2 may each have a nucleotide identical to or complementary to the allele of the single nucleotide polymorphism to be analyzed. For example, the above ASP 1 and ASP 2 may be constructed to consist of about 10 to 40 consecutive sequences or sequences complementary thereto, with the nucleotide at the SNP location in a nucleotide sequence containing the SNP as the 3' end, and at the 3' end of each of the above ASP 1 and ASP 2, one of the two types of nucleotides that are alleles of the SNP or two types of nucleotides complementary thereto may be located therein. The above STA may be constructed from approximately 10 to 40 consecutive sequences or complementary sequences among the forward (or backward) sequences of the SNP site in a sequence containing the SNP, and the above LSP may be constructed from approximately 10 to 40 consecutive sequences or complementary sequences among the backward (or forward) sequences of the SNP site in a sequence containing the SNP. The above oligonucleotide primers for Fluidigm analysis may be manufactured by ordering from Fluidigm Inc.

[0034] The above-described primer set for identifying apple varieties can identify one or more of the apple varieties listed in Table 2 below. According to one embodiment, it was confirmed that the above-described primer set for identifying apple varieties can identify 72 general apple varieties (Nos. 1 to 72 in Table 2 below) with 100% accuracy, excluding 14 Fuji bud mutation varieties and 3 Hongro bud mutation varieties among the 89 apple varieties in Table 2 below, and it was confirmed that while identification between bud mutation varieties is impossible, identification of whether a variety corresponds to a bud mutation variety is possible. Therefore, according to one embodiment, the above-described primer set for identifying apple varieties can distinguish between general apple varieties and bud mutation varieties, and can identify one or more of the apple varieties Nos. 1 to 72 in Table 2 below.

[0035] The term "bud mutant" refers to a branch or stem that has grown from a mutation in the genes of a plant's growth point. Leaves, flowers, and fruits growing on this mutant may differ in characteristics from those grown on ordinary branches. If beneficial mutations exist, these mutants can be propagated and utilized as bud mutant varieties.

[0036] Therefore, the term "bud mutation variety" may refer to a variety grown and harvested from a bud mutation site.

[0037] Another aspect provides a kit for identifying apple varieties comprising the above-described set of primers for identifying apple varieties. Specifically, the kit may comprise all of the above-described sets of primers 1 through 96. The kit may be able to detect or identify all single nucleotide polymorphisms located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 through 96, and thereby identify apple varieties.

[0038] The above kit can identify one or more of the apple varieties listed in Table 2 below. According to one embodiment, the kit distinguishes between common apple varieties and bud mutation varieties, and can identify one or more of the apple varieties numbered 1 to 72 in Table 2 below.

[0039] According to one embodiment, the kit may be a PCR kit or a kit for fluidigm analysis.

[0040] The above PCR kit or Fluidigm analysis kit may additionally include, but is not limited to, components necessary for the identification (or detection) of PCR or amplification products, such as DNA polymerase, a dNTP mixture (dATP, dCTP, dGTP, and dTTP), PCR buffer solution, a DNase inhibitor, a restriction enzyme, or sterile water. The above DNA polymerase is, for example, E. coli The DNA polymerase I "Klenow fragment," heat-stable DNA polymerase, reverse transcriptase such as RNA-dependent DNA polymerase, Taq polymerase, or bacteriophage T7 DNA polymerase may be, but is not limited thereto. The PCR buffer solution may contain buffer components such as KCl, Tris-HCl, or MgCl2, and may, for example, contain a PCR buffer. The restriction enzyme may be one or more of BfaI or BsaAI, but is not limited thereto. The PCR kit or Fluidigm analysis kit may include an instruction manual. The instruction manual may be a printed document explaining the use of the kit, for example, the method of preparing the PCR buffer solution, the presented reaction conditions, etc.

[0041] The above kit may comprise a composition for identifying apple varieties that includes the above-described set of primers for identifying apple varieties. In addition to the above-described set of primers for identifying apple varieties, the composition may further include components necessary for the identification (or detection) of PCR or amplification products as described above.

[0042] According to one embodiment, the kit may include all of the first to 96 primer sets, wherein the first to 96 primer sets included in the kit may all be contained in a single container, tube, well, or composition (e.g., the apple variety identification composition), or one or more primer sets selected from the first to 96 primer sets may be included in each of two or more independent containers, tubes, wells, or compositions. Furthermore, when two or more types of primer sets are included in a single container, tube, well, or composition, the combination of the included primer sets is not limited, and an appropriate combination among the first to 96 primer sets may be selected. That is, as long as the kit includes all of the first to 96 primer sets, the number of independent containers, tubes, wells, or compositions and the types of primer sets included in each container, tube, well, or composition may be appropriately determined by considering the purpose of analysis, analysis efficiency, etc.

[0043] The above kit or composition may further include reagents, instruments, or devices necessary to detect or identify the single nucleotide polymorphism using the above set of primers for apple variety identification, such as reagents, instruments, or devices necessary to perform PCR, DNA-DNA hybridization, fluorescence detection, etc.

[0044] Among the terms or elements mentioned in the above kit, those that are the same as those mentioned in the description of the primer set are understood to be the same as those mentioned above in the description of the primer set.

[0045] Another aspect provides a method for identifying apple varieties comprising: (a) a step of isolating genomic DNA from an apple; (b) a step of detecting (all) single nucleotide polymorphisms located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96 in the isolated genomic DNA; and (c) a step of identifying apple varieties by analyzing the detection results.

[0046] The above genomic DNA may be derived from the leaves, stems, fruits, flowers, roots, or a combination thereof of an apple. The above genomic DNA may be obtained using conventional methods for extracting DNA from a plant.

[0047] Specifically, in step (b) above, all single nucleotide polymorphism positions located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96 may be detected. For example, in step (b) above, all alleles (i.e., nucleotide types) existing at the corresponding positions for all single nucleotide polymorphisms may be detected. That is, in step (b) above, the presence or absence of a specific type of nucleotide at the positions of all single nucleotide polymorphisms may be detected. For example, in step (b) above, the presence or absence of allele bases present at all single nucleotide polymorphism positions in Table 3 below may be detected (e.g., for single nucleotide polymorphism 1 in Table 3 below, the presence or absence of the allele base G or A present at the corresponding position is detected), and in this case, for each single nucleotide polymorphism, the allele present at the corresponding position may be detected by distinguishing according to base type (e.g., for single nucleotide polymorphism 1 in Table 3 below, the case where only G is present, the case where only A is present, or the case where both G and A are present are detected by distinguishing respectively).

[0048] In step (b) above, probes, primers, or restriction enzymes prepared to detect a single nucleotide polymorphism located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96 may be used, but are not limited thereto.

[0049] For example, the probe may be a polynucleotide (oligonucleotide) or a polynucleotide (oligonucleotide) complementary thereto, comprising a sequence of five or more, eight or more, or ten or more consecutive nucleotides including a single nucleotide polymorphism base located at the 201st position of each nucleotide sequence of SEQ ID NOs 1 to 96 and all or part of the adjacent sequence thereof. The probe may consist of approximately 5 to 150, 5 to 100, 5 to 50, or 5 to 10 nucleotides, but is not limited thereto. The probe may be manufactured in multiple numbers corresponding to each single nucleotide polymorphism so as to detect each single nucleotide polymorphism by forming DNA-DNA hybridization with a sequence containing each single nucleotide polymorphism base located at the 201st position within each nucleotide sequence, based on each of the nucleotide sequences of SEQ ID NOs 1 to 96. The above probe can be designed and manufactured according to general methods known in the art.

[0050] The term "probe" refers to a polynucleotide or oligonucleotide that specifically binds to a specific target sequence. The probe may be capable of forming a specific binding complementary or specific binding to the polynucleotide or oligonucleotide containing the single nucleotide polymorphism site. The probe may have a different binding affinity depending on the base difference of the single nucleotide polymorphism. The probe may be labeled so that the presence or absence of a specific nucleic acid (a specific type of base) can be confirmed. The polynucleotide or oligonucleotide of the probe may include nucleotide analogs such as phosphorothioates or alkylphosphorothioates, peptide nucleic acids, or intercalations. The polynucleotide or oligonucleotide of the probe may further include a labeling material that emits fluorescence, phosphorescence, or radioactivity. The labeling material may be labeled at the 5' end or 3' end of the polynucleotide or oligonucleotide.

[0051] The above step (b) may include a step of amplifying nucleic acids using the isolated genomic DNA as a template and the above apple variety identification primer set as primers. For example, the above apple variety identification primer set may include all of the above primer sets 1 to 96. The amplification of nucleic acids is performed using PCR, ligase chain reaction, nucleic acid sequence-based amplification, transcription-based amplification system, strand displacement amplification, or Q Amplification can be performed by means of a replicase, or by any other suitable method known in the art for amplifying nucleic acid molecules.

[0052] Step (b) above may include the step of detecting the amplified product through a DNA chip, gel electrophoresis, radioactivity measurement, fluorescence measurement, or phosphorescence measurement. When detecting via gel electrophoresis, agarose gel electrophoresis or acrylamide gel electrophoresis may be used depending on the size of the amplified product. When detecting via fluorescence measurement, PCR may be performed using the primer set labeled with a fluorescent substance, and then the fluorescence may be measured using a fluorescence detector. When detecting via radioactivity measurement, a radioactive substance may be added to the PCR reaction solution to label the amplified product, and then the radioactivity may be measured using a radioactivity detector such as a Geiger counter or a liquid scintillation counter.

[0053] The above step (b) may utilize at least one method selected from the group consisting of polymerase chain reaction (PCR), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), fluorescence melting curve analysis (FMCA), and microarray. The PCR may include allele-specific polymerase chain reaction (AS-PCR), multiplex PCR, or real-time polymerase chain reaction.

[0054] The above PCR may refer to a method of amplifying a target nucleic acid using a polymerase and primers that specifically bind to the target nucleic acid. The above Allele-specific polymerase chain reaction (AS-PCR) is a PCR method for specifically identifying genetic variations in DNA base sequences, and may refer to a method of PCR amplifying a gene region containing a genetic variation using primers or primer sets having specific base sequences for each allele carrying the variation. The above Multiplex PCR may refer to a method of PCR amplifying multiple gene regions simultaneously within a single reaction tube using multiple primer sets. The above Real-time PCR may refer to a method of measuring the amount of fluorescence produced when the fluorescent dye dissociates from the probe during PCR amplification using a probe linked to a specific primer and a fluorescent substance, and may refer to a method in which the amplification of a target DNA molecule and the measurement of its quantity (i.e., detection of the amplification product) can be performed simultaneously. Such PCR methods are well known in the art and may utilize commercially available mix compositions (e.g., PCR reaction solutions) or kits.

[0055] Using the above method, one or more of the apple varieties listed in Table 2 below can be identified. According to one embodiment, using the above method, a general apple variety and a bud mutation variety can be distinguished, and one or more of the apple varieties numbered 1 to 72 in Table 2 below can be identified.

[0056] The above step (c) may include the step of analyzing the detection results of the above step (b) to determine (all) the genotypes of the single nucleotide polymorphism bases located at the 201st base of each base sequence in the base sequences of SEQ ID NOs 1 to 96 in the isolated genomic DNA. Specifically, the step of determining the genotype may be to determine the type of base (i.e., allele) present at all single nucleotide polymorphism positions in Table 3 below (e.g., for single nucleotide polymorphism No. 1 in Table 3 below, determine whether the type of base (i.e., allele) present at the corresponding position is G or A). In addition, the step of determining the genotype may involve determining the genotype of the base pairs present at all single nucleotide polymorphism positions in Table 3 below (i.e., base pairs located at the same locus on homologous chromosomes) (e.g., any one of major allele homozygote, heterozygote, and minor allele homozygote) (e.g., for single nucleotide polymorphism 1 in Table 3 below, determining whether the genotype of the base present at the corresponding position is GG (homozygous), GA (heterozygous), and AA (homozygous)).

[0057] According to one embodiment, the step of determining the genotype may be to analyze the amplification product detected in step (b), that is, the amplification product detected by amplifying nucleic acid using the isolated genomic DNA as a template and the set of primers for identifying apple varieties as primers, and to determine the genotype of a single nucleotide polymorphism located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96 in the isolated genomic DNA. For example, when the isolated genomic DNA is used as a template and the first primer set is used as a primer set for identifying apple varieties to amplify nucleic acids, i) if only the product amplified by a primer that amplifies only the nucleic acid region containing the base at the single nucleotide polymorphism position in SEQ ID NO. 1 is detected, the genotype of the base at the single nucleotide polymorphism position in SEQ ID NO. 1 can be determined to be GG (homozygous), ii) if only the product amplified by a primer that amplifies only the nucleic acid region containing the base at the single nucleotide polymorphism position in SEQ ID NO. 1 is detected, the genotype of the base at the single nucleotide polymorphism position in SEQ ID NO. 1 can be determined to be AA (homozygous), and if both amplification products of i) and ii) are detected, the genotype of the base at the single nucleotide polymorphism position in SEQ ID NO. 1 can be determined to be GA (heterozygous).

[0058] In step (c) above, identifying the apple variety may involve comparing or analyzing the detection result obtained in step (b) above (e.g., amplification product) or the result analyzed from the detection result (e.g., genotype of the single nucleotide polymorphism position base). For example, in step (c), the detection result obtained in step (b) above (e.g., amplification product) or the result analyzed from the detection result (e.g., genotype of the single nucleotide polymorphism position base) may be compared with the result obtained by performing steps (b) and (c) in the same manner on genomic DNA isolated from one or more apple varieties listed in Table 2 below (e.g., amplification product or genotype of the single nucleotide polymorphism position base). For example, by comparing the genotype of the single nucleotide polymorphism position base in the genomic DNA of the apple variety to be identified, obtained by the above method, with the data in Tables 5 to 12 below, which represent the genotype of the single nucleotide polymorphism position base in the genomic DNA of each apple variety listed in Table 2 below, the apple variety to be identified can be determined as a specific apple variety among the apple varieties listed in Table 2 below that matches the genotype of the single nucleotide polymorphism position base.

[0059] Among the terms or elements mentioned in the above method, those mentioned in the description of the primer set or the kit are understood to be the same as those mentioned above in the description of the primer set or the kit. Effects of the invention

[0060] According to the primer set, kit, and method for identifying apple varieties according to one aspect, a large number of apple varieties collected domestically and abroad can be identified with high accuracy at a lower cost and higher efficiency than existing methods by using molecular markers. Therefore, the primer set, kit, and method for identifying apple varieties according to one aspect can be utilized in seed distribution management tasks such as seed variety protection applications and prevention of illegal distribution of seeds, and can also contribute to fields such as verifying the authenticity of apple varieties and mediating seed disputes. Brief explanation of the drawing

[0061] Figure 1 shows the results of analyzing the gene distribution of apple varieties using 96 SNP (single nucleotide polymorphism) markers from Table 3. Figure 2 shows the results of analyzing the genetic polymorphisms of apple varieties using 96 SNP markers from Table 3. Figure 3 schematically illustrates a set of primers for a Fluidigm system for detecting SNP markers (ASP: Allele Specific Primer; LSP: Locus Specific Primer; STA: Specific Target Amplification Primer). Figure 4 is a phylogenetic tree showing the genetic similarity between apple varieties obtained by applying the results of a Fluidigm analysis, which analyzed the genotypes of apple varieties using 96 SNP markers in Table 3 and the first to 96 primer sets in Table 4 that detect them, to UPGMA (Unweighted Pair Group Method with Arithmetic Mean). Specific details for implementing the invention

[0062] The present invention will be explained in more detail below through experimental examples and embodiments. However, these experimental examples and embodiments are intended to illustrate the present invention, and the scope of the present invention is not limited to these experimental examples and embodiments.

[0063] Furthermore, unless otherwise specifically defined in this specification, all scientific and technical terms used herein may have the same meaning as commonly understood by those skilled in the art to which the present invention pertains.

