Cosmetic composition for skin moisturizing containing polysaccharides derived from tremella fuciformis and manufacturing method thereof and health functional food composition for improving intestinal health

Abstract

The present invention relates to a cosmetic composition for skin moisturization containing polysaccharides derived from white wood ear mushrooms, a method for manufacturing the same, and a health functional food composition for improving gut health. More specifically, by including polysaccharides derived from white wood ear mushrooms as an active ingredient, the invention can exhibit excellent skin moisturizing, wrinkle improvement, and antioxidant effects when utilized as a cosmetic composition, and can help improve gut health when utilized as a health functional food composition for improving gut health through excellent antioxidant and prebiotic activities.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition for skin moisturization containing polysaccharides derived from white wood ear mushrooms, a method for manufacturing the same, and a health functional food composition for improving gut health. More specifically, by including polysaccharides derived from white wood ear mushrooms as an active ingredient, the invention can exhibit excellent skin moisturizing, wrinkle improvement, and antioxidant effects when utilized as a cosmetic composition, and can help improve gut health when utilized as a health functional food composition for improving gut health through excellent antioxidant and prebiotic activities. Background Technology

[0002] With increasing interest in skin aging and health in modern society, the demand for safe and effective natural materials is surging. In particular, skin dryness is known to be a major cause of various skin aging phenomena, such as wrinkle formation and loss of elasticity, and moisturizing ingredients like hyaluronic acid are widely used to address this.

[0003] Furthermore, as oxidative stress caused by reactive oxygen species (ROS) is a key factor accelerating skin aging, the importance of antioxidants such as Vitamin C is being highlighted.

[0004] However, there were problems such as high production costs for hyaluronic acid, which is mainly extracted from microbial fermentation or animal tissues, and difficulties in formulation due to the low stability of vitamin C.

[0006] Meanwhile, as the concept of "Inner Beauty"—which posits that inner health leads to outer beauty—spreads, interest in promoting overall and skin health through the improvement of the gut microbiome is also rising. Along with probiotics, the importance of prebiotics, which serve as their food source, is being emphasized.

[0007] However, existing prebiotics are primarily limited to fructooligosaccharides and galactooligosaccharides, creating a need for the development of natural materials with novel functionalities. In the field of health functional foods as well, existing polysaccharide materials are limited to single functionalities, leading to an increasing need for natural materials possessing complex biological activities.

[0008] In particular, according to recent studies, white wood ear mushroom polysaccharides (TFP) are 10 6 It has been reported that moisture retention and film-forming ability on the skin surface are maximized when the molecules are in a high molecular weight form of Da or higher. However, existing extraction technologies use excessive enzymatic treatment or ultrasonic degradation solely to increase yield, which causes the polysaccharide chains to be cleaved into low molecular weight molecules (<10 6 There was a problem with Da). Accordingly, an optimal structure-preserving extraction process is required that maintains the natural triple-helical structure to simultaneously enhance skin moisturizing barrier formation ability and intestinal delivery rate. Prior art literature

[0009] Korean Registered Patent Publication No. 10-2015857 (Published Aug. 30, 2019) The problem to be solved

[0010] The objective of the present invention is to provide a cosmetic composition for skin moisturization containing a polysaccharide derived from white wood ear mushroom, a method for manufacturing the same, and a health functional food composition for improving gut health, which can exhibit excellent skin moisturizing and wrinkle improvement effects when utilized as a cosmetic composition by including the polysaccharide derived from white wood ear mushroom as an active ingredient, and can help improve gut health when utilized as a health functional food composition for improving gut health through excellent antioxidant and prebiotic activities. means of solving the problem

[0011] A cosmetic composition for skin moisturizing containing a polysaccharide derived from Tremella fuciformis according to an embodiment of the present invention for solving the above problem is characterized by containing a polysaccharide (TFP) derived from Tremella fuciformis as an active ingredient.

[0012] The above-mentioned polysaccharide derived from the white wood ear mushroom is a heteropolysaccharide in which glucuronic acid is bonded to a mannose backbone, and its weight-average molecular weight measured by gel permeation chromatography (GPC) is 1.5 × 10⁻⁶ 7 Up to 3.5 × 10 7 It is Da, has over 70% DPPH antioxidant activity and over 130% moisture content.

[0013] The above-mentioned polysaccharide derived from white wood ear mushrooms is included in an amount of 0.1 to 10 weight% based on the total weight of the cosmetic composition.

[0014] The above cosmetic composition is preferably at least one of a serum, cream, lotion, ampoule, and mask pack formulation.

[0016] A health functional food composition for improving intestinal health containing a polysaccharide derived from Tremella fuciformis according to an embodiment of the present invention for solving the above problem is characterized by containing a polysaccharide (TFP) derived from Tremella fuciformis as an active ingredient.

[0017] The above-mentioned polysaccharide derived from white wood ear mushrooms is 1.5 × 10 7 Up to 3.5 × 10 7 It has a molecular weight of Da, DPPH antioxidant activity of over 70%, and a moisture content of over 130%.

[0018] The above-mentioned polysaccharide derived from white wood ear mushrooms is included in an amount of 100 to 2,000 mg per daily intake.

[0019] The above health functional food composition is preferably at least one of capsule, granule, powder, pill, liquid, and jelly formulations.