[0065] Example 1: Development of Markers and Primers for Apple Variety Identification

[0066] 1. Selection of Apple Varieties

[0067] The 89 apple varieties listed in Table 2 were used to develop markers and primers for apple variety identification. Specifically, as samples for the analysis of apple gene sequences and the discovery of genetic variations, the 89 apple varieties listed in Table 2 were selected from varieties held by the National Seed Center, the Apple Research Institute of the Rural Development Administration, the National Genetic Resources Center, and the Central Fruit Tree Nursery Center.

[0068] number Sample code Variety name Training institutions division note 1 AP001 RubyS Rural Development Administration Plant Variety Protection General varieties 2 AP004 mountain temple Kim Gyeong-su (Pureun Farm) Production and sales General varieties 3 AP005 Shinano Suite Goryeowon Production and sales General varieties 4 AP006 Shinano Gold An Jung-deok (Hyundai Farm) Production and sales General varieties 5 AP007 Tsugaru Bae Hee-kwon (Gyeongbuk Farm) Production and sales General varieties 6 AP008 Arihwa Rural Development Administration Plant Variety Protection General varieties 7 AP009 Yanggwang Seongrim Farm Production and sales General varieties 8 AP010 EasyPl Rural Development Administration Plant Variety Protection General varieties 9 AP011 topaz Rural Development Administration Plant Variety Protection General varieties 10 AP014 calomel Rural Development Administration Plant Variety Protection General varieties 11 AP015 Green ball Rural Development Administration Plant Variety Protection General varieties 12 AP018 New country Rural Development Administration Plant Variety Protection General varieties 13 AP019 Seo Hong Rural Development Administration Plant Variety Protection General varieties 14 AP020 Crimson Rural Development Administration Plant Variety Protection General varieties 15 AP021 Summer Dream Rural Development Administration Plant Variety Protection General varieties 16 AP022 Summer King Rural Development Administration Plant Variety Protection General varieties 17 AP023 Summer Prince Rural Development Administration Plant Variety Protection General varieties 18 AP024 Arisu Rural Development Administration Plant Variety Protection General varieties 19 AP025 Chugwang Rural Development Administration Plant Variety Protection General varieties 20 AP026 picnic Rural Development Administration Plant Variety Protection General varieties 21 AP027 Hong Jin Rural Development Administration Plant Variety Protection General varieties 22 AP028 Hongro Rural Development Administration Plant Variety Protection General varieties 23 AP029 Hongso Rural Development Administration Plant Variety Protection General varieties 24 AP030 rosy cheeks Rural Development Administration Plant Variety Protection General varieties 25 AP031 Hwahong Rural Development Administration Plant Variety Protection General varieties 26 AP032 Fuji NA Production and sales General varieties 27 AP033 Colorful Rural Development Administration Plant Variety Protection General varieties 28 AP034 Tinkerbell Rural Development Administration Plant Variety Protection General varieties 29 AP035 Silate The New Zealand Institute for Plant and Food Research Plant Variety Protection General varieties 30 AP036 Hwasa (Child Love) Rural Development Administration Plant Variety Protection General varieties 31 AP038 Wonkyo Gadan Gwaji No. 1 Rural Development Administration Plant Variety Protection General varieties 32 AP040 Manbok Rural Development Administration Plant Variety Protection General varieties 33 AP041 Sobaek 3 Park Jun-won (Sobaek Farm) Plant Variety Protection General varieties 34 AP042 Sobaek No. 2 Park Jun-won (Sobaek Farm) Plant Variety Protection General varieties 35 AP045 Golden Bell Rural Development Administration Plant Variety Protection General varieties 36 AP046 Rosebell Rural Development Administration Plant Variety Protection General varieties 37 AP047 Decobell Rural Development Administration Plant Variety Protection General varieties 38 AP048 Jonathan NA Production and sales General varieties 39 AP049 Hongwol NA Production and sales General varieties 40 AP050 World Day NA Production and sales General varieties 41 AP052 Alps Otome NA Production and sales General varieties 42 AP053 Jonagold NA Production and sales General varieties 43 AP054 Aori No. 3 Korean agricultural materials Production and sales General varieties 44 AP055 Cheonchu NA Production and sales General varieties 45 AP056 Big Dipper NA Production and sales General varieties 46 AP057 Shinheung (New Jonagold) NA Production and sales General varieties 47 AP058 Maurice Delicious NA Production and sales General varieties 48 AP059 Mutsu (65) NA Production and sales General varieties 49 AP060 Chuhyang NA Production and sales General varieties 50 AP061 Gukgwang NA Production and sales General varieties 51 AP062 stockings NA Production and sales General varieties 52 AP063 Spur Golden Delicious NA Production and sales General varieties 53 AP064 Summer Champion NA Production and sales General varieties 54 AP065 royalty NA Production and sales General varieties 55 AP066 India NA Production and sales General varieties 56 AP067 July Red NA Production and sales General varieties 57 AP068 Fireside NA Production and sales General varieties 58 AP069 Gala NA USA General varieties 59 AP080 Golden Ball Rural Development Administration Plant Variety Protection General varieties 60 AP083 M.9 NAKBT337 NA uk General varieties 61 AP084 Malling 26 the East Malling Research Station uk General varieties 62 AP085 Budagovsky 9, Bud.9 NA russia General varieties 63 AP086 Malling Merton 111 EMLA, M.1, M.111 the John Innes Horticultural Institute and the East Malling Research Station uk General varieties 64 AP087 Polish 2 the Research Institute of Poland Poland General varieties 65 AP088 Kari2 Rural Development Administration Plant Variety Protection General varieties 66 AP089 Kari1 Rural Development Administration Plant Variety Protection General varieties 67 AP090 Kari3 Rural Development Administration Plant Variety Protection General varieties 68 AP101 Granny Smith Maria Ann Smith australia General varieties 69 AP104 Cox Orange Pippin Richard Cox, Colnbrook in Buckinghamshire, England uk General varieties 70 AP107 In Akiba Takeo Odakiri japan General varieties 71 AP110 Rose Pearl Japan National Research Organization for Agriculture and Food Industry Technology Cultivation screening General varieties 72 AP113 Redfield the New York Agricultural Experiment Station Geneva USA General varieties 73 AP002 Miyama Fuji Park Jun-won (Sobaek Farm) Plant Variety Protection Fujiazo variant 74 AP003 Miyabi Woo Yong-seong (Handeul Farm) Production and sales Fujiazo variant 75 AP012 Fuji Royal Kim Yong-geun (Saejae Farm) Production and sales Fujiazo variant 76 AP013 Fuji Champion Oh Su-seok (Yeongsaeng Farm) Production and sales Fujiazo variant 77 AP016 Nagafuji NA NA Fujiazo variant 78 AP017 Danhong Rural Development Administration Plant Variety Protection Fujiazo variant 79 AP037 Fuji Hubrac KIKU GmbH-srl Plant Variety Protection Fujiazo variant 80 AP039 Hwayoung Rural Development Administration Plant Variety Protection Fujiazo variant 81 AP043 Sairai Park Jun-won (Sobaek Farm) Plant Variety Protection Fujiazo variant 82 AP044 village Kim Jae-young Plant Variety Protection Fujiazo variant 83 AP051 royal household NA Production and sales Fujiazo variant 84 AP095 Jahong Im Sang-cheol Plant Variety Protection Hongroajo variant 85 AP103 Mishima Fuji Samlip Seedling Center, Bugok Farm Production and sales Fujiazo variant 86 AP105 Goun Ihuji Lee Jun-gu Plant Variety Protection Fujiazo variant 87 AP143 Royal King Fuji Oh Jae-young Plant Variety Protection Fujiazo variant 88 AP146 Chohong Lee Seok-gu Plant Variety Protection Hongroajo variant 89 AP149 Hong Dream sliding door Plant Variety Protection Hongroajo variant

[0070] 2. Isolation of apple genome DNA

[0071] Apple genome DNA is NucleoSpin ®The samples were isolated using the Plant Genome Kit (MN Co., Germany). After grinding the apple samples, approximately 300 µl of lysis buffer was added to the ground samples, and the reaction was carried out at approximately 60°C for about 3 hours. After the reaction was complete, the samples were centrifuged at approximately 10,000 xg for about 10 minutes, and approximately 300 µl of the supernatant was collected. Approximately 75 µl of precipitation buffer was added to the approximately 300 µl of supernatant, and the mixture was centrifuged at approximately 10,000 xg for about 5 minutes. Then, approximately 450 µl of binding solution was added and mixed homogeneously, after which the mixture was passed through a column to remove impurities. The column was washed approximately 3 times with approximately 600 µl of wash buffer, and then genomic DNA was recovered from the column using approximately 100 µl of sterile distilled water. The extracted genomic DNA was used for sequencing analysis after measuring its concentration and purity using an ND-1000 UV-Vis spectrophotometer (NanoDrop Technologies, USA).

[0073] 3. DNA Sequencing and Gene Variant Analysis

[0074] To identify single nucleotide polymorphisms (SNPs) for apple variety identification, genotype by sequencing (GBS) analysis was performed on 89 selected apple varieties listed in Table 2 above. Barcode sequences were attached to each sample, and sequence demultiplexing was performed to analyze each nucleotide sequence. Subsequently, the raw sequenced data was trimmed according to sequence quality to obtain clean reads. The clean reads were aligned with a reference genome to construct a consensus sequence, and raw SNPs were extracted.

[0075] Next, an SNP matrix was constructed between the samples, and representative SNPs were selected through an SNP filtering process. Raw SNP positions obtained by aligning and comparing the reads of each sample to a standard genome were selected as candidates, and a list of the union of these candidates was constructed. At this time, non-SNP loci were filled with the conserved sequences of the samples. After constructing the SNP matrix, mis-calling SNPs were filtered by comparing the SNPs between samples, SNPs were classified according to type criteria, and significant SNP candidates were selected.

[0077] 4. Search for Genetic Variations in Apples

[0078] Genomic information obtained through GBS analysis was used to search for SNP markers for apple variety identification. Based on the GBS analysis results, out of a total of 1,196,333 SNPs for the identification of 89 apple varieties, 138,214 SNPs had a minor allele frequency (MAF) of less than approximately 5%, of which 6,840 variety-specific SNPs were identified. Among the identified SNPs, 195 highly reliable SNP markers were selected for use in variety identification, and from these 195 markers, 96 core SNP markers capable of final apple variety identification were selected. For the 89 apple varieties listed in Table 2 above, Fluidigm Juno searching for the aforementioned 96 selected SNP markers TM Genotype analysis based on the system (96 x 96 IFC) was performed.

[0079] Figure 1 shows the results of analyzing the gene distribution of apple varieties using 96 SNP markers in Table 3.

[0080] Figure 2 shows the results of analyzing the genetic polymorphisms of apple varieties using 96 SNP markers from Table 3.

[0081] As a result, as shown in Figures 1 and 2, the 96 selected SNP markers showed clear polymorphism among apple varieties, confirming that they can be used as genetic markers for identifying apple varieties. Specifically, the 96 selected SNP markers clearly showed polymorphism among 72 general apple varieties (numbers 1 to 72 in Table 2), excluding 14 Fuji bud variant varieties and 3 Hongro bud variant varieties among the 89 apple varieties in Table 2, confirming that they can be used as genetic markers for identifying general apple varieties. In addition, since bud variant varieties have very high genetic similarity among varieties, it was confirmed that while it is impossible to distinguish between bud variant varieties using the 96 selected SNP markers, it is possible to identify whether a variety is a bud variant. Through this, it was found that the 96 selected SNP markers can be used as genetic markers to distinguish between general varieties and bud variant varieties. The 96 final selected SNP markers mentioned above are shown in Table 3. For reference, in the sequence list, the bases at each SNP location were listed as W (T or A), R (A or G), Y (C or T), M (A or C), K (G or T), and S (C or G); if the base could not be identified in the sequence of each sequence number, it was listed as N (A, T, C, or G). In the description of SNP locations on the chromosome in Table 3, for example, AG_A002 means that the SNP is located at the 635580th base sequence of apple chromosome 9.

[0082] number SNP marker name Chromosome number Chromosomal SNP location Nucleotide sequence containing SNP (Sequence Number) SNP location in the sequence SNP allele 1 AG_A002 9 635580 1 201st G or A 2 AG_A003 11 35841919 2 201st A or G 3 AG_A004 4 31864364 3 201st G or T 4 AG_A006 2 32942260 4 201st C or T 5 AG_A008 2 10187409 5 201st T or C 6 AG_A009 12 26084884 6 201st A or C 7 AG_A010 8 26213948 7 201st A or T 8 AG_A011 17 34135423 8 201st T or C 9 AG_A012 7 27353114 9 201st A or C 10 AG_A013 12 9532200 10 201st G or A 11 AG_A014 1 14585891 11 201st A or G 12 AG_A015 2 5971896 12 201st G or A 13 AG_A016 17 27535738 13 201st T or C 14 AG_A017 10 1549475 14 201st C or T 15 AG_A019 8 183799 15 201st G or A 16 AG_A020 2 37036743 16 201st C or A 17 AG_A021 12 28199117 17 201st A or C 18 AG_A024 14 23946475 18 201st C or T 19 AG_A026 17 1698376 19 201st C or T 20 AG_A028 15 37061117 20 201st A or G 21 AG_A030 14 12913158 21 201st C or A 22 AG_A033 9 2634519 22 201st C or T 23 AG_A035 16 6502820 23 201st T or A 24 AG_A036 14 1183806 24 201st A or C 25 AG_A037 14 2295418 25 201st G or C 26 AG_A038 12 1582834 26 201st G or A 27 AG_A040 2 19964210 27 201st G or C 28 AG_A042 17 15044953 28 201st A or T 29 AG_A043 8 3407947 29 201st A or C 30 AG_A044 14 28210802 30 201st C or T 31 AG_A046 5 31979517 31 201st C or T 32 AG_A049 15 6278188 32 201st T or A 33 AG_A051 5 41554547 33 201st G or A 34 AG_A052 17 3285906 34 201st G or A 35 AG_A054 0 4911882 35 201st A or G 36 AG_A055 2 13085789 36 201st A or C 37 AG_A056 7 3082060 37 201st T or A 38 AG_A057 9 1806786 38 201st G or A 39 AG_A059 15 27450130 39 201st G or T 40 AG_A061 11 4061182 40 201st T or C 41 AG_A064 12 31524734 41 201st A or G 42 AG_A066 5 31632141 42 201st A or G 43 AG_A071 16 7421492 43 201st A or C 44 AG_A072 15 48504451 44 201st A or T 45 AG_A077 3 5566411 45 201st G or A 46 AG_A078 11 39943258 46 201st C or G 47 AG_A079 6 35899993 47 201st G or T 48 AG_A080 3 6343290 48 201st G or A 49 AG_A081 5 45230302 49 201st G or C 50 AG_A082 0 26737662 50 201st T or C 51 AG_A089 1 23133740 51 201st C or T 52 AG_A095 13 3632150 52 201st A or G 53 AG_A096 15 990620 53 201st G or T 54 AG_A097 4 22452973 54 201st T or C 55 AG_A098 12 1730136 55 201st C or T 56 AG_A100 14 24502875 56 201st A or G 57 AG_A102 4 25450738 57 201st G or T 58 AG_A105 9 32030218 58 201st C or A 59 AG_A106 12 19926501 59 201st C or T 60 AG_A108 10 36900656 60 201st T or C 61 AG_A110 7 36419742 61 201st C or A 62 AG_A118 14 20578710 62 201st A or G 63 AG_A127 4 26882427 63 201st C or A 64 AG_A130 17 31757237 64 201st C or T 65 AG_A131 3 36839047 65 201st C or T 66 AG_A135 4 31407396 66 201st T or C 67 AG_B003 0 2246762 67 201st A or G 68 AG_B004 0 1053797 68 201st C or A 69 AG_B005 1 30397440 69 201st T or A 70 AG_B008 0 16434207 70 201st G or A 71 AG_B016 4 24138373 71 201st C or A 72 AG_B017 0 6565545 72 201st T or A 73 AG_B018 4 27584406 73 201st A or G 74 AG_B019 2 931802 74 201st T or A 75 AG_B020 10 21196414 75 201st C or T 76 AG_B021 1 22277960 76 201st G or A 77 AG_B032 0 11240176 77 201st A or G 78 AG_B037 0 2746830 78 201st C or T 79 AG_B040 1 22356311 79 201st A or G 80 AG_B043 2 9639477 80 201st T or C 81 AG_B058 3 30996531 81 201st A or G 82 AG_B059 11 42053023 82 201st T or C 83 AG_A045 16 24625539 83 201st A or T 84 AG_A069 11 42120053 84 201st A or G 85 AG_A090 13 3317449 85 201st T or A 86 AG_A104 9 6788570 86 201st A or C 87 AG_A109 1 13497019 87 201st A or G 88 AG_A116 4 28071365 88 201st C or G 89 AG_A124 12 29201959 89 201st C or A 90 AG_A133 13 8337948 90 201st C or G 91 AG_B033 0 17833956 91 201st C or G 92 AG_B034 0 1065590 92 201st T or C 93 AG_B046 2 310249 93 201st A or T 94 AG_B052 2 1588806 94 201st T or A 95 AG_B055 0 26730676 95 201st C or T 96 AG_B060 7 22906702 96 201st C or T

[0084] 5. Preparation of Fluidigm Primers for Apple Variety Identification

[0085] Fluidigm Juno to detect the finally selected SNP markers in Table 3 above TM As primers applicable to the system, a set of Fluidigm primers for apple variety identification was prepared.