[0021] A method for preparing a cosmetic composition for skin moisturization containing a polysaccharide derived from white wood ear mushroom according to an embodiment of the present invention for solving the above problem comprises: (A) a step of grinding dried white wood ear mushroom to 20 to 40 mesh; (B) a step of hot water extraction of the ground white wood ear mushroom at 80 to 95°C with 10 to 20 times the amount of purified water for 2 to 4 hours; (C) a step of filtering and vacuum concentrating the hot water extracted extract; (D) a step of powdering the vacuum-extracted product by spray drying to obtain a polysaccharide derived from white wood ear mushroom; and (E) a step of adding the polysaccharide derived from white wood ear mushroom to cosmetic raw materials and distilled water and stirring to form a cosmetic composition.

[0022] In step (D) above, the spray drying is carried out under conditions of 150 to 180°C.

[0023] The above-mentioned polysaccharide derived from white wood ear mushrooms is added in an amount of 0.1 to 10 weight% based on the total weight of the cosmetic composition. Effects of the invention

[0024] The cosmetic composition for skin moisturizing containing a polysaccharide derived from white wood ear mushroom according to the present invention, the method for manufacturing the same, and the health functional food composition for improving intestinal health exhibit the following excellent effects.

[0025] First, when polysaccharides derived from white wood ear mushrooms are applied to cosmetic compositions, they provide a combination of powerful moisturizing power (over 130%) surpassing hyaluronic acid, excellent antioxidant power (over 70%) comparable to vitamin C, and skin elasticity enhancement effects through the promotion of collagen synthesis (increase in fibroblast proliferation by over 30%), making them highly effective for comprehensively improving dry and aging skin. In particular, they exhibit significant moisturizing and antioxidant effects even at low concentrations such as 0.1 wt%, making them applicable to a wide range of cosmetic formulations; furthermore, minimal functional expression can be expected even at minute amounts such as 0.01 wt%, thereby enhancing the utility of the invention.

[0026] Above all, unlike hyaluronic acid which has a simple humectant mechanism, the TFP of the present invention simultaneously provides an occlusive effect in which polymer chains form a thin film on the skin surface. Through this, it was confirmed that the 'retention' which inhibits moisture evaporation to the external environment is maintained at a significantly higher level even after 24 hours compared to hyaluronic acid.

[0027] Second, when polysaccharides derived from white wood ear mushrooms are applied to health functional food compositions, they can contribute to improving gut health and boosting immunity by simultaneously performing prebiotic and antioxidant functions as a safe natural material that helps the growth of beneficial bacteria in the intestines. Even with a small amount of 100 mg per daily intake, they exhibit significant physiological activity, such as improving the intestinal microbial environment and reducing inflammation markers, which is advantageous for development and distribution in various forms of health functional foods.

[0028] Specifically, the polymer TFP of the present invention reaches the large intestine without being broken down by digestive enzymes and helps the selective proliferation of beneficial bacteria such as Lactobacillus. This induces the production of short-chain fatty acids (SCFAs) in the intestines, and the generated SCFAs move through the bloodstream to suppress inflammatory responses in the skin, acting as a mechanism to improve the 'Gut-Skin Axis'.

[0029] Therefore, the present invention exhibits complex anti-aging effects, such as skin moisturization, wrinkle improvement, and elasticity enhancement, through superior moisturizing power surpassing hyaluronic acid, antioxidant power comparable to Vitamin C, and an effect that promotes collagen synthesis. Furthermore, the present invention acts as a prebiotic to proliferate beneficial intestinal bacteria and improve gut health. In particular, the present invention demonstrates significant efficacy even at the proposed minimum content, thereby enhancing its value for industrial application. Accordingly, the TFP of the present invention can be highly usefully utilized in the fields of cosmetics and health functional foods as a safe natural material. Brief explanation of the drawing

[0030] FIG. 1 is a process flowchart showing a method for preparing a skin moisturizing cosmetic composition containing a polysaccharide derived from white wood ear mushroom according to an embodiment of the present invention. Specific details for implementing the invention

[0031] Hereinafter, preferred embodiments of the present invention and the physical properties of each component are described in detail. This description is intended to be sufficient for a person skilled in the art to easily practice the invention, and does not imply that the technical scope and concept of the present invention are limited thereby.

[0033] Generally, the white wood ear mushroom (*Tremella fuciformis*) has been traditionally used for food and medicine and has proven safety. Its polysaccharide (TFP) is known as plant-based hyaluronic acid due to its excellent water-retaining capacity. While existing studies have reported on the simple moisturizing effects of TFP, there is no precedent for comprehensively identifying specific moisturizing power surpassing hyaluronic acid, antioxidant power comparable to vitamin C, and the effect of promoting collagen synthesis through the proliferation of skin fibroblasts, thereby presenting the potential for comprehensive anti-aging as a topical skin agent.

[0034] In addition, studies on the effects of TFP on the intestinal environment have suggested the possibility that TFP may simultaneously possess the function of a prebiotic that promotes the growth of beneficial bacteria such as Lactobacillus and Ruminococcusae, and an anti-inflammatory effect that alleviates damage to the intestinal mucosa. However, attempts to utilize TFP as a core functional ingredient in health functional foods based on these effects of improving intestinal health are still minimal.

[0036] The present invention is intended to solve the problems mentioned above, and its purpose is as follows.

[0037] First, the present invention provides a highly moisturizing cosmetic composition having a superior moisture content (134.4%) compared to hyaluronic acid (125%).

[0038] In addition, the present invention provides an anti-aging cosmetic composition having an antioxidant effect (73%) at the level of Vitamin C (78%) and a collagen synthesis-promoting effect (fibroblast proliferation +32%).

[0039] In addition, the present invention provides a multifunctional health functional food composition having an effect of promoting the growth of beneficial bacteria in the intestines and an anti-inflammatory effect.

[0040] In addition, the present invention provides an optimized method for manufacturing the above compositions.

[0042] As a result of years of research to solve the aforementioned problems, the inventors confirmed that TFP extracted from white wood ear mushrooms under specific conditions exhibits complex anti-aging effects on the skin by possessing superior moisturizing power compared to hyaluronic acid, antioxidant power comparable to Vitamin C, and the ability to promote collagen synthesis. At the same time, by confirming that TFP demonstrates excellent effects in improving intestinal health through the proliferation of beneficial gut bacteria and the protection of the intestinal mucosa, the inventors discovered that utilizing it as an active ingredient in cosmetics and health functional foods could have outstanding industrial value, thereby completing the present invention.

[0044] To this end, the present invention provides a cosmetic composition and a health functional food composition comprising a polysaccharide (TFP) derived from the white wood ear mushroom (Tremella fuciformis) as an active ingredient. In addition, the present invention provides a method for preparing a cosmetic composition comprising a polysaccharide derived from the white wood ear mushroom.

[0046] 1. Cosmetic composition

[0047] A cosmetic composition for skin moisturizing containing a polysaccharide derived from Tremella fuciformis according to one embodiment of the present invention comprises a polysaccharide (TFP) derived from Tremella fuciformis as an active ingredient.

[0048] More specifically, a cosmetic composition for moisturizing the skin containing a polysaccharide derived from white wood ear mushroom according to one embodiment of the present invention contains 0.1 to 10 weight%, more preferably 3 to 7 weight%, of a polysaccharide derived from white wood ear mushroom (TFP) as an active ingredient based on 100 weight% of the total cosmetic composition.

[0049] If the content of polysaccharides derived from white wood ear mushrooms is less than 0.1% by weight, the moisturizing and anti-aging effects may be negligible. In addition, if the content of polysaccharides derived from white wood ear mushrooms exceeds 10% by weight, the texture of the formulation (stickiness, etc.) may be reduced, and the increase in effect with increasing content may be negligible, making it uneconomical.

[0050] The polysaccharide derived from white wood ear mushrooms (TFP) used in the present invention is prepared by crushing dried white wood ear mushroom fruiting bodies, adding 10 to 20 times the weight of the raw material in purified water, performing hot water extraction at 80 to 95°C for 2 to 4 hours, and then filtering, concentrating under reduced pressure, and spray drying.

[0051] The polysaccharide derived from Phellodendron amurense (TFP) prepared by this method has a heteropolysaccharide structure containing glucuronic acid, mannose, xylose, etc. In particular, the polysaccharide derived from Phellodendron amurense is a heteropolysaccharide in which glucuronic acid is bonded to a mannose backbone, and its weight-average molecular weight measured by gel permeation chromatography (GPC) is 1.5 × 10⁻⁶. 7 Up to 3.5 × 10 7 It is Da, possesses over 70% DPPH antioxidant activity, and has a moisture content of over 130%. More specifically, the polysaccharide derived from white wood ear mushroom is 1.5 × 10 7 Up to 3.5 × 10 7 It has a molecular weight of Da, 70 to 90% DPPH antioxidant activity, and 130 to 160% moisture content.

[0052] In the present invention, the polysaccharide derived from white wood ear mushroom (TFP) forms a moisture film on the skin surface through powerful moisturizing power that attracts more than 500 times its own weight in moisture, and inhibits moisture evaporation within the skin, thereby providing deep and long-lasting hydration. Furthermore, the polysaccharide derived from white wood ear mushroom (TFP) protects the skin by effectively removing free radicals, the main culprit of skin aging, through antioxidant activity represented by DPPH radical scavenging ability. Moreover, the polysaccharide derived from white wood ear mushroom (TFP) promotes the proliferation of fibroblasts in the dermal layer of the skin, thereby inducing the biosynthesis of collagen and elastin, which enhances the fundamental elasticity of the skin and exhibits the effect of improving wrinkles.

[0054] A cosmetic composition for moisturizing the skin containing a polysaccharide derived from white wood ear mushroom according to one embodiment of the present invention may additionally include, in addition to the polysaccharide derived from white wood ear mushroom (TFP), one or more selected from moisturizers, emollients, emulsifiers, thickeners, surfactants, pH adjusters, preservatives, fragrances, and colorants that are commonly used in cosmetic formulations.

[0055] In addition, a cosmetic composition for moisturizing the skin containing a polysaccharide derived from white wood ear mushroom according to one embodiment of the present invention may be at least one of a serum, cream, lotion, ampoule, and mask pack formulation, but is not particularly limited to the formulation. For example, the cosmetic composition may be manufactured in various forms such as skin lotion, milk lotion, nourishing cream, essence, serum, ampoule, mask pack, cleansing foam, etc.

[0057] 2. Health functional food composition

[0058] A health functional food composition for improving intestinal health comprising a polysaccharide derived from Tremella fuciformis according to another embodiment of the present invention comprises a polysaccharide (TFP) derived from Tremella fuciformis as an active ingredient.

[0059] More specifically, a health functional food composition for improving intestinal health containing a polysaccharide derived from white wood ear mushroom according to another embodiment of the present invention contains 100 to 2,000 mg of the polysaccharide derived from white wood ear mushroom per daily intake.

[0060] Polysaccharides derived from Phellinus igniarius (TFP) have a heteropolysaccharide structure containing glucuronic acid, mannose, xylose, etc. In particular, polysaccharides derived from Phellinus igniarius are 1.5 × 10⁻⁶ 7 Up to 3.5 × 10 7 It is a high-molecular-weight substance having a molecular weight of Da, DPPH antioxidant activity of over 70%, and a moisture content of over 130%. More specifically, the polysaccharide derived from white wood ear mushroom is 1.5 × 10 7 Up to 3.5 × 10 7 It has a molecular weight of Da, 70 to 90% DPPH antioxidant activity, and 130 to 160% moisture content.