[0086] Figure 3 schematically illustrates a set of primers for a Fluidigm system for detecting SNP markers (ASP: Allele-Specific Primer; LSP: Locus Specific Primer; STA: Specific Target Amplification Primer).

[0087] As a result, a total of 96 sets of primers for apple variety identification were obtained to detect the finally selected SNP markers of Table 3, and are shown in Table 4. In addition, as shown in Table 4, it was found that each of the primer sets included in the total of 96 sets of primers for apple variety identification obtained detected one specific SNP marker corresponding to one of the 96 SNP markers of Table 3.

[0088] Primer set Detectable targets primer SNP marker name ASP1_Seq (Allele Specific Primer 1) ASP2_Seq (Allele Specific Primer 2) LSP_Seq (Locus Specific Primer) STA_Seq (Specific Target Amplification Primer) 1st Primer Set AG_A002 CTCCATCTGGGGTTGTGAGATAC Sequence No.: 97 CTCCATCTGGGGTTGTGAGATAT Sequence No.: 98 ACAGCGACCTTGCTGCTCT Sequence Number: 99 GTGATTCAGCTGGTCGATGC Sequence Number: 100 2nd Primer Set AG_A003 CATTTGGTTCTACGTAGGAACAACTTTT Sequence No.: 101 CATTTGGTTCTACGTAGGAACAACTTTC Sequence No.: 102 GAGCAAAGTTGTGAGCCCTGT Sequence No.: 103 CAAGTCAAAGGAAAATGCTCTATCC Sequence No.: 104 3rd Primer Set AG_A004 AGAAGCTGATGCCTTACTTATCCAC Sequence No.: 105 AGAAGCTGATGCCTTACTTATCCAA Sequence No.: 106 CAATTCCTCAAGTGGCATGGAAACA Sequence No.: 107 GAGAAGCTGATGCCTTACTTATCC Sequence No.: 108 4th Primer Set AG_A006 ACAAACCCAAGTGCCCATTTC Sequence No.: 109 ACAAACCCAAGTGCCCATTTT Sequence No.: 110 CTGCTGCTGCTGCTGTTGA Sequence No.: 111 GCCGCCGTGTTCTTCA Sequence No.: 112 Primer Set No. 5 AG_A008 CTCGGTTCTTGATCATCCGCTTA Sequence No.: 113 CGGTTCTTGATCATCCGCTTG Sequence No.: 114 TCACAGCAATTCCGGTGGAGA Sequence No.: 115 CCTAGATCGAGCAGCAGACTC Sequence No.: 116 Primer Set No. 6 AG_A009 CCTTGCCATTGCCTAACCATT Sequence No.: 117 CCTTGCCATTGCCTAACCATG Sequence No.: 118 GTTCTGAAGAGAGTTGAGGTAGCTCAAATTA Sequence No.: 119 AACAATGTGTCCATGGCTGT Sequence No.: 120 7th Primer Set AG_A010 TTGTAACTAATTTCAGTGCAGGTTTTGAT Sequence No.: 121 TTGTAACTAATTTCAGTGCAGGTTTTGAA Sequence No.: 122 TGGTGAATGTTACGTATGGTAAATCTGTCTAG Sequence No.: 123 CGACAAAACCCTAACAATCTTGTAACT Sequence No.: 124 No. 8 Primer Set AG_A011 CTCAATGTTACCTGCTTTCATTTCCAA Sequence No.: 125 TCAATGTTACCTGCTTTCATTTCCAG Sequence No.: 126 GCAGCAAGCAAACAAATAGAAGACTCA Sequence No.: 127 GGTCCCTTTTCGGTTATTAAAACTCAA Sequence No.: 128 No. 9 Primer Set AG_A012 AGATGCAAATTTTTTGCCACCGAT Sequence No.: 129 GATGCAAATTTTTTGCCACCGAG Sequence No.: 130 CTCCACACCTTCCCTTGAGAGA Sequence No.: 131 TGCTGCACCTACGTTTAAATTTTCT Sequence No.: 132 Primer Set No. 10 AG_A013 GATGTCCTTCAGTAACCTGGATCTC Sequence No.: 133 GATGTCCTTCAGTAACCTGGATCTT Sequence No.: 134 TCAAATGACTCAAAGAAACAAGACCT Sequence No.: 135 GGGTCTCAATTGCTAAAGAGCAT Sequence No.: 136 Primer Set No. 11 AG_A014 GCTTTTCTCTTTGCTTCCCTGGTA Sequence No.: 137 CTTTTCTCTTTGCTTCCCTGGTG Sequence No.: 138 CCGAAACAAGGTCTGGCATGAGAAATATAT Sequence No.: 139 GAGTGCATGTAGATGCAGCTT Sequence No.: 140 Primer Set No. 12 AG_A015 CCAGCAGCATCCTCAGAAGTAC Sequence No.: 141 CCAGCAGCATCCTCAGAAGTAT Sequence No.: 142 ACTGCATGCCTTGTTCTAACATCCT Sequence No.: 143 CCAGCAGCATCCTCAGAAGT Sequence No.: 144 The 13th Primer Set AG_A016 CCAGACTTAATTCGCTTTGCCTT Sequence No.: 145 CCAGACTTAATTCGCTTTGCCTC Sequence No.: 146 GACCAAAAACAAGCTGCAACAAGC Sequence No.: 147 AGCCATACAAGGATGAATCCAGA Sequence No.: 148 The 14th Primer Set AG_A017 CAGCTCGCTTGGCTTCC Sequence No.: 149 GCAGCTCGCTTGGCTTCT Sequence No.: 150 GGGTTCCTTGCATTTCAAAGACTTGT Sequence No.: 151 CTTACATGGTGGAAGGCCTAG Sequence No.: 152 The 15th Primer Set AG_A019 AAAATACCTTGGATAGAACAGACAAATTTCTATTC Sequence No.: 153 AAAATACCTTGGATAGAACAGACAAATTTCTATTT Sequence No.: 154 TGATGCTCATAACGTTTATTATCGAATTTGAATGATTT Sequence No.: 155 AGGCACTGCAGCTCAAAAAT Sequence No.: 156 Primer Set No. 16 AG_A020 GCGGAACCCAGGATTTGC Sequence No.: 157 GGCGGAACCCAGGATTTGA Sequence No.: 158 CACCGTTTCGACTTCAGGGC Sequence No.: 159 GCAGATGGACAACAATGGCG Sequence No.: 160 The 17th Primer Set AG_A021 AGCTCCGACAGTTGGGTTT Sequence No.: 161 AGCTCCGACAGTTGGGTTG Sequence No.: 162 CGATTGCCTCTGCAGAAGGG Sequence No.: 163 TCGATGATGGTTTTTGTGTCATCAG Sequence No.: 164 No. 18 Primer Set AG_A024 CAAGAAAATGTGTTTGTGTGCTGC Sequence No.: 165 ACAAGAAAATGTGTTTGTGTGCTGT Sequence No.: 166 CCTAATCTCCTGGCCTGACCT Sequence No.: 167 GCGTGCATAAAACAAGAAAATGTGTT Sequence No.: 168 No. 19 Primer Set AG_A026 CAATGTGCTTCCTCTCATTACTTTCG Sequence No.: 169 ACAATGTGCTTCCTCTCATTACTTTCA Sequence No.: 170 ACCACAATTCAATAATCCTAAACCTACAGATCG Sequence No.: 171 TCTCGTGTCTGTACAATGTGCT Sequence No.: 172 No. 20 Primer Set AG_A028 GGATGGAGATTAGTGACCTCACT Sequence No.: 173 GGATGGAGATTAGTGACCTCACC Sequence No.: 174 AACCCAAGATCACTGAGCTCCA Sequence No.: 175 GCTGCTGTTCGCTTATCCG Sequence No.: 176 No. 21 Primer Set AG_A030 CTTGGTTTTTTGTGCTTTTGCTCC Sequence No.: 177 CTTGGTTTTTTGTGCTTTTGCTCA Sequence No.: 178 CCCAATTCACATAACGATTGAAACCCA Sequence No.: 179 GTGAGCTTGGTTTTTTGTGCTT Sequence No.: 180 No. 22 Primer Set AG_A033 GGAGGGAGTTGGCCCAG Sequence No.: 181 GGAGGGAGTTGGCCCAA Sequence No.: 182 CATCGTACATCGGCAGCACC Sequence No.: 183 AGGAGGGAGTTGGCCC Sequence No.: 184 No. 23 Primer Set AG_A035 CGATGGCGGAGATGACGATT Sequence No.: 185 CGATGGCGGAGATGACGATA Sequence No.: 186 CCCACCATTCAAACGGCTCA Sequence No.: 187 CGATGGCGGAGATGACGA Sequence No.: 188 The 24th Primer Set AG_A036 CATGCAGCCACTTTTGTGCA Sequence No.: 189 ATGCAGCCACTTTTGTGCC Sequence No.: 190 ACAAGGACAACCGCACGACA Sequence No.: 191 CATCATGCAGCCACTTTTGTG Sequence No.: 192 No. 25 Primer Set AG_A037 CCAATCCTGCAACAGGATCATTTG Sequence No.: 193 CCAATCCTGCAACAGGATCATTTC Sequence No.: 194 CCACATTCATGGACCAACCCC Sequence No.: 195 CCAATCCTGCAACAGGATCATT Sequence No.: 196 No. 26 Primer Set AG_A038 AGTACCTGTAAGGCACCAACG Sequence No.: 197 AAGTACCTGTAAGGCACCAACA Sequence No.: 198 TGGAAATGCCAACTGGGGAGA Sequence No.: 199 CACGATACAATGGAAGGTAGCAA Sequence No.: 200 No. 27 Primer Set AG_A040 CCACCTCAACCACCAAAATTCC Sequence No.: 201 CCACCTCAACCACCAAAATTCG Sequence No.: 202 CACGGTTGATTCCGCTGCC Sequence No.: 203 ACCACCTCAACCACCAAAATT Sequence No.: 204 No. 28 Primer Set AG_A042 TGTAGGTAAAGTTACAAGCTAAAGCCA Sequence No.: 205 TGTAGGTAAAGTTACAAGCTAAAGCCT Sequence No.: 206 GGCAGCAAACAACTCAAACTGCA Sequence No.: 207 GGGACTGTCTCCGTTGATG Sequence No.: 208 No. 29 Primer Set AG_A043 ACCATCTTCAACAACTATTGGCCT Sequence No.: 209 CCATCTTCAACAACTATTGGCCG Sequence No.: 210 TGCAAGCGAAGCTGCCG Sequence No.: 211 CAGAACCACCATCTTCAACAACTATT Sequence No.: 212 No. 30 Primer Set AG_A044 TCGTATTAAAATGGTTTAGTTGATTGCAGG Sequence No.: 213 CTCGTATTAAAATGGTTTAGTTGATTGCAGA Sequence No.: 214 GTGGATCACTCAGAGTAGAAGGTGC Sequence No.: 215 CCCAGCAGTTTAGATTAGTTTAAAGAAGA Sequence No.: 216 No. 31 Primer Set AG_A046 TGTCCCCAGCGAAGAACAC Sequence No.: 217 TGTCCCCAGCGAAGAACAT Sequence No.: 218 CTAGCTATGCGGATTCGCAGC Sequence No.: 219 GCGCTATATTAGCTCCGGC Sequence No.: 220 No. 32 Primer Set AG_A049 ACACCTTCTGGAAAAGAACCAAGA Sequence No.: 221 ACACCTTCTGGAAAAGAACCAAGT Sequence No.: 222 CGCGTTGCTTGAGCACTTGA Sequence No.: 223 AGGAGAGGTGTTGAACACCTT Sequence No.: 224 No. 33 Primer Set AG_A051 ACCCATCAGGCCATAACACG Sequence No.: 225 AACCCATCAGGCCATAACACA Sequence No.: 226 CGGTCTGTTTAATGTTTTTCATCAATTTCTGC Sequence No.: 227 TGACCACCTGTGATATCCAAAGA Sequence No.: 228 The 34th Primer Set AG_A052 GCAACATCAGCAGGTGTCATATC Sequence No.: 229 GCAACATCAGCAGGTGTCATATT Sequence No.: 230 GGGCGTTTGTTGGGGGAAAC Sequence No.: 231 CATCCTCTACATCAGATTTCGGC Sequence No.: 232 The 35th Primer Set AG_A054 GCCTAGACTATATGCTGGAGCT Sequence No.: 233 GCCTAGACTATATGCTGGAGCC Sequence No.: 234 CTGAGCAACTTGAAGGGCCA Sequence No.: 235 TGGCCTAGACTATATGCTGGAG Sequence No.: 236 No. 36 Primer Set AG_A055 TGGGTCCCTGAGAGTCATCAA Sequence No.: 237 GGGTCCCTGAGAGTCATCAC Sequence No.: 238 CGCCAGCGGTATTGCCT Sequence No.: 239 GGGCTTTTGGTCAATTCACTTG Sequence No.: 240 No. 37 Primer Set AG_A056 CAAATGGAACAAGTGCACCTTTGA Sequence No.: 241 AAATGGAACAAGTGCACCTTTGT Sequence No.: 242 GTGACTCGAAGCCTCTCTTCCATAT Sequence No.: 243 CTAGCCGTGAATGCTTTTGAATC Sequence No.: 244 No. 38 Primer Set AG_A057 CGCAGACGACGAAGCTGAC Sequence No.: 245 CGCAGACGACGAAGCTGAT Sequence No.: 246 GCCGCAATTGAGCGCCA Sequence No.: 247 CGCAGACGACGAAGCTG Sequence No.: 248 No. 39 Primer Set AG_A059 CAGATTAAACAGAGTTATTAGTGTAGTGCC Sequence No.: 249 GCAGATTAAACAGAGTTATTAGTGTAGTGCA Sequence No.: 250 GCAGCATTATGATTCATTGACATGCACTAATTAA Sequence No.: 251 AGGGTGTGTGCCCCTG Sequence No.: 252 No. 40 Primer Set AG_A061 AAAAGCTCGCTTACTTTATATGTTGCAAT Sequence No.: 253 AAGCTCGCTTACTTTATATGTTGCAAC Sequence No.: 254 GGTGGCAGGTTATGCTGTTGA Sequence No.: 255 AGCAGAAAAGCTCGCTTACTTTAT Sequence No.: 256 No. 41 Primer Set AG_A064 GGCTGCCATTGCTGATTCA Sequence No.: 257 GGCTGCCATTGCTGATTCG Sequence No.: 258 TTTTCGCAAATGAGGTTTTGTACCTGAG Sequence No.: 259 CTGGCTGCCATTGCTGATT Sequence No.: 260 No. 42 Primer Set AG_A066 GAGGATTATGTCACGCATTTATGCA Sequence No.: 261 GAGGATTATGTCACGCATTTATGCG Sequence No.: 262 GTGATGGTAAGAATTTCTGATTTTGTGTATCCAA Sequence No.: 263 GGTTGATTTATGACATCAGGCCG Sequence No.: 264 No. 43 Primer Set AG_A071 GGAATTCAAGAGAAGAGGTGTTCTTCT Sequence No.: 265 GAATTCAAGAGAAGAGGTGTTCTTCG Sequence No.: 266 GCTCTCCGAGTTCTCGCAAC Sequence No.: 267 CTAAAATGGCGGGTTTAAATTTGGA Sequence No.: 268 No. 44 Primer Set AG_A072 GAAGAAGTGACGTACCATGTGGTA Sequence No.: 269 AAGAAGTGACGTACCATGTGGTT Sequence No.: 270 ACCTTTCTTCAATGGCGGCAC Sequence No.: 271 ACTACACAAGCATGGATTTGATTGA Sequence No.: 272 No. 45 Primer Set AG_A077 GCCGAAGCTTTGTCGAAGC Sequence No.: 273 AGCCGAAGCTTTGTCGAAGT Sequence No.: 274 CAGTGAGACAGAACTTCTGGCG Sequence No.: 275 AGTGGGTGGAACAAGTTCTCA Sequence No.: 276 No. 46 Primer Set AG_A078 TGACAGGATGGCCGGC Sequence No.: 277 TGACAGGATGGCCGGG Sequence No.: 278 AGCGGTGTTTGTTGCCCC Sequence No.: 279 TGTTTCTTGAGGCAATGGTTGT Sequence No.: 280 No. 47 Primer Set AG_A079 TTTTAGTTATGATTTCTCTGTTGTAAATTTTCAGAG Sequence No.: 281 