[0061] In the present invention, the polysaccharide derived from white wood ear mushroom (TFP) reaches the large intestine without being broken down by digestive enzymes and exhibits prebiotic activity, acting as food for beneficial intestinal bacteria, particularly Lactobacillus and Ruminococcusae species. Through this, it improves the balance of the intestinal microbial community and promotes the production of short-chain fatty acids (SCFAs) generated by beneficial bacteria, thereby enhancing the health of intestinal mucosal cells and strengthening the intestinal barrier function. In addition, the anti-inflammatory and antioxidant effects of the polysaccharide derived from white wood ear mushroom (TFP) can help alleviate intestinal inflammatory responses and reduce systemic oxidative stress.

[0062] A health functional food composition for improving gut health containing a polysaccharide derived from white wood ear mushroom according to another embodiment of the present invention may additionally include one or more selected from food-acceptable excipients, diluents, sweeteners, flavorings, etc.

[0063] A health functional food composition for improving intestinal health containing a polysaccharide derived from white wood ear mushroom according to another embodiment of the present invention is not particularly limited in its formulation and can be manufactured in any form that is easy to consume, such as tablets, capsules, granules, powder, pills, liquid, or jelly.

[0065] Hereinafter, a method for preparing a cosmetic composition for skin moisturization containing a polysaccharide derived from white wood ear mushroom according to an embodiment of the present invention will be described.

[0066] FIG. 1 is a process flowchart showing a method for preparing a cosmetic composition for skin moisturization containing a polysaccharide derived from white wood ear mushroom according to an embodiment of the present invention.

[0067] As shown in FIG. 1, a method for preparing a skin moisturizing cosmetic composition containing a polysaccharide derived from white wood ear mushroom according to an embodiment of the present invention includes a grinding step (S1), a hot water extraction step (S2), a filtration and vacuum concentration step (S3), a spray drying step (S4), and a stirring step (S5).

[0069] Grinding step (S1)

[0070] In the grinding step (S1), the dried white wood ear mushrooms are ground to 20 to 40 mesh.

[0071] At this time, grinding may be performed using one or more methods selected from jet mills, ball mills, roller mills, etc., but is not limited thereto.

[0072] If the size of these white wood ear mushrooms is less than 20 mesh, it is undesirable because there is a concern that the process cost for grinding them into fine particles will be excessive. In addition, if the size of the white wood ear mushrooms exceeds 40 mesh, it is undesirable because they may clump together when mixed with purified water.

[0073] In addition, if the material is ground excessively finely beyond 40 mesh, the leaching of impurities such as starch and protein increases rapidly, which not only lowers the purity of the polysaccharides but also causes fouling in subsequent filtration processes due to the increased viscosity of the extract. Therefore, 20 to 40 mesh is a critical particle size range for efficiently obtaining high-purity polysaccharides.

[0075] hot water extraction step (S2)

[0076] In the hot water extraction step (S2), crushed white wood ear mushrooms are extracted with 10 to 20 times the amount of purified water at 80 to 95°C for 2 to 4 hours.

[0077] In this step, it is more preferable to perform hot water extraction at 85 to 95°C with 14 to 18 times the amount of purified water for 2.5 to 3.5 hours. At this time, if the extraction temperature exceeds 95°C or pressurized conditions are used, the glycosidic bonds of the polysaccharides may be hydrolyzed, causing a rapid decrease in molecular weight and the destruction of the triple helix structure. The temperature conditions of the present invention are optimal conditions that can maximize the extraction yield while maintaining the structural stability of the polysaccharides.

[0078] When performing such hot water extraction, it is desirable to stir at a speed of 50 to 200 rpm. If the stirring speed is less than 50 rpm, there is a risk that the crushed white wood ear mushrooms will not be uniformly mixed with the purified water, and thus the extraction will not proceed smoothly. In addition, if the stirring speed exceeds 200 rpm, it may act as a factor that only increases manufacturing costs without any further effect, making it uneconomical.

[0080] Filtration and vacuum concentration step (S3)

[0081] In the filtration and reduced pressure concentration step (S3), the hot water extracted extract is filtered and reduced pressure concentrated.

[0082] At this time, it is preferable to perform vacuum concentration using a vacuum concentrator at a concentration temperature of 30 to 45°C under a reduced pressure condition of 1 to 3 kgf / ㎠. Concentrating at a concentration temperature of 30 to 45°C under a reduced pressure condition of 1 to 3 kgf / ㎠ is intended to prevent foaming during the concentration process and to perform smooth concentration.

[0084] Spray drying step (S4)

[0085] In the spray drying step (S4), the product concentrated under reduced pressure is powdered by spray drying to obtain a polysaccharide derived from white wood ear mushrooms.

[0086] In this step, it is preferable to perform spray drying at a temperature of 150 to 180°C. If the spray drying temperature is below 150°C, there is a risk that sufficient drying will not occur, and proper pulverization will not take place. In addition, if the spray drying temperature exceeds 180°C, there is a risk that only manufacturing costs will increase without further increase in effectiveness, so this is not desirable.

[0087] Accordingly, the polysaccharide derived from *Typical Pulmonary Finger* (TFP) obtained has a heteropolysaccharide structure containing glucuronic acid, mannose, xylose, etc. In particular, the polysaccharide derived from *Typical Pulmonary Finger* is 1.5 × 10⁻⁶ 7 Up to 3.5 × 10 7 It is a high-molecular-weight substance having a molecular weight of Da, DPPH antioxidant activity of over 70%, and a moisture content of over 130%. More specifically, the polysaccharide derived from white wood ear mushroom is 1.5 × 10 7 Up to 3.5 × 10 7 It has a molecular weight of Da, 70 to 90% DPPH antioxidant activity, and 130 to 160% moisture content.

[0089] Stirring step (S5)

[0090] In the stirring step (S5), a polysaccharide derived from white wood ear mushrooms is added to cosmetic raw materials and distilled water and stirred to form a cosmetic composition.

[0091] In this step, it is preferable to add the polysaccharide derived from white wood ear mushroom in an amount of 0.1 to 10 weight% based on the total weight of the cosmetic composition, and more preferable to add it in an amount of 3 to 7 weight%. If the content of the polysaccharide derived from white wood ear mushroom is less than 0.1 weight%, the moisturizing and anti-aging effects may be negligible. In addition, if the content of the polysaccharide derived from white wood ear mushroom exceeds 10 weight%, the usability of the formulation (stickiness, etc.) is reduced, and the increase in effect with increasing content is negligible, so it may not be economical.

[0092] Here, the cosmetic ingredients may include one or more selected from moisturizers, emollients, emulsifiers, thickeners, surfactants, pH adjusters, preservatives, fragrances, colorants, etc., which are commonly used in cosmetic formulations.

[0093] In addition, the cosmetic composition may be at least one of a serum, cream, lotion, ampoule, and mask pack formulation, but is not particularly limited to the formulation. For example, the cosmetic composition may be manufactured in various forms such as skin lotion, milk lotion, nourishing cream, essence, serum, ampoule, mask pack, cleansing foam, etc.

[0095] As described above, the cosmetic composition for skin moisturizing containing a polysaccharide derived from white wood ear mushroom according to an embodiment of the present invention, the method for manufacturing the same, and the health functional food composition for improving intestinal health exhibit the following excellent effects.

[0096] First, when polysaccharides derived from white wood ear mushrooms are applied to cosmetic compositions, they provide a combination of powerful moisturizing power (over 130%) surpassing hyaluronic acid, excellent antioxidant power (over 70%) comparable to vitamin C, and skin elasticity enhancement effects through the promotion of collagen synthesis (increase in fibroblast proliferation by over 30%), making them highly effective for comprehensively improving dry and aging skin. In particular, they exhibit significant moisturizing and antioxidant effects even at low concentrations such as 0.1 wt%, making them applicable to a wide range of cosmetic formulations; furthermore, minimal functional expression can be expected even at minute amounts such as 0.01 wt%, thereby enhancing the utility of the invention.

[0097] Above all, unlike hyaluronic acid which has a simple humectant mechanism, the TFP of the present invention simultaneously provides an occlusive effect in which polymer chains form a thin film on the skin surface. Through this, it was confirmed that the 'retention' which inhibits moisture evaporation to the external environment is maintained at a significantly higher level even after 24 hours compared to hyaluronic acid.

[0098] Second, when polysaccharides derived from white wood ear mushrooms are applied to health functional food compositions, they can contribute to improving gut health and boosting immunity by simultaneously performing prebiotic and antioxidant functions as a safe natural material that helps the growth of beneficial bacteria in the intestines. Even with a small amount of 100 mg per daily intake, they exhibit significant physiological activity, such as improving the intestinal microbial environment and reducing inflammation markers, which is advantageous for development and distribution in various forms of health functional foods.

[0099] Specifically, the polymer TFP of the present invention reaches the large intestine without being broken down by digestive enzymes and helps the selective proliferation of beneficial bacteria such as Lactobacillus. This induces the production of short-chain fatty acids (SCFAs) in the intestines, and the generated SCFAs move through the bloodstream to suppress inflammatory responses in the skin, acting as a mechanism to improve the 'Gut-Skin Axis'.

[0100] Therefore, the present invention exhibits complex anti-aging effects, such as skin moisturization, wrinkle improvement, and elasticity enhancement, through superior moisturizing power surpassing hyaluronic acid, antioxidant power comparable to Vitamin C, and an effect that promotes collagen synthesis. Furthermore, the present invention acts as a prebiotic to proliferate beneficial intestinal bacteria and improve gut health. In particular, the present invention demonstrates significant efficacy even at the proposed minimum content, thereby enhancing its value for industrial application. Accordingly, the TFP of the present invention can be highly usefully utilized in the fields of cosmetics and health functional foods as a safe natural material.

[0102] Examples

[0103] Hereinafter, the structure and operation of the present invention will be explained in more detail through preferred embodiments of the present invention. However, these are presented as preferred examples of the present invention and should not be interpreted in any way as limiting the present invention.

[0104] Details not listed here can be sufficiently technically inferred by a person skilled in this field, so their explanation will be omitted.

[0106] 1. Evaluation of TFP's moisturizing ability

[0107] Table 1 shows the results of measuring the water absorption of TFP and hyaluronic acid, and Table 2 shows the results of applying TFP and hyaluronic acid to the human body.

[0109] 1) Purpose of the test

[0110] The purpose is to evaluate the skin moisturizing effect of polysaccharides (TFP: Tremella fuciformis polysaccharide) extracted from the white wood ear mushroom (Tremella fuciformis).