TTTTAGTTATGATTTCTCTGTTGTAAATTTCAGAT Sequence No.: 282 TCATGTTGGAATGGTAACTATACGAAATGAATTCT Sequence No.: 283 ACCTACGAAAATCCAATCCAACAAT Sequence No.: 284 No. 48 Primer Set AG_A080 GATATGCAGAATGTGTCACAAAGGAAG Sequence No.: 285 GATATGCAGAATGTGTCACAAAGGAAA Sequence No.: 286 TGATCCTGTGAGTTTCTAAATACGTTTACTTTCAT Sequence No.: 287 AAATTTTGACCTGTTCAACATTCAGTTT Sequence No.: 288 No. 49 Primer Set AG_A081 GTTTCCGGCCAAACTTACGC Sequence No.: 289 GTTTCCGGCCAAACTTACGG Sequence No.: 290 TGGCCGCCACCGTGA Sequence No.: 291 AGAACAGAAGACCGCCGAA Sequence No.: 292 No. 50 Primer Set AG_A082 CATACTATGTACTCAACCAACAGAGGA Sequence No.: 293 CATACTATGTACTCAACCAACAGAGGG Sequence No.: 294 GTTGCAAACACGATTTAAGCAAGCAT Sequence No.: 295 CTAATATGCGAAAAGCTGCCAATC Sequence No.: 296 No. 51 Primer Set AG_A089 TCTTATAAACGTGTTTCCCGGACAG Sequence No.: 297 TCTTATAAACGTGTTTCCCGGACAA Sequence No.: 298 GGCAGCAACATCAGTTGCCA Sequence No.: 299 CCGAGTTTTCTCCTCTTATAAACGTG Sequence No.: 300 No. 52 Primer Set AG_A095 TGGGCAGCTGTGATAATTGCT Sequence No.: 301 GGGCAGCTGTGATAATTGCC Sequence No.: 302 ACTGCGAACCATGACGTGAGA Sequence No.: 303 AGTTGGGCAGCTGTGATAATTG Sequence No.: 304 No. 53 Primer Set AG_A096 CTTTAGTGACAAGCATACCAGTCG Sequence No.: 305 GCTTTAGTGACAAGCATACCAGTCT Sequence No.: 306 ACGAAAACTAGCTCAAGATCACATCAGATT Sequence No.: 307 CAAAATAGAAAGTAACTGCTTTAGTGACA Sequence No.: 308 No. 54 Primer Set AG_A097 CCTTCGATGTATGGAAGTACCGT Sequence No.: 309 CCTTCGATGTATGGAAGTACCGC Sequence No.: 310 CAAACCAATACATATACACCCATTTTACCCT Sequence No.: 311 CAGGAAGAATGTAACAAAGGTTGTTC Sequence No.: 312 No. 55 Primer Set AG_A098 TGTGAGCTTCGTATGTGTACATATGTG Sequence No.: 313 ATGTGAGCTTCGTATGTGTACATATGTA Sequence No.: 314 GGCGCACACACACGCAC Sequence No.: 315 CAGCATTTTGCTCCTCCTGTAA Sequence No.: 316 No. 56 Primer Set AG_A100 AGAGAATGTTCTGGCGGAAACT Sequence No.: 317 GAGAATGTTCTGGCGGAAACC Sequence No.: 318 GCTTGGAACTGCAGCGATCA Sequence No.: 319 GAGCTTTGGTGCACCTTGT Sequence No.: 320 No. 57 Primer Set AG_A102 GAGAGATTATTCTTTACCGTCTGGGG Sequence No.: 321 GAGAGATTATTCTTTACCGTCTGGGT Sequence No.: 322 GGTAGCAGCAGCATGATTGCC Sequence No.: 323 ATGAACAACAGAAGTGAGAAAACCA Sequence No.: 324 No. 58 Primer Set AG_A105 TGAGCTAGGCTGCTTAATTAAGTGG Sequence No.: 325 TGAGCTAGGCTGCTTAATTAAGTGT Sequence No.: 326 CCGCCGCCACTCAACAC Sequence No.: 327 ACAATGCCGAAGCCGC Sequence No.: 328 No. 59 Primer Set AG_A106 TCCTGTTGTACGTAGTGGCTTG Sequence No.: 329 CATCCTGTTGTACGTAGTGGCTTA Sequence No.: 330 AGGATAACACTTCGCACCCCA Sequence No.: 331 CCCCGAAAGGAAGTATGCAG Sequence No.: 332 No. 60 Primer Set AG_A108 GGTCACAGAACTCCATGTATTCCAAT Sequence No.: 333 GTCACAGAACTCCATGTATTCCAAC Sequence No.: 334 TGAAGAAAACGGCTGGGGACA Sequence No.: 335 CCTTAAGAGAGGGTTTCAAGTCATG Sequence No.: 336 No. 61 Primer Set AG_A110 AGTAAGTCATTTGTTACACAAATATGATATACTAAC Sequence No.: 337 AGTAAGTCATTTGTTACACAAATATGATATACTAAA Sequence No.: 338 TGTTTCTAATCTGATTATCGATATTCAATCTTGATTCGT Sequence No.: 339 GGAGACTTGATCATCCACCTCA Sequence No.: 340 No. 62 Primer Set AG_A118 GCCTCAGGAATATTCGAGCACA Sequence No.: 341 GCCTCAGGAATATTCGAGCACG Sequence No.: 342 CCTGATTGTGAAGCCCGTGG Sequence No.: 343 AGCCTCAGGAATATTCGAGCA Sequence No.: 344 No. 63 Primer Set AG_A127 CCTGGAGACCATTGCAGCTAC Sequence No.: 345 CCTGGAGACCATTGCAGCTAA Sequence No.: 346 CAGCTCCCATTGCAAGCAGAA Sequence No.: 347 CTGGAGACCATTGCAGCT Sequence No.: 348 No. 64 Primer Set AG_A130 TGTGCACTGAAACATTTACATTCACTG Sequence No.: 349 CATGTGCACTGAAACATTTACATTCACTA Sequence No.: 350 ACGTCCGTGGTATGTTTAGAAGATGC Sequence No.: 351 ATGTGCACTGAAACATTTACATTCAC Sequence No.: 352 No. 65 Primer Set AG_A131 GGACAAGGGTTTGTGGCAG Sequence No.: 353 GGACAAGGGTTTGTGGCAA Sequence No.: 354 GCTTGGTGCTTCCAGGGC Sequence No.: 355 TTGGACAAGGGTTTGTGGC Sequence No.: 356 No. 66 Primer Set AG_A135 CAGACGACGCATAGTCTTGGTTAT Sequence No.: 357 AGACGACGCATAGTCTTGGTTAC Sequence No.: 358 GACACATGGTCTTACCTGCCG Sequence No.: 359 TTTGCCGTCAGCTTTCTTGAA Sequence No.: 360 No. 67 Primer Set AG_B003 GAACAGGTTTTCGACCTCTCTCT Sequence No.: 361 AACAGGTTTTCGACCTCTCTCC Sequence No.: 362 GCGTTTGTGGGGAATCGTCTATGT Sequence No.: 363 ACCTCTCTAAATTTGGATACTCCAAAGA Sequence No.: 364 No. 68 Primer Set AG_B004 GTACTGAACTACAGAATGCACAACG Sequence Number: 365 GGTACTGAACTACAGAATGCACAACT Sequence No.: 366 TGTTATCTATTTTACAATTTGGCTGCAAGTTTTTCT Sequence No.: 367 CTTGAATCAGACCTGTTTCGGT Sequence No.: 368 No. 69 Primer Set AG_B005 CTGCTTCTCCTGTGGGGAAATA Sequence No.: 369 TGCTTCTCCTGTGGGGAAATT Sequence No.: 370 CACTCTCTACCGGCCTTCACT Sequence No.: 371 TGCTTCTCCTGTGGGGAAA Sequence No.: 372 No. 70 Primer Set AG_B008 CTTGAATCGCCTTGAGATTCTCG Sequence No.: 373 CCTTGAATCGCCTTGAGATTCTCA Sequence No.: 374 AGTTCTCTGGAGAGAAGGTTATAGCTCA Sequence No.: 375 TTCTTAAGAACTTAACCATTCTTGTCCTT Sequence No.: 376 No. 71 Primer Set AG_B016 TCCGATTGAATCTAATTAGACCTTGTATGTATG Sequence No.: 377 CTCCGATTGAATCTAATTAGACCTGTATGTATT Sequence No.: 378 CCAAAATGTATAACCATATCAGTAGGAACATACATACA Sequence No.: 379 AAGTGAGTTGTTCTCTTTTCTCCG Sequence No.: 380 No. 72 Primer Set AG_B017 AAACATAAAAAATGTTAAACTCCACAGGACA Sequence No.: 381 AAACATAAAAAATGTTAAACTCCACAGGACT Sequence No.: 382 GTGTTATGTTATCAGCGTTTTGATAATTACACTCT Sequence No.: 383 GCAAGTCACACGAAAAAACAAACATAAA Sequence No.: 384 No. 73 Primer Set AG_B018 CATTTCTGGGCAGCTTTAATTGACTTA Sequence No.: 385 TTTCTGGGCAGCTTTAATTGACTTG Sequence No.: 386 AGGAAATGTGCTGAAAAAAGAGGAA Sequence No.: 387 ATTTCTGGGCAGCTTTAATTGACT Sequence No.: 388 No. 74 Primer Set AG_B019 CTCCTTAAAGTAGCGGAGGCT Sequence No.: 389 CTCCTTAAAGTAGCGGAGGCA Sequence No.: 390 CGTCGAAACTTCGAAAGAATGGCT Sequence No.: 391 CGAGCATATTCTCGCGCT Sequence No.: 392 No. 75 Primer Set AG_B020 TCTCACAAAACCCATCTAGCATACC Sequence No.: 393 TCTCACAAAACCCATCTAGCATACT Sequence No.: 394 AAGCTTGACGCAATGCTGCTT Sequence No.: 395 CTGTCAACATAGCAGAACTCTGT Sequence No.: 396 No. 76 Primer Set AG_B021 GGGGCTCCTAGTGGGAC Sequence No.: 397 AGGGGCTCCTAGTGGGAT Sequence No.: 398 GCGCTCTCCTGACCCTGT Sequence No.: 399 GGGTGCACTTATTCCATCTAAGGA Sequence No.: 400 No. 77 Primer Set AG_B032 CTACTCCACTGTTGGAGGTTAAGT Sequence No.: 401 ACTCCACTGTTGGAGGTTAAGC Sequence No.: 402 GACAAGGCCTGTTGGGCA Sequence No.: 403 ACTACTCCACTGTTGGAGGTTAA Sequence No.: 404 No. 78 Primer Set AG_B037 GTAATGACTCTTGATTAATGACTTTTGTATGTCTC Sequence No.: 405 GTAATGACTCTTGATTAATGACTTTTGTATGTCTT Sequence No.: 406 GCAACAGTTGATACAATCTAATAAGGTTAATCAAGAG Sequence No.: 407 TGATTGCATTTTCTTTAGGGTTTTTCAG Sequence No.: 408 No. 79 Primer Set AG_B040 CTCCCCTCCCCATGACAT Sequence No.: 409 CTCCCCTCCCCATGACAC Sequence No.: 410 CACGAGCTTACAGCAGCAAGG Sequence No.: 411 CCTCCCCTCCCCATGAC Sequence No.: 412 No. 80 Primer Set AG_B043 TTCTTACTGGGTTGACTGTTGCT Sequence No.: 413 TCTTACTGGGTTGACTGTTGCC Sequence No.: 414 CTGTCGCAACTTGGTCGGC Sequence No.: 415 AGACGTATCAAATACAAAATTATGTCACTTCT Sequence No.: 416 No. 81 Primer Set AG_B058 CGTGGCAATGGAATGTTTTAACTCT Sequence No.: 417 CGTGGCAATGGAATGTTTTAACTCC Sequence No.: 418 CTCACCACGATAAACAGTGCAAAGAT Sequence No.: 419 CGGTTTATGTAACATTGGACGTCT Sequence No.: 420 No. 82 Primer Set AG_B059 GAAATCAAGCTTACCTGGAGGAGT Sequence No.: 421 ATCAAGCTTACCTGGAGGAGC Sequence No.: 422 ACTTTGCATGCTTCAAAAGTAACCATTCT Sequence No.: 423 GTCTAGATCGAAATCAAGCTTACCTG Sequence No.: 424 No. 83 Primer Set AG_A045 CAACAAACCAAACCAACATTGAAGAATT Sequence No.: 425 TCAACAAACCAAACCAACATTGAAGAATA Sequence No.: 426 ACAACCACTTTGGAGAGGGACTT Sequence No.: 427 CAACAAACCAAACCAACATTGAAGAA Sequence No.: 428 No. 84 Primer Set AG_A069 CTGCAAACATGTCGAACCTGT Sequence No.: 429 TGCAAACATGTCGAACCTGC Sequence No.: 430 AGTGACGGGAATCTTGTCGCA Sequence No.: 431 GCTGCAAACATGTCGAACCT Sequence No.: 432 No. 85 Primer Set AG_A090 CCAGTACTACTAATGTAGCAACGCATT Sequence No.: 433 CCAGTACTACTAATGTAGCAACGCATA Sequence No.: 434 CGCTACTGTGAGCTCGAGTATGG Sequence No.: 435 CAGCAGCAGCTCTTGATCG Sequence No.: 436 No. 86 Primer Set AG_A104 AATTCTAGCGGAGAACAATGGGT Sequence No.: 437 TTCTAGCGGAGAACAATGGGG Sequence No.: 438 CCAAGTAATCTGATTTCGGTCCCCC Sequence No.: 439 AGAGAGTTAGGTTATTTCAAGCCGA Sequence No.: 440 No. 87 Primer Set AG_A109 GTGCTAGCTCCCCCACTTT Sequence No.: 441 GTGCTAGCTCCCCCACTTC Sequence No.: 442 AGATGCTCTGGTCAGCCGT Sequence No.: 443 GGCATCTCCTGCAAGTAATTGG Sequence No.: 444 No. 88 Primer Set AG_A116 CCACCAAACCACCAAGCG Sequence No.: 445 CCACCAAACCACCAAGCC Sequence No.: 446 GCACCAGCCAAGAAGGTGA Sequence No.: 447 CCCCACCGCCGCTAT Sequence No.: 448 No. 89 Primer Set AG_A124 CCTCATTCACTTTCACAGCATTAACC Sequence No.: 449 CCTCATTCACTTTCACAGCATTAACA Sequence No.: 450 GGTGGGAGTAAGACTCCCGA Sequence No.: 451 CGGATTTCGCCGGTGC Sequence No.: 452 No. 90 Primer Set AG_A133 CCGAAACTTGATCTTTTGTTCAAATTTACG Sequence No.: 453 CCGAAACTTGATCTTTTGTTCAAATTTACC Sequence No.: 454 TCCTTTACAAAACCCATTACCAAAATCCG Sequence No.: 455 CTCCGAAACTTGATCTTTTGTTCAAATTTA Sequence No.: 456 No. 91 Primer Set AG_B033 TGTCCTTCTAGTCCTCCTCACTC Sequence No.: 457 TGTCCTTCTAGTCCTCCTCACTG Sequence No.: 458 TCCAGTCCCTTCTTTTTTATCCCAACTA Sequence No.: 459 CTCGAGTTGTAGTTTTGTCCTTCT Sequence No.: 460 No. 92 Primer Set AG_B034 GATAGAGGCTGGGGCTGTTA Sequence No.: 461 AGAGGCTGGGGCTGTTG Sequence No.: 462 CAGCAACCCCTTCGTTTCCC Sequence No.: 463 GATAGAGGCTGGGGCTGT Sequence No.: 464 No. 93 Primer Set AG_B046 GAAAGCAGTCATATCCTTTCTCTCCATA Sequence No.: 465 AAGCAGTCATATCCTTTCTCTCCATT Sequence No.: 466 TTCAACAAGTTCAGAACAAAGGCACA Sequence No.: 467 TCGTATTACAGATTTGCTAGGGTGA Sequence No.: 468 No. 94 Primer Set AG_B052 GCTGAGAAGCTGAGATTTAAGTTTAGAGTAA Sequence No.: 469 GCTGAGAAGCTGAGATTTAAGTTTAGAGTAT Sequence No.: 470 CCCTCCTCGACGCCGC Sequence No.: 471 CTGAGAAGCTGAGATTTAAGTTTAGAGT Sequence No.: 472 No. 95 Primer Set AG_B055 CTGCTGTTGTCTTGTTTCCTGTTATG Sequence No.: 473 TCTGCTGTTGTCTTGTTTCCTGTTATA Sequence No.: 474 GCACAAAGCCGAAAGCAGAGA Sequence No.: 475 CTGCTGTTGTCTTGTTTCCTGTTA Sequence No.: 476 No. 96 Primer Set AG_B060 CTCGTTTCACAAGACCCTATAGTATCG Sequence No.: 477 CTCGTTTCACAAGACCCTATAGTATCA Sequence No.: 478 TGCCTTCTGATCGCTTGGACT Sequence No.: 479 CGTTGAGAAGTAAATCGGGTTTCT Sequence No.: 480