[0112] 2) Test Method

[0114] 2-1. Sample Preparation

[0115] Polysaccharide extract (TFP) of *Tremella fuciformis* with a molecular weight of 2.1 × 10⁻⁶ 7 Da, with a polysaccharide content of 48.5 wt% was prepared, and as a control, hyaluronic acid (molecular weight 1.5 × 10⁻⁶ 6 Da) was prepared. Afterwards, each sample was dissolved in purified water at concentrations of 0.1 wt%, 0.5 wt%, and 1.0 wt%.

[0117] 2-2. Measurement of Water Absorption Capacity

[0118] 0.5g of each sample was weighed into a separate crucible and stored in a glass desiccator along with a beaker containing water. Subsequently, weight changes were measured after 1, 2, 3, 4, 5, and 6 hours. The amount of absorbed water was calculated using the formula (weight at time - initial weight) / initial weight × 100.

[0120] 2-3. Human Application Test

[0121] Twenty healthy adult women (average age 33) were classified as subjects, and the inner skin of the forearm was measured. The measuring device used was the Corneometer® CM 825 (Courage + Khazaka electronic GmbH), the sample application amount was 0.003 mg / cm², and the measurement points were divided into before application, 1 hour, 3 hours, and 5 hours later.

[0123] [Table 1]

[0124]

[0126] [Table 2]

[0127]

[0128] As shown in Tables 1 and 2, when comparing the water absorption after 6 hours, it was confirmed that the Tremella fuciformis polysaccharide extract (TFP) had a higher water absorption rate than hyaluronic acid.

[0129] In addition, as a result of comparing and evaluating the improvement rate of skin moisture after 5 hours, it was confirmed that TPF showed superior moisture retention ability with improved skin moisture compared to hyaluronic acid.

[0131] 2. Evaluation of TFP's Antioxidant Activity (Measurement of DPPH Scavenging Activity)

[0132] Table 3 shows the results of measuring the DPPH radical scavenging ability of TFP, and Table 4 shows the results of measuring the ROS generation inhibition rate and the increase rate of antioxidant enzyme activity of TFP.

[0134] 1) Sample preparation

[0135] TFP solutions were prepared by dissolving them in distilled water at concentrations of 50, 100, 200, and 500 μg / mL, L-ascorbic acid (vitamin C) was prepared by dissolving it in distilled water at the same concentration as a positive control, and distilled water was prepared as a negative control.

[0137] 2) DPPH radical scavenging activity measurement

[0138] For the DPPH solution, 0.1 mM DPPH was prepared by dissolving it in ethanol, and for the sample solution, 100 μL of DPPH solution was mixed with 100 μL of DPPH solution.

[0139] At this time, after reacting in the dark at room temperature for 30 minutes, the absorbance was measured at 517 nm using a spectrophotometer. Here, the cancellation rate was calculated under the condition [(control group absorbance - sample absorbance) / control group absorbance] × 100.

[0141] 3) Measurement of antioxidant activity at the cellular level

[0142] Human dermal fibroblasts were used as the cell line, and oxidative stress was induced by H2O2 treatment.

[0143] At this time, the amount of ROS generated after TFP pretreatment was measured (using DCFH-DA fluorescent probe), and antioxidant enzyme activity was measured (SOD, CAT, GSH-Px).

[0145] [Table 3]

[0146]

[0148] [Table 4]

[0149]

[0150] As shown in Tables 3 and 4, TFP(500 ㎍ It was confirmed that ( / mL) showed 73% DPPH scavenging activity, exhibiting excellent antioxidant activity close to the scavenging activity (78%) of L-ascorbic acid (vitamin C) at the same concentration.

[0152] 3. Evaluation of the Collagen Synthesis-Promoting Effect of TFP

[0153] Table 5 shows the results of measuring the amount of collagen produced during TFP cell proliferation, and Table 6 shows the results of measuring the amount of TGF-β1 protein expression during TFP cell proliferation.

[0155] 1) Cell culture

[0156] Normal Human Dermal Fibroblast (NHDF) was used as the cell line, and the culture conditions were 37℃, 5% CO₂, DMEM medium + 10% FBS, and the number of passages was 3 to 8.

[0158] 2) TFP Treatment and Cell Proliferation Measurement

[0159] TFP concentrations were set at 10, 50, 100, 200, and 500 μg / mL, and treatment times were 24, 48, and 72 hours. Cell proliferation was measured using an MTT assay, with absorbance measured at 570 nm.

[0161] 3) Measurement of collagen synthesis amount

[0162] TFP (200 ㎍ The culture was incubated for 48 hours after treatment ( / mL). During this time, the collagen concentration in the culture medium was measured using a Type I Collagen ELISA Kit. In addition, the expression levels of COL1A1 and COL1A2 mRNA were measured by RT-PCR.

[0164] 4) Analysis of collagen-related protein expression

[0165] TGF-β1 and Smad2 / 3 protein expression were measured using Western blot. In addition, hyaluronic acid synthase (HAS2) expression levels were measured.

[0167] 5) UVA damage protection effect

[0168] TFP was pretreated prior to UVA irradiation (10 J / cm²). In addition, elastin and hyaluronic acid content were measured.

[0170] [Table 5]

[0171]

[0173] [Table 6]

[0174]

[0175] As shown in Tables 5 and 6, the cell proliferation results of TFP (200 μg / mL, 48 hours treatment) showed a cell proliferation rate of 132.4 ± 4.8% (compared to the control group), with statistical significance measured as p < 0.01.

[0176] In addition, the amount of Type I collagen produced (treated at 200 μg / mL TFP) was measured at 118.7 ± 6.2% (an increase compared to the control group), and it was confirmed that mRNA expression levels increased.