[0090] 6. Apple variety identification using the Fluidigm system

[0091] Fluidigm-based genotyping and genetic similarity analysis were performed on 89 apple varieties using the primer set of Table 4 prepared to detect the SNP markers of Table 3. The 89 apple varieties used for the Fluidigm analysis are shown in Table 2.

[0092] Specifically, the Fluidigm analysis was performed by using the genomic DNA isolated from each of the 89 apple varieties in Table 2 as a template and conducting KASP (Kompetitive Allele-Specific PCR) using all of the 1st to 96th primer sets in Table 4, which are Fluidigm primer sets, as primers, and then detecting and analyzing the amplified products to determine the allelic genotypes at each position of the 96 SNPs in Table 3 represented by each apple variety. In addition, the genotype results determined for each apple variety were applied to UPGMA (Unweighted Pair Group Method with Arithmetic Mean) to analyze the genetic similarity between apple varieties. The specific methods for the Fluidigm-based genotype analysis using KASP and the genetic similarity analysis using UPGMA as described above were performed according to general methods known in the art. Figure 4 is a phylogenetic tree showing the genetic similarity between apple varieties obtained by applying the results of a Fluidigm analysis, which analyzed the genotypes of apple varieties using 96 SNP markers in Table 3 and the first to 96 primer sets in Table 4 that detect them, to UPGMA (Unweighted Pair Group Method with Arithmetic Mean).

[0093] As a result, as shown in Tables 5 to 12 and Figure 4, Fluidigm analysis was performed using the 1st to 96th primer sets of Table 4 to detect the 96 SNP markers of Table 3. It was confirmed that 100% of the 72 general apple varieties (Nos. 1 to 72 of Table 2) among the 89 apple varieties of Table 2 could be identified, excluding 14 Fuji bud variant varieties and 3 Hongro bud variant varieties. It was also confirmed that while identification between bud variant varieties was impossible, it was possible to identify whether a variety was a bud variant. In Tables 5 to 12, for varieties exhibiting the Homo genotype, the bases at the SNP positions were listed as AA, TT, etc., and for varieties exhibiting the Hetero genotype, they were listed as AG, CT, etc.

[0094] Through this embodiment, it was found that when using the 96 SNP markers of Table 3 and the 1st to 96th primer sets of Table 4 that detect them, it is possible to distinguish between normal apple varieties and bud mutation varieties and accurately identify a large number of normal apple varieties, thereby enabling the identification of a large number of apple varieties at a lower cost and higher efficiency than existing methods.

[0095] Variety / SNP AG_A002 AG_A003 AG_A004 AG_A006 AG_A008 AG_A009 AG_A010 AG_A011 AG_A012 AG_A013 AG_A014 AG_A015 allele G / A A / G G / T C / T T / C A / C A / T T / C A / C G / A A / G G / A AP001 GG AA GT CC TT AA AT TC AA GG AA AA AP002 GA AG GT CT TT AA AT TC AC GG AG AA AP003 GA AG GT CT TT AA AT TC AC GG AG AA AP004 GA AA GT CC TT AA AT TC AA GG AA GG AP005 GG AA GT CC TT AA AA TC AA GA GG AA AP006 GG AA GG CC TT AA AA TC AA GG AG GG AP007 GG AA GG CC TT AA AT TC AC GA AG AA AP008 GG AA GG CC TC AA AA TC AA GG AA GG AP009 GG AA GG CC TT AA AT TC AC GG AG GG AP010 GA AA GG CC TC AA AT TC AA GA AA GA AP011 GA AA GT CT TC AA AT TC AC GG AA GA AP012 GA AG GT CT TT AA AT TC AC GG AG AA AP013 GA AG GT CT TT AA AT TC AC GG AG AA AP014 GA AA GT CC TT AA AT TC AA GA AA AA AP015 GG AA GG CC TC AA AA TC AC GA AG GA AP016 GA AG GT CT TT AA AT TC AC GG AG AA AP017 GA AG GT CT TT AA AT TC AC GG AG AA AP018 GG AA GT CC TT AA AA TC AA GA AG AA AP019 GG AA GT CC TT AA AA TC AA GG AG GG AP020 GA AA GG CC TC AA AT TC AA GA AA GG AP021 GA AA GG CC TT AC AA TC AC GG AA GG AP022 GG AA GG CC TT AA AT TC AA GA AA GG AP023 GG AA GG CC TT AA AT TC AC GG AA AA AP024 GG AG GG CC TT AA AT TC AA GG AG GG AP025 GA AA GT CC TT AA AA TC AA GG AA GG AP026 GG AA GG CC TC AA AA TC AC GA AG GA AP027 GG AA GG CC TT AA AT TC AA GG AG GG AP028 GG AA GG CC TC AA AT TC AA GA AA GG AP029 GG AA GG CC TC AA AT TC AC GG AG GG AP030 GG AG GG CC TC AA AA TC AC GA AA GA AP031 GG AG GG CC TC AA AA TC AC GG AA GG AP032 GA AG GT CT TT AA AT TC AC GG AG AA AP033 GG AG GT CC TT AA AT TC AA GG AA GG AP034 GG AA GT CC TT AA AA TC AA GG AA GG AP035 GA AA GG CT TT AA AT TC AA GG AA GG AP036 GA AA GT CC TT AA AA TC AC GG AG GG AP037 GA AG GT CT TT AA AT TC AC GG AG AA AP038 GG AA GT CC TC AA AT TC AA AA AA GA AP039 GA AG GT CT TT AA AT TC AC GG AG AA AP040 GA AA GT CC TT AA AT TC AA GG AA AA AP041 GG AA GG CC TT AA AT CC AA GA AG AA AP042 GG AA GG CC TT AA AT TC AC GG AG AA AP043 GA AG GT CT TT AA AT TC AC GG AG AA AP044 GA AG GT CT TT AA AT TC AC GG AG AA AP045 GG AA GT CC TT AA AA TC AA GG AA GG AP046 GG AA GT CT TT AA AT TC AA GG AA GG AP047 GA AA GT CC TT AA AT TC AA GG AG GG AP048 GG AA GT CC TT AA AT TC AC GG AG AA AP049 GG AA GT CC TC AA AT TC AC GA AA GG AP050 GA AG GG CC TC AA AA TC AC GA AA GG AP051 GA AG GT CT TT AA AT TC AC GG AG AA AP052 GG AA GT CC TT AA AT TC AA GG AA AA AP053 GG AA GT CC TC AA AT TC AC GA AG GA AP054 GG AA GG CC TC AA AA TC AC GA AA GG AP055 GA AG GT CC TT AA AT TC AA GG AG GG AP056 GA AG GT CT TT AC AT TC AC GG AG AA AP057 GG AA GT CC TT AA AT TC AC GG AA GG AP058 GG AA GG CC TT AA AT TC AC GG AA GG AP059 GG AA GG CT TC AC AA TC AC GA AA GG AP060 GG AA GG CC TT AA AT TC AC GA AG AA AP061 GG AG GT CC TT AC AT TC AC GG AG AA AP062 GA AG GG CT TT CC AT TC AC GA AA GG AP063 GG AA GG CC TC AA AA TC AC GA AA GG AP064 GG AA GG CC TT CC AT TC AC GG AA GG AP065 GG AA GG CT TC AC AA TC AA GA AA GG AP066 GG AA GT CT TC AC AA CC AC GG AA GG AP067 GA AA GT CC TT AA AT TC AC GG AA GG AP068 GA AA GT CC TC AA AT TC AA GG AG GA AP069 GG AA GT CC TT AA AA TC AA GG AA GG AP080 GG AA GG CC TT AA AA TC AA GG AA GG AP083 AA AG GG CC TT CC AT TC AC GG AG GG AP084 GA AA GG CC TT AA AT TC CC GA AG GG AP085 AA AA GG CC TT AC AT TC CC GG AA GG AP086 GG AA GG CC TT AA AT TC AA GG AA GG AP087 GA AA GG CT TC CC AA TC AC GA AG GG AP088 GG AA GG CC TT AA AT TC AC GG AG GG AP089 GG AA GG CC TT AC AT TC AC GG GG AA AP090 AA AG GG CC TT CC AT TC AC GG AG GG AP095 GG AA GG CC TC AA AT TC AA GA AA GG AP101 GG AA GG CC TT AA AT TC AA GG AA GG AP103 GA AG GT CT TT AA AT TC AC GG AG AA AP104 GA AA GT CC TT AA AT TC AC GG AG GG AP105 THOUGH AG GT CT TT SECOND AT TC AC GG AG SECOND AP107 GG SECOND GG CC TT SECOND SECOND TC SECOND THOUGH AG SECOND AP110 GG SECOND GT TT TT SECOND AT TC AC GG AG GG AP113 THOUGH SECOND GG CC TT AC AT TC SECOND GG SECOND GG AP143 THOUGH AG GT CT TT SECOND AT TC AC GG AG SECOND AP146 GG SECOND GG CC TC SECOND AT TC SECOND THOUGH SECOND GG AP149 GG SECOND GG CC TC SECOND AT TC SECOND THOUGH SECOND GG

[0096] 품종 / SNP AG_A016 AG_A017 AG_A019 AG_A020 AG_A021 AG_A024 AG_A026 AG_A028 AG_A030 AG_A033 AG_A035 AG_A036 allele T / C C / T THOUGH C / A A / C C / T C / T A / G C / A C / T ARE A / C AP001 TT CC GG CC AC CC CT SECOND CA CT TT SECOND AP002 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP003 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP004 TT CC GG CC AC CC CC SECOND CC CT TT SECOND AP005 TT CT THOUGH CC SECOND CT TT AG CC CT TT SECOND AP006 TC CT THOUGH CA AC CC CC AG CC CT TT CC AP007 TC CT GG CC AC CC CT SECOND CA CT TT SECOND AP008 TT CT GG CC SECOND CC CC AG CA CC ARE SECOND AP009 TC CT GG CA CC CC CT SECOND CA CT TT AC AP010 TT CT GG CA AC CC CC AG CC CC ARE SECOND AP011 CC CC GG CC SECOND CC CC AG CC CC TT SECOND AP012 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP013 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP014 TT CT GG CC AC CC CC AG CC CC SECOND AC AP015 CC CC GG CC AC CC CC SECOND CC CT TT AC AP016 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP017 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP018 CC CC GG CC AC CC CC SECOND CC CT ARE SECOND AP019 CC CT GG CC AC CC TT AG CA CC TT SECOND AP020 TT CC GG CA AC CC CT SECOND CA CC TT SECOND AP021 TT CT GG CA AC CC CT SECOND CA CC TT SECOND AP022 TC CT THOUGH CC SECOND CC CC SECOND CC CC ARE SECOND AP023 TT CT THOUGH CC AC CC CT SECOND CC CT TT AA AP024 TC CT GG CA AC CC CC AG CC TT TA AC AP025 CC CC GG CC AC CC CT AG CC CC TT AA AP026 CC CC GG CC AC CC CC AA CC CT TT AC AP027 TT CT GG CC AA CT CC AG CC CT TT AA AP028 TT CT GG CA AC CC CC AA CA CC TA AA AP029 TC CT GG CC AC CC CT AA CA CC TT AC AP030 TC CT GG CC AA CT CC AG CC CT TT AA AP031 TC CC GG CA AC CC CC AG CC CC TT AA AP032 TT CC GA CC AA CT CC AG CC CC TA AA AP033 TT CC GG CC AC CC CC AG CC CT TA AC AP034 TT CC GG CC AA CC CT AA CC CC TA AA AP035 TT CC GG CC AC CC CC AG CC CT TT AA AP036 TT CC GG CC AA CC TT AA CC CT TT AA AP037 TT CC GA CC AA CT CC AG CC CC TA AA AP038 CC CT GG CC AC CC CC AA CC CT TT AA AP039 TT CC GA CC AA CT CC AG CC CC TA AA AP040 TT CC GG CC AA CT CC AG CA CT TA AA AP041 TT CC GG CC AA CC CC AA CC CC TT AA AP042 TT CC GG CC AA CC CC AA CA CC TT AA AP043 TT CC GA CC AA CT CC AG CC CC TA AA AP044 TT CC GA CC AA CT CC AG CC CC TA AA AP045 TT CC GG CC AA CC CC AA CC CT TT AA AP046 CC CC GG CC AA CC CC AA CC CC TT AA AP047 TT CC GG CC AC CC CC AA CC CT TT AA AP048 TT CC GG CC AC CC CT AA AA CC TT AA AP049 TC CT GG CC AA CC CC AA CA CT TT AA AP050 CC CT GG CC AA CC CC AG CC CT TA AC AP051 TT CC GA CC AA CT CC AG CC CC TA AA AP052 TT CC GG CC AA CC TT AG CA CC TT AA AP053 TC CT GG CA AC CC CC AA CA CT TT AC AP054 TC CT GG CA AC CC CC AA CC CT TT AC AP055 TT CC GA CC AA TT CC AG CC CT TA AC AP056 TT CC GA CC AA CT CC AG CC CT TA AA AP057 TT CC GG CC AA CC CC AA CC CT TT AA AP058 TC CC GG CA AC CC CT SECOND CC CC TT SECOND AP059 TC CT GG CA AC CT CC SECOND CC CT TT AC AP060 TC CT GG CC AC CC CT SECOND CA CT TT SECOND AP061 TT CC GG CC SECOND CT CC SECOND CC CT ARE SECOND AP062 TT CC THOUGH CC SECOND CC CC AG CC CT ARE SECOND AP063 TC CT GG CA AC CC CC SECOND CC CT TT AC AP064 TT CT THOUGH CC SECOND CC CC SECOND CC CT TT SECOND AP065 TT CC THOUGH CC AC CC CC AG CC CT ARE AC AP066 TT CC GG CC SECOND TT CC AG CC CT TT SECOND AP067 TC CC GG CC AC CC CC SECOND CA CT ARE SECOND AP068 TC CC GG CC SECOND CC CC SECOND CA CT TT SECOND AP069 TT CC GG CC AC CC CC SECOND CC CT TT SECOND AP080 TT CT THOUGH CC AC CC CC SECOND SECOND CT TT SECOND AP083 TC CC THOUGH CA SECOND CC CC SECOND CA CC TT AC AP084 TC CC THOUGH CA SECOND CC CC SECOND CC CT ARE AC AP085 CC CC GG CC SECOND CC CC SECOND CC CC TT SECOND AP086 CC CC GG CA SECOND CC CC GG CC CC ARE AC AP087 TC CC SECOND CA SECOND CC CC SECOND CC CC TT AC AP088 TT CC GG CC SECOND CT CC SECOND CC TT TT SECOND AP089 TT CC GG CC SECOND CC CC SECOND CA CT TT SECOND AP090 CC CC THOUGH CC SECOND CC CC SECOND CC CC TT AC AP095 TT CT GG CA AC CC CC SECOND CA CC ARE SECOND AP101 CC CC SECOND CC SECOND CC CC SECOND CC CC TT SECOND AP103 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP104 TT CT THOUGH CC SECOND CC CT SECOND CA CT TT SECOND AP105 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP107 TC CC GG CC SECOND CC CC AG CC CT TT SECOND AP110 TC CC THOUGH CC SECOND TT CC SECOND CC CC TT SECOND AP113 TC CC GG CA SECOND CC CC SECOND CA CC TT SECOND AP143 TT CC THOUGH CC SECOND CT CC AG CC CC ARE SECOND AP146 TT CT GG CA AC CC CC SECOND CA CC ARE SECOND AP149 TT CT GG CA AC CC CC SECOND CA CC ARE SECOND

[0097] 품종 / SNP AG_A037 AG_A038 AG_A040 AG_A042 AG_A043 AG_A044 AG_A046 AG_A049 AG_A051 AG_A052 AG_A054 AG_A055 allele G / C G / A G / C A / T A / C C / T C / T T / A G / A G / A A / G A / C AP001 GG GA GG AT AA CC TT TT GA GA AA AC AP002 GC GG GG AA AC CC CT TT GG GG GG AC AP003 GC GG GG AA AC CC CT TT GG GG GG AC AP004 GG GA GG AA AA CC CT TT GA GA AA AC AP005 GG GA GG AA AC CC TT TT GG GA AA AA AP006 CC GG GG AA AC CC CC TA GA GG AA AA AP007 GG GA GG AA AC CC TT TA GG GA AA AA AP008 GG GG GG AA AA CC TT TT GA GG AA AA AP009 GC GA GG AA AC CC CT TA GA GA AA AA AP010 GG GG GG AA AC CC CC TT GA GA AA AA AP011 GG GG GG AA AC CC TT TA GG GG AA AA AP012 GC GG GG AA AC CC CT TT GG GG GG AC AP013 GC GG GG AA AC CC CT TT GG GG GG AC AP014 GC GG GG AA AC CC TT TT GG GA AA AA AP015 GC GG GG AA AC CT TT TT GG GG - AA AP016 GC GG GG AA AC CC CT TT GG GG GG AC AP017 GC GG GG AA AC CC CT TT GG GG GG AC AP018 GG GG CC AA AC CC CC TA GA GG AA AA AP019 GC GA GG AA AC CC TT TA GG GA AA AA AP020 GC GG GG AA AA CC CT TT GG GG AA AA AP021 GC GA GG AA AC CC TT TT GG GA AA AA AP022 GC AA GC AA AC CC CT TA GG GG AA AA AP023 GC GA GC AA AC CC CT TT GG GA - AA AP024 GC GA GG AA AC CC CT TT GA GG AA AA AP025 GC GG GG AA AC CC TT TT GG GG AA AC AP026 GC GG GG AA AC CT TT TT GG GG - AA AP027 GG GG GG AT AC CC CT TT AA GG - AA AP028 GG GG GG AA AC CC CT TT GA GG AA AA AP029 GC GG GG AA AC CC CC TT GG GA AA AA AP030 GC GG GG AA AC CC CT TA GG GG GG AA AP031 GG GG GG AT AC CT CT TT GG GG - AC AP032 GC GG GG AA AC CC CT TT GG GG GG AC AP033 GC GA GG AA AC CC CT TA GA GG AA AC AP034 GG GG GG AA AC CT CT TA GA GG AG AC AP035 GC GG GC AA AC CC CC TT GG GG - AA AP036 GC GA GG AA AC CC TT TA GG GA GG AC AP037 GC GG GG AA AC CC CT TT GG GG GG AC AP038 GC GG GG AA AC CT CC TT GG GA AA AA AP039 GC GG GG AA AC CC CT TT GG GG GG AC AP040 GC GG GG AA AC CC CT TT GG GG AA AA AP041 GG GA GG AT AA CC CT TT GA GG AA AA AP042 GG GA GG AT AA CC CT TT GA GG AA AA AP043 GC GG GG AA AC CC CT TT GG GG GG AC AP044 GC GG GG AA AC CC CT TT GG GG GG AC AP045 GG GG GC AA AA CC CC TT GA GG AA AC AP046 GG GG GG AA AC CC CT TT GG GG AA AA AP047 GG GA GG AA AA CC CT TT GA GA AA AA AP048 GG GA GG AT AC CC TT TT GA GA AA AA AP049 GG GG GG AT AC CC CT TA GG GG AA AA AP050 GC GG GG AA AC CC TT TA GG GA - AA AP051 GC GG GG AA AC CC CT TT GG GG GG AC AP052 GG GA GG AT AC CC TT TT GA GA AA AA AP053 GC GG GG AT AC CT CT TA GA GA AA AA AP054 GC GG GG AA AC CT CT TA GG GA AA AA AP055 GC GG GG AT AC CC CT TA GA GG AA AA AP056 GC GG GG AT AC CC CT TT GG GG AG AC AP057 GG GG GG AA AC CC CT TT GA GG AG AA AP058 GC GG CC AA AC CC TT TT GG GA AA AA AP059 GC GG GG AT AC CT CT TA GA GA AA AC AP060 GG GA GG AA AC CC TT TA GG GA AA AA AP061 GC GG GG AA AC CC CT TT GG GG AG AA AP062 GC GG GG AA AC CC CT TT GG GG - AC AP063 GC GG GG AA AC CT CT TA GG GA AA AA AP064 GC GG GG AA AC CC TT TT GG GG GG AA AP065 GC GG GG AA AC CT CT TA GG GG AA AC AP066 GC GG GG AT CC CC CT TT GA GG AA AC AP067 GC GG GG AA AC CC CT TT GA GA AG AC AP068 GC GA GC AA AC CC CT TT GG GG AA AA AP069 GG GG GC AA AC CC CC TA GA GG AA AC AP080 GG GG GG AA AC CC CT TT GG GG AA AA AP083 GC GG GG AA AC CT CT TT GG AA AA AA AP084 GC GG GG AA AC CC TT TT GG GA - AA AP085 GG GG GG AA AA CC TT TT GA GA - AA AP086 GC GG GG AT AA CT CC TT GG GG - AA AP087 GC GG GG AA AC CC CT TT GG GA AA AA AP088 GC GG GG AA AA CC TT TT GG GA AA AA AP089 GG GG GG AA AC CT CT TT GG GG AA AA AP090 GC GG GG AA AA CT CT TT GG AA AA AA AP095 GG GG GG AA AC CC CT TT GA GG AA AA AP101 GC GG GG AA AC CC CT TA GA GA AA AA AP103 GC GG GG AA AC CC CT TT GG GG GG AC AP104 GG GG CC AA AC CC CT TT AA GA AA AA AP105 GC GG GG AA AC CC CT TT GG GG GG AC AP107 GG GA GG AA AC CC CT TT GA GG AA AA AP110 GC GG GG AA AC CC CT TT GG GG GG AC AP113 GG GG GG AA AC CC CT TT GG GA GG AA AP143 GC GG GG AA AC CC CT TT GG GG GG AC AP146 GG GG GG AA AC CC CT TT GA GG AA AA AP149 GG GG GG AA AC CC CT TT GA GG AA AA

[0098] 품종 / SNP AG_A056 AG_A057 AG_A059 AG_A061 AG_A064 AG_A066 AG_A071 AG_A072 AG_A077 AG_A078 AG_A079 AG_A080 allele T / A G / A G / T T / C A / G A / G A / C A / T G / A C / G G / T G / A AP001 TT GG GG TT AA AA AA AT GG CC GG GG AP002 TA GA GT TT AA AG AA AT GA CC GG GG AP003 TA GA GT TT AA AG AA AT GA CC GG GG AP004 TT GG GG TT AA AG AC AT GG CC GG GG AP005 TA GA GG TT AG AA AA AA GG CC GG GG AP006 TT GG GG TT AA AG AA AT GA CC GG GG AP007 TT GG GG TT AG AA AA AA GG CC GG GG AP008 TT GG GG TC AA AA AA AA GG CG GT GA AP009 TA AA GG TT AA AA AA AA WON'T CC GG GG AP010 TT GG GG TC AA AG AA AA WON'T GG TT GG AP011 HIT GG GT TT AA AA AA AA GG CC GG GG AP012 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP013 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP014 TT GG GG TC AA AG AA AA GG CG TT GG AP015 TT GG GG TT AA AA AA AA GG CC GG GG AP016 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP017 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP018 TT GG GG TT AA AG AA AA WON'T CG GG GG AP019 TT GG GG TT AG AA AC AA WON'T CC GG GG AP020 TT GG GT TT AA AA AC AA GG CC GT WON'T AP021 TT GG GT TT GG AA AC AA WON'T CC GG GG AP022 TT WON'T GG TT AG AG AA AA GG CC TT GG AP023 TT WON'T GG TT AA AG AA AA GG CC TT GG AP024 TT GG GG TT AG AA AA AT WON'T CC GG GG AP025 TT GG GT TT AA AA AC AA WON'T CC GG GG AP026 TT GG GG TT AA AA AA AA GG CC GG GG AP027 TT GG GG TC AG AG AA AT WON'T CC GT WON'T AP028 TT GG GG TC AA AA AA AA WON'T CG GT WON'T AP029 HIT WON'T GG TT AA AA AA AA GG CG GG WON'T AP030 TT WON'T GT TC AG AG AA AT GG CC GG GG AP031 TT GG GG TC AA AA AC AA WON'T CC GG GG AP032 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP033 AA AA GG TT AA AG AA AA WON'T CC GG GG AP034 TT AA GG TT AA AG AA AA GG CC GG GG AP035 TT AA GT TC AA AA AA AT GG CC GG GG AP036 TT GG GT TT AG AA AA AT GG CC GG GG AP037 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP038 TT GG GG TC AG AG AA AA GG CC GG WON'T AP039 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP040 TT GG GG TT AA GG AA AA WON'T CG GG GG AP041 TT GG GG TT AA AA AC AA GG CC GG GG AP042 TT GG GG TC AA AA AA AA GG CC GG GG AP043 HIT WON'T GT TT AA AG AA AT WON'T CC GG GG AP044 TA GA GT TT AA AG AA AT GA CC GG GG AP045 TT GG GG TC AA AG AA AT GG CC GG GG AP046 TT GG GG TC AA AA AA AA GG CC GG GG AP047 TT GG GG TT AA AG AA AT GA CC GG GG AP048 TT GA GG TT AA AA AA AA GG CC GG GG AP049 TT GG GG TT AG AA AA AA GA CC GG GG AP050 TA GG GG CC AG AA AA AA GG CC GT GG AP051 TA GA GT TT AA AG AA AT GA CC GG GG AP052 TT GG GT TT AA AA AA AA GG CC GG GG AP053 TA GG GG TC AG AA AA AA GA CC GG GG AP054 TA GG GG TC AG AA AA AA GA CC GG GG AP055 TA GG GG TT AG AG AA AT AA CC GG GG AP056 TA GA GT TT AG AG AA AT GA CC GG GG AP057 TT GG GT TT AA AA AA AT GG CC GG GG AP058 TA GG GT TT AA AA CC AA GA CC GG GG AP059 TA GG GG TC AG AG AA AA GA CC GG GG AP060 TT GG GG TT AG AA AA AA GG CC GG GG AP061 TT GA GT TT AA AA AA AT GG CC GG GG AP062 TA GG GG TC AA AG AC AA GA CC TT GG AP063 TA GG GG TC AG AA AA AA GA CC GG GG AP064 TT GG GG TT AA AA AA AT GA CC GG GG AP065 TA GG GG TC AA AA AA AA GA CC GT GG AP066 TA GG GG TT AA AG AA AA GA CC GG GG AP067 TT GG GG TT AA AG AA AA GG CG GG GG AP068 TT GG GG TC AA AA AA AT GG CC GG GA AP069 TT GA GG TT AA AG AC AT GG CC GG GG AP080 TT AA GG TT AA AA AA AA GG CG GG GA AP083 TT GG GT TT AA AA AA AA GG CC GG GG AP084 TT GG GT TT AA AA AA AA GA CC GG GG AP085 TT GG GT TT AA AA AA AA GG CC GG GG AP086 TT GA TT TT AA GG AA AA GG CC GG GG AP087 TT GG GT TT AG AA AA AA GG CC GG GG AP088 TT GG GT TT AA AA AA AT GG CC GG GG AP089 TT AA GT TC AA AA AC AA GG CC GG GG AP090 TT GG GG TT AA AA AA AA GG CC GG GG AP095 TT GG GG TC AA AA AA AA GA CG GT GA AP101 TA GG GT TT AA AA AA AT GG CC GG GG AP103 TA GA GT TT AA AG AA AT GA CC GG GG AP104 TT AA GG TT AA AG AA AT GG CC GG GG AP105 TA GA GT TT AA AG AA AT GA CC GG GG AP107 TA GG GG TT GG AG AA AT GA CC GG GG AP110 TT AA GT TC AA AG AA AT GG CC GG GG AP113 TT GA GG TT AA AG AA AA GG CG GG GG AP143 TA GA GT TT AA AG AA AT GA CC GG GG AP146 TT GG GG TC AA AA AA AA GA CG GT GA AP149 TT GG GG TC AA AA AA AA GA CG GT GA