[0177] In addition, TGF-β1 protein expression increased by 127.8 ± 5.6%, Smad2 / 3 phosphorylation increased by 134.2 ± 7.1%, and HAS2 expression increased by 115.9 ± 4.8%.

[0178] In addition, when comparing the UVA + TFP treatment group vs. the UVA alone treatment group, the results showed protection of collagen I content: 124.3 ± 6.8 wt%, elastin content: 119.7 ± 5.2 wt%, and hyaluronic acid content: 121.4 ± 4.9 wt%.

[0179] Based on the experimental results above, the TFP-treated group showed a concentration-dependent increase in collagen synthesis, particularly at 500 μg / mL, where it increased to 145% of the control group. At all concentrations, cell viability was maintained at over 90%, confirming the absence of toxicity.

[0180] Therefore, it was found that TFP significantly promotes collagen production in skin fibroblasts, which can contribute to the enhancement of skin elasticity and anti-aging effects.

[0182] 4. Evaluation of the effects of TFP on improving gut health

[0183] Table 7 shows the results of measuring the anti-inflammatory effect according to the high-dose TFP treatment.

[0185] 1) Animal models

[0186] C57BL / 6 mice (6 to 7 weeks old, female) were used as experimental animals. The groups were composed of a control group, a DSS group, a low-dose TFP group (200 mg / kg), and a high-dose TFP group (300 mg / kg).

[0187] The experiment was conducted for 14 days using an oral administration method.

[0189] 2) Measurement of inflammation markers

[0190] Inflammatory markers were measured in serum and colon tissue using cytokine concentration measurement (ELISA).

[0191] The measurement parameters were IL-6, TNF-α, IL-10, and TGF-β, and MPO activity in colon tissue was measured.

[0193] 3) Gut microbiome analysis

[0194] Intestinal microorganisms were analyzed using 16S rRNA gene sequencing. At this time, the relative abundance of major strains was analyzed.

[0196] [Table 7]

[0197]

[0198] As shown in Table 7, the results of the anti-inflammatory effect evaluation showed that when treated with the high dose TFP group (300 mg / kg), IL-6 decreased by 28.4 ± 3.2% (compared to the DSS control group), TNF-α decreased by 22.7 ± 2.8% (compared to the DSS control group), and IL-10 increased by 143.6 ± 8.9% (compared to the DSS control group).

[0199] In addition, the results of the intestinal microbiome improvement evaluation showed that the high-dose TFP group increased. At this time, Lactobacillus increased by 287.4%, Ruminococcaceae was normal at 156.8%, and the harmful bacterium Helicobacter decreased by 34.2%.

[0200] Consequently, it was confirmed that TFP significantly reduces intestinal inflammatory cytokines and promotes the growth of beneficial bacteria, thereby demonstrating an effect on improving gut health.

[0202] 5. Serum Preparation

[0204] 1) Preparation of white wood ear mushroom polysaccharide (TFP) (Preparation Example 1)

[0205] 1 kg of dried fruiting bodies of *Tremella fuciformis* were ground using a grinder. Next, 15 L of purified water was added to the ground *Tremella fuciformis* powder, and hot water extraction was performed at 90°C for 3 hours while stirring. Next, the hot water extracted *Tremella fuciformis* extract was filtered through a 100 mesh filter to remove residue, and the resulting filtrate was concentrated to 1 / 10 of its initial volume at 60°C using a vacuum concentrator. Next, the obtained concentrate was spray-dried using a spray dryer to obtain 450 g of pale yellow TFP powder (yield 45%).

[0207] 2) Preparation of premium anti-aging serum containing TFP (Example 1)

[0208] An anti-aging serum according to Example 1 was prepared by mixing and stirring the composition and composition ratios listed in Table 8 below.

[0210] [Table 8]

[0211]

[0213] 3) Preparation and Efficacy Evaluation of TFP-Containing Moisturizing Serum (0.1wt% TFP)

[0214] A moisturizing serum according to Example 2 was prepared by mixing 0.1 wt% of TFP from Preparation Example 1, 5.0 wt% of glycerin, 3.0 wt% of 1,3-butylene glycol, 2.0 wt% of niacinamide, other basic cosmetic ingredients, and the remaining amount of purified water.

[0215] Subsequently, to measure moisturizing power (moisture content), the 24-hour moisturizing duration was evaluated, and the moisturizing serum according to Example 2 (0.1 wt% TFP) showed a moisturizing duration that was 1.3 to 1.5 times higher than that of Preparation Example 1. This showed a moisture content of approximately 128 to 130% compared to the control group, hyaluronic acid.

[0216] As a result of evaluating the antioxidant effect (DPPH scavenging ability), Example 2 {0.1 wt% TFP (500 μg / mL)} showed a DPPH scavenging ability of about 60 to 65%, indicating significant antioxidant activity.

[0217] In addition, as a result of evaluating collagen synthesis promotion, it was confirmed that fibroblast proliferation in Example 2 (0.1 wt% TFP) increased by about 15 to 20% compared to Preparation Example 1, contributing to the promotion of collagen synthesis.

[0219] 4) Preparation and Efficacy Evaluation of TFP-Containing Skin Toner (0.01wt% TFP)

[0220] A skin toner according to Example 3 was prepared by mixing 0.01 wt% of TFP from Preparation Example 1, 2.0 wt% of glycerin, 0.01 wt% of preservative, and the remaining amount of purified water.

[0221] Subsequently, as a result of evaluating the 4-hour moisturizing retention rate, the skin toner according to Example 3 (0.01 wt% TFP) showed a slight but statistically significant reduction in moisture loss compared to Example 1.

[0222] As a result of evaluating the antioxidant effect, the DPPH scavenging activity was found to be approximately 45 to 50%.

[0224] 5) Manufacture of TFP-containing health functional food jelly sticks

[0225] A jelly stick according to Preparation Example 2 was prepared with the composition and composition ratio of Table 9 below. At this time, the jelly stick was prepared with 20g per pouch.

[0227] [Table 9]

[0228]

[0230] 6) Preparation and functional evaluation of a capsule composition containing 100 mg TFP per daily intake

[0231] Based on a once-daily intake, the TFP powder and excipients were homogeneously mixed to form 100 mg of TFP of Preparation Example 2, 5 billion CFU of probiotics, 100 mg of inulin, 50 mg of vitamin C, and the remaining amount of excipients, and then the probiotics and other ingredients were sequentially mixed and filled into capsules.

[0232] As a result of the functional evaluation after 4 weeks of intake, beneficial intestinal bacteria (Lactobacillus, Ruminococcaceae, etc.) showed a tendency to increase by about 1.5 times compared to before intake.

[0233] In addition, it was confirmed that intestinal inflammation markers (IL-6, TNF-α) showed a tendency to decrease by about 10–15% each, contributing to the improvement of intestinal health.

[0234] The rate of increase in blood sugar after meals tended to be suppressed by about 5 to 8%.

[0236] Although the embodiments and comparative examples of the present invention have been described in detail as above, the scope of the present invention is not limited thereto, and the scope of the present invention extends to those that are substantially equivalent to the embodiments of the present invention. Explanation of the symbols

[0237] S1: Grinding step S2: Hot water extraction step S3: Filtration and vacuum concentration step S4: Spray drying step S5: Stirring step

Claims

Claim 1 A cosmetic composition for moisturizing the skin containing a polysaccharide derived from Tremella fuciformis as an active ingredient, wherein the polysaccharide derived from Tremella fuciformis (TFP) is prepared by grinding a dried fruiting body of Tremella fuciformis to 20 to 40 mesh, adding 10 to 20 times the weight of the raw material in purified water, performing hot water extraction at 80 to 95°C for 2 to 4 hours, filtering, vacuum concentration, and spray drying, and wherein the polysaccharide derived from Tremella fuciformis is included in an amount of 3 to 7 weight% relative to the total weight of the cosmetic composition. Claim 2 In claim 1, the polysaccharide derived from the white wood ear mushroom is a heteropolysaccharide in which glucuronic acid is bonded to a mannose backbone, and has a weight-average molecular weight of 1.5 × 10⁻⁶ as measured by gel permeation chromatography (GPC). 7 Up to 3.5 × 10 7 A cosmetic composition for moisturizing the skin containing a polysaccharide derived from white wood ear mushroom, characterized by having Da, DPPH antioxidant activity of 70% or more, and a moisture content of 130% or more. Claim 3 delete Claim 4 A cosmetic composition for moisturizing the skin comprising a polysaccharide derived from white wood ear mushroom, characterized in that, in claim 1, the cosmetic composition is at least one of a serum, cream, lotion, ampoule, and mask pack formulation. Claim 5 A health functional food composition for improving intestinal health containing a polysaccharide derived from Tremella fuciformis as an active ingredient, wherein the polysaccharide derived from Tremella fuciformis (TFP) is prepared by grinding dried fruiting bodies of Tremella fuciformis to 20 to 40 mesh, adding 10 to 20 times the weight of the raw material in purified water, performing hot water extraction at 80 to 95°C for 2 to 4 hours, filtering, vacuum concentration, and spray drying, and wherein the polysaccharide derived from Tremella fuciformis is included in an amount of 100 to 2,000 mg per daily intake. Claim 6 In claim 5, the polysaccharide derived from white wood ear mushroom is 1.5 × 10 7 Up to 3.5 × 10 7 A health functional food composition for improving gut health comprising a polysaccharide derived from white wood ear mushroom, characterized by having a molecular weight of Da, DPPH antioxidant activity of 70% or more, and a moisture content of 130% or more. Claim 7 delete Claim 8 A health functional food composition for improving intestinal health comprising a polysaccharide derived from white wood ear mushroom, characterized in that, in claim 5, the health functional food composition comprises at least one of a capsule, granule, powder, pill, liquid, and jelly formulation. Claim 9 (A) a step of grinding dried white wood ear mushrooms to 20 to 40 mesh; (B) a step of hot water extraction of the ground white wood ear mushrooms with 10 to 20 times the amount of purified water at 80 to 95°C for 2 to 4 hours; (C) a step of filtering and vacuum concentrating the hot water extracted extract; (D) a step of powdering the vacuum-concentrated product by spray drying to obtain a polysaccharide derived from white wood ear mushrooms; and (E) a step of adding the polysaccharide derived from white wood ear mushrooms to cosmetic raw materials and distilled water and stirring to form a cosmetic composition; characterized by comprising a method for preparing a cosmetic composition for skin moisturizing containing a polysaccharide derived from white wood ear mushrooms. Claim 10 A method for preparing a skin moisturizing cosmetic composition containing a polysaccharide derived from white wood ear mushroom, characterized in that, in step (D) of claim 9, the spray drying is performed under conditions of 150 to 180°C. Claim 11 A method for preparing a skin moisturizing cosmetic composition containing a polysaccharide derived from white wood ear mushroom, characterized in that, in step (E) above, the polysaccharide derived from white wood ear mushroom is added in an amount of 0.1 to 10 weight% with respect to the total weight of the cosmetic composition.

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