[0099] 품종 / SNP AG_A081 AG_A082 AG_A089 AG_A095 AG_A096 AG_A097 AG_A098 AG_A100 AG_A102 AG_A105 AG_A106 AG_A108 allele G / C T / C C / T A / G G / T T / C C / T A / G G / T C / A C / T T / C AP001 GG TT CT AA GG TC CC AA GG CA CC TC AP002 GG TT CC AA GT TC CC AA GT CC CC TT AP003 GG TT CC AA GT TC CC AA GT CC CC TT AP004 GG TT CT AA GG TC CC AA GG CA CC TT AP005 GG TT CC AA GG TC CC AA GT CC CC TT AP006 GG TT CC AA GT TC CC AG GG CC CC TT AP007 GC TT CT AA GG TC CT AA GG CC CC TT AP008 GC TT CC GG GG TT CC AA GG CA CC TT AP009 GC TT CT AA GG TC CC AA GG CC CC TT AP010 GC TT CC AG GG TC CC AA GG CC CT TT AP011 GG TC CC AA GG TC CC AA GG CC CC TT AP012 GG TT CC AA GT TC CC AA GT CC CC TT AP013 GG TT CC AA GT TC CC AA GT CC CC TT AP014 GG TT CC AG GT TC CC AA GG CA CT TT AP015 GG TT CT AA GG TC TT AA GG CC CC TT AP016 GG TT CC AA GT TC CC AA GT CC CC TT AP017 GG TT CC AA GT TC CC AA GT CC CC TT AP018 GC TT CC AG GG TC CC AA GG CC CT TT AP019 GC TT CT AA GT TC CC AA GT CA CT TT AP020 GC TT CC AG GG TC CT AG GG CA TT TT AP021 GC TT CC AA GG TC CC AA GG CC CC TT AP022 GG TC CC AA GT TC CC AA GG CC CC TT AP023 GC TC CT AA GT TC CC AA GG CC CC TT AP024 GC TT CT AA GT TT CC AG GT CC CC TT AP025 GG TT CC AA GT TC CT AG GT CA CT TT AP026 GG TT CT AA GG TC TT AA GG CC CC TT AP027 GC TT CT AG GT TC CC AA GG CC CC TT AP028 CC TT CT AG GG TT CC AA GG CC CT TT AP029 GC TT TT AG GG TT CC AA GG CC CC TT AP030 GC TT CC AA GG TC CT AA GT CA CC TT AP031 GG TT CC AA GG TC CT AG GG CA CC TT AP032 GG TT CC AA GT TC CC AA GT CC CC TT AP033 GC TT CC AA GG TT CC AA GT CC CC TT AP034 GG TT CC AA GG TC CC AG GG CC CC TC AP035 GG TT CC AG GT TC CC AA GG AA CC TT AP036 GC TT CT AA GT TC CC AA GG CC CC TT AP037 GG TT CC AA GT TC CC AA GT CC CC TT AP038 GC TT CT AG GG TC CC AA GT CC CT TT AP039 GG TT CC AA GT TC CC AA GT CC CC TT AP040 GG TT CC AA GT TT CC AA GT CC CC TT AP041 GG TT CT AA GG TC CC AA GG CA CT TT AP042 GG TC CT AA GG TC CC AA GG CA CC TT AP043 GG TT CC AA GT TC CC AA GT CC CC TT AP044 GG TT CC AA GT TC CC AA GT CC CC TT AP045 GG TT CC AA GG TC CC AA GG CC CC TT AP046 GG TT CC AA GG TC CC AA GT CC CC TC AP047 GG TT CC AA GG TC CC AA GG CC CC TT AP048 GC TC CT AA GG TC CC AA GG CA CC TC AP049 GG TC CC AA GG TC CC AA GG CC CC TT AP050 GC TT CC AA GT TC CT AA GG CA CC TT AP051 GG TT CC AA GT TC CC AA GT CC CC TT AP052 GG TC CC AA GG TC CC AA GG CA CC CC AP053 GC TC CT AA GG TC CT AA GG CC CC TT AP054 GC TT CC AA GG TC CT AA GG CC CC TT AP055 GG TT CC AA GT TC CC AG GT CC CC TT AP056 GG TT CC AG GT TC CC AG GT CA CC TT AP057 GG TT CC AA GG TC CC AA GT CC CC TT AP058 GC TT CC AG GG TC CT AG GG CA CT TC AP059 GC TT CC AA GG TC CT AA GG CA CC TT AP060 GC TT CT AA GG TC CT AA GG CC CC TT AP061 GG TT CC AA GG TC CC AA TT CC CC TT AP062 GG TT CC AG TT CC CC AG GG CA CT TT AP063 GC TT CC AA GG TC CT AA GG CC CC TT AP064 GG TT CC AA GG TC CT AA GG CC CC TC AP065 GG TT CC AG GT TC CC AG GG CC CC TT AP066 GG TT CC AA GT TC CC AG GG CA CC TT AP067 GG TT CC AA GT TC CC AA GT CC CC TT AP068 GG TT CT AA GG TT CC AA GG CC CC TC AP069 GG TT CC AA GG TC CC AG GG CA CC TT AP080 CC TT CC AG GG TC CC AA GT CC CT TT AP083 GG TT CC AA GG TC CC AA GT CC CC TT AP084 GC TT CC AA TT TC CC AA GT CC CC TT AP085 GC TT CC AA GG TC CC AA GG CC CC TC AP086 GG TC CC AA GG TC CC AA TT CC CC TT AP087 GG TT CC AA GG TC CC AG TT CC CT TT AP088 GG TT CC AA GG TC CC AA GT CC CC TT AP089 GG TT CC AA GG TC CC AA GT CC CC TT AP090 GG TT CC AA GG TC CC AA GG CC CC TC AP095 CC TT CT AG GG TT CC AA GG CC CT TT AP101 GC TT CC AA GG TC CT AA GG CC CC TT AP103 GG TT CC AA GT TC CC AA GT CC CC TT AP104 GG TT CC AA GG TC CC AA GG CC CC TC AP105 GG TT CC AA GT TC CC AA GT CC CC TT AP107 GG TT CC AA GT TC CC AG GT CC CC TT AP110 GG TT CC AA GT TC CT AG GT CC CT TT AP113 GG TT CC AA GT TC CC AA GT CC CC TC AP143 GG TT CC AA GT TC CC AA GT CC CC TT AP146 CC TT CT AG GG TT CC SECOND GG CC CT TT AP149 CC TT CT AG GG TT CC SECOND GG CC CT TT

[0100] 품종 / SNP AG_A110 AG_A118 AG_A127 AG_A130 AG_A131 AG_A135 AG_B003 AG_B004 AG_B005 AG_B008 AG_B016 AG_B017 allele C / A A / G C / A C / T C / T T / C A / G C / A ARE THOUGH C / A ARE AP001 CC SECOND CC CC CC TT GG SECOND ARE GG CA SECOND AP002 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP003 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP004 CC SECOND CC CC CC TT GG CA ARE GG CA SECOND AP005 CC SECOND CC CC CC TT AG CA TT THOUGH CC TT AP006 CC SECOND CC CC CC TT AG CC TT SECOND CC TT AP007 CC SECOND CC CC CC TT GG CA TT SECOND CC TT AP008 CC AG CC CC CC TT SECOND CC SECOND THOUGH CC ARE AP009 CC SECOND CC CC CC TT GG CC TT THOUGH CA ARE AP010 CA AG CA CC CT TT SECOND CC SECOND THOUGH CC ARE AP011 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP012 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP013 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP014 CC AG CC CT CC TT SECOND CC SECOND THOUGH CC ARE AP015 CC SECOND CC CC CC TT AG CA TT SECOND CC TT AP016 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP017 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP018 CC AG CA CT CC TT GG CC SECOND GG CC SECOND AP019 CC SECOND CC CC CC TT AG CC ARE GG CC TT AP020 CC SECOND CA CC CC TT SECOND CC SECOND GG CC ARE AP021 CC SECOND CC CC CC TT GG CA TT SECOND CC TT AP022 CC SECOND CC CC CC TT GG CA TT GG CC TT AP023 CC SECOND CC CC CC TT GG CC TT GG CC TT AP024 CC SECOND CC CC CC TT AG CC TT SECOND CA ARE AP025 CC SECOND CC CC CC TT SECOND CC ARE SECOND CC ARE AP026 CC SECOND CC CC CC TT AG CA TT SECOND CC TT AP027 CC SECOND CC CC CT TT SECOND CC ARE THOUGH CC TT AP028 SECOND SECOND CA CT CT TT SECOND CC SECOND THOUGH CC ARE AP029 CC SECOND CC CT CC TT AG CC ARE GG CA TT AP030 CA SECOND CC CC CC TT SECOND CC TT GG CC TT AP031 CC SECOND CC CC CC TT SECOND CC ARE THOUGH CC TT AP032 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP033 CC SECOND CC CC CC TT AG CC TT THOUGH CA ARE AP034 CC SECOND CC CC CT TT GG CC SECOND SECOND CC TT AP035 CC SECOND CC CC CC TT GG CC ARE GG CC ARE AP036 CC SECOND CC CC CC TT AG CA TT THOUGH CC TT AP037 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP038 CC AG CC CT CC TC SECOND CC ARE THOUGH CC ARE AP039 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP040 CC SECOND CC CT CC TT SECOND CC ARE GG CC TT AP041 CC SECOND CC CT CC TC GG CC ARE GG CC TT AP042 CC SECOND CC CT CC TC AG CA TT GG CC TT AP043 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP044 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP045 CC SECOND CC CT CC TC GG CC SECOND THOUGH CC ARE AP046 CC SECOND CC CT CT TC SECOND CC SECOND THOUGH CC TT AP047 CC SECOND CA CC CT TT GG CC ARE GG CC ARE AP048 CC SECOND CC CC CC TT AG SECOND ARE GG CA ARE AP049 CC SECOND CC CC CC TT AG CA ARE THOUGH CC TT AP050 CA SECOND CC CC CC TT SECOND CC ARE THOUGH CC TT AP051 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP052 CC SECOND CC CC CC TT GG CA SECOND GG CA ARE AP053 CC SECOND CC CC CC TT AG CC ARE THOUGH CA TT AP054 CC SECOND CC CC CC TT AG CC ARE THOUGH CC TT AP055 CC SECOND CC CC CC TT SECOND CC TT SECOND CC TT AP056 CC SECOND CA CC CC TT SECOND CC TT GG CC TT AP057 CC SECOND CA CT CC TT AG CC TT GG CC TT AP058 CC AG CC CC CT TT AG CC ARE SECOND CC ARE AP059 CC SECOND CA CC CC TT AG CC ARE THOUGH CC TT AP060 CC SECOND CC CC CC TT GG CA TT SECOND CC TT AP061 CC SECOND CC CC CC TT SECOND CC TT GG CC ARE AP062 CA SECOND CC CC CC TT SECOND CC ARE GG CC TT AP063 CC SECOND CC CC CC TT AG CC ARE THOUGH CC TT AP064 CC SECOND CC CC CC TC SECOND CC SECOND GG CC TT AP065 CA SECOND CC CC CC TT SECOND CC ARE THOUGH CC TT AP066 CC SECOND CA CC CC TT AG CC ARE GG CC TT AP067 CC SECOND CC CT TT TT AG CC ARE THOUGH CC ARE AP068 CC AG CC CC CC TT AG CA ARE GG CA SECOND AP069 CC SECOND CC CC CC TT GG CC SECOND SECOND CC ARE AP080 CA SECOND CA CT CC TC AG CC ARE GG CC ARE AP083 CC SECOND CC CC TT TT GG CC SECOND THOUGH CC TT AP084 CA SECOND CC CC CT TC GG CC SECOND THOUGH CC TT AP085 CC SECOND CC CC TT TT AG CC SECOND SECOND CC TT AP086 CC SECOND CC CC TT TC GG CC SECOND THOUGH CC TT AP087 CC SECOND CC CC CT TT AG CC ARE SECOND CC TT AP088 CC SECOND CC CT CT TC GG CC ARE THOUGH CC ARE AP089 CC SECOND CC CC CT TC SECOND CC ARE SECOND CC TT AP090 CC SECOND CC CC TT TT GG CC SECOND SECOND CC TT AP095 SECOND SECOND CA CT CT TT SECOND CC SECOND THOUGH CC ARE AP101 CA SECOND CC CC CT TT AG CC ARE THOUGH CC TT AP103 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP104 CA SECOND CA CC CT TT GG CC ARE GG CC ARE AP105 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP107 CC SECOND CC CC CC TT AG CA TT SECOND CC TT AP110 CC SECOND CC CC CT TT AG CC TT GG CC TT AP113 CC SECOND CA CC CT TT AG CC SECOND SECOND CC TT AP143 CC SECOND CC CC CC TT SECOND CC TT GG CC TT AP146 SECOND SECOND CA CT CT TT SECOND CC SECOND THOUGH CC ARE AP149 SECOND SECOND CA CT CT TT SECOND CC SECOND THOUGH CC ARE

[0101] 품종 / SNP AG_B018 AG_B019 AG_B020 AG_B021 AG_B032 AG_B037 AG_B040 AG_B043 AG_B058 AG_B059 AG_A045 AG_A069 allele A / G ARE C / T THOUGH A / G C / T A / G T / C A / G T / C A / T A / G AP001 SECOND TT TT GG AG CC SECOND CC SECOND TC SECOND SECOND AP002 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP003 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP004 SECOND TT TT THOUGH GG CC AG TC SECOND TC SECOND AG AP005 SECOND TT CC GG SECOND CC SECOND TC SECOND TT SECOND SECOND AP006 SECOND TT CT GG SECOND CC SECOND TT SECOND TC AT SECOND AP007 SECOND TT CC THOUGH SECOND CC AG TC SECOND TT SECOND SECOND AP008 SECOND TT CC GG SECOND CT SECOND TT SECOND TT AT SECOND AP009 AG TT CC THOUGH SECOND CC AG TT SECOND TT SECOND SECOND AP010 SECOND TT CC GG SECOND TT SECOND TC SECOND TT AT SECOND AP011 SECOND ARE CC GG SECOND CC SECOND TT SECOND TT SECOND SECOND AP012 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP013 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP014 SECOND TT CC GG SECOND CT SECOND TC SECOND TT AT SECOND AP015 SECOND TT CT THOUGH AG CC AG TC SECOND TC SECOND SECOND AP016 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP017 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP018 SECOND TT CT GG SECOND CC SECOND TC SECOND TT AT GG AP019 SECOND ARE CC THOUGH SECOND CC AG TT AG TT SECOND SECOND AP020 SECOND TT CT GG SECOND CC SECOND TT SECOND TT AT SECOND AP021 SECOND TT CC GG AG CC SECOND TT SECOND TC SECOND SECOND AP022 SECOND TT CC GG AG CC SECOND TC SECOND TT AT SECOND AP023 SECOND TT CC GG SECOND CC AG TT SECOND TT AT SECOND AP024 AG TT CC THOUGH SECOND CC AG TT SECOND TT SECOND SECOND AP025 SECOND ARE CT GG SECOND CC SECOND TT AG TT AT GG AP026 SECOND TT CT THOUGH AG CC AG TC SECOND TC SECOND SECOND AP027 SECOND TT CC GG SECOND CC SECOND TC SECOND TC SECOND SECOND AP028 SECOND TT CC GG SECOND CT SECOND TC SECOND TT SECOND SECOND AP029 AG TT CC THOUGH SECOND CC AG TT SECOND TT SECOND SECOND AP030 SECOND ARE CC GG SECOND CC SECOND TT SECOND TT SECOND SECOND AP031 SECOND TT TT GG AG CC SECOND TT SECOND TC SECOND SECOND AP032 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP033 AG ARE CC GG SECOND CC SECOND TT SECOND TT SECOND SECOND AP034 SECOND ARE CC GG AG CC SECOND TT AG TC SECOND SECOND AP035 SECOND TT CC GG SECOND CC SECOND TT SECOND TT AT AG AP036 SECOND TT CC THOUGH SECOND CC AG TT AG TT SECOND SECOND AP037 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP038 SECOND TT CC GG SECOND CC SECOND TC SECOND TT SECOND SECOND AP039 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP040 SECOND TT CC GG SECOND CC SECOND TC SECOND TT SECOND SECOND AP041 SECOND ARE TT SECOND SECOND CC AG TT SECOND TT SECOND SECOND AP042 SECOND TT CT SECOND SECOND CC AG TC SECOND TT SECOND SECOND AP043 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP044 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP045 SECOND TT TT GG AG CC SECOND TT SECOND TC SECOND SECOND AP046 SECOND ARE TT GG SECOND CC SECOND TT GG TT SECOND SECOND AP047 SECOND TT TT GG AG CC SECOND TC SECOND TT SECOND AG AP048 AG TT CT THOUGH SECOND CC AG TC SECOND TT SECOND SECOND AP049 SECOND TT CC GG SECOND CC SECOND TT SECOND TT SECOND SECOND AP050 SECOND TT CC GG SECOND CC SECOND TT SECOND TT AT SECOND AP051 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP052 AG TT CT GG AG CC SECOND TC AG TT SECOND SECOND AP053 SECOND TT CT THOUGH AG CC AG TC SECOND TC AT SECOND AP054 SECOND TT CT GG AG CC SECOND TT SECOND TC AT SECOND AP055 SECOND TT CT GG SECOND CC SECOND TT SECOND TC SECOND SECOND AP056 SECOND ARE CT GG AG CC SECOND TT AG TC SECOND SECOND AP057 SECOND TT CT GG SECOND CC SECOND TT AG TT SECOND SECOND AP058 SECOND TT CT GG AG CT SECOND TC AG TT AT AG AP059 SECOND TT CT GG AG CC SECOND TT AG TC AT SECOND AP060 SECOND TT CC THOUGH SECOND CC AG TC SECOND TT SECOND SECOND AP061 SECOND ARE CT GG AG CT SECOND TT AG TT SECOND SECOND AP062 SECOND TT CT GG AG CC SECOND TT SECOND TT SECOND SECOND AP063 SECOND TT CT GG AG CC SECOND TT SECOND TC AT SECOND AP064 SECOND TT CC GG GG CT SECOND TT GG TT SECOND SECOND AP065 SECOND TT CT GG AG CC SECOND TT SECOND TT AT SECOND AP066 SECOND TT TT GG AG CC SECOND TT AG TC SECOND SECOND AP067 SECOND TT CT GG GG TT SECOND TT AG TC AT SECOND AP068 GG TT CT THOUGH SECOND CT AG TC SECOND TT SECOND SECOND AP069 SECOND TT CT GG AG CC SECOND TT SECOND TC SECOND AG AP080 SECOND TT CC GG SECOND TT SECOND TC SECOND TC AT SECOND AP083 SECOND TT TT GG GG CC SECOND TT AG TT SECOND SECOND AP084 SECOND TT CT GG AG CC SECOND TT AG TC AT SECOND AP085 AG ARE TT GG AG CT SECOND TT GG TT SECOND SECOND AP086 SECOND TT TT GG GG CT SECOND TC SECOND TC TT AG AP087 SECOND TT TT GG AG CT SECOND TT SECOND TT SECOND SECOND AP088 SECOND TT TT GG AG CC SECOND TT AG TC AT SECOND AP089 SECOND TT CT GG AG CC SECOND TC AG TT SECOND SECOND AP090 SECOND TT TT GG GG CC SECOND TT AG TT SECOND SECOND AP095 SECOND TT CC GG SECOND CT SECOND TC SECOND TT SECOND SECOND AP101 SECOND SECOND TT GG SECOND CT SECOND TT AG TT SECOND AG AP103 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP104 SECOND TT TT GG GG CC SECOND TT SECOND TC AT AG AP105 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP107 SECOND TT CT GG SECOND CC SECOND TC SECOND TC SECOND SECOND AP110 SECOND ARE CT GG SECOND CC SECOND TT AG TT AT GG AP113 SECOND SECOND TT GG AG CT SECOND TC SECOND TT AT GG AP143 SECOND ARE CC GG SECOND CC SECOND TT AG TT SECOND SECOND AP146 SECOND TT CC GG SECOND CT SECOND TC SECOND TT SECOND SECOND AP149 SECOND TT CC GG SECOND CT SECOND TC SECOND TT SECOND SECOND

[0102] 품종 / SNP AG_A090 AG_A104 AG_A109 AG_A116 AG_A124 AG_A133 AG_B033 AG_B034 AG_B046 AG_B052 AG_B055 AG_B060 allele ARE A / C A / G C / G C / A C / G C / G T / C A / T ARE C / T C / T AP001 ARE SECOND AG CG CC CC GG TT AT TT CC CC AP002 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP003 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP004 TT SECOND AG CG CC CC CG TT AT TT CC CC AP005 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CT AP006 TT SECOND SECOND CC CC CC CC TT SECOND TT CT CT AP007 TT SECOND SECOND CG CC CC CC TT SECOND TT CT CT AP008 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP009 TT SECOND SECOND CC CC CC CG TT AT ARE CT CC AP010 TT SECOND AG CC CC CC CC TT SECOND TT CT CC AP011 TT SECOND AG CG CC CC CC TT SECOND TT CC CC AP012 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP013 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP014 TT AC AG CC CC CC CC TT SECOND TT CT CC AP015 TT SECOND SECOND CC CC CC CC TT SECOND TT TT TT AP016 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP017 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP018 TT SECOND AG CC CC CC CC TT SECOND TT CC CT AP019 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CT AP020 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CT AP021 TT SECOND SECOND CG CC CG CC TT SECOND TT CT CT AP022 TT SECOND AG CG CC CC CG TT AT ARE CT CT AP023 TT SECOND SECOND CG CC CC GG TT AT ARE CC CC AP024 TT SECOND SECOND CC CC CC CG TT AT TT CT CC AP025 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP026 TT SECOND SECOND CC CC CC CC TT SECOND TT TT TT AP027 TT SECOND SECOND CC CC CC GG TT TT TT CC CC AP028 TT SECOND AG CC CC CC CC TT SECOND TT CC CT AP029 TT SECOND AG CC CC CC CC TT SECOND TT CC CC AP030 TT AC SECOND CC CC CC CC TT SECOND TT CT CC AP031 TT AC SECOND CG CC CC CC TT SECOND TT CT CC AP032 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP033 TT SECOND SECOND CC CC CC CC TT SECOND TT CT CC AP034 ARE AC SECOND CC CA CC CG TT SECOND TT CC CT AP035 ARE AC AG CC CA CC CC TT SECOND TT CT CT AP036 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP037 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP038 TT SECOND SECOND CC CC CC CC TT SECOND TT CT CT AP039 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP040 ARE SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP041 TT SECOND SECOND CG CC GG CG TC AT SECOND CT TT AP042 TT SECOND SECOND CG CC GG CG TC TT SECOND CC CT AP043 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP044 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP045 ARE SECOND SECOND CC SECOND CC CC TC SECOND TT TT CC AP046 TT SECOND SECOND CC CC CC CC TC SECOND TT CC CT AP047 TT SECOND SECOND CC CC CC CG TC TT TT CC CT AP048 TT SECOND AG CG CC CG GG TT AT ARE CC CC AP049 TT SECOND AG CG CC CG GG TT AT ARE CT CC AP050 TT AC AG CC CC CC CC TT SECOND TT TT CT AP051 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP052 ARE SECOND GG CC CC CC - TT SECOND TT CC CC AP053 TT SECOND SECOND CG CC CG CC TT SECOND TT CT CT AP054 TT SECOND SECOND CC CC CC CC TT SECOND TT TT CT AP055 TT SECOND SECOND CC CC CC CG TT AT TT CC CC AP056 TT SECOND SECOND CC CC CC CC TT SECOND TT CT CC AP057 TT SECOND AG CC CC CC CC TC SECOND TT CT CT AP058 TT SECOND SECOND CC CC CC - TT SECOND TT CT CT AP059 TT SECOND SECOND CC CC CC CC TT SECOND TT CT CT AP060 TT SECOND SECOND CG CC CC CC TT SECOND TT CT CT AP061 ARE SECOND AG CC CC CC CC TT SECOND TT CC CC AP062 TT AC AG CC CC CC CC TT SECOND TT CT CT AP063 TT SECOND SECOND CC CC CC CC TT SECOND TT TT CT AP064 TT SECOND SECOND CC SECOND CG CC TT SECOND TT CT CC AP065 TT SECOND AG CC CC CC CC TT SECOND TT CT TT AP066 ARE SECOND SECOND CC CC CC CG TT AT TT CT CC AP067 TT SECOND SECOND CG CA CG - TT SECOND TT CC CT AP068 TT AC SECOND CC CA CC - TT SECOND TT CC CT AP069 ARE AC SECOND CC CA CC CC TT SECOND TT CT CC AP080 ARE SECOND SECOND CC CC CC CC TT SECOND TT CT CT AP083 TT SECOND SECOND CG CA CC GG TT TT SECOND CC CC AP084 ARE SECOND SECOND CG CA CC GG TT AT ARE CC CC AP085 TT SECOND SECOND CC CA CC GG TT AT ARE CC CC AP086 TT SECOND SECOND CC CC CC CC TT SECOND TT CT CC AP087 ARE SECOND SECOND CG CA CC GG TT AT ARE CC CC AP088 ARE SECOND SECOND CC SECOND CC CG TT TT SECOND CC CT AP089 TT SECOND SECOND CC CC CC CC TC SECOND TT CC CC AP090 TT SECOND SECOND CC CA CC GG TT TT SECOND CC CC AP095 TT SECOND AG CC CC CC CC TT SECOND TT CC CT AP101 TT SECOND AG CC CC CG CC TT SECOND ARE CC CC AP103 TT SECOND SECOND CC CC CC CC TT SECOND TT CC CC AP104 ARE AC SECOND CG CA CC CC TT SECOND TT CT CC AP105 TT SECOND SECOND CC CC CC CC TT AA TT CC CC AP107 TT AA AA CC CC CC CG TT AT TT CT CT AP110 TA AA AA CC CA CC CC TT AA TT CC CC AP113 TT AA AA CC CA CC GG TT AA TT CC CT AP143 TT AA AA CC CC CC CC TT AA TT CC CC AP146 TT AA AG CC CC CC CC TT AA TT CC CT AP149 TT AA AG CC CC CC CC TT AA TT CC CT

[0103] By combining the above examples, it was found that the set of 96 SNP markers in Table 3 can be utilized as genetic markers with excellent apple variety identification ability. In addition, it was found that a large number of apple varieties can be identified with high accuracy using the primer set in Table 4, which is capable of detecting the SNP markers in Table 3.

[0105] The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art will understand that other specific forms can be easily modified without altering the technical spirit or essential features of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive.

Claims

Claim 1 A primer set for apple variety identification comprising each primer set for detecting a single nucleotide polymorphism (SNP) located at the 201st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs 1 to 96. Claim 2 In claim 1, each primer set for detecting the single nucleotide polymorphism comprises: a first primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 97 to 100; a second primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 101 to 104; a third primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 105 to 108; a fourth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 109 to 112; a fifth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 113 to 116; a sixth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 117 to 120; a seventh primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 121 to 124; and an eighth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 125 to 128. A ninth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 129 to 132; a tenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 133 to 136; an eleventh primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 137 to 140; a twelfth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 141 to 144; a thirteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 145 to 148; a fourteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 149 to 152; a fifteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 153 to 156; and a sixteenth primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 157 to 160.A 17th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 161 to 164; an 18th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 165 to 168; a 19th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 169 to 172; a 20th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 173 to 176; a 21st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 177 to 180; a 22nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 181 to 184; a 23rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 185 to 188; a 24th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 189 to 192; A 25th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 193 to 196; a 26th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 197 to 200; a 27th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 201 to 204; a 28th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 205 to 208; a 29th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 209 to 212; a 30th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 213 to 216; a 31st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 217 to 220; a 32nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 221 to 224; A 33rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 225 to 228;A 34th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 229 to 232; a 35th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 233 to 236; a 36th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 237 to 240; a 37th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 241 to 244; a 38th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 245 to 248; a 39th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 249 to 252; a 40th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 253 to 256; a 41st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 257 to 260; A 42nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 261 to 264; a 43rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 265 to 268; a 44th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 269 to 272; a 45th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 273 to 276; a 46th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 277 to 280; a 47th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 281 to 284; a 48th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 285 to 288; a 49th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 289 to 292; A 50th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 293 to 296;A 51st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 297 to 300; a 52nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 301 to 304; a 53rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 305 to 308; a 54th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 309 to 312; a 55th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 313 to 316; a 56th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 317 to 320; a 57th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 321 to 324; a 58th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 325 to 328; A 59th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 329 to 332; a 60th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 333 to 336; a 61st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 337 to 340; a 62nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 341 to 344; a 63rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 345 to 348; a 64th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 349 to 352; a 65th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 353 to 356; a 66th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 357 to 360; Primer set 67 consisting of four types of oligonucleotides represented by SEQ ID NOs 361 to 364;Primer set 68 consisting of four types of oligonucleotides represented by SEQ ID NOs 365 to 368; Primer set 69 consisting of four types of oligonucleotides represented by SEQ ID NOs 369 to 372; Primer set 70 consisting of four types of oligonucleotides represented by SEQ ID NOs 373 to 376; Primer set 71 consisting of four types of oligonucleotides represented by SEQ ID NOs 377 to 380; Primer set 72 consisting of four types of oligonucleotides represented by SEQ ID NOs 381 to 384; Primer set 73 consisting of four types of oligonucleotides represented by SEQ ID NOs 385 to 388; Primer set 74 consisting of four types of oligonucleotides represented by SEQ ID NOs 389 to 392; Primer set 75 consisting of four types of oligonucleotides represented by SEQ ID NOs 393 to 396; A 76th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 397 to 400; a 77th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 401 to 404; a 78th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 405 to 408; a 79th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 409 to 412; an 80th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 413 to 416; an 81st primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 417 to 420; an 82nd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 421 to 424; and an 83rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 425 to 428; A 84th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 429 to 432;A primer set of 85 consisting of four types of oligonucleotides represented by SEQ ID NOs 433 to 436; a primer set of 86 consisting of four types of oligonucleotides represented by SEQ ID NOs 437 to 440; a primer set of 87 consisting of four types of oligonucleotides represented by SEQ ID NOs 441 to 444; a primer set of 88 consisting of four types of oligonucleotides represented by SEQ ID NOs 445 to 448; a primer set of 89 consisting of four types of oligonucleotides represented by SEQ ID NOs 449 to 452; a primer set of 90 consisting of four types of oligonucleotides represented by SEQ ID NOs 453 to 456; a primer set of 91 consisting of four types of oligonucleotides represented by SEQ ID NOs 457 to 460; a primer set of 92 consisting of four types of oligonucleotides represented by SEQ ID NOs 461 to 464; A primer set for identifying apple varieties, comprising: a 93rd primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 465 to 468; a 94th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 469 to 472; a 95th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 473 to 476; and a 96th primer set consisting of four types of oligonucleotides represented by SEQ ID NOs 477 to 480. Claim 3 A primer set for identifying apple varieties according to claim 2, wherein each of the 1st to 96 primer sets identifies the genotype of each corresponding single nucleotide polymorphism position base in the table below: . Claim 4 An apple variety identification kit comprising a primer set for apple variety identification according to any one of claims 1 to 3. Claim 5 (a) a step of isolating genomic DNA from an apple; (b) a step of detecting a single nucleotide polymorphism of claim 1 in the isolated genomic DNA; and (c) a step of identifying an apple variety by analyzing the detection result; comprising a method for identifying an apple variety. Claim 6 A method for identifying an apple variety according to claim 5, wherein step (b) comprises a step of amplifying nucleic acid using the isolated genomic DNA as a template and using the set of primers for identifying apple varieties according to any one of claims 1 to 3 as primers. Claim 7 A method for identifying an apple variety according to claim 5, wherein step (c) comprises determining the genotype of the single nucleotide polymorphism position base of claim 1 in the isolated genomic DNA.